Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0003580

Physical Location & Seq

Genomic Coordinates Forward (+)

25895745 .. 25898207

Gene Structure 2463 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g24430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2298 bp

ATGTTGAGTTCCATGGCATATTTTCTAATAATATCATCACATTCTGTTCCACGCATTGCTGCTCGTGCGCTAAATCTTCAGTTCGTATGCAGTCCGCATAAAATCCCCTTACACAATTCCATACCCTCCCCAACCCAAAATTCTCTCCCCCGCCGCCCCCCCCCCTTCTCTCTCTCTACTTTCTCTCTCTACACACCCAACCAATCCCATCCCCAAAACGCTGCGTATCACATGACGGACTACCGGATACCGCCGACGACGAATCTGTGGACGGACGACAACGCGTCCTTGATGGAGGCGTTTATGAGCTCATCGGATCTGACGTCGTTTTGGGCGGCTCCGCCGGCCCAGCCCACGCCTCAACCTGCTCACGCCCCGGTTCAGCCGCAATCCTCGGCCTCCACTTCTGACTACCCCAAGCCCCCTGTCGCCGCCCAATTCCAGCCCTCCGTCACGCCCTTCAACCAGGAGATGCATGACCAGGAGACGCTCATGCAGCGCCTCCAGGCCCTGATCGAAGGCGCGCGCGAGAGCTGGACCTACGCCATCTTCTGGCAGTCCTCCTACGACAACTCCGGCTCCGCCGTTCTCGGATGGGGGGAAGGATTTTACAAAGACGAGAGGGACAAGGTTAAGGCCAAGGCCAAAACGACGACATCCGCCGCCGAACAAGAGTACCGCAAGAAAGTCCTCCGAGACCTGAACTCCTTGATTTCCGGCGCCGACACGTCAGCAGACGACGCCGTCGTGGATCAAGAAGTGACTGATACTGAGTGGTTCTTTCTCGTTTCCATGACTCAGTCGTTCGTCAACGGCGGCGGGCTTCCGGGTCAGGCCTTTTTTCACTCCACCCCTGTCTGGGTTGCTGGACCGGACCGGCTGGCGGTTTCTCCGTGCGAGCGGGCGCGACAGGGCCAGGTGTTCGGGCTGCAGACTATGGTTTGCGTTCCGACGGCGAATGGGGTTGTGGAGTTGGGATCGACCGAGCTCATCTACCAGAGCTACGATCTGATGAATAAGGTCAGGGTTCTGTTTGATTTCAACAATTTGGAGGTCGGTTCTTGCCCTATGAGCGGCGGCGCCACCGCCGATCAGGGCGAGAACGACCCTTCGTCGCTCTGGCTCAACGATCCGTCGACCACCACCATGGAAGTGAAGGAACCGGTGAATACTTCGGCAACCACCAACACCAGCAACCAGCTAATTTCAAAGCCGGTGCAGTTCGACAACCACCCGAGCTCCAGTAGCTTATCCGAGAACCCGAGCCCAATTCAAGTACCACAATTGCAGCAGCAAGTACAACAACAGCAGCAGACGCAGAGCTTCTTCACCATGGAGCTCAATTTCTCCGATTACGACGGGAGCAGCGTGAAGAATTCCAATTCCAATACGCATTCGTTGAAGCCCGAATCAGGCGAGATTTTGAATTTCGGGGAGAGCAAGAGGAGCTCTTACAGCGCCAATGGGAAATTGTTCTCGGGGCATTCCCAAATTACCGCAGCCGAGGATAACAACAGCAAGAAGAAGAGTTCTCCGCCATCGCGGGGTAGCAACGACGAGGGGATATTGTCCTTTAGTTCGGGAGTGATTTTGCCGTCCAGCTGTGTGGTCAAATCGAGCGGCGGTGCCGATTCAGATCACTCGGACCTCGAAGCGTCGGTGGTTCGGGAGGCGGATAGTAGCCGAGTTGTTGACCCGGAAAAACGACCTAGAAAACGGGGTCGGAAACCGGCGAATGGAAGAGAAGAGCCGCTGAATCACGTGGAAGCAGAGCGGCAAAGAAGAGAGAAGCTTAACCAGAGATTCTACGCCCTCCGAGCAGTAGTCCCAAACGTTTCGAAAATGGACAAAGCGTCGCTGCTCGGAGATGCCATATCTTACATCAACGAGCTCAAGTTGAAGCTGCAGACGGTGGAGACGGATAAGGAGGAGTTACAGAAGCAATTGGAGTCGATGAACAATCACTTGCCCTGCAAAGATTCGAGGTCTTCGGGTTCAATTATGTCAGAGGAGGAACTGAAAGGGTGTAGTAGTAAATTGTTAGACATGGACATTGATGTGAAGATAATCGGGCGGGACGCCATGATTCGAATTCAGTGTTGTAAGAAGAACCACCCGGCCGCGAGGTTAATGGCGGCTCTCAAGGAGCTCGACATGGATGTACAACATGCCAGCGTTTCTGTAGTCAACGATTTGATGATCCAACAAGCCACTGTGAAAATGGGTAGCCGAATTTATACGCAGGATCATCTCAGGTTAGCTCTACATTCGAAAGTCGGGGAAAACCGCCAGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005509 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006970 GO:0006972 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009651 GO:0009718 GO:0009719 GO:0009725 GO:0009753 GO:0009812 GO:0009813 GO:0009889 GO:0009891 GO:0009893 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042221 GO:0042440 GO:0042538 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043425 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046148 GO:0046283 GO:0046872 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901576 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

