Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0006735

Physical Location & Seq

Genomic Coordinates Reverse (-)

23354994 .. 23358874

Gene Structure 3881 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3402 bp

ATGGAAGACCCTCTCGTGTATTGGATAGGTGATGAACAAAATGTAGTTCAAACCCAATGTTTAGAGGCTTCGGACTTAATTTTCCAAGAGAAACAATCCACAGTTCCTTTGCAATTTGTATTCTTGTTGTCCCCCTTCTTGCATGCTTTCCAACCAAGAAGATCTCAACAACATATATTTGCACAAGCATGCAAACACACCATGTTCCTCACAAGCATTCCCTCATCTAAAACCCTAATCACCCCAAAAACCCTAACCCTACCTCTCGCATTCCATTTCACAACCTCACCACCTTCCGATCAACAACCACCGCAACACCTCCACCAAGACCAAGCAATCAGCATAGAAGACAACAGATCATACTTCACCAAAAAAATCCACAGCCTCTGCGCCACCCACCGCGACGTCGACGAAGCCCTCCGCCTCCTCGATCGCCTCCGCCTTCACGGCTACCGTCCCGACTCCCTCAACCTCAGCAGCGTCGTCCACGCTCTCTGCGACTCCAACCGCTTCGATGAAGCCCACCACCGCTTTGGCCACTATGTAGCTTCCGACTGCGCCCCCGACGAGCGCACTTGCAATGTTATTGTTGCTCGGTTGCTGGATTCAAAAACCCCCCATGCCACATTGCGTGTTCTGCGCTGTTTGACAAGGTTTAAGCCCGATTTCGTGGCGTCTTTGGTTAATTACAACCGTTTGATCGACCAATTTTGCTCGATCTTACGACCCAATGAGGGTCATAGAGTGTTGTTTGATATGATTAGTAGAGGGCATTGTCCGAATGCTGTTTCTTACACTACATTGATCAATGGGTATTGTTTGATTGGTGCGTTGGGTGATGCGCAGAAGGTGTTCGATGAAATGAGTGAAAGGGGAGTGGCGCCTAATTCCCTGACGCATAGCGTGATGATTCGTGGTGTTCTTCGGCAGAGGGATATTGGGCGTGCAAAAGAATTGATGGGAAAGCTCTGGGAGATCATGAAACGTGAAGATGATGAGACTGTAAAGACTGCGGCTTTTGGGAATCTCATTGATTCGATGTGTGGAGAAGGGTTTTTTAATGAAGTGTTTGACATTGCCGAAGATATGCCACAGGGGAGTGTGACAGAGGATTTTGCTTATGGACAAATGATAGATTCGCTTTGTAAAGCCAGAAGGCATCATGGGGCGTCCAGGATTGTTTATATTATGAGGAGTAGAGGGTTTGTTCCCAAGCTAACGTCGTATAATTCCATTTTGCATGGACTGAGCAAGGAGGGAGAGGGAGGTTGCATGAGGGCTTATCAGTTGCTAGAGGAAGGAGGGAACTTTGGTTACTTTCCGTCAGAGCACACTTACAAGGTCCTATTGGAAGGTCTGTGCCAAGAATCGGACGTCAAGAAGGCAAGAGAGGTTCTTGAATATATGTTAAAGAAGGAAGGAGTGGACAGAACTAGGATTTATAACATATATTTAAGAGCGTTGTGTCTTGTGAATAACACTACTGAGCTTTTTAATGGACTTGTTTCGATGCTCCAGAGCCAGTGTCAGCCTGATGTGATCACCCTAAACACAGTTCTTAATGGGTTCTGCAAGATGGAAAGAATCGAGGAAGCGCTAAAGGTACTAGATGACATGATGAGTGGCAAGTTTTGTGCACCAGACGTGGTGACCTTCACAACGATCATCAGTGGTTTAATAAATGTGGGAAGAACTCAAGAAGCTCTTGTTATGCTGCACCATGTAATGCCCGAGAAAGGTTTCAGAGCTAATGTTGTGACGTACAATGCTGTTCTTCATGGTTTATTCAAGCATAGGCAAGCGAGAGAAGCAATGGATTTGTTCAATGGCATGGCAAATGACGGCGTGGCCGCTGACAGTACTACTTATACTATAATAATTGACGGGTTATGCGAATCAGATCAAATAGAAGAAGCTAAGAAGTTTTGGGATGAGGTTATTTGGCCATCAAAGATCCACGATAATTTTGTTTATGCTGCCCTTATCAAAGGGATTTGTCGTTCTGGGAGGTTTGAAGAAGCGTGCCATTTCCTTTATGAACTAGTAGATGCTGGGGTCTCTCCAAATATATACAGTTATAACATTGTGATTGATGCTGCTTGCAAATCAGGATTGAGGAAGGAAGCGTATGAAGTTGTAAGAGAGATGAAAAGAAACGGACTGGCTCCAGATTCTGTAACATGGAGGATACTTGACAAGTTGCAGGGTGTCAGAAAACAATCTTGTGATGAGGTTTCGAATTTTCAATCAATAAATGGGCGACAGGGGGATGTTGGGCGTGCAAAAGAATTGATGGGAAAGCTCTGGGAGATCATGAAAGGTGAAGATGATGCGACTGTAAAGAGTGCGGCCTTTGGGAATCTCATTGATTCTATGTGTAGAGAAGGGTTTTTTAATGAAGTGTTTGACATTGCCGAAGATATGCCACAGGGGAAGAGTGTGACAGAGGATTTTGCTTATGGACAAATGATAGATTCGCTTTGTAAAGCCAGAAGGCTTCATGGGGCGTCCAGGATTGTTTATATTATGAGGAGTAGAGGGTTTGTTCCAAAGCCAACGTCGTATACTTCCATTTTGCATGGACTAAGCAAGGAGGGAGAGGGAGGTTGCATGAGGGCTTATCAGTTGCTAGAGGAAGGAGTGAACTTTGGTTACTTTCCGTCAGAGTACACTTACAAGGTCCTATTGGAAGGTCTGTGCCAAGAATCGGACGTCAAGAAGGCAAGAGAGGTTCTTGAATATATGTTAAAGAAGGAAGGAGTGGACAGAACTAGGATTTATAACATATATTTGAGAACGTTGTGTCTTGTGAATAACACGACTGAGCTTTTGAATGGACTTGTTTCGATGCTCCAGAGCCAGTGTCAGCCTGATGTGATCATCCTAAACACAGTTCTTAATGGGTTCTGCAAGATGGAAAGAATCAAGGAAGCGCTAAAGGTACTAGATGACATGATGAGTGGCAAGTTTTGTGCACCAGACGTGGTGACCTTCACAACGATCATCAGTGGTTTAATAAATGTGGGAAGAACTCAAGAAGCTCTTGTTATGCTGTACCATGTAATGCCCGAGAAAGGTTTCAGAGCTAATGTTGTGACGTACAATGCTGTTCTTCGTGGTTTATTCAAGCATAGGCAAGCGAGAGAAGCAATGGATTTGTTCAATGGCATGGCAAACGACGATGTGGCTGCTGACAGTACTACTTATACTATAATAATTGACGGGTTATGTGAATCAGATCAAATTGAAGAAGCTAAGAAGTTTTGGGATGAGGTTATTTGGCCATCAAAAGATCGATCCACGATAATTTTGTTTATGCTGCCCTTATCAAAGGGATTTGTCATTCTGGGAGGTTTGAAGAAGCGTGCCATTTCCTTTATGAACTATCTGTTAGAGAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

1134

Amino Acids

128.13

Weight (kDa)

6.16

Isoelectric Point (pI)

40.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	227 - 273	1.3e-09	PPR repeat family
PPR_3	PF13812	249 - 304	1.2e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	256 - 289	6.5e-12	PPR repeat
PPR_2	PF13041	260 - 307	7e-15	PPR repeat family
PPR	PF01535	263 - 293	9.3e-07	PPR repeat
PPR_2	PF13041	480 - 525	1.6e-06	PPR repeat family
PPR_1	PF12854	508 - 539	1e-10	PPR repeat
PPR_2	PF13041	511 - 560	2e-13	PPR repeat family
PPR_2	PF13041	547 - 595	1.3e-09	PPR repeat family
PPR_3	PF13812	574 - 628	6.2e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	584 - 631	2.7e-16	PPR repeat family
PPR_1	PF12854	615 - 645	4.3e-06	PPR repeat
PPR_2	PF13041	654 - 702	2.4e-11	PPR repeat family
PPR_3	PF13812	679 - 729	3.6e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	685 - 717	4.2e-09	PPR repeat
