Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0003580

Physical Location & Seq

Genomic Coordinates Reverse (-)

24740087 .. 24746421

Gene Structure 6335 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG249600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2127 bp

ATGAATCTCTGGACGGACGACAACGCGTCTCTCATGGAAGCCTTCATGAGCAACTCCGATCTCACCTCCTTCTGGGCCGCCCAGCCGGCCCAGCCCGCAACCCAACCCGCACATCCCCTCCACCAGCCTCAGTCCTCCGCTTCCACCTCCGACTATCCCCGCCCTCCGGCGGCGCAGTCGCAGTCGCAGTCACAGCCTCCGCCTCCGCCGCCGCCGGCCGCGGCGCCGTTCAACCAGGAGACGCTTATGCAGCGGCTTCAGGCGCTGATCGACGGAGCCAGGGAGAGCTGGACCTACGCCATCTTCTGGCAGTCGTCGTACGAGATGTCCGGAGCTTCCGTGCTCGGATGGGGCGAGGGGTTCTACAAGGACGAGAGGGACAAGGTGAAAGCGAAGCCCAAAACGACGACGTCGTTGGTCGAGCAGGAGTACAGGAAGAAAGTGCTGAGAGACCTCAACTCTTTGATTTCCGGGGCCGACAACACCGCCGAGGACGACGTCGTGGATCAGGAAGTCACTGATACGGAATGGTTCTTCCTCGTTTCCATGACCCAGAACTTCGTCAACGGGGTCGGGTTGCCGGGTCAGGCTTTTTTTCAGTCCAACCCGGTCTGGGTCGCCGGGCCGGACCGCCTCGCCGACTCGCCGTGCGAGCGGGCCAGACAAGGTCAGGTGTTCGGCCTACAGACTATGGTCTGCGTCCCGACGGCGAATGGGGTCGTCGAGTTGGGCTCGACGGAGCTTATATACCAGAGCGCGGATCTGATGAACAAGGTCCGAGTGTTGTTTGATTTCAATAACCTTGAAGTCGGTTCTTGGCCCATGGGTGGCGCCGCCGATCAGGGAGAGAACGACCCGTCGTCGCTGTGGATCAACGACAACCCAGCCAGCACAATTGAAGTTGTCAAGGACTCAGTGCACATGGGTCCGGCGACTTCAGGACCCAGTACTAGTAACCATCAAATCTCGAAGCCGATTCAGTTCGACAGCCACCCTAGTTCGAGTGGCTTATCCGAGAATCCGAGCGCCGTTCTCCAAGTACAATCTCAGAGCAATCACCACCACCAAGCCCAGCAACAAGTACAGACGCAGAGCTTCTTCACCAGGGAGCTGAACTTCTCCGATTACAACGGCTACGACGGCAGCAGCGTCAAGAATTCGAATTCGAATTCTCATTCCTTGAAGCCGGAATCCGGCGAGATATTGAACTTCGGCGAGAGCAAGAGGACATCTTACAGTGCCAACAACGGGAAGTTGTTTTCGGCTCAATCCCAAATCGCCGCCGAAGACAACAACAAAAAGAAGAGATCGCCGCCGTCGAGGGGCAGCAACGACGAGGGGATCCTGTCGTTTAGTTCGGGAGTGATTCTACCGTCGAGCAGCGGCGTCGTGAAATCGAGCGCCGGCCCAGTAGATTCCGACCACTCCGACCTCGAAGCCTCCGTGGCTAGGGAGGCCGACAGTAGCAGAGTTGTGGACCCGGAAAAACGACCCCGGAAAAGAGGTCGTAAACCGGCGAACGGCAGAGAGGAGCCGCTGAACCACGTGGAGGCGGAGAGACAGAGAAGAGAGAAGCTCAACCAGAGATTCTATGCTCTCCGAGCTGTCGTCCCAAATGTTTCGAAAATGGACAAGGCATCTCTGCTTGGAGACGCCATCTCTTACATCACCGAGCTCAAGACGAAGCTGCAAACGACGGAGTCCGACAAGGAGGACCTCCACAAGCAAGTGGAGACGTTAAGTAAAGAGCTACAAGAGTCCCGAACGTCTTCGGGTTTGGACCAAGAGCTTAAAGGGTCTAATAAGTTGATCGATTTGGATATTGATGTGAAGATACTCGGATGGGACGCTAGGATTCAAATTCAATGTAGCAAGAAGAACCACCCTGCCGCGAGACTAATGGCGGCGCTGATGGAGCTGGACTTGGATGTACACCATGCCAGCGTCTCGGTAGTCAATGATTTGATGATCCAGCAAGCCACGGTGAGGATGGGCAGCCGGATTTACACGCAGGAGCAGCTCAGACTCTTAATTGGTGCAAGGCTTTTTCTTGGCCGGCCGGTTAGTTATGCAGAGAAAGATCACGGGATGTTTAAGCTCAAGTGCCTTTGTCAAGGTTGCTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006970 GO:0006972 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009651 GO:0009718 GO:0009719 GO:0009725 GO:0009753 GO:0009812 GO:0009813 GO:0009889 GO:0009891 GO:0009893 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042221 GO:0042440 GO:0042538 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043425 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046148 GO:0046283 GO:0046872 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901576 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

