pycom08g09180

Uncharacterized protein family UPF0054

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003247
Physical Location & Seq
Reverse (-)
7423380 .. 7430747
7368 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1158 bp
ATGCCCCTGATCTTAAGCTTCCGATGGTTGGGTGACATTGTGATTTCTGTTGAGACAGCGGCAAGGCAAGCAGAGGAAAGAGGCCACACGCTTATTGATGAGATACGTGTTCTCATGATACATGGGTTGTTACATTTACTAGGATTTGATCATGAGATTAGTGAAGAGGCCGAAGTGGAAATGGAGAAGGAGGAGGAGCTTCTTTTAAAGAGTCTTGGGTGGAAGGGGAAAGGATTAATTCAAAGTGCACATGATGCCGAAACCCAAACAAATTCCCATGAGGAAAAACCAGATGACAGGAAGAGAGAAGGCAGCCTGCGATTTTATAAACCAAAGTTCAAATGCATTTTCTGTGATATGGATGGCACCCTTCTCAACAGCAAAAGTCAAATTAGTTTAACAACTGCGAAAGCTCTCAAGGAGGTCTCATCAAGGGGTGTGAGAGTAGTGATAGCCACGGGAAAATCTCGTTCAGCTGTGATGAAAATTTTTAAGGAGGTAGAATTAGCTGGAGAAGATGGCATTGTTTCGGAATTTTCTCCTGGGGTTTTTTTGCAGGGATTGCTTGTTTATGGTAGGCAAGGTCGGGAAATTTTTAGAAAAAACTTAGATACAAGTGTCTGCAGAGAGGCGTGTCTCTACTCTTGGGAGAATAAGGTTCCTCTTATTGCATTCAGCAGGGACCGTTGCTTTTCTCTGTTTGAGCATCCATTACTTGACTCAGTTCATACATTGTATAATGAGCCAAAGGTGGAAATCATGCCTTCAGTTGAGCACCTCTTGGCTGAAGCTGACATACAGAAAGTGCTCTTCATGGAGACCCCTGAGGTAGTGGCTACTACTTTGAGGCCATACTGGTCACAAGCAACTGGAGATCGTGCAACAGCTGTTCAAGCAGTGGAAGGGCACCTTGAAATTGTTCCTGCAGGAACCTCAAAAGGAAGTGGGGTAAACATGCTTCTTGATCATTTAGGAATCACTCCAAATCAGATCATGGCTATTGGTGATGGAGAAAATGATGTCGAGATGCTTGAGCTGGCTTCTTTGGGCGTTGCTCTTAGTAATGGATCAGAGAAGACAAAAGCTGTGGCTAATGTAATTGGTGCAAGCAATGATGAAGATGGTGCGGCTGATGCCATTTACCGGTACGCATTTTGA

Protein Analysis

386

Amino Acids

42.56

Weight (kDa)

