pycom11g22430

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003786
Physical Location & Seq
Reverse (-)
25431691 .. 25432445
755 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
ATGGCTCCGACGACGGAGAAGTCCCCACCCAAATCCGGCTCTGAGGACCCGAACCCGACATCCAACGAGACCCGATTCCGGGGTGTCCGAAAGAGGCCTTGGGGACGCTACGCCGCCGAGATTAGAGATCCCGGGAAGAAGACCCGCGTCTGGCTCGGGACCTTCGACACCGCTGAGGAGGCAGCGTGCGCCTACGACAAGGCGGCGCGTGAATTCCGCGGAGGCAAGGCGAAGACCAACTTCCCCACCCCCACTGAGCTCCATCTCGACGCCGTCAACATCGCCAACATGAACAACGCCGCCAAATTAGCTCACCGTGGAGTCCTCCTCCCCGCCGTCTCCGCGCCCGCTCGACCTCACTCTCAAAACCCCCCGCTTGTCCACCCGCGGCGGCTACTTCACCGCCGCGAGCGGCTTCCGCCCGCTTCCCGCTTCCCGTCCCGTGATCTTCTTCGACGCCCTCCAGCACGCCCGCGAAACTTGCAGGTTCGACCCCCCCGCCCCCACATCAGGCTCGGGCCACAGCGACTCGTCCTCGTCCTCGGTCGTCGACTTCGAGCGCACCTCCCGCAACGGGCGGCTCGATCTCGACCTCAACCTTCCTCCCCCCTCAGAAGTCGCCTAAACTACCGCCGCCCGCCGCGAAAGATCCCCCCTTTTTTCTTTCCGGTCCAAGTTTGTTCCTTTTTATGTAAGAAACAAAACCCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009737 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009873 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010104 GO:0010105 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016604 GO:0019219 GO:0019222 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032870 GO:0033993 GO:0035556 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070297 GO:0070298 GO:0070887 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071495 GO:0080090 GO:0080134 GO:0097159 GO:0097305 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

238

Amino Acids

26.92

Weight (kDa)

11.84

Isoelectric Point (pI)

75.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 24 - 74 5.8e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 475
AccBSI CCGCTC 2 cut(s) 350, 412
AccI GTMKAC 1 cut(s) 550
AccII CGCG 8 cut(s) 147, 208, 219, 344, 388, 408, 475, 643
AclWI GGATC 2 cut(s) 122, 643
AcsI RAATTY 1 cut(s) 212
AcyI GRCGYC 2 cut(s) 270, 457
AfiI CCNNNNNNNGG 6 cut(s) 35, 78, 79, 150, 510, 574
AjuI GAANNNNNNNTTGG 2 cut(s) 82, 114
AloI GAACNNNNNNTCC 2 cut(s) 44, 76
AluBI AGCT 2 cut(s) 259, 311
AluI AGCT 2 cut(s) 259, 311
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 62, 343
AlwI GGATC 2 cut(s) 122, 643
Ama87I CYCGRG 3 cut(s) 131, 155, 515
AoxI GGCC 2 cut(s) 95, 518
ApeKI GCWGC 1 cut(s) 182
ApoI RAATTY 1 cut(s) 212
AspLEI GCGC 4 cut(s) 191, 208, 346, 562
AspS9I GGNCC 4 cut(s) 46, 159, 518, 670
AsuC2I CCSGG 3 cut(s) 80, 132, 133
AsuHPI GGTGA 2 cut(s) 305, 392
AvaI CYCGRG 3 cut(s) 131, 155, 515
AvaII GGWCC 3 cut(s) 46, 159, 670
BanII GRGCYC 1 cut(s) 261
BbsI GAAGAC 2 cut(s) 146, 239
Bbv12I GWGCWC 1 cut(s) 261
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 194
BccI CCATC 1 cut(s) 270
BceAI ACGGC 2 cut(s) 257, 320
BcnI CCSGG 3 cut(s) 80, 132, 133
BcoDI GTCTC 2 cut(s) 62, 343
BfuAI ACCTGC 1 cut(s) 475
Bme1390I CCNGG 3 cut(s) 80, 132, 133
Bme18I GGWCC 3 cut(s) 46, 159, 670
BmeT110I CYCGRG 3 cut(s) 131, 155, 515
BmgT120I GGNCC 4 cut(s) 46, 159, 518, 670
BmiI GGNNCC 3 cut(s) 6, 48, 160
BmrFI CCNGG 3 cut(s) 80, 132, 133
BoxI GACNNNNGTC 1 cut(s) 146
BpiI GAAGAC 2 cut(s) 146, 239
BplI GAGNNNNNCTC 4 cut(s) 312, 344, 549, 581
BpmI CTGGAG 1 cut(s) 447
Bpu10I CCTNAGC 1 cut(s) 174
BpuMI CCSGG 3 cut(s) 80, 132, 133
BsaHI GRCGYC 2 cut(s) 270, 457
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 7 cut(s) 79, 98, 131, 217, 316, 386, 541
BsaWI WCCGGW 1 cut(s) 667
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 6 cut(s) 35, 78, 79, 150, 510, 574
BseDI CCNNGG 7 cut(s) 79, 98, 131, 217, 316, 386, 541
BseGI GGATG 1 cut(s) 59
BseLI CCNNNNNNNGG 6 cut(s) 35, 78, 79, 150, 510, 574
BseMII CTCAG 4 cut(s) 33, 165, 246, 625
BseRI GAGGAG 2 cut(s) 191, 317
BseXI GCAGC 1 cut(s) 194
Bsh1236I CGCG 8 cut(s) 147, 208, 219, 344, 388, 408, 475, 643
Bsh1285I CGRYCG 1 cut(s) 547
BshFI GGCC 2 cut(s) 97, 520
BsiEI CGRYCG 1 cut(s) 547
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 3 cut(s) 131, 155, 515
BsiSI CCGG 4 cut(s) 36, 79, 132, 668
BslFI GGGAC 4 cut(s) 7, 117, 172, 424
BslI CCNNNNNNNGG 6 cut(s) 35, 78, 79, 150, 510, 574
BsmAI GTCTC 2 cut(s) 62, 343
BsmBI CGTCTC 1 cut(s) 343
BsmFI GGGAC 4 cut(s) 7, 117, 172, 424
BsnI GGCC 2 cut(s) 97, 520
Bso31I GGTCTC 1 cut(s) 62
BsoBI CYCGRG 3 cut(s) 131, 155, 515
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 4 cut(s) 127, 445, 584, 648
BspANI GGCC 2 cut(s) 97, 520
BspCNI CTCAG 4 cut(s) 34, 166, 247, 624
BspFNI CGCG 8 cut(s) 147, 208, 219, 344, 388, 408, 475, 643
BspLI GGNNCC 3 cut(s) 6, 48, 160
BspMI ACCTGC 1 cut(s) 475
BspPI GGATC 2 cut(s) 122, 643
