pycom10g02900

FAD binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002765
Physical Location & Seq
Reverse (-)
3228260 .. 3229376
1117 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 879 bp
ATGAAATACTGTCATGTCAATAGAGGTTGGGAAGCACGATGCTTGAAACGGAGTGATCTGATCGCAACACTAGCAGAGAGCTTGCCAGTTGGTACCATACGCCTTGGATGCCAAGCAATATCGGTAAAGTTGGACTCCTTAACTTCCCATCCAACTCTTCAACTCCACAACGGAAGCACCATCAAAGCCAAGGTTTTGATCGGATGTGATGGAACAAAATCAGTTGTCGCAGATTTCGTCGGTGTAAAACCATCAAGGCCGTTCATGTTAGCGGGGGTTAGAGGTTTTACAGTTTACCCAGGTGGTCATAATTTTGGAAACGAGTTAGTAATGGTTAAGGGTGACAAGAAGACAATTGGAAGAATTCCAGTTCATGATAACTTGGTCTCCTGGTTCGTCACTCACCAAGTTCATGGCAGACAAGATCCATCGGAGGTTTCGAAGGATCCAGAGCTCATTCGACAACTGACATTACAGTTAATCGAAGAATTTCCTTCAGAAATGGTAGACATGATAAGAAAGAGTGACCTAGAATCAGTATCTCACGTGCTCTTGAGATATCGTCCGCCATGGGAGATACTGGTAGAAAAATTTCGAAGGGGAAGCGTGACGGTGGCTGGAGATGCCATGCATGTAATGGGGCCCTTCCTCGGGCAGGGAGGCTCCGCAGGCATCGAAGACGCCATTGTCATTGCTAGAAGCTTGGCTCCGACATTGGCCAAAAATTACGATAGAAGATCGAGCGGAAGGAATGGAATGATGGTCGAAGTGGGAGAGGCTCTCGACAAGTATGTGAAAGAGAGAAGGATGAGACTGGTGATGCTCTCTGCACAAACTTATCTACTTGGTTTGCTGCAACAAAATCCAGGGCCGATGTAA

Protein Analysis

293

Amino Acids

32.27

Weight (kDa)

9.37

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 25 - 239 5.1e-15 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 686
Acc65I GGTACC 1 cut(s) 92
AccB1I GGYRCC 1 cut(s) 92
AccBSI CCGCTC 1 cut(s) 744
AccI GTMKAC 1 cut(s) 507
AciI CCGC 4 cut(s) 272, 566, 666, 744
AclWI GGATC 3 cut(s) 419, 440, 453
AcoI YGGCCR 1 cut(s) 717
AcsI RAATTY 3 cut(s) 363, 488, 590
AcuI CTGAAG 1 cut(s) 480
AcvI CACGTG 1 cut(s) 547
AcyI GRCGYC 1 cut(s) 681
AfaI GTAC 1 cut(s) 94
AfiI CCNNNNNNNGG 3 cut(s) 650, 651, 655
AgsI TTSAA 2 cut(s) 46, 161
AjnI CCWGG 3 cut(s) 298, 389, 865
AluBI AGCT 3 cut(s) 81, 454, 702
AluI AGCT 3 cut(s) 81, 454, 702
Alw21I GWGCWC 2 cut(s) 456, 552
Alw26I GTCTC 2 cut(s) 391, 805
AlwI GGATC 3 cut(s) 419, 440, 453
Ama87I CYCGRG 1 cut(s) 650
AoxI GGCC 4 cut(s) 257, 641, 717, 869
ApaI GGGCCC 1 cut(s) 645
ApeKI GCWGC 1 cut(s) 853
ApoI RAATTY 3 cut(s) 363, 488, 590
Asp700I GAANNNNTTC 2 cut(s) 489, 591
Asp718I GGTACC 1 cut(s) 92
AspS9I GGNCC 3 cut(s) 641, 642, 869
AsuHPI GGTGA 3 cut(s) 353, 395, 829
AsuII TTCGAA 2 cut(s) 440, 595
AvaI CYCGRG 1 cut(s) 650