PPR	PF01535	691 - 721	3.2e-06	PPR repeat
PPR_2	PF13041	691 - 731	1.4e-10	PPR repeat family
PPR_1	PF12854	884 - 915	2.3e-06	PPR repeat
PPR_1	PF12854	954 - 985	2.3e-09	PPR repeat
PPR_2	PF13041	957 - 1006	1.7e-12	PPR repeat family
PPR_2	PF13041	993 - 1035	3.5e-08	PPR repeat family
PPR_2	PF13041	1030 - 1077	4.9e-15	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006735)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18020
fragaria_vesca	FvH4_5g17250
malus_domestica	MD06G1231100.v1.1
prunus_persica	Prupe.5G232400_v2.0.a1
pyrus_communis	pycom06g20240
rosa_chinensis	RchiOBHm_Chr7g0178541
rosa_laevigata	RLG00000005439 RLG00000010173
rosa_multiflora	Rmu_sc0000775.1_g000015 Rmu_sc0000961.1_g000015 Rmu_sc0002421.1_g000004 Rmu_sc0003637.1_g000006 Rmu_sc0011483.1_g000001 Rmu_sc0016060.1_g000005 Rmu_ssc0000343.1_g000033
rosa_roxburghii	Rroxscaffold_3G00274830 Rroxscaffold_5G00333820
rosa_rugosa	Rorug06G0417900
rosa_samantha	Rh3CG367500 Rh4AG426400 Rh4DG434700 Rh7AG016400 Rh7BG015900 Rh7CG016600 Rh7DG016500
rosa_wichuraiana	Rw7G001300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1443, 2079, 2781
AatII	GACGTC	3 cut(s)	408, 1377, 2715
Acc16I	TGCGCA	1 cut(s)	843
AccB1I	GGYRCC	1 cut(s)	880
AccI	GTMKAC	2 cut(s)	408, 2564
AccII	CGCG	1 cut(s)	402
AciI	CCGC	9 cut(s)	311, 400, 421, 439, 508, 529, 1013, 1851, 2348
AclI	AACGTT	1 cut(s)	2798
AclWI	GGATC	2 cut(s)	1948, 3291
AcoI	YGGCCR	4 cut(s)	535, 1848, 1943, 3281
AcsI	RAATTY	1 cut(s)	2239
AcyI	GRCGYC	7 cut(s)	405, 674, 881, 1169, 1374, 2507, 2712
AfaI	GTAC	8 cut(s)	1605, 1763, 1861, 2669, 2943, 3056, 3101, 3199
AfeI	AGCGCT	2 cut(s)	1596, 2934
AfiI	CCNNNNNNNGG	5 cut(s)	734, 1369, 1736, 2707, 3074
AhlI	ACTAGT	1 cut(s)	2041
AjiI	CACGTC	2 cut(s)	1645, 2983
AjnI	CCWGG	2 cut(s)	1172, 2510
AloI	GAACNNNNNNTCC	2 cut(s)	1984, 2016
Alw21I	GWGCWC	3 cut(s)	1332, 1639, 2977
Alw26I	GTCTC	2 cut(s)	992, 2062
Alw44I	GTGCAC	2 cut(s)	1635, 2973
AlwI	GGATC	2 cut(s)	1948, 3291
AlwNI	CAGNNNCTG	2 cut(s)	387, 2174
Ama87I	CYCGRG	2 cut(s)	1730, 3068
Aor51HI	AGCGCT	2 cut(s)	1596, 2934
AoxI	GGCC	5 cut(s)	535, 1848, 1943, 2349, 3281
ApaLI	GTGCAC	2 cut(s)	1635, 2973
ApeKI	GCWGC	6 cut(s)	477, 1714, 1976, 2096, 3188, 3319
ApoI	RAATTY	1 cut(s)	2239
