pycom111g01350

Lysine histidine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0003493
Physical Location & Seq
Reverse (-)
854999 .. 856564
1566 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01350.2

Sequence Viewer

Length: 903 bp
ATGGTTGGAGCGGGTGTTCTCAGTCTCCCTTACGCCATGTCCAATCTTGGATGGGGTCCTGGTGTGGTAATTCTAGTGCTGTCATGGATCATCACACTGTACACACTGTGGCAAATGGTTGAAATGCATGAAATGGTTCCAGGCAAACGGTTTGATCGATACCACGAGCTCGGGCAGTATGCATTTGGTGAAAAGCTTGGCCTTTACATTGTTGTGCCTCAACAGCTCATCGTCGAGGTCGGTGTCAACATTGTATACATGGTTACCGGAGGAAAATCACTGCAGAAGGTCCACAATTCTGTGTGCTCAGACTGTAAAAATATCAAGTTAACTTACTTCATAATGATCGATGCATCTGTTCACTTTGTCCTCTCCCACCTTCCCAACTTCAACTCCATCTCTGGTGTGTCTTTGGCTGCTGCAGTCATGTCCTTGAGTTACTCTACCATCGCTTGGGCAGCCTCTGTGGACAAAGGTGTGGTAGAAAATGTCTCGTATGATTACAGATCTAAGACTACAACAGGCAATGTGTTCAACTTCTTCAATGCCTTGGGTGACGTGGCTTTTGCCTATGCTGGTCACAATGTTGTTCTTGAAATCCAAGCAACAATTCCATCTACACCTGAGAAGCCATCCAAGGGACCCATGTGGAGAGGAGTCATCGTCGCCTACATCGTAGTCGCCTTGTGCTACTTCCCAGTTGCTCTGATCGGATACTACATTTTTGGCAAATCAGTCGAAGATAACATCCTCATGTCACTCGAAAAACCTAACTGGCTCATTGCAATGGCCAACATGTTTGTTGTCATCCATGTCATCGGAAGCTATCAGATTTACGCAATGCCGGTGTTTGAGATCGATCGAAACTGTACTGGTAAAGAAGTTGAATTTCGAACCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

301

Amino Acids

33.31

Weight (kDa)