5.13

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YbeY PF02130 10 - 71 5.4e-20 Endoribonuclease YbeY
Hydrolase_3 PF08282 116 - 380 6.5e-52 haloacid dehalogenase-like hydrolase
Hydrolase PF00702 289 - 347 1.4e-06 haloacid dehalogenase-like hydrolase
S6PP PF05116 294 - 359 1.2e-09 Sucrose-6F-phosphate phosphohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
AccB1I GGYRCC 2 cut(s) 365, 906
AciI CCGC 2 cut(s) 59, 1127
AclWI GGATC 1 cut(s) 1075
AcsI RAATTY 4 cut(s) 271, 486, 533, 591
AcuI CTGAAG 2 cut(s) 750, 807
AfaI GTAC 1 cut(s) 1148
AfiI CCNNNNNNNGG 2 cut(s) 28, 1143
AflII CTTAAG 1 cut(s) 13
AflIII ACRYGT 1 cut(s) 106
AgeI ACCGGT 1 cut(s) 1143
AgsI TTSAA 4 cut(s) 242, 340, 893, 914
AjnI CCWGG 1 cut(s) 541
AluBI AGCT 9 cut(s) 18, 199, 413, 476, 509, 791, 887, 1036, 1085
AluI AGCT 9 cut(s) 18, 199, 413, 476, 509, 791, 887, 1036, 1085
Alw21I GWGCWC 3 cut(s) 250, 777, 810
Alw26I GTCTC 4 cut(s) 47, 430, 641, 812
Alw44I GTGCAC 1 cut(s) 246
AlwI GGATC 1 cut(s) 1075
AoxI GGCC 3 cut(s) 82, 168, 848
ApaLI GTGCAC 1 cut(s) 246
ApeKI GCWGC 1 cut(s) 312
ApoI RAATTY 4 cut(s) 271, 486, 533, 591
AseI ATTAAT 1 cut(s) 236
AsiGI ACCGGT 1 cut(s) 1143
Asp700I GAANNNNTTC 1 cut(s) 918
AspS9I GGNCC 1 cut(s) 682
AsuHPI GGTGA 2 cut(s) 44, 1016
AvaII GGWCC 1 cut(s) 682
AxyI CCTNAGG 1 cut(s) 825
BaeGI GKGCMC 2 cut(s) 250, 909
BaeI ACNNNNGTAYC 4 cut(s) 110, 143, 603, 636
BanI GGYRCC 2 cut(s) 365, 906
BarI GAAGNNNNNNTAC 4 cut(s) 780, 812, 942, 974
BbsI GAAGAC 1 cut(s) 1082
Bbv12I GWGCWC 3 cut(s) 250, 777, 810
BbvI GCAGC 1 cut(s) 324
BccI CCATC 5 cut(s) 18, 356, 512, 1001, 1115
BciT130I CCWGG 1 cut(s) 543
BclI TGATCA 2 cut(s) 148, 964
BcoDI GTCTC 4 cut(s) 47, 430, 641, 812
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 2 cut(s) 622, 924
BfrI CTTAAG 1 cut(s) 13
BisI GCNGC 3 cut(s) 60, 313, 1128
BlsI GCNGC 3 cut(s) 61, 314, 1129
Bme1390I CCNGG 1 cut(s) 543
Bme18I GGWCC 1 cut(s) 682
BmgT120I GGNCC 1 cut(s) 682
BmiI GGNNCC 5 cut(s) 367, 660, 683, 908, 931
BmrFI CCNGG 1 cut(s) 543
BmsI GCATC 4 cut(s) 244, 715, 1017, 1123
BpiI GAAGAC 1 cut(s) 1082
BpmI CTGGAG 2 cut(s) 531, 891
BpuEI CTTGAG 2 cut(s) 401, 1052
BsaAI YACGTR 1 cut(s) 107
BsaI GGTCTC 2 cut(s) 430, 812
BsaJI CCNNGG 2 cut(s) 456, 542
BsaWI WCCGGW 1 cut(s) 1143
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 1143
Bse118I RCCGGY 1 cut(s) 1143
Bse1I ACTGG 2 cut(s) 860, 874
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 1 cut(s) 1117
