pycom08g12740

Aux IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003240
Physical Location & Seq
Reverse (-)
11297306 .. 11298671
1366 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 726 bp
ATGGAGCTTCAGTTGGGTCTGGCTCTAGCTCTTCCTGACCATCACAGTCCTGTCAAAAGGGTTGACATGAATGTCGTTAATTGCAGCATCGAGAAAAAAGAGATGGTGGGTTTGGAGAGCAAAAACCAAGTTGGGAATTACAAGCGTAGTTTTGATGTGGCTTTTGAGAAGAGTAGCAGTACTGGTGCTGGAAATGAATCGAAGATGTTGGCTTTGTTGTTGTGGAATGATCAGCCAAATGAGGAAGATGAAGATGATAAGGGGCGAAAGAGAAGAAACTCTTGCAGGTCCATAATCAAGAATGATGATGGAGGAAATCATGTAGTTGGTTGGCCACCAATTAAATCATGGAGGAAAAAGATGCATCATGATCAACACCATCAGTATTACCCTCTCCAGAATAATCAGATGGCTAATAACTATAAGGAAAACGAAAACGATGGCTCAGCAGCAGCAGCGAATAATTCCATGTACGTAAAGGTGAAGATGGAAGGAGAGGGAATTGTGAGGAAGATCGACATAAATCTGCATAATTCTTTTCAGATGCTCAGAGACACCTTGATTACCATGTTTTCCATATGCAAAAGCAAAGAGGGTGGTGCTGCAGCTGATTATATACTCATTTATCAAGACAAACAAGGAGATTGGCTGCTCGCTGAAGATGTTCCCTGGCAAACCTTCATCGAGTCCGTGCAGCGCCTGCAGATAGTAAGGAATGGTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

242

Amino Acids

27.44

Weight (kDa)

