Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0003580

Physical Location & Seq

Genomic Coordinates Reverse (-)

3458050 .. 3460101

Gene Structure 2052 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g02850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2052 bp

ATGACGGACTACCGGATACCGCCGACGATGAATCTATGGACGGACGACAACGCGTCCTTAATGGAGGCGTTTATGAGCTCATCCGATATGGCGTCGTTTTGGGCACCGCCGCCGGCCCAGCCCACACCTCAACCCGCTCCGCTCCAGCCGCAATCCTCGGCGTCCACCTCAGACTACCCCAAGGCCCCTGTCTCCGCCCAATTCCAGCCGTCCGCCACCCCCTTCAACCAGGAGACCCTCATGCAGCGCCTCCAGGCCCTGATCGAAGGCGAGCGCGAGAGCTGGACCTACGCCATCTTCTGGCAGTCTTCCTACGACTACTCCGGCGCCAGCGCCGGCGCCGTTCTTGGATGGGGCGAAGGATTTTATAAAGACGAGCGGGACAAGGTTAAGGCCAAGGCGAAAACGACGACGTCCGCCGCCGAACAAGAGTACCGCAAGAAAGTCCTCCGAGACCTGAATTCCTTGATTTCCGGCGCCGACACGTCAGCAGACGACGCCGTCGTTGATCAGGAAGTGACCGATACTGAGTGGTTCTTTCTGGTGTCCATGACTCAGTCGTTCGTCAACGGCAGCGGGATCCCGGGTCAGGCCTTCTTTCACTCCACCCCGGTCTGGGTTGCTGGACCGGACCGGCTGGCGGCCTCGGCGTGCGAGAGGGCGCGTCAGGGTCAGGTGTTTGGGCTGCAGACTATGGTTTGCGTTCCGACGGCGAATGGGGTTGTGGAGTTGGGCTCGACCGAGCTCATCTACCAGACCTCCGATCTGATGAACAGGGTCAGGTTTTTGTTCAATTTCAACAATTTGGAGGTCGGTTCTTGGCCCATGGCTGCCGCCGCCGATCAGGGCGAGAACGACCCTTCGCTCTGGCTCAACGACCCGTCGACCACCACCGTGGAAGTGAAGGACCCGGTGAATGCTTCGGCACCCACCAGCACCAGCAACCAGCCCATTTCAAAGCCGATTCAGTTCGACAACCACCCGAGCTCCAGTAGCTTATCCGAGAACCCAAGCCCGGTTCAAGTACCACAATTACAGCAGCAAGTACAACAACAGCAGACGCAGAGCTTCTTCCCCAGGGAGCTCAATTTCTCCGACTACAACGGATATGACAGGAGCAGCGTGAAGAATTCGAATTCCAATTCGCATTCGTTGAAGCCCGAATCGGGCGAGATTTTGAATTTCGGGGAGAGCAAGAGGAGCTCTTACAGCGCCAACGGGAAATTGTTCTCGGGGCATTCCCAAATCGCCGCCGCTGAGGATAACAACAGCAAAAAGAAGAGGTCCCCGCCGTCGCTGGGTAGCAACGAGGAGGGGATATTGTCCTTTAGTTCGGGAGTGGTTTTGCCGTCCAGCGGTGTGGCGAAATCCAGCGGCGGTGCCGATTCAGACCATTCGGACCTCGAAGCGTCGGTGGTTCGGGAGGCGGATAGTAGCCGAGTTGTTGACCCCGAAAAACGACCCAGAAAACGGGGACGGAAACCGGCGAATGGGAGAGAAGAGCCACTGAATCACGTGGAAGCAGAGCGGCAAAGAAGAGAGAAGCTTAACCAGAGATTCTACGCGCTCCGAGCAGTAGTCCCAAACGTTTCGAAAATGGACAAAGCGTCGCTGCTCGGAGACGCCATATCCTACATCAACGAGCTCAAGTTGAAGCTGCAGATGGTAGAGACGGATAAAGAGGAGTTGCAGAAGCAATTGGAGTCGATGAACAAGGACTTTCCCAGCAAAGATTCGAGGTCTTCGGGTTCGACCATGTCAGAGCACGAAATGAAAGGGTCTAGCAGTAAATTGCTAGACATGGACATTGACGTGAAGATAATCGGAAGGGACGCCATGATTCGAATTCAGTGTTGCAAGAAGAACCACCCGGCCGCGAGGCTAATGGCGGCTCTCAAGGAGCTCGACTTGGAAGTACACCACGCCAGCGTTTCTGTAGTCAACGATTTGATGATCCAACAAGCCACCGTGAAAGTGGGTAGCCGGATTTACACGCAGGAACAGCTCCGGTTAGCTCTACATTCCAAAGTTGGCGATGCCCGCCAGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006970 GO:0006972 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009651 GO:0009718 GO:0009719 GO:0009725 GO:0009753 GO:0009812 GO:0009813 GO:0009889 GO:0009891 GO:0009893 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042221 GO:0042440 GO:0042538 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043425 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046148 GO:0046283 GO:0046872 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901576 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