6.37

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 453
AccBSI CCGCTC 1 cut(s) 11
AccI GTMKAC 1 cut(s) 255
AciI CCGC 1 cut(s) 11
AclWI GGATC 1 cut(s) 95
AcoI YGGCCR 1 cut(s) 789
AcsI RAATTY 1 cut(s) 887
AdeI CACNNNGTG 1 cut(s) 108
AfaI GTAC 2 cut(s) 101, 871
AfiI CCNNNNNNNGG 2 cut(s) 453, 638
AflIII ACRYGT 1 cut(s) 795
AgsI TTSAA 6 cut(s) 122, 391, 535, 544, 596, 887
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 2 cut(s) 58, 139
AjuI GAANNNNNNNTTGG 2 cut(s) 594, 626
AloI GAACNNNNNNTCC 1 cut(s) 32
AluBI AGCT 4 cut(s) 169, 196, 226, 825
AluI AGCT 4 cut(s) 169, 196, 226, 825
Alw21I GWGCWC 2 cut(s) 171, 308
Alw26I GTCTC 2 cut(s) 29, 496
AlwI GGATC 1 cut(s) 95
AlwNI CAGNNNCTG 1 cut(s) 464
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 2 cut(s) 199, 789
ApeKI GCWGC 3 cut(s) 416, 419, 458
ApoI RAATTY 1 cut(s) 887
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
Asp700I GAANNNNTTC 1 cut(s) 135
AspS9I GGNCC 3 cut(s) 56, 289, 641
AsuHPI GGTGA 2 cut(s) 200, 566
AsuII TTCGAA 1 cut(s) 892
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 3 cut(s) 56, 289, 641
BalI TGGCCA 1 cut(s) 791
BanII GRGCYC 1 cut(s) 171
BauI CACGAG 1 cut(s) 164
Bbv12I GWGCWC 2 cut(s) 171, 308
BbvI GCAGC 3 cut(s) 403, 406, 470
BccI CCATC 5 cut(s) 45, 404, 455, 622, 640
BcgI CGANNNNNNTGC 2 cut(s) 718, 752
BciT130I CCWGG 2 cut(s) 60, 141
BciVI GTATCC 1 cut(s) 707
BcoDI GTCTC 2 cut(s) 29, 496
BfaI CTAG 2 cut(s) 74, 901
BfmI CTRYAG 2 cut(s) 281, 420
BfuI GTATCC 1 cut(s) 707
BglII AGATCT 1 cut(s) 506
BisI GCNGC 3 cut(s) 417, 420, 459
BlsI GCNGC 3 cut(s) 418, 421, 460
Bme1390I CCNGG 2 cut(s) 60, 141
Bme18I GGWCC 3 cut(s) 56, 289, 641
BmeT110I CYCGRG 1 cut(s) 170
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 3 cut(s) 56, 289, 641
BmiI GGNNCC 4 cut(s) 57, 138, 642, 643
BmrFI CCNGG 2 cut(s) 60, 141
BmrI ACTGGG 1 cut(s) 692
BmsI GCATC 2 cut(s) 340, 362
BmuI ACTGGG 1 cut(s) 692
Bpu14I TTCGAA 1 cut(s) 892
BpuEI CTTGAG 1 cut(s) 454
Bsa29I ATCGAT 3 cut(s) 157, 348, 858
BsaJI CCNNGG 2 cut(s) 549, 636
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 5 cut(s) 30, 377, 407, 648, 678
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 638
Bse118I RCCGGY 1 cut(s) 844
Bse1I ACTGG 3 cut(s) 698, 779, 877
Bse3DI GCAATG 4 cut(s) 532, 780, 792, 846
BseBI CCWGG 2 cut(s) 60, 141
BseCI ATCGAT 3 cut(s) 157, 348, 858
BseDI CCNNGG 2 cut(s) 549, 636
BseGI GGATG 4 cut(s) 56, 632, 747, 807
BseLI CCNNNNNNNGG 2 cut(s) 453, 638
BseMI GCAATG 4 cut(s) 532, 780, 792, 846
BseMII CTCAG 3 cut(s) 34, 321, 615
BseNI ACTGG 3 cut(s) 698, 779, 877
BseRI GAGGAG 1 cut(s) 669
BseXI GCAGC 3 cut(s) 403, 406, 470
Bsh1285I CGRYCG 1 cut(s) 862