HLH bHLH-MYC_N

Protein Analysis

766

Amino Acids

84.29

Weight (kDa)

5.89

Isoelectric Point (pI)

50.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	164 - 345	1.3e-55	bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH	PF00010	586 - 632	4.6e-12	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	685 - 756	6.9e-08	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003580)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32640
fragaria_vesca	FvH4_1g13600 FvH4_1g13601 FvH4_4g17280 FvH4_5g22100 FvH4_6g24370 FvH4_6g25910
prunus_persica	Prupe.5G035400_v2.0.a1
pyrus_communis	pycom06g02850 pycom16g24430
rosa_chinensis	RchiOBHm_Chr7g0209751
rosa_laevigata	RLG00000003096 RLG00000008856 RLG00000036489 RLG00000036838
rosa_multiflora	Rmu_sc0000161.1_g000024 Rmu_sc0002616.1_g000020
rosa_roxburghii	Rroxscaffold_1G00006980
rosa_rugosa	Rorug05G0432900 Rorug07G0116000
rosa_samantha	Rh4DG124100 Rh5AG516700 Rh5AG518900 Rh5AG519400 Rh5BG540600 Rh5CG563400 Rh5DG551100 Rh5DG551400 Rh5DG551800 Rh7AG249600 Rh7BG243500 Rh7CG265600 Rh7DG255400
rosa_wichuraiana	Rw0G022750 Rw7G021160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	743
AatII	GACGTC	1 cut(s)	326
Acc36I	ACCTGC	1 cut(s)	373
AccB1I	GGYRCC	3 cut(s)	719, 1079, 1625
AccB7I	CCANNNNNTGG	1 cut(s)	552
AccBSI	CCGCTC	4 cut(s)	901, 1074, 1620, 1774
AccI	GTMKAC	1 cut(s)	1136
AccII	CGCG	7 cut(s)	284, 524, 526, 528, 907, 1543, 2123
AclI	AACGTT	1 cut(s)	1833
AclWI	GGATC	6 cut(s)	324, 759, 985, 1124, 2194, 2253
AcoI	YGGCCR	1 cut(s)	2118
AcsI	RAATTY	5 cut(s)	139, 1375, 1426, 2091, 2232
AcuI	CTGAAG	1 cut(s)	62
AcvI	CACGTG	1 cut(s)	1762
AcyI	GRCGYC	5 cut(s)	323, 720, 741, 1080, 2079
AfaI	GTAC	4 cut(s)	677, 1278, 1299, 2163
AfiI	CCNNNNNNNGG	8 cut(s)	552, 858, 859, 883, 1073, 1412, 1544, 1736
AflIII	ACRYGT	2 cut(s)	282, 726
AgeI	ACCGGT	1 cut(s)	1162
AgsI	TTSAA	8 cut(s)	463, 1042, 1209, 1274, 1402, 1426, 1900, 1998
AhdI	GACNNNNNGTC	3 cut(s)	283, 1111, 1852
AjiI	CACGTC	1 cut(s)	729
AjnI	CCWGG	4 cut(s)	465, 480, 504, 915
AjuI	GAANNNNNNNTTGG	2 cut(s)	124, 156
Alw21I	GWGCWC	7 cut(s)	311, 990, 1241, 1341, 1451, 1893, 2151
Alw26I	GTCTC	3 cut(s)	479, 690, 1910
AlwI	GGATC	6 cut(s)	324, 759, 985, 1124, 2194, 2253
AlwNI	CAGNNNCTG	1 cut(s)	511
Ama87I	CYCGRG	3 cut(s)	1234, 1261, 1477
