pycom08g05150

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008302
Physical Location & Seq
Forward (+)
3959847 .. 3960227
381 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05150.1

Sequence Viewer

Length: 381 bp
ATGAACAGCTCGTTCACTCCCACTGTTCTCTTCCTCCTCCTCAATGTCATGATTGGCACCATCGCCATTGCTTCAAACTTGGGTTCCACCCAGAAACACCAGGACCCAGAGCAGCACCACCACCAGAACCATCACCAAGGCGGATGCCTCGCCAGATCTCCGTCTATGCTGCAGAGGCTCGAATCCATCAACCTCTACCAGTACAGATCCCCGGAGCCACACGCCGCCACTTTCGAGAAACACCCGGAAACTACTGAGGATACCCATTACGCCGTTAACCACGCCCACGAACTTGAACATGATTGTGAGAGGAGAGACCAAATCCATATATATCCAACTGTTGACAGTTTCCTCCTATTAAGGAAAACTGTCATCCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

127

Amino Acids

14.47

Weight (kDa)

6.19

Isoelectric Point (pI)

57.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 1 - 25 2.7e-08 Domain of unknown function (DUF4408)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 56
AciI CCGC 2 cut(s) 141, 225
AclWI GGATC 1 cut(s) 201
AfaI GTAC 1 cut(s) 203
AgsI TTSAA 2 cut(s) 75, 296
AjnI CCWGG 1 cut(s) 99
AluBI AGCT 1 cut(s) 9
AluI AGCT 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 309
AlwI GGATC 1 cut(s) 201
ApeKI GCWGC 2 cut(s) 112, 169
AspS9I GGNCC 1 cut(s) 103
AsuC2I CCSGG 2 cut(s) 212, 245
AsuHPI GGTGA 1 cut(s) 125
AvaII GGWCC 1 cut(s) 103
BanI GGYRCC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 124, 156
BccI CCATC 3 cut(s) 68, 138, 194
BceAI ACGGC 1 cut(s) 257
BciT130I CCWGG 1 cut(s) 101
BciVI GTATCC 1 cut(s) 253
BcnI CCSGG 2 cut(s) 212, 245
BcoDI GTCTC 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 170
BfuI GTATCC 1 cut(s) 253
BglII AGATCT 1 cut(s) 155
BisI GCNGC 3 cut(s) 113, 170, 225
BlsI GCNGC 3 cut(s) 114, 171, 226
Bme1390I CCNGG 3 cut(s) 101, 212, 245
Bme18I GGWCC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 4 cut(s) 58, 85, 105, 216
BmrFI CCNGG 3 cut(s) 101, 212, 245
BmsI GCATC 1 cut(s) 134
BpuMI CCSGG 2 cut(s) 212, 245
BsaI GGTCTC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 136, 210
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 1 cut(s) 66
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 2 cut(s) 136, 210
BseGI GGATG 2 cut(s) 149, 372
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 3 cut(s) 26, 29, 325
BseXI GCAGC 2 cut(s) 124, 156
BshNI GGYRCC 1 cut(s) 56
BsiSI CCGG 2 cut(s) 212, 245
BsmAI GTCTC 1 cut(s) 309
Bso31I GGTCTC 1 cut(s) 309
Bsp143I GATC 2 cut(s) 155, 206
BspACI CCGC 2 cut(s) 141, 225
BspCNI CTCAG 1 cut(s) 247
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 4 cut(s) 58, 85, 105, 216
BspMAI CTGCAG 1 cut(s) 174
BspPI GGATC 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 309
BsrDI GCAATG 1 cut(s) 66
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 2 cut(s) 136, 210
BssMI GATC 2 cut(s) 155, 206
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 101
Bst4CI ACNGT 4 cut(s) 25, 340, 347, 370
Bst6I CTCTTC 1 cut(s) 35
BstDEI CTNAG 1 cut(s) 255
BstF5I GGATG 2 cut(s) 149, 372
BstKTI GATC 2 cut(s) 158, 209
BstMAI GTCTC 1 cut(s) 309
BstMBI GATC 2 cut(s) 155, 206
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 1 cut(s) 101
BstSCI CCNGG 3 cut(s) 99, 210, 243
BstSFI CTRYAG 1 cut(s) 170
BstV1I GCAGC 2 cut(s) 124, 156
BstX2I RGATCY 2 cut(s) 155, 206
BstYI RGATCY 2 cut(s) 155, 206
BsuI GTATCC 1 cut(s) 253
BtgZI GCGATG 1 cut(s) 46
BtsCI GGATG 2 cut(s) 149, 372
BtsIMutI CAGTG 1 cut(s) 21
CciI TCATGA 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 103
Csp6I GTAC 1 cut(s) 202
CviAII CATG 2 cut(s) 49, 299
CviJI RGCY 3 cut(s) 9, 178, 217
CviKI_1 RGCY 3 cut(s) 9, 178, 217
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 1 cut(s) 255
DpnI GATC 2 cut(s) 157, 208
DpnII GATC 2 cut(s) 155, 206
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
EciI GGCGGA 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 99
EcoT14I CCWWGG 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 2 cut(s) 52, 302
FaiI YATR 7 cut(s) 50, 167, 300, 327, 329, 331, 379
FatI CATG 2 cut(s) 48, 298
Fnu4HI GCNGC 3 cut(s) 113, 170, 225
FokI GGATG 2 cut(s) 156, 359
Fsp4HI GCNGC 3 cut(s) 113, 170, 225
GluI GCNGC 3 cut(s) 113, 170, 225
HapII CCGG 2 cut(s) 212, 245
Hin1II CATG 2 cut(s) 52, 302
HincII GTYRAC 2 cut(s) 277, 343
HindII GTYRAC 2 cut(s) 277, 343
HinfI GANTC 1 cut(s) 182
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 2 cut(s) 212, 245
HphI GGTGA 1 cut(s) 125
Hpy166II GTNNAC 3 cut(s) 15, 277, 343
Hpy188III TCNNGA 2 cut(s) 49, 235
Hpy8I GTNNAC 3 cut(s) 15, 277, 343
HpyCH4III ACNGT 4 cut(s) 25, 340, 347, 370
HpyCH4V TGCA 1 cut(s) 172
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 1 cut(s) 255
Hsp92II CATG 2 cut(s) 52, 302
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 2 cut(s) 155, 206
LmnI GCTCC 1 cut(s) 214
LpnPI CCDG 9 cut(s) 86, 104, 113, 120, 137, 166, 212, 225, 258
Lsp1109I GCAGC 2 cut(s) 124, 156
LweI GCATC 1 cut(s) 134
MalI GATC 2 cut(s) 157, 208
MboI GATC 2 cut(s) 155, 206
MboII GAAGA 1 cut(s) 22
MflI RGATCY 2 cut(s) 155, 206
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 9 cut(s) 44, 47, 50, 158, 168, 203, 250, 303, 362
MseI TTAA 2 cut(s) 276, 359
MslI CAYNNNNRTG 1 cut(s) 303
MspI CCGG 2 cut(s) 212, 245
MspR9I CCNGG 3 cut(s) 101, 212, 245
MvaI CCWGG 1 cut(s) 101
MwoI GCNNNNNNNGC 1 cut(s) 175
NciI CCSGG 2 cut(s) 212, 245
NdeII GATC 2 cut(s) 155, 206
NlaIII CATG 2 cut(s) 52, 302
NlaIV GGNNCC 4 cut(s) 58, 85, 105, 216
PagI TCATGA 1 cut(s) 48
PfeI GAWTC 1 cut(s) 182
PkrI GCNGC 3 cut(s) 114, 171, 226
PpuMI RGGWCCY 1 cut(s) 103
Psp5II RGGWCCY 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 4 cut(s) 58, 85, 105, 216
PspPI GGNCC 1 cut(s) 103
PspPPI RGGWCCY 1 cut(s) 103
PstI CTGCAG 1 cut(s) 174
PsuI RGATCY 2 cut(s) 155, 206
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 2 cut(s) 276, 359
SatI GCNGC 3 cut(s) 113, 170, 225
Sau3AI GATC 2 cut(s) 155, 206
Sau96I GGNCC 1 cut(s) 103
ScrFI CCNGG 3 cut(s) 101, 212, 245
SetI ASST 2 cut(s) 11, 195
SfaNI GCATC 1 cut(s) 134
SfcI CTRYAG 1 cut(s) 170
SinI GGWCC 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 303
SsiI CCGC 2 cut(s) 141, 225
StyD4I CCNGG 3 cut(s) 99, 210, 243
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 4 cut(s) 25, 340, 347, 370
TaqI TCGA 2 cut(s) 180, 234
TatI WGTACW 1 cut(s) 201
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 182
Tru1I TTAA 2 cut(s) 276, 359
Tru9I TTAA 2 cut(s) 276, 359
TscAI CASTG 1 cut(s) 28
TseI GCWGC 2 cut(s) 112, 169
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.