pycom09g15200

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002221
Physical Location & Seq
Forward (+)
15053000 .. 15055811
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1311 bp
ATGGATGGTTCTCAGGGTGGTTCTGGTGGTGCGCCGGCGCCGTTTCTTACTAAAACATATGACTTGGTGGATGATCCTTCGTCCAACCACATGGTGTCTTGGAGTGAAACTGGTTCTAGTTTTGTAGTTTGGGATCCCACCGAGTTCGCCAAGGAAATGCTTCCCATGTATTTCAAGCACAACAATTTTTCGAGTTTCGTGAGGCAGCTTAATACATATGGGTTTAGAAAGATTGATCGAGAACAGTGGGAGTTTGCAAATGAGGAATTCTTAAGAGGAGGAAGACATCTGCTGAAGAATATTCACCGCCGCAAGCCAATTCATAGTCATTCCTTGCAGAATCACGAGTATTCTTCAGTTCCTTTGACTGATACGGAGAGAGAAGAATATGAGAAGAAAATCAACAGACTGAATCATGATAAAAGCTTGCTTGAGTTGGAGGTACAACGGCATCAAAGAGAGAACCAGGAGTTTGAATTTCAAATACAGATATTACGAGAACAGCTGCAGAACATGGAGAATCGGCAGAGGCAATATACGGCTTTCCTTGCTCAACTAGTGCAGAAACCGGGATTTGCTTCTGTACTTGTGCAGCAGTCAGAAATCCATAGCAAAAAGAGAAGATTACTGAAATCGAACGACTTTCCTGATGACTTTGGTATGGAAGATTTGACTTTGAATCCGCAGAAAGAAACTTTGGGAATAAAGTTGGACCAGCTTGAGAAGATGGAGTCTTCCTTGAACTTTTGGGAAGATTTTCTACATGGGGTTGGGGAAGCGATGCCCGAAGAATTAAATGATATTGGCCCGTTGTCTCAGGCCTCTCCTATCATTGTTACAGAAATACAAGACACCAGCATGAATAGCAGGCCTTGCTCACCTAGATCGCATATATCTTCACCGAATTCAATGAATGCTCATTCCTTCCCAGAGGTGGCTGGCTCTGCAAATCCTTTTGACATCCTTACTATAACATCAATGTGCCACACTGTTGATTTCAGGTCTAAATCTTCAGGAATTGATATGAACTCCAAGCCCGACAGTGCCCCTGCTCTTGATTCCTCGAAAGAAAATGTAGTAGAAGTGAAAAATGCCGAGCCTGCCAAGGCAAATGATGTCTTTTGGGAACAATGCCTAACAGAGACTCCTGGTTTAGATGATGCGCCGGAAGTACAGTCAGAAAGAATGGATGGCGATGGCGATGGCAAAGCGAGTGATGCCAATCCAGCTATTCGGAAGAAGCTTTGGTGGAACACAGATAATGTAGATAACTTTAGGAACCAAATAGGGCGCCTCACTCCAGCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04212 ko05134 map04212 map05134
Pfam Domains
Protein Families
HSF_DNA-bind

Protein Analysis

437

Amino Acids

49.66

Weight (kDa)

5.18

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 15 - 104 5.1e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 37, 1290
AciI CCGC 3 cut(s) 307, 310, 683
AclWI GGATC 3 cut(s) 68, 128, 141
AcsI RAATTY 3 cut(s) 266, 476, 904
AcuI CTGAAG 3 cut(s) 314, 339, 996
AcyI GRCGYC 2 cut(s) 38, 1291
AdeI CACNNNGTG 1 cut(s) 94
AfaI GTAC 3 cut(s) 444, 585, 1173
AfiI CCNNNNNNNGG 1 cut(s) 934
AflII CTTAAG 1 cut(s) 271
AgsI TTSAA 6 cut(s) 175, 476, 482, 679, 742, 909
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 2 cut(s) 465, 1147
AjuI GAANNNNNNNTTGG 2 cut(s) 680, 712
AluBI AGCT 7 cut(s) 208, 426, 505, 718, 1229, 1243, 1304
