pycom08g02250

Bifunctional dihydroflavonol 4-reductase flavanone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002832
Physical Location & Seq
Forward (+)
1683303 .. 1683931
629 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 363 bp
ATGGGATCGAAGTCTGAATCCGTTTGTGTCACTGGCGCGTCCGGTTTCATCAGCTCCTGGCTCGTCATGAGACTCCTCGAGCGCGGTTACACCGTCCGCGCCACCGTGCGTGACCCCACGAATCGGAAGAAGGTGAAGCATATGTTGGACTTGCTGAAGGCGGAGACGCACCTGACGCTGTGGAAGGCGGATCTGGCGGACGAGGGAAGCTTTGATGAAGCCATACAAGGCTGCACCGGAGTGTTCCATGTTGCTACACCTATGGACTTTGAGCCCAAGGACCCCGAGAATGAAGTGATCAAGCCAACAATCAATGGACTTTTGGACATCCTGAAGGCATGCCAGAAAGTTAAAACAGTTTGA

Protein Analysis

121

Amino Acids

13.28

Weight (kDa)

6.82

Isoelectric Point (pI)

13.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 86 8.1e-11 NmrA-like family
Epimerase PF01370 8 - 117 1.6e-12 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 117 2.3e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 118 4.7e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 87 3e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 38, 84, 99
AciI CCGC 5 cut(s) 84, 97, 161, 188, 197
AclWI GGATC 2 cut(s) 13, 198
AcuI CTGAAG 2 cut(s) 176, 353
AfiI CCNNNNNNNGG 1 cut(s) 123
AjnI CCWGG 1 cut(s) 56
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AleI CACNNNNGTG 1 cut(s) 239
AluBI AGCT 2 cut(s) 54, 210
AluI AGCT 2 cut(s) 54, 210
Alw26I GTCTC 2 cut(s) 64, 158
AlwI GGATC 2 cut(s) 13, 198
AlwNI CAGNNNCTG 1 cut(s) 57
Ama87I CYCGRG 2 cut(s) 77, 284
ApeKI GCWGC 1 cut(s) 231
AspLEI GCGC 3 cut(s) 38, 84, 101
AspS9I GGNCC 1 cut(s) 280
AsuHPI GGTGA 1 cut(s) 145
AvaI CYCGRG 2 cut(s) 77, 284
AvaII GGWCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 218
BciT130I CCWGG 1 cut(s) 58
BclI TGATCA 1 cut(s) 297
BcoDI GTCTC 2 cut(s) 64, 158
BisI GCNGC 1 cut(s) 232
BlsI GCNGC 1 cut(s) 233
Bme1390I CCNGG 1 cut(s) 58
Bme18I GGWCC 1 cut(s) 280
BmeT110I CYCGRG 2 cut(s) 77, 284
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 1 cut(s) 282
BmrFI CCNGG 1 cut(s) 58
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 2 cut(s) 41, 236
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 37
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 1 cut(s) 327
BseLI CCNNNNNNNGG 1 cut(s) 123
BseNI ACTGG 1 cut(s) 37
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 1 cut(s) 218
BsgI GTGCAG 1 cut(s) 217
Bsh1236I CGCG 3 cut(s) 38, 84, 99
BsiHKCI CYCGRG 2 cut(s) 77, 284
BsiSI CCGG 2 cut(s) 42, 237
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 2 cut(s) 64, 158
BsmBI CGTCTC 1 cut(s) 158
BsoBI CYCGRG 2 cut(s) 77, 284
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 3 cut(s) 5, 190, 297
BspACI CCGC 5 cut(s) 84, 97, 161, 188, 197
BspFNI CGCG 3 cut(s) 38, 84, 99
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 282
BspPI GGATC 2 cut(s) 13, 198
BsrI ACTGG 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 3 cut(s) 5, 190, 297
BssT1I CCWWGG 1 cut(s) 276
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 3 cut(s) 94, 106, 358
BstC8I GCNNGC 1 cut(s) 340
BstF5I GGATG 1 cut(s) 327
BstFNI CGCG 3 cut(s) 38, 84, 99
BstHHI GCGC 3 cut(s) 38, 84, 101
BstKTI GATC 3 cut(s) 8, 193, 300
BstMAI GTCTC 2 cut(s) 64, 158
BstMBI GATC 3 cut(s) 5, 190, 297
BstMWI GCNNNNNNNGC 2 cut(s) 175, 194
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 56
BstUI CGCG 3 cut(s) 38, 84, 99
BstV1I GCAGC 1 cut(s) 218
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BtsCI GGATG 1 cut(s) 327
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 1 cut(s) 340
CaiI CAGNNNCTG 1 cut(s) 57
CciI TCATGA 1 cut(s) 66
CfoI GCGC 3 cut(s) 38, 84, 101
Cfr13I GGNCC 1 cut(s) 280
