RLG00000010173

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0006735
Physical Location & Seq
Forward (+)
63852408 .. 63853141
734 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010173

Sequence Viewer

Length: 642 bp
ATGGAGGCACCAAAACTAGACTGCGGCTTCGCTAGGCTACCGACCGTCGCACCAGATCTCCGAGGCTTCGGCGACACCGACCTGTTGAGATACACTTACCTTCACGCTCTCTGCCACTCCAACTGCCTCGAAGATGCCCACCACCGCTTTGCCCACTCCACTGACTCAGATTGCGTCCCTGACCAGCGCACCTGCAACGTCATCATCGCTAGGCTACTTGATTCTCAAACCCCACACAGCACGTTGGGTGTTCGTCGCGTTTTGAGTGCACTGAAGCATGATTTTGTTGCCTCTTTGGTTAATTACAACCGTCTGATGGATGAGTTTTGCTCGCCCCGCGGCCCAGTGAGGCTGATAGAATGTTGTTTGATGAATGGTTATTATGAAATTGGCGAATTCGGTAATGCACAGAAGGTGTTTGACGAAACGTGTGAAAGGGGTGTAGCGCCTAATTCGATCGCTTATAGTGTGTTGGTTCGTGGCGTTCATCGGAAGTGGGATATTGGGGGTGCTATGGAAGTGATGGAAAAATTGTTTAGCAATTGGGATTCATACCGGAACTTGGTACATCAGATTGTACCAAGAAAAGGAGAAGCAGTTCAGTATGAACCGGTTGGTTCGACGTTGCCGGAGGTGGAGTAG

Protein Analysis

214

Amino Acids

24.0

Weight (kDa)

5.84

Isoelectric Point (pI)

