Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0003580

Physical Location & Seq

Genomic Coordinates Reverse (-)

26651396 .. 26653471

Gene Structure 2076 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG255400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2076 bp

ATGACGGACTACCGGATACCTCCCACCATGAATCTCTGGACGGACGACAACGCGTCTCTCATGGAAGCCTTCATGAGCAACTCCGATCTCACCTCCTTCTGGGCCGCCCAGCCGGCCCAGCCCGCAACCCAACCCGCACATCCCCTCCACCAGCCTCAGTCCTCCGCTTCCACCTCCGACTATCCCCGCCCTCCGGCCGCGCAGTCGCAGCCTCCGCCGCCGCCGGCCGCGGCGCCGTTCAACCAGGAGACGCTTATGCAGCGGCTCCAGGCGCTGATCGACGGAGCCAGGGAGAGCTGGACCTACGCCATCTTCTGGCAGTCGTCGTACGAGATGTCCGGAGCTTCCGTGCTCGGATGGGGCGAGGGGTTCTACAAGGACGAGAGGGACAAGGTGAAAGCGAAGCCCAAAACGACGACGTCGTTGGTCGAGCAGGAGTACCGGAAGAAAGTGCTGAGAGACCTCAACTCTTTGATTTCCGGGGCCGAAAACACCGCCGAGGACGACGTCGTGGATCAGGAAGTCACTGATACGGAATGGTTCTTCCTCGTTTCCATGACCCAGAACTTCGTCAACGGCGTCGGGTTGCCGGGTCAGGCTTTTTTTCAGTCCAACCCGGTCTGGGTCGCCGGGCCGGACCGCCTCGCCGACTCGCCGTGCGAGCGGGCCAGACAAGGTCAGGTGTTCGGCCTACAGACTATGGTCTGCGTCCCGACGGCGAATGGGGTCGTCGAGTTGGGCTCGACGGAGCTTATATACCAGAGCGCGGATCTGATGAACAAGGTCCGAGTGTTGTTTGATTTCAATAACCTTGAAGTCGGTTCTTGGCCCATGGGTGGCGCCGCCGACCAGGGAGAGAACGACCCGTCGTCGCTGTGGATCAACGACAACCCGGCCAGCACAATTGAAGTTGTCAAGGACTCGGTGCACATGGGTCCGGCGACTTCAGGACCCAGTACTAGTAACCATCAAATCTCGAAGCCGATTCAGTTCGACAGCCACCCTAGTTCGAGTGGCTTATCGGAGAATCCGAGCGCCGTTCTCCAAGTACAATCTCAGAGCAATCACCACCACCAAGCCCAGCAGCAAGTACAGACGCAGAGCTTCTTCACCAGGGAGCTGAACTTCTCCGATTACAACGGCTACGACGGCAGCAGCGTCAAGAATTCGAATTCGAATTCTCATTCTCATTCCTTGAAGCCGGAATCCGGCGAGATATTGAACTTCGGCGAGAGCAAGAGGACGTCTTACAGTGCCAACAACGGGAAGTTGTTTTCGGCTCAATCCCAAATCGCCGCCGAAGACAACAACAAAAAGAAGAGATCGCCGCCGTCGAGGGGCAGCAACGACGAGGGGATCCTGTCGTTTAGTTCGGGAGTGATTCTACCGTCGAGCAGCGGCGTCGTGAAATCGAGCGCCGGCCCAGTAGATTCCGACCACTCCGACCTCGAAGCCTCCGTGGCTAGGGAGGCCGACAGTAGCAGAGTTGTGGACCCGGAAAAACGACCCCGGAAAAGAGGTCGTAAACCGGCGAACGGCAGAGAGGAGCCGCTGAACCACGTGGAGGCGGAGAGACAGAGAAGAGAGAAGCTCAACCAGAGATTCTATGCTCTCCGAGCTGTCGTCCCAAATGTTTCGAAAATGGACAAGGCATCTCTGCTTGGAGACGCCATCTCTTACATCACCGAGCTCAAGACGAAGCTGCAAACGACGGAGTCCGACAAGGAGGACCTCCACAAGCAAGTGGAGACGTTAAGTAAAGAGCTACAAGAGTCCCGAACGTCTTCGGGTTTGGACCAAGAGCTTAAAGGGTCTAATAAGTTGATCGATTTGGATATTGATGTGAAGATACTCGGATGGGACGCTAGGATTCAAATTCAATGTAGCAAGAAGAACCACCCTGCGGCGAGACTAATGGCGGCGCTGATGGAGCTGGACTTGGATGTACACCATGCCAGCGTCTCGGTAGTCAATGATTTGATGATCCAGCAAGCCACCGTGAGGATGGGCAGCCGGGTTTACACGCAGGAGCAGCTCAGGTTAGCTCTATCAGCCAAAGTGGGGGATGCCAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006970 GO:0006972 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009651 GO:0009718 GO:0009719 GO:0009725 GO:0009753 GO:0009812 GO:0009813 GO:0009889 GO:0009891 GO:0009893 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042221 GO:0042440 GO:0042538 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043425 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046148 GO:0046283 GO:0046872 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901576 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