AoxI	GGCC	8 cut(s)	345, 396, 507, 636, 642, 834, 913, 2118
ApoI	RAATTY	5 cut(s)	139, 1375, 1426, 2091, 2232
AsiGI	ACCGGT	1 cut(s)	1162
Asp700I	GAANNNNTTC	1 cut(s)	1472
AspLEI	GCGC	9 cut(s)	70, 501, 524, 526, 528, 722, 907, 1082, 1460
AspS9I	GGNCC	7 cut(s)	346, 508, 537, 869, 874, 913, 1645
AsuC2I	CCSGG	4 cut(s)	377, 828, 1697, 2117
AsuHPI	GGTGA	2 cut(s)	1177, 1321
AsuII	TTCGAA	3 cut(s)	1838, 2089, 2270
AvaI	CYCGRG	3 cut(s)	1234, 1261, 1477
AvaII	GGWCC	4 cut(s)	537, 869, 874, 1645
BaeI	ACNNNNGTAYC	2 cut(s)	659, 692
BanI	GGYRCC	3 cut(s)	719, 1079, 1625
BanII	GRGCYC	8 cut(s)	311, 990, 1241, 1268, 1341, 1451, 1893, 2151
BauI	CACGAG	1 cut(s)	63
BbrPI	CACGTG	1 cut(s)	1762
BbsI	GAAGAC	1 cut(s)	1980
Bbv12I	GWGCWC	7 cut(s)	311, 990, 1241, 1341, 1451, 1893, 2151
BccI	CCATC	5 cut(s)	216, 286, 554, 588, 1546
BceAI	ACGGC	5 cut(s)	569, 728, 829, 969, 1579
BciT130I	CCWGG	4 cut(s)	467, 482, 506, 917
BciVI	GTATCC	1 cut(s)	240
BcnI	CCSGG	4 cut(s)	377, 828, 1697, 2117
BcoDI	GTCTC	3 cut(s)	479, 690, 1910
BfaI	CTAG	1 cut(s)	1710
BfmI	CTRYAG	3 cut(s)	929, 1904, 2181
BfoI	RGCGCY	4 cut(s)	502, 723, 1083, 1461
BfuAI	ACCTGC	1 cut(s)	373
BfuI	GTATCC	1 cut(s)	240
Bme1390I	CCNGG	8 cut(s)	377, 467, 482, 506, 828, 917, 1697, 2117
Bme18I	GGWCC	4 cut(s)	537, 869, 874, 1645
BmeRI	GACNNNNNGTC	3 cut(s)	283, 1111, 1852
BmeT110I	CYCGRG	3 cut(s)	1234, 1261, 1477
BmgBI	CACGTC	1 cut(s)	729
BmgT120I	GGNCC	7 cut(s)	346, 508, 537, 869, 874, 913, 1645
BmiI	GGNNCC	6 cut(s)	339, 580, 721, 1081, 1161, 1627
BmrFI	CCNGG	8 cut(s)	377, 467, 482, 506, 828, 917, 1697, 2117
BmsI	GCATC	2 cut(s)	462, 1858
BpiI	GAAGAC	1 cut(s)	1980
BpmI	CTGGAG	2 cut(s)	488, 1225
Bpu14I	TTCGAA	3 cut(s)	1838, 2089, 2270
BpuEI	CTTGAG	2 cut(s)	1877, 2126
BpuMI	CCSGG	4 cut(s)	377, 828, 1697, 2117
BsaAI	YACGTR	1 cut(s)	1762
BsaBI	GATNNNNATC	1 cut(s)	1635
BsaHI	GRCGYC	5 cut(s)	323, 720, 741, 1080, 2079
BsaI	GGTCTC	1 cut(s)	690
BsaJI	CCNNGG	7 cut(s)	12, 375, 393, 639, 1146, 1332, 1503
BsaWI	WCCGGW	3 cut(s)	243, 871, 1162
BsaXI	ACNNNNNCTCC	8 cut(s)	557, 563, 587, 593, 689, 719, 1223, 1253
Bsc4I	CCNNNNNNNGG	8 cut(s)	552, 858, 859, 883, 1073, 1412, 1544, 1736
Bse118I	RCCGGY	5 cut(s)	343, 876, 1162, 1213, 1729
Bse1I	ACTGG	1 cut(s)	1242
Bse3DI	GCAATG	1 cut(s)	54
Bse8I	GATNNNNATC	1 cut(s)	1635
BseBI	CCWGG	4 cut(s)	467, 482, 506, 917