ArsI	GACNNNNNNTTYG	4 cut(s)	2543, 2575, 2812, 2844
Asp700I	GAANNNNTTC	3 cut(s)	851, 1739, 3077
AspLEI	GCGC	8 cut(s)	392, 560, 573, 642, 844, 883, 1597, 2935
AspS9I	GGNCC	2 cut(s)	1342, 2680
AsuHPI	GGTGA	9 cut(s)	41, 232, 279, 358, 848, 1534, 1660, 2333, 2998
AsuII	TTCGAA	1 cut(s)	2237
AvaI	CYCGRG	2 cut(s)	1730, 3068
AvaII	GGWCC	2 cut(s)	1342, 2680
BaeGI	GKGCMC	2 cut(s)	1639, 2977
BalI	TGGCCA	3 cut(s)	537, 1945, 3283
BanI	GGYRCC	1 cut(s)	880
BauI	CACGAG	1 cut(s)	14
BbsI	GAAGAC	2 cut(s)	12, 354
Bbv12I	GWGCWC	3 cut(s)	1332, 1639, 2977
BbvCI	CCTCAGC	1 cut(s)	473
BbvI	GCAGC	6 cut(s)	489, 1701, 1963, 2083, 3175, 3306
BccI	CCATC	6 cut(s)	952, 1570, 1954, 2287, 2908, 3292
BceAI	ACGGC	2 cut(s)	463, 1858
BcgI	CGANNNNNNTGC	2 cut(s)	3170, 3204
BciT130I	CCWGG	2 cut(s)	1174, 2512
BciVI	GTATCC	1 cut(s)	2181
BclI	TGATCA	3 cut(s)	804, 1539, 2877
BcoDI	GTCTC	2 cut(s)	992, 2062
BcuI	ACTAGT	1 cut(s)	2041
BfaI	CTAG	7 cut(s)	1292, 1434, 1607, 2042, 2630, 2772, 2945
BfoI	RGCGCY	3 cut(s)	884, 1598, 2936
BfuI	GTATCC	1 cut(s)	2181
BglI	GCCNNNNNGGC	1 cut(s)	447
BglII	AGATCT	1 cut(s)	161
BisI	GCNGC	9 cut(s)	478, 1014, 1715, 1851, 1977, 2097, 2349, 3189, 3320
BlsI	GCNGC	9 cut(s)	479, 1015, 1716, 1852, 1978, 2098, 2350, 3190, 3321
BmcAI	AGTACT	2 cut(s)	1861, 3199
Bme1390I	CCNGG	2 cut(s)	1174, 2512
Bme18I	GGWCC	2 cut(s)	1342, 2680
BmeT110I	CYCGRG	2 cut(s)	1730, 3068
BmgBI	CACGTC	2 cut(s)	1645, 2983
BmgT120I	GGNCC	2 cut(s)	1342, 2680
BmiI	GGNNCC	2 cut(s)	882, 2166
BmrFI	CCNGG	2 cut(s)	1174, 2512
BmsI	GCATC	7 cut(s)	829, 1168, 1500, 2038, 2083, 2320, 2838
BpiI	GAAGAC	2 cut(s)	12, 354
BpmI	CTGGAG	3 cut(s)	1499, 2151, 2837
Bpu10I	CCTNAGC	1 cut(s)	473
Bpu14I	TTCGAA	1 cut(s)	2237
BpuEI	CTTGAG	2 cut(s)	1680, 3018
Bsa29I	ATCGAT	1 cut(s)	3295
BsaHI	GRCGYC	7 cut(s)	405, 674, 881, 1169, 1374, 2507, 2712
BsaI	GGTCTC	1 cut(s)	2062
Bsc4I	CCNNNNNNNGG	5 cut(s)	734, 1369, 1736, 2707, 3074
Bse1I	ACTGG	3 cut(s)	1522, 2166, 2860
Bse3DI	GCAATG	6 cut(s)	586, 626, 1074, 1818, 2409, 3156
BseBI	CCWGG	2 cut(s)	1174, 2512
BseCI	ATCGAT	1 cut(s)	3295
BseGI	GGATG	4 cut(s)	1936, 2275, 2880, 3274
BseLI	CCNNNNNNNGG	5 cut(s)	734, 1369, 1736, 2707, 3074
BseMI	GCAATG	6 cut(s)	586, 626, 1074, 1818, 2409, 3156
BseMII	CTCAG	4 cut(s)	487, 1238, 1476, 2814
BseNI	ACTGG	3 cut(s)	1522, 2166, 2860