HLH bHLH-MYC_N

Protein Analysis

708

Amino Acids

77.86

Weight (kDa)

5.61

Isoelectric Point (pI)

48.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	82 - 263	1.8e-54	bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH	PF00010	514 - 560	1e-11	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	608 - 666	1.2e-06	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003580)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32640
fragaria_vesca	FvH4_1g13600 FvH4_1g13601 FvH4_4g17280 FvH4_5g22100 FvH4_6g24370 FvH4_6g25910
prunus_persica	Prupe.5G035400_v2.0.a1
pyrus_communis	pycom06g02850 pycom16g24430
rosa_chinensis	RchiOBHm_Chr7g0209751
rosa_laevigata	RLG00000003096 RLG00000008856 RLG00000036489 RLG00000036838
rosa_multiflora	Rmu_sc0000161.1_g000024 Rmu_sc0002616.1_g000020
rosa_roxburghii	Rroxscaffold_1G00006980
rosa_rugosa	Rorug05G0432900 Rorug07G0116000
rosa_samantha	Rh4DG124100 Rh5AG516700 Rh5AG518900 Rh5AG519400 Rh5BG540600 Rh5CG563400 Rh5DG551100 Rh5DG551400 Rh5DG551800 Rh7AG249600 Rh7BG243500 Rh7CG265600 Rh7DG255400
rosa_wichuraiana	Rw0G022750 Rw7G021160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	413, 501
AccB1I	GGYRCC	2 cut(s)	223, 830
AccB7I	CCANNNNNTGG	1 cut(s)	306
AccBSI	CCGCTC	1 cut(s)	655
AccII	CGCG	4 cut(s)	26, 221, 758, 1892
AccIII	TCCGGA	1 cut(s)	329
AclWI	GGATC	6 cut(s)	513, 768, 878, 1336, 1349, 1963
AcoI	YGGCCR	3 cut(s)	216, 2053, 2057
AcsI	RAATTY	4 cut(s)	1156, 1162, 1168, 1860
AcuI	CTGAAG	2 cut(s)	242, 921
AcvI	CACGTG	1 cut(s)	1546
AcyI	GRCGYC	6 cut(s)	224, 410, 498, 831, 1386, 1653
AfaI	GTAC	6 cut(s)	320, 431, 949, 1041, 1083, 1932
AflIII	ACRYGT	1 cut(s)	24
AgsI	TTSAA	8 cut(s)	232, 796, 806, 899, 1183, 1207, 1859, 1865
AhdI	GACNNNNNGTC	2 cut(s)	25, 859
AhlI	ACTAGT	1 cut(s)	950
AjnI	CCWGG	3 cut(s)	234, 278, 1103
Alw21I	GWGCWC	3 cut(s)	345, 921, 1677
Alw26I	GTCTC	8 cut(s)	33, 233, 444, 1552, 1644, 1727, 1888, 1951
Alw44I	GTGCAC	1 cut(s)	917
AlwI	GGATC	6 cut(s)	513, 768, 878, 1336, 1349, 1963
AlwNI	CAGNNNCTG	1 cut(s)	265
Aor13HI	TCCGGA	1 cut(s)	329
ApaLI	GTGCAC	1 cut(s)	917
ApeKI	GCWGC	8 cut(s)	250, 1143, 1146, 1326, 1380, 1687, 1995, 2017
ApoI	RAATTY	4 cut(s)	1156, 1162, 1168, 1860