BseDI	CCNNGG	7 cut(s)	12, 375, 393, 639, 1146, 1332, 1503
BseGI	GGATG	4 cut(s)	208, 599, 656, 2164
BseJI	GATNNNNATC	1 cut(s)	1635
BseLI	CCNNNNNNNGG	8 cut(s)	552, 858, 859, 883, 1073, 1412, 1544, 1736
BseMI	GCAATG	1 cut(s)	54
BseMII	CTCAG	3 cut(s)	762, 812, 2266
BseNI	ACTGG	1 cut(s)	1242
BsePI	GCGCGC	2 cut(s)	522, 524
BseRI	GAGGAG	3 cut(s)	1459, 1943, 2024
BseX3I	CGGCCG	1 cut(s)	2118
BseYI	CCCAGC	1 cut(s)	348
BsgI	GTGCAG	1 cut(s)	1238
Bsh1236I	CGCG	7 cut(s)	284, 524, 526, 528, 907, 1543, 2123
Bsh1285I	CGRYCG	2 cut(s)	984, 2121
BshFI	GGCC	8 cut(s)	347, 398, 509, 638, 644, 836, 915, 2120
BshNI	GGYRCC	3 cut(s)	719, 1079, 1625
BshTI	ACCGGT	1 cut(s)	1162
BsiEI	CGRYCG	2 cut(s)	984, 2121
BsiHKAI	GWGCWC	7 cut(s)	311, 990, 1241, 1341, 1451, 1893, 2151
BsiHKCI	CYCGRG	3 cut(s)	1234, 1261, 1477
BslFI	GGGAC	3 cut(s)	638, 1811, 2090
BslI	CCNNNNNNNGG	8 cut(s)	552, 858, 859, 883, 1073, 1412, 1544, 1736
BsmAI	GTCTC	3 cut(s)	479, 690, 1910
BsmBI	CGTCTC	2 cut(s)	479, 1910
BsmFI	GGGAC	3 cut(s)	638, 1811, 2090
BsmI	GAATGC	2 cut(s)	1393, 1483
BsnI	GGCC	8 cut(s)	347, 398, 509, 638, 644, 836, 915, 2120
Bso31I	GGTCTC	1 cut(s)	690
BsoBI	CYCGRG	3 cut(s)	1234, 1261, 1477
Bsp119I	TTCGAA	3 cut(s)	1838, 2089, 2270
Bsp1286I	GDGCHC	8 cut(s)	311, 990, 1241, 1268, 1341, 1451, 1893, 2151
Bsp1407I	TGTACA	1 cut(s)	2161
Bsp19I	CCATGG	3 cut(s)	12, 1146, 1332
BspANI	GGCC	8 cut(s)	347, 398, 509, 638, 644, 836, 915, 2120
BspCNI	CTCAG	3 cut(s)	763, 811, 2265
BspFNI	CGCG	7 cut(s)	284, 524, 526, 528, 907, 1543, 2123
BspLI	GGNNCC	6 cut(s)	339, 580, 721, 1081, 1161, 1627
BspMAI	CTGCAG	2 cut(s)	933, 1908
BspMI	ACCTGC	1 cut(s)	373
BspPI	GGATC	6 cut(s)	324, 759, 985, 1124, 2194, 2253
BspQI	GCTCTTC	1 cut(s)	1740
BspT104I	TTCGAA	3 cut(s)	1838, 2089, 2270
BspT107I	GGYRCC	3 cut(s)	719, 1079, 1625
BspTNI	GGTCTC	1 cut(s)	690
BsrBI	CCGCTC	4 cut(s)	901, 1074, 1620, 1774
BsrDI	GCAATG	1 cut(s)	54
BsrFI	RCCGGY	5 cut(s)	343, 876, 1162, 1213, 1729
BsrGI	TGTACA	1 cut(s)	2161
BsrI	ACTGG	1 cut(s)	1242
BssAI	RCCGGY	5 cut(s)	343, 876, 1162, 1213, 1729
BssECI	CCNNGG	7 cut(s)	12, 375, 393, 639, 1146, 1332, 1503
BssHII	GCGCGC	2 cut(s)	522, 524
BssNI	GRCGYC	5 cut(s)	323, 720, 741, 1080, 2079
BssSI	CACGAG	1 cut(s)	63
BssT1I	CCWWGG	4 cut(s)	12, 639, 1146, 1332
Bst2BI	CACGAG	1 cut(s)	63
Bst2UI	CCWGG	4 cut(s)	467, 482, 506, 917