BseRI	GAGGAG	3 cut(s)	416, 1207, 2545
BseSI	GKGCMC	2 cut(s)	1639, 2977
BseXI	GCAGC	6 cut(s)	489, 1701, 1963, 2083, 3175, 3306
BseYI	CCCAGC	1 cut(s)	2051
BsgI	GTGCAG	1 cut(s)	1700
Bsh1236I	CGCG	1 cut(s)	402
Bsh1285I	CGRYCG	1 cut(s)	433
BshFI	GGCC	5 cut(s)	537, 1850, 1945, 2351, 3283
BshNI	GGYRCC	1 cut(s)	880
BshVI	ATCGAT	1 cut(s)	3295
BsiEI	CGRYCG	1 cut(s)	433
BsiHKAI	GWGCWC	3 cut(s)	1332, 1639, 2977
BsiHKCI	CYCGRG	2 cut(s)	1730, 3068
BslFI	GGGAC	2 cut(s)	115, 441
BslI	CCNNNNNNNGG	5 cut(s)	734, 1369, 1736, 2707, 3074
BsmAI	GTCTC	2 cut(s)	992, 2062
BsmFI	GGGAC	2 cut(s)	115, 441
BsmI	GAATGC	3 cut(s)	216, 269, 787
BsnI	GGCC	5 cut(s)	537, 1850, 1945, 2351, 3283
Bso31I	GGTCTC	1 cut(s)	2062
BsoBI	CYCGRG	2 cut(s)	1730, 3068
Bsp119I	TTCGAA	1 cut(s)	2237
Bsp1286I	GDGCHC	3 cut(s)	1332, 1639, 2977
BspACI	CCGC	9 cut(s)	311, 400, 421, 439, 508, 529, 1013, 1851, 2348
BspANI	GGCC	5 cut(s)	537, 1850, 1945, 2351, 3283
BspCNI	CTCAG	4 cut(s)	486, 1239, 1477, 2815
BspDI	ATCGAT	1 cut(s)	3295
BspFNI	CGCG	1 cut(s)	402
BspHI	TCATGA	2 cut(s)	978, 2313
BspLI	GGNNCC	2 cut(s)	882, 2166
BspPI	GGATC	2 cut(s)	1948, 3291
BspT104I	TTCGAA	1 cut(s)	2237
BspT107I	GGYRCC	1 cut(s)	880
BspTNI	GGTCTC	1 cut(s)	2062
BsrDI	GCAATG	6 cut(s)	586, 626, 1074, 1818, 2409, 3156
BsrI	ACTGG	3 cut(s)	1522, 2166, 2860
BssNAI	GTATAC	1 cut(s)	2565
BssNI	GRCGYC	7 cut(s)	405, 674, 881, 1169, 1374, 2507, 2712
BssSI	CACGAG	1 cut(s)	14
Bst1107I	GTATAC	1 cut(s)	2565
Bst2BI	CACGAG	1 cut(s)	14
Bst2UI	CCWGG	2 cut(s)	1174, 2512
Bst6I	CTCTTC	1 cut(s)	2429
BstACI	GRCGYC	7 cut(s)	405, 674, 881, 1169, 1374, 2507, 2712
BstBI	TTCGAA	1 cut(s)	2237
BstC8I	GCNNGC	9 cut(s)	144, 190, 945, 1800, 2023, 2101, 2280, 3138, 3366
BstDEI	CTNAG	7 cut(s)	473, 1247, 1485, 1917, 2585, 2823, 3255
BstEII	GGTNACC	2 cut(s)	1648, 2986
BstF5I	GGATG	4 cut(s)	1936, 2275, 2880, 3274
BstFNI	CGCG	1 cut(s)	402
BstH2I	RGCGCY	3 cut(s)	884, 1598, 2936
BstHHI	GCGC	8 cut(s)	392, 560, 573, 642, 844, 883, 1597, 2935
BstMAI	GTCTC	2 cut(s)	992, 2062
BstMCI	CGRYCG	1 cut(s)	433
BstMWI	GCNNNNNNNGC	4 cut(s)	447, 637, 1808, 3146
BstNI	CCWGG	2 cut(s)	1174, 2512
BstNSI	RCATGY	2 cut(s)	146, 192
BstPI	GGTNACC	2 cut(s)	1648, 2986
BstSCI	CCNGG	2 cut(s)	1172, 2510
BstSLI	GKGCMC	2 cut(s)	1639, 2977
BstUI	CGCG	1 cut(s)	402
