RchiOBHm_Chr2g0174521

Lysine histidine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003493
Physical Location & Seq
Forward (+)
87081540 .. 87083066
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54167

Sequence Viewer

Length: 810 bp
ATGGATGAAAAATTAGCCAAGGAAAAGGCAATTAATGAGTGGCTTCCAATTATTTCTTCGAGGAATGCAAAATGGTGGTATTCGGCTTTTCACAATGTTACGGCCGAGGTTGGAGCTGGTGTTCTCGGTCTCCCTTATGCCATGGCTGAGCTTGGATGTTTTGGTCCTGGTATAGTTATTTTGGTCCTTTCATGGGTCATCACATTGTACACACTATGGCAAATGGTTGAGATGCATGAGATGGTTCCAGGGAAACGTTTTGATCGTTACCACGAATTGGGTCAGCATGCATTTGGTGAAAAGCTTGGTCTTTACATTGTGGTGCCTCAGCAACTCATTCTCCAATGCATTTGGCCGCAGCTGTCATTAGGATATTACATATATGGAAATTCAAATGATGACAACATCCTCGTCTCATTGGAAAGACCCAAATGGCTAATTGCAATGGCCAACATGTTTGTTGTCGTCCATGTTATTGGAAGCTATCAGTGGGATTTCAGGCCAAGTTTTATGCTTCGCTTCATTTCACGAGCTTCATATGTTGCAATTACCATGTTTGTCGGAATCAAATTCCCTTTCTTCAGTGGTCTCCTTGGATTTTTCGGAGGATTTTCTTTTGCCCCAACAACATATTTTCTCCCTTGCATGATGTGGCTTGCCATCTACAAACCAAAGAGATTCGGCTTATCCTGGTGGTGCAACTATATGTATATCTGCATCTTCTTTGGTGTTCTTTTGATGGTTCTATCACCAATTGGAGGGCTGAGATCAATCATAGTTCAAGCCAAGACCTACGAGTTCTACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

269

Amino Acids

31.02

Weight (kDa)