BshFI GGCC 2 cut(s) 201, 791
BshVI ATCGAT 3 cut(s) 157, 348, 858
BsiEI CGRYCG 1 cut(s) 862
BsiHKAI GWGCWC 2 cut(s) 171, 308
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 2 cut(s) 267, 845
BslFI GGGAC 1 cut(s) 654
BslI CCNNNNNNNGG 2 cut(s) 453, 638
BsmAI GTCTC 2 cut(s) 29, 496
BsmFI GGGAC 1 cut(s) 654
BsnI GGCC 2 cut(s) 201, 791
BsoBI CYCGRG 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 892
Bsp1286I GDGCHC 2 cut(s) 171, 308
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 7 cut(s) 87, 154, 345, 506, 708, 855, 859
BspACI CCGC 1 cut(s) 11
BspANI GGCC 2 cut(s) 201, 791
BspCNI CTCAG 3 cut(s) 33, 320, 616
BspDI ATCGAT 3 cut(s) 157, 348, 858
BspLI GGNNCC 4 cut(s) 57, 138, 642, 643
BspMAI CTGCAG 2 cut(s) 285, 424
BspPI GGATC 1 cut(s) 95
BspT104I TTCGAA 1 cut(s) 892
BsrBI CCGCTC 1 cut(s) 11
BsrDI GCAATG 4 cut(s) 532, 780, 792, 846
BsrFI RCCGGY 1 cut(s) 844
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 3 cut(s) 698, 779, 877
BssAI RCCGGY 1 cut(s) 844
BssECI CCNNGG 2 cut(s) 549, 636
BssMI GATC 7 cut(s) 87, 154, 345, 506, 708, 855, 859
BssNAI GTATAC 1 cut(s) 256
BssSI CACGAG 1 cut(s) 164
BssT1I CCWWGG 2 cut(s) 549, 636
Bst1107I GTATAC 1 cut(s) 256
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 2 cut(s) 60, 141
Bst4CI ACNGT 5 cut(s) 99, 108, 150, 314, 869
BstAUI TGTACA 1 cut(s) 99
BstBI TTCGAA 1 cut(s) 892
BstDEI CTNAG 4 cut(s) 20, 307, 510, 624
BstEII GGTNACC 1 cut(s) 262
BstF5I GGATG 4 cut(s) 56, 632, 747, 807
BstKTI GATC 7 cut(s) 90, 157, 348, 509, 711, 858, 862
BstMAI GTCTC 2 cut(s) 29, 496
BstMBI GATC 7 cut(s) 87, 154, 345, 506, 708, 855, 859
BstMCI CGRYCG 1 cut(s) 862
BstMWI GCNNNNNNNGC 2 cut(s) 223, 458
BstNI CCWGG 2 cut(s) 60, 141
BstNSI RCATGY 1 cut(s) 799
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 2 cut(s) 58, 139
BstSFI CTRYAG 2 cut(s) 281, 420
BstV1I GCAGC 3 cut(s) 403, 406, 470
BstX2I RGATCY 1 cut(s) 506
BstYI RGATCY 1 cut(s) 506
BstZ17I GTATAC 1 cut(s) 256
Bsu15I ATCGAT 3 cut(s) 157, 348, 858
BsuI GTATCC 1 cut(s) 707
BsuRI GGCC 2 cut(s) 201, 791
BsuTUI ATCGAT 3 cut(s) 157, 348, 858
BtgZI GCGATG 1 cut(s) 433
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 4 cut(s) 56, 632, 747, 807
BtsI GCAGTG 1 cut(s) 278
BtsIMutI CAGTG 3 cut(s) 95, 104, 278
CaiI CAGNNNCTG 1 cut(s) 464
Cfr10I RCCGGY 1 cut(s) 844
Cfr13I GGNCC 3 cut(s) 56, 289, 641
ClaI ATCGAT 3 cut(s) 157, 348, 858
Csp6I GTAC 2 cut(s) 100, 870
CviAII CATG 9 cut(s) 37, 84, 128, 259, 427, 646, 754, 796, 812
CviQI GTAC 2 cut(s) 100, 870
DdeI CTNAG 4 cut(s) 20, 307, 510, 624
DpnI GATC 7 cut(s) 89, 156, 347, 508, 710, 857, 861
DpnII GATC 7 cut(s) 87, 154, 345, 506, 708, 855, 859
DraIII CACNNNGTG 1 cut(s) 108