BstV1I	GCAGC	6 cut(s)	489, 1701, 1963, 2083, 3175, 3306
BstV2I	GAAGAC	2 cut(s)	12, 354
BstX2I	RGATCY	2 cut(s)	161, 1953
BstXI	CCANNNNNNTGG	1 cut(s)	533
BstYI	RGATCY	2 cut(s)	161, 1953
BstZ17I	GTATAC	1 cut(s)	2565
Bsu15I	ATCGAT	1 cut(s)	3295
BsuI	GTATCC	1 cut(s)	2181
BsuRI	GGCC	5 cut(s)	537, 1850, 1945, 2351, 3283
BsuTUI	ATCGAT	1 cut(s)	3295
BtrI	CACGTC	2 cut(s)	1645, 2983
BtsCI	GGATG	4 cut(s)	1936, 2275, 2880, 3274
BtsIMutI	CAGTG	4 cut(s)	1529, 1675, 2867, 3013
Cac8I	GCNNGC	9 cut(s)	144, 190, 945, 1800, 2023, 2101, 2280, 3138, 3366
CaiI	CAGNNNCTG	2 cut(s)	387, 2174
CciI	TCATGA	2 cut(s)	978, 2313
CfoI	GCGC	8 cut(s)	392, 560, 573, 642, 844, 883, 1597, 2935
Cfr13I	GGNCC	2 cut(s)	1342, 2680
ClaI	ATCGAT	1 cut(s)	3295
CseI	GACGC	5 cut(s)	469, 663, 904, 1158, 2496
Csp6I	GTAC	8 cut(s)	1604, 1762, 1860, 2668, 2942, 3055, 3100, 3198
CviQI	GTAC	8 cut(s)	1604, 1762, 1860, 2668, 2942, 3055, 3100, 3198
DdeI	CTNAG	7 cut(s)	473, 1247, 1485, 1917, 2585, 2823, 3255
DinI	GGCGCC	1 cut(s)	882
EaeI	YGGCCR	4 cut(s)	535, 1848, 1943, 3281
Eam1104I	CTCTTC	1 cut(s)	2429
EarI	CTCTTC	1 cut(s)	2429
EciI	GGCGGA	2 cut(s)	410, 428
Eco31I	GGTCTC	1 cut(s)	2062
Eco47I	GGWCC	2 cut(s)	1342, 2680
Eco47III	AGCGCT	2 cut(s)	1596, 2934
Eco88I	CYCGRG	2 cut(s)	1730, 3068
Eco91I	GGTNACC	2 cut(s)	1648, 2986
EcoO109I	RGGNCCY	2 cut(s)	1342, 2680
EcoO65I	GGTNACC	2 cut(s)	1648, 2986
EcoRII	CCWGG	2 cut(s)	1172, 2510
EgeI	GGCGCC	1 cut(s)	882
EheI	GGCGCC	1 cut(s)	882
FalI	AAGNNNNNCTT	4 cut(s)	1689, 1721, 3027, 3059
FaqI	GGGAC	2 cut(s)	115, 441
FbaI	TGATCA	3 cut(s)	804, 1539, 2877
FblI	GTMKAC	2 cut(s)	408, 2564
Fnu4HI	GCNGC	9 cut(s)	478, 1014, 1715, 1851, 1977, 2097, 2349, 3189, 3320
FokI	GGATG	4 cut(s)	1943, 2282, 2867, 3281
Fsp4HI	GCNGC	9 cut(s)	478, 1014, 1715, 1851, 1977, 2097, 2349, 3189, 3320
FspBI	CTAG	7 cut(s)	1292, 1434, 1607, 2042, 2630, 2772, 2945
FspI	TGCGCA	1 cut(s)	843
GlaI	GCGC	8 cut(s)	391, 559, 572, 641, 843, 882, 1596, 2934
GluI	GCNGC	9 cut(s)	478, 1014, 1715, 1851, 1977, 2097, 2349, 3189, 3320
GsaI	CCCAGC	1 cut(s)	2055
GsuI	CTGGAG	3 cut(s)	1499, 2151, 2837
HaeII	RGCGCY	3 cut(s)	884, 1598, 2936
HaeIII	GGCC	5 cut(s)	537, 1850, 1945, 2351, 3283
HgaI	GACGC	5 cut(s)	469, 663, 904, 1158, 2496
HhaI	GCGC	8 cut(s)	392, 560, 573, 642, 844, 883, 1597, 2935