8.71

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 22 - 116 1.4e-22 Transmembrane amino acid transporter protein
Trp_Tyr_perm PF03222 33 - 105 9.2e-08 Tryptophan/tyrosine permease family
Aa_trans PF01490 123 - 168 1.2e-06 Transmembrane amino acid transporter protein
Aa_trans PF01490 169 - 253 1.3e-17 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 322
AccB7I CCANNNNNTGG 1 cut(s) 277
AciI CCGC 1 cut(s) 356
AclI AACGTT 1 cut(s) 256
AcoI YGGCCR 3 cut(s) 102, 353, 447
AcsI RAATTY 2 cut(s) 388, 569
AcuI CTGAAG 1 cut(s) 565
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 3 cut(s) 193, 277, 758
AflIII ACRYGT 1 cut(s) 453
AgsI TTSAA 2 cut(s) 393, 782
AjnI CCWGG 3 cut(s) 166, 247, 689
AjuI GAANNNNNNNTTGG 2 cut(s) 40, 72
AloI GAACNNNNNNTCC 2 cut(s) 105, 137
AluBI AGCT 6 cut(s) 116, 151, 304, 361, 483, 533
AluI AGCT 6 cut(s) 116, 151, 304, 361, 483, 533
Alw26I GTCTC 3 cut(s) 134, 418, 593
AoxI GGCC 4 cut(s) 102, 353, 447, 500
ApeKI GCWGC 1 cut(s) 358
ApoI RAATTY 2 cut(s) 388, 569
AseI ATTAAT 1 cut(s) 33
AspS9I GGNCC 2 cut(s) 164, 184
AsuHPI GGTGA 2 cut(s) 308, 741
AvaII GGWCC 2 cut(s) 164, 184
BalI TGGCCA 1 cut(s) 449
BanI GGYRCC 1 cut(s) 322
BauI CACGAG 1 cut(s) 528
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 370
BccI CCATC 3 cut(s) 235, 668, 733
BceAI ACGGC 1 cut(s) 117
BciT130I CCWGG 3 cut(s) 168, 249, 691
BcoDI GTCTC 3 cut(s) 134, 418, 593
BisI GCNGC 2 cut(s) 356, 359
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 2 cut(s) 357, 360
Bme1390I CCNGG 3 cut(s) 168, 249, 691
Bme18I GGWCC 2 cut(s) 164, 184
BmgT120I GGNCC 2 cut(s) 164, 184
BmiI GGNNCC 2 cut(s) 246, 324
BmrFI CCNGG 3 cut(s) 168, 249, 691
BmsI GCATC 2 cut(s) 222, 726
BplI GAGNNNNNCTC 1 cut(s) 788
Bpu10I CCTNAGC 1 cut(s) 327
Bpu1102I GCTNAGC 1 cut(s) 147
BsaI GGTCTC 2 cut(s) 134, 593
BsaJI CCNNGG 5 cut(s) 18, 105, 141, 248, 592
BsaXI ACNNNNNCTCC 4 cut(s) 105, 135, 324, 354
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 277, 758
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 3 cut(s) 168, 249, 691
BseDI CCNNGG 5 cut(s) 18, 105, 141, 248, 592
BseGI GGATG 3 cut(s) 10, 161, 405
BseLI CCNNNNNNNGG 3 cut(s) 193, 277, 758
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 3 cut(s) 138, 341, 755
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 1 cut(s) 370
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 4 cut(s) 104, 355, 449, 502
BshNI GGYRCC 1 cut(s) 322
BsiEI CGRYCG 1 cut(s) 105
BslI CCNNNNNNNGG 3 cut(s) 193, 277, 758
BsmAI GTCTC 3 cut(s) 134, 418, 593
BsmBI CGTCTC 1 cut(s) 418
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 4 cut(s) 104, 355, 449, 502
Bso31I GGTCTC 2 cut(s) 134, 593
Bsp1407I TGTACA 1 cut(s) 207
Bsp143I GATC 2 cut(s) 262, 767
Bsp1720I GCTNAGC 1 cut(s) 147
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 1 cut(s) 356
BspANI GGCC 4 cut(s) 104, 355, 449, 502
BspCNI CTCAG 3 cut(s) 139, 340, 756
BspLI GGNNCC 2 cut(s) 246, 324
BspT107I GGYRCC 1 cut(s) 322
BspTNI GGTCTC 2 cut(s) 134, 593
BsrDI GCAATG 1 cut(s) 450
BsrGI TGTACA 1 cut(s) 207
BssECI CCNNGG 5 cut(s) 18, 105, 141, 248, 592
BssMI GATC 2 cut(s) 262, 767
BssSI CACGAG 1 cut(s) 528
BssT1I CCWWGG 3 cut(s) 18, 141, 592
Bst2BI CACGAG 1 cut(s) 528
Bst2UI CCWGG 3 cut(s) 168, 249, 691
BstAUI TGTACA 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 288, 657
BstDEI CTNAG 3 cut(s) 147, 327, 764
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 3 cut(s) 10, 161, 405
BstKTI GATC 2 cut(s) 265, 770
BstMAI GTCTC 3 cut(s) 134, 418, 593
BstMBI GATC 2 cut(s) 262, 767
BstMCI CGRYCG 1 cut(s) 105
BstNI CCWGG 3 cut(s) 168, 249, 691
BstNSI RCATGY 2 cut(s) 290, 457
BstSCI CCNGG 3 cut(s) 166, 247, 689
BstV1I GCAGC 1 cut(s) 370
BstXI CCANNNNNNTGG 1 cut(s) 476
BstZI CGGCCG 1 cut(s) 102
BsuRI GGCC 4 cut(s) 104, 355, 449, 502
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 3 cut(s) 10, 161, 405
BtsIMutI CAGTG 2 cut(s) 494, 589
Cac8I GCNNGC 2 cut(s) 288, 657
Cfr13I GGNCC 2 cut(s) 164, 184
Csp6I GTAC 1 cut(s) 208
CviAII CATG 8 cut(s) 142, 192, 236, 287, 454, 470, 553, 646
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 3 cut(s) 147, 327, 764