27.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 122 - 162 2.7e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 200
Acc36I ACCTGC 1 cut(s) 200
AccB1I GGYRCC 1 cut(s) 7
AccII CGCG 2 cut(s) 258, 339
AciI CCGC 4 cut(s) 24, 145, 337, 339
AcsI RAATTY 1 cut(s) 395
AcuI CTGAAG 1 cut(s) 293
AfaI GTAC 2 cut(s) 567, 579
AfiI CCNNNNNNNGG 3 cut(s) 316, 562, 587
AflIII ACRYGT 1 cut(s) 428
AgeI ACCGGT 1 cut(s) 610
AloI GAACNNNNNNTCC 2 cut(s) 582, 614
Alw21I GWGCWC 1 cut(s) 271
Alw44I GTGCAC 1 cut(s) 267
AoxI GGCC 1 cut(s) 340
ApaLI GTGCAC 1 cut(s) 267
ApoI RAATTY 1 cut(s) 395
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AsiGI ACCGGT 1 cut(s) 610
Asp700I GAANNNNTTC 1 cut(s) 597
AspLEI GCGC 2 cut(s) 189, 448
AspS9I GGNCC 1 cut(s) 341
BaeGI GKGCMC 1 cut(s) 271
BanI GGYRCC 1 cut(s) 7
Bbv12I GWGCWC 1 cut(s) 271
BccI CCATC 2 cut(s) 310, 517
BfaI CTAG 3 cut(s) 17, 33, 210
BfoI RGCGCY 1 cut(s) 449
BfuAI ACCTGC 1 cut(s) 200
BglII AGATCT 1 cut(s) 55
BisI GCNGC 2 cut(s) 25, 340
BlsI GCNGC 2 cut(s) 26, 341
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 1 cut(s) 9
BmrI ACTGGG 1 cut(s) 338
BmsI GCATC 1 cut(s) 124
BmuI ACTGGG 1 cut(s) 338
BplI GAGNNNNNCTC 2 cut(s) 314, 346
BsaJI CCNNGG 2 cut(s) 61, 337
BsaWI WCCGGW 2 cut(s) 555, 610
BsaXI ACNNNNNCTCC 5 cut(s) 42, 72, 582, 612, 623
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 562, 587
Bse118I RCCGGY 1 cut(s) 610
Bse1I ACTGG 1 cut(s) 344
BseDI CCNNGG 2 cut(s) 61, 337
BseGI GGATG 1 cut(s) 325
BseLI CCNNNNNNNGG 3 cut(s) 316, 562, 587
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 271
Bsh1236I CGCG 2 cut(s) 258, 339
Bsh1285I CGRYCG 2 cut(s) 45, 459
BshFI GGCC 1 cut(s) 342
BshNI GGYRCC 1 cut(s) 7
BshTI ACCGGT 1 cut(s) 610
BsiEI CGRYCG 2 cut(s) 45, 459
BsiHKAI GWGCWC 1 cut(s) 271
BsiSI CCGG 3 cut(s) 556, 611, 629
BslFI GGGAC 1 cut(s) 161
BslI CCNNNNNNNGG 3 cut(s) 316, 562, 587
BsmFI GGGAC 1 cut(s) 161
BsnI GGCC 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 271
Bsp143I GATC 2 cut(s) 55, 456
BspACI CCGC 4 cut(s) 24, 145, 337, 339
BspANI GGCC 1 cut(s) 342
BspCNI CTCAG 1 cut(s) 179
BspFNI CGCG 2 cut(s) 258, 339
BspLI GGNNCC 1 cut(s) 9
BspMI ACCTGC 1 cut(s) 200
BspT107I GGYRCC 1 cut(s) 7
BsrFI RCCGGY 1 cut(s) 610
BsrI ACTGG 1 cut(s) 344
BssAI RCCGGY 1 cut(s) 610
BssECI CCNNGG 2 cut(s) 61, 337
BssMI GATC 2 cut(s) 55, 456
Bst4CI ACNGT 2 cut(s) 46, 311
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 1 cut(s) 166
BstDSI CCRYGG 1 cut(s) 337
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 2 cut(s) 258, 339
BstH2I RGCGCY 1 cut(s) 449
BstHHI GCGC 2 cut(s) 189, 448
BstKTI GATC 2 cut(s) 58, 459
BstMBI GATC 2 cut(s) 55, 456
BstMCI CGRYCG 2 cut(s) 45, 459
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 271
BstUI CGCG 2 cut(s) 258, 339
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BsuRI GGCC 1 cut(s) 342
BtgI CCRYGG 1 cut(s) 337
BtgZI GCGATG 1 cut(s) 190
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 3 cut(s) 159, 269, 351
BveI ACCTGC 1 cut(s) 200
Cac8I GCNNGC 1 cut(s) 332
CfoI GCGC 2 cut(s) 189, 448
Cfr10I RCCGGY 1 cut(s) 610
Cfr13I GGNCC 1 cut(s) 341
Cfr42I CCGCGG 1 cut(s) 340
CseI GACGC 1 cut(s) 163
Csp6I GTAC 2 cut(s) 566, 578
CspAI ACCGGT 1 cut(s) 610
CviAII CATG 1 cut(s) 278
CviJI RGCY 6 cut(s) 27, 37, 66, 214, 342, 352
CviKI_1 RGCY 6 cut(s) 27, 37, 66, 214, 342, 352
CviQI GTAC 2 cut(s) 566, 578
