pycom05g17960

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003318
Physical Location & Seq
Forward (+)
21050476 .. 21052855
2380 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1380 bp
ATGGCTTCACGAACACATTTGTTATGGTCATTTGTTGTTGTTTTTGTTGTTTCCTTTAGTACTACTTCATGTTACGGTAGTTTCCAGGAGGTCAACTCACTTCATAGTTACATTGACCATGTTGATAAAGAGTCTGGCTACAATCCTAGGGCTTATCCTTCTTACATGGACACCACTGAAGGTTTCAAGTCCATGGAATTGATCAGACCAAGAACTCAGCTCTTCAGTTCAAGGAAGATCAACAAAATCGCCGGTGGCATAGCGACATCATCAGCTCCGGCCAAAACGATTAGCGTTGACGATTTTGGAGCTAAAGGGAATGGTGCTGATGATGACACACAGGCATTTTTGAAGGCATGGAAGGTAGCTTGTTCTTCCAGTGGAGCAATAGTTCTTGTGGTGCCACAGAAAAGCTATCTTGTTAGGCCAATTGAATTCTCAGGCCCCTGCAAATCTCGACTTACAATGCAGATTTATGGAACCATAGAAGCATCAGAAGACCGATCAATCTACAAAGACCTAGACCGCTGGCTCATGTTTGACAACGTCCAAAACTTACTAGTTGTTGGTCCCGGAACCATCAATGGCAATGGAAACATATGGTGGAAAAACTCATGCAAAAGAAAGCCTCAGCCCCCTTGCGGTAAACAAGCCCCCACGGCTGTGACCTTCAACGGGTGCAATAACTTGGTGGTGAAGAATCTGAAGATCCAAGACGCTCAACAAATGCATGTTATGTTCCAAAACTGCTTCAATGTTCAAGCTTCCCGTCTCACAGTAACTGCACCTGAGGATAGCCCCAATACTGATGGAATTCATGTTGCAAATACCCAAAACATCACTATCTCGAGCTCGGTTATAGGAACAGGTGATGACTGTATTTCTATTGTGAGTGGGTCTCAAAGAGTTCAAGCCAAAAACATAACTTGTGGGCCAGGCCATGGAATCAGTATTGGTAGCTTGGGAAAAGATGGCTCAAAAGACTATGTTTCAGGAGTACTTGTGAATGGAGCTAAGCTTTCAGGAACCACCAATGGACTCAGGATCAAGACATGGCAGGGTGGCTTAGGCAGTGCAACCAACATTGTTTTCCAGAATGTGCAAATGAATAATGTCACCAACCCCATCATGATCGACCAGAACTACTGTGACCACAAAACCAAAGACTGCAATCAACAGAAATCGGCGGTTCAAGTGAAAAATGTGTTGTACCAAAACATCAGAGGAACAAGTGCTTCCGGCGACGCGATAACGGTTAACTGCAGCCAAAGTTTTCCTTGTCAAGGGATTGTGCTGCAAAATATTCAACTGCAGAATGGAAGAGCTGAATGCAACAATGTTCAGCCTGCTTACAAAGGAGTTGTCTCTCCAAGATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

460

Amino Acids

49.74

Weight (kDa)

8.93

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 124 - 448 2.5e-102 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 400
AccB7I CCANNNNNTGG 1 cut(s) 941
AccII CGCG 1 cut(s) 1247
AciI CCGC 3 cut(s) 526, 642, 1187
AclWI GGATC 2 cut(s) 703, 1052
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 2 cut(s) 434, 813
AcuI CTGAAG 3 cut(s) 198, 208, 725
AfaI GTAC 3 cut(s) 61, 999, 1211
AfiI CCNNNNNNNGG 4 cut(s) 641, 675, 941, 1283
AhlI ACTAGT 1 cut(s) 559
AjnI CCWGG 2 cut(s) 84, 934
AjuI GAANNNNNNNTTGG 2 cut(s) 1073, 1105
AleI CACNNNNGTG 1 cut(s) 662
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
Alw21I GWGCWC 1 cut(s) 854
Alw26I GTCTC 3 cut(s) 776, 903, 1369
AlwI GGATC 2 cut(s) 703, 1052
AlwNI CAGNNNCTG 1 cut(s) 782
Ama87I CYCGRG 1 cut(s) 847
AoxI GGCC 5 cut(s) 279, 425, 442, 932, 937
ApeKI GCWGC 2 cut(s) 1263, 1294
