pycom09g07010

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006955
Physical Location & Seq
Forward (+)
5182631 .. 5184287
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1158 bp
ATGTTTCTAAACTCAGTCCAACCCAAAGAGAAATTTAGGCTAAAAGCTAAATGGAGAAGTGAGGTCAGATTCCATAAAAAATTTAGCCCAAAAGTGGCTGTGGGCCCCACCGAAATGTTTGCTTTTCAGCGTTTGGGCGCCAAACACAGCCCAACCCAACCCCCAACGTGCCTAACGCGCGAGTCTTCCGACCGCGAGGGACCAACCCACATGGACCTGTCGGCCACATTCCGCCTTTTTTCTGAGAATTACAGACTCTACTCACGCAGTCACTTTGTGAAAGGGTTGGAATTGCTGATTCTGCTGATTGTTTATGGAGTCTATGGGGAATCTCACAGGAGTTCAAATCTTTTTCGGTTCATCATTTTCTCCATGTGGTTTCTCGTTGCTTCGTGGTTGTTTTCTCCTTCCATATTCAATCCGTCCAACTTTGACTGGCAGAAAATTGTAGATGACTGGACAGATTGGAAGAGGTGGATGGGAAACCGTGGTGGTATTGGGATATCACTTGAAAAAAGTTGGGAATCATGGTGGTATGAAGAACAGGAACACCTCAAATACACAGATTTCCGGGGAAGAATTCTTGAGATTATACTTGCGCTTCGGTTCCTTATTTACCAATATGGAATTGTTTACCACCTTGATATAGCTCATCACAGCAAGAGTTTACGGATAGTTTCCCTGGGTGGACGAAGATTTGGCACCGACTTTCAGCTCAGTTTCAGGATTTTGAAAGCGTTTCTGCTTCTTGGTTTCATGTCTGTCATGATTGTCCTATTTGTTGTTTGCGGCCTTACCATTTCAGATCTATTTGCTGCAATCCCTGCCTTTCTACCCACAGGCTGGGCCCTTCTTCTGTGGATCGATCGGACAATCATGCAGGCCGTTGTTCAAAGGGCTAGGACTCTGGGAATCAATCAAGGAGCTTGGAAGAGCATACGATTACATAATGGGACTAGTAATCTTCATGTCGATAGCCATTTTGTCATGGTTCCCGTTTATATCAGAGTTCCAAACACGTATCCTCTTCAACCAAGCAGTCAGCAGAGGCCTGCAGATTTCCAGGATTCTTCCAGGAAGGAAAGACTGCTACAGAAACTTAAAAATTGCCATCTGACATGGAAGCTTTTAGCATCTCAACTTCATATGCGCGATTAG

Protein Analysis

386

Amino Acids

45.05

Weight (kDa)

9.97

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glucan_synthase PF02364 78 - 195 6.5e-23 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 137, 701
AccB7I CCANNNNNTGG 1 cut(s) 843
AccII CGCG 4 cut(s) 178, 180, 195, 1152
AciI CCGC 3 cut(s) 193, 232, 789
AclWI GGATC 1 cut(s) 869
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 3 cut(s) 32, 80, 579
AcyI GRCGYC 1 cut(s) 138
AdeI CACNNNGTG 1 cut(s) 277
AfiI CCNNNNNNNGG 2 cut(s) 94, 843
AflIII ACRYGT 1 cut(s) 1017
AgsI TTSAA 6 cut(s) 345, 418, 512, 733, 893, 1031
AhlI ACTAGT 1 cut(s) 956
AjnI CCWGG 3 cut(s) 681, 1062, 1073
AluBI AGCT 5 cut(s) 47, 650, 715, 926, 1126
AluI AGCT 5 cut(s) 47, 650, 715, 926, 1126
AlwI GGATC 1 cut(s) 869
AoxI GGCC 6 cut(s) 103, 222, 790, 846, 882, 1049
ApaI GGGCCC 2 cut(s) 107, 850
ApeKI GCWGC 1 cut(s) 815
ApoI RAATTY 3 cut(s) 32, 80, 579