Bst4CI	ACNGT	2 cut(s)	1912, 2215
Bst6I	CTCTTC	4 cut(s)	1520, 1735, 1740, 1777
BstACI	GRCGYC	5 cut(s)	323, 720, 741, 1080, 2079
BstAUI	TGTACA	1 cut(s)	2161
BstBAI	YACGTR	1 cut(s)	1762
BstBI	TTCGAA	3 cut(s)	1838, 2089, 2270
BstC8I	GCNNGC	8 cut(s)	345, 524, 526, 821, 882, 899, 903, 2173
BstDEI	CTNAG	3 cut(s)	771, 798, 2252
BstDSI	CCRYGG	3 cut(s)	12, 1146, 1332
BstF5I	GGATG	4 cut(s)	208, 599, 656, 2164
BstFNI	CGCG	7 cut(s)	284, 524, 526, 528, 907, 1543, 2123
BstH2I	RGCGCY	4 cut(s)	502, 723, 1083, 1461
BstHHI	GCGC	9 cut(s)	70, 501, 524, 526, 528, 722, 907, 1082, 1460
BstMAI	GTCTC	3 cut(s)	479, 690, 1910
BstMCI	CGRYCG	2 cut(s)	984, 2121
BstNI	CCWGG	4 cut(s)	467, 482, 506, 917
BstNSI	RCATGY	1 cut(s)	2171
BstSCI	CCNGG	8 cut(s)	375, 465, 480, 504, 826, 915, 1695, 2115
BstSFI	CTRYAG	3 cut(s)	929, 1904, 2181
BstUI	CGCG	7 cut(s)	284, 524, 526, 528, 907, 1543, 2123
BstV2I	GAAGAC	1 cut(s)	1980
BstX2I	RGATCY	1 cut(s)	316
BstXI	CCANNNNNNTGG	2 cut(s)	1147, 1606
BstYI	RGATCY	1 cut(s)	316
BstZI	CGGCCG	1 cut(s)	2118
BsuI	GTATCC	1 cut(s)	240
BsuRI	GGCC	8 cut(s)	347, 398, 509, 638, 644, 836, 915, 2120
BtgI	CCRYGG	3 cut(s)	12, 1146, 1332
BtgZI	GCGATG	1 cut(s)	1524
BtrI	CACGTC	1 cut(s)	729
BtsCI	GGATG	4 cut(s)	208, 599, 656, 2164
BtsIMutI	CAGTG	2 cut(s)	2102, 2211
BveI	ACCTGC	1 cut(s)	373
Cac8I	GCNNGC	8 cut(s)	345, 524, 526, 821, 882, 899, 903, 2173
CaiI	CAGNNNCTG	1 cut(s)	511
CfoI	GCGC	9 cut(s)	70, 501, 524, 526, 528, 722, 907, 1082, 1460
Cfr10I	RCCGGY	5 cut(s)	343, 876, 1162, 1213, 1729
Cfr13I	GGNCC	7 cut(s)	346, 508, 537, 869, 874, 913, 1645
CpoI	CGGWCCG	1 cut(s)	874
CseI	GACGC	7 cut(s)	273, 496, 749, 1324, 1644, 1842, 2087
Csp6I	GTAC	4 cut(s)	676, 1277, 1298, 2162
CspAI	ACCGGT	1 cut(s)	1162
CspI	CGGWCCG	1 cut(s)	874
CviQI	GTAC	4 cut(s)	676, 1277, 1298, 2162
DdeI	CTNAG	3 cut(s)	771, 798, 2252
DinI	GGCGCC	2 cut(s)	721, 1081
DrdI	GACNNNNNNGTC	1 cut(s)	743
DriI	GACNNNNNGTC	3 cut(s)	283, 1111, 1852
DseDI	GACNNNNNNGTC	1 cut(s)	743
EaeI	YGGCCR	1 cut(s)	2118
EagI	CGGCCG	1 cut(s)	2118
Eam1104I	CTCTTC	4 cut(s)	1520, 1735, 1740, 1777
Eam1105I	GACNNNNNGTC	3 cut(s)	283, 1111, 1852
EarI	CTCTTC	4 cut(s)	1520, 1735, 1740, 1777
EciI	GGCGGA	5 cut(s)	330, 571, 649, 1524, 1688
Ecl136II	GAGCTC	7 cut(s)	309, 988, 1239, 1339, 1449, 1891, 2149
EclXI	CGGCCG	1 cut(s)	2118
