pycom12g04890

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003999
Physical Location & Seq
Reverse (-)
4837872 .. 4841151
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
ATGTCGTCTTCTTCTTCTTCAACTGCTCACTCCTTGGCATTAGCAACCACATATGTAGTAGTTTTACTTCTGCTACATGTACCTAACTATAATGCTCAACTGAACTCTACATTTTACTCCACCACCTGTCCAAACGTGACAAGCATTGTGCGCAGTGCGGTTCAACAAGCTTTGCAATCTGATTCGAGGATTGGTGCAAGCCTTATTCGTCTCCATTTCCATGATTGCTTCGTCAATGGTTGTGATGCTTCAATCTTGTTGGACAAAAATGGAACCATACAACAGAGTGAGAAAGATGCAGCTCCAAACACCAACTCCACTCGAGGCTTTGATGTGGTTGACAATATCAAGACTGCTCTGGAAAATTCATGCCCAGGTGTTGTATCTTGTGCTGACCTTCTTGCGCTTGCGGCTGAAGCTTCTGTTTCTTTGTCAGGAGGCCCTGCATGGAATGTATTATTAGGGAGAAGAGACAGCCTAACAGCAAACCAGGCTGGAGCCAATACCTCTATTCCTTCACCTTTTGAAAGCTTAGCAAACATTACTTCCAAGTTTTCTGCTGTTGGGCTGAACACCAACGACCTCGTTGCATTATCTGGGGCACATACATTTGGACGAGCTCAATGTCGAACATTTAGCAATCGATTGTACAACTTCAACAGCACAGGAAACCCCGATCCAACACTGAACTCATCTTACTTGACCACTCTCCAACAAACATGTCCACAAAATGGGAGCGGAACTGCTCTAGCCAACCTCGATCTCAGCACTCCAGATGCGTTTGACAACAACTACTTCACAAACCTTCAAAACAATCAAGGCCTTCTGCAATCCGATCAAGAGTTATTTTCGACGGCAGGGGCTGCCACAGTTTCCATTGTTAATAGCTTTAGTAGCAACCAGAGCGCCTTCTTTCAGAGCTTTGCTCAGTCGATGATCAATATGGGAAATATCAGTCCACTAGTGGGAACTAGTGGGGAGATTAGGTTGGATTGTAAGAATGTCAATGGAGGTTAA

Protein Analysis

339

Amino Acids

35.58

Weight (kDa)