HLH bHLH-MYC_N

Protein Analysis

691

Amino Acids

75.98

Weight (kDa)

5.5

Isoelectric Point (pI)

46.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	85 - 266	1.4e-54	bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH	PF00010	519 - 565	1e-11	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	613 - 683	3.5e-07	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003580)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32640
fragaria_vesca	FvH4_1g13600 FvH4_1g13601 FvH4_4g17280 FvH4_5g22100 FvH4_6g24370 FvH4_6g25910
prunus_persica	Prupe.5G035400_v2.0.a1
pyrus_communis	pycom06g02850 pycom16g24430
rosa_chinensis	RchiOBHm_Chr7g0209751
rosa_laevigata	RLG00000003096 RLG00000008856 RLG00000036489 RLG00000036838
rosa_multiflora	Rmu_sc0000161.1_g000024 Rmu_sc0002616.1_g000020
rosa_roxburghii	Rroxscaffold_1G00006980
rosa_rugosa	Rorug05G0432900 Rorug07G0116000
rosa_samantha	Rh4DG124100 Rh5AG516700 Rh5AG518900 Rh5AG519400 Rh5BG540600 Rh5CG563400 Rh5DG551100 Rh5DG551400 Rh5DG551800 Rh7AG249600 Rh7BG243500 Rh7CG265600 Rh7DG255400
rosa_wichuraiana	Rw0G022750 Rw7G021160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	3 cut(s)	422, 510, 1247
AccB1I	GGYRCC	2 cut(s)	232, 839
AccB7I	CCANNNNNTGG	1 cut(s)	315
AccBSI	CCGCTC	1 cut(s)	664
AccII	CGCG	4 cut(s)	53, 200, 230, 767
AccIII	TCCGGA	1 cut(s)	338
AclWI	GGATC	6 cut(s)	522, 777, 887, 1351, 1364, 1978
AcoI	YGGCCR	3 cut(s)	195, 225, 894
AcsI	RAATTY	4 cut(s)	1165, 1171, 1177, 1875
AcuI	CTGAAG	1 cut(s)	930
AcvI	CACGTG	1 cut(s)	1561
AcyI	GRCGYC	8 cut(s)	233, 419, 507, 579, 840, 1244, 1401, 1668
AfaI	GTAC	6 cut(s)	329, 440, 958, 1050, 1092, 1947
AflIII	ACRYGT	1 cut(s)	51
AgsI	TTSAA	8 cut(s)	241, 805, 815, 908, 1198, 1222, 1874, 1880
AhdI	GACNNNNNGTC	2 cut(s)	52, 868
AhlI	ACTAGT	1 cut(s)	959
AjnI	CCWGG	5 cut(s)	243, 267, 287, 849, 1112
Alw21I	GWGCWC	3 cut(s)	354, 930, 1692
Alw26I	GTCTC	8 cut(s)	60, 242, 453, 1567, 1659, 1742, 1903, 1966
Alw44I	GTGCAC	1 cut(s)	926
AlwI	GGATC	6 cut(s)	522, 777, 887, 1351, 1364, 1978
AlwNI	CAGNNNCTG	1 cut(s)	274
Aor13HI	TCCGGA	1 cut(s)	338
ApaLI	GTGCAC	1 cut(s)	926
ApoI	RAATTY	4 cut(s)	1165, 1171, 1177, 1875