Eco130I	CCWWGG	4 cut(s)	12, 639, 1146, 1332
Eco147I	AGGCCT	1 cut(s)	836
Eco24I	GRGCYC	8 cut(s)	311, 990, 1241, 1268, 1341, 1451, 1893, 2151
Eco31I	GGTCTC	1 cut(s)	690
Eco47I	GGWCC	4 cut(s)	537, 869, 874, 1645
Eco52I	CGGCCG	1 cut(s)	2118
Eco53kI	GAGCTC	7 cut(s)	309, 988, 1239, 1339, 1449, 1891, 2149
Eco57I	CTGAAG	1 cut(s)	62
Eco72I	CACGTG	1 cut(s)	1762
Eco88I	CYCGRG	3 cut(s)	1234, 1261, 1477
EcoICRI	GAGCTC	7 cut(s)	309, 988, 1239, 1339, 1449, 1891, 2149
EcoO109I	RGGNCCY	1 cut(s)	508
EcoRI	GAATTC	2 cut(s)	1375, 2091
EcoRII	CCWGG	4 cut(s)	465, 480, 504, 915
EcoT14I	CCWWGG	4 cut(s)	12, 639, 1146, 1332
EcoT22I	ATGCAT	1 cut(s)	477
EcoT38I	GRGCYC	8 cut(s)	311, 990, 1241, 1268, 1341, 1451, 1893, 2151
EgeI	GGCGCC	2 cut(s)	721, 1081
EheI	GGCGCC	2 cut(s)	721, 1081
ErhI	CCWWGG	4 cut(s)	12, 639, 1146, 1332
Esp3I	CGTCTC	2 cut(s)	479, 1910
FaqI	GGGAC	3 cut(s)	638, 1811, 2090
FauI	CCCGC	5 cut(s)	158, 812, 894, 1536, 2067
FblI	GTMKAC	1 cut(s)	1136
FokI	GGATG	4 cut(s)	195, 606, 643, 2171
FriOI	GRGCYC	8 cut(s)	311, 990, 1241, 1268, 1341, 1451, 1893, 2151
FspBI	CTAG	1 cut(s)	1710
GlaI	GCGC	9 cut(s)	69, 500, 523, 525, 527, 721, 906, 1081, 1459
GsaI	CCCAGC	1 cut(s)	352
GsuI	CTGGAG	2 cut(s)	488, 1225
HaeII	RGCGCY	4 cut(s)	502, 723, 1083, 1461
HaeIII	GGCC	8 cut(s)	347, 398, 509, 638, 644, 836, 915, 2120
HgaI	GACGC	7 cut(s)	273, 496, 749, 1324, 1644, 1842, 2087
HhaI	GCGC	9 cut(s)	70, 501, 524, 526, 528, 722, 907, 1082, 1460
Hin1I	GRCGYC	5 cut(s)	323, 720, 741, 1080, 2079
Hin6I	GCGC	9 cut(s)	68, 499, 522, 524, 526, 720, 905, 1080, 1458
HinP1I	GCGC	9 cut(s)	68, 499, 522, 524, 526, 720, 905, 1080, 1458
HincII	GTYRAC	4 cut(s)	811, 1137, 1693, 2188
HindII	GTYRAC	4 cut(s)	811, 1137, 1693, 2188
HindIII	AAGCTT	1 cut(s)	1790
HinfI	GANTC	9 cut(s)	262, 796, 1409, 1631, 1756, 1803, 1949, 1979, 2086
HphI	GGTGA	2 cut(s)	1177, 1321
Hpy166II	GTNNAC	5 cut(s)	270, 811, 1137, 1693, 2188
Hpy188III	TCNNGA	3 cut(s)	755, 1581, 1667
Hpy8I	GTNNAC	5 cut(s)	270, 811, 1137, 1693, 2188
HpyAV	CCTTC	6 cut(s)	175, 469, 512, 596, 1119, 1150
HpyCH4III	ACNGT	2 cut(s)	1912, 2215
HpyCH4IV	ACGT	4 cut(s)	323, 728, 1761, 1833
HpyCH4V	TGCA	8 cut(s)	90, 475, 496, 931, 1219, 1288, 1906, 1974
HpyF3I	CTNAG	3 cut(s)	771, 798, 2252
HpySE526I	ACGT	4 cut(s)	323, 728, 1761, 1833
Hsp92I	GRCGYC	5 cut(s)	323, 720, 741, 1080, 2079