AluI AGCT 7 cut(s) 208, 426, 505, 718, 1229, 1243, 1304
Alw26I GTCTC 2 cut(s) 819, 1136
AlwI GGATC 3 cut(s) 68, 128, 141
AoxI GGCC 3 cut(s) 805, 819, 869
ApeKI GCWGC 3 cut(s) 205, 505, 592
ApoI RAATTY 3 cut(s) 266, 476, 904
Asp700I GAANNNNTTC 2 cut(s) 159, 756
AspLEI GCGC 4 cut(s) 34, 40, 1165, 1293
AspS9I GGNCC 2 cut(s) 712, 806
AsuC2I CCSGG 1 cut(s) 570
AsuHPI GGTGA 3 cut(s) 296, 870, 891
AvaII GGWCC 1 cut(s) 712
BaeGI GKGCMC 1 cut(s) 1048
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 2 cut(s) 37, 1290
BarI GAAGNNNNNNTAC 2 cut(s) 744, 776
BauI CACGAG 1 cut(s) 344
BbsI GAAGAC 2 cut(s) 289, 726
BbvI GCAGC 3 cut(s) 217, 492, 604
BccI CCATC 4 cut(s) 721, 1184, 1190, 1196
BceAI ACGGC 3 cut(s) 25, 464, 555
BciT130I CCWGG 2 cut(s) 467, 1149
BcnI CCSGG 1 cut(s) 570
BcoDI GTCTC 2 cut(s) 819, 1136
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 3 cut(s) 117, 557, 882
BfmI CTRYAG 1 cut(s) 506
BfoI RGCGCY 2 cut(s) 41, 1294
BfrI CTTAAG 1 cut(s) 271
BisI GCNGC 4 cut(s) 206, 310, 506, 593
BlsI GCNGC 4 cut(s) 207, 311, 507, 594
Bme1390I CCNGG 3 cut(s) 467, 570, 1149
Bme18I GGWCC 1 cut(s) 712
BmgT120I GGNCC 2 cut(s) 712, 806
BmiI GGNNCC 4 cut(s) 39, 135, 1280, 1292
BmrFI CCNGG 3 cut(s) 467, 570, 1149
BmsI GCATC 4 cut(s) 460, 771, 1150, 1207
BpiI GAAGAC 2 cut(s) 289, 726
BpmI CTGGAG 1 cut(s) 1284
BpuEI CTTGAG 2 cut(s) 452, 740
BpuMI CCSGG 1 cut(s) 570
BsaBI GATNNNNATC 1 cut(s) 1221
BsaHI GRCGYC 2 cut(s) 38, 1291
BsaJI CCNNGG 2 cut(s) 150, 1104
BsaXI ACNNNNNCTCC 2 cut(s) 1129, 1159
Bsc4I CCNNNNNNNGG 1 cut(s) 934
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 115
Bse8I GATNNNNATC 1 cut(s) 1221
BseBI CCWGG 2 cut(s) 467, 1149
BseDI CCNNGG 2 cut(s) 150, 1104
BseGI GGATG 4 cut(s) 10, 76, 960, 1195
BseJI GATNNNNATC 1 cut(s) 1221
BseLI CCNNNNNNNGG 1 cut(s) 934
BseMII CTCAG 2 cut(s) 26, 830
BseNI ACTGG 1 cut(s) 115
BseRI GAGGAG 1 cut(s) 291
BseSI GKGCMC 1 cut(s) 1048
BseXI GCAGC 3 cut(s) 217, 492, 604
BsgI GTGCAG 2 cut(s) 581, 611
BshFI GGCC 3 cut(s) 807, 821, 871
BshNI GGYRCC 2 cut(s) 37, 1290
BsiSI CCGG 3 cut(s) 35, 569, 1166
BslI CCNNNNNNNGG 1 cut(s) 934
BsmAI GTCTC 2 cut(s) 819, 1136
BsmI GAATGC 1 cut(s) 919
BsnI GGCC 3 cut(s) 807, 821, 871
Bsp1286I GDGCHC 1 cut(s) 1048
Bsp143I GATC 4 cut(s) 73, 133, 235, 884
BspACI CCGC 3 cut(s) 307, 310, 683
BspANI GGCC 3 cut(s) 807, 821, 871