BaeGI GKGCMC 1 cut(s) 645
BalI TGGCCA 1 cut(s) 719
BamHI GGATCC 1 cut(s) 445
BanI GGYRCC 1 cut(s) 92
BanII GRGCYC 2 cut(s) 456, 645
BbrPI CACGTG 1 cut(s) 547
BbsI GAAGAC 2 cut(s) 356, 684
Bbv12I GWGCWC 2 cut(s) 456, 552
BbvI GCAGC 1 cut(s) 840
BccI CCATC 6 cut(s) 156, 188, 203, 259, 436, 754
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 3 cut(s) 300, 391, 867
BcoDI GTCTC 2 cut(s) 391, 805
BfaI CTAG 3 cut(s) 71, 530, 696
BisI GCNGC 1 cut(s) 854
BlsI GCNGC 1 cut(s) 855
Bme1390I CCNGG 3 cut(s) 300, 391, 867
BmeT110I CYCGRG 1 cut(s) 650
BmgT120I GGNCC 3 cut(s) 641, 642, 869
BmiI GGNNCC 6 cut(s) 94, 447, 642, 643, 664, 708
BmrFI CCNGG 3 cut(s) 300, 391, 867
BmsI GCATC 5 cut(s) 29, 98, 613, 681, 810
BpiI GAAGAC 2 cut(s) 356, 684
BplI GAGNNNNNCTC 2 cut(s) 765, 797
BpmI CTGGAG 1 cut(s) 639
Bpu14I TTCGAA 2 cut(s) 440, 595
BpuEI CTTGAG 1 cut(s) 574
BsaAI YACGTR 1 cut(s) 547
BsaHI GRCGYC 1 cut(s) 681
BsaI GGTCTC 1 cut(s) 391
BsaJI CCNNGG 6 cut(s) 103, 189, 298, 569, 649, 866
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 3 cut(s) 650, 651, 655
Bse1I ACTGG 4 cut(s) 86, 368, 585, 819
Bse3DI GCAATG 1 cut(s) 690
BseBI CCWGG 3 cut(s) 300, 391, 867
BseDI CCNNGG 6 cut(s) 103, 189, 298, 569, 649, 866
BseGI GGATG 4 cut(s) 113, 148, 209, 813
BseLI CCNNNNNNNGG 3 cut(s) 650, 651, 655
BseMI GCAATG 1 cut(s) 690
BseNI ACTGG 4 cut(s) 86, 368, 585, 819
BseSI GKGCMC 1 cut(s) 645
BseXI GCAGC 1 cut(s) 840
BsgI GTGCAG 1 cut(s) 813
BshFI GGCC 4 cut(s) 259, 643, 719, 871
BshNI GGYRCC 1 cut(s) 92
BsiHKAI GWGCWC 2 cut(s) 456, 552
BsiHKCI CYCGRG 1 cut(s) 650
BslI CCNNNNNNNGG 3 cut(s) 650, 651, 655
BsmAI GTCTC 2 cut(s) 391, 805
BsnI GGCC 4 cut(s) 259, 643, 719, 871
Bso31I GGTCTC 1 cut(s) 391
BsoBI CYCGRG 1 cut(s) 650
Bsp119I TTCGAA 2 cut(s) 440, 595
Bsp120I GGGCCC 1 cut(s) 641
Bsp1286I GDGCHC 3 cut(s) 456, 552, 645
Bsp143I GATC 6 cut(s) 55, 60, 198, 424, 445, 737
Bsp19I CCATGG 1 cut(s) 569
BspACI CCGC 4 cut(s) 272, 566, 666, 744
BspANI GGCC 4 cut(s) 259, 643, 719, 871
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 6 cut(s) 94, 447, 642, 643, 664, 708
BspPI GGATC 3 cut(s) 419, 440, 453
BspT104I TTCGAA 2 cut(s) 440, 595
BspT107I GGYRCC 1 cut(s) 92
BspTNI GGTCTC 1 cut(s) 391
BsrBI CCGCTC 1 cut(s) 744