HLH bHLH-MYC_N

Protein Analysis

684

Amino Acids

75.11

Weight (kDa)

5.58

Isoelectric Point (pI)

45.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	80 - 264	5.3e-55	bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH	PF00010	504 - 550	4e-12	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	603 - 674	3.5e-09	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003580)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32640
fragaria_vesca	FvH4_1g13600 FvH4_1g13601 FvH4_4g17280 FvH4_5g22100 FvH4_6g24370 FvH4_6g25910
prunus_persica	Prupe.5G035400_v2.0.a1
pyrus_communis	pycom06g02850 pycom16g24430
rosa_chinensis	RchiOBHm_Chr7g0209751
rosa_laevigata	RLG00000003096 RLG00000008856 RLG00000036489 RLG00000036838
rosa_multiflora	Rmu_sc0000161.1_g000024 Rmu_sc0002616.1_g000020
rosa_roxburghii	Rroxscaffold_1G00006980
rosa_rugosa	Rorug05G0432900 Rorug07G0116000
rosa_samantha	Rh4DG124100 Rh5AG516700 Rh5AG518900 Rh5AG519400 Rh5BG540600 Rh5CG563400 Rh5DG551100 Rh5DG551400 Rh5DG551800 Rh7AG249600 Rh7BG243500 Rh7CG265600 Rh7DG255400
rosa_wichuraiana	Rw0G022750 Rw7G021160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	369
AasI	GACNNNNNNGTC	1 cut(s)	500
AatII	GACGTC	1 cut(s)	416
AccB1I	GGYRCC	6 cut(s)	103, 326, 338, 476, 925, 1379
AccB7I	CCANNNNNTGG	1 cut(s)	300
AccBSI	CCGCTC	4 cut(s)	137, 142, 379, 1528
AccI	GTMKAC	1 cut(s)	884
AccII	CGCG	5 cut(s)	53, 276, 664, 1565, 1877
AclI	AACGTT	1 cut(s)	1587
AclWI	GGATC	3 cut(s)	574, 587, 1948
AcoI	YGGCCR	1 cut(s)	1872
AcsI	RAATTY	5 cut(s)	460, 1129, 1135, 1180, 1845
AcvI	CACGTG	1 cut(s)	1516
AcyI	GRCGYC	9 cut(s)	92, 161, 327, 339, 413, 477, 498, 1623, 1833
AfaI	GTAC	4 cut(s)	434, 1026, 1047, 1917
AflIII	ACRYGT	2 cut(s)	51, 483
AgsI	TTSAA	8 cut(s)	226, 793, 799, 957, 1022, 1156, 1180, 1654
AhdI	GACNNNNNGTC	2 cut(s)	52, 1606
AjiI	CACGTC	2 cut(s)	486, 1813
AjnI	CCWGG	3 cut(s)	228, 252, 1076
AleI	CACNNNNGTG	1 cut(s)	893
Alw21I	GWGCWC	8 cut(s)	80, 747, 989, 1086, 1205, 1647, 1767, 1905
Alw26I	GTCTC	5 cut(s)	196, 227, 447, 1614, 1664
AlwI	GGATC	3 cut(s)	574, 587, 1948
AlwNI	CAGNNNCTG	1 cut(s)	259
Ama87I	CYCGRG	3 cut(s)	583, 982, 1231
AoxI	GGCC	8 cut(s)	114, 183, 255, 393, 591, 642, 821, 1872
ApeKI	GCWGC	8 cut(s)	244, 573, 685, 830, 1039, 1119, 1612, 1657