Asp700I	GAANNNNTTC	1 cut(s)	1783
AspLEI	GCGC	8 cut(s)	202, 235, 274, 767, 842, 1037, 1418, 1924
AsuC2I	CCSGG	8 cut(s)	481, 591, 617, 631, 893, 1496, 1510, 2015
AsuHPI	GGTGA	5 cut(s)	82, 406, 1058, 1102, 1675
AsuII	TTCGAA	3 cut(s)	1169, 1175, 1637
AvaII	GGWCC	8 cut(s)	300, 637, 784, 935, 950, 1492, 1729, 1795
BaeGI	GKGCMC	1 cut(s)	930
BamHI	GGATCC	1 cut(s)	1356
BanI	GGYRCC	2 cut(s)	232, 839
BanII	GRGCYC	2 cut(s)	743, 1692
BbrPI	CACGTG	1 cut(s)	1561
BbsI	GAAGAC	2 cut(s)	1308, 1776
Bbv12I	GWGCWC	3 cut(s)	354, 930, 1692
BccI	CCATC	7 cut(s)	317, 351, 975, 1679, 1851, 1921, 1999
BceAI	ACGGC	9 cut(s)	220, 592, 640, 732, 1022, 1156, 1165, 1315, 1552
BcgI	CGANNNNNNTGC	2 cut(s)	1384, 1418
BciT130I	CCWGG	5 cut(s)	245, 269, 289, 851, 1114
BciVI	GTATCC	1 cut(s)	9
BcnI	CCSGG	8 cut(s)	481, 591, 617, 631, 893, 1496, 1510, 2015
BcoDI	GTCTC	8 cut(s)	60, 242, 453, 1567, 1659, 1742, 1903, 1966
BcuI	ACTAGT	1 cut(s)	959
BfaI	CTAG	4 cut(s)	960, 1005, 1464, 1866
BfmI	CTRYAG	1 cut(s)	692
BfoI	RGCGCY	6 cut(s)	236, 275, 843, 1038, 1419, 1925
BfuI	GTATCC	1 cut(s)	9
BglI	GCCNNNNNGGC	2 cut(s)	113, 1460
BmcAI	AGTACT	1 cut(s)	958
Bme18I	GGWCC	8 cut(s)	300, 637, 784, 935, 950, 1492, 1729, 1795
BmeRI	GACNNNNNGTC	2 cut(s)	52, 868
BmrI	ACTGGG	2 cut(s)	948, 1418
BmsI	GCATC	2 cut(s)	1661, 2056
BmuI	ACTGGG	2 cut(s)	948, 1418
BoxI	GACNNNNGTC	1 cut(s)	701
BpiI	GAAGAC	2 cut(s)	1308, 1776
BplI	GAGNNNNNCTC	4 cut(s)	725, 757, 1575, 1607
BpmI	CTGGAG	1 cut(s)	251
Bpu10I	CCTNAGC	1 cut(s)	2036
Bpu14I	TTCGAA	3 cut(s)	1169, 1175, 1637
BpuEI	CTTGAG	1 cut(s)	1676
BpuMI	CCSGG	8 cut(s)	481, 591, 617, 631, 893, 1496, 1510, 2015
Bsa29I	ATCGAT	1 cut(s)	1827
BsaAI	YACGTR	1 cut(s)	1561
BsaHI	GRCGYC	8 cut(s)	233, 419, 507, 579, 840, 1244, 1401, 1668
BsaI	GGTCTC	1 cut(s)	453
BsaJI	CCNNGG	9 cut(s)	228, 288, 480, 498, 831, 850, 1113, 1458, 1508
BsaWI	WCCGGW	3 cut(s)	12, 338, 441
BsaXI	ACNNNNNCTCC	4 cut(s)	129, 159, 333, 363
Bse118I	RCCGGY	4 cut(s)	112, 223, 1418, 1528
Bse1I	ACTGG	2 cut(s)	954, 1424
BseAI	TCCGGA	1 cut(s)	338
BseBI	CCWGG	5 cut(s)	245, 269, 289, 851, 1114
BseCI	ATCGAT	1 cut(s)	1827