DpnI GATC 2 cut(s) 264, 769
DpnII GATC 2 cut(s) 262, 767
EaeI YGGCCR 3 cut(s) 102, 353, 447
EagI CGGCCG 1 cut(s) 102
EclXI CGGCCG 1 cut(s) 102
Eco130I CCWWGG 3 cut(s) 18, 141, 592
Eco31I GGTCTC 2 cut(s) 134, 593
Eco47I GGWCC 2 cut(s) 164, 184
Eco52I CGGCCG 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 565
EcoRII CCWGG 3 cut(s) 166, 247, 689
EcoT14I CCWWGG 3 cut(s) 18, 141, 592
EcoT22I ATGCAT 3 cut(s) 237, 292, 350
ErhI CCWWGG 3 cut(s) 18, 141, 592
Esp3I CGTCTC 1 cut(s) 418
FaeI CATG 8 cut(s) 145, 195, 239, 290, 457, 473, 556, 649
FatI CATG 8 cut(s) 141, 191, 235, 286, 453, 469, 552, 645
FauNDI CATATG 1 cut(s) 538
Fnu4HI GCNGC 2 cut(s) 356, 359
FokI GGATG 3 cut(s) 17, 168, 392
Fsp4HI GCNGC 2 cut(s) 356, 359
GluI GCNGC 2 cut(s) 356, 359
HaeIII GGCC 4 cut(s) 104, 355, 449, 502
Hin1II CATG 8 cut(s) 145, 195, 239, 290, 457, 473, 556, 649
HindIII AAGCTT 1 cut(s) 302
HinfI GANTC 2 cut(s) 564, 678
HphI GGTGA 2 cut(s) 308, 741
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 2 cut(s) 563, 605
Hpy188III TCNNGA 1 cut(s) 528
Hpy8I GTNNAC 1 cut(s) 210
HpyCH4IV ACGT 1 cut(s) 256
HpyCH4V TGCA 9 cut(s) 68, 235, 290, 348, 443, 545, 645, 699, 717
HpyF3I CTNAG 3 cut(s) 147, 327, 764
HpySE526I ACGT 1 cut(s) 256
Hsp92II CATG 8 cut(s) 145, 195, 239, 290, 457, 473, 556, 649
Kzo9I GATC 2 cut(s) 262, 767
LmnI GCTCC 1 cut(s) 113
LpnPI CCDG 8 cut(s) 102, 153, 180, 234, 261, 484, 676, 703
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 2 cut(s) 222, 726
MaeII ACGT 1 cut(s) 256
MaeIII GTNAC 2 cut(s) 97, 266
MalI GATC 2 cut(s) 264, 769
MboI GATC 2 cut(s) 262, 767
MboII GAAGA 3 cut(s) 48, 571, 712
MfeI CAATTG 1 cut(s) 753
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 9 cut(s) 11, 30, 48, 275, 388, 438, 546, 569, 753
MluNI TGGCCA 1 cut(s) 449
MmeI TCCRAC 2 cut(s) 91, 541
MnlI CCTC 6 cut(s) 54, 100, 336, 419, 599, 752
Mox20I TGGCCA 1 cut(s) 449
Mph1103I ATGCAT 3 cut(s) 237, 292, 350
MscI TGGCCA 1 cut(s) 449
MseI TTAA 2 cut(s) 33, 808
MslI CAYNNNNRTG 1 cut(s) 320
Msp20I TGGCCA 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 361
MspR9I CCNGG 3 cut(s) 168, 249, 691
MunI CAATTG 1 cut(s) 753
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 3 cut(s) 168, 249, 691
NcoI CCATGG 1 cut(s) 141
NdeI CATATG 1 cut(s) 538
NdeII GATC 2 cut(s) 262, 767
NlaIII CATG 8 cut(s) 145, 195, 239, 290, 457, 473, 556, 649
NlaIV GGNNCC 2 cut(s) 246, 324
NmeAIII GCCGAG 1 cut(s) 130
NsiI ATGCAT 3 cut(s) 237, 292, 350
NspI RCATGY 2 cut(s) 290, 457
PaeI GCATGC 1 cut(s) 290
PciI ACATGT 1 cut(s) 453
PcsI WCGNNNNNNNCGW 1 cut(s) 262
PctI GAATGC 1 cut(s) 70
PfeI GAWTC 2 cut(s) 564, 678
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 2 cut(s) 357, 360
PscI ACATGT 1 cut(s) 453
PshBI ATTAAT 1 cut(s) 33
Psp1406I AACGTT 1 cut(s) 256
Psp6I CCWGG 3 cut(s) 166, 247, 689
PspGI CCWGG 3 cut(s) 166, 247, 689
PspN4I GGNNCC 2 cut(s) 246, 324
PspPI GGNCC 2 cut(s) 164, 184
PvuII CAGCTG 1 cut(s) 361
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 320
SaqAI TTAA 2 cut(s) 33, 808
SatI GCNGC 2 cut(s) 356, 359
Sau3AI GATC 2 cut(s) 262, 767
Sau96I GGNCC 2 cut(s) 164, 184
ScrFI CCNGG 3 cut(s) 168, 249, 691
SetI ASST 9 cut(s) 111, 118, 153, 259, 306, 363, 485, 535, 794
SfaNI GCATC 2 cut(s) 222, 726
SinI GGWCC 2 cut(s) 164, 184
SmiMI CAYNNNNRTG 1 cut(s) 320
SphI GCATGC 1 cut(s) 290
Sse9I AATT 9 cut(s) 11, 30, 48, 275, 388, 438, 546, 569, 753
SsiI CCGC 1 cut(s) 356
StyD4I CCNGG 3 cut(s) 166, 247, 689
StyI CCWWGG 3 cut(s) 18, 141, 592
TaiI ACGT 1 cut(s) 259
TaqI TCGA 1 cut(s) 59
TaqII GACCGA 1 cut(s) 116
TasI AATT 9 cut(s) 11, 30, 48, 275, 388, 438, 546, 569, 753
TatI WGTACW 1 cut(s) 207
TauI GCSGC 1 cut(s) 358
TfiI GAWTC 2 cut(s) 564, 678
Tru1I TTAA 2 cut(s) 33, 808
Tru9I TTAA 2 cut(s) 33, 808
TscAI CASTG 2 cut(s) 494, 589
TseI GCWGC 1 cut(s) 358
TspDTI ATGAA 4 cut(s) 21, 180, 511, 525
TspRI CASTG 2 cut(s) 494, 589
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 2 cut(s) 164, 184
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 2 cut(s) 388, 569
XceI RCATGY 2 cut(s) 290, 457
Zsp2I ATGCAT 3 cut(s) 237, 292, 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.