Rroxscaffold_7G00214220

Protein trichome birefringence-like 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003034
Physical Location & Seq
Reverse (-)
64797377 .. 64799944
2568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00214220.1

Sequence Viewer

Length: 453 bp
ATGTACAGAGACACTAGCATTGGGTTTATTGGTGATTCCTTGAACCGGAATATGTTTGTTTCGCTGTTCTGTTCGCTGAAACGCGTGTCGAGTGAAGTAAAGAAGTGGCGGCCGGTTGGTGCTGATCGCGGGTTCACATTTCTTCAGTACAATGTTACGCTTGCGTATCATCGGACAAATCTTTTGGCTCGTTACGGTAGGTGGTCAGCCAGTTCTAATGGTGGCGTGCTGGAATCTCTTGGATATAAAGAGGGTTATAGAGTTGATGTTGATATTCCGGTTGACACATGGGCAGAATCTCTAAGCTTTCATGACATTCTGATATTCAACACAGGGCATTGGTATTGGGCTCCTGCAAAGTTTGACCCTATGAAGTCGCCTCTGCTTTTTTTGAAAATGGTCAGCCTGTGGTTCCTCCAGTACTTCCTGATGTTGGCCTCGATATGGTTATGA

Protein Analysis

150

Amino Acids

17.45

Weight (kDa)

9.27

Isoelectric Point (pI)

26.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 2 - 120 5e-22 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 84, 129
AciI CCGC 2 cut(s) 109, 129
AcoI YGGCCR 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 128
AfaI GTAC 3 cut(s) 5, 149, 422
AfiI CCNNNNNNNGG 3 cut(s) 45, 433, 444
AflIII ACRYGT 1 cut(s) 82
AgsI TTSAA 3 cut(s) 43, 328, 394
AluBI AGCT 1 cut(s) 306
AluI AGCT 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 3
AoxI GGCC 2 cut(s) 110, 435
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
AsuHPI GGTGA 1 cut(s) 44
BanII GRGCYC 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 3
BfaI CTAG 1 cut(s) 15
BisI GCNGC 1 cut(s) 110
BlsI GCNGC 1 cut(s) 111
BmcAI AGTACT 1 cut(s) 422
BmiI GGNNCC 2 cut(s) 351, 413
BpmI CTGGAG 1 cut(s) 401
BsaWI WCCGGW 2 cut(s) 45, 277
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 433, 444
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 2 cut(s) 210, 418
BseLI CCNNNNNNNGG 3 cut(s) 45, 433, 444
BseNI ACTGG 2 cut(s) 210, 418
BseX3I CGGCCG 1 cut(s) 110
Bsh1236I CGCG 2 cut(s) 84, 129
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 2 cut(s) 112, 437
BsiEI CGRYCG 1 cut(s) 113
BsiSI CCGG 3 cut(s) 46, 113, 278
BslI CCNNNNNNNGG 3 cut(s) 45, 433, 444
BsmAI GTCTC 1 cut(s) 3
BsnI GGCC 2 cut(s) 112, 437
Bsp1286I GDGCHC 1 cut(s) 352
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 124
BspACI CCGC 2 cut(s) 109, 129
BspANI GGCC 2 cut(s) 112, 437
BspFNI CGCG 2 cut(s) 84, 129
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 2 cut(s) 351, 413
BsrFI RCCGGY 1 cut(s) 112
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 210, 418
BssAI RCCGGY 1 cut(s) 112
BssMI GATC 1 cut(s) 124
Bst4CI ACNGT 1 cut(s) 197
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 162, 227
BstDEI CTNAG 1 cut(s) 302
BstFNI CGCG 2 cut(s) 84, 129
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 1 cut(s) 3
BstMBI GATC 1 cut(s) 124
BstMCI CGRYCG 1 cut(s) 113
BstUI CGCG 2 cut(s) 84, 129
BstZI CGGCCG 1 cut(s) 110
BsuRI GGCC 2 cut(s) 112, 437
Cac8I GCNNGC 2 cut(s) 162, 227
CciI TCATGA 1 cut(s) 310
Cfr10I RCCGGY 1 cut(s) 112
Csp6I GTAC 3 cut(s) 4, 148, 421
CviAII CATG 2 cut(s) 288, 311
CviJI RGCY 7 cut(s) 112, 188, 209, 306, 350, 405, 437
CviKI_1 RGCY 7 cut(s) 112, 188, 209, 306, 350, 405, 437
CviQI GTAC 3 cut(s) 4, 148, 421
DdeI CTNAG 1 cut(s) 302
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 110
EagI CGGCCG 1 cut(s) 110
EclXI CGGCCG 1 cut(s) 110
Eco24I GRGCYC 1 cut(s) 352
Eco52I CGGCCG 1 cut(s) 110
Eco57I CTGAAG 1 cut(s) 128
EcoT38I GRGCYC 1 cut(s) 352
FaeI CATG 2 cut(s) 291, 314
FaiI YATR 8 cut(s) 53, 246, 258, 289, 312, 371, 445, 451
FatI CATG 2 cut(s) 287, 310
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 1 cut(s) 110
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 1 cut(s) 110
FspBI CTAG 1 cut(s) 15
GluI GCNGC 1 cut(s) 110
GsuI CTGGAG 1 cut(s) 401
HaeIII GGCC 2 cut(s) 112, 437
HapII CCGG 3 cut(s) 46, 113, 278
Hin1II CATG 2 cut(s) 291, 314
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 3 cut(s) 35, 233, 296
HpaII CCGG 3 cut(s) 46, 113, 278
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 2 cut(s) 135, 283
Hpy188I TCNGA 2 cut(s) 174, 321
Hpy188III TCNNGA 2 cut(s) 311, 427
Hpy8I GTNNAC 2 cut(s) 135, 283
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4V TGCA 1 cut(s) 356
HpyF3I CTNAG 1 cut(s) 302
Hsp92II CATG 2 cut(s) 291, 314
Kzo9I GATC 1 cut(s) 124
LmnI GCTCC 1 cut(s) 355
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 2 cut(s) 154, 191
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 1 cut(s) 134
MhlI GDGCHC 1 cut(s) 352
MluI ACGCGT 1 cut(s) 82
MnlI CCTC 4 cut(s) 244, 390, 425, 448
MspI CCGG 3 cut(s) 46, 113, 278
MvnI CGCG 2 cut(s) 84, 129
NdeII GATC 1 cut(s) 124
NlaIII CATG 2 cut(s) 291, 314
NlaIV GGNNCC 2 cut(s) 351, 413
PagI TCATGA 1 cut(s) 310
PfeI GAWTC 3 cut(s) 35, 233, 296
PkrI GCNGC 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 351, 413
RsaI GTAC 3 cut(s) 5, 149, 422
RsaNI GTAC 3 cut(s) 4, 148, 421
SatI GCNGC 1 cut(s) 110
Sau3AI GATC 1 cut(s) 124
ScaI AGTACT 1 cut(s) 422
SduI GDGCHC 1 cut(s) 352
SetI ASST 2 cut(s) 203, 308
SsiI CCGC 2 cut(s) 109, 129
SspMI CTAG 1 cut(s) 15
TaaI ACNGT 1 cut(s) 197
TaqI TCGA 2 cut(s) 89, 440
TatI WGTACW 3 cut(s) 3, 147, 420
TauI GCSGC 1 cut(s) 112
TfiI GAWTC 3 cut(s) 35, 233, 296
TspDTI ATGAA 2 cut(s) 299, 386
XspI CTAG 1 cut(s) 15
ZrmI AGTACT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.