Rw2G053900

Lysine histidine transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003493
Physical Location & Seq
Forward (+)
81621882 .. 81623912
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G053900.1

Sequence Viewer

Length: 1269 bp
ATGGATGAAAAATTAGCCAAGGAAAACGCAATTAATGAGTGGCTTCCAATTACTTCTTCGAGGAATGCAAAATGGTGGTATTCGGCTTTTCACAATGTTACGGCCATGGTTGGAGCTGGTGTTCTCGGTCTCCCTTATGCCATGGCTGAGCTTGGATGTTTTGTTAATTATTCCATGGGTCCTGGTATAGCTATTTTGGTCCTTTCATGGGTCATCACATTACACACACTATGGCAAATGGTTGAGATGCATGAGATGGTTCCAGGGAAACGTTTTGATCGTTACCATGAATTGGGTCAGCATGCATTTGGTGAAAAGCTTGGTCTTTACATCGTGGTGCCTCAGCAACTCATTGTTGAAGTTGGTGTTTGCATTGTGTACATGGTCACCGGAGGAAAATCGCTGCAGAAGGTCCATGACACTGTGTGCTCAGACTGCAAAAAGATCAAATTGACTTACTTCATCATGATCTTTGCCTCTGTTCACTTTGTGCTTTCCCACCTTCCCAACTTCAACTCCATCTCCGGCGTGTCTTTGGCCGCAGCTGTCATGTCCTTGAGTTACACTACCATAGCATGGACTGCTGCGGTAGACAAGGGTGTGGTAGAAAATGTCGATTATGGTTACAAAGCCACGACTACTACAGGAATTTTTTTTCAACTTCTTAGCTTCCTCGAAATCCAAGCTACAATTCCTTCTACACCTGAGAAGCCTTCCAAGGTACCTATGTGGAAAGGAGTCATTGTTGCCTACATAATTGTGGCTTTGTGCTACTTCCCAGTTGCTCTAGTAGGATATTACATATATGGAAATTCAAATGATGACAACATCCTCGTCTCATTGGAAAGACCCAAATGGCTAATTGTAATGGCCAACATTCTGACACTACTAGCTCTGCTGCAAATTTATGCAATGCCAGTGTTTGACATGATGGAAACTCTACTGGTAAAAAAGTGGGATTTCAGGCCAAGTTTTATGCTTCGCTTCATTACACGAGCTTCATATGTTGCAATTACCATGTTTGTCGGAATCACATTCCCTTTCTTCAGTGGTCTCCTTGGATTTTTTGGAGGATTTGCTTATTCCCCAACAACATATTTTCTCCCTTGCATAATGTGGCTTGCCATCTACAAAGCAAAGAGATTCGGCTTATCCTGGTGGTGCAACTATATCTGCATCTTCTTTGGTGTTCTTTTGATGGTTCTATCACCAATTGGAGGGCTGAGATCAATCATAGTTCAAGCCAAGACCTACGAGTTCTACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

422

Amino Acids

47.54

Weight (kDa)

8.61

Isoelectric Point (pI)