ApoI RAATTY 2 cut(s) 434, 813
ArsI GACNNNNNNTTYG 2 cut(s) 1347, 1379
AspA2I CCTAGG 1 cut(s) 146
AspS9I GGNCC 3 cut(s) 443, 569, 932
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 3 cut(s) 706, 881, 1108
AvaI CYCGRG 1 cut(s) 847
AvaII GGWCC 1 cut(s) 569
AvrII CCTAGG 1 cut(s) 146
AxyI CCTNAGG 1 cut(s) 789
BanI GGYRCC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 854
BbsI GAAGAC 1 cut(s) 504
Bbv12I GWGCWC 1 cut(s) 854
BbvCI CCTCAGC 1 cut(s) 630
BbvI GCAGC 2 cut(s) 1275, 1281
BccI CCATC 4 cut(s) 587, 803, 965, 1133
BceAI ACGGC 1 cut(s) 675
BciT130I CCWGG 2 cut(s) 86, 936
BclI TGATCA 1 cut(s) 201
BcnI CCSGG 1 cut(s) 573
BcoDI GTCTC 3 cut(s) 776, 903, 1369
BcuI ACTAGT 1 cut(s) 559
BfaI CTAG 3 cut(s) 147, 521, 560
BfmI CTRYAG 2 cut(s) 1261, 1310
BglI GCCNNNNNGGC 1 cut(s) 659
BisI GCNGC 2 cut(s) 1264, 1295
BlnI CCTAGG 1 cut(s) 146
BlpI GCTNAGC 1 cut(s) 1014
BlsI GCNGC 2 cut(s) 1265, 1296
BmcAI AGTACT 2 cut(s) 61, 999
Bme1390I CCNGG 3 cut(s) 86, 573, 936
Bme18I GGWCC 1 cut(s) 569
BmeT110I CYCGRG 1 cut(s) 847
BmgT120I GGNCC 3 cut(s) 443, 569, 932
BmiI GGNNCC 6 cut(s) 402, 445, 481, 571, 577, 1027
BmrFI CCNGG 3 cut(s) 86, 573, 936
BmsI GCATC 1 cut(s) 500
BpiI GAAGAC 1 cut(s) 504
BplI GAGNNNNNCTC 4 cut(s) 80, 112, 883, 915
Bpu10I CCTNAGC 2 cut(s) 630, 1066
Bpu1102I GCTNAGC 1 cut(s) 1014
BpuMI CCSGG 1 cut(s) 573
BsaI GGTCTC 1 cut(s) 903
BsaJI CCNNGG 4 cut(s) 146, 192, 657, 940
BsaXI ACNNNNNCTCC 4 cut(s) 375, 405, 987, 1017
Bsc4I CCNNNNNNNGG 4 cut(s) 641, 675, 941, 1283
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 1 cut(s) 378
Bse21I CCTNAGG 1 cut(s) 789
Bse3DI GCAATG 1 cut(s) 595
BseBI CCWGG 2 cut(s) 86, 936
BseDI CCNNGG 4 cut(s) 146, 192, 657, 940
BseLI CCNNNNNNNGG 4 cut(s) 641, 675, 941, 1283
BseMI GCAATG 1 cut(s) 595
BseMII CTCAG 5 cut(s) 230, 453, 644, 780, 1054
BseNI ACTGG 1 cut(s) 378
BseXI GCAGC 2 cut(s) 1275, 1281
BsgI GTGCAG 1 cut(s) 768
Bsh1236I CGCG 1 cut(s) 1247
BshFI GGCC 5 cut(s) 281, 427, 444, 934, 939
BshNI GGYRCC 1 cut(s) 400
BsiHKAI GWGCWC 1 cut(s) 854
BsiHKCI CYCGRG 1 cut(s) 847
BsiSI CCGG 4 cut(s) 252, 278, 573, 1239
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 4 cut(s) 641, 675, 941, 1283
BsmAI GTCTC 3 cut(s) 776, 903, 1369
BsmBI CGTCTC 1 cut(s) 776
BsmFI GGGAC 1 cut(s) 555
BsmI GAATGC 1 cut(s) 1334
BsnI GGCC 5 cut(s) 281, 427, 444, 934, 939
Bso31I GGTCTC 1 cut(s) 903
BsoBI CYCGRG 1 cut(s) 847
Bsp1286I GDGCHC 1 cut(s) 854
Bsp143I GATC 6 cut(s) 201, 237, 503, 708, 1044, 1131
Bsp1720I GCTNAGC 1 cut(s) 1014
Bsp19I CCATGG 2 cut(s) 192, 940