EaeI YGGCCR 1 cut(s) 789
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 2 cut(s) 549, 636
Eco24I GRGCYC 1 cut(s) 171
Eco47I GGWCC 3 cut(s) 56, 289, 641
Eco53kI GAGCTC 1 cut(s) 169
Eco88I CYCGRG 1 cut(s) 170
Eco91I GGTNACC 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 2 cut(s) 56, 641
EcoO65I GGTNACC 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 58, 139
EcoT14I CCWWGG 2 cut(s) 549, 636
EcoT22I ATGCAT 3 cut(s) 129, 184, 355
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 2 cut(s) 549, 636
FaeI CATG 9 cut(s) 40, 87, 131, 262, 430, 649, 757, 799, 815
FaqI GGGAC 1 cut(s) 654
FatI CATG 9 cut(s) 36, 83, 127, 258, 426, 645, 753, 795, 811
FauI CCCGC 1 cut(s) 4
FblI GTMKAC 1 cut(s) 255
Fnu4HI GCNGC 3 cut(s) 417, 420, 459
FokI GGATG 4 cut(s) 63, 619, 734, 794
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 3 cut(s) 417, 420, 459
FspBI CTAG 2 cut(s) 74, 901
GluI GCNGC 3 cut(s) 417, 420, 459
HaeIII GGCC 2 cut(s) 201, 791
HapII CCGG 2 cut(s) 267, 845
Hin1II CATG 9 cut(s) 40, 87, 131, 262, 430, 649, 757, 799, 815
HincII GTYRAC 2 cut(s) 247, 330
HindII GTYRAC 2 cut(s) 247, 330
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 1 cut(s) 657
HpaI GTTAAC 1 cut(s) 330
HpaII CCGG 2 cut(s) 267, 845
HphI GGTGA 2 cut(s) 200, 566
Hpy166II GTNNAC 7 cut(s) 102, 247, 256, 292, 330, 361, 469
Hpy188I TCNGA 5 cut(s) 310, 708, 713, 821, 831
Hpy188III TCNNGA 1 cut(s) 593
Hpy8I GTNNAC 7 cut(s) 102, 247, 256, 292, 330, 361, 469
Hpy99I CGWCG 2 cut(s) 236, 668
HpyAV CCTTC 2 cut(s) 280, 389
HpyCH4III ACNGT 5 cut(s) 99, 108, 150, 314, 869
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 6 cut(s) 127, 182, 283, 353, 422, 785
HpyF10VI GCNNNNNNNGC 2 cut(s) 223, 458
HpyF3I CTNAG 4 cut(s) 20, 307, 510, 624
HpySE526I ACGT 1 cut(s) 558
Hsp92II CATG 9 cut(s) 40, 87, 131, 262, 430, 649, 757, 799, 815
KflI GGGWCCC 1 cut(s) 641
KspAI GTTAAC 1 cut(s) 330
Kzo9I GATC 7 cut(s) 87, 154, 345, 506, 708, 855, 859
LmnI GCTCC 1 cut(s) 8
Lsp1109I GCAGC 3 cut(s) 403, 406, 470
LweI GCATC 2 cut(s) 340, 362
MaeI CTAG 2 cut(s) 74, 901
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 5 cut(s) 262, 437, 554, 578, 756
MalI GATC 7 cut(s) 89, 156, 347, 508, 710, 857, 861
MbiI CCGCTC 1 cut(s) 11
MboI GATC 7 cut(s) 87, 154, 345, 506, 708, 855, 859
MboII GAAGA 2 cut(s) 532, 752
MflI RGATCY 1 cut(s) 506
MhlI GDGCHC 2 cut(s) 171, 308
MlsI TGGCCA 1 cut(s) 791
MluCI AATT 4 cut(s) 69, 295, 609, 887
MluNI TGGCCA 1 cut(s) 791
MlyI GAGTC 1 cut(s) 666
MnlI CCTC 7 cut(s) 228, 229, 263, 380, 472, 647, 761
Mox20I TGGCCA 1 cut(s) 791
Mph1103I ATGCAT 3 cut(s) 129, 184, 355
MroXI GAANNNNTTC 1 cut(s) 135
MscI TGGCCA 1 cut(s) 791