ApoI	RAATTY	5 cut(s)	460, 1129, 1135, 1180, 1845
Asp700I	GAANNNNTTC	1 cut(s)	1226
AspLEI	GCGC	9 cut(s)	249, 276, 329, 335, 341, 479, 664, 1214, 1567
AsuC2I	CCSGG	6 cut(s)	584, 585, 611, 911, 1016, 1871
AsuHPI	GGTGA	1 cut(s)	925
AsuII	TTCGAA	3 cut(s)	1133, 1592, 1843
AvaI	CYCGRG	3 cut(s)	583, 982, 1231
AvaII	GGWCC	6 cut(s)	285, 626, 631, 907, 1284, 1399
BaeGI	GKGCMC	1 cut(s)	106
BaeI	ACNNNNGTAYC	2 cut(s)	416, 449
BamHI	GGATCC	1 cut(s)	579
BanI	GGYRCC	6 cut(s)	103, 326, 338, 476, 925, 1379
BanII	GRGCYC	8 cut(s)	80, 737, 747, 989, 1086, 1205, 1647, 1905
BbrPI	CACGTG	1 cut(s)	1516
BbsI	GAAGAC	2 cut(s)	300, 1734
Bbv12I	GWGCWC	8 cut(s)	80, 747, 989, 1086, 1205, 1647, 1767, 1905
BbvCI	CCTCAGC	1 cut(s)	1257
BbvI	GCAGC	8 cut(s)	256, 585, 672, 817, 1051, 1131, 1599, 1644
BccI	CCATC	3 cut(s)	302, 345, 1657
BceAI	ACGGC	7 cut(s)	193, 326, 485, 586, 726, 1276, 1333
BciT130I	CCWGG	3 cut(s)	230, 254, 1078
BciVI	GTATCC	1 cut(s)	9
BclI	TGATCA	1 cut(s)	508
BcnI	CCSGG	6 cut(s)	584, 585, 611, 911, 1016, 1871
BcoDI	GTCTC	5 cut(s)	196, 227, 447, 1614, 1664
BfaI	CTAG	2 cut(s)	1782, 1796
BfmI	CTRYAG	3 cut(s)	686, 1658, 1935
BfoI	RGCGCY	6 cut(s)	250, 330, 336, 342, 480, 1215
BfuI	GTATCC	1 cut(s)	9
Bme1390I	CCNGG	9 cut(s)	230, 254, 584, 585, 611, 911, 1016, 1078, 1871
Bme18I	GGWCC	6 cut(s)	285, 626, 631, 907, 1284, 1399
BmeRI	GACNNNNNGTC	2 cut(s)	52, 1606
BmeT110I	CYCGRG	3 cut(s)	583, 982, 1231
BmgBI	CACGTC	2 cut(s)	486, 1813
BmrFI	CCNGG	9 cut(s)	230, 254, 584, 585, 611, 911, 1016, 1078, 1871
BmsI	GCATC	1 cut(s)	2026
BpiI	GAAGAC	2 cut(s)	300, 1734
BplI	GAGNNNNNCTC	2 cut(s)	719, 751
BpmI	CTGGAG	3 cut(s)	128, 236, 973
Bpu10I	CCTNAGC	1 cut(s)	1257
Bpu14I	TTCGAA	3 cut(s)	1133, 1592, 1843
BpuEI	CTTGAG	2 cut(s)	1631, 1880
BpuMI	CCSGG	6 cut(s)	584, 585, 611, 911, 1016, 1871
BsaAI	YACGTR	1 cut(s)	1516
BsaHI	GRCGYC	9 cut(s)	92, 161, 327, 339, 413, 477, 498, 1623, 1833
BsaI	GGTCTC	2 cut(s)	227, 447
BsaWI	WCCGGW	3 cut(s)	12, 628, 2007
BsaXI	ACNNNNNCTCC	6 cut(s)	305, 335, 743, 773, 971, 1001
Bse118I	RCCGGY	4 cut(s)	112, 335, 633, 1483
Bse1I	ACTGG	1 cut(s)	990
BseBI	CCWGG	3 cut(s)	230, 254, 1078
BseGI	GGATG	2 cut(s)	80, 356
BseMII	CTCAG	4 cut(s)	183, 519, 569, 1248
BseNI	ACTGG	1 cut(s)	990
BseRI	GAGGAG	3 cut(s)	1213, 1325, 1697
BseSI	GKGCMC	1 cut(s)	106