Asp700I	GAANNNNTTC	1 cut(s)	1768
AspLEI	GCGC	8 cut(s)	175, 226, 265, 758, 833, 1028, 1403, 1909
AsuC2I	CCSGG	6 cut(s)	472, 582, 608, 622, 1481, 1495
AsuHPI	GGTGA	6 cut(s)	55, 397, 1049, 1093, 1660, 1996
AsuII	TTCGAA	3 cut(s)	1160, 1166, 1622
AvaII	GGWCC	8 cut(s)	291, 628, 775, 926, 941, 1477, 1714, 1780
BaeGI	GKGCMC	1 cut(s)	921
BamHI	GGATCC	1 cut(s)	1341
BanI	GGYRCC	2 cut(s)	223, 830
BanII	GRGCYC	2 cut(s)	734, 1677
BbrPI	CACGTG	1 cut(s)	1546
BbsI	GAAGAC	2 cut(s)	1293, 1761
Bbv12I	GWGCWC	3 cut(s)	345, 921, 1677
BbvI	GCAGC	8 cut(s)	262, 1155, 1158, 1338, 1392, 1674, 2007, 2029
BccI	CCATC	7 cut(s)	308, 342, 966, 1664, 1836, 1906, 1984
BceAI	ACGGC	8 cut(s)	211, 631, 723, 1013, 1147, 1156, 1300, 1537
BcgI	CGANNNNNNTGC	2 cut(s)	1369, 1403
BciT130I	CCWGG	3 cut(s)	236, 280, 1105
BcnI	CCSGG	6 cut(s)	472, 582, 608, 622, 1481, 1495
BcoDI	GTCTC	8 cut(s)	33, 233, 444, 1552, 1644, 1727, 1888, 1951
BcuI	ACTAGT	1 cut(s)	950
BfaI	CTAG	4 cut(s)	951, 996, 1449, 1851
BfmI	CTRYAG	1 cut(s)	683
BfoI	RGCGCY	6 cut(s)	227, 266, 834, 1029, 1404, 1910
BglI	GCCNNNNNGGC	2 cut(s)	86, 1445
BmcAI	AGTACT	1 cut(s)	949
Bme1390I	CCNGG	9 cut(s)	236, 280, 472, 582, 608, 622, 1105, 1481, 1495
Bme18I	GGWCC	8 cut(s)	291, 628, 775, 926, 941, 1477, 1714, 1780
BmeRI	GACNNNNNGTC	2 cut(s)	25, 859
BmiI	GGNNCC	9 cut(s)	225, 277, 475, 832, 927, 943, 1343, 1479, 1533
BmrFI	CCNGG	9 cut(s)	236, 280, 472, 582, 608, 622, 1105, 1481, 1495
BmrI	ACTGGG	2 cut(s)	939, 1403
BmsI	GCATC	1 cut(s)	1646
BmuI	ACTGGG	2 cut(s)	939, 1403
BoxI	GACNNNNGTC	1 cut(s)	692
BpiI	GAAGAC	2 cut(s)	1293, 1761
BplI	GAGNNNNNCTC	4 cut(s)	716, 748, 1560, 1592
Bpu14I	TTCGAA	3 cut(s)	1160, 1166, 1622
BpuEI	CTTGAG	2 cut(s)	1661, 2084
BpuMI	CCSGG	6 cut(s)	472, 582, 608, 622, 1481, 1495
Bsa29I	ATCGAT	1 cut(s)	1812
BsaAI	YACGTR	1 cut(s)	1546
BsaHI	GRCGYC	6 cut(s)	224, 410, 498, 831, 1386, 1653
BsaI	GGTCTC	1 cut(s)	444
BsaJI	CCNNGG	9 cut(s)	219, 279, 471, 489, 822, 1104, 1443, 1493, 1980
BsaWI	WCCGGW	1 cut(s)	329
BsaXI	ACNNNNNCTCC	4 cut(s)	102, 132, 324, 354
Bse118I	RCCGGY	6 cut(s)	85, 214, 1403, 1513, 2055, 2059
Bse1I	ACTGG	2 cut(s)	945, 1409
BseAI	TCCGGA	1 cut(s)	329