HspAI	GCGC	9 cut(s)	68, 499, 522, 524, 526, 720, 905, 1080, 1458
KasI	GGCGCC	2 cut(s)	719, 1079
KroI	GCCGGC	1 cut(s)	343
KroNI	GCCGGC	1 cut(s)	345
LguI	GCTCTTC	1 cut(s)	1740
LmnI	GCTCC	7 cut(s)	343, 584, 1244, 1336, 1362, 1446, 2146
LweI	GCATC	2 cut(s)	462, 1858
MaeI	CTAG	1 cut(s)	1710
MaeII	ACGT	4 cut(s)	323, 728, 1761, 1833
MaeIII	GTNAC	3 cut(s)	451, 760, 1932
MauBI	CGCGCGCG	1 cut(s)	524
MbiI	CCGCTC	4 cut(s)	901, 1074, 1620, 1774
MfeI	CAATTG	2 cut(s)	1283, 1943
MflI	RGATCY	1 cut(s)	316
MhlI	GDGCHC	8 cut(s)	311, 990, 1241, 1268, 1341, 1451, 1893, 2151
MluI	ACGCGT	1 cut(s)	282
Mly113I	GGCGCC	2 cut(s)	720, 1080
MlyI	GAGTC	2 cut(s)	790, 1958
MmeI	TCCRAC	3 cut(s)	974, 1703, 2227
Mph1103I	ATGCAT	1 cut(s)	477
MroNI	GCCGGC	1 cut(s)	343
MroXI	GAANNNNTTC	1 cut(s)	1472
MseI	TTAA	3 cut(s)	633, 1794, 2129
MslI	CAYNNNNRTG	3 cut(s)	1145, 1151, 2057
MspA1I	CMGCKG	2 cut(s)	1602, 1753
MspR9I	CCNGG	8 cut(s)	377, 467, 482, 506, 828, 917, 1697, 2117
MunI	CAATTG	2 cut(s)	1283, 1943
Mva1269I	GAATGC	2 cut(s)	1393, 1483
MvaI	CCWGG	4 cut(s)	467, 482, 506, 917
MvnI	CGCG	7 cut(s)	284, 524, 526, 528, 907, 1543, 2123
NaeI	GCCGGC	1 cut(s)	345
NarI	GGCGCC	2 cut(s)	720, 1080
NciI	CCSGG	4 cut(s)	377, 828, 1697, 2117
NcoI	CCATGG	3 cut(s)	12, 1146, 1332
NgoMIV	GCCGGC	1 cut(s)	343
NlaIV	GGNNCC	6 cut(s)	339, 580, 721, 1081, 1161, 1627
NmeAIII	GCCGAG	3 cut(s)	374, 1528, 1709
NmuCI	GTSAC	2 cut(s)	451, 760
NsiI	ATGCAT	1 cut(s)	477
NspI	RCATGY	1 cut(s)	2171
NspV	TTCGAA	3 cut(s)	1838, 2089, 2270
PauI	GCGCGC	2 cut(s)	522, 524
PceI	AGGCCT	1 cut(s)	836
PciSI	GCTCTTC	1 cut(s)	1740
PcsI	WCGNNNNNNNCGW	3 cut(s)	320, 744, 1365
PctI	GAATGC	2 cut(s)	1393, 1483
PdiI	GCCGGC	1 cut(s)	345
PdmI	GAANNNNTTC	1 cut(s)	1472
PfeI	GAWTC	7 cut(s)	262, 1409, 1631, 1756, 1803, 1979, 2086
PflFI	GACNNNGTC	2 cut(s)	743, 799
PflMI	CCANNNNNTGG	1 cut(s)	552
PinAI	ACCGGT	1 cut(s)	1162
PleI	GAGTC	2 cut(s)	790, 1957
PluTI	GGCGCC	2 cut(s)	723, 1083
PmaCI	CACGTG	1 cut(s)	1762
PmlI	CACGTG	1 cut(s)	1762
PpsI	GAGTC	2 cut(s)	790, 1957
Ppu21I	YACGTR	1 cut(s)	1762
Psp124BI	GAGCTC	7 cut(s)	311, 990, 1241, 1341, 1451, 1893, 2151
Psp1406I	AACGTT	1 cut(s)	1833
Psp6I	CCWGG	4 cut(s)	465, 480, 504, 915
PspCI	CACGTG	1 cut(s)	1762
PspFI	CCCAGC	1 cut(s)	348
PspGI	CCWGG	4 cut(s)	465, 480, 504, 915