30.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 22 - 406 9.2e-85 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 721
AccB1I GGYRCC 2 cut(s) 337, 721
AccB7I CCANNNNNTGG 2 cut(s) 292, 576
AccI GTMKAC 1 cut(s) 591
AciI CCGC 2 cut(s) 540, 587
AclI AACGTT 1 cut(s) 271
AcoI YGGCCR 3 cut(s) 102, 537, 870
AcsI RAATTY 3 cut(s) 648, 811, 903
AcuI CTGAAG 1 cut(s) 1030
AdeI CACNNNGTG 2 cut(s) 426, 490
AfaI GTAC 2 cut(s) 380, 723
AfiI CCNNNNNNNGG 4 cut(s) 208, 292, 576, 1217
AgsI TTSAA 5 cut(s) 359, 514, 659, 816, 1241
AjnI CCWGG 3 cut(s) 181, 262, 1154
AjuI GAANNNNNNNTTGG 4 cut(s) 40, 72, 675, 707
AloI GAACNNNNNNTCC 2 cut(s) 105, 137
AluBI AGCT 9 cut(s) 116, 151, 191, 319, 545, 669, 686, 893, 998
AluI AGCT 9 cut(s) 116, 151, 191, 319, 545, 669, 686, 893, 998
Alw21I GWGCWC 1 cut(s) 431
Alw26I GTCTC 3 cut(s) 134, 841, 1058
AoxI GGCC 4 cut(s) 102, 537, 870, 965
ApeKI GCWGC 4 cut(s) 403, 542, 584, 898
ApoI RAATTY 3 cut(s) 648, 811, 903
AseI ATTAAT 1 cut(s) 33
Asp718I GGTACC 1 cut(s) 721
AspS9I GGNCC 3 cut(s) 179, 199, 412
AsuHPI GGTGA 3 cut(s) 323, 379, 1200
AvaII GGWCC 3 cut(s) 179, 199, 412
BaeI ACNNNNGTAYC 2 cut(s) 713, 746
BalI TGGCCA 1 cut(s) 872
BanI GGYRCC 2 cut(s) 337, 721
BauI CACGAG 1 cut(s) 993
Bbv12I GWGCWC 1 cut(s) 431
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 4 cut(s) 390, 554, 571, 885
BccI CCATC 5 cut(s) 250, 527, 925, 1133, 1192
BceAI ACGGC 1 cut(s) 117
BciT130I CCWGG 3 cut(s) 183, 264, 1156
BcoDI GTCTC 3 cut(s) 134, 841, 1058
BfaI CTAG 2 cut(s) 788, 890
BfmI CTRYAG 2 cut(s) 404, 642
BisI GCNGC 5 cut(s) 404, 540, 543, 585, 899
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 5 cut(s) 405, 541, 544, 586, 900
Bme1390I CCNGG 3 cut(s) 183, 264, 1156
Bme18I GGWCC 3 cut(s) 179, 199, 412
BmgT120I GGNCC 3 cut(s) 179, 199, 412
BmiI GGNNCC 4 cut(s) 180, 261, 339, 723
BmrFI CCNGG 3 cut(s) 183, 264, 1156
BmrI ACTGGG 1 cut(s) 773
BmsI GCATC 2 cut(s) 237, 1185
BmuI ACTGGG 1 cut(s) 773
BplI GAGNNNNNCTC 1 cut(s) 1247
Bpu10I CCTNAGC 1 cut(s) 342
Bpu1102I GCTNAGC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 577
BsaI GGTCTC 2 cut(s) 134, 1058
BsaJI CCNNGG 7 cut(s) 18, 105, 141, 174, 263, 717, 1057
BsaWI WCCGGW 1 cut(s) 389
BsaXI ACNNNNNCTCC 8 cut(s) 105, 135, 500, 506, 530, 536, 729, 759
Bsc4I CCNNNNNNNGG 4 cut(s) 208, 292, 576, 1217
Bse1I ACTGG 3 cut(s) 779, 917, 948
Bse3DI GCAATG 1 cut(s) 918
BseBI CCWGG 3 cut(s) 183, 264, 1156