BspTNI GGTCTC 1 cut(s) 62
BsrBI CCGCTC 2 cut(s) 350, 412
BssECI CCNNGG 7 cut(s) 79, 98, 131, 217, 316, 386, 541
BssMI GATC 4 cut(s) 127, 445, 584, 648
BssNI GRCGYC 2 cut(s) 270, 457
BssT1I CCWWGG 1 cut(s) 98
Bst4CI ACNGT 1 cut(s) 317
BstACI GRCGYC 2 cut(s) 270, 457
BstC8I GCNNGC 7 cut(s) 187, 348, 410, 423, 469, 473, 638
BstDEI CTNAG 5 cut(s) 42, 174, 255, 611, 711
BstDSI CCRYGG 3 cut(s) 217, 316, 386
BstF5I GGATG 1 cut(s) 59
BstFNI CGCG 8 cut(s) 147, 208, 219, 344, 388, 408, 475, 643
BstHHI GCGC 4 cut(s) 191, 208, 346, 562
BstKTI GATC 4 cut(s) 130, 448, 587, 651
BstMAI GTCTC 2 cut(s) 62, 343
BstMBI GATC 4 cut(s) 127, 445, 584, 648
BstMCI CGRYCG 1 cut(s) 547
BstMWI GCNNNNNNNGC 7 cut(s) 179, 188, 308, 341, 418, 481, 568
BstPAI GACNNNNGTC 1 cut(s) 146
BstSCI CCNGG 3 cut(s) 78, 130, 131
BstUI CGCG 8 cut(s) 147, 208, 219, 344, 388, 408, 475, 643
BstV1I GCAGC 1 cut(s) 194
BstV2I GAAGAC 2 cut(s) 146, 239
BstX2I RGATCY 2 cut(s) 127, 648
BstYI RGATCY 2 cut(s) 127, 648
BsuRI GGCC 2 cut(s) 97, 520
BtgI CCRYGG 3 cut(s) 217, 316, 386
BtgZI GCGATG 1 cut(s) 265
BtsCI GGATG 1 cut(s) 59
BtsIMutI CAGTG 1 cut(s) 252
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 7 cut(s) 187, 348, 410, 423, 469, 473, 638
CfoI GCGC 4 cut(s) 191, 208, 346, 562
Cfr13I GGNCC 4 cut(s) 46, 159, 518, 670
Cfr42I CCGCGG 2 cut(s) 220, 389
Cfr9I CCCGGG 1 cut(s) 131
CseI GACGC 4 cut(s) 114, 136, 278, 465
CviAII CATG 1 cut(s) 289
DdeI CTNAG 5 cut(s) 42, 174, 255, 611, 711
DpnI GATC 4 cut(s) 129, 447, 586, 650
DpnII GATC 4 cut(s) 127, 445, 584, 648
DrdI GACNNNNNNGTC 1 cut(s) 19
DseDI GACNNNNNNGTC 1 cut(s) 19
EciI GGCGGA 1 cut(s) 408
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 98
Eco147I AGGCCT 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 261
Eco31I GGTCTC 1 cut(s) 62
Eco47I GGWCC 3 cut(s) 46, 159, 670
Eco53kI GAGCTC 1 cut(s) 259
Eco88I CYCGRG 3 cut(s) 131, 155, 515
EcoICRI GAGCTC 1 cut(s) 259
EcoO109I RGGNCCY 2 cut(s) 46, 159
EcoRI GAATTC 1 cut(s) 212
EcoT14I CCWWGG 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 98
Esp3I CGTCTC 1 cut(s) 343
FaeI CATG 1 cut(s) 292
FaiI YATR 2 cut(s) 290, 691
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FaqI GGGAC 4 cut(s) 7, 117, 172, 424
FatI CATG 1 cut(s) 288
FblI GTMKAC 1 cut(s) 550
FokI GGATG 1 cut(s) 46
FriOI GRGCYC 1 cut(s) 261
GlaI GCGC 4 cut(s) 190, 207, 345, 561
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 2 cut(s) 97, 520
HapII CCGG 4 cut(s) 36, 79, 132, 668
HgaI GACGC 4 cut(s) 114, 136, 278, 465
HhaI GCGC 4 cut(s) 191, 208, 346, 562
Hin1I GRCGYC 2 cut(s) 270, 457
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 4 cut(s) 189, 206, 344, 560
HinP1I GCGC 4 cut(s) 189, 206, 344, 560
HincII GTYRAC 2 cut(s) 277, 551
HindII GTYRAC 2 cut(s) 277, 551
HinfI GANTC 3 cut(s) 75, 321, 528
HpaII CCGG 4 cut(s) 36, 79, 132, 668
HphI GGTGA 2 cut(s) 305, 392
Hpy166II GTNNAC 3 cut(s) 277, 382, 551
Hpy188I TCNGA 4 cut(s) 9, 43, 89, 614
Hpy188III TCNNGA 3 cut(s) 157, 266, 588
Hpy8I GTNNAC 3 cut(s) 277, 382, 551
Hpy99I CGWCG 5 cut(s) 13, 16, 272, 459, 552
HpyAV CCTTC 2 cut(s) 172, 609
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4V TGCA 1 cut(s) 484
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 188, 308, 341, 418, 481, 568
HpyF3I CTNAG 5 cut(s) 42, 174, 255, 611, 711
Hsp92I GRCGYC 2 cut(s) 270, 457
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 4 cut(s) 189, 206, 344, 560
KspI CCGCGG 2 cut(s) 220, 389
Kzo9I GATC 4 cut(s) 127, 445, 584, 648
LmnI GCTCC 2 cut(s) 10, 264
LpnPI CCDG 8 cut(s) 49, 92, 136, 145, 470, 477, 496, 681
Lsp1109I GCAGC 1 cut(s) 194
MalI GATC 4 cut(s) 129, 447, 586, 650
MbiI CCGCTC 2 cut(s) 350, 412
MboI GATC 4 cut(s) 127, 445, 584, 648
MboII GAAGA 5 cut(s) 148, 151, 244, 440, 443
MflI RGATCY 2 cut(s) 127, 648
MhlI GDGCHC 1 cut(s) 261
MluCI AATT 2 cut(s) 212, 305
MlyI GAGTC 2 cut(s) 330, 522
MmeI TCCRAC 2 cut(s) 32, 87
MspA1I CMGCKG 3 cut(s) 173, 219, 388
MspI CCGG 4 cut(s) 36, 79, 132, 668
MspR9I CCNGG 3 cut(s) 80, 132, 133
MvnI CGCG 8 cut(s) 147, 208, 219, 344, 388, 408, 475, 643
MwoI GCNNNNNNNGC 7 cut(s) 179, 188, 308, 341, 418, 481, 568
NciI CCSGG 3 cut(s) 80, 132, 133
NdeII GATC 4 cut(s) 127, 445, 584, 648
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 3 cut(s) 6, 48, 160
NmeAIII GCCGAG 1 cut(s) 142
PceI AGGCCT 1 cut(s) 97
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 553, 580
PfeI GAWTC 1 cut(s) 75
PflFI GACNNNGTC 1 cut(s) 272
PleI GAGTC 2 cut(s) 329, 522
PpsI GAGTC 2 cut(s) 329, 522
PpuMI RGGWCCY 2 cut(s) 46, 159
PshAI GACNNNNGTC 1 cut(s) 146
Psp124BI GAGCTC 1 cut(s) 261
Psp5II RGGWCCY 2 cut(s) 46, 159
PspN4I GGNNCC 3 cut(s) 6, 48, 160
PspPI GGNCC 4 cut(s) 46, 159, 518, 670
PspPPI RGGWCCY 2 cut(s) 46, 159
PsuI RGATCY 2 cut(s) 127, 648
PsyI GACNNNGTC 1 cut(s) 272
SacI GAGCTC 1 cut(s) 261
SacII CCGCGG 2 cut(s) 220, 389
SalI GTCGAC 1 cut(s) 549
Sau3AI GATC 4 cut(s) 127, 445, 584, 648
Sau96I GGNCC 4 cut(s) 46, 159, 518, 670
SchI GAGTC 2 cut(s) 330, 522
ScrFI CCNGG 3 cut(s) 80, 132, 133
SduI GDGCHC 1 cut(s) 261
SetI ASST 8 cut(s) 164, 261, 313, 358, 489, 567, 595, 601
Sfr303I CCGCGG 2 cut(s) 220, 389
SgrBI CCGCGG 2 cut(s) 220, 389
SinI GGWCC 3 cut(s) 46, 159, 670
SmaI CCCGGG 1 cut(s) 133
Sse9I AATT 2 cut(s) 212, 305
SseBI AGGCCT 1 cut(s) 97
SstI GAGCTC 1 cut(s) 261
StuI AGGCCT 1 cut(s) 97
StyD4I CCNGG 3 cut(s) 78, 130, 131
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 1 cut(s) 317
TaqI TCGA 9 cut(s) 165, 267, 352, 454, 490, 550, 556, 583, 589
TaqII GACCGA 1 cut(s) 533
TasI AATT 2 cut(s) 212, 305
TfiI GAWTC 1 cut(s) 75
TscAI CASTG 1 cut(s) 259
TseI GCWGC 1 cut(s) 182
TspDTI ATGAA 1 cut(s) 305
TspGWI ACGGA 1 cut(s) 29
TspMI CCCGGG 1 cut(s) 131
TspRI CASTG 1 cut(s) 259
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 3 cut(s) 46, 159, 670
XapI RAATTY 1 cut(s) 212
XmaI CCCGGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.