5.07

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 50 - 299 7.7e-88 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 152
AccB7I CCANNNNNTGG 1 cut(s) 731
AccBSI CCGCTC 1 cut(s) 738
AciI CCGC 3 cut(s) 158, 410, 738
AclWI GGATC 1 cut(s) 671
AcsI RAATTY 1 cut(s) 364
AcuI CTGAAG 1 cut(s) 435
AfaI GTAC 2 cut(s) 81, 650
AfiI CCNNNNNNNGG 2 cut(s) 731, 965
AflIII ACRYGT 2 cut(s) 76, 719
AgsI TTSAA 6 cut(s) 21, 164, 252, 527, 658, 809
AhlI ACTAGT 2 cut(s) 961, 971
AjnI CCWGG 2 cut(s) 373, 489
AluBI AGCT 7 cut(s) 170, 302, 419, 531, 620, 888, 921
AluI AGCT 7 cut(s) 170, 302, 419, 531, 620, 888, 921
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 2 cut(s) 215, 465
AlwI GGATC 1 cut(s) 671
AlwNI CAGNNNCTG 1 cut(s) 863
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 2 cut(s) 439, 820
ApeKI GCWGC 2 cut(s) 299, 863
ApoI RAATTY 1 cut(s) 364
AspLEI GCGC 3 cut(s) 153, 406, 908
AspS9I GGNCC 1 cut(s) 440
AsuHPI GGTGA 1 cut(s) 510
AvaI CYCGRG 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 604
BanII GRGCYC 1 cut(s) 622
BarI GAAGNNNNNNTAC 2 cut(s) 51, 83
Bbv12I GWGCWC 1 cut(s) 622
BbvI GCAGC 2 cut(s) 311, 850
BceAI ACGGC 1 cut(s) 870
BcgI CGANNNNNNTGC 2 cut(s) 569, 603
BciT130I CCWGG 2 cut(s) 375, 491
BclI TGATCA 1 cut(s) 936
BcoDI GTCTC 2 cut(s) 215, 465
BcuI ACTAGT 2 cut(s) 961, 971
BfaI CTAG 3 cut(s) 749, 962, 972
BfoI RGCGCY 1 cut(s) 909
BisI GCNGC 3 cut(s) 300, 411, 864
BlpI GCTNAGC 1 cut(s) 532
BlsI GCNGC 3 cut(s) 301, 412, 865
Bme1390I CCNGG 2 cut(s) 375, 491
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 2 cut(s) 274, 499
BmrFI CCNGG 2 cut(s) 375, 491
BmsI GCATC 3 cut(s) 235, 286, 766
BplI GAGNNNNNCTC 2 cut(s) 910, 942
BpmI CTGGAG 2 cut(s) 516, 756
Bpu1102I GCTNAGC 1 cut(s) 532
Bsa29I ATCGAT 1 cut(s) 643
BsaJI CCNNGG 2 cut(s) 33, 373
BsaXI ACNNNNNCTCC 4 cut(s) 299, 329, 971, 1001
Bsc4I CCNNNNNNNGG 2 cut(s) 731, 965
BseBI CCWGG 2 cut(s) 375, 491
BseCI ATCGAT 1 cut(s) 643
BseDI CCNNGG 2 cut(s) 33, 373
BseLI CCNNNNNNNGG 2 cut(s) 731, 965
BseMII CTCAG 2 cut(s) 778, 941
BseSI GKGCMC 1 cut(s) 604
BseXI GCAGC 2 cut(s) 311, 850
BshFI GGCC 2 cut(s) 441, 822
BshVI ATCGAT 1 cut(s) 643
BsiHKAI GWGCWC 1 cut(s) 622
BsiHKCI CYCGRG 1 cut(s) 321
BslI CCNNNNNNNGG 2 cut(s) 731, 965
BsmAI GTCTC 2 cut(s) 215, 465
BsmBI CGTCTC 1 cut(s) 215
BsnI GGCC 2 cut(s) 441, 822
BsoBI CYCGRG 1 cut(s) 321
Bsp1286I GDGCHC 2 cut(s) 604, 622
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 4 cut(s) 676, 760, 835, 936
Bsp1720I GCTNAGC 1 cut(s) 532
BspACI CCGC 3 cut(s) 158, 410, 738
BspANI GGCC 2 cut(s) 441, 822
BspCNI CTCAG 2 cut(s) 777, 940
BspDI ATCGAT 1 cut(s) 643
BspLI GGNNCC 2 cut(s) 274, 499
BspPI GGATC 1 cut(s) 671
BsrBI CCGCTC 1 cut(s) 738
BsrGI TGTACA 1 cut(s) 648
BssECI CCNNGG 2 cut(s) 33, 373
BssMI GATC 4 cut(s) 676, 760, 835, 936