BspCNI CTCAG 2 cut(s) 25, 829
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 4 cut(s) 39, 135, 1280, 1292
BspMAI CTGCAG 1 cut(s) 510
BspPI GGATC 3 cut(s) 68, 128, 141
BspT107I GGYRCC 2 cut(s) 37, 1290
BspTI CTTAAG 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 1 cut(s) 115
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 150, 1104
BssMI GATC 4 cut(s) 73, 133, 235, 884
BssNI GRCGYC 2 cut(s) 38, 1291
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 2 cut(s) 150, 1104
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 2 cut(s) 467, 1149
Bst4CI ACNGT 4 cut(s) 246, 991, 1043, 1176
BstACI GRCGYC 2 cut(s) 38, 1291
BstAFI CTTAAG 1 cut(s) 271
BstAPI GCANNNNNTGC 1 cut(s) 873
BstC8I GCNNGC 6 cut(s) 36, 314, 428, 869, 940, 1101
BstDEI CTNAG 2 cut(s) 12, 816
BstF5I GGATG 4 cut(s) 10, 76, 960, 1195
BstH2I RGCGCY 2 cut(s) 41, 1294
BstHHI GCGC 4 cut(s) 34, 40, 1165, 1293
BstKTI GATC 4 cut(s) 76, 136, 238, 887
BstMAI GTCTC 2 cut(s) 819, 1136
BstMBI GATC 4 cut(s) 73, 133, 235, 884
BstMWI GCNNNNNNNGC 7 cut(s) 548, 864, 873, 944, 1100, 1217, 1226
BstNI CCWGG 2 cut(s) 467, 1149
BstSCI CCNGG 3 cut(s) 465, 568, 1147
BstSFI CTRYAG 1 cut(s) 506
BstSLI GKGCMC 1 cut(s) 1048
BstV1I GCAGC 3 cut(s) 217, 492, 604
BstV2I GAAGAC 2 cut(s) 289, 726
BstX2I RGATCY 1 cut(s) 133
BstXI CCANNNNNNTGG 1 cut(s) 91
BstYI RGATCY 1 cut(s) 133
BsuRI GGCC 3 cut(s) 807, 821, 871
BtgZI GCGATG 3 cut(s) 794, 1209, 1215
BtsCI GGATG 4 cut(s) 10, 76, 960, 1195
BtsIMutI CAGTG 3 cut(s) 251, 987, 1048
Cac8I GCNNGC 6 cut(s) 36, 314, 428, 869, 940, 1101
CciI TCATGA 1 cut(s) 415
CfoI GCGC 4 cut(s) 34, 40, 1165, 1293
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 712, 806
Csp6I GTAC 3 cut(s) 443, 584, 1172
CviAII CATG 6 cut(s) 91, 166, 416, 514, 764, 859
CviQI GTAC 3 cut(s) 443, 584, 1172
DdeI CTNAG 2 cut(s) 12, 816
DinI GGCGCC 2 cut(s) 39, 1292
DpnI GATC 4 cut(s) 75, 135, 237, 886
DpnII GATC 4 cut(s) 73, 133, 235, 884
DraIII CACNNNGTG 1 cut(s) 94
Eco130I CCWWGG 2 cut(s) 150, 1104
Eco147I AGGCCT 2 cut(s) 821, 871
Eco47I GGWCC 1 cut(s) 712
Eco57I CTGAAG 3 cut(s) 314, 339, 996
EcoRI GAATTC 2 cut(s) 266, 904
EcoRII CCWGG 2 cut(s) 465, 1147
EcoT14I CCWWGG 2 cut(s) 150, 1104
EgeI GGCGCC 2 cut(s) 39, 1292
EheI GGCGCC 2 cut(s) 39, 1292
ErhI CCWWGG 2 cut(s) 150, 1104
FaeI CATG 6 cut(s) 94, 169, 419, 517, 767, 862