BseBI	CCWGG	3 cut(s)	236, 280, 1105
BseCI	ATCGAT	1 cut(s)	1812
BseDI	CCNNGG	9 cut(s)	219, 279, 471, 489, 822, 1104, 1443, 1493, 1980
BseGI	GGATG	6 cut(s)	112, 353, 1847, 1933, 1995, 2094
BseMII	CTCAG	5 cut(s)	143, 437, 927, 1061, 2035
BseNI	ACTGG	2 cut(s)	945, 1409
BseRI	GAGGAG	1 cut(s)	1544
BseSI	GKGCMC	1 cut(s)	921
BseX3I	CGGCCG	2 cut(s)	216, 2057
BseXI	GCAGC	8 cut(s)	262, 1155, 1158, 1338, 1392, 1674, 2007, 2029
BseYI	CCCAGC	4 cut(s)	81, 90, 883, 1071
Bsh1236I	CGCG	4 cut(s)	26, 221, 758, 1892
Bsh1285I	CGRYCG	2 cut(s)	219, 2060
BshNI	GGYRCC	2 cut(s)	223, 830
BshVI	ATCGAT	1 cut(s)	1812
BsiEI	CGRYCG	2 cut(s)	219, 2060
BsiHKAI	GWGCWC	3 cut(s)	345, 921, 1677
BsiWI	CGTACG	1 cut(s)	318
BslFI	GGGAC	5 cut(s)	392, 686, 1595, 1744, 1859
BsmAI	GTCTC	8 cut(s)	33, 233, 444, 1552, 1644, 1727, 1888, 1951
BsmBI	CGTCTC	5 cut(s)	33, 233, 1644, 1727, 1951
BsmFI	GGGAC	5 cut(s)	392, 686, 1595, 1744, 1859
Bso31I	GGTCTC	1 cut(s)	444
Bsp119I	TTCGAA	3 cut(s)	1160, 1166, 1622
Bsp1286I	GDGCHC	4 cut(s)	345, 734, 921, 1677
Bsp13I	TCCGGA	1 cut(s)	329
Bsp1407I	TGTACA	1 cut(s)	1930
Bsp19I	CCATGG	1 cut(s)	822
BspCNI	CTCAG	5 cut(s)	142, 438, 926, 1060, 2034
BspDI	ATCGAT	1 cut(s)	1812
BspEI	TCCGGA	1 cut(s)	329
BspFNI	CGCG	4 cut(s)	26, 221, 758, 1892
BspHI	TCATGA	1 cut(s)	45
BspLI	GGNNCC	9 cut(s)	225, 277, 475, 832, 927, 943, 1343, 1479, 1533
BspPI	GGATC	6 cut(s)	513, 768, 878, 1336, 1349, 1963
BspT104I	TTCGAA	3 cut(s)	1160, 1166, 1622
BspT107I	GGYRCC	2 cut(s)	223, 830
BspTNI	GGTCTC	1 cut(s)	444
BsrBI	CCGCTC	1 cut(s)	655
BsrFI	RCCGGY	6 cut(s)	85, 214, 1403, 1513, 2055, 2059
BsrGI	TGTACA	1 cut(s)	1930
BsrI	ACTGG	2 cut(s)	945, 1409
BssAI	RCCGGY	6 cut(s)	85, 214, 1403, 1513, 2055, 2059
BssECI	CCNNGG	9 cut(s)	219, 279, 471, 489, 822, 1104, 1443, 1493, 1980
BssNI	GRCGYC	6 cut(s)	224, 410, 498, 831, 1386, 1653
BssT1I	CCWWGG	1 cut(s)	822
Bst2UI	CCWGG	3 cut(s)	236, 280, 1105
Bst4CI	ACNGT	4 cut(s)	1238, 1374, 1463, 1984
Bst6I	CTCTTC	2 cut(s)	1298, 1561
BstACI	GRCGYC	6 cut(s)	224, 410, 498, 831, 1386, 1653
BstAUI	TGTACA	1 cut(s)	1930
BstBAI	YACGTR	1 cut(s)	1546
BstBI	TTCGAA	3 cut(s)	1160, 1166, 1622
BstDEI	CTNAG	5 cut(s)	129, 446, 913, 1047, 2021
BstDSI	CCRYGG	4 cut(s)	219, 822, 1443, 1980