6.45

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 7 - 239 4.2e-34 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
Acc36I ACCTGC 1 cut(s) 276
AcoI YGGCCR 1 cut(s) 332
AcuI CTGAAG 1 cut(s) 678
AfaI GTAC 2 cut(s) 181, 473
AjnI CCWGG 1 cut(s) 668
AluBI AGCT 3 cut(s) 7, 29, 608
AluI AGCT 3 cut(s) 7, 29, 608
Alw26I GTCTC 1 cut(s) 546
AlwNI CAGNNNCTG 1 cut(s) 700
AoxI GGCC 1 cut(s) 332
ApeKI GCWGC 8 cut(s) 84, 449, 452, 455, 602, 605, 649, 694
Asp700I GAANNNNTTC 1 cut(s) 663
AspLEI GCGC 1 cut(s) 699
AspS9I GGNCC 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 493
AvaII GGWCC 1 cut(s) 288
BalI TGGCCA 1 cut(s) 334
BbvI GCAGC 8 cut(s) 96, 461, 464, 467, 589, 617, 636, 706
BccI CCATC 7 cut(s) 48, 97, 302, 387, 403, 434, 481
BciT130I CCWGG 1 cut(s) 670
BclI TGATCA 2 cut(s) 229, 370
BcoDI GTCTC 1 cut(s) 546
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 2 cut(s) 603, 701
BfoI RGCGCY 1 cut(s) 700
BfuAI ACCTGC 1 cut(s) 276
BisI GCNGC 8 cut(s) 85, 450, 453, 456, 603, 606, 650, 695
BlpI GCTNAGC 1 cut(s) 445
BlsI GCNGC 8 cut(s) 86, 451, 454, 457, 604, 607, 651, 696
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 1 cut(s) 670
Bme18I GGWCC 1 cut(s) 288
BmgT120I GGNCC 1 cut(s) 288
BmrFI CCNGG 1 cut(s) 670
BmsI GCATC 4 cut(s) 96, 351, 373, 534
BpmI CTGGAG 1 cut(s) 380
Bpu1102I GCTNAGC 1 cut(s) 445
BsaAI YACGTR 1 cut(s) 475
BsaJI CCNNGG 1 cut(s) 668
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 670
BseDI CCNNGG 1 cut(s) 668
BseMII CTCAG 2 cut(s) 459, 562
BseNI ACTGG 1 cut(s) 187
BseXI GCAGC 8 cut(s) 96, 461, 464, 467, 589, 617, 636, 706
BsgI GTGCAG 1 cut(s) 713
BshFI GGCC 1 cut(s) 334
BsmAI GTCTC 1 cut(s) 546
BsnI GGCC 1 cut(s) 334
Bsp143I GATC 3 cut(s) 229, 370, 513
Bsp1720I GCTNAGC 1 cut(s) 445
BspANI GGCC 1 cut(s) 334
BspCNI CTCAG 2 cut(s) 458, 561
BspHI TCATGA 1 cut(s) 367
BspMAI CTGCAG 2 cut(s) 607, 705
BspMI ACCTGC 1 cut(s) 276
BspQI GCTCTTC 1 cut(s) 36
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 668
BssMI GATC 3 cut(s) 229, 370, 513
Bst2UI CCWGG 1 cut(s) 670
Bst4CI ACNGT 1 cut(s) 47
Bst6I CTCTTC 2 cut(s) 36, 164
BstAPI GCANNNNNTGC 1 cut(s) 700
BstBAI YACGTR 1 cut(s) 475
BstC8I GCNNGC 2 cut(s) 654, 701
BstDEI CTNAG 2 cut(s) 445, 548
BstH2I RGCGCY 1 cut(s) 700
BstHHI GCGC 1 cut(s) 699
BstKTI GATC 3 cut(s) 232, 373, 516
BstMAI GTCTC 1 cut(s) 546
BstMBI GATC 3 cut(s) 229, 370, 513
BstMWI GCNNNNNNNGC 2 cut(s) 455, 700
BstNI CCWGG 1 cut(s) 670
BstSCI CCNGG 1 cut(s) 668
BstSFI CTRYAG 2 cut(s) 603, 701
BstSNI TACGTA 1 cut(s) 475
BstV1I GCAGC 8 cut(s) 96, 461, 464, 467, 589, 617, 636, 706
BsuRI GGCC 1 cut(s) 334
BveI ACCTGC 1 cut(s) 276
Cac8I GCNNGC 2 cut(s) 654, 701
CaiI CAGNNNCTG 1 cut(s) 700
CciI TCATGA 1 cut(s) 367
CfoI GCGC 1 cut(s) 699
Cfr13I GGNCC 1 cut(s) 288
Csp6I GTAC 2 cut(s) 180, 472
CspCI CAANNNNNGTGG 2 cut(s) 577, 612
CviAII CATG 6 cut(s) 67, 320, 348, 368, 469, 568
CviQI GTAC 2 cut(s) 180, 472
DdeI CTNAG 2 cut(s) 445, 548
DpnI GATC 3 cut(s) 231, 372, 515
DpnII GATC 3 cut(s) 229, 370, 513
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 332
Eam1104I CTCTTC 2 cut(s) 36, 164
EarI CTCTTC 2 cut(s) 36, 164
Eco105I TACGTA 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 288
Eco57I CTGAAG 1 cut(s) 678
EcoRII CCWGG 1 cut(s) 668
EcoT22I ATGCAT 1 cut(s) 366
FaeI CATG 6 cut(s) 70, 323, 351, 371, 472, 571
FalI AAGNNNNNCTT 2 cut(s) 265, 297
FatI CATG 6 cut(s) 66, 319, 347, 367, 468, 567
FauNDI CATATG 1 cut(s) 578
FbaI TGATCA 2 cut(s) 229, 370
Fnu4HI GCNGC 8 cut(s) 85, 450, 453, 456, 603, 606, 650, 695