AspLEI GCGC 4 cut(s) 140, 180, 601, 1152
AspS9I GGNCC 6 cut(s) 103, 104, 200, 214, 846, 847
AsuC2I CCSGG 1 cut(s) 572
AvaII GGWCC 2 cut(s) 200, 214
BaeGI GKGCMC 2 cut(s) 107, 850
BanI GGYRCC 2 cut(s) 137, 701
BanII GRGCYC 2 cut(s) 107, 850
BbsI GAAGAC 1 cut(s) 177
BbvI GCAGC 1 cut(s) 802
BccI CCATC 2 cut(s) 472, 1119
BceAI ACGGC 1 cut(s) 869
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 3 cut(s) 683, 1064, 1075
BciVI GTATCC 1 cut(s) 1032
BcnI CCSGG 1 cut(s) 572
BcuI ACTAGT 1 cut(s) 956
BfaI CTAG 2 cut(s) 900, 957
BfmI CTRYAG 2 cut(s) 1053, 1091
BfoI RGCGCY 1 cut(s) 141
BfuI GTATCC 1 cut(s) 1032
BglII AGATCT 1 cut(s) 805
BisI GCNGC 2 cut(s) 790, 816
BlsI GCNGC 2 cut(s) 791, 817
Bme1390I CCNGG 4 cut(s) 572, 683, 1064, 1075
Bme18I GGWCC 2 cut(s) 200, 214
BmgT120I GGNCC 6 cut(s) 103, 104, 200, 214, 846, 847
BmiI GGNNCC 8 cut(s) 105, 106, 139, 201, 608, 703, 848, 993
BmrFI CCNGG 4 cut(s) 572, 683, 1064, 1075
BmsI GCATC 1 cut(s) 1142
BpiI GAAGAC 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 572
Bsa29I ATCGAT 1 cut(s) 864
BsaAI YACGTR 1 cut(s) 1020
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 4 cut(s) 487, 571, 681, 682
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 843
Bse1I ACTGG 2 cut(s) 440, 461
BseBI CCWGG 3 cut(s) 683, 1064, 1075
BseCI ATCGAT 1 cut(s) 864
BseDI CCNNGG 4 cut(s) 487, 571, 681, 682
BseGI GGATG 1 cut(s) 483
BseLI CCNNNNNNNGG 2 cut(s) 94, 843
BseMII CTCAG 3 cut(s) 27, 234, 730
BseNI ACTGG 2 cut(s) 440, 461
BseSI GKGCMC 2 cut(s) 107, 850
BseXI GCAGC 1 cut(s) 802
BseYI CCCAGC 1 cut(s) 843
Bsh1236I CGCG 4 cut(s) 178, 180, 195, 1152
Bsh1285I CGRYCG 2 cut(s) 193, 868
BshFI GGCC 6 cut(s) 105, 224, 792, 848, 884, 1051
BshNI GGYRCC 2 cut(s) 137, 701
BshVI ATCGAT 1 cut(s) 864
BsiEI CGRYCG 2 cut(s) 193, 868
BsiSI CCGG 1 cut(s) 571
BslFI GGGAC 2 cut(s) 213, 967
BslI CCNNNNNNNGG 2 cut(s) 94, 843
BsmFI GGGAC 2 cut(s) 213, 967
BsnI GGCC 6 cut(s) 105, 224, 792, 848, 884, 1051
Bsp120I GGGCCC 2 cut(s) 103, 846
Bsp1286I GDGCHC 2 cut(s) 107, 850
Bsp143I GATC 3 cut(s) 805, 861, 865
BspACI CCGC 3 cut(s) 193, 232, 789
BspANI GGCC 6 cut(s) 105, 224, 792, 848, 884, 1051
BspCNI CTCAG 3 cut(s) 26, 235, 729
BspDI ATCGAT 1 cut(s) 864
BspFNI CGCG 4 cut(s) 178, 180, 195, 1152
BspHI TCATGA 1 cut(s) 765