BstF5I	GGATG	6 cut(s)	112, 353, 1847, 1933, 1995, 2094
BstFNI	CGCG	4 cut(s)	26, 221, 758, 1892
BstH2I	RGCGCY	6 cut(s)	227, 266, 834, 1029, 1404, 1910
BstHHI	GCGC	8 cut(s)	175, 226, 265, 758, 833, 1028, 1403, 1909
BstMAI	GTCTC	8 cut(s)	33, 233, 444, 1552, 1644, 1727, 1888, 1951
BstMCI	CGRYCG	2 cut(s)	219, 2060
BstNI	CCWGG	3 cut(s)	236, 280, 1105
BstPAI	GACNNNNGTC	1 cut(s)	692
BstSCI	CCNGG	9 cut(s)	234, 278, 470, 580, 606, 620, 1103, 1479, 1493
BstSFI	CTRYAG	1 cut(s)	683
BstSLI	GKGCMC	1 cut(s)	921
BstUI	CGCG	4 cut(s)	26, 221, 758, 1892
BstV1I	GCAGC	8 cut(s)	262, 1155, 1158, 1338, 1392, 1674, 2007, 2029
BstV2I	GAAGAC	2 cut(s)	1293, 1761
BstX2I	RGATCY	2 cut(s)	760, 1341
BstYI	RGATCY	2 cut(s)	760, 1341
BstZI	CGGCCG	2 cut(s)	216, 2057
Bsu15I	ATCGAT	1 cut(s)	1812
BsuTUI	ATCGAT	1 cut(s)	1812
BtgI	CCRYGG	4 cut(s)	219, 822, 1443, 1980
BtsCI	GGATG	6 cut(s)	112, 353, 1847, 1933, 1995, 2094
BtsIMutI	CAGTG	3 cut(s)	516, 921, 1243
CaiI	CAGNNNCTG	1 cut(s)	265
CciI	TCATGA	1 cut(s)	45
CfoI	GCGC	8 cut(s)	175, 226, 265, 758, 833, 1028, 1403, 1909
Cfr10I	RCCGGY	6 cut(s)	85, 214, 1403, 1513, 2055, 2059
Cfr42I	CCGCGG	1 cut(s)	222
ClaI	ATCGAT	1 cut(s)	1812
CpoI	CGGWCCG	1 cut(s)	628
CseI	GACGC	9 cut(s)	15, 250, 688, 1096, 1138, 1375, 1661, 1856, 1933
Csp6I	GTAC	6 cut(s)	319, 430, 948, 1040, 1082, 1931
CspI	CGGWCCG	1 cut(s)	628
CviAII	CATG	6 cut(s)	34, 46, 547, 823, 922, 1937
CviQI	GTAC	6 cut(s)	319, 430, 948, 1040, 1082, 1931
DdeI	CTNAG	5 cut(s)	129, 446, 913, 1047, 2021
DinI	GGCGCC	2 cut(s)	225, 832
DriI	GACNNNNNGTC	2 cut(s)	25, 859
EaeI	YGGCCR	3 cut(s)	216, 2053, 2057
EagI	CGGCCG	2 cut(s)	216, 2057
Eam1104I	CTCTTC	2 cut(s)	1298, 1561
Eam1105I	GACNNNNNGTC	2 cut(s)	25, 859
EarI	CTCTTC	2 cut(s)	1298, 1561
EciI	GGCGGA	3 cut(s)	189, 195, 1568
Ecl136II	GAGCTC	1 cut(s)	1675
EclXI	CGGCCG	2 cut(s)	216, 2057
Eco130I	CCWWGG	1 cut(s)	822
Eco24I	GRGCYC	2 cut(s)	734, 1677
Eco31I	GGTCTC	1 cut(s)	444
Eco47I	GGWCC	8 cut(s)	291, 628, 775, 926, 941, 1477, 1714, 1780
Eco52I	CGGCCG	2 cut(s)	216, 2057
Eco53kI	GAGCTC	1 cut(s)	1675
Eco57I	CTGAAG	2 cut(s)	242, 921
Eco72I	CACGTG	1 cut(s)	1546
EcoICRI	GAGCTC	1 cut(s)	1675
EcoO109I	RGGNCCY	2 cut(s)	941, 1714