BseX3I	CGGCCG	1 cut(s)	1872
BseXI	GCAGC	8 cut(s)	256, 585, 672, 817, 1051, 1131, 1599, 1644
BseYI	CCCAGC	3 cut(s)	117, 1297, 1724
Bsh1236I	CGCG	5 cut(s)	53, 276, 664, 1565, 1877
Bsh1285I	CGRYCG	2 cut(s)	741, 1875
BshFI	GGCC	8 cut(s)	116, 185, 257, 395, 593, 644, 823, 1874
BshNI	GGYRCC	6 cut(s)	103, 326, 338, 476, 925, 1379
BsiEI	CGRYCG	2 cut(s)	741, 1875
BsiHKAI	GWGCWC	8 cut(s)	80, 747, 989, 1086, 1205, 1647, 1767, 1905
BsiHKCI	CYCGRG	3 cut(s)	583, 982, 1231
BslFI	GGGAC	5 cut(s)	395, 1270, 1488, 1565, 1844
BsmAI	GTCTC	5 cut(s)	196, 227, 447, 1614, 1664
BsmBI	CGTCTC	2 cut(s)	1614, 1664
BsmFI	GGGAC	5 cut(s)	395, 1270, 1488, 1565, 1844
BsmI	GAATGC	3 cut(s)	922, 1147, 1237
BsnI	GGCC	8 cut(s)	116, 185, 257, 395, 593, 644, 823, 1874
Bso31I	GGTCTC	2 cut(s)	227, 447
BsoBI	CYCGRG	3 cut(s)	583, 982, 1231
Bsp119I	TTCGAA	3 cut(s)	1133, 1592, 1843
Bsp143I	GATC	6 cut(s)	261, 508, 579, 763, 841, 1953
Bsp19I	CCATGG	1 cut(s)	825
BspANI	GGCC	8 cut(s)	116, 185, 257, 395, 593, 644, 823, 1874
BspCNI	CTCAG	4 cut(s)	182, 520, 568, 1249
BspFNI	CGCG	5 cut(s)	53, 276, 664, 1565, 1877
BspMAI	CTGCAG	2 cut(s)	690, 1662
BspPI	GGATC	3 cut(s)	574, 587, 1948
BspQI	GCTCTTC	1 cut(s)	1494
BspT104I	TTCGAA	3 cut(s)	1133, 1592, 1843
BspT107I	GGYRCC	6 cut(s)	103, 326, 338, 476, 925, 1379
BspTNI	GGTCTC	2 cut(s)	227, 447
BsrBI	CCGCTC	4 cut(s)	137, 142, 379, 1528
BsrFI	RCCGGY	4 cut(s)	112, 335, 633, 1483
BsrI	ACTGG	1 cut(s)	990
BssAI	RCCGGY	4 cut(s)	112, 335, 633, 1483
BssMI	GATC	6 cut(s)	261, 508, 579, 763, 841, 1953
BssNI	GRCGYC	9 cut(s)	92, 161, 327, 339, 413, 477, 498, 1623, 1833
BssT1I	CCWWGG	3 cut(s)	180, 396, 825
Bst2UI	CCWGG	3 cut(s)	230, 254, 1078
Bst4CI	ACNGT	2 cut(s)	895, 1969
Bst6I	CTCTTC	3 cut(s)	1274, 1494, 1531
BstACI	GRCGYC	9 cut(s)	92, 161, 327, 339, 413, 477, 498, 1623, 1833
BstBAI	YACGTR	1 cut(s)	1516
BstBI	TTCGAA	3 cut(s)	1133, 1592, 1843
BstC8I	GCNNGC	8 cut(s)	114, 272, 331, 337, 639, 652, 1927, 2041
BstDEI	CTNAG	4 cut(s)	169, 528, 555, 1257
BstDSI	CCRYGG	2 cut(s)	825, 894
BstF5I	GGATG	2 cut(s)	80, 356
BstFNI	CGCG	5 cut(s)	53, 276, 664, 1565, 1877
BstH2I	RGCGCY	6 cut(s)	250, 330, 336, 342, 480, 1215
BstHHI	GCGC	9 cut(s)	249, 276, 329, 335, 341, 479, 664, 1214, 1567
BstKTI	GATC	6 cut(s)	264, 511, 582, 766, 844, 1956