FatI CATG 6 cut(s) 90, 165, 415, 513, 763, 858
FauNDI CATATG 2 cut(s) 58, 217
Fnu4HI GCNGC 4 cut(s) 206, 310, 506, 593
FokI GGATG 4 cut(s) 17, 83, 947, 1202
Fsp4HI GCNGC 4 cut(s) 206, 310, 506, 593
FspBI CTAG 3 cut(s) 117, 557, 882
GlaI GCGC 4 cut(s) 33, 39, 1164, 1292
GluI GCNGC 4 cut(s) 206, 310, 506, 593
GsuI CTGGAG 1 cut(s) 1284
HaeII RGCGCY 2 cut(s) 41, 1294
HaeIII GGCC 3 cut(s) 807, 821, 871
HapII CCGG 3 cut(s) 35, 569, 1166
HhaI GCGC 4 cut(s) 34, 40, 1165, 1293
Hin1I GRCGYC 2 cut(s) 38, 1291
Hin1II CATG 6 cut(s) 94, 169, 419, 517, 767, 862
Hin6I GCGC 4 cut(s) 32, 38, 1163, 1291
HinP1I GCGC 4 cut(s) 32, 38, 1163, 1291
HindIII AAGCTT 2 cut(s) 424, 1241
HinfI GANTC 7 cut(s) 340, 412, 520, 679, 731, 1058, 1144
HpaII CCGG 3 cut(s) 35, 569, 1166
HphI GGTGA 3 cut(s) 296, 870, 891
Hpy188I TCNGA 3 cut(s) 601, 1180, 1236
Hpy188III TCNNGA 7 cut(s) 199, 239, 344, 416, 647, 1014, 1055
HpyAV CCTTC 2 cut(s) 87, 934
HpyCH4III ACNGT 4 cut(s) 246, 991, 1043, 1176
HpyCH4V TGCA 6 cut(s) 257, 337, 508, 562, 592, 947
HpyF10VI GCNNNNNNNGC 7 cut(s) 548, 864, 873, 944, 1100, 1217, 1226
HpyF3I CTNAG 2 cut(s) 12, 816
Hsp92I GRCGYC 2 cut(s) 38, 1291
Hsp92II CATG 6 cut(s) 94, 169, 419, 517, 767, 862
HspAI GCGC 4 cut(s) 32, 38, 1163, 1291
KasI GGCGCC 2 cut(s) 37, 1290
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Kzo9I GATC 4 cut(s) 73, 133, 235, 884
Lsp1109I GCAGC 3 cut(s) 217, 492, 604
LweI GCATC 4 cut(s) 460, 771, 1150, 1207
MaeI CTAG 3 cut(s) 117, 557, 882
MaeIII GTNAC 1 cut(s) 835
MalI GATC 4 cut(s) 75, 135, 237, 886
MboI GATC 4 cut(s) 73, 133, 235, 884
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 1048
MluCI AATT 7 cut(s) 184, 266, 318, 476, 791, 904, 1017
Mly113I GGCGCC 2 cut(s) 38, 1291
MlyI GAGTC 2 cut(s) 740, 1138
MmeI TCCRAC 3 cut(s) 108, 417, 690
MreI CGCCGGCG 1 cut(s) 34
MroNI GCCGGC 1 cut(s) 34
MroXI GAANNNNTTC 2 cut(s) 159, 756
MseI TTAA 3 cut(s) 210, 272, 794
MslI CAYNNNNRTG 2 cut(s) 857, 979
MspA1I CMGCKG 1 cut(s) 505
MspCI CTTAAG 1 cut(s) 271
MspI CCGG 3 cut(s) 35, 569, 1166
MspR9I CCNGG 3 cut(s) 467, 570, 1149
Mva1269I GAATGC 1 cut(s) 919
MvaI CCWGG 2 cut(s) 467, 1149
MwoI GCNNNNNNNGC 7 cut(s) 548, 864, 873, 944, 1100, 1217, 1226
NaeI GCCGGC 1 cut(s) 36
NarI GGCGCC 2 cut(s) 38, 1291
NciI CCSGG 1 cut(s) 570
NdeI CATATG 2 cut(s) 58, 217
NdeII GATC 4 cut(s) 73, 133, 235, 884
NgoMIV GCCGGC 1 cut(s) 34