BssT1I CCWWGG 1 cut(s) 33
Bst2UI CCWGG 2 cut(s) 375, 491
Bst4CI ACNGT 1 cut(s) 871
Bst6I CTCTTC 1 cut(s) 463
BstAPI GCANNNNNTGC 1 cut(s) 863
BstAUI TGTACA 1 cut(s) 648
BstC8I GCNNGC 2 cut(s) 199, 408
BstDEI CTNAG 3 cut(s) 532, 764, 927
BstH2I RGCGCY 1 cut(s) 909
BstHHI GCGC 3 cut(s) 153, 406, 908
BstKTI GATC 4 cut(s) 679, 763, 838, 939
BstMAI GTCTC 2 cut(s) 215, 465
BstMBI GATC 4 cut(s) 676, 760, 835, 936
BstMWI GCNNNNNNNGC 7 cut(s) 150, 410, 416, 491, 863, 894, 903
BstNI CCWGG 2 cut(s) 375, 491
BstNSI RCATGY 2 cut(s) 80, 723
BstSCI CCNGG 2 cut(s) 373, 489
BstSLI GKGCMC 1 cut(s) 604
BstV1I GCAGC 2 cut(s) 311, 850
Bsu15I ATCGAT 1 cut(s) 643
BsuRI GGCC 2 cut(s) 441, 822
BsuTUI ATCGAT 1 cut(s) 643
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 2 cut(s) 160, 683
Cac8I GCNNGC 2 cut(s) 199, 408
CaiI CAGNNNCTG 1 cut(s) 863
CfoI GCGC 3 cut(s) 153, 406, 908
Cfr13I GGNCC 1 cut(s) 440
ClaI ATCGAT 1 cut(s) 643
Csp6I GTAC 2 cut(s) 80, 649
CviAII CATG 5 cut(s) 77, 221, 369, 447, 720
CviQI GTAC 2 cut(s) 80, 649
DdeI CTNAG 3 cut(s) 532, 764, 927
DpnI GATC 4 cut(s) 678, 762, 837, 938
DpnII GATC 4 cut(s) 676, 760, 835, 936
Eam1104I CTCTTC 1 cut(s) 463
EarI CTCTTC 1 cut(s) 463
Ecl136II GAGCTC 1 cut(s) 620
Eco130I CCWWGG 1 cut(s) 33
Eco147I AGGCCT 1 cut(s) 822
Eco24I GRGCYC 1 cut(s) 622
Eco53kI GAGCTC 1 cut(s) 620
Eco57I CTGAAG 1 cut(s) 435
Eco88I CYCGRG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 440
EcoRII CCWGG 2 cut(s) 373, 489
EcoT14I CCWWGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 33
Esp3I CGTCTC 1 cut(s) 215
FaeI CATG 5 cut(s) 80, 224, 372, 450, 723
FatI CATG 5 cut(s) 76, 220, 368, 446, 719
FauNDI CATATG 1 cut(s) 52
FbaI TGATCA 1 cut(s) 936
Fnu4HI GCNGC 3 cut(s) 300, 411, 864
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 3 cut(s) 300, 411, 864
FspBI CTAG 3 cut(s) 749, 962, 972
FspI TGCGCA 1 cut(s) 152
GlaI GCGC 3 cut(s) 152, 405, 907
GluI GCNGC 3 cut(s) 300, 411, 864
GsuI CTGGAG 2 cut(s) 516, 756
HaeII RGCGCY 1 cut(s) 909
HaeIII GGCC 2 cut(s) 441, 822
HhaI GCGC 3 cut(s) 153, 406, 908
Hin1II CATG 5 cut(s) 80, 224, 372, 450, 723
Hin6I GCGC 3 cut(s) 151, 404, 906
HinP1I GCGC 3 cut(s) 151, 404, 906
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 3 cut(s) 168, 417, 529
HinfI GANTC 1 cut(s) 182
HphI GGTGA 1 cut(s) 510
Hpy166II GTNNAC 3 cut(s) 340, 725, 959
Hpy188I TCNGA 3 cut(s) 181, 835, 918
Hpy188III TCNNGA 5 cut(s) 349, 359, 435, 773, 839
Hpy8I GTNNAC 3 cut(s) 340, 725, 959
Hpy99I CGWCG 1 cut(s) 856
HpyAV CCTTC 5 cut(s) 407, 525, 815, 833, 919
HpyCH4III ACNGT 1 cut(s) 871
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 6 cut(s) 175, 197, 299, 446, 590, 829
HpyF10VI GCNNNNNNNGC 7 cut(s) 150, 410, 416, 491, 863, 894, 903
HpyF3I CTNAG 3 cut(s) 532, 764, 927
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 5 cut(s) 80, 224, 372, 450, 723