BseDI	CCNNGG	9 cut(s)	228, 288, 480, 498, 831, 850, 1113, 1458, 1508
BseGI	GGATG	6 cut(s)	139, 362, 1862, 1948, 2010, 2071
BseMII	CTCAG	4 cut(s)	170, 446, 1070, 2050
BseNI	ACTGG	2 cut(s)	954, 1424
BseRI	GAGGAG	1 cut(s)	1559
BseSI	GKGCMC	1 cut(s)	930
BseX3I	CGGCCG	2 cut(s)	195, 225
BseYI	CCCAGC	3 cut(s)	108, 117, 1080
Bsh1236I	CGCG	4 cut(s)	53, 200, 230, 767
Bsh1285I	CGRYCG	2 cut(s)	198, 228
BshNI	GGYRCC	2 cut(s)	232, 839
BshVI	ATCGAT	1 cut(s)	1827
BsiEI	CGRYCG	2 cut(s)	198, 228
BsiHKAI	GWGCWC	3 cut(s)	354, 930, 1692
BsiWI	CGTACG	1 cut(s)	327
BslFI	GGGAC	5 cut(s)	401, 695, 1610, 1759, 1874
BsmAI	GTCTC	8 cut(s)	60, 242, 453, 1567, 1659, 1742, 1903, 1966
BsmBI	CGTCTC	5 cut(s)	60, 242, 1659, 1742, 1966
BsmFI	GGGAC	5 cut(s)	401, 695, 1610, 1759, 1874
Bso31I	GGTCTC	1 cut(s)	453
Bsp119I	TTCGAA	3 cut(s)	1169, 1175, 1637
Bsp1286I	GDGCHC	4 cut(s)	354, 743, 930, 1692
Bsp13I	TCCGGA	1 cut(s)	338
Bsp1407I	TGTACA	1 cut(s)	1945
Bsp143I	GATC	9 cut(s)	85, 276, 514, 769, 879, 1322, 1356, 1824, 1983
Bsp19I	CCATGG	1 cut(s)	831
BspCNI	CTCAG	4 cut(s)	169, 447, 1069, 2049
BspDI	ATCGAT	1 cut(s)	1827
BspEI	TCCGGA	1 cut(s)	338
BspFNI	CGCG	4 cut(s)	53, 200, 230, 767
BspHI	TCATGA	1 cut(s)	72
BspPI	GGATC	6 cut(s)	522, 777, 887, 1351, 1364, 1978
BspT104I	TTCGAA	3 cut(s)	1169, 1175, 1637
BspT107I	GGYRCC	2 cut(s)	232, 839
BspTNI	GGTCTC	1 cut(s)	453
BsrBI	CCGCTC	1 cut(s)	664
BsrFI	RCCGGY	4 cut(s)	112, 223, 1418, 1528
BsrGI	TGTACA	1 cut(s)	1945
BsrI	ACTGG	2 cut(s)	954, 1424
BssAI	RCCGGY	4 cut(s)	112, 223, 1418, 1528
BssECI	CCNNGG	9 cut(s)	228, 288, 480, 498, 831, 850, 1113, 1458, 1508
BssMI	GATC	9 cut(s)	85, 276, 514, 769, 879, 1322, 1356, 1824, 1983
BssNI	GRCGYC	8 cut(s)	233, 419, 507, 579, 840, 1244, 1401, 1668
BssT1I	CCWWGG	1 cut(s)	831
Bst2UI	CCWGG	5 cut(s)	245, 269, 289, 851, 1114
Bst4CI	ACNGT	4 cut(s)	1253, 1389, 1478, 1999
Bst6I	CTCTTC	2 cut(s)	1313, 1576
BstACI	GRCGYC	8 cut(s)	233, 419, 507, 579, 840, 1244, 1401, 1668
BstAUI	TGTACA	1 cut(s)	1945
BstBAI	YACGTR	1 cut(s)	1561
BstBI	TTCGAA	3 cut(s)	1169, 1175, 1637
BstC8I	GCNNGC	9 cut(s)	114, 123, 225, 662, 666, 898, 1420, 1957, 1992
BstDEI	CTNAG	4 cut(s)	156, 455, 1056, 2036