EcoRI	GAATTC	3 cut(s)	1156, 1162, 1168
EcoRII	CCWGG	3 cut(s)	234, 278, 1103
EcoT14I	CCWWGG	1 cut(s)	822
EcoT38I	GRGCYC	2 cut(s)	734, 1677
EgeI	GGCGCC	2 cut(s)	225, 832
EheI	GGCGCC	2 cut(s)	225, 832
ErhI	CCWWGG	1 cut(s)	822
Esp3I	CGTCTC	5 cut(s)	33, 233, 1644, 1727, 1951
FaeI	CATG	6 cut(s)	37, 49, 550, 826, 925, 1940
FaqI	GGGAC	5 cut(s)	392, 686, 1595, 1744, 1859
FatI	CATG	6 cut(s)	33, 45, 546, 822, 921, 1936
FauI	CCCGC	4 cut(s)	103, 115, 167, 648
FokI	GGATG	6 cut(s)	99, 360, 1854, 1940, 2002, 2101
FriOI	GRGCYC	2 cut(s)	734, 1677
FseI	GGCCGGCC	1 cut(s)	2059
FspBI	CTAG	4 cut(s)	951, 996, 1449, 1851
GlaI	GCGC	8 cut(s)	174, 225, 264, 757, 832, 1027, 1402, 1908
GsaI	CCCAGC	4 cut(s)	85, 94, 887, 1075
HaeII	RGCGCY	6 cut(s)	227, 266, 834, 1029, 1404, 1910
HgaI	GACGC	9 cut(s)	15, 250, 688, 1096, 1138, 1375, 1661, 1856, 1933
HhaI	GCGC	8 cut(s)	175, 226, 265, 758, 833, 1028, 1403, 1909
Hin1I	GRCGYC	6 cut(s)	224, 410, 498, 831, 1386, 1653
Hin1II	CATG	6 cut(s)	37, 49, 550, 826, 925, 1940
Hin6I	GCGC	8 cut(s)	173, 224, 263, 756, 831, 1026, 1401, 1907
HinP1I	GCGC	8 cut(s)	173, 224, 263, 756, 831, 1026, 1401, 1907
HincII	GTYRAC	1 cut(s)	565
HindII	GTYRAC	1 cut(s)	565
HphI	GGTGA	6 cut(s)	55, 397, 1049, 1093, 1660, 1996
Hpy166II	GTNNAC	5 cut(s)	565, 919, 1477, 1511, 1933
Hpy8I	GTNNAC	5 cut(s)	565, 919, 1477, 1511, 1933
HpyAV	CCTTC	2 cut(s)	52, 79
HpyCH4III	ACNGT	4 cut(s)	1238, 1374, 1463, 1984
HpyCH4IV	ACGT	5 cut(s)	410, 498, 1545, 1736, 1766
HpyCH4V	TGCA	5 cut(s)	250, 919, 1690, 2039, 2072
HpyF3I	CTNAG	5 cut(s)	129, 446, 913, 1047, 2021
HpySE526I	ACGT	5 cut(s)	410, 498, 1545, 1736, 1766
Hsp92I	GRCGYC	6 cut(s)	224, 410, 498, 831, 1386, 1653
Hsp92II	CATG	6 cut(s)	37, 49, 550, 826, 925, 1940
HspAI	GCGC	8 cut(s)	173, 224, 263, 756, 831, 1026, 1401, 1907
KasI	GGCGCC	2 cut(s)	223, 830
Kpn2I	TCCGGA	1 cut(s)	329
KroI	GCCGGC	4 cut(s)	85, 214, 1403, 2055
KroNI	GCCGGC	4 cut(s)	87, 216, 1405, 2057
KspI	CCGCGG	1 cut(s)	222
LmnI	GCTCC	8 cut(s)	275, 332, 739, 1108, 1531, 1915, 2014, 2126
Lsp1109I	GCAGC	8 cut(s)	262, 1155, 1158, 1338, 1392, 1674, 2007, 2029
LweI	GCATC	1 cut(s)	1646
MaeI	CTAG	4 cut(s)	951, 996, 1449, 1851