PspN4I	GGNNCC	6 cut(s)	339, 580, 721, 1081, 1161, 1627
PspPI	GGNCC	7 cut(s)	346, 508, 537, 869, 874, 913, 1645
PstI	CTGCAG	2 cut(s)	933, 1908
PstNI	CAGNNNCTG	1 cut(s)	511
PsuI	RGATCY	1 cut(s)	316
PsyI	GACNNNGTC	2 cut(s)	743, 799
PteI	GCGCGC	2 cut(s)	522, 524
PvuII	CAGCTG	1 cut(s)	1602
RsaI	GTAC	4 cut(s)	677, 1278, 1299, 2163
RsaNI	GTAC	4 cut(s)	676, 1277, 1298, 2162
RseI	CAYNNNNRTG	3 cut(s)	1145, 1151, 2057
Rsr2I	CGGWCCG	1 cut(s)	874
RsrII	CGGWCCG	1 cut(s)	874
SacI	GAGCTC	7 cut(s)	311, 990, 1241, 1341, 1451, 1893, 2151
SalI	GTCGAC	1 cut(s)	1135
SapI	GCTCTTC	1 cut(s)	1740
SaqAI	TTAA	3 cut(s)	633, 1794, 2129
Sau96I	GGNCC	7 cut(s)	346, 508, 537, 869, 874, 913, 1645
SchI	GAGTC	2 cut(s)	790, 1958
ScrFI	CCNGG	8 cut(s)	377, 467, 482, 506, 828, 917, 1697, 2117
SduI	GDGCHC	8 cut(s)	311, 990, 1241, 1268, 1341, 1451, 1893, 2151
SfaNI	GCATC	2 cut(s)	462, 1858
SfcI	CTRYAG	3 cut(s)	929, 1904, 2181
SfoI	GGCGCC	2 cut(s)	721, 1081
SfuI	TTCGAA	3 cut(s)	1838, 2089, 2270
SinI	GGWCC	4 cut(s)	537, 869, 874, 1645
SmiMI	CAYNNNNRTG	3 cut(s)	1145, 1151, 2057
SmlI	CTYRAG	2 cut(s)	1892, 2141
SmoI	CTYRAG	2 cut(s)	1892, 2141
SseBI	AGGCCT	1 cut(s)	836
SspDI	GGCGCC	2 cut(s)	719, 1079
SspMI	CTAG	1 cut(s)	1710
SstI	GAGCTC	7 cut(s)	311, 990, 1241, 1341, 1451, 1893, 2151
StuI	AGGCCT	1 cut(s)	836
StyD4I	CCNGG	8 cut(s)	375, 465, 480, 504, 826, 915, 1695, 2115
StyI	CCWWGG	4 cut(s)	12, 639, 1146, 1332
TaaI	ACNGT	2 cut(s)	1912, 2215
TaiI	ACGT	4 cut(s)	326, 731, 1764, 1836
TaqII	GACCGA	1 cut(s)	998
TatI	WGTACW	2 cut(s)	1297, 2161
TfiI	GAWTC	7 cut(s)	262, 1409, 1631, 1756, 1803, 1979, 2086
Tru1I	TTAA	3 cut(s)	633, 1794, 2129
Tru9I	TTAA	3 cut(s)	633, 1794, 2129
TscAI	CASTG	2 cut(s)	2102, 2218
TseFI	GTSAC	2 cut(s)	451, 760
Tsp45I	GTSAC	2 cut(s)	451, 760
TspDTI	ATGAA	2 cut(s)	1028, 1970
TspGWI	ACGGA	6 cut(s)	251, 287, 439, 882, 1122, 1934
TspRI	CASTG	2 cut(s)	2102, 2218
Tth111I	GACNNNGTC	2 cut(s)	743, 799
Van91I	CCANNNNNTGG	1 cut(s)	552
VpaK11BI	GGWCC	4 cut(s)	537, 869, 874, 1645
XapI	RAATTY	5 cut(s)	139, 1375, 1426, 2091, 2232
XceI	RCATGY	1 cut(s)	2171
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2218
XmiI	GTMKAC	1 cut(s)	1136
XmnI	GAANNNNTTC	1 cut(s)	1472
XspI	CTAG	1 cut(s)	1710
ZraI	GACGTC	1 cut(s)	324
Zsp2I	ATGCAT	1 cut(s)	477

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.