CseI GACGC 3 cut(s) 27, 175, 184
CviAII CATG 3 cut(s) 67, 248, 339
CviJI RGCY 7 cut(s) 54, 61, 210, 221, 231, 274, 304
CviKI_1 RGCY 7 cut(s) 54, 61, 210, 221, 231, 274, 304
DpnI GATC 3 cut(s) 7, 192, 299
DpnII GATC 3 cut(s) 5, 190, 297
EciI GGCGGA 3 cut(s) 176, 203, 212
Eco130I CCWWGG 1 cut(s) 276
Eco24I GRGCYC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 2 cut(s) 176, 353
Eco88I CYCGRG 2 cut(s) 77, 284
EcoO109I RGGNCCY 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 276
Esp3I CGTCTC 1 cut(s) 158
FaeI CATG 3 cut(s) 70, 251, 342
FaiI YATR 7 cut(s) 68, 141, 143, 224, 249, 263, 340
FatI CATG 3 cut(s) 66, 247, 338
FauNDI CATATG 1 cut(s) 141
FbaI TGATCA 1 cut(s) 297
Fnu4HI GCNGC 1 cut(s) 232
FokI GGATG 1 cut(s) 314
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 1 cut(s) 232
GlaI GCGC 3 cut(s) 37, 83, 100
GluI GCNGC 1 cut(s) 232
HapII CCGG 2 cut(s) 42, 237
HgaI GACGC 3 cut(s) 27, 175, 184
HhaI GCGC 3 cut(s) 38, 84, 101
Hin1II CATG 3 cut(s) 70, 251, 342
Hin6I GCGC 3 cut(s) 36, 82, 99
HinP1I GCGC 3 cut(s) 36, 82, 99
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 3 cut(s) 17, 72, 121
HpaII CCGG 2 cut(s) 42, 237
HphI GGTGA 1 cut(s) 145
Hpy188I TCNGA 2 cut(s) 16, 126
Hpy188III TCNNGA 2 cut(s) 67, 331
HpyAV CCTTC 4 cut(s) 124, 151, 178, 328
HpyCH4III ACNGT 3 cut(s) 94, 106, 358
HpyCH4V TGCA 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 194
Hsp92II CATG 3 cut(s) 70, 251, 342
HspAI GCGC 3 cut(s) 36, 82, 99
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 3 cut(s) 5, 190, 297
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 9 cut(s) 18, 43, 55, 70, 179, 185, 250, 344, 356
Lsp1109I GCAGC 1 cut(s) 218
MaeIII GTNAC 3 cut(s) 28, 86, 110
MalI GATC 3 cut(s) 7, 192, 299
MboI GATC 3 cut(s) 5, 190, 297
MboII GAAGA 1 cut(s) 139
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 276
MlyI GAGTC 1 cut(s) 66
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 2 cut(s) 86, 196
MseI TTAA 1 cut(s) 351
MslI CAYNNNNRTG 1 cut(s) 239
MspI CCGG 2 cut(s) 42, 237
MspR9I CCNGG 1 cut(s) 58
MvaI CCWGG 1 cut(s) 58
MvnI CGCG 3 cut(s) 38, 84, 99
MwoI GCNNNNNNNGC 2 cut(s) 175, 194
NdeI CATATG 1 cut(s) 141
NdeII GATC 3 cut(s) 5, 190, 297
NlaIII CATG 3 cut(s) 70, 251, 342
NlaIV GGNNCC 1 cut(s) 282
NmuCI GTSAC 2 cut(s) 28, 110
NspI RCATGY 1 cut(s) 342
OliI CACNNNNGTG 1 cut(s) 239
PaeI GCATGC 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 77
PagI TCATGA 1 cut(s) 66
PfeI GAWTC 2 cut(s) 17, 121
PkrI GCNGC 1 cut(s) 233
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 1 cut(s) 282
PspPI GGNCC 1 cut(s) 280
PspPPI RGGWCCY 1 cut(s) 280
PspXI VCTCGAGB 1 cut(s) 77
PstNI CAGNNNCTG 1 cut(s) 57
PsuI RGATCY 1 cut(s) 190
RseI CAYNNNNRTG 1 cut(s) 239
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 1 cut(s) 232
Sau3AI GATC 3 cut(s) 5, 190, 297
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 58
SduI GDGCHC 1 cut(s) 276
SetI ASST 5 cut(s) 56, 135, 174, 212, 262
Sfr274I CTCGAG 1 cut(s) 77
SinI GGWCC 1 cut(s) 280
SlaI CTCGAG 1 cut(s) 77
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SphI GCATGC 1 cut(s) 342
SsiI CCGC 5 cut(s) 84, 97, 161, 188, 197
StyD4I CCNGG 1 cut(s) 56
StyI CCWWGG 1 cut(s) 276
TaaI ACNGT 3 cut(s) 94, 106, 358
TaqI TCGA 2 cut(s) 8, 78
TfiI GAWTC 2 cut(s) 17, 121
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 37
TseFI GTSAC 2 cut(s) 28, 110
TseI GCWGC 1 cut(s) 231
Tsp45I GTSAC 2 cut(s) 28, 110
TspDTI ATGAA 3 cut(s) 37, 231, 306
TspGWI ACGGA 1 cut(s) 10
TspRI CASTG 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 280
XceI RCATGY 1 cut(s) 342
XhoI CTCGAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.