MseI TTAA 1 cut(s) 329
MslI CAYNNNNRTG 3 cut(s) 212, 752, 785
Msp20I TGGCCA 1 cut(s) 791
MspI CCGG 2 cut(s) 267, 845
MspR9I CCNGG 2 cut(s) 60, 141
MvaI CCWGG 2 cut(s) 60, 141
MwoI GCNNNNNNNGC 2 cut(s) 223, 458
NdeII GATC 7 cut(s) 87, 154, 345, 506, 708, 855, 859
NlaIII CATG 9 cut(s) 40, 87, 131, 262, 430, 649, 757, 799, 815
NlaIV GGNNCC 4 cut(s) 57, 138, 642, 643
NmuCI GTSAC 3 cut(s) 554, 578, 756
NsiI ATGCAT 3 cut(s) 129, 184, 355
NspI RCATGY 1 cut(s) 799
NspV TTCGAA 1 cut(s) 892
PciI ACATGT 1 cut(s) 795
PcsI WCGNNNNNNNCGW 2 cut(s) 154, 672
PdmI GAANNNNTTC 1 cut(s) 135
PflMI CCANNNNNTGG 1 cut(s) 453
PkrI GCNGC 3 cut(s) 418, 421, 460
Ple19I CGATCG 1 cut(s) 862
PleI GAGTC 1 cut(s) 665
PpsI GAGTC 1 cut(s) 665
PpuMI RGGWCCY 2 cut(s) 56, 641
PscI ACATGT 1 cut(s) 795
Psp124BI GAGCTC 1 cut(s) 171
Psp5II RGGWCCY 2 cut(s) 56, 641
Psp6I CCWGG 2 cut(s) 58, 139
PspEI GGTNACC 1 cut(s) 262
PspGI CCWGG 2 cut(s) 58, 139
PspN4I GGNNCC 4 cut(s) 57, 138, 642, 643
PspPI GGNCC 3 cut(s) 56, 289, 641
PspPPI RGGWCCY 2 cut(s) 56, 641
PstI CTGCAG 2 cut(s) 285, 424
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 1 cut(s) 506
PvuI CGATCG 1 cut(s) 862
RsaI GTAC 2 cut(s) 101, 871
RsaNI GTAC 2 cut(s) 100, 870
RseI CAYNNNNRTG 3 cut(s) 212, 752, 785
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 1 cut(s) 329
SatI GCNGC 3 cut(s) 417, 420, 459
Sau3AI GATC 7 cut(s) 87, 154, 345, 506, 708, 855, 859
Sau96I GGNCC 3 cut(s) 56, 289, 641
SchI GAGTC 1 cut(s) 666
ScrFI CCNGG 2 cut(s) 60, 141
SduI GDGCHC 2 cut(s) 171, 308
SfaNI GCATC 2 cut(s) 340, 362
SfcI CTRYAG 2 cut(s) 281, 420
SfuI TTCGAA 1 cut(s) 892
SinI GGWCC 3 cut(s) 56, 289, 641
SmiMI CAYNNNNRTG 3 cut(s) 212, 752, 785
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
Sse9I AATT 4 cut(s) 69, 295, 609, 887
SsiI CCGC 1 cut(s) 11
SspMI CTAG 2 cut(s) 74, 901
SstI GAGCTC 1 cut(s) 171
StyD4I CCNGG 2 cut(s) 58, 139
StyI CCWWGG 2 cut(s) 549, 636
TaaI ACNGT 5 cut(s) 99, 108, 150, 314, 869
TaiI ACGT 1 cut(s) 561
TaqI TCGA 8 cut(s) 157, 234, 348, 738, 762, 858, 862, 892
TasI AATT 4 cut(s) 69, 295, 609, 887
TatI WGTACW 2 cut(s) 99, 869
Tru1I TTAA 1 cut(s) 329
Tru9I TTAA 1 cut(s) 329
TscAI CASTG 3 cut(s) 102, 111, 285
TseFI GTSAC 3 cut(s) 554, 578, 756
TseI GCWGC 3 cut(s) 416, 419, 458
Tsp45I GTSAC 3 cut(s) 554, 578, 756
TspDTI ATGAA 2 cut(s) 144, 328
TspRI CASTG 3 cut(s) 102, 111, 285
Van91I CCANNNNNTGG 1 cut(s) 453
VpaK11BI GGWCC 3 cut(s) 56, 289, 641
XapI RAATTY 1 cut(s) 887
XceI RCATGY 1 cut(s) 799
XmiI GTMKAC 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 135
XspI CTAG 2 cut(s) 74, 901
Zsp2I ATGCAT 3 cut(s) 129, 184, 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.