BseDI CCNNGG 7 cut(s) 18, 105, 141, 174, 263, 717, 1057
BseGI GGATG 3 cut(s) 10, 161, 828
BseLI CCNNNNNNNGG 4 cut(s) 208, 292, 576, 1217
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 5 cut(s) 138, 356, 444, 696, 1214
BseNI ACTGG 3 cut(s) 779, 917, 948
BseXI GCAGC 4 cut(s) 390, 554, 571, 885
BshFI GGCC 4 cut(s) 104, 539, 872, 967
BshNI GGYRCC 2 cut(s) 337, 721
BsiHKAI GWGCWC 1 cut(s) 431
BsiSI CCGG 2 cut(s) 390, 525
BslI CCNNNNNNNGG 4 cut(s) 208, 292, 576, 1217
BsmAI GTCTC 3 cut(s) 134, 841, 1058
BsmBI CGTCTC 1 cut(s) 841
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 4 cut(s) 104, 539, 872, 967
Bso31I GGTCTC 2 cut(s) 134, 1058
Bsp1286I GDGCHC 1 cut(s) 431
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 4 cut(s) 277, 444, 468, 1226
Bsp1720I GCTNAGC 1 cut(s) 147
Bsp19I CCATGG 3 cut(s) 105, 141, 174
BspACI CCGC 2 cut(s) 540, 587
BspANI GGCC 4 cut(s) 104, 539, 872, 967
BspCNI CTCAG 5 cut(s) 139, 355, 443, 697, 1215
BspHI TCATGA 1 cut(s) 465
BspLI GGNNCC 4 cut(s) 180, 261, 339, 723
BspMAI CTGCAG 1 cut(s) 408
BspT107I GGYRCC 2 cut(s) 337, 721
BspTNI GGTCTC 2 cut(s) 134, 1058
BsrDI GCAATG 1 cut(s) 918
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 3 cut(s) 779, 917, 948
BssECI CCNNGG 7 cut(s) 18, 105, 141, 174, 263, 717, 1057
BssMI GATC 4 cut(s) 277, 444, 468, 1226
BssSI CACGAG 1 cut(s) 993
BssT1I CCWWGG 6 cut(s) 18, 105, 141, 174, 717, 1057
Bst2BI CACGAG 1 cut(s) 993
Bst2UI CCWGG 3 cut(s) 183, 264, 1156
Bst4CI ACNGT 1 cut(s) 424
BstAPI GCANNNNNTGC 1 cut(s) 581
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 303, 1122
BstDEI CTNAG 6 cut(s) 147, 342, 430, 665, 705, 1223
BstDSI CCRYGG 3 cut(s) 105, 141, 174
BstEII GGTNACC 1 cut(s) 385
BstF5I GGATG 3 cut(s) 10, 161, 828
BstKTI GATC 4 cut(s) 280, 447, 471, 1229
BstMAI GTCTC 3 cut(s) 134, 841, 1058
BstMBI GATC 4 cut(s) 277, 444, 468, 1226
BstMWI GCNNNNNNNGC 2 cut(s) 435, 581
BstNI CCWGG 3 cut(s) 183, 264, 1156
BstNSI RCATGY 1 cut(s) 305
BstPI GGTNACC 1 cut(s) 385
BstSCI CCNGG 3 cut(s) 181, 262, 1154
BstSFI CTRYAG 2 cut(s) 404, 642
BstV1I GCAGC 4 cut(s) 390, 554, 571, 885
BsuRI GGCC 4 cut(s) 104, 539, 872, 967
BtgI CCRYGG 3 cut(s) 105, 141, 174
BtsCI GGATG 3 cut(s) 10, 161, 828
BtsIMutI CAGTG 3 cut(s) 420, 924, 1054
Cac8I GCNNGC 2 cut(s) 303, 1122
CciI TCATGA 1 cut(s) 465
Cfr13I GGNCC 3 cut(s) 179, 199, 412
Csp6I GTAC 2 cut(s) 379, 722
CviQI GTAC 2 cut(s) 379, 722
DdeI CTNAG 6 cut(s) 147, 342, 430, 665, 705, 1223