BseBI CCWGG 1 cut(s) 543
BseDI CCNNGG 2 cut(s) 456, 542
BseGI GGATG 2 cut(s) 367, 706
BseLI CCNNNNNNNGG 2 cut(s) 28, 1143
BseMI GCAATG 1 cut(s) 1117
BseMII CTCAG 2 cut(s) 735, 816
BseNI ACTGG 2 cut(s) 860, 874
BseRI GAGGAG 2 cut(s) 206, 209
BseSI GKGCMC 2 cut(s) 250, 909
BseXI GCAGC 1 cut(s) 324
BshFI GGCC 3 cut(s) 84, 170, 850
BshNI GGYRCC 2 cut(s) 365, 906
BshTI ACCGGT 1 cut(s) 1143
BsiHKAI GWGCWC 3 cut(s) 250, 777, 810
BsiSI CCGG 1 cut(s) 1144
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 2 cut(s) 28, 1143
BsmAI GTCTC 4 cut(s) 47, 430, 641, 812
BsmFI GGGAC 1 cut(s) 695
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 3 cut(s) 84, 170, 850
Bso31I GGTCTC 2 cut(s) 430, 812
Bsp1286I GDGCHC 4 cut(s) 250, 777, 810, 909
Bsp143I GATC 6 cut(s) 9, 148, 874, 964, 990, 1067
BspACI CCGC 2 cut(s) 59, 1127
BspANI GGCC 3 cut(s) 84, 170, 850
BspCNI CTCAG 2 cut(s) 734, 817
BspHI TCATGA 2 cut(s) 114, 151
BspLI GGNNCC 5 cut(s) 367, 660, 683, 908, 931
BspMAI CTGCAG 2 cut(s) 626, 928
BspPI GGATC 1 cut(s) 1075
BspQI GCTCTTC 1 cut(s) 815
BspT107I GGYRCC 2 cut(s) 365, 906
BspTI CTTAAG 1 cut(s) 13
BspTNI GGTCTC 2 cut(s) 430, 812
BsrDI GCAATG 1 cut(s) 1117
BsrFI RCCGGY 1 cut(s) 1143
BsrI ACTGG 2 cut(s) 860, 874
BssAI RCCGGY 1 cut(s) 1143
BssECI CCNNGG 2 cut(s) 456, 542
BssMI GATC 6 cut(s) 9, 148, 874, 964, 990, 1067
Bst2UI CCWGG 1 cut(s) 543
Bst4CI ACNGT 1 cut(s) 686
Bst6I CTCTTC 3 cut(s) 159, 296, 815
BstAFI CTTAAG 1 cut(s) 13
BstAPI GCANNNNNTGC 2 cut(s) 254, 562
BstBAI YACGTR 1 cut(s) 107
BstC8I GCNNGC 4 cut(s) 69, 317, 1038, 1108
BstDEI CTNAG 4 cut(s) 607, 721, 825, 1058
BstDSI CCRYGG 1 cut(s) 456
BstF5I GGATG 2 cut(s) 367, 706
BstKTI GATC 6 cut(s) 12, 151, 877, 967, 993, 1070
BstMAI GTCTC 4 cut(s) 47, 430, 641, 812
BstMBI GATC 6 cut(s) 9, 148, 874, 964, 990, 1067
BstMWI GCNNNNNNNGC 5 cut(s) 68, 254, 562, 893, 1133
BstNI CCWGG 1 cut(s) 543
BstNSI RCATGY 1 cut(s) 958
BstSCI CCNGG 1 cut(s) 541
BstSFI CTRYAG 2 cut(s) 622, 924
BstSLI GKGCMC 2 cut(s) 250, 909
BstV1I GCAGC 1 cut(s) 324
BstV2I GAAGAC 1 cut(s) 1082
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 3 cut(s) 84, 170, 850
BtgI CCRYGG 1 cut(s) 456
BtsCI GGATG 2 cut(s) 367, 706
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 1 cut(s) 903
Cac8I GCNNGC 4 cut(s) 69, 317, 1038, 1108
CciI TCATGA 2 cut(s) 114, 151
Cfr10I RCCGGY 1 cut(s) 1143
Cfr13I GGNCC 1 cut(s) 682
Csp6I GTAC 1 cut(s) 1147
CspAI ACCGGT 1 cut(s) 1143