NlaIII CATG 6 cut(s) 94, 169, 419, 517, 767, 862
NlaIV GGNNCC 4 cut(s) 39, 135, 1280, 1292
NmeAIII GCCGAG 1 cut(s) 1120
PagI TCATGA 1 cut(s) 415
PceI AGGCCT 2 cut(s) 821, 871
PctI GAATGC 1 cut(s) 919
PdiI GCCGGC 1 cut(s) 36
PdmI GAANNNNTTC 2 cut(s) 159, 756
PfeI GAWTC 5 cut(s) 340, 412, 520, 679, 1058
PkrI GCNGC 4 cut(s) 207, 311, 507, 594
PleI GAGTC 2 cut(s) 739, 1138
PluTI GGCGCC 2 cut(s) 41, 1294
PpsI GAGTC 2 cut(s) 739, 1138
Psp6I CCWGG 2 cut(s) 465, 1147
PspGI CCWGG 2 cut(s) 465, 1147
PspN4I GGNNCC 4 cut(s) 39, 135, 1280, 1292
PspPI GGNCC 2 cut(s) 712, 806
PstI CTGCAG 1 cut(s) 510
PsuI RGATCY 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 505
RsaI GTAC 3 cut(s) 444, 585, 1173
RsaNI GTAC 3 cut(s) 443, 584, 1172
RseI CAYNNNNRTG 2 cut(s) 857, 979
SaqAI TTAA 3 cut(s) 210, 272, 794
SatI GCNGC 4 cut(s) 206, 310, 506, 593
Sau3AI GATC 4 cut(s) 73, 133, 235, 884
Sau96I GGNCC 2 cut(s) 712, 806
SchI GAGTC 2 cut(s) 740, 1138
ScrFI CCNGG 3 cut(s) 467, 570, 1149
SduI GDGCHC 1 cut(s) 1048
SfaNI GCATC 4 cut(s) 460, 771, 1150, 1207
SfcI CTRYAG 1 cut(s) 506
SfoI GGCGCC 2 cut(s) 39, 1292
SgrAI CRCCGGYG 1 cut(s) 34
SinI GGWCC 1 cut(s) 712
SmiMI CAYNNNNRTG 2 cut(s) 857, 979
SmlI CTYRAG 3 cut(s) 271, 431, 719
SmoI CTYRAG 3 cut(s) 271, 431, 719
SpeI ACTAGT 1 cut(s) 556
Sse9I AATT 7 cut(s) 184, 266, 318, 476, 791, 904, 1017
SseBI AGGCCT 2 cut(s) 821, 871
SsiI CCGC 3 cut(s) 307, 310, 683
SspDI GGCGCC 2 cut(s) 37, 1290
SspI AATATT 1 cut(s) 301
SspMI CTAG 3 cut(s) 117, 557, 882
StuI AGGCCT 2 cut(s) 821, 871
StyD4I CCNGG 3 cut(s) 465, 568, 1147
StyI CCWWGG 2 cut(s) 150, 1104
TaaI ACNGT 4 cut(s) 246, 991, 1043, 1176
TaqI TCGA 4 cut(s) 191, 238, 635, 1064
TasI AATT 7 cut(s) 184, 266, 318, 476, 791, 904, 1017
TatI WGTACW 2 cut(s) 583, 1171
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 5 cut(s) 340, 412, 520, 679, 1058
Tru1I TTAA 3 cut(s) 210, 272, 794
Tru9I TTAA 3 cut(s) 210, 272, 794
TscAI CASTG 3 cut(s) 251, 994, 1048
TseI GCWGC 3 cut(s) 205, 505, 592
TspDTI ATGAA 4 cut(s) 311, 875, 926, 1040
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 3 cut(s) 251, 994, 1048
Vha464I CTTAAG 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 712
XapI RAATTY 3 cut(s) 266, 476, 904
XmnI GAANNNNTTC 2 cut(s) 159, 756
XspI CTAG 3 cut(s) 117, 557, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.