BspLI GGNNCC 8 cut(s) 105, 106, 139, 201, 608, 703, 848, 993
BspMAI CTGCAG 1 cut(s) 1057
BspPI GGATC 1 cut(s) 869
BspQI GCTCTTC 1 cut(s) 926
BspT107I GGYRCC 2 cut(s) 137, 701
BsrI ACTGG 2 cut(s) 440, 461
BssECI CCNNGG 4 cut(s) 487, 571, 681, 682
BssMI GATC 3 cut(s) 805, 861, 865
BssNI GRCGYC 1 cut(s) 138
Bst2UI CCWGG 3 cut(s) 683, 1064, 1075
Bst4CI ACNGT 1 cut(s) 488
Bst6I CTCTTC 3 cut(s) 464, 926, 1032
BstACI GRCGYC 1 cut(s) 138
BstAPI GCANNNNNTGC 1 cut(s) 824
BstBAI YACGTR 1 cut(s) 1020
BstC8I GCNNGC 2 cut(s) 882, 1053
BstDEI CTNAG 3 cut(s) 13, 243, 716
BstDSI CCRYGG 1 cut(s) 487
BstF5I GGATG 1 cut(s) 483
BstFNI CGCG 4 cut(s) 178, 180, 195, 1152
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 4 cut(s) 140, 180, 601, 1152
BstKTI GATC 3 cut(s) 808, 864, 868
BstMBI GATC 3 cut(s) 805, 861, 865
BstMCI CGRYCG 2 cut(s) 193, 868
BstMWI GCNNNNNNNGC 3 cut(s) 177, 301, 824
BstNI CCWGG 3 cut(s) 683, 1064, 1075
BstSCI CCNGG 4 cut(s) 570, 681, 1062, 1073
BstSFI CTRYAG 2 cut(s) 1053, 1091
BstSLI GKGCMC 2 cut(s) 107, 850
BstUI CGCG 4 cut(s) 178, 180, 195, 1152
BstV1I GCAGC 1 cut(s) 802
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
Bsu15I ATCGAT 1 cut(s) 864
BsuI GTATCC 1 cut(s) 1032
BsuRI GGCC 6 cut(s) 105, 224, 792, 848, 884, 1051
BsuTUI ATCGAT 1 cut(s) 864
BtgI CCRYGG 1 cut(s) 487
BtsCI GGATG 1 cut(s) 483
Cac8I GCNNGC 2 cut(s) 882, 1053
CciI TCATGA 1 cut(s) 765
CfoI GCGC 4 cut(s) 140, 180, 601, 1152
Cfr13I GGNCC 6 cut(s) 103, 104, 200, 214, 846, 847
ClaI ATCGAT 1 cut(s) 864
CviAII CATG 9 cut(s) 211, 373, 528, 757, 766, 877, 968, 988, 1119
DdeI CTNAG 3 cut(s) 13, 243, 716
DinI GGCGCC 1 cut(s) 139
DpnI GATC 3 cut(s) 807, 863, 867
DpnII GATC 3 cut(s) 805, 861, 865
DraIII CACNNNGTG 1 cut(s) 277
EaeI YGGCCR 1 cut(s) 222
Eam1104I CTCTTC 3 cut(s) 464, 926, 1032
EarI CTCTTC 3 cut(s) 464, 926, 1032
EciI GGCGGA 1 cut(s) 221
Eco147I AGGCCT 1 cut(s) 1051
Eco24I GRGCYC 2 cut(s) 107, 850
Eco32I GATATC 1 cut(s) 504
Eco47I GGWCC 2 cut(s) 200, 214
EcoO109I RGGNCCY 2 cut(s) 104, 847
EcoRI GAATTC 1 cut(s) 579
EcoRII CCWGG 3 cut(s) 681, 1062, 1073
EcoRV GATATC 1 cut(s) 504
EcoT38I GRGCYC 2 cut(s) 107, 850
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
FaeI CATG 9 cut(s) 214, 376, 531, 760, 769, 880, 971, 991, 1122
FaqI GGGAC 2 cut(s) 213, 967