MaeII	ACGT	5 cut(s)	410, 498, 1545, 1736, 1766
MaeIII	GTNAC	3 cut(s)	189, 514, 953
MbiI	CCGCTC	1 cut(s)	655
MfeI	CAATTG	1 cut(s)	894
MflI	RGATCY	2 cut(s)	760, 1341
MhlI	GDGCHC	4 cut(s)	345, 734, 921, 1677
MluCI	AATT	6 cut(s)	894, 1156, 1162, 1168, 1860, 2031
MluI	ACGCGT	1 cut(s)	24
Mly113I	GGCGCC	2 cut(s)	224, 831
MlyI	GAGTC	5 cut(s)	635, 905, 1709, 1766, 2019
MmeI	TCCRAC	5 cut(s)	174, 627, 1443, 1452, 1728
MroI	TCCGGA	1 cut(s)	329
MroNI	GCCGGC	4 cut(s)	85, 214, 1403, 2055
MroXI	GAANNNNTTC	1 cut(s)	1768
MseI	TTAA	4 cut(s)	1739, 1791, 2030, 2094
MspA1I	CMGCKG	4 cut(s)	221, 253, 1383, 1537
MspR9I	CCNGG	9 cut(s)	236, 280, 472, 582, 608, 622, 1105, 1481, 1495
MunI	CAATTG	1 cut(s)	894
MvaI	CCWGG	3 cut(s)	236, 280, 1105
MvnI	CGCG	4 cut(s)	26, 221, 758, 1892
NaeI	GCCGGC	4 cut(s)	87, 216, 1405, 2057
NarI	GGCGCC	2 cut(s)	224, 831
NciI	CCSGG	6 cut(s)	472, 582, 608, 622, 1481, 1495
NcoI	CCATGG	1 cut(s)	822
NgoMIV	GCCGGC	4 cut(s)	85, 214, 1403, 2055
NlaIII	CATG	6 cut(s)	37, 49, 550, 826, 925, 1940
NlaIV	GGNNCC	9 cut(s)	225, 277, 475, 832, 927, 943, 1343, 1479, 1533
NmeAIII	GCCGAG	1 cut(s)	514
NmuCI	GTSAC	2 cut(s)	189, 514
NspV	TTCGAA	3 cut(s)	1160, 1166, 1622
PagI	TCATGA	1 cut(s)	45
PcsI	WCGNNNNNNNCGW	5 cut(s)	351, 410, 1146, 1316, 1440
PdiI	GCCGGC	4 cut(s)	87, 216, 1405, 2057
PdmI	GAANNNNTTC	1 cut(s)	1768
PfeI	GAWTC	8 cut(s)	4, 976, 1018, 1190, 1366, 1415, 1587, 1855
Pfl23II	CGTACG	1 cut(s)	318
PflFI	GACNNNGTC	4 cut(s)	409, 497, 666, 1699
PflMI	CCANNNNNTGG	1 cut(s)	306
PleI	GAGTC	5 cut(s)	635, 905, 1708, 1765, 2019
PluTI	GGCGCC	2 cut(s)	227, 834
PmaCI	CACGTG	1 cut(s)	1546
PmlI	CACGTG	1 cut(s)	1546
PpsI	GAGTC	5 cut(s)	635, 905, 1708, 1765, 2019
Ppu21I	YACGTR	1 cut(s)	1546
PpuMI	RGGWCCY	2 cut(s)	941, 1714
PshAI	GACNNNNGTC	1 cut(s)	692
Psp124BI	GAGCTC	1 cut(s)	1677
Psp5II	RGGWCCY	2 cut(s)	941, 1714
Psp6I	CCWGG	3 cut(s)	234, 278, 1103
PspCI	CACGTG	1 cut(s)	1546
PspFI	CCCAGC	4 cut(s)	81, 90, 883, 1071
PspGI	CCWGG	3 cut(s)	234, 278, 1103
PspLI	CGTACG	1 cut(s)	318
PspN4I	GGNNCC	9 cut(s)	225, 277, 475, 832, 927, 943, 1343, 1479, 1533
PspPPI	RGGWCCY	2 cut(s)	941, 1714
PstNI	CAGNNNCTG	1 cut(s)	265
PsuI	RGATCY	2 cut(s)	760, 1341