DpnI GATC 4 cut(s) 279, 446, 470, 1228
DpnII GATC 4 cut(s) 277, 444, 468, 1226
DraIII CACNNNGTG 2 cut(s) 426, 490
EaeI YGGCCR 3 cut(s) 102, 537, 870
Eco130I CCWWGG 6 cut(s) 18, 105, 141, 174, 717, 1057
Eco31I GGTCTC 2 cut(s) 134, 1058
Eco47I GGWCC 3 cut(s) 179, 199, 412
Eco57I CTGAAG 1 cut(s) 1030
Eco91I GGTNACC 1 cut(s) 385
EcoO109I RGGNCCY 1 cut(s) 179
EcoO65I GGTNACC 1 cut(s) 385
EcoRII CCWGG 3 cut(s) 181, 262, 1154
EcoT14I CCWWGG 6 cut(s) 18, 105, 141, 174, 717, 1057
EcoT22I ATGCAT 2 cut(s) 252, 307
ErhI CCWWGG 6 cut(s) 18, 105, 141, 174, 717, 1057
Esp3I CGTCTC 1 cut(s) 841
FauNDI CATATG 1 cut(s) 1003
FblI GTMKAC 1 cut(s) 591
Fnu4HI GCNGC 5 cut(s) 404, 540, 543, 585, 899
FokI GGATG 3 cut(s) 17, 168, 815
Fsp4HI GCNGC 5 cut(s) 404, 540, 543, 585, 899
FspBI CTAG 2 cut(s) 788, 890
GluI GCNGC 5 cut(s) 404, 540, 543, 585, 899
HaeIII GGCC 4 cut(s) 104, 539, 872, 967
HapII CCGG 2 cut(s) 390, 525
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 3 cut(s) 738, 1029, 1143
HpaII CCGG 2 cut(s) 390, 525
HphI GGTGA 3 cut(s) 323, 379, 1200
Hpy166II GTNNAC 3 cut(s) 379, 484, 592
Hpy188I TCNGA 3 cut(s) 433, 882, 1028
Hpy188III TCNNGA 1 cut(s) 466
Hpy8I GTNNAC 3 cut(s) 379, 484, 592
HpyAV CCTTC 4 cut(s) 403, 512, 705, 723
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 1 cut(s) 271
HpyF10VI GCNNNNNNNGC 2 cut(s) 435, 581
HpyF3I CTNAG 6 cut(s) 147, 342, 430, 665, 705, 1223
HpySE526I ACGT 1 cut(s) 271
KpnI GGTACC 1 cut(s) 725
Kzo9I GATC 4 cut(s) 277, 444, 468, 1226
LmnI GCTCC 1 cut(s) 113
Lsp1109I GCAGC 4 cut(s) 390, 554, 571, 885
LweI GCATC 2 cut(s) 237, 1185
MaeI CTAG 2 cut(s) 788, 890
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 5 cut(s) 97, 281, 385, 560, 623
MalI GATC 4 cut(s) 279, 446, 470, 1228
MboI GATC 4 cut(s) 277, 444, 468, 1226
MboII GAAGA 3 cut(s) 48, 1036, 1171
MfeI CAATTG 1 cut(s) 1212
MhlI GDGCHC 1 cut(s) 431
MlsI TGGCCA 1 cut(s) 872
MluNI TGGCCA 1 cut(s) 872
MlyI GAGTC 1 cut(s) 747
MmeI TCCRAC 2 cut(s) 91, 1006
MnlI CCTC 8 cut(s) 54, 351, 386, 487, 683, 842, 1064, 1211
Mox20I TGGCCA 1 cut(s) 872
Mph1103I ATGCAT 2 cut(s) 252, 307
MscI TGGCCA 1 cut(s) 872
MseI TTAA 3 cut(s) 33, 165, 1267
MslI CAYNNNNRTG 2 cut(s) 335, 758
Msp20I TGGCCA 1 cut(s) 872
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 2 cut(s) 390, 525
MspR9I CCNGG 3 cut(s) 183, 264, 1156
MunI CAATTG 1 cut(s) 1212
Mva1269I GAATGC 1 cut(s) 70
MvaI CCWGG 3 cut(s) 183, 264, 1156
MwoI GCNNNNNNNGC 2 cut(s) 435, 581