Hin1I	GRCGYC	7 cut(s)	405, 674, 881, 1169, 1374, 2507, 2712
Hin6I	GCGC	8 cut(s)	390, 558, 571, 640, 842, 881, 1595, 2933
HinP1I	GCGC	8 cut(s)	390, 558, 571, 640, 842, 881, 1595, 2933
HincII	GTYRAC	1 cut(s)	409
HindII	GTYRAC	1 cut(s)	409
HphI	GGTGA	9 cut(s)	41, 232, 279, 358, 848, 1534, 1660, 2333, 2998
Hpy166II	GTNNAC	9 cut(s)	409, 487, 1426, 1637, 2565, 2644, 2670, 2764, 2975
Hpy8I	GTNNAC	9 cut(s)	409, 487, 1426, 1637, 2565, 2644, 2670, 2764, 2975
Hpy99I	CGWCG	8 cut(s)	407, 410, 413, 485, 569, 1225, 2563, 3182
HpyF10VI	GCNNNNNNNGC	4 cut(s)	447, 637, 1808, 3146
HpyF3I	CTNAG	7 cut(s)	473, 1247, 1485, 1917, 2585, 2823, 3255
Hsp92I	GRCGYC	7 cut(s)	405, 674, 881, 1169, 1374, 2507, 2712
HspAI	GCGC	8 cut(s)	390, 558, 571, 640, 842, 881, 1595, 2933
KasI	GGCGCC	1 cut(s)	880
Ksp22I	TGATCA	3 cut(s)	804, 1539, 2877
LmnI	GCTCC	3 cut(s)	1518, 2170, 2856
Lsp1109I	GCAGC	6 cut(s)	489, 1701, 1963, 2083, 3175, 3306
LweI	GCATC	7 cut(s)	829, 1168, 1500, 2038, 2083, 2320, 2838
MaeI	CTAG	7 cut(s)	1292, 1434, 1607, 2042, 2630, 2772, 2945
MaeIII	GTNAC	9 cut(s)	1102, 1313, 1648, 1756, 2176, 2440, 2651, 2986, 3094
MflI	RGATCY	2 cut(s)	161, 1953
MhlI	GDGCHC	3 cut(s)	1332, 1639, 2977
MlsI	TGGCCA	3 cut(s)	537, 1945, 3283
MluNI	TGGCCA	3 cut(s)	537, 1945, 3283
Mly113I	GGCGCC	1 cut(s)	881
MlyI	GAGTC	2 cut(s)	455, 494
MmeI	TCCRAC	3 cut(s)	175, 528, 576
Mox20I	TGGCCA	3 cut(s)	537, 1945, 3283
MroXI	GAANNNNTTC	3 cut(s)	851, 1739, 3077
MscI	TGGCCA	3 cut(s)	537, 1945, 3283
MslI	CAYNNNNRTG	1 cut(s)	187
Msp20I	TGGCCA	3 cut(s)	537, 1945, 3283
MspA1I	CMGCKG	1 cut(s)	1853
MspR9I	CCNGG	2 cut(s)	1174, 2512
Mva1269I	GAATGC	3 cut(s)	216, 269, 787
MvaI	CCWGG	2 cut(s)	1174, 2512
MvnI	CGCG	1 cut(s)	402
MwoI	GCNNNNNNNGC	4 cut(s)	447, 637, 1808, 3146
NarI	GGCGCC	1 cut(s)	881
NlaIV	GGNNCC	2 cut(s)	882, 2166
NmuCI	GTSAC	6 cut(s)	1102, 1648, 1756, 2440, 2986, 3094
NsbI	TGCGCA	1 cut(s)	843
NspI	RCATGY	2 cut(s)	146, 192
NspV	TTCGAA	1 cut(s)	2237
PaeI	GCATGC	2 cut(s)	146, 192
PagI	TCATGA	2 cut(s)	978, 2313
PcsI	WCGNNNNNNNCGW	2 cut(s)	495, 1890
PctI	GAATGC	3 cut(s)	216, 269, 787
PdmI	GAANNNNTTC	3 cut(s)	851, 1739, 3077
PfoI	TCCNGGA	2 cut(s)	1172, 2510
PkrI	GCNGC	9 cut(s)	479, 1015, 1716, 1852, 1978, 2098, 2350, 3190, 3321
Ple19I	CGATCG	1 cut(s)	433
PleI	GAGTC	2 cut(s)	455, 494
PluTI	GGCGCC	1 cut(s)	884