BstDSI	CCRYGG	3 cut(s)	228, 831, 1458
BstF5I	GGATG	6 cut(s)	139, 362, 1862, 1948, 2010, 2071
BstFNI	CGCG	4 cut(s)	53, 200, 230, 767
BstH2I	RGCGCY	6 cut(s)	236, 275, 843, 1038, 1419, 1925
BstHHI	GCGC	8 cut(s)	202, 235, 274, 767, 842, 1037, 1418, 1924
BstKTI	GATC	9 cut(s)	88, 279, 517, 772, 882, 1325, 1359, 1827, 1986
BstMAI	GTCTC	8 cut(s)	60, 242, 453, 1567, 1659, 1742, 1903, 1966
BstMBI	GATC	9 cut(s)	85, 276, 514, 769, 879, 1322, 1356, 1824, 1983
BstMCI	CGRYCG	2 cut(s)	198, 228
BstNI	CCWGG	5 cut(s)	245, 269, 289, 851, 1114
BstPAI	GACNNNNGTC	1 cut(s)	701
BstSFI	CTRYAG	1 cut(s)	692
BstSLI	GKGCMC	1 cut(s)	930
BstUI	CGCG	4 cut(s)	53, 200, 230, 767
BstV2I	GAAGAC	2 cut(s)	1308, 1776
BstX2I	RGATCY	2 cut(s)	769, 1356
BstYI	RGATCY	2 cut(s)	769, 1356
BstZI	CGGCCG	2 cut(s)	195, 225
Bsu15I	ATCGAT	1 cut(s)	1827
BsuI	GTATCC	1 cut(s)	9
BsuTUI	ATCGAT	1 cut(s)	1827
BtgI	CCRYGG	3 cut(s)	228, 831, 1458
BtsCI	GGATG	6 cut(s)	139, 362, 1862, 1948, 2010, 2071
BtsIMutI	CAGTG	2 cut(s)	525, 1258
Cac8I	GCNNGC	9 cut(s)	114, 123, 225, 662, 666, 898, 1420, 1957, 1992
CaiI	CAGNNNCTG	1 cut(s)	274
CciI	TCATGA	1 cut(s)	72
CfoI	GCGC	8 cut(s)	202, 235, 274, 767, 842, 1037, 1418, 1924
Cfr10I	RCCGGY	4 cut(s)	112, 223, 1418, 1528
Cfr42I	CCGCGG	1 cut(s)	231
ClaI	ATCGAT	1 cut(s)	1827
CpoI	CGGWCCG	1 cut(s)	637
Csp6I	GTAC	6 cut(s)	328, 439, 957, 1049, 1091, 1946
CspI	CGGWCCG	1 cut(s)	637
CviAII	CATG	7 cut(s)	28, 61, 73, 556, 832, 931, 1952
CviQI	GTAC	6 cut(s)	328, 439, 957, 1049, 1091, 1946
DdeI	CTNAG	4 cut(s)	156, 455, 1056, 2036
DinI	GGCGCC	2 cut(s)	234, 841
DpnI	GATC	9 cut(s)	87, 278, 516, 771, 881, 1324, 1358, 1826, 1985
DpnII	GATC	9 cut(s)	85, 276, 514, 769, 879, 1322, 1356, 1824, 1983
DriI	GACNNNNNGTC	2 cut(s)	52, 868
EaeI	YGGCCR	3 cut(s)	195, 225, 894
EagI	CGGCCG	2 cut(s)	195, 225
Eam1104I	CTCTTC	2 cut(s)	1313, 1576
Eam1105I	GACNNNNNGTC	2 cut(s)	52, 868
EarI	CTCTTC	2 cut(s)	1313, 1576
EciI	GGCGGA	2 cut(s)	204, 1583
Ecl136II	GAGCTC	1 cut(s)	1690
EclXI	CGGCCG	2 cut(s)	195, 225
Eco130I	CCWWGG	1 cut(s)	831
Eco24I	GRGCYC	2 cut(s)	743, 1692
Eco31I	GGTCTC	1 cut(s)	453
Eco47I	GGWCC	8 cut(s)	300, 637, 784, 935, 950, 1492, 1729, 1795
