pycom11g00660

Serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0002422
Physical Location & Seq
Reverse (-)
491188 .. 495744
4557 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGGATTCGGTCCCTTCGAACTCCCATGGGAATCTGGATGAGCAGATTGCTCAACTCATGCAGTGCAAGCCCTTGTCCGAGCAAGAGGTAAGGGTGCTCTGTGAGAAGGCCAAGGAAATATTAATGGAAGAAAGCAATGTCCAGCCTGTAAAAAGCCCTGTAACTATCTGTGGTGATATTCATGGACAATTCCATGATCTAGCGGAGCTTTTCCGCATTGGAGGAAAGTGCCCAGATACAAACTATTTATTTATGGGAGATTATGTGGACCGTGGCTATTATTCAGTTGAAACAGTGACCCTCCTTGTGGCCTTGAAGGTGCGTTATCATCAGCGTATTACTATCCTAAGAGGAAACCATGAAAGCCGTCAGATTACTCAAGTTTATGGTTTTTATGACGAGTGCCTACGGAAGTATGGTAATGCCAATGTTTGGAAGATCTTTACAGATTTATTTGACTATTTCCCGCTCACAGCATTGGTTGAATCTGAAATATTCTGCCTGCATGGAGGGCTGTCTCCATCCATTGAAACCCTCGATAACATACGTAATTTGACCGTGTACAAGAGGTTCCTCATGAGGGTCCCATGTGTGATCTTTTATGGTCTGATCATGATCGATCGCTGTGGTTGGGGTATCTCTCCGAGGGGTGCTGGATATACATTTGGCCAGGACATATCCGAGCAGTTTAATCATACAAATAACTTAAAGTTGATTGCTAGAGCACATCAGCTGGTCATGGAAGGATACAACTGGGGTCATGAACAAAAAGTGGTCACTATATTTAGTGCACCCAATTATTGTTATCGCTGTGGAAATATGGCATCCATCCTGGAAGTCGATGACTGCAAAGGTCACACATTCATTCAGTTTGAGCCAGCTCCAAGGAGGGGAGAACCGGATGTGACCCGAAGAACACCTGATTACTTCCTATAA

Protein Analysis

312

Amino Acids

35.71

Weight (kDa)