BsrDI GCAATG 1 cut(s) 690
BsrI ACTGG 4 cut(s) 86, 368, 585, 819
BssECI CCNNGG 6 cut(s) 103, 189, 298, 569, 649, 866
BssMI GATC 6 cut(s) 55, 60, 198, 424, 445, 737
BssNI GRCGYC 1 cut(s) 681
BssT1I CCWWGG 3 cut(s) 103, 189, 569
Bst2UI CCWGG 3 cut(s) 300, 391, 867
Bst4CI ACNGT 4 cut(s) 11, 292, 477, 613
Bst6I CTCTTC 1 cut(s) 162
BstACI GRCGYC 1 cut(s) 681
BstBAI YACGTR 1 cut(s) 547
BstBI TTCGAA 2 cut(s) 440, 595
BstC8I GCNNGC 2 cut(s) 83, 670
BstDSI CCRYGG 1 cut(s) 569
BstENI CCTNNNNNAGG 1 cut(s) 653
BstF5I GGATG 4 cut(s) 113, 148, 209, 813
BstKTI GATC 6 cut(s) 58, 63, 201, 427, 448, 740
BstMAI GTCTC 2 cut(s) 391, 805
BstMBI GATC 6 cut(s) 55, 60, 198, 424, 445, 737
BstMWI GCNNNNNNNGC 4 cut(s) 71, 108, 623, 669
BstNI CCWGG 3 cut(s) 300, 391, 867
BstNSI RCATGY 1 cut(s) 635
BstSCI CCNGG 3 cut(s) 298, 389, 865
BstSLI GKGCMC 1 cut(s) 645
BstV1I GCAGC 1 cut(s) 840
BstV2I GAAGAC 2 cut(s) 356, 684
BstX2I RGATCY 2 cut(s) 424, 445
BstXI CCANNNNNNTGG 1 cut(s) 413
BstYI RGATCY 2 cut(s) 424, 445
BsuRI GGCC 4 cut(s) 259, 643, 719, 871
BtgI CCRYGG 1 cut(s) 569
BtsCI GGATG 4 cut(s) 113, 148, 209, 813
Cac8I GCNNGC 2 cut(s) 83, 670
CciI TCATGA 1 cut(s) 373
Cfr13I GGNCC 3 cut(s) 641, 642, 869
CseI GACGC 1 cut(s) 689
Csp6I GTAC 1 cut(s) 93
CviAII CATG 8 cut(s) 14, 265, 374, 413, 511, 570, 628, 632
CviQI GTAC 1 cut(s) 93
DpnI GATC 6 cut(s) 57, 62, 200, 426, 447, 739
DpnII GATC 6 cut(s) 55, 60, 198, 424, 445, 737
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
EaeI YGGCCR 1 cut(s) 717
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
EciI GGCGGA 1 cut(s) 555
Ecl136II GAGCTC 1 cut(s) 454
Eco130I CCWWGG 3 cut(s) 103, 189, 569
Eco24I GRGCYC 2 cut(s) 456, 645
Eco31I GGTCTC 1 cut(s) 391
Eco32I GATATC 1 cut(s) 560
Eco53kI GAGCTC 1 cut(s) 454
Eco57I CTGAAG 1 cut(s) 480
Eco72I CACGTG 1 cut(s) 547
Eco88I CYCGRG 1 cut(s) 650
EcoICRI GAGCTC 1 cut(s) 454
EcoNI CCTNNNNNAGG 1 cut(s) 653
EcoO109I RGGNCCY 2 cut(s) 641, 642
EcoRI GAATTC 1 cut(s) 363
EcoRII CCWGG 3 cut(s) 298, 389, 865
EcoRV GATATC 1 cut(s) 560
EcoT14I CCWWGG 3 cut(s) 103, 189, 569
EcoT22I ATGCAT 1 cut(s) 633
EcoT38I GRGCYC 2 cut(s) 456, 645
ErhI CCWWGG 3 cut(s) 103, 189, 569
FaeI CATG 8 cut(s) 17, 268, 377, 416, 514, 573, 631, 635