Eco52I	CGGCCG	2 cut(s)	195, 225
Eco53kI	GAGCTC	1 cut(s)	1690
Eco57I	CTGAAG	1 cut(s)	930
Eco72I	CACGTG	1 cut(s)	1561
EcoICRI	GAGCTC	1 cut(s)	1690
EcoO109I	RGGNCCY	2 cut(s)	950, 1729
EcoRI	GAATTC	3 cut(s)	1165, 1171, 1177
EcoRII	CCWGG	5 cut(s)	243, 267, 287, 849, 1112
EcoT14I	CCWWGG	1 cut(s)	831
EcoT38I	GRGCYC	2 cut(s)	743, 1692
EgeI	GGCGCC	2 cut(s)	234, 841
EheI	GGCGCC	2 cut(s)	234, 841
ErhI	CCWWGG	1 cut(s)	831
Esp3I	CGTCTC	5 cut(s)	60, 242, 1659, 1742, 1966
FaeI	CATG	7 cut(s)	31, 64, 76, 559, 835, 934, 1955
FaqI	GGGAC	5 cut(s)	401, 695, 1610, 1759, 1874
FatI	CATG	7 cut(s)	27, 60, 72, 555, 831, 930, 1951
FauI	CCCGC	4 cut(s)	130, 142, 194, 657
FokI	GGATG	5 cut(s)	126, 369, 1869, 1955, 2017
FriOI	GRGCYC	2 cut(s)	743, 1692
FspBI	CTAG	4 cut(s)	960, 1005, 1464, 1866
GlaI	GCGC	8 cut(s)	201, 234, 273, 766, 841, 1036, 1417, 1923
GsaI	CCCAGC	3 cut(s)	112, 121, 1084
GsuI	CTGGAG	1 cut(s)	251
HaeII	RGCGCY	6 cut(s)	236, 275, 843, 1038, 1419, 1925
HhaI	GCGC	8 cut(s)	202, 235, 274, 767, 842, 1037, 1418, 1924
Hin1I	GRCGYC	8 cut(s)	233, 419, 507, 579, 840, 1244, 1401, 1668
Hin1II	CATG	7 cut(s)	31, 64, 76, 559, 835, 934, 1955
Hin6I	GCGC	8 cut(s)	200, 233, 272, 765, 840, 1035, 1416, 1922
HinP1I	GCGC	8 cut(s)	200, 233, 272, 765, 840, 1035, 1416, 1922
HincII	GTYRAC	1 cut(s)	574
HindII	GTYRAC	1 cut(s)	574
HphI	GGTGA	5 cut(s)	82, 406, 1058, 1102, 1675
Hpy166II	GTNNAC	6 cut(s)	574, 928, 1492, 1526, 1948, 2020
Hpy8I	GTNNAC	6 cut(s)	574, 928, 1492, 1526, 1948, 2020
HpyAV	CCTTC	2 cut(s)	79, 106
HpyCH4III	ACNGT	4 cut(s)	1253, 1389, 1478, 1999
HpyCH4IV	ACGT	6 cut(s)	419, 507, 1244, 1560, 1751, 1781
HpyCH4V	TGCA	3 cut(s)	259, 928, 1705
HpyF3I	CTNAG	4 cut(s)	156, 455, 1056, 2036
HpySE526I	ACGT	6 cut(s)	419, 507, 1244, 1560, 1751, 1781
Hsp92I	GRCGYC	8 cut(s)	233, 419, 507, 579, 840, 1244, 1401, 1668
Hsp92II	CATG	7 cut(s)	31, 64, 76, 559, 835, 934, 1955
HspAI	GCGC	8 cut(s)	200, 233, 272, 765, 840, 1035, 1416, 1922
KasI	GGCGCC	2 cut(s)	232, 839
Kpn2I	TCCGGA	1 cut(s)	338
KroI	GCCGGC	3 cut(s)	112, 223, 1418
KroNI	GCCGGC	3 cut(s)	114, 225, 1420
KspI	CCGCGG	1 cut(s)	231
Kzo9I	GATC	9 cut(s)	85, 276, 514, 769, 879, 1322, 1356, 1824, 1983