PpsI	GAGTC	2 cut(s)	455, 494
PpuMI	RGGWCCY	2 cut(s)	1342, 2680
PsiI	TTATAA	3 cut(s)	1443, 2079, 2781
Psp1406I	AACGTT	1 cut(s)	2798
Psp5II	RGGWCCY	2 cut(s)	1342, 2680
Psp6I	CCWGG	2 cut(s)	1172, 2510
PspEI	GGTNACC	2 cut(s)	1648, 2986
PspFI	CCCAGC	1 cut(s)	2051
PspGI	CCWGG	2 cut(s)	1172, 2510
PspN4I	GGNNCC	2 cut(s)	882, 2166
PspPI	GGNCC	2 cut(s)	1342, 2680
PspPPI	RGGWCCY	2 cut(s)	1342, 2680
PsrI	GAACNNNNNNTAC	4 cut(s)	1298, 1330, 2636, 2668
PstNI	CAGNNNCTG	2 cut(s)	387, 2174
PsuI	RGATCY	2 cut(s)	161, 1953
PvuI	CGATCG	1 cut(s)	433
RsaI	GTAC	8 cut(s)	1605, 1763, 1861, 2669, 2943, 3056, 3101, 3199
RsaNI	GTAC	8 cut(s)	1604, 1762, 1860, 2668, 2942, 3055, 3100, 3198
RseI	CAYNNNNRTG	1 cut(s)	187
SalI	GTCGAC	1 cut(s)	407
SatI	GCNGC	9 cut(s)	478, 1014, 1715, 1851, 1977, 2097, 2349, 3189, 3320
Sau96I	GGNCC	2 cut(s)	1342, 2680
ScaI	AGTACT	2 cut(s)	1861, 3199
SchI	GAGTC	2 cut(s)	455, 494
ScrFI	CCNGG	2 cut(s)	1174, 2512
SduI	GDGCHC	3 cut(s)	1332, 1639, 2977
SfaNI	GCATC	7 cut(s)	829, 1168, 1500, 2038, 2083, 2320, 2838
SfoI	GGCGCC	1 cut(s)	882
SfuI	TTCGAA	1 cut(s)	2237
SgrDI	CGTCGACG	1 cut(s)	407
SinI	GGWCC	2 cut(s)	1342, 2680
SmiMI	CAYNNNNRTG	1 cut(s)	187
SmlI	CTYRAG	2 cut(s)	1695, 3033
SmoI	CTYRAG	2 cut(s)	1695, 3033
SpeI	ACTAGT	1 cut(s)	2041
SphI	GCATGC	2 cut(s)	146, 192
SsiI	CCGC	9 cut(s)	311, 400, 421, 439, 508, 529, 1013, 1851, 2348
SspDI	GGCGCC	1 cut(s)	880
SspMI	CTAG	7 cut(s)	1292, 1434, 1607, 2042, 2630, 2772, 2945
StyD4I	CCNGG	2 cut(s)	1172, 2510
TatI	WGTACW	3 cut(s)	1859, 2667, 3197
TauI	GCSGC	3 cut(s)	1016, 1853, 2351
TscAI	CASTG	4 cut(s)	1529, 1675, 2867, 3013
TseFI	GTSAC	6 cut(s)	1102, 1648, 1756, 2440, 2986, 3094
TseI	GCWGC	6 cut(s)	477, 1714, 1976, 2096, 3188, 3319
Tsp45I	GTSAC	6 cut(s)	1102, 1648, 1756, 2440, 2986, 3094
TspGWI	ACGGA	3 cut(s)	1311, 2172, 2649
TspRI	CASTG	4 cut(s)	1529, 1675, 2867, 3013
VneI	GTGCAC	2 cut(s)	1635, 2973
VpaK11BI	GGWCC	2 cut(s)	1342, 2680
XapI	RAATTY	1 cut(s)	2239
XceI	RCATGY	2 cut(s)	146, 192
XcmI	CCANNNNNNNNNTGG	1 cut(s)	530
XmiI	GTMKAC	2 cut(s)	408, 2564
XmnI	GAANNNNTTC	3 cut(s)	851, 1739, 3077
XspI	CTAG	7 cut(s)	1292, 1434, 1607, 2042, 2630, 2772, 2945
ZraI	GACGTC	3 cut(s)	406, 1375, 2713
ZrmI	AGTACT	2 cut(s)	1861, 3199

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.