BstMAI	GTCTC	5 cut(s)	196, 227, 447, 1614, 1664
BstMBI	GATC	6 cut(s)	261, 508, 579, 763, 841, 1953
BstMCI	CGRYCG	2 cut(s)	741, 1875
BstNI	CCWGG	3 cut(s)	230, 254, 1078
BstSCI	CCNGG	9 cut(s)	228, 252, 582, 583, 609, 909, 1014, 1076, 1869
BstSFI	CTRYAG	3 cut(s)	686, 1658, 1935
BstSLI	GKGCMC	1 cut(s)	106
BstUI	CGCG	5 cut(s)	53, 276, 664, 1565, 1877
BstV1I	GCAGC	8 cut(s)	256, 585, 672, 817, 1051, 1131, 1599, 1644
BstV2I	GAAGAC	2 cut(s)	300, 1734
BstX2I	RGATCY	1 cut(s)	579
BstXI	CCANNNNNNTGG	2 cut(s)	895, 1360
BstYI	RGATCY	1 cut(s)	579
BstZI	CGGCCG	1 cut(s)	1872
BsuI	GTATCC	1 cut(s)	9
BsuRI	GGCC	8 cut(s)	116, 185, 257, 395, 593, 644, 823, 1874
BtgI	CCRYGG	2 cut(s)	825, 894
BtrI	CACGTC	2 cut(s)	486, 1813
BtsCI	GGATG	2 cut(s)	80, 356
BtsIMutI	CAGTG	2 cut(s)	1505, 1856
Cac8I	GCNNGC	8 cut(s)	114, 272, 331, 337, 639, 652, 1927, 2041
CaiI	CAGNNNCTG	1 cut(s)	259
CfoI	GCGC	9 cut(s)	249, 276, 329, 335, 341, 479, 664, 1214, 1567
Cfr10I	RCCGGY	4 cut(s)	112, 335, 633, 1483
Cfr9I	CCCGGG	1 cut(s)	583
CpoI	CGGWCCG	1 cut(s)	631
Csp6I	GTAC	4 cut(s)	433, 1025, 1046, 1916
CspI	CGGWCCG	1 cut(s)	631
CviAII	CATG	6 cut(s)	241, 550, 826, 1756, 1801, 1837
CviQI	GTAC	4 cut(s)	433, 1025, 1046, 1916
DdeI	CTNAG	4 cut(s)	169, 528, 555, 1257
DinI	GGCGCC	3 cut(s)	328, 340, 478
DpnI	GATC	6 cut(s)	263, 510, 581, 765, 843, 1955
DpnII	GATC	6 cut(s)	261, 508, 579, 763, 841, 1953
DrdI	GACNNNNNNGTC	1 cut(s)	500
DriI	GACNNNNNGTC	2 cut(s)	52, 1606
DseDI	GACNNNNNNGTC	1 cut(s)	500
EaeI	YGGCCR	1 cut(s)	1872
EagI	CGGCCG	1 cut(s)	1872
Eam1104I	CTCTTC	3 cut(s)	1274, 1494, 1531
Eam1105I	GACNNNNNGTC	2 cut(s)	52, 1606
EarI	CTCTTC	3 cut(s)	1274, 1494, 1531
EciI	GGCGGA	4 cut(s)	184, 202, 406, 1442
Ecl136II	GAGCTC	7 cut(s)	78, 745, 987, 1084, 1203, 1645, 1903
EclXI	CGGCCG	1 cut(s)	1872
Eco130I	CCWWGG	3 cut(s)	180, 396, 825
Eco147I	AGGCCT	1 cut(s)	593
Eco24I	GRGCYC	8 cut(s)	80, 737, 747, 989, 1086, 1205, 1647, 1905
Eco31I	GGTCTC	2 cut(s)	227, 447
Eco47I	GGWCC	6 cut(s)	285, 626, 631, 907, 1284, 1399
Eco52I	CGGCCG	1 cut(s)	1872
Eco53kI	GAGCTC	7 cut(s)	78, 745, 987, 1084, 1203, 1645, 1903
Eco72I	CACGTG	1 cut(s)	1516
Eco88I	CYCGRG	3 cut(s)	583, 982, 1231
EcoICRI	GAGCTC	7 cut(s)	78, 745, 987, 1084, 1203, 1645, 1903
EcoO109I	RGGNCCY	4 cut(s)	184, 256, 907, 1284