LmnI	GCTCC	8 cut(s)	270, 284, 341, 748, 1117, 1546, 1930, 2029
LweI	GCATC	2 cut(s)	1661, 2056
MaeI	CTAG	4 cut(s)	960, 1005, 1464, 1866
MaeII	ACGT	6 cut(s)	419, 507, 1244, 1560, 1751, 1781
MaeIII	GTNAC	2 cut(s)	523, 962
MalI	GATC	9 cut(s)	87, 278, 516, 771, 881, 1324, 1358, 1826, 1985
MbiI	CCGCTC	1 cut(s)	664
MboI	GATC	9 cut(s)	85, 276, 514, 769, 879, 1322, 1356, 1824, 1983
MfeI	CAATTG	1 cut(s)	903
MflI	RGATCY	2 cut(s)	769, 1356
MhlI	GDGCHC	4 cut(s)	354, 743, 930, 1692
MluCI	AATT	5 cut(s)	903, 1165, 1171, 1177, 1875
MluI	ACGCGT	1 cut(s)	51
Mly113I	GGCGCC	2 cut(s)	233, 840
MlyI	GAGTC	4 cut(s)	644, 914, 1724, 1781
MmeI	TCCRAC	5 cut(s)	201, 636, 1458, 1467, 1743
MroI	TCCGGA	1 cut(s)	338
MroNI	GCCGGC	3 cut(s)	112, 223, 1418
MroXI	GAANNNNTTC	1 cut(s)	1783
MseI	TTAA	2 cut(s)	1754, 1806
MspA1I	CMGCKG	4 cut(s)	230, 262, 1398, 1552
MunI	CAATTG	1 cut(s)	903
MvaI	CCWGG	5 cut(s)	245, 269, 289, 851, 1114
MvnI	CGCG	4 cut(s)	53, 200, 230, 767
NaeI	GCCGGC	3 cut(s)	114, 225, 1420
NarI	GGCGCC	2 cut(s)	233, 840
NciI	CCSGG	8 cut(s)	481, 591, 617, 631, 893, 1496, 1510, 2015
NcoI	CCATGG	1 cut(s)	831
NdeII	GATC	9 cut(s)	85, 276, 514, 769, 879, 1322, 1356, 1824, 1983
NgoMIV	GCCGGC	3 cut(s)	112, 223, 1418
NlaIII	CATG	7 cut(s)	31, 64, 76, 559, 835, 934, 1955
NmeAIII	GCCGAG	1 cut(s)	523
NmuCI	GTSAC	1 cut(s)	523
NspV	TTCGAA	3 cut(s)	1169, 1175, 1637
PagI	TCATGA	1 cut(s)	72
PcsI	WCGNNNNNNNCGW	7 cut(s)	360, 419, 576, 1028, 1155, 1331, 1455
PdiI	GCCGGC	3 cut(s)	114, 225, 1420
PdmI	GAANNNNTTC	1 cut(s)	1783
PfeI	GAWTC	8 cut(s)	31, 985, 1027, 1205, 1381, 1430, 1602, 1870
Pfl23II	CGTACG	1 cut(s)	327
PflFI	GACNNNGTC	4 cut(s)	418, 506, 675, 1714
PflMI	CCANNNNNTGG	1 cut(s)	315
PleI	GAGTC	4 cut(s)	644, 914, 1723, 1780
PluTI	GGCGCC	2 cut(s)	236, 843
PmaCI	CACGTG	1 cut(s)	1561
PmlI	CACGTG	1 cut(s)	1561
PpsI	GAGTC	4 cut(s)	644, 914, 1723, 1780
Ppu21I	YACGTR	1 cut(s)	1561
PpuMI	RGGWCCY	2 cut(s)	950, 1729
PshAI	GACNNNNGTC	1 cut(s)	701
Psp124BI	GAGCTC	1 cut(s)	1692
Psp5II	RGGWCCY	2 cut(s)	950, 1729
Psp6I	CCWGG	5 cut(s)	243, 267, 287, 849, 1112
PspCI	CACGTG	1 cut(s)	1561
PspFI	CCCAGC	3 cut(s)	108, 117, 1080