BspACI CCGC 3 cut(s) 526, 642, 1187
BspANI GGCC 5 cut(s) 281, 427, 444, 934, 939
BspCNI CTCAG 5 cut(s) 229, 452, 643, 781, 1053
BspFNI CGCG 1 cut(s) 1247
BspHI TCATGA 1 cut(s) 1128
BspLI GGNNCC 6 cut(s) 402, 445, 481, 571, 577, 1027
BspMAI CTGCAG 2 cut(s) 1265, 1314
BspPI GGATC 2 cut(s) 703, 1052
BspQI GCTCTTC 2 cut(s) 227, 1315
BspT107I GGYRCC 1 cut(s) 400
BspTNI GGTCTC 1 cut(s) 903
BsrDI GCAATG 1 cut(s) 595
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 1 cut(s) 378
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 4 cut(s) 146, 192, 657, 940
BssMI GATC 6 cut(s) 201, 237, 503, 708, 1044, 1131
BssT1I CCWWGG 3 cut(s) 146, 192, 940
Bst2UI CCWGG 2 cut(s) 86, 936
Bst4CI ACNGT 5 cut(s) 77, 778, 878, 1148, 1255
Bst6I CTCTTC 2 cut(s) 227, 1315
BstC8I GCNNGC 2 cut(s) 530, 1347
BstDEI CTNAG 7 cut(s) 216, 439, 630, 789, 1014, 1040, 1066
BstDSI CCRYGG 3 cut(s) 192, 657, 940
BstENI CCTNNNNNAGG 1 cut(s) 1281
BstFNI CGCG 1 cut(s) 1247
BstKTI GATC 6 cut(s) 204, 240, 506, 711, 1047, 1134
BstMAI GTCTC 3 cut(s) 776, 903, 1369
BstMBI GATC 6 cut(s) 201, 237, 503, 708, 1044, 1131
BstMWI GCNNNNNNNGC 1 cut(s) 659
BstNI CCWGG 2 cut(s) 86, 936
BstNSI RCATGY 1 cut(s) 734
BstSCI CCNGG 3 cut(s) 84, 571, 934
BstSFI CTRYAG 2 cut(s) 1261, 1310
BstUI CGCG 1 cut(s) 1247
BstV1I GCAGC 2 cut(s) 1275, 1281
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 1 cut(s) 708
BstYI RGATCY 1 cut(s) 708
Bsu36I CCTNAGG 1 cut(s) 789
BsuRI GGCC 5 cut(s) 281, 427, 444, 934, 939
BtgI CCRYGG 3 cut(s) 192, 657, 940
BtsI GCAGTG 1 cut(s) 1078
BtsIMutI CAGTG 3 cut(s) 174, 385, 1078
Cac8I GCNNGC 2 cut(s) 530, 1347
CaiI CAGNNNCTG 1 cut(s) 782
CciI TCATGA 1 cut(s) 1128
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 3 cut(s) 443, 569, 932
CseI GACGC 2 cut(s) 725, 1253
Csp6I GTAC 3 cut(s) 60, 998, 1210
CviQI GTAC 3 cut(s) 60, 998, 1210
DdeI CTNAG 7 cut(s) 216, 439, 630, 789, 1014, 1040, 1066
DpnI GATC 6 cut(s) 203, 239, 505, 710, 1046, 1133
DpnII GATC 6 cut(s) 201, 237, 503, 708, 1044, 1131
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 2 cut(s) 227, 1315
EarI CTCTTC 2 cut(s) 227, 1315
Ecl136II GAGCTC 1 cut(s) 852
Eco130I CCWWGG 3 cut(s) 146, 192, 940
Eco24I GRGCYC 1 cut(s) 854
Eco31I GGTCTC 1 cut(s) 903
Eco47I GGWCC 1 cut(s) 569
Eco53kI GAGCTC 1 cut(s) 852
Eco57I CTGAAG 3 cut(s) 198, 208, 725
Eco81I CCTNAGG 1 cut(s) 789
Eco88I CYCGRG 1 cut(s) 847
EcoICRI GAGCTC 1 cut(s) 852
EcoNI CCTNNNNNAGG 1 cut(s) 1281
EcoO109I RGGNCCY 1 cut(s) 443
EcoRI GAATTC 2 cut(s) 434, 813
EcoRII CCWGG 2 cut(s) 84, 934