5.94

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 53 - 246 2.5e-39 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 432
AccBSI CCGCTC 1 cut(s) 469
AciI CCGC 3 cut(s) 203, 214, 467
AcoI YGGCCR 1 cut(s) 667
AfaI GTAC 1 cut(s) 563
AfiI CCNNNNNNNGG 4 cut(s) 307, 432, 580, 890
AgsI TTSAA 4 cut(s) 290, 316, 485, 530
AjnI CCWGG 2 cut(s) 669, 831
AluBI AGCT 3 cut(s) 208, 733, 881
AluI AGCT 3 cut(s) 208, 733, 881
Alw21I GWGCWC 3 cut(s) 99, 727, 793
Alw26I GTCTC 1 cut(s) 522
Alw44I GTGCAC 1 cut(s) 789
AoxI GGCC 3 cut(s) 108, 309, 667
ApaLI GTGCAC 1 cut(s) 789
AseI ATTAAT 1 cut(s) 122
AspS9I GGNCC 3 cut(s) 10, 268, 583
AsuHPI GGTGA 1 cut(s) 185
AsuII TTCGAA 1 cut(s) 17
AvaII GGWCC 3 cut(s) 10, 268, 583
BaeGI GKGCMC 2 cut(s) 233, 793
BalI TGGCCA 1 cut(s) 669
Bbv12I GWGCWC 3 cut(s) 99, 727, 793
BccI CCATC 2 cut(s) 529, 836
BceAI ACGGC 1 cut(s) 351
BciT130I CCWGG 2 cut(s) 671, 833
BciVI GTATCC 1 cut(s) 740
BclI TGATCA 1 cut(s) 609
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 2 cut(s) 200, 720
BfuI GTATCC 1 cut(s) 740
BglII AGATCT 1 cut(s) 438
Bme1390I CCNGG 2 cut(s) 671, 833
Bme18I GGWCC 3 cut(s) 10, 268, 583
BmgT120I GGNCC 3 cut(s) 10, 268, 583
BmiI GGNNCC 4 cut(s) 12, 572, 584, 585
BmrFI CCNGG 2 cut(s) 671, 833
BmrI ACTGGG 1 cut(s) 763
BmsI GCATC 1 cut(s) 833
BmuI ACTGGG 1 cut(s) 763
Bpu14I TTCGAA 1 cut(s) 17
BpuEI CTTGAG 1 cut(s) 363
Bsa29I ATCGAT 1 cut(s) 618
BsaAI YACGTR 1 cut(s) 548
BsaBI GATNNNNATC 1 cut(s) 614
BsaJI CCNNGG 5 cut(s) 25, 111, 271, 644, 884
BsaWI WCCGGW 1 cut(s) 898
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 432, 580, 890
Bse1I ACTGG 1 cut(s) 758
Bse3DI GCAATG 1 cut(s) 142
Bse8I GATNNNNATC 1 cut(s) 614
BseBI CCWGG 2 cut(s) 671, 833
BseCI ATCGAT 1 cut(s) 618
BseDI CCNNGG 5 cut(s) 25, 111, 271, 644, 884
BseGI GGATG 5 cut(s) 43, 521, 824, 828, 907
BseJI GATNNNNATC 1 cut(s) 614
BseLI CCNNNNNNNGG 4 cut(s) 307, 432, 580, 890
BseMI GCAATG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 758
BseSI GKGCMC 2 cut(s) 233, 793
Bsh1285I CGRYCG 1 cut(s) 622
BshFI GGCC 3 cut(s) 110, 311, 669
BshVI ATCGAT 1 cut(s) 618
BsiEI CGRYCG 1 cut(s) 622
BsiHKAI GWGCWC 3 cut(s) 99, 727, 793
BsiSI CCGG 1 cut(s) 899
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 4 cut(s) 307, 432, 580, 890
BsmAI GTCTC 1 cut(s) 522
BsmFI GGGAC 1 cut(s) 569
BsnI GGCC 3 cut(s) 110, 311, 669
Bsp119I TTCGAA 1 cut(s) 17
Bsp1286I GDGCHC 4 cut(s) 99, 233, 727, 793
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 6 cut(s) 196, 438, 594, 609, 615, 619
Bsp19I CCATGG 1 cut(s) 25
BspACI CCGC 3 cut(s) 203, 214, 467
BspANI GGCC 3 cut(s) 110, 311, 669
BspDI ATCGAT 1 cut(s) 618
BspHI TCATGA 3 cut(s) 576, 612, 760
BspLI GGNNCC 4 cut(s) 12, 572, 584, 585