HspAI GCGC 3 cut(s) 151, 404, 906
Ksp22I TGATCA 1 cut(s) 936
Kzo9I GATC 4 cut(s) 676, 760, 835, 936
LmnI GCTCC 3 cut(s) 307, 497, 735
Lsp1109I GCAGC 2 cut(s) 311, 850
LweI GCATC 3 cut(s) 235, 286, 766
MaeI CTAG 3 cut(s) 749, 962, 972
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 136
MalI GATC 4 cut(s) 678, 762, 837, 938
MbiI CCGCTC 1 cut(s) 738
MboI GATC 4 cut(s) 676, 760, 835, 936
MboII GAAGA 4 cut(s) 3, 6, 9, 480
MhlI GDGCHC 2 cut(s) 604, 622
MluCI AATT 1 cut(s) 364
MmeI TCCRAC 4 cut(s) 240, 704, 736, 969
MnlI CCTC 7 cut(s) 180, 317, 431, 517, 593, 767, 1004
MseI TTAA 2 cut(s) 882, 1015
MslI CAYNNNNRTG 1 cut(s) 219
MspR9I CCNGG 2 cut(s) 375, 491
MvaI CCWGG 2 cut(s) 375, 491
MwoI GCNNNNNNNGC 7 cut(s) 150, 410, 416, 491, 863, 894, 903
NdeI CATATG 1 cut(s) 52
NdeII GATC 4 cut(s) 676, 760, 835, 936
NlaIII CATG 5 cut(s) 80, 224, 372, 450, 723
NlaIV GGNNCC 2 cut(s) 274, 499
NmuCI GTSAC 1 cut(s) 136
NsbI TGCGCA 1 cut(s) 152
NspI RCATGY 2 cut(s) 80, 723
PaeR7I CTCGAG 1 cut(s) 321
PceI AGGCCT 1 cut(s) 822
PciI ACATGT 2 cut(s) 76, 719
PfeI GAWTC 1 cut(s) 182
PflMI CCANNNNNTGG 1 cut(s) 731
PkrI GCNGC 3 cut(s) 301, 412, 865
PscI ACATGT 2 cut(s) 76, 719
Psp124BI GAGCTC 1 cut(s) 622
Psp6I CCWGG 2 cut(s) 373, 489
PspGI CCWGG 2 cut(s) 373, 489
PspN4I GGNNCC 2 cut(s) 274, 499
PspPI GGNCC 1 cut(s) 440
PspXI VCTCGAGB 1 cut(s) 321
PsrI GAACNNNNNNTAC 2 cut(s) 680, 712
PstNI CAGNNNCTG 1 cut(s) 863
RsaI GTAC 2 cut(s) 81, 650
RsaNI GTAC 2 cut(s) 80, 649
RseI CAYNNNNRTG 1 cut(s) 219
SacI GAGCTC 1 cut(s) 622
SaqAI TTAA 2 cut(s) 882, 1015
SatI GCNGC 3 cut(s) 300, 411, 864
Sau3AI GATC 4 cut(s) 676, 760, 835, 936
Sau96I GGNCC 1 cut(s) 440
ScrFI CCNGG 2 cut(s) 375, 491
SduI GDGCHC 2 cut(s) 604, 622
SfaNI GCATC 3 cut(s) 235, 286, 766
Sfr274I CTCGAG 1 cut(s) 321
SlaI CTCGAG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
SpeI ACTAGT 2 cut(s) 961, 971
Sse9I AATT 1 cut(s) 364
SseBI AGGCCT 1 cut(s) 822
SsiI CCGC 3 cut(s) 158, 410, 738
SspMI CTAG 3 cut(s) 749, 962, 972
SstI GAGCTC 1 cut(s) 622
StuI AGGCCT 1 cut(s) 822
StyD4I CCNGG 2 cut(s) 373, 489
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 1 cut(s) 871
TaiI ACGT 1 cut(s) 138
TaqI TCGA 7 cut(s) 185, 322, 628, 643, 759, 851, 932
TasI AATT 1 cut(s) 364
TatI WGTACW 1 cut(s) 648
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 1 cut(s) 182
Tru1I TTAA 2 cut(s) 882, 1015
Tru9I TTAA 2 cut(s) 882, 1015
TscAI CASTG 2 cut(s) 160, 690
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 2 cut(s) 299, 863
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 1 cut(s) 357
TspRI CASTG 2 cut(s) 160, 690
Van91I CCANNNNNTGG 1 cut(s) 731
XapI RAATTY 1 cut(s) 364
XceI RCATGY 2 cut(s) 80, 723
XhoI CTCGAG 1 cut(s) 321
XspI CTAG 3 cut(s) 749, 962, 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.