FatI CATG 8 cut(s) 13, 264, 373, 412, 510, 569, 627, 631
FauI CCCGC 1 cut(s) 265
FblI GTMKAC 1 cut(s) 507
Fnu4HI GCNGC 1 cut(s) 854
FokI GGATG 4 cut(s) 120, 135, 216, 820
FriOI GRGCYC 2 cut(s) 456, 645
Fsp4HI GCNGC 1 cut(s) 854
FspBI CTAG 3 cut(s) 71, 530, 696
GluI GCNGC 1 cut(s) 854
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 4 cut(s) 259, 643, 719, 871
HgaI GACGC 1 cut(s) 689
Hin1I GRCGYC 1 cut(s) 681
Hin1II CATG 8 cut(s) 17, 268, 377, 416, 514, 573, 631, 635
HindIII AAGCTT 1 cut(s) 700
HinfI GANTC 2 cut(s) 134, 533
HphI GGTGA 3 cut(s) 353, 395, 829
Hpy166II GTNNAC 2 cut(s) 295, 508
Hpy188I TCNGA 5 cut(s) 60, 203, 433, 499, 711
Hpy188III TCNNGA 4 cut(s) 374, 449, 553, 782
Hpy8I GTNNAC 2 cut(s) 295, 508
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 6 cut(s) 436, 504, 591, 655, 741, 798
HpyCH4III ACNGT 4 cut(s) 11, 292, 477, 613
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 3 cut(s) 631, 830, 856
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 108, 623, 669
HpySE526I ACGT 1 cut(s) 546
Hsp92I GRCGYC 1 cut(s) 681
Hsp92II CATG 8 cut(s) 17, 268, 377, 416, 514, 573, 631, 635
KpnI GGTACC 1 cut(s) 96
Kzo9I GATC 6 cut(s) 55, 60, 198, 424, 445, 737
LmnI GCTCC 2 cut(s) 668, 712
Lsp1109I GCAGC 1 cut(s) 840
LweI GCATC 5 cut(s) 29, 98, 613, 681, 810
MaeI CTAG 3 cut(s) 71, 530, 696
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 4 cut(s) 341, 397, 524, 607
MalI GATC 6 cut(s) 57, 62, 200, 426, 447, 739
MbiI CCGCTC 1 cut(s) 744
MboI GATC 6 cut(s) 55, 60, 198, 424, 445, 737
MboII GAAGA 6 cut(s) 149, 361, 372, 497, 689, 747
MfeI CAATTG 1 cut(s) 354
MflI RGATCY 2 cut(s) 424, 445
MhlI GDGCHC 3 cut(s) 456, 552, 645
MlsI TGGCCA 1 cut(s) 719
MluCI AATT 6 cut(s) 310, 354, 363, 488, 590, 724
MluNI TGGCCA 1 cut(s) 719
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 3 cut(s) 111, 176, 734
MnlI CCTC 6 cut(s) 17, 275, 427, 653, 659, 769
Mox20I TGGCCA 1 cut(s) 719
Mph1103I ATGCAT 1 cut(s) 633
MroXI GAANNNNTTC 2 cut(s) 489, 591
MscI TGGCCA 1 cut(s) 719
MseI TTAA 3 cut(s) 140, 336, 479
Msp20I TGGCCA 1 cut(s) 719
MspR9I CCNGG 3 cut(s) 300, 391, 867
MunI CAATTG 1 cut(s) 354
MvaI CCWGG 3 cut(s) 300, 391, 867
MwoI GCNNNNNNNGC 4 cut(s) 71, 108, 623, 669
NcoI CCATGG 1 cut(s) 569