BspT104I TTCGAA 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 469
BsrDI GCAATG 1 cut(s) 142
BsrGI TGTACA 1 cut(s) 561
BsrI ACTGG 1 cut(s) 758
BssECI CCNNGG 5 cut(s) 25, 111, 271, 644, 884
BssMI GATC 6 cut(s) 196, 438, 594, 609, 615, 619
BssT1I CCWWGG 3 cut(s) 25, 111, 884
Bst2UI CCWGG 2 cut(s) 671, 833
Bst4CI ACNGT 3 cut(s) 272, 295, 559
BstAUI TGTACA 1 cut(s) 561
BstBAI YACGTR 1 cut(s) 548
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 3 cut(s) 68, 503, 879
BstDEI CTNAG 1 cut(s) 347
BstDSI CCRYGG 2 cut(s) 25, 271
BstF5I GGATG 5 cut(s) 43, 521, 824, 828, 907
BstKTI GATC 6 cut(s) 199, 441, 597, 612, 618, 622
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 6 cut(s) 196, 438, 594, 609, 615, 619
BstMCI CGRYCG 1 cut(s) 622
BstMWI GCNNNNNNNGC 2 cut(s) 67, 511
BstNI CCWGG 2 cut(s) 671, 833
BstSCI CCNGG 2 cut(s) 669, 831
BstSLI GKGCMC 2 cut(s) 233, 793
BstSNI TACGTA 1 cut(s) 548
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
Bsu15I ATCGAT 1 cut(s) 618
BsuI GTATCC 1 cut(s) 740
BsuRI GGCC 3 cut(s) 110, 311, 669
BsuTUI ATCGAT 1 cut(s) 618
BtgI CCRYGG 2 cut(s) 25, 271
BtsCI GGATG 5 cut(s) 43, 521, 824, 828, 907
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 2 cut(s) 68, 300
Cac8I GCNNGC 3 cut(s) 68, 503, 879
CciI TCATGA 3 cut(s) 576, 612, 760
Cfr13I GGNCC 3 cut(s) 10, 268, 583
ClaI ATCGAT 1 cut(s) 618
Csp6I GTAC 1 cut(s) 562
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 1 cut(s) 347
DpnI GATC 6 cut(s) 198, 440, 596, 611, 617, 621
DpnII GATC 6 cut(s) 196, 438, 594, 609, 615, 619
EaeI YGGCCR 1 cut(s) 667
Eco105I TACGTA 1 cut(s) 548
Eco130I CCWWGG 3 cut(s) 25, 111, 884
Eco47I GGWCC 3 cut(s) 10, 268, 583
EcoO109I RGGNCCY 1 cut(s) 583
EcoRII CCWGG 2 cut(s) 669, 831
EcoT14I CCWWGG 3 cut(s) 25, 111, 884
ErhI CCWWGG 3 cut(s) 25, 111, 884
FaqI GGGAC 1 cut(s) 569
FauI CCCGC 1 cut(s) 474
FbaI TGATCA 1 cut(s) 609
FokI GGATG 5 cut(s) 50, 508, 811, 815, 914
FspBI CTAG 2 cut(s) 200, 720
HaeIII GGCC 3 cut(s) 110, 311, 669
HapII CCGG 1 cut(s) 899
HinfI GANTC 3 cut(s) 5, 31, 485
HpaII CCGG 1 cut(s) 899
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 3 cut(s) 268, 562, 791
Hpy188I TCNGA 6 cut(s) 79, 372, 490, 609, 645, 682
Hpy188III TCNNGA 4 cut(s) 35, 577, 613, 761
Hpy8I GTNNAC 3 cut(s) 268, 562, 791
HpyAV CCTTC 4 cut(s) 24, 100, 310, 737
HpyCH4III ACNGT 3 cut(s) 272, 295, 559
HpyCH4IV ACGT 1 cut(s) 547
HpyCH4V TGCA 5 cut(s) 61, 66, 505, 791, 849
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 511
HpyF3I CTNAG 1 cut(s) 347
HpySE526I ACGT 1 cut(s) 547
KflI GGGWCCC 1 cut(s) 583
Ksp22I TGATCA 1 cut(s) 609
Kzo9I GATC 6 cut(s) 196, 438, 594, 609, 615, 619
LmnI GCTCC 2 cut(s) 205, 886
LweI GCATC 1 cut(s) 833
MaeI CTAG 2 cut(s) 200, 720
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 5 cut(s) 160, 295, 775, 854, 904