PsyI	GACNNNGTC	4 cut(s)	409, 497, 666, 1699
RigI	GGCCGGCC	1 cut(s)	2059
RsaI	GTAC	6 cut(s)	320, 431, 949, 1041, 1083, 1932
RsaNI	GTAC	6 cut(s)	319, 430, 948, 1040, 1082, 1931
Rsr2I	CGGWCCG	1 cut(s)	628
RsrII	CGGWCCG	1 cut(s)	628
SacI	GAGCTC	1 cut(s)	1677
SacII	CCGCGG	1 cut(s)	222
SaqAI	TTAA	4 cut(s)	1739, 1791, 2030, 2094
ScaI	AGTACT	1 cut(s)	949
SchI	GAGTC	5 cut(s)	635, 905, 1709, 1766, 2019
ScrFI	CCNGG	9 cut(s)	236, 280, 472, 582, 608, 622, 1105, 1481, 1495
SduI	GDGCHC	4 cut(s)	345, 734, 921, 1677
SfaNI	GCATC	1 cut(s)	1646
SfcI	CTRYAG	1 cut(s)	683
SfoI	GGCGCC	2 cut(s)	225, 832
Sfr303I	CCGCGG	1 cut(s)	222
SfuI	TTCGAA	3 cut(s)	1160, 1166, 1622
SgrBI	CCGCGG	1 cut(s)	222
SinI	GGWCC	8 cut(s)	291, 628, 775, 926, 941, 1477, 1714, 1780
SmlI	CTYRAG	2 cut(s)	1676, 2099
SmoI	CTYRAG	2 cut(s)	1676, 2099
SpeI	ACTAGT	1 cut(s)	950
Sse9I	AATT	6 cut(s)	894, 1156, 1162, 1168, 1860, 2031
SspDI	GGCGCC	2 cut(s)	223, 830
SspMI	CTAG	4 cut(s)	951, 996, 1449, 1851
SstI	GAGCTC	1 cut(s)	1677
StyD4I	CCNGG	9 cut(s)	234, 278, 470, 580, 606, 620, 1103, 1479, 1493
StyI	CCWWGG	1 cut(s)	822
TaaI	ACNGT	4 cut(s)	1238, 1374, 1463, 1984
TaiI	ACGT	5 cut(s)	413, 501, 1548, 1739, 1769
TasI	AATT	6 cut(s)	894, 1156, 1162, 1168, 1860, 2031
TatI	WGTACW	5 cut(s)	429, 947, 1039, 1081, 1930
TfiI	GAWTC	8 cut(s)	4, 976, 1018, 1190, 1366, 1415, 1587, 1855
Tru1I	TTAA	4 cut(s)	1739, 1791, 2030, 2094
Tru9I	TTAA	4 cut(s)	1739, 1791, 2030, 2094
TscAI	CASTG	3 cut(s)	523, 921, 1243
TseFI	GTSAC	2 cut(s)	189, 514
TseI	GCWGC	8 cut(s)	250, 1143, 1146, 1326, 1380, 1687, 1995, 2017
Tsp45I	GTSAC	2 cut(s)	189, 514
TspDTI	ATGAA	3 cut(s)	17, 34, 782
TspGWI	ACGGA	7 cut(s)	29, 288, 328, 539, 752, 1432, 1712
TspRI	CASTG	3 cut(s)	523, 921, 1243
Tth111I	GACNNNGTC	4 cut(s)	409, 497, 666, 1699
Van91I	CCANNNNNTGG	1 cut(s)	306
VneI	GTGCAC	1 cut(s)	917
VpaK11BI	GGWCC	8 cut(s)	291, 628, 775, 926, 941, 1477, 1714, 1780
XapI	RAATTY	4 cut(s)	1156, 1162, 1168, 1860
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1987
XmnI	GAANNNNTTC	1 cut(s)	1768
XspI	CTAG	4 cut(s)	951, 996, 1449, 1851
ZraI	GACGTC	2 cut(s)	411, 499
ZrmI	AGTACT	1 cut(s)	949

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.