EcoRI	GAATTC	4 cut(s)	460, 1129, 1135, 1845
EcoRII	CCWGG	3 cut(s)	228, 252, 1076
EcoT14I	CCWWGG	3 cut(s)	180, 396, 825
EcoT38I	GRGCYC	8 cut(s)	80, 737, 747, 989, 1086, 1205, 1647, 1905
EgeI	GGCGCC	3 cut(s)	328, 340, 478
EheI	GGCGCC	3 cut(s)	328, 340, 478
ErhI	CCWWGG	3 cut(s)	180, 396, 825
Esp3I	CGTCTC	2 cut(s)	1614, 1664
FaeI	CATG	6 cut(s)	244, 553, 829, 1759, 1804, 1840
FaqI	GGGAC	5 cut(s)	395, 1270, 1488, 1565, 1844
FatI	CATG	6 cut(s)	240, 549, 825, 1755, 1800, 1836
FauI	CCCGC	5 cut(s)	142, 372, 569, 1296, 2048
FbaI	TGATCA	1 cut(s)	508
FblI	GTMKAC	1 cut(s)	884
FokI	GGATG	2 cut(s)	67, 363
FriOI	GRGCYC	8 cut(s)	80, 737, 747, 989, 1086, 1205, 1647, 1905
FspBI	CTAG	2 cut(s)	1782, 1796
GlaI	GCGC	9 cut(s)	248, 275, 328, 334, 340, 478, 663, 1213, 1566
GsaI	CCCAGC	3 cut(s)	121, 1301, 1728
GsuI	CTGGAG	3 cut(s)	128, 236, 973
HaeII	RGCGCY	6 cut(s)	250, 330, 336, 342, 480, 1215
HaeIII	GGCC	8 cut(s)	116, 185, 257, 395, 593, 644, 823, 1874
HhaI	GCGC	9 cut(s)	249, 276, 329, 335, 341, 479, 664, 1214, 1567
Hin1I	GRCGYC	9 cut(s)	92, 161, 327, 339, 413, 477, 498, 1623, 1833
Hin1II	CATG	6 cut(s)	244, 553, 829, 1759, 1804, 1840
Hin6I	GCGC	9 cut(s)	247, 274, 327, 333, 339, 477, 662, 1212, 1565
HinP1I	GCGC	9 cut(s)	247, 274, 327, 333, 339, 477, 662, 1212, 1565
HincII	GTYRAC	4 cut(s)	568, 885, 1447, 1942
HindII	GTYRAC	4 cut(s)	568, 885, 1447, 1942
HindIII	AAGCTT	1 cut(s)	1544
HphI	GGTGA	1 cut(s)	925
Hpy166II	GTNNAC	6 cut(s)	165, 568, 885, 1447, 1918, 1942
Hpy188III	TCNNGA	3 cut(s)	512, 1335, 1421
Hpy8I	GTNNAC	6 cut(s)	165, 568, 885, 1447, 1918, 1942
HpyAV	CCTTC	7 cut(s)	232, 260, 353, 604, 870, 898, 1821
HpyCH4III	ACNGT	2 cut(s)	895, 1969
HpyCH4IV	ACGT	5 cut(s)	413, 485, 1515, 1587, 1812
HpyCH4V	TGCA	5 cut(s)	244, 688, 1660, 1690, 1857
HpyF3I	CTNAG	4 cut(s)	169, 528, 555, 1257
HpySE526I	ACGT	5 cut(s)	413, 485, 1515, 1587, 1812
Hsp92I	GRCGYC	9 cut(s)	92, 161, 327, 339, 413, 477, 498, 1623, 1833
Hsp92II	CATG	6 cut(s)	244, 553, 829, 1759, 1804, 1840
HspAI	GCGC	9 cut(s)	247, 274, 327, 333, 339, 477, 662, 1212, 1565
KasI	GGCGCC	3 cut(s)	326, 338, 476
KroI	GCCGGC	2 cut(s)	112, 335
KroNI	GCCGGC	2 cut(s)	114, 337
Ksp22I	TGATCA	1 cut(s)	508
Kzo9I	GATC	6 cut(s)	261, 508, 579, 763, 841, 1953
LguI	GCTCTTC	1 cut(s)	1494