EcoT14I CCWWGG 3 cut(s) 146, 192, 940
EcoT22I ATGCAT 1 cut(s) 732
EcoT38I GRGCYC 1 cut(s) 854
ErhI CCWWGG 3 cut(s) 146, 192, 940
Esp3I CGTCTC 1 cut(s) 776
FalI AAGNNNNNCTT 2 cut(s) 1261, 1293
FaqI GGGAC 1 cut(s) 555
FauNDI CATATG 1 cut(s) 599
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 2 cut(s) 1264, 1295
FriOI GRGCYC 1 cut(s) 854
Fsp4HI GCNGC 2 cut(s) 1264, 1295
FspBI CTAG 3 cut(s) 147, 521, 560
GluI GCNGC 2 cut(s) 1264, 1295
HaeIII GGCC 5 cut(s) 281, 427, 444, 934, 939
HapII CCGG 4 cut(s) 252, 278, 573, 1239
HgaI GACGC 2 cut(s) 725, 1253
HincII GTYRAC 3 cut(s) 94, 298, 1258
HindII GTYRAC 3 cut(s) 94, 298, 1258
HindIII AAGCTT 2 cut(s) 762, 1016
HinfI GANTC 4 cut(s) 131, 700, 945, 1038
HpaI GTTAAC 1 cut(s) 1258
HpaII CCGG 4 cut(s) 252, 278, 573, 1239
HphI GGTGA 3 cut(s) 706, 881, 1108
Hpy166II GTNNAC 4 cut(s) 94, 298, 647, 1258
Hpy188I TCNGA 4 cut(s) 206, 496, 705, 1223
Hpy188III TCNNGA 9 cut(s) 9, 456, 847, 993, 1023, 1042, 1048, 1093, 1129
Hpy8I GTNNAC 4 cut(s) 94, 298, 647, 1258
Hpy99I CGWCG 1 cut(s) 1247
HpyAV CCTTC 5 cut(s) 168, 173, 346, 355, 679
HpyCH4III ACNGT 5 cut(s) 77, 778, 878, 1148, 1255
HpyCH4IV ACGT 1 cut(s) 546
HpyF10VI GCNNNNNNNGC 1 cut(s) 659
HpyF3I CTNAG 7 cut(s) 216, 439, 630, 789, 1014, 1040, 1066
HpySE526I ACGT 1 cut(s) 546
Ksp22I TGATCA 1 cut(s) 201
KspAI GTTAAC 1 cut(s) 1258
Kzo9I GATC 6 cut(s) 201, 237, 503, 708, 1044, 1131
LguI GCTCTTC 2 cut(s) 227, 1315
LmnI GCTCC 4 cut(s) 280, 308, 383, 1010
Lsp1109I GCAGC 2 cut(s) 1275, 1281
LweI GCATC 1 cut(s) 500
MaeI CTAG 3 cut(s) 147, 521, 560
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 6 cut(s) 71, 107, 664, 778, 1114, 1148
MalI GATC 6 cut(s) 203, 239, 505, 710, 1046, 1133
MboI GATC 6 cut(s) 201, 237, 503, 708, 1044, 1131
MboII GAAGA 7 cut(s) 214, 247, 366, 509, 709, 718, 1332
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 1 cut(s) 708
MhlI GDGCHC 1 cut(s) 854
MluCI AATT 4 cut(s) 197, 429, 434, 813
MlyI GAGTC 2 cut(s) 140, 1032
MnlI CCTC 4 cut(s) 82, 639, 784, 1217
Mph1103I ATGCAT 1 cut(s) 732
MseI TTAA 1 cut(s) 1257
MslI CAYNNNNRTG 1 cut(s) 662
MspA1I CMGCKG 1 cut(s) 528
MspI CCGG 4 cut(s) 252, 278, 573, 1239
MspR9I CCNGG 3 cut(s) 86, 573, 936
MunI CAATTG 1 cut(s) 429
Mva1269I GAATGC 1 cut(s) 1334
MvaI CCWGG 2 cut(s) 86, 936
MvnI CGCG 1 cut(s) 1247
MwoI GCNNNNNNNGC 1 cut(s) 659
NciI CCSGG 1 cut(s) 573
NcoI CCATGG 2 cut(s) 192, 940
NdeI CATATG 1 cut(s) 599
NdeII GATC 6 cut(s) 201, 237, 503, 708, 1044, 1131
NlaIV GGNNCC 6 cut(s) 402, 445, 481, 571, 577, 1027
NmuCI GTSAC 3 cut(s) 664, 1114, 1148
NsiI ATGCAT 1 cut(s) 732
NspI RCATGY 1 cut(s) 734
OliI CACNNNNGTG 1 cut(s) 662
PaeR7I CTCGAG 1 cut(s) 847
PagI TCATGA 1 cut(s) 1128
PciSI GCTCTTC 2 cut(s) 227, 1315
PctI GAATGC 1 cut(s) 1334
PfeI GAWTC 2 cut(s) 700, 945
PflFI GACNNNGTC 1 cut(s) 545
PflMI CCANNNNNTGG 1 cut(s) 941
PfoI TCCNGGA 2 cut(s) 84, 571
PkrI GCNGC 2 cut(s) 1265, 1296
PleI GAGTC 2 cut(s) 139, 1032
PpsI GAGTC 2 cut(s) 139, 1032
Psp124BI GAGCTC 1 cut(s) 854
Psp6I CCWGG 2 cut(s) 84, 934
PspGI CCWGG 2 cut(s) 84, 934
PspN4I GGNNCC 6 cut(s) 402, 445, 481, 571, 577, 1027
PspPI GGNCC 3 cut(s) 443, 569, 932
PstI CTGCAG 2 cut(s) 1265, 1314
PstNI CAGNNNCTG 1 cut(s) 782
PsuI RGATCY 1 cut(s) 708
PsyI GACNNNGTC 1 cut(s) 545
RsaI GTAC 3 cut(s) 61, 999, 1211
RsaNI GTAC 3 cut(s) 60, 998, 1210
RseI CAYNNNNRTG 1 cut(s) 662
SacI GAGCTC 1 cut(s) 854
SapI GCTCTTC 2 cut(s) 227, 1315
SaqAI TTAA 1 cut(s) 1257
SatI GCNGC 2 cut(s) 1264, 1295
Sau3AI GATC 6 cut(s) 201, 237, 503, 708, 1044, 1131
Sau96I GGNCC 3 cut(s) 443, 569, 932
ScaI AGTACT 2 cut(s) 61, 999
SchI GAGTC 2 cut(s) 140, 1032
ScrFI CCNGG 3 cut(s) 86, 573, 936
SduI GDGCHC 1 cut(s) 854
SfaNI GCATC 1 cut(s) 500
SfcI CTRYAG 2 cut(s) 1261, 1310
Sfr274I CTCGAG 1 cut(s) 847
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 1 cut(s) 569
SlaI CTCGAG 1 cut(s) 847
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 847
SmoI CTYRAG 1 cut(s) 847
SpeI ACTAGT 1 cut(s) 559
Sse9I AATT 4 cut(s) 197, 429, 434, 813
SsiI CCGC 3 cut(s) 526, 642, 1187
SspI AATATT 1 cut(s) 1303
SspMI CTAG 3 cut(s) 147, 521, 560
SstI GAGCTC 1 cut(s) 854
StyD4I CCNGG 3 cut(s) 84, 571, 934
StyI CCWWGG 3 cut(s) 146, 192, 940
TaaI ACNGT 5 cut(s) 77, 778, 878, 1148, 1255
TaiI ACGT 1 cut(s) 549
TaqI TCGA 3 cut(s) 457, 848, 1134
TaqII GACCGA 1 cut(s) 516
TasI AATT 4 cut(s) 197, 429, 434, 813
TatI WGTACW 2 cut(s) 59, 997
TfiI GAWTC 2 cut(s) 700, 945
Tru1I TTAA 1 cut(s) 1257
Tru9I TTAA 1 cut(s) 1257
TscAI CASTG 3 cut(s) 181, 385, 1078
TseFI GTSAC 3 cut(s) 664, 1114, 1148
TseI GCWGC 2 cut(s) 1263, 1294
Tsp45I GTSAC 3 cut(s) 664, 1114, 1148
TspDTI ATGAA 4 cut(s) 57, 92, 806, 1121
TspRI CASTG 3 cut(s) 181, 385, 1078
Tth111I GACNNNGTC 1 cut(s) 545
Van91I CCANNNNNTGG 1 cut(s) 941
VpaK11BI GGWCC 1 cut(s) 569
XagI CCTNNNNNAGG 1 cut(s) 1281
XapI RAATTY 2 cut(s) 434, 813
XceI RCATGY 1 cut(s) 734
XhoI CTCGAG 1 cut(s) 847
XmaJI CCTAGG 1 cut(s) 146
XspI CTAG 3 cut(s) 147, 521, 560
ZrmI AGTACT 2 cut(s) 61, 999
Zsp2I ATGCAT 1 cut(s) 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.