PspGI	CCWGG	5 cut(s)	243, 267, 287, 849, 1112
PspLI	CGTACG	1 cut(s)	327
PspPPI	RGGWCCY	2 cut(s)	950, 1729
PstNI	CAGNNNCTG	1 cut(s)	274
PsuI	RGATCY	2 cut(s)	769, 1356
PsyI	GACNNNGTC	4 cut(s)	418, 506, 675, 1714
RsaI	GTAC	6 cut(s)	329, 440, 958, 1050, 1092, 1947
RsaNI	GTAC	6 cut(s)	328, 439, 957, 1049, 1091, 1946
Rsr2I	CGGWCCG	1 cut(s)	637
RsrII	CGGWCCG	1 cut(s)	637
SacI	GAGCTC	1 cut(s)	1692
SacII	CCGCGG	1 cut(s)	231
SaqAI	TTAA	2 cut(s)	1754, 1806
Sau3AI	GATC	9 cut(s)	85, 276, 514, 769, 879, 1322, 1356, 1824, 1983
ScaI	AGTACT	1 cut(s)	958
SchI	GAGTC	4 cut(s)	644, 914, 1724, 1781
SduI	GDGCHC	4 cut(s)	354, 743, 930, 1692
SfaNI	GCATC	2 cut(s)	1661, 2056
SfcI	CTRYAG	1 cut(s)	692
SfoI	GGCGCC	2 cut(s)	234, 841
Sfr303I	CCGCGG	1 cut(s)	231
SfuI	TTCGAA	3 cut(s)	1169, 1175, 1637
SgrBI	CCGCGG	1 cut(s)	231
SinI	GGWCC	8 cut(s)	300, 637, 784, 935, 950, 1492, 1729, 1795
SmlI	CTYRAG	1 cut(s)	1691
SmoI	CTYRAG	1 cut(s)	1691
SpeI	ACTAGT	1 cut(s)	959
Sse9I	AATT	5 cut(s)	903, 1165, 1171, 1177, 1875
SspDI	GGCGCC	2 cut(s)	232, 839
SspMI	CTAG	4 cut(s)	960, 1005, 1464, 1866
SstI	GAGCTC	1 cut(s)	1692
StyI	CCWWGG	1 cut(s)	831
TaaI	ACNGT	4 cut(s)	1253, 1389, 1478, 1999
TaiI	ACGT	6 cut(s)	422, 510, 1247, 1563, 1754, 1784
TasI	AATT	5 cut(s)	903, 1165, 1171, 1177, 1875
TatI	WGTACW	4 cut(s)	956, 1048, 1090, 1945
TfiI	GAWTC	8 cut(s)	31, 985, 1027, 1205, 1381, 1430, 1602, 1870
Tru1I	TTAA	2 cut(s)	1754, 1806
Tru9I	TTAA	2 cut(s)	1754, 1806
TscAI	CASTG	2 cut(s)	532, 1258
TseFI	GTSAC	1 cut(s)	523
Tsp45I	GTSAC	1 cut(s)	523
TspDTI	ATGAA	3 cut(s)	44, 61, 791
TspGWI	ACGGA	8 cut(s)	20, 56, 297, 337, 548, 761, 1447, 1727
TspRI	CASTG	2 cut(s)	532, 1258
Tth111I	GACNNNGTC	4 cut(s)	418, 506, 675, 1714
Van91I	CCANNNNNTGG	1 cut(s)	315
VneI	GTGCAC	1 cut(s)	926
VpaK11BI	GGWCC	8 cut(s)	300, 637, 784, 935, 950, 1492, 1729, 1795
XapI	RAATTY	4 cut(s)	1165, 1171, 1177, 1875
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2002
XmnI	GAANNNNTTC	1 cut(s)	1783
XspI	CTAG	4 cut(s)	960, 1005, 1464, 1866
ZraI	GACGTC	3 cut(s)	420, 508, 1245
ZrmI	AGTACT	1 cut(s)	958

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.