FatI CATG 9 cut(s) 210, 372, 527, 756, 765, 876, 967, 987, 1118
FauNDI CATATG 1 cut(s) 1146
Fnu4HI GCNGC 2 cut(s) 790, 816
FokI GGATG 1 cut(s) 490
FriOI GRGCYC 2 cut(s) 107, 850
Fsp4HI GCNGC 2 cut(s) 790, 816
FspBI CTAG 2 cut(s) 900, 957
GlaI GCGC 4 cut(s) 139, 179, 600, 1151
GluI GCNGC 2 cut(s) 790, 816
GsaI CCCAGC 1 cut(s) 847
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 6 cut(s) 105, 224, 792, 848, 884, 1051
HapII CCGG 1 cut(s) 571
HhaI GCGC 4 cut(s) 140, 180, 601, 1152
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 9 cut(s) 214, 376, 531, 760, 769, 880, 971, 991, 1122
Hin6I GCGC 4 cut(s) 138, 178, 599, 1150
HinP1I GCGC 4 cut(s) 138, 178, 599, 1150
HindIII AAGCTT 1 cut(s) 1124
HpaII CCGG 1 cut(s) 571
Hpy166II GTNNAC 3 cut(s) 634, 668, 689
Hpy188I TCNGA 7 cut(s) 68, 190, 244, 805, 870, 1007, 1116
Hpy188III TCNNGA 3 cut(s) 584, 724, 766
Hpy8I GTNNAC 3 cut(s) 634, 668, 689
HpyAV CCTTC 3 cut(s) 417, 860, 1072
HpyCH4III ACNGT 1 cut(s) 488
HpyCH4IV ACGT 2 cut(s) 167, 1019
HpyCH4V TGCA 3 cut(s) 818, 880, 1055
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 301, 824
HpyF3I CTNAG 3 cut(s) 13, 243, 716
HpySE526I ACGT 2 cut(s) 167, 1019
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 9 cut(s) 214, 376, 531, 760, 769, 880, 971, 991, 1122
HspAI GCGC 4 cut(s) 138, 178, 599, 1150
KasI GGCGCC 1 cut(s) 137
Kzo9I GATC 3 cut(s) 805, 861, 865
LguI GCTCTTC 1 cut(s) 926
LmnI GCTCC 1 cut(s) 923
Lsp1109I GCAGC 1 cut(s) 802
LweI GCATC 1 cut(s) 1142
MaeI CTAG 2 cut(s) 900, 957
MaeII ACGT 2 cut(s) 167, 1019
MaeIII GTNAC 1 cut(s) 269
MalI GATC 3 cut(s) 807, 863, 867
MboI GATC 3 cut(s) 805, 861, 865
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 2 cut(s) 107, 850
MluCI AATT 8 cut(s) 32, 80, 247, 290, 444, 579, 627, 1105
Mly113I GGCGCC 1 cut(s) 138
MlyI GAGTC 4 cut(s) 191, 249, 327, 898
MmeI TCCRAC 4 cut(s) 43, 213, 267, 450
MnlI CCTC 6 cut(s) 55, 190, 465, 563, 1035, 1041
MseI TTAA 1 cut(s) 1101
MslI CAYNNNNRTG 1 cut(s) 113
MspI CCGG 1 cut(s) 571
MspR9I CCNGG 4 cut(s) 572, 683, 1064, 1075
MvaI CCWGG 3 cut(s) 683, 1064, 1075
MvnI CGCG 4 cut(s) 178, 180, 195, 1152
MwoI GCNNNNNNNGC 3 cut(s) 177, 301, 824
NarI GGCGCC 1 cut(s) 138
NciI CCSGG 1 cut(s) 572
NdeI CATATG 1 cut(s) 1146
NdeII GATC 3 cut(s) 805, 861, 865
