Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0001873

Physical Location & Seq

Genomic Coordinates Reverse (-)

15207744 .. 15212145

Gene Structure 4402 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3252 bp

ATGGGGAAGGGAGGGCAGGATTTGGGTAGACGGAATGAGGACGGAAACCCAAAGCTGGCCGACGGTGCTGTATTTCCGGCGTGGGCGAAGGAGATTTCGGAGTGCGAGAAGCACTTTGGGGTTAACAGAAAATTTGGGTTGGGATCCAAGGACGTTGAGAAACGGCGGGAGAAGTACGGCTGGAACGAGTTGGAGAAGAACGAGGGGCAGTCGATTTGGAGTCTGGTTCTGGACCAGTTCAATGATACGCTGGTTCGAATCCTGCTTGCGGCGGCGGTCGTTTCTTTCGTTCTGGCTTGGCTGGACGGGGAGGAAGGCAATAAGAAGGAGATCACGGCGTTTGTGGAGCCGCTGGTGATCTTCTTGATCTTGGTTGTGAACGCAATTGTTGGGGTTTGGCAGGAGAGCCATGCCGAGAAGGCGTTGGAGGCGCTGAAGGAGATTCAATCAGAGCAGGCGACTGTGATTCGGGATGGGAGTAAGGTTCCGAATTTGGCGGCCAAGGAGCTTGTTCCTGGTGACATTGTGGAACTTAAGGTTGGGGATAAAGTGTCCGCTGATATGCGTGTTGTGGAATTGATTAGCTCGACTTTGAGGATCGAGCAGGGTTCATTGACTGGAGAGAGTGAGGCAGTGAATAAGACTAACAAGCCAGTGTCTGAGGACGCGGACATTCAGGGGAAGAAAAGTATGGTTTTCGCTGGGACAACTGTTGTGAATGGAAATTGCATATGCTTGGTTGCACAGACAGGAATGTCCACTGAAATCGGGAAGGTTCACATGCAAATCCATGTTGCCTCGCAGAGCGAAGAAGATACCCCACTTAAGAAGAAGCTCAATGAGTTCGGAGAGATGCTCACGATAGTAATTGGAGTTATTTGTGTATTAGTGTGGCTCATCAATTTTAAGTACTTCCTTACTTGGGAATATGTCAATGGACGGCCGGCGAATTTCAAGTTTTCGTTTGAGAAGTGCACATATTACTTTCAGATCGCAGTAGCCTTGGCTGTGGCTGCTATTCCTGAAGGTCTGCCTGCAGTCATTACGACATGCTTGGCGCTTGGTACCCGCAAAATGGCTCAGAAGAATGCCTTGGTCCGAAAGCTGCCTAGTGTTGAAACTCTGGGTTGTACAACTGTGATTTGCTCAGATAAAACAGGGACATTGACTACAAACCAGATGGCAGTGGCAAAACTTGTAGCTTTGGGTCCTAGTCCTACTATACTACGGAAGTGTAGGGTGGATGGAACTACATACAATCCACTTGATGGTAAAATTCACGACTGGCCAACTGGTCGTATGGATGCTAATCTTCAAATGATCGCAAAGGTAGCTGTTGTCTGCAATGATGCTGGCATTACACATGCAGAACAGAAGTACATTGCCAATGGAATGCCTACTGAGGCAGCTCTAAAGATTGAGTATGTTGTTTTGTCACCTCAGGTTTTAGTTGAGAAGATGGGCCTTCCCGAAGAGTCCAAGGGTGCGGAATCAACAAATGAAAGGGATTTGCTTGGTTGTTGCCAGAAGTGGAATGAATTTGAATGCCGAATCGGAACCCTTGAGTTTGATCGTGATAGGAAATCCATGGGTGTAATTGTCAATTCCCGTTCAGGGAAGAAGTCATTGCTAGTGAAGGAATATTGCGGACTTCTGAATGAGGTGCTGTGTTATTGCAGGGCGCTGTTGAGAATTTGCTGGAGAGAAGCACTAAGGTTCAATTGCTTGATGGCACTGTTGTACCACTGGATGATAGTTCAAGGAACTATATTGTGGAAGCTCTTCATGAGATGTCAACTATTGCATTACGCTGTTTGGGTTTTGCATACAAAGATGAGCTCGGTGAGTTTGAATCCTACGATGGTGATGAAGATCGATCCAGCTCATAGGCTTTTACTCGATCCATCCAATTATTCATCAATTGAGAGCGACCTTGTTTTTGTTGGCCTTGTTGGACTGAGGGATCCCCCAAGAGAGGAGGTTTTTGATGCAATTGAAGACTGTCGAGCGGCTGGAATCCGTGTTATTGTAATCACTGGAGATCACCAGAACACAGCAGAAGCAATATGCCGTGAAATAGGCGTGTTTGGTGCTGATGAAGACATACGTTCAAGAAGCCTTACAGGAAGAGGGTTTATGGGTCTACATGATCAGAAAGCTCATCTTAGACAAGGTGGTGGCCTTCTGTTTTCAAGGGCTGAACCAACGCACAAACAAGAGATTGTGAGGTTACTCAAAAAGGATGGTGAAGTGGTTGCTATGACTGGTGATGGAGTGAATGATGCACCTGCTTTGAAACTTGCTGATATCGGGATTGCAATGGGCATTTCTGGAACTGAGGTTGCAAAGGAAGCTTCTGACATGGTTTTGGCTGATGATAATTTTAGCACAATTGTTGCAGCTGTTGGTGAAGGCAGATCTATTTACAATAACATGAAAGTACATGATCGATCATCCAACATTGGTGAGGTCGCCTCCATATTCTTAACAGCAGCATTAGGCATTCCTGAAGGCCTCATACCTGTTCACCTCTTGTGGGTGAATCTTGTTACCGATGGACCACCTGCAACAGCACTAGGATTTAATCCTCCAGACAAAGACATAATGAAGAAACCCCCACGCAGAAGCGAGGAGTCTTTTATCAGCGCTTGGATATTATTCCGCTATCTGGTTATTGGTATGTATGTTGGACTGGCAACTGTTGGCGTATTCGTCATTTGGTATACACATGGTTCCTTCCTAGGTATTGACCTCAGTGGGGACGGCCACTCCCTTGTGACTTACTCCCAACTTACCAACTGGGGACAGTGCTCATCTTGGACAAATTTCACCGCGTCATCCTTCATAGCCGGAAATCAGGTCATCTCTTTTGATGACCCCTGTGATTATTTCCGTGCAGGCAAAATGAAAGCTATGACACTCTCCCTCTCCGTGTTGGTTGCCATCGAAATGTTCAACTCTGTCAATