pycom09g11870

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002379
Physical Location & Seq
Reverse (-)
10052216 .. 10054115
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1224 bp
ATGGGGAGAGCTACAAAATGGTTGAAGGGCTTGCTGGGGATGAAGAGAGAGAAACACCACGTCGACAACACCAGCACCGTGTCCAGCGACCGGAAGGAGAAGAAAAGGTGGAGCTTTGCCAAGTCAGGGAAAGACATTAACACCAATCTTCCGCCGAATTTTCCTGTCCCTGCTGACTCTGCCTGGCTCAGATCCTATCTTGCCGAGTCGGAGAAGGAGCAGAACAAGCACGCAATTGCAGTGGCTGCGGCCACCGCAGCCGCTGCTGATGCCGCTGTGGCCGCCGCACAGGCGGCTGTGGCTGTTGTGAGGCTGACAAGTCAAGGCGGCCATGGCGGTGGAGGCGGGATCATGTTTGGGAAAAGAGAGAGATGGGCTGCAATGAAGATTCAAACAGTTTTCAGAGGCTATTTGGCGAGGAAGGCGCACCGTGCTCTTAGAGGACTGGTTAAGTTGCAGGCTCTTGTTAGAGGCTTTCTTGTCCGGAAACGGGCTGCTGCTACTCTTTACAGTATGCAAGCTCTTTTTAGAGCACAGACTACTGTCCGATACCAGCGTGCGCGTCGTTCTTTCAATAAAGAGAATAGGTTCCTACCCGAAATCAATGCCCGGAAGTCTGTTGAAAGATTTGAAGATGCAAGAAGTGAATTTTATAGCAGGTGGCTATCTGCAACTTATGAAACTTCGGTTGATAGCTTTGATGAAAGCCCGAAAATTGTGGAAATTGACACATTCAAGCCGAGATCCAGGTCTCGTAGATTCAACACCATATTATCTGATCGTGGCGAAGACCTGCATTACCAAACAATCTCCTCACCGCTGCCCTGCCCAATGCCAACTCGTATCTCTATACCTGATAGCCAGAAACCTCAAGATTTTGAATGGTACTTCAATGGTGATGAATGCAAGTTCTCGACTGCCCATAACACTCCACGGTTTGCCACCTCTTTTCGTTCTAATGCTCCCGGCACACCATCTAAAAGTGTTTGTGGAGACAGCTTCTTTCGGCCTTACTCAAACTTCCCAAACTACATGTCAAGCACTCAGTCATTTAATGCCAAGTCAAGATCCCACAGCGCCCCGAAGCAAAGGCCGGAACCAGGGCCTAAGAAGAGGCTTTCGCTTAACGAAATGATGGCAGCAAGGAACAGCATCTGCAGCATTCGAATGCATCGATCGTGCAATCAAGTCCAAGAAGAAGAAGAAGAAGAAGAAGAAGAATAA

Protein Analysis

408

Amino Acids

46.02

Weight (kDa)

9.98

Isoelectric Point (pI)

64.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 311 - 387 6.7e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 648
Acc36I ACCTGC 2 cut(s) 648, 801
AccI GTMKAC 1 cut(s) 63
AccII CGCG 1 cut(s) 562
AccIII TCCGGA 1 cut(s) 483
AclWI GGATC 4 cut(s) 186, 356, 738, 1062
AcoI YGGCCR 3 cut(s) 249, 279, 328
AcsI RAATTY 2 cut(s) 157, 647
AfaI GTAC 1 cut(s) 887
AfiI CCNNNNNNNGG 4 cut(s) 90, 126, 490, 1100
AflIII ACRYGT 1 cut(s) 1032
AgsI TTSAA 9 cut(s) 25, 392, 574, 623, 632, 736, 763, 881, 892
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 3 cut(s) 182, 746, 1099
AluBI AGCT 5 cut(s) 11, 114, 521, 696, 999
AluI AGCT 5 cut(s) 11, 114, 521, 696, 999
Alw21I GWGCWC 2 cut(s) 436, 535
Alw26I GTCTC 2 cut(s) 756, 987
AlwI GGATC 4 cut(s) 186, 356, 738, 1062
AlwNI CAGNNNCTG 3 cut(s) 245, 263, 1155
Aor13HI TCCGGA 1 cut(s) 483
AoxI GGCC 6 cut(s) 249, 279, 328, 1007, 1091, 1103
ApeKI GCWGC 9 cut(s) 245, 257, 263, 377, 494, 497, 820, 1139, 1158
ApoI RAATTY 2 cut(s) 157, 647
ArsI GACNNNNNNTTYG 2 cut(s) 1019, 1051
Asp700I GAANNNNTTC 1 cut(s) 587
AspLEI GCGC 3 cut(s) 427, 562, 1079
AspS9I GGNCC 1 cut(s) 1103
AsuC2I CCSGG 2 cut(s) 610, 966
AsuHPI GGTGA 2 cut(s) 807, 908
AsuII TTCGAA 1 cut(s) 1165
BaeI ACNNNNGTAYC 2 cut(s) 541, 574
BbsI GAAGAC 1 cut(s) 795
Bbv12I GWGCWC 2 cut(s) 436, 535
BbvI GCAGC 9 cut(s) 232, 250, 269, 364, 481, 484, 807, 1151, 1170
BccI CCATC 3 cut(s) 366, 982, 1129
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciT130I CCWGG 3 cut(s) 184, 748, 1101
BcnI CCSGG 2 cut(s) 610, 966
BcoDI GTCTC 2 cut(s) 756, 987
BfmI CTRYAG 1 cut(s) 1156
BfoI RGCGCY 1 cut(s) 1080
BfuAI ACCTGC 2 cut(s) 648, 801
BglI GCCNNNNNGGC 2 cut(s) 278, 290
Bme1390I CCNGG 5 cut(s) 184, 610, 748, 966, 1101
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 1103
BmiI GGNNCC 2 cut(s) 590, 1098
BmrFI CCNGG 5 cut(s) 184, 610, 748, 966, 1101
BmsI GCATC 4 cut(s) 259, 625, 1161, 1180
BoxI GACNNNNGTC 1 cut(s) 542
BpiI GAAGAC 1 cut(s) 795
Bpu14I TTCGAA 1 cut(s) 1165
BpuEI CTTGAG 1 cut(s) 855
BpuMI CCSGG 2 cut(s) 610, 966
Bsa29I ATCGAT 1 cut(s) 1174
BsaI GGTCTC 1 cut(s) 756
BsaJI CCNNGG 3 cut(s) 331, 932, 1100
BsaWI WCCGGW 2 cut(s) 90, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 126, 490, 1100
Bse1I ACTGG 1 cut(s) 450
Bse3DI GCAATG 1 cut(s) 387
BseAI TCCGGA 1 cut(s) 483
BseBI CCWGG 3 cut(s) 184, 748, 1101
BseCI ATCGAT 1 cut(s) 1174
BseDI CCNNGG 3 cut(s) 331, 932, 1100
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 4 cut(s) 90, 126, 490, 1100
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 202, 1058
BseNI ACTGG 1 cut(s) 450
BseRI GAGGAG 1 cut(s) 802
BseXI GCAGC 9 cut(s) 232, 250, 269, 364, 481, 484, 807, 1151, 1170
BseYI CCCAGC 1 cut(s) 34
Bsh1236I CGCG 1 cut(s) 562
Bsh1285I CGRYCG 2 cut(s) 91, 1178
BshFI GGCC 6 cut(s) 251, 281, 330, 1009, 1093, 1105
BshVI ATCGAT 1 cut(s) 1174
BsiEI CGRYCG 2 cut(s) 91, 1178
BsiHKAI GWGCWC 2 cut(s) 436, 535
BsiSI CCGG 5 cut(s) 91, 484, 610, 966, 1094
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 4 cut(s) 90, 126, 490, 1100
BsmAI GTCTC 2 cut(s) 756, 987
BsmFI GGGAC 1 cut(s) 152
BsmI GAATGC 3 cut(s) 908, 1161, 1173
BsnI GGCC 6 cut(s) 251, 281, 330, 1009, 1093, 1105
Bso31I GGTCTC 1 cut(s) 756
Bsp119I TTCGAA 1 cut(s) 1165
Bsp1286I GDGCHC 2 cut(s) 436, 535
Bsp13I TCCGGA 1 cut(s) 483
Bsp143I GATC 6 cut(s) 191, 348, 743, 778, 1067, 1175
Bsp19I CCATGG 1 cut(s) 331
BspANI GGCC 6 cut(s) 251, 281, 330, 1009, 1093, 1105
BspCNI CTCAG 2 cut(s) 201, 1057
BspDI ATCGAT 1 cut(s) 1174
BspEI TCCGGA 1 cut(s) 483
BspFNI CGCG 1 cut(s) 562
BspLI GGNNCC 2 cut(s) 590, 1098
BspMAI CTGCAG 1 cut(s) 1160
BspMI ACCTGC 2 cut(s) 648, 801
BspPI GGATC 4 cut(s) 186, 356, 738, 1062
BspT104I TTCGAA 1 cut(s) 1165
BspTNI GGTCTC 1 cut(s) 756
BsrDI GCAATG 1 cut(s) 387
BsrI ACTGG 1 cut(s) 450
BssECI CCNNGG 3 cut(s) 331, 932, 1100
BssMI GATC 6 cut(s) 191, 348, 743, 778, 1067, 1175
BssT1I CCWWGG 1 cut(s) 331
Bst2UI CCWGG 3 cut(s) 184, 748, 1101
Bst4CI ACNGT 6 cut(s) 79, 397, 431, 512, 544, 936
Bst6I CTCTTC 2 cut(s) 38, 1106
BstAPI GCANNNNNTGC 2 cut(s) 245, 263
BstBI TTCGAA 1 cut(s) 1165
BstC8I GCNNGC 5 cut(s) 32, 231, 459, 519, 558
BstDEI CTNAG 4 cut(s) 188, 437, 1044, 1107
BstDSI CCRYGG 2 cut(s) 331, 932
BstF5I GGATG 1 cut(s) 45
BstFNI CGCG 1 cut(s) 562
BstH2I RGCGCY 1 cut(s) 1080
BstHHI GCGC 3 cut(s) 427, 562, 1079
BstKTI GATC 6 cut(s) 194, 351, 746, 781, 1070, 1178
BstMAI GTCTC 2 cut(s) 756, 987
BstMBI GATC 6 cut(s) 191, 348, 743, 778, 1067, 1175
BstMCI CGRYCG 2 cut(s) 91, 1178
BstNI CCWGG 3 cut(s) 184, 748, 1101
BstNSI RCATGY 1 cut(s) 1036
BstPAI GACNNNNGTC 1 cut(s) 542
BstSCI CCNGG 5 cut(s) 182, 608, 746, 964, 1099
BstSFI CTRYAG 1 cut(s) 1156
BstUI CGCG 1 cut(s) 562
BstV1I GCAGC 9 cut(s) 232, 250, 269, 364, 481, 484, 807, 1151, 1170
BstV2I GAAGAC 1 cut(s) 795
BstX2I RGATCY 3 cut(s) 191, 743, 1067
BstXI CCANNNNNNTGG 1 cut(s) 338
BstYI RGATCY 3 cut(s) 191, 743, 1067
Bsu15I ATCGAT 1 cut(s) 1174
BsuRI GGCC 6 cut(s) 251, 281, 330, 1009, 1093, 1105
BsuTUI ATCGAT 1 cut(s) 1174
BtgI CCRYGG 2 cut(s) 331, 932
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 1 cut(s) 45
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 1 cut(s) 246
BveI ACCTGC 2 cut(s) 648, 801
Cac8I GCNNGC 5 cut(s) 32, 231, 459, 519, 558
CaiI CAGNNNCTG 3 cut(s) 245, 263, 1155
CfoI GCGC 3 cut(s) 427, 562, 1079
Cfr13I GGNCC 1 cut(s) 1103
ClaI ATCGAT 1 cut(s) 1174
CseI GACGC 1 cut(s) 551
Csp6I GTAC 1 cut(s) 886
CspCI CAANNNNNGTGG 2 cut(s) 222, 257
CviAII CATG 3 cut(s) 332, 352, 1033
CviQI GTAC 1 cut(s) 886
DdeI CTNAG 4 cut(s) 188, 437, 1044, 1107
DpnI GATC 6 cut(s) 193, 350, 745, 780, 1069, 1177
DpnII GATC 6 cut(s) 191, 348, 743, 778, 1067, 1175
EaeI YGGCCR 3 cut(s) 249, 279, 328
Eam1104I CTCTTC 2 cut(s) 38, 1106
EarI CTCTTC 2 cut(s) 38, 1106
EciI GGCGGA 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 331
Eco31I GGTCTC 1 cut(s) 756
EcoO109I RGGNCCY 1 cut(s) 1103
EcoRII CCWGG 3 cut(s) 182, 746, 1099
EcoT14I CCWWGG 1 cut(s) 331
EcoT22I ATGCAT 1 cut(s) 1173
ErhI CCWWGG 1 cut(s) 331
FaeI CATG 3 cut(s) 335, 355, 1036
FaiI YATR 9 cut(s) 333, 353, 515, 654, 678, 770, 851, 924, 1034
FaqI GGGAC 1 cut(s) 152
FatI CATG 3 cut(s) 331, 351, 1032
FauI CCCGC 1 cut(s) 338
FblI GTMKAC 1 cut(s) 63
FokI GGATG 1 cut(s) 52
GlaI GCGC 3 cut(s) 426, 561, 1078
GsaI CCCAGC 1 cut(s) 38
HaeII RGCGCY 1 cut(s) 1080
HaeIII GGCC 6 cut(s) 251, 281, 330, 1009, 1093, 1105
HapII CCGG 5 cut(s) 91, 484, 610, 966, 1094
HgaI GACGC 1 cut(s) 551
HhaI GCGC 3 cut(s) 427, 562, 1079
Hin1II CATG 3 cut(s) 335, 355, 1036
Hin6I GCGC 3 cut(s) 425, 560, 1077
HinP1I GCGC 3 cut(s) 425, 560, 1077
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 4 cut(s) 176, 206, 388, 759
HpaII CCGG 5 cut(s) 91, 484, 610, 966, 1094
HphI GGTGA 2 cut(s) 807, 908
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 5 cut(s) 191, 211, 404, 548, 778
Hpy188III TCNNGA 4 cut(s) 484, 872, 913, 1065
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 2 cut(s) 65, 567
HpyAV CCTTC 4 cut(s) 19, 88, 208, 415
HpyCH4III ACNGT 6 cut(s) 79, 397, 431, 512, 544, 936
HpyCH4IV ACGT 1 cut(s) 60
HpyF3I CTNAG 4 cut(s) 188, 437, 1044, 1107
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 3 cut(s) 335, 355, 1036
HspAI GCGC 3 cut(s) 425, 560, 1077
Kpn2I TCCGGA 1 cut(s) 483
Kzo9I GATC 6 cut(s) 191, 348, 743, 778, 1067, 1175
LmnI GCTCC 3 cut(s) 111, 217, 967
Lsp1109I GCAGC 9 cut(s) 232, 250, 269, 364, 481, 484, 807, 1151, 1170
LweI GCATC 4 cut(s) 259, 625, 1161, 1180
MaeII ACGT 1 cut(s) 60
MalI GATC 6 cut(s) 193, 350, 745, 780, 1069, 1177
MboI GATC 6 cut(s) 191, 348, 743, 778, 1067, 1175
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 3 cut(s) 191, 743, 1067
MhlI GDGCHC 2 cut(s) 436, 535
MluCI AATT 5 cut(s) 157, 234, 647, 714, 723
MlyI GAGTC 2 cut(s) 170, 215
MmeI TCCRAC 1 cut(s) 189
Mph1103I ATGCAT 1 cut(s) 1173
MroI TCCGGA 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 587
MseI TTAA 4 cut(s) 138, 450, 1053, 1125
MslI CAYNNNNRTG 2 cut(s) 336, 1166
MspA1I CMGCKG 3 cut(s) 263, 275, 820
MspI CCGG 5 cut(s) 91, 484, 610, 966, 1094
MspR9I CCNGG 5 cut(s) 184, 610, 748, 966, 1101
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 3 cut(s) 908, 1161, 1173
MvaI CCWGG 3 cut(s) 184, 748, 1101
MvnI CGCG 1 cut(s) 562
NciI CCSGG 2 cut(s) 610, 966
NcoI CCATGG 1 cut(s) 331
NdeII GATC 6 cut(s) 191, 348, 743, 778, 1067, 1175
NlaIII CATG 3 cut(s) 335, 355, 1036
NlaIV GGNNCC 2 cut(s) 590, 1098
NmeAIII GCCGAG 2 cut(s) 229, 765
NsiI ATGCAT 1 cut(s) 1173
NspI RCATGY 1 cut(s) 1036
NspV TTCGAA 1 cut(s) 1165
PaqCI CACCTGC 1 cut(s) 648
PciI ACATGT 1 cut(s) 1032
PcsI WCGNNNNNNNCGW 1 cut(s) 1171
PctI GAATGC 3 cut(s) 908, 1161, 1173
PdmI GAANNNNTTC 1 cut(s) 587
PfeI GAWTC 2 cut(s) 388, 759
Ple19I CGATCG 1 cut(s) 1178
PleI GAGTC 2 cut(s) 170, 214
PpsI GAGTC 2 cut(s) 170, 214
PscI ACATGT 1 cut(s) 1032
PshAI GACNNNNGTC 1 cut(s) 542
Psp6I CCWGG 3 cut(s) 182, 746, 1099
PspFI CCCAGC 1 cut(s) 34
PspGI CCWGG 3 cut(s) 182, 746, 1099
PspN4I GGNNCC 2 cut(s) 590, 1098
PspPI GGNCC 1 cut(s) 1103
PstI CTGCAG 1 cut(s) 1160
PstNI CAGNNNCTG 3 cut(s) 245, 263, 1155
PsuI RGATCY 3 cut(s) 191, 743, 1067
PvuI CGATCG 1 cut(s) 1178
RsaI GTAC 1 cut(s) 887
RsaNI GTAC 1 cut(s) 886
RseI CAYNNNNRTG 2 cut(s) 336, 1166
SalI GTCGAC 1 cut(s) 62
SaqAI TTAA 4 cut(s) 138, 450, 1053, 1125
Sau3AI GATC 6 cut(s) 191, 348, 743, 778, 1067, 1175
Sau96I GGNCC 1 cut(s) 1103
SchI GAGTC 2 cut(s) 170, 215
ScrFI CCNGG 5 cut(s) 184, 610, 748, 966, 1101
SduI GDGCHC 2 cut(s) 436, 535
SfaNI GCATC 4 cut(s) 259, 625, 1161, 1180
SfcI CTRYAG 1 cut(s) 1156
SfuI TTCGAA 1 cut(s) 1165
SmiMI CAYNNNNRTG 2 cut(s) 336, 1166
SmlI CTYRAG 1 cut(s) 870
SmoI CTYRAG 1 cut(s) 870
Sse9I AATT 5 cut(s) 157, 234, 647, 714, 723
StyD4I CCNGG 5 cut(s) 182, 608, 746, 964, 1099
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 6 cut(s) 79, 397, 431, 512, 544, 936
TaiI ACGT 1 cut(s) 63
TaqI TCGA 4 cut(s) 63, 914, 1165, 1174
TasI AATT 5 cut(s) 157, 234, 647, 714, 723
TauI GCSGC 7 cut(s) 251, 263, 275, 284, 287, 296, 330
TfiI GAWTC 2 cut(s) 388, 759
Tru1I TTAA 4 cut(s) 138, 450, 1053, 1125
Tru9I TTAA 4 cut(s) 138, 450, 1053, 1125
TscAI CASTG 1 cut(s) 246
TseI GCWGC 9 cut(s) 245, 257, 263, 377, 494, 497, 820, 1139, 1158
TspDTI ATGAA 5 cut(s) 56, 398, 693, 717, 915
TspRI CASTG 1 cut(s) 246
XapI RAATTY 2 cut(s) 157, 647
XceI RCATGY 1 cut(s) 1036
XmiI GTMKAC 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 587
Zsp2I ATGCAT 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.