NlaIII CATG 9 cut(s) 214, 376, 531, 760, 769, 880, 971, 991, 1122
NlaIV GGNNCC 8 cut(s) 105, 106, 139, 201, 608, 703, 848, 993
NmuCI GTSAC 1 cut(s) 269
PagI TCATGA 1 cut(s) 765
PasI CCCWGGG 1 cut(s) 682
PceI AGGCCT 1 cut(s) 1051
PciSI GCTCTTC 1 cut(s) 926
PfeI GAWTC 6 cut(s) 69, 298, 329, 524, 912, 1067
PflMI CCANNNNNTGG 1 cut(s) 843
PfoI TCCNGGA 2 cut(s) 1062, 1073
PkrI GCNGC 2 cut(s) 791, 817
Ple19I CGATCG 1 cut(s) 868
PleI GAGTC 4 cut(s) 190, 249, 326, 898
PluTI GGCGCC 1 cut(s) 141
PpsI GAGTC 4 cut(s) 190, 249, 326, 898
Ppu21I YACGTR 1 cut(s) 1020
Psp6I CCWGG 3 cut(s) 681, 1062, 1073
PspFI CCCAGC 1 cut(s) 843
PspGI CCWGG 3 cut(s) 681, 1062, 1073
PspN4I GGNNCC 8 cut(s) 105, 106, 139, 201, 608, 703, 848, 993
PspOMI GGGCCC 2 cut(s) 103, 846
PspPI GGNCC 6 cut(s) 103, 104, 200, 214, 846, 847
PstI CTGCAG 1 cut(s) 1057
PsuI RGATCY 1 cut(s) 805
PvuI CGATCG 1 cut(s) 868
RseI CAYNNNNRTG 1 cut(s) 113
SapI GCTCTTC 1 cut(s) 926
SaqAI TTAA 1 cut(s) 1101
SatI GCNGC 2 cut(s) 790, 816
Sau3AI GATC 3 cut(s) 805, 861, 865
Sau96I GGNCC 6 cut(s) 103, 104, 200, 214, 846, 847
SchI GAGTC 4 cut(s) 191, 249, 327, 898
ScrFI CCNGG 4 cut(s) 572, 683, 1064, 1075
SduI GDGCHC 2 cut(s) 107, 850
SfaNI GCATC 1 cut(s) 1142
SfcI CTRYAG 2 cut(s) 1053, 1091
SfoI GGCGCC 1 cut(s) 139
SinI GGWCC 2 cut(s) 200, 214
SmiMI CAYNNNNRTG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
SpeI ACTAGT 1 cut(s) 956
Sse9I AATT 8 cut(s) 32, 80, 247, 290, 444, 579, 627, 1105
SseBI AGGCCT 1 cut(s) 1051
SsiI CCGC 3 cut(s) 193, 232, 789
SspDI GGCGCC 1 cut(s) 137
SspMI CTAG 2 cut(s) 900, 957
StuI AGGCCT 1 cut(s) 1051
StyD4I CCNGG 4 cut(s) 570, 681, 1062, 1073
TaaI ACNGT 1 cut(s) 488
TaiI ACGT 2 cut(s) 170, 1022
TaqI TCGA 2 cut(s) 864, 972
TasI AATT 8 cut(s) 32, 80, 247, 290, 444, 579, 627, 1105
TauI GCSGC 1 cut(s) 792
TfiI GAWTC 6 cut(s) 69, 298, 329, 524, 912, 1067
Tru1I TTAA 1 cut(s) 1101
Tru9I TTAA 1 cut(s) 1101
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 1 cut(s) 815
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 5 cut(s) 349, 552, 745, 956, 1133
TspGWI ACGGA 2 cut(s) 411, 685
Van91I CCANNNNNTGG 1 cut(s) 843
VpaK11BI GGWCC 2 cut(s) 200, 214
XapI RAATTY 3 cut(s) 32, 80, 579
XspI CTAG 2 cut(s) 900, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.