LmnI	GCTCC	9 cut(s)	142, 147, 992, 1081, 1116, 1200, 1572, 1900, 2010
Lsp1109I	GCAGC	8 cut(s)	256, 585, 672, 817, 1051, 1131, 1599, 1644
LweI	GCATC	1 cut(s)	2026
MaeI	CTAG	2 cut(s)	1782, 1796
MaeII	ACGT	5 cut(s)	413, 485, 1515, 1587, 1812
MaeIII	GTNAC	1 cut(s)	517
MalI	GATC	6 cut(s)	263, 510, 581, 765, 843, 1955
MbiI	CCGCTC	4 cut(s)	137, 142, 379, 1528
MboI	GATC	6 cut(s)	261, 508, 579, 763, 841, 1953
MfeI	CAATTG	1 cut(s)	1697
MflI	RGATCY	1 cut(s)	579
MluI	ACGCGT	1 cut(s)	51
Mly113I	GGCGCC	3 cut(s)	327, 339, 477
MlyI	GAGTC	2 cut(s)	547, 1712
MmeI	TCCRAC	3 cut(s)	731, 1119, 1981
MreI	CGCCGGCG	1 cut(s)	335
MroNI	GCCGGC	2 cut(s)	112, 335
MroXI	GAANNNNTTC	1 cut(s)	1226
MseI	TTAA	3 cut(s)	59, 390, 1548
MslI	CAYNNNNRTG	2 cut(s)	893, 1811
MspA1I	CMGCKG	4 cut(s)	576, 1256, 1356, 1374
MspR9I	CCNGG	9 cut(s)	230, 254, 584, 585, 611, 911, 1016, 1078, 1871
MunI	CAATTG	1 cut(s)	1697
Mva1269I	GAATGC	3 cut(s)	922, 1147, 1237
MvaI	CCWGG	3 cut(s)	230, 254, 1078
MvnI	CGCG	5 cut(s)	53, 276, 664, 1565, 1877
NaeI	GCCGGC	2 cut(s)	114, 337
NarI	GGCGCC	3 cut(s)	327, 339, 477
NciI	CCSGG	6 cut(s)	584, 585, 611, 911, 1016, 1871
NcoI	CCATGG	1 cut(s)	825
NdeII	GATC	6 cut(s)	261, 508, 579, 763, 841, 1953
NgoMIV	GCCGGC	2 cut(s)	112, 335
NlaIII	CATG	6 cut(s)	244, 553, 829, 1759, 1804, 1840
NmeAIII	GCCGAG	3 cut(s)	137, 626, 1463
NmuCI	GTSAC	1 cut(s)	517
NspV	TTCGAA	3 cut(s)	1133, 1592, 1843
OliI	CACNNNNGTG	1 cut(s)	893
PasI	CCCWGGG	1 cut(s)	1077
PceI	AGGCCT	1 cut(s)	593
PciSI	GCTCTTC	1 cut(s)	1494
PcsI	WCGNNNNNNNCGW	1 cut(s)	501
PctI	GAATGC	3 cut(s)	922, 1147, 1237
PdiI	GCCGGC	2 cut(s)	114, 337
PdmI	GAANNNNTTC	1 cut(s)	1226
PfeI	GAWTC	8 cut(s)	31, 964, 1163, 1385, 1510, 1557, 1733, 1840
PflFI	GACNNNGTC	4 cut(s)	412, 500, 556, 1756
PflMI	CCANNNNNTGG	1 cut(s)	300
PleI	GAGTC	2 cut(s)	547, 1711
PluTI	GGCGCC	3 cut(s)	330, 342, 480
PmaCI	CACGTG	1 cut(s)	1516
PmlI	CACGTG	1 cut(s)	1516
PpsI	GAGTC	2 cut(s)	547, 1711
Ppu21I	YACGTR	1 cut(s)	1516
PpuMI	RGGWCCY	2 cut(s)	907, 1284
PsiI	TTATAA	1 cut(s)	369
Psp124BI	GAGCTC	7 cut(s)	80, 747, 989, 1086, 1205, 1647, 1905
Psp1406I	AACGTT	1 cut(s)	1587
Psp5II	RGGWCCY	2 cut(s)	907, 1284
Psp6I	CCWGG	3 cut(s)	228, 252, 1076
PspCI	CACGTG	1 cut(s)	1516
PspFI	CCCAGC	3 cut(s)	117, 1297, 1724
PspGI	CCWGG	3 cut(s)	228, 252, 1076