NdeII GATC 6 cut(s) 55, 60, 198, 424, 445, 737
NlaIII CATG 8 cut(s) 17, 268, 377, 416, 514, 573, 631, 635
NlaIV GGNNCC 6 cut(s) 94, 447, 642, 643, 664, 708
NmuCI GTSAC 4 cut(s) 341, 397, 524, 607
NsiI ATGCAT 1 cut(s) 633
NspI RCATGY 1 cut(s) 635
NspV TTCGAA 2 cut(s) 440, 595
PagI TCATGA 1 cut(s) 373
PcsI WCGNNNNNNNCGW 2 cut(s) 234, 437
PdmI GAANNNNTTC 2 cut(s) 489, 591
PfeI GAWTC 1 cut(s) 533
PkrI GCNGC 1 cut(s) 855
PleI GAGTC 1 cut(s) 128
PmaCI CACGTG 1 cut(s) 547
PmlI CACGTG 1 cut(s) 547
PpsI GAGTC 1 cut(s) 128
Ppu21I YACGTR 1 cut(s) 547
Psp124BI GAGCTC 1 cut(s) 456
Psp6I CCWGG 3 cut(s) 298, 389, 865
PspCI CACGTG 1 cut(s) 547
PspGI CCWGG 3 cut(s) 298, 389, 865
PspN4I GGNNCC 6 cut(s) 94, 447, 642, 643, 664, 708
PspOMI GGGCCC 1 cut(s) 641
PspPI GGNCC 3 cut(s) 641, 642, 869
PsuI RGATCY 2 cut(s) 424, 445
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
SacI GAGCTC 1 cut(s) 456
SaqAI TTAA 3 cut(s) 140, 336, 479
SatI GCNGC 1 cut(s) 854
Sau3AI GATC 6 cut(s) 55, 60, 198, 424, 445, 737
Sau96I GGNCC 3 cut(s) 641, 642, 869
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 3 cut(s) 300, 391, 867
SduI GDGCHC 3 cut(s) 456, 552, 645
SfaNI GCATC 5 cut(s) 29, 98, 613, 681, 810
SfuI TTCGAA 2 cut(s) 440, 595
SmlI CTYRAG 1 cut(s) 553
SmoI CTYRAG 1 cut(s) 553
Sse9I AATT 6 cut(s) 310, 354, 363, 488, 590, 724
SsiI CCGC 4 cut(s) 272, 566, 666, 744
SspMI CTAG 3 cut(s) 71, 530, 696
SstI GAGCTC 1 cut(s) 456
StyD4I CCNGG 3 cut(s) 298, 389, 865
StyI CCWWGG 3 cut(s) 103, 189, 569
TaaI ACNGT 4 cut(s) 11, 292, 477, 613
TaiI ACGT 1 cut(s) 549
TaqI TCGA 8 cut(s) 440, 460, 483, 595, 675, 740, 765, 783
TasI AATT 6 cut(s) 310, 354, 363, 488, 590, 724
TfiI GAWTC 1 cut(s) 533
Tru1I TTAA 3 cut(s) 140, 336, 479
Tru9I TTAA 3 cut(s) 140, 336, 479
TseFI GTSAC 4 cut(s) 341, 397, 524, 607
TseI GCWGC 1 cut(s) 853
Tsp45I GTSAC 4 cut(s) 341, 397, 524, 607
TspDTI ATGAA 4 cut(s) 17, 253, 362, 401
TspGWI ACGGA 2 cut(s) 64, 186
XagI CCTNNNNNAGG 1 cut(s) 653
XapI RAATTY 3 cut(s) 363, 488, 590
XceI RCATGY 1 cut(s) 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 634
XmiI GTMKAC 1 cut(s) 507
XmnI GAANNNNTTC 2 cut(s) 489, 591
XspI CTAG 3 cut(s) 71, 530, 696
Zsp2I ATGCAT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.