DdeI CTNAG 1 cut(s) 166
DpnI GATC 2 cut(s) 57, 458
DpnII GATC 2 cut(s) 55, 456
Eco57I CTGAAG 1 cut(s) 293
EcoRI GAATTC 1 cut(s) 395
FaeI CATG 1 cut(s) 281
FaiI YATR 6 cut(s) 279, 384, 465, 515, 553, 606
FaqI GGGAC 1 cut(s) 161
FatI CATG 1 cut(s) 277
FauI CCCGC 1 cut(s) 344
Fnu4HI GCNGC 2 cut(s) 25, 340
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 2 cut(s) 25, 340
FspBI CTAG 3 cut(s) 17, 33, 210
GlaI GCGC 2 cut(s) 188, 447
GluI GCNGC 2 cut(s) 25, 340
HaeII RGCGCY 1 cut(s) 449
HaeIII GGCC 1 cut(s) 342
HapII CCGG 3 cut(s) 556, 611, 629
HgaI GACGC 1 cut(s) 163
HhaI GCGC 2 cut(s) 189, 448
Hin1II CATG 1 cut(s) 281
Hin6I GCGC 2 cut(s) 187, 446
HinP1I GCGC 2 cut(s) 187, 446
HinfI GANTC 3 cut(s) 164, 221, 548
HpaII CCGG 3 cut(s) 556, 611, 629
Hpy166II GTNNAC 1 cut(s) 269
Hpy188I TCNGA 5 cut(s) 62, 169, 315, 492, 573
Hpy8I GTNNAC 1 cut(s) 269
Hpy99I CGWCG 3 cut(s) 50, 258, 625
HpyAV CCTTC 2 cut(s) 110, 406
HpyCH4III ACNGT 2 cut(s) 46, 311
HpyCH4IV ACGT 4 cut(s) 198, 242, 428, 623
HpyCH4V TGCA 3 cut(s) 195, 269, 407
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 1 cut(s) 166
HpySE526I ACGT 4 cut(s) 198, 242, 428, 623
Hsp92II CATG 1 cut(s) 281
HspAI GCGC 2 cut(s) 187, 446
KspI CCGCGG 1 cut(s) 340
Kzo9I GATC 2 cut(s) 55, 456
LpnPI CCDG 8 cut(s) 66, 95, 192, 197, 205, 357, 569, 624
LweI GCATC 1 cut(s) 124
MaeI CTAG 3 cut(s) 17, 33, 210
MaeII ACGT 4 cut(s) 198, 242, 428, 623
MalI GATC 2 cut(s) 57, 458
MboI GATC 2 cut(s) 55, 456
MboII GAAGA 1 cut(s) 143
MfeI CAATTG 1 cut(s) 541
MflI RGATCY 1 cut(s) 55
MhlI GDGCHC 1 cut(s) 271
MluCI AATT 6 cut(s) 301, 387, 395, 451, 530, 541
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 5 cut(s) 56, 137, 301, 342, 625
MroXI GAANNNNTTC 1 cut(s) 597
MseI TTAA 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 339
MspI CCGG 3 cut(s) 556, 611, 629
MunI CAATTG 1 cut(s) 541
MvnI CGCG 2 cut(s) 258, 339
MwoI GCNNNNNNNGC 1 cut(s) 336
NdeII GATC 2 cut(s) 55, 456
NlaIII CATG 1 cut(s) 281
NlaIV GGNNCC 1 cut(s) 9
PaqCI CACCTGC 1 cut(s) 200
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 597
PfeI GAWTC 2 cut(s) 221, 548
PinAI ACCGGT 1 cut(s) 610
PkrI GCNGC 2 cut(s) 26, 341
Ple19I CGATCG 1 cut(s) 459
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 1 cut(s) 341
PsuI RGATCY 1 cut(s) 55
PvuI CGATCG 1 cut(s) 459
RsaI GTAC 2 cut(s) 567, 579
RsaNI GTAC 2 cut(s) 566, 578
SacII CCGCGG 1 cut(s) 340
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 2 cut(s) 25, 340
Sau3AI GATC 2 cut(s) 55, 456
Sau96I GGNCC 1 cut(s) 341
SchI GAGTC 1 cut(s) 158
SduI GDGCHC 1 cut(s) 271
SetI ASST 9 cut(s) 84, 102, 194, 201, 245, 417, 431, 626, 636
SfaNI GCATC 1 cut(s) 124
Sfr303I CCGCGG 1 cut(s) 340
SgrBI CCGCGG 1 cut(s) 340
Sse9I AATT 6 cut(s) 301, 387, 395, 451, 530, 541
SsiI CCGC 4 cut(s) 24, 145, 337, 339
SspMI CTAG 3 cut(s) 17, 33, 210
TaaI ACNGT 2 cut(s) 46, 311
TaiI ACGT 4 cut(s) 201, 245, 431, 626
TaqI TCGA 3 cut(s) 129, 455, 620
TasI AATT 6 cut(s) 301, 387, 395, 451, 530, 541
TauI GCSGC 2 cut(s) 27, 342
TfiI GAWTC 2 cut(s) 221, 548
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 3 cut(s) 166, 276, 351
TspDTI ATGAA 5 cut(s) 386, 399, 476, 540, 621
TspRI CASTG 3 cut(s) 166, 276, 351
VneI GTGCAC 1 cut(s) 267
XapI RAATTY 1 cut(s) 395
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 3 cut(s) 17, 33, 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.