PspPPI	RGGWCCY	2 cut(s)	907, 1284
PstI	CTGCAG	2 cut(s)	690, 1662
PstNI	CAGNNNCTG	1 cut(s)	259
PsuI	RGATCY	1 cut(s)	579
PsyI	GACNNNGTC	4 cut(s)	412, 500, 556, 1756
RsaI	GTAC	4 cut(s)	434, 1026, 1047, 1917
RsaNI	GTAC	4 cut(s)	433, 1025, 1046, 1916
RseI	CAYNNNNRTG	2 cut(s)	893, 1811
Rsr2I	CGGWCCG	1 cut(s)	631
RsrII	CGGWCCG	1 cut(s)	631
SacI	GAGCTC	7 cut(s)	80, 747, 989, 1086, 1205, 1647, 1905
SalI	GTCGAC	1 cut(s)	883
SapI	GCTCTTC	1 cut(s)	1494
SaqAI	TTAA	3 cut(s)	59, 390, 1548
Sau3AI	GATC	6 cut(s)	261, 508, 579, 763, 841, 1953
SchI	GAGTC	2 cut(s)	547, 1712
ScrFI	CCNGG	9 cut(s)	230, 254, 584, 585, 611, 911, 1016, 1078, 1871
SfaNI	GCATC	1 cut(s)	2026
SfcI	CTRYAG	3 cut(s)	686, 1658, 1935
SfoI	GGCGCC	3 cut(s)	328, 340, 478
SfuI	TTCGAA	3 cut(s)	1133, 1592, 1843
SgrAI	CRCCGGYG	1 cut(s)	335
SinI	GGWCC	6 cut(s)	285, 626, 631, 907, 1284, 1399
SmaI	CCCGGG	1 cut(s)	585
SmiMI	CAYNNNNRTG	2 cut(s)	893, 1811
SmlI	CTYRAG	2 cut(s)	1646, 1895
SmoI	CTYRAG	2 cut(s)	1646, 1895
SseBI	AGGCCT	1 cut(s)	593
SspDI	GGCGCC	3 cut(s)	326, 338, 476
SspMI	CTAG	2 cut(s)	1782, 1796
SstI	GAGCTC	7 cut(s)	80, 747, 989, 1086, 1205, 1647, 1905
StuI	AGGCCT	1 cut(s)	593
StyD4I	CCNGG	9 cut(s)	228, 252, 582, 583, 609, 909, 1014, 1076, 1869
StyI	CCWWGG	3 cut(s)	180, 396, 825
TaaI	ACNGT	2 cut(s)	895, 1969
TaiI	ACGT	5 cut(s)	416, 488, 1518, 1590, 1815
TaqII	GACCGA	2 cut(s)	536, 755
TatI	WGTACW	2 cut(s)	1045, 1915
TfiI	GAWTC	8 cut(s)	31, 964, 1163, 1385, 1510, 1557, 1733, 1840
Tru1I	TTAA	3 cut(s)	59, 390, 1548
Tru9I	TTAA	3 cut(s)	59, 390, 1548
TscAI	CASTG	2 cut(s)	1512, 1856
TseFI	GTSAC	1 cut(s)	517
TseI	GCWGC	8 cut(s)	244, 573, 685, 830, 1039, 1119, 1612, 1657
Tsp45I	GTSAC	1 cut(s)	517
TspDTI	ATGAA	4 cut(s)	44, 785, 1724, 1787
TspGWI	ACGGA	5 cut(s)	20, 56, 1119, 1492, 1688
TspMI	CCCGGG	1 cut(s)	583
TspRI	CASTG	2 cut(s)	1512, 1856
Tth111I	GACNNNGTC	4 cut(s)	412, 500, 556, 1756
Van91I	CCANNNNNTGG	1 cut(s)	300
VpaK11BI	GGWCC	6 cut(s)	285, 626, 631, 907, 1284, 1399
XapI	RAATTY	5 cut(s)	460, 1129, 1135, 1180, 1845
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1972
XmaI	CCCGGG	1 cut(s)	583
XmiI	GTMKAC	1 cut(s)	884
XmnI	GAANNNNTTC	1 cut(s)	1226
XspI	CTAG	2 cut(s)	1782, 1796
ZraI	GACGTC	1 cut(s)	414

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.