pycom12g03740

Belongs to the universal ribosomal protein uL22 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002533
Physical Location & Seq
Reverse (-)
3653111 .. 3655301
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 816 bp
ATGGCGGCTTGGCAGAGGCATCTGCAATCAGCTATCCGTCAAATTGGAAAAAGGGTTGAATTTAGTTACAACCCTCGAGCCAATTTCTGTTCTTCTTCCTCTTCTCGTTTAGAGGGTCTCTTGCCTTATTTCCAAACTCTTTGCAAACCATCTTCTCCAACCATTTCAAGGCCCCTTCATCAATATTTTCAGCACTTGGGAATTTCTAGTTCGAGAAACTTGAGAGCTGAGGAAACACCTCTTCCATCTCCATTGACTCCTGTTTTGGCATCAAATACTGCGATGAATGAGGAGCACAAGCAGAAATCGCTCACTAAACCTGCTAAAGTTCAAGCCATCTTAAAGGGAATAAAGCAGAGCCCTAAGAAGGTCAACTTGGTTGCTGCACTGGTACGTGGTATGCGTGTTGAAGATGCATTGTTGCAGTTGCAAGTGACAGTAAAGCGAGCTTCAAAAACTGTGTACCAGGTCATTCATTCAGCCCGAGCAAATGCAACTCATAATCATGGGTTGGATCCAGACCGTCTCCTTGTTGCCGAGGCTTTTGTTGGAAAGGGATTCTTCAAAAAGAGGCTATCCTACCATGCTAAAGGCAAATGTGGAGTGAAAGTGAGACCAGAATGCCGCCTTACGGTTGTAGTGAGGGAGATTACCCCTGACGAGGAGGCAGAGATAGCCAAACTGAAGGTTAAGAATTTCATTAAGATGACTAAGCGCGAAAGAAAGCTTGTTCCTCACCAACTTATTGAGACAACTCCAATTTGGGGCCGCAAAGGCAGAAGTCGTGATCGTGAACGAAATGGTGAGGCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22

Protein Analysis

272

Amino Acids

30.56

Weight (kDa)

10.41

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 112 - 215 7.3e-29 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 328
AccII CGCG 1 cut(s) 717
AciI CCGC 3 cut(s) 5, 625, 769
AclWI GGATC 2 cut(s) 509, 522
AcsI RAATTY 3 cut(s) 59, 201, 694
AcuI CTGAAG 1 cut(s) 704
AfaI GTAC 2 cut(s) 393, 464
AfiI CCNNNNNNNGG 5 cut(s) 168, 367, 631, 661, 764
AgsI TTSAA 6 cut(s) 59, 168, 332, 410, 453, 565
AjnI CCWGG 1 cut(s) 465
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AluBI AGCT 4 cut(s) 32, 227, 449, 727
AluI AGCT 4 cut(s) 32, 227, 449, 727
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 4 cut(s) 122, 530, 607, 743
AlwI GGATC 2 cut(s) 509, 522
Ama87I CYCGRG 2 cut(s) 75, 483
AoxI GGCC 2 cut(s) 170, 766
ApeKI GCWGC 1 cut(s) 383
ApoI RAATTY 3 cut(s) 59, 201, 694
ArsI GACNNNNNNTTYG 2 cut(s) 247, 279
AspLEI GCGC 1 cut(s) 717
AspS9I GGNCC 2 cut(s) 171, 766
AsuHPI GGTGA 2 cut(s) 728, 815
AvaI CYCGRG 2 cut(s) 75, 483
BamHI GGATCC 1 cut(s) 514
BanII GRGCYC 1 cut(s) 362
Bbv12I GWGCWC 1 cut(s) 297
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 370
BccI CCATC 3 cut(s) 157, 253, 344
BciT130I CCWGG 1 cut(s) 467
BcoDI GTCTC 4 cut(s) 122, 530, 607, 743
BfaI CTAG 1 cut(s) 207
BfuAI ACCTGC 1 cut(s) 328
BglI GCCNNNNNGGC 1 cut(s) 774
BisI GCNGC 4 cut(s) 6, 384, 625, 769
BlsI GCNGC 4 cut(s) 7, 385, 626, 770
Bme1390I CCNGG 1 cut(s) 467
BmeT110I CYCGRG 2 cut(s) 75, 483
BmgT120I GGNCC 2 cut(s) 171, 766
BmiI GGNNCC 3 cut(s) 173, 516, 767
BmrFI CCNGG 1 cut(s) 467
BmsI GCATC 3 cut(s) 28, 278, 403
Bpu10I CCTNAGC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 241
BsaAI YACGTR 1 cut(s) 395
BsaI GGTCTC 2 cut(s) 122, 607
BsaJI CCNNGG 1 cut(s) 537
BsaXI ACNNNNNCTCC 2 cut(s) 594, 624
Bsc4I CCNNNNNNNGG 5 cut(s) 168, 367, 631, 661, 764
Bse1I ACTGG 1 cut(s) 393
BseBI CCWGG 1 cut(s) 467
BseDI CCNNGG 1 cut(s) 537
BseLI CCNNNNNNNGG 5 cut(s) 168, 367, 631, 661, 764
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 393
BseRI GAGGAG 2 cut(s) 305, 677
BseXI GCAGC 1 cut(s) 370
BsgI GTGCAG 1 cut(s) 369
Bsh1236I CGCG 1 cut(s) 717
BshFI GGCC 2 cut(s) 172, 768
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 2 cut(s) 75, 483
BslI CCNNNNNNNGG 5 cut(s) 168, 367, 631, 661, 764
BsmAI GTCTC 4 cut(s) 122, 530, 607, 743
BsmBI CGTCTC 1 cut(s) 530
BsmI GAATGC 1 cut(s) 626
BsnI GGCC 2 cut(s) 172, 768
Bso31I GGTCTC 2 cut(s) 122, 607
BsoBI CYCGRG 2 cut(s) 75, 483
Bsp1286I GDGCHC 2 cut(s) 297, 362
Bsp143I GATC 2 cut(s) 514, 787
BspACI CCGC 3 cut(s) 5, 625, 769
BspANI GGCC 2 cut(s) 172, 768
BspCNI CTCAG 1 cut(s) 220
BspFNI CGCG 1 cut(s) 717
BspHI TCATGA 1 cut(s) 812
BspLI GGNNCC 3 cut(s) 173, 516, 767
BspMI ACCTGC 1 cut(s) 328
BspPI GGATC 2 cut(s) 509, 522
BspTNI GGTCTC 2 cut(s) 122, 607
BsrI ACTGG 1 cut(s) 393
BssECI CCNNGG 1 cut(s) 537
BssMI GATC 2 cut(s) 514, 787
Bst2UI CCWGG 1 cut(s) 467
Bst4CI ACNGT 4 cut(s) 439, 460, 524, 634
Bst6I CTCTTC 2 cut(s) 106, 246
BstBAI YACGTR 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 3 cut(s) 228, 363, 711
BstFNI CGCG 1 cut(s) 717
BstHHI GCGC 1 cut(s) 717
BstKTI GATC 2 cut(s) 517, 790
BstMAI GTCTC 4 cut(s) 122, 530, 607, 743
BstMBI GATC 2 cut(s) 514, 787
BstMWI GCNNNNNNNGC 3 cut(s) 307, 674, 774
BstNI CCWGG 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 465
BstUI CGCG 1 cut(s) 717
BstV1I GCAGC 1 cut(s) 370
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BsuRI GGCC 2 cut(s) 172, 768
BtgZI GCGATG 1 cut(s) 296
BtsIMutI CAGTG 1 cut(s) 386
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 1 cut(s) 447
CciI TCATGA 1 cut(s) 812
CfoI GCGC 1 cut(s) 717
Cfr13I GGNCC 2 cut(s) 171, 766
CsiI ACCWGGT 1 cut(s) 465
Csp6I GTAC 2 cut(s) 392, 463
CviAII CATG 3 cut(s) 506, 584, 813
CviQI GTAC 2 cut(s) 392, 463
DdeI CTNAG 3 cut(s) 228, 363, 711
DpnI GATC 2 cut(s) 516, 789
DpnII GATC 2 cut(s) 514, 787
Eam1104I CTCTTC 2 cut(s) 106, 246
EarI CTCTTC 2 cut(s) 106, 246
Eco24I GRGCYC 1 cut(s) 362
Eco31I GGTCTC 2 cut(s) 122, 607
Eco57I CTGAAG 1 cut(s) 704
Eco88I CYCGRG 2 cut(s) 75, 483
EcoO109I RGGNCCY 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 362
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 3 cut(s) 509, 587, 816
FaiI YATR 5 cut(s) 401, 501, 507, 585, 814
FalI AAGNNNNNCTT 4 cut(s) 359, 391, 545, 577
FatI CATG 3 cut(s) 505, 583, 812
Fnu4HI GCNGC 4 cut(s) 6, 384, 625, 769
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 4 cut(s) 6, 384, 625, 769
FspBI CTAG 1 cut(s) 207
GlaI GCGC 1 cut(s) 716
GluI GCNGC 4 cut(s) 6, 384, 625, 769
HaeIII GGCC 2 cut(s) 172, 768
HhaI GCGC 1 cut(s) 717
Hin1II CATG 3 cut(s) 509, 587, 816
Hin6I GCGC 1 cut(s) 715
HinP1I GCGC 1 cut(s) 715
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 2 cut(s) 256, 558
HphI GGTGA 2 cut(s) 728, 815
Hpy166II GTNNAC 3 cut(s) 373, 463, 794
Hpy188III TCNNGA 5 cut(s) 213, 518, 785, 791, 813
Hpy8I GTNNAC 3 cut(s) 373, 463, 794
HpyAV CCTTC 3 cut(s) 185, 361, 679
HpyCH4III ACNGT 4 cut(s) 439, 460, 524, 634
HpyCH4IV ACGT 1 cut(s) 394
HpyCH4V TGCA 7 cut(s) 25, 144, 386, 416, 424, 430, 494
HpyF10VI GCNNNNNNNGC 3 cut(s) 307, 674, 774
HpyF3I CTNAG 3 cut(s) 228, 363, 711
HpySE526I ACGT 1 cut(s) 394
Hsp92II CATG 3 cut(s) 509, 587, 816
HspAI GCGC 1 cut(s) 715
Kzo9I GATC 2 cut(s) 514, 787
LmnI GCTCC 1 cut(s) 292
LpnPI CCDG 8 cut(s) 273, 333, 374, 452, 479, 531, 630, 669
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 3 cut(s) 28, 278, 403
MabI ACCWGGT 1 cut(s) 465
MaeI CTAG 1 cut(s) 207
MaeII ACGT 1 cut(s) 394
MaeIII GTNAC 2 cut(s) 65, 433
MalI GATC 2 cut(s) 516, 789
MboI GATC 2 cut(s) 514, 787
MboII GAAGA 7 cut(s) 84, 87, 93, 144, 233, 422, 553
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 2 cut(s) 297, 362
MluCI AATT 6 cut(s) 42, 59, 82, 201, 694, 759
MlyI GAGTC 1 cut(s) 250
MmeI TCCRAC 3 cut(s) 182, 492, 529
Mph1103I ATGCAT 1 cut(s) 418
MseI TTAA 3 cut(s) 341, 690, 702
MslI CAYNNNNRTG 2 cut(s) 504, 704
MspR9I CCNGG 1 cut(s) 467
Mva1269I GAATGC 1 cut(s) 626
MvaI CCWGG 1 cut(s) 467
MvnI CGCG 1 cut(s) 717
MwoI GCNNNNNNNGC 3 cut(s) 307, 674, 774
NdeII GATC 2 cut(s) 514, 787
NlaIII CATG 3 cut(s) 509, 587, 816
NlaIV GGNNCC 3 cut(s) 173, 516, 767
NmeAIII GCCGAG 1 cut(s) 562
NmuCI GTSAC 1 cut(s) 433
NsiI ATGCAT 1 cut(s) 418
PaeR7I CTCGAG 1 cut(s) 75
PagI TCATGA 1 cut(s) 812
PcsI WCGNNNNNNNCGW 1 cut(s) 400
PctI GAATGC 1 cut(s) 626
PfeI GAWTC 1 cut(s) 558
PkrI GCNGC 4 cut(s) 7, 385, 626, 770
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
Ppu21I YACGTR 1 cut(s) 395
Psp6I CCWGG 1 cut(s) 465
PspGI CCWGG 1 cut(s) 465
PspN4I GGNNCC 3 cut(s) 173, 516, 767
PspPI GGNCC 2 cut(s) 171, 766
PspXI VCTCGAGB 1 cut(s) 75
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 2 cut(s) 393, 464
RsaNI GTAC 2 cut(s) 392, 463
RseI CAYNNNNRTG 2 cut(s) 504, 704
SaqAI TTAA 3 cut(s) 341, 690, 702
SatI GCNGC 4 cut(s) 6, 384, 625, 769
Sau3AI GATC 2 cut(s) 514, 787
Sau96I GGNCC 2 cut(s) 171, 766
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 467
SduI GDGCHC 2 cut(s) 297, 362
SexAI ACCWGGT 1 cut(s) 465
SfaNI GCATC 3 cut(s) 28, 278, 403
Sfr274I CTCGAG 1 cut(s) 75
SlaI CTCGAG 1 cut(s) 75
SmiMI CAYNNNNRTG 2 cut(s) 504, 704
SmlI CTYRAG 2 cut(s) 75, 220
SmoI CTYRAG 2 cut(s) 75, 220
Sse9I AATT 6 cut(s) 42, 59, 82, 201, 694, 759
SsiI CCGC 3 cut(s) 5, 625, 769
SspI AATATT 1 cut(s) 185
SspMI CTAG 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 465
TaaI ACNGT 4 cut(s) 439, 460, 524, 634
TaiI ACGT 1 cut(s) 397
TaqI TCGA 2 cut(s) 76, 212
TasI AATT 6 cut(s) 42, 59, 82, 201, 694, 759
TauI GCSGC 3 cut(s) 8, 627, 771
TfiI GAWTC 1 cut(s) 558
Tru1I TTAA 3 cut(s) 341, 690, 702
Tru9I TTAA 3 cut(s) 341, 690, 702
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 5 cut(s) 167, 299, 464, 688, 801
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 1 cut(s) 393
XapI RAATTY 3 cut(s) 59, 201, 694
XhoI CTCGAG 1 cut(s) 75
XspI CTAG 1 cut(s) 207
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.