Fsp4HI GCNGC 8 cut(s) 85, 450, 453, 456, 603, 606, 650, 695
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 698
GluI GCNGC 8 cut(s) 85, 450, 453, 456, 603, 606, 650, 695
GsuI CTGGAG 1 cut(s) 380
HaeII RGCGCY 1 cut(s) 700
HaeIII GGCC 1 cut(s) 334
HhaI GCGC 1 cut(s) 699
Hin1II CATG 6 cut(s) 70, 323, 351, 371, 472, 571
Hin6I GCGC 1 cut(s) 697
HinP1I GCGC 1 cut(s) 697
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 2 cut(s) 197, 686
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 3 cut(s) 408, 543, 551
Hpy188III TCNNGA 6 cut(s) 35, 91, 298, 368, 397, 629
Hpy8I GTNNAC 1 cut(s) 64
HpyAV CCTTC 2 cut(s) 485, 688
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 8 cut(s) 84, 285, 364, 529, 582, 605, 694, 703
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 700
HpyF3I CTNAG 2 cut(s) 445, 548
HpySE526I ACGT 1 cut(s) 474
Hsp92II CATG 6 cut(s) 70, 323, 351, 371, 472, 571
HspAI GCGC 1 cut(s) 697
Ksp22I TGATCA 2 cut(s) 229, 370
Kzo9I GATC 3 cut(s) 229, 370, 513
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 8 cut(s) 96, 461, 464, 467, 589, 617, 636, 706
LweI GCATC 4 cut(s) 96, 351, 373, 534
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 474
MalI GATC 3 cut(s) 231, 372, 515
MboI GATC 3 cut(s) 229, 370, 513
MboII GAAGA 9 cut(s) 23, 181, 214, 257, 263, 285, 496, 523, 671
MlsI TGGCCA 1 cut(s) 334
MluCI AATT 6 cut(s) 79, 136, 339, 463, 501, 532
MluNI TGGCCA 1 cut(s) 334
MlyI GAGTC 1 cut(s) 695
MnlI CCTC 7 cut(s) 235, 305, 345, 402, 490, 501, 586
Mox20I TGGCCA 1 cut(s) 334
Mph1103I ATGCAT 1 cut(s) 366
MroXI GAANNNNTTC 1 cut(s) 663
MscI TGGCCA 1 cut(s) 334
MseI TTAA 2 cut(s) 78, 342
Msp20I TGGCCA 1 cut(s) 334
MspA1I CMGCKG 1 cut(s) 608
MspR9I CCNGG 1 cut(s) 670
MvaI CCWGG 1 cut(s) 670
MwoI GCNNNNNNNGC 2 cut(s) 455, 700
NdeI CATATG 1 cut(s) 578
NdeII GATC 3 cut(s) 229, 370, 513
NlaIII CATG 6 cut(s) 70, 323, 351, 371, 472, 571
NsiI ATGCAT 1 cut(s) 366
PagI TCATGA 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 663
PfeI GAWTC 1 cut(s) 197
PkrI GCNGC 8 cut(s) 86, 451, 454, 457, 604, 607, 651, 696
PleI GAGTC 1 cut(s) 694
PpsI GAGTC 1 cut(s) 694
Ppu21I YACGTR 1 cut(s) 475
Psp6I CCWGG 1 cut(s) 668
PspGI CCWGG 1 cut(s) 668
PspPI GGNCC 1 cut(s) 288
PstI CTGCAG 2 cut(s) 607, 705
PstNI CAGNNNCTG 1 cut(s) 700
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 2 cut(s) 181, 473
RsaNI GTAC 2 cut(s) 180, 472
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 2 cut(s) 78, 342
SatI GCNGC 8 cut(s) 85, 450, 453, 456, 603, 606, 650, 695
Sau3AI GATC 3 cut(s) 229, 370, 513
Sau96I GGNCC 1 cut(s) 288
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 1 cut(s) 695
ScrFI CCNGG 1 cut(s) 670
SetI ASST 8 cut(s) 9, 31, 290, 477, 483, 560, 610, 680
SfaNI GCATC 4 cut(s) 96, 351, 373, 534
SfcI CTRYAG 2 cut(s) 603, 701
SinI GGWCC 1 cut(s) 288
SnaBI TACGTA 1 cut(s) 475
Sse9I AATT 6 cut(s) 79, 136, 339, 463, 501, 532
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 668
TaaI ACNGT 1 cut(s) 47
TaiI ACGT 1 cut(s) 477
TaqI TCGA 4 cut(s) 90, 200, 516, 684
TasI AATT 6 cut(s) 79, 136, 339, 463, 501, 532
TatI WGTACW 1 cut(s) 179
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 2 cut(s) 78, 342
Tru9I TTAA 2 cut(s) 78, 342
TseI GCWGC 8 cut(s) 84, 449, 452, 455, 602, 605, 649, 694
TspDTI ATGAA 4 cut(s) 83, 210, 264, 670
TspGWI ACGGA 1 cut(s) 679
VpaK11BI GGWCC 1 cut(s) 288
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 663
XspI CTAG 1 cut(s) 26
ZrmI AGTACT 1 cut(s) 181
Zsp2I ATGCAT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.