MalI GATC 6 cut(s) 198, 440, 596, 611, 617, 621
MbiI CCGCTC 1 cut(s) 469
MboI GATC 6 cut(s) 196, 438, 594, 609, 615, 619
MboII GAAGA 3 cut(s) 140, 448, 924
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 4 cut(s) 99, 233, 727, 793
MlsI TGGCCA 1 cut(s) 669
MluCI AATT 3 cut(s) 188, 550, 796
MluNI TGGCCA 1 cut(s) 669
Mox20I TGGCCA 1 cut(s) 669
MscI TGGCCA 1 cut(s) 669
MseI TTAA 3 cut(s) 122, 690, 707
Msp20I TGGCCA 1 cut(s) 669
MspA1I CMGCKG 1 cut(s) 733
MspI CCGG 1 cut(s) 899
MspR9I CCNGG 2 cut(s) 671, 833
MvaI CCWGG 2 cut(s) 671, 833
MwoI GCNNNNNNNGC 2 cut(s) 67, 511
NcoI CCATGG 1 cut(s) 25
NdeII GATC 6 cut(s) 196, 438, 594, 609, 615, 619
NlaIV GGNNCC 4 cut(s) 12, 572, 584, 585
NmuCI GTSAC 4 cut(s) 295, 775, 854, 904
NspV TTCGAA 1 cut(s) 17
PagI TCATGA 3 cut(s) 576, 612, 760
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 3 cut(s) 5, 31, 485
PflMI CCANNNNNTGG 1 cut(s) 432
PfoI TCCNGGA 1 cut(s) 831
Ple19I CGATCG 1 cut(s) 622
Ppu21I YACGTR 1 cut(s) 548
PpuMI RGGWCCY 1 cut(s) 583
PshBI ATTAAT 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 583
Psp6I CCWGG 2 cut(s) 669, 831
PspGI CCWGG 2 cut(s) 669, 831
PspN4I GGNNCC 4 cut(s) 12, 572, 584, 585
PspPI GGNCC 3 cut(s) 10, 268, 583
PspPPI RGGWCCY 1 cut(s) 583
PsrI GAACNNNNNNTAC 2 cut(s) 554, 586
PsuI RGATCY 1 cut(s) 438
PvuI CGATCG 1 cut(s) 622
PvuII CAGCTG 1 cut(s) 733
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
SaqAI TTAA 3 cut(s) 122, 690, 707
Sau3AI GATC 6 cut(s) 196, 438, 594, 609, 615, 619
Sau96I GGNCC 3 cut(s) 10, 268, 583
ScrFI CCNGG 2 cut(s) 671, 833
SduI GDGCHC 4 cut(s) 99, 233, 727, 793
SetI ASST 9 cut(s) 90, 210, 321, 550, 572, 735, 856, 883, 922
SfaNI GCATC 1 cut(s) 833
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 3 cut(s) 10, 268, 583
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
SnaBI TACGTA 1 cut(s) 548
Sse9I AATT 3 cut(s) 188, 550, 796
SsiI CCGC 3 cut(s) 203, 214, 467
SspI AATATT 2 cut(s) 120, 495
SspMI CTAG 2 cut(s) 200, 720
StyD4I CCNGG 2 cut(s) 669, 831
StyI CCWWGG 3 cut(s) 25, 111, 884
TaaI ACNGT 3 cut(s) 272, 295, 559
TaiI ACGT 1 cut(s) 550
TaqI TCGA 4 cut(s) 17, 537, 618, 840
TasI AATT 3 cut(s) 188, 550, 796
TatI WGTACW 1 cut(s) 561
TfiI GAWTC 3 cut(s) 5, 31, 485
Tru1I TTAA 3 cut(s) 122, 690, 707
Tru9I TTAA 3 cut(s) 122, 690, 707
TscAI CASTG 2 cut(s) 68, 300
TseFI GTSAC 4 cut(s) 295, 775, 854, 904
Tsp45I GTSAC 4 cut(s) 295, 775, 854, 904
TspDTI ATGAA 4 cut(s) 170, 375, 777, 853
TspGWI ACGGA 1 cut(s) 424
TspRI CASTG 2 cut(s) 68, 300
Van91I CCANNNNNTGG 1 cut(s) 432
VneI GTGCAC 1 cut(s) 789
VpaK11BI GGWCC 3 cut(s) 10, 268, 583
VspI ATTAAT 1 cut(s) 122
XspI CTAG 2 cut(s) 200, 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.