NcoI CCATGG 3 cut(s) 105, 141, 174
NdeI CATATG 1 cut(s) 1003
NdeII GATC 4 cut(s) 277, 444, 468, 1226
NlaIV GGNNCC 4 cut(s) 180, 261, 339, 723
NmuCI GTSAC 1 cut(s) 385
NsiI ATGCAT 2 cut(s) 252, 307
NspI RCATGY 1 cut(s) 305
PaeI GCATGC 1 cut(s) 305
PagI TCATGA 1 cut(s) 465
PcsI WCGNNNNNNNCGW 1 cut(s) 277
PctI GAATGC 1 cut(s) 70
PfeI GAWTC 2 cut(s) 1029, 1143
PflMI CCANNNNNTGG 2 cut(s) 292, 576
PkrI GCNGC 5 cut(s) 405, 541, 544, 586, 900
PleI GAGTC 1 cut(s) 746
PpsI GAGTC 1 cut(s) 746
PpuMI RGGWCCY 1 cut(s) 179
PshBI ATTAAT 1 cut(s) 33
Psp1406I AACGTT 1 cut(s) 271
Psp5II RGGWCCY 1 cut(s) 179
Psp6I CCWGG 3 cut(s) 181, 262, 1154
PspEI GGTNACC 1 cut(s) 385
PspGI CCWGG 3 cut(s) 181, 262, 1154
PspN4I GGNNCC 4 cut(s) 180, 261, 339, 723
PspPI GGNCC 3 cut(s) 179, 199, 412
PspPPI RGGWCCY 1 cut(s) 179
PstI CTGCAG 1 cut(s) 408
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 2 cut(s) 380, 723
RsaNI GTAC 2 cut(s) 379, 722
RseI CAYNNNNRTG 2 cut(s) 335, 758
SaqAI TTAA 3 cut(s) 33, 165, 1267
SatI GCNGC 5 cut(s) 404, 540, 543, 585, 899
Sau3AI GATC 4 cut(s) 277, 444, 468, 1226
Sau96I GGNCC 3 cut(s) 179, 199, 412
SchI GAGTC 1 cut(s) 747
ScrFI CCNGG 3 cut(s) 183, 264, 1156
SduI GDGCHC 1 cut(s) 431
SfaNI GCATC 2 cut(s) 237, 1185
SfcI CTRYAG 2 cut(s) 404, 642
SinI GGWCC 3 cut(s) 179, 199, 412
SmiMI CAYNNNNRTG 2 cut(s) 335, 758
SmlI CTYRAG 1 cut(s) 556
SmoI CTYRAG 1 cut(s) 556
SphI GCATGC 1 cut(s) 305
SsiI CCGC 2 cut(s) 540, 587
SspMI CTAG 2 cut(s) 788, 890
StyD4I CCNGG 3 cut(s) 181, 262, 1154
StyI CCWWGG 6 cut(s) 18, 105, 141, 174, 717, 1057
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 1 cut(s) 274
TaqI TCGA 3 cut(s) 59, 615, 675
TaqII GACCGA 1 cut(s) 116
TatI WGTACW 1 cut(s) 378
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 2 cut(s) 1029, 1143
Tru1I TTAA 3 cut(s) 33, 165, 1267
Tru9I TTAA 3 cut(s) 33, 165, 1267
TscAI CASTG 3 cut(s) 427, 924, 1054
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 4 cut(s) 403, 542, 584, 898
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 6 cut(s) 21, 195, 303, 451, 976, 990
TspRI CASTG 3 cut(s) 427, 924, 1054
Van91I CCANNNNNTGG 2 cut(s) 292, 576
VpaK11BI GGWCC 3 cut(s) 179, 199, 412
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 3 cut(s) 648, 811, 903
XceI RCATGY 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 591
XspI CTAG 2 cut(s) 788, 890
Zsp2I ATGCAT 2 cut(s) 252, 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.