pycom09g08240

Eukaryotic family of unknown function (DUF1754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002762
Physical Location & Seq
Forward (+)
6316330 .. 6318159
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
ATGACTATTGTATCAGTCCGAAATTTAAAAATTGGGTCGGGCCATCTGAAAGCGGGTTTACAATATAACAGCAAAGAAGACCTAGGACTCTTCGGGGTCCATATCGAACACAAAATCTTTAATCTTGGGTGTATCGTAAAACTTATGATAAAACATTCTTCTGTGAAATTCCAAAGCGATCGAGATCAAGCACTCTTTCTCTTTTGTTTCAGAGACAAAAACATGTCGTCGTATGAGAATGTCGTTGGTGGGAAGCTGAAGCTCAAGGGAAAGGCTCTGGATGTGAAGGCTGCTGGAATGAAGAAGAAAAAGAAGCATAAGAAACATCAAGAACAAACTTCTGTTGTAACGGAAACTGAGCTCTCACCTGATGAAAGTACAGAAGTAACCAATCCCGATGAGGAAGAAATAAACGATTCGCACAAATCAAGTGAGGAGGGGAAGGCTCCTCACTATGGTGATCTTCTCACACCTGCCGAGAGACGATATATAGAACAGAGAGAGAGAATTGATAATCACAGGCTGGCCAAGACAGCAAACAAGTCCCACCGTGACAGAATTCAAGACTTCAACCAATATCTGGCAAACATGAGCGAGCATTATGACATTCCTAAAGTCGGGCCAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1754

Protein Analysis

210

Amino Acids

23.79

Weight (kDa)

9.03

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM32A PF08555 78 - 172 1.4e-16 FAM32A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 481
Acc36I ACCTGC 1 cut(s) 481
AccB7I CCANNNNNTGG 1 cut(s) 580
AciI CCGC 1 cut(s) 53
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 3 cut(s) 22, 167, 558
AcuI CTGAAG 1 cut(s) 278
AfaI GTAC 1 cut(s) 379
AfiI CCNNNNNNNGG 3 cut(s) 455, 580, 617
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 2 cut(s) 563, 571
AjnI CCWGG 1 cut(s) 622
AleI CACNNNNGTG 1 cut(s) 456
AluBI AGCT 3 cut(s) 256, 262, 361
AluI AGCT 3 cut(s) 256, 262, 361
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 207, 475
AoxI GGCC 3 cut(s) 40, 525, 620
ApeKI GCWGC 1 cut(s) 290
ApoI RAATTY 3 cut(s) 22, 167, 558
AspA2I CCTAGG 1 cut(s) 82
AspS9I GGNCC 3 cut(s) 40, 97, 620
AsuHPI GGTGA 2 cut(s) 357, 470
AvaII GGWCC 1 cut(s) 97
AvrII CCTAGG 1 cut(s) 82
BaeI ACNNNNGTAYC 1 cut(s) 27
BalI TGGCCA 1 cut(s) 527
BanII GRGCYC 1 cut(s) 363
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 1 cut(s) 277
BccI CCATC 1 cut(s) 51
BciT130I CCWGG 1 cut(s) 624
BcoDI GTCTC 2 cut(s) 207, 475
BfaI CTAG 1 cut(s) 83
BfuAI ACCTGC 1 cut(s) 481
BisI GCNGC 1 cut(s) 291
BlnI CCTAGG 1 cut(s) 82
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 624
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 3 cut(s) 40, 97, 620
BmiI GGNNCC 2 cut(s) 98, 447
BmrFI CCNGG 1 cut(s) 624
BpiI GAAGAC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 248
BsaBI GATNNNNATC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 82
Bsc4I CCNNNNNNNGG 3 cut(s) 455, 580, 617
Bse8I GATNNNNATC 1 cut(s) 183
BseBI CCWGG 1 cut(s) 624
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 1 cut(s) 286
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 3 cut(s) 455, 580, 617
BseMII CTCAG 1 cut(s) 348
BseRI GAGGAG 2 cut(s) 438, 449
BseXI GCAGC 1 cut(s) 277
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 3 cut(s) 42, 527, 622
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 363
BslFI GGGAC 1 cut(s) 529
BslI CCNNNNNNNGG 3 cut(s) 455, 580, 617
BsmAI GTCTC 2 cut(s) 207, 475
BsmBI CGTCTC 1 cut(s) 475
BsmFI GGGAC 1 cut(s) 529
BsnI GGCC 3 cut(s) 42, 527, 622
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 3 cut(s) 178, 184, 460
BspACI CCGC 1 cut(s) 53
BspANI GGCC 3 cut(s) 42, 527, 622
BspCNI CTCAG 1 cut(s) 349
BspLI GGNNCC 2 cut(s) 98, 447
BspMI ACCTGC 1 cut(s) 481
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 3 cut(s) 178, 184, 460
BssT1I CCWWGG 1 cut(s) 82
Bst2UI CCWGG 1 cut(s) 624
Bst4CI ACNGT 1 cut(s) 551
Bst6I CTCTTC 1 cut(s) 95
BstC8I GCNNGC 2 cut(s) 525, 596
BstDEI CTNAG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 286
BstKTI GATC 3 cut(s) 181, 187, 463
BstMAI GTCTC 2 cut(s) 207, 475
BstMBI GATC 3 cut(s) 178, 184, 460
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstNI CCWGG 1 cut(s) 624
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 1 cut(s) 622
BstV1I GCAGC 1 cut(s) 277
BstV2I GAAGAC 1 cut(s) 84
BsuRI GGCC 3 cut(s) 42, 527, 622
BtsCI GGATG 1 cut(s) 286
BveI ACCTGC 1 cut(s) 481
Cac8I GCNNGC 2 cut(s) 525, 596
Cfr13I GGNCC 3 cut(s) 40, 97, 620
Csp6I GTAC 1 cut(s) 378
CviAII CATG 2 cut(s) 223, 589
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 1 cut(s) 357
DpnI GATC 3 cut(s) 180, 186, 462
DpnII GATC 3 cut(s) 178, 184, 460
DraI TTTAAA 1 cut(s) 27
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 361
EcoRI GAATTC 1 cut(s) 558
EcoRII CCWGG 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 82
Esp3I CGTCTC 1 cut(s) 475
FaeI CATG 2 cut(s) 226, 592
FaqI GGGAC 1 cut(s) 529
FatI CATG 2 cut(s) 222, 588
FauI CCCGC 1 cut(s) 46
Fnu4HI GCNGC 1 cut(s) 291
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 1 cut(s) 291
FspBI CTAG 1 cut(s) 83
GluI GCNGC 1 cut(s) 291
HaeIII GGCC 3 cut(s) 42, 527, 622
Hin1II CATG 2 cut(s) 226, 592
HinfI GANTC 2 cut(s) 87, 416
HphI GGTGA 2 cut(s) 357, 470
Hpy166II GTNNAC 1 cut(s) 59
Hpy188I TCNGA 3 cut(s) 20, 48, 212
Hpy188III TCNNGA 5 cut(s) 182, 278, 329, 395, 563
Hpy8I GTNNAC 1 cut(s) 59
Hpy99I CGWCG 1 cut(s) 232
HpyAV CCTTC 2 cut(s) 280, 436
HpyCH4III ACNGT 1 cut(s) 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
HpyF3I CTNAG 1 cut(s) 357
Hsp92II CATG 2 cut(s) 226, 592
Kzo9I GATC 3 cut(s) 178, 184, 460
LmnI GCTCC 1 cut(s) 451
LpnPI CCDG 8 cut(s) 263, 279, 381, 486, 505, 509, 566, 609
Lsp1109I GCAGC 1 cut(s) 277
MaeI CTAG 1 cut(s) 83
MaeIII GTNAC 3 cut(s) 346, 385, 551
MalI GATC 3 cut(s) 180, 186, 462
MboI GATC 3 cut(s) 178, 184, 460
MboII GAAGA 7 cut(s) 82, 89, 150, 313, 316, 416, 455
MhlI GDGCHC 1 cut(s) 363
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 5 cut(s) 22, 30, 167, 507, 558
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 1 cut(s) 81
MnlI CCTC 4 cut(s) 394, 427, 430, 459
Mox20I TGGCCA 1 cut(s) 527
MscI TGGCCA 1 cut(s) 527
MseI TTAA 2 cut(s) 26, 120
MslI CAYNNNNRTG 1 cut(s) 456
Msp20I TGGCCA 1 cut(s) 527
MspR9I CCNGG 1 cut(s) 624
MvaI CCWGG 1 cut(s) 624
MwoI GCNNNNNNNGC 1 cut(s) 533
NdeII GATC 3 cut(s) 178, 184, 460
NlaIII CATG 2 cut(s) 226, 592
NlaIV GGNNCC 2 cut(s) 98, 447
NmeAIII GCCGAG 1 cut(s) 502
NmuCI GTSAC 1 cut(s) 551
NspI RCATGY 1 cut(s) 226
OliI CACNNNNGTG 1 cut(s) 456
PaqCI CACCTGC 1 cut(s) 481
PciI ACATGT 1 cut(s) 222
PfeI GAWTC 1 cut(s) 416
PflMI CCANNNNNTGG 1 cut(s) 580
PkrI GCNGC 1 cut(s) 292
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
PscI ACATGT 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 622
PspGI CCWGG 1 cut(s) 622
PspN4I GGNNCC 2 cut(s) 98, 447
PspPI GGNCC 3 cut(s) 40, 97, 620
PvuI CGATCG 1 cut(s) 181
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
RseI CAYNNNNRTG 1 cut(s) 456
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 26, 120
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 3 cut(s) 178, 184, 460
Sau96I GGNCC 3 cut(s) 40, 97, 620
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 1 cut(s) 624
SduI GDGCHC 1 cut(s) 363
SetI ASST 6 cut(s) 84, 258, 264, 363, 370, 475
SinI GGWCC 1 cut(s) 97
SmiMI CAYNNNNRTG 1 cut(s) 456
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 5 cut(s) 22, 30, 167, 507, 558
SsiI CCGC 1 cut(s) 53
SspMI CTAG 1 cut(s) 83
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 1 cut(s) 622
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 551
TaqI TCGA 2 cut(s) 105, 181
TasI AATT 5 cut(s) 22, 30, 167, 507, 558
TatI WGTACW 1 cut(s) 377
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 2 cut(s) 26, 120
Tru9I TTAA 2 cut(s) 26, 120
TseFI GTSAC 1 cut(s) 551
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 551
TspDTI ATGAA 2 cut(s) 314, 387
TspGWI ACGGA 1 cut(s) 365
Van91I CCANNNNNTGG 1 cut(s) 580
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 3 cut(s) 22, 167, 558
XceI RCATGY 1 cut(s) 226
XmaJI CCTAGG 1 cut(s) 82
XspI CTAG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.