CspCI CAANNNNNGTGG 2 cut(s) 1068, 1103
CviAII CATG 9 cut(s) 115, 122, 152, 251, 278, 760, 814, 955, 994
CviQI GTAC 1 cut(s) 1147
DdeI CTNAG 4 cut(s) 607, 721, 825, 1058
DpnI GATC 6 cut(s) 11, 150, 876, 966, 992, 1069
DpnII GATC 6 cut(s) 9, 148, 874, 964, 990, 1067
DraI TTTAAA 1 cut(s) 207
Eam1104I CTCTTC 3 cut(s) 159, 296, 815
EarI CTCTTC 3 cut(s) 159, 296, 815
Eco31I GGTCTC 2 cut(s) 430, 812
Eco47I GGWCC 1 cut(s) 682
Eco57I CTGAAG 2 cut(s) 750, 807
Eco81I CCTNAGG 1 cut(s) 825
EcoRII CCWGG 1 cut(s) 541
EcoT22I ATGCAT 1 cut(s) 347
FaeI CATG 9 cut(s) 118, 125, 155, 254, 281, 763, 817, 958, 997
FalI AAGNNNNNCTT 2 cut(s) 894, 926
FaqI GGGAC 1 cut(s) 695
FatI CATG 9 cut(s) 114, 121, 151, 250, 277, 759, 813, 954, 993
FbaI TGATCA 2 cut(s) 148, 964
Fnu4HI GCNGC 3 cut(s) 60, 313, 1128
FokI GGATG 2 cut(s) 374, 693
Fsp4HI GCNGC 3 cut(s) 60, 313, 1128
FspBI CTAG 1 cut(s) 140
GluI GCNGC 3 cut(s) 60, 313, 1128
GsuI CTGGAG 2 cut(s) 531, 891
HaeIII GGCC 3 cut(s) 84, 170, 850
HapII CCGG 1 cut(s) 1144
Hin1II CATG 9 cut(s) 118, 125, 155, 254, 281, 763, 817, 958, 997
HindIII AAGCTT 1 cut(s) 16
HinfI GANTC 3 cut(s) 211, 719, 975
HpaII CCGG 1 cut(s) 1144
HphI GGTGA 2 cut(s) 44, 1016
Hpy166II GTNNAC 2 cut(s) 248, 952
Hpy188I TCNGA 4 cut(s) 23, 532, 990, 1072
Hpy188III TCNNGA 5 cut(s) 115, 152, 587, 962, 1024
Hpy8I GTNNAC 2 cut(s) 248, 952
HpyAV CCTTC 6 cut(s) 181, 217, 302, 380, 774, 896
HpyCH4III ACNGT 1 cut(s) 686
HpyCH4IV ACGT 1 cut(s) 106
HpyCH4V TGCA 8 cut(s) 248, 345, 556, 624, 671, 881, 926, 1106
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 254, 562, 893, 1133
HpyF3I CTNAG 4 cut(s) 607, 721, 825, 1058
HpySE526I ACGT 1 cut(s) 106
Hsp92II CATG 9 cut(s) 118, 125, 155, 254, 281, 763, 817, 958, 997
Ksp22I TGATCA 2 cut(s) 148, 964
Kzo9I GATC 6 cut(s) 9, 148, 874, 964, 990, 1067
LguI GCTCTTC 1 cut(s) 815
LmnI GCTCC 1 cut(s) 196
Lsp1109I GCAGC 1 cut(s) 324
LweI GCATC 4 cut(s) 244, 715, 1017, 1123
MaeI CTAG 1 cut(s) 140
MaeII ACGT 1 cut(s) 106
MaeIII GTNAC 3 cut(s) 32, 129, 858
MalI GATC 6 cut(s) 11, 150, 876, 966, 992, 1069
MboI GATC 6 cut(s) 9, 148, 874, 964, 990, 1067
MboII GAAGA 6 cut(s) 176, 313, 527, 802, 1087, 1130
MhlI GDGCHC 4 cut(s) 250, 777, 810, 909
MluCI AATT 9 cut(s) 237, 271, 390, 486, 503, 533, 591, 915, 1098
MlyI GAGTC 2 cut(s) 220, 713
Mph1103I ATGCAT 1 cut(s) 347
MroXI GAANNNNTTC 1 cut(s) 918
MseI TTAA 5 cut(s) 14, 206, 236, 398, 492
MspA1I CMGCKG 3 cut(s) 59, 476, 887
MspCI CTTAAG 1 cut(s) 13
MspI CCGG 1 cut(s) 1144
MspR9I CCNGG 1 cut(s) 543
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 1 cut(s) 543
MwoI GCNNNNNNNGC 5 cut(s) 68, 254, 562, 893, 1133
NdeII GATC 6 cut(s) 9, 148, 874, 964, 990, 1067
NlaIII CATG 9 cut(s) 118, 125, 155, 254, 281, 763, 817, 958, 997
NlaIV GGNNCC 5 cut(s) 367, 660, 683, 908, 931
NmuCI GTSAC 2 cut(s) 32, 858
NsiI ATGCAT 1 cut(s) 347
NspI RCATGY 1 cut(s) 958
PagI TCATGA 2 cut(s) 114, 151
PciSI GCTCTTC 1 cut(s) 815
PctI GAATGC 1 cut(s) 671
PdmI GAANNNNTTC 1 cut(s) 918
PfeI GAWTC 1 cut(s) 975
PinAI ACCGGT 1 cut(s) 1143
PkrI GCNGC 3 cut(s) 61, 314, 1129
PleI GAGTC 2 cut(s) 219, 713
PpsI GAGTC 2 cut(s) 219, 713
Ppu21I YACGTR 1 cut(s) 107
PshBI ATTAAT 1 cut(s) 236
PsiI TTATAA 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 541
PspGI CCWGG 1 cut(s) 541
PspN4I GGNNCC 5 cut(s) 367, 660, 683, 908, 931
PspPI GGNCC 1 cut(s) 682
PstI CTGCAG 2 cut(s) 626, 928
PvuII CAGCTG 2 cut(s) 476, 887
RsaI GTAC 1 cut(s) 1148
RsaNI GTAC 1 cut(s) 1147
SapI GCTCTTC 1 cut(s) 815
SaqAI TTAA 5 cut(s) 14, 206, 236, 398, 492
SatI GCNGC 3 cut(s) 60, 313, 1128
Sau3AI GATC 6 cut(s) 9, 148, 874, 964, 990, 1067
Sau96I GGNCC 1 cut(s) 682
SbfI CCTGCAGG 1 cut(s) 928
SchI GAGTC 2 cut(s) 220, 713
ScrFI CCNGG 1 cut(s) 543
SdaI CCTGCAGG 1 cut(s) 928
SduI GDGCHC 4 cut(s) 250, 777, 810, 909
SfaNI GCATC 4 cut(s) 244, 715, 1017, 1123
SfcI CTRYAG 2 cut(s) 622, 924
SinI GGWCC 1 cut(s) 682
SmlI CTYRAG 3 cut(s) 13, 416, 1031
SmoI CTYRAG 3 cut(s) 13, 416, 1031
Sse8387I CCTGCAGG 1 cut(s) 928
Sse9I AATT 9 cut(s) 237, 271, 390, 486, 503, 533, 591, 915, 1098
SsiI CCGC 2 cut(s) 59, 1127
SspMI CTAG 1 cut(s) 140
StyD4I CCNGG 1 cut(s) 541
TaaI ACNGT 1 cut(s) 686
TaiI ACGT 1 cut(s) 109
TaqI TCGA 1 cut(s) 1023
TasI AATT 9 cut(s) 237, 271, 390, 486, 503, 533, 591, 915, 1098
TauI GCSGC 2 cut(s) 62, 1130
TfiI GAWTC 1 cut(s) 975
Tru1I TTAA 5 cut(s) 14, 206, 236, 398, 492
Tru9I TTAA 5 cut(s) 14, 206, 236, 398, 492
TscAI CASTG 1 cut(s) 903
TseFI GTSAC 2 cut(s) 32, 858
TseI GCWGC 1 cut(s) 312
Tsp45I GTSAC 2 cut(s) 32, 858
TspDTI ATGAA 4 cut(s) 497, 716, 802, 1131
TspRI CASTG 1 cut(s) 903
Vha464I CTTAAG 1 cut(s) 13
VneI GTGCAC 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 682
VspI ATTAAT 1 cut(s) 236
XapI RAATTY 4 cut(s) 271, 486, 533, 591
XceI RCATGY 1 cut(s) 958
XmnI GAANNNNTTC 1 cut(s) 918
XspI CTAG 1 cut(s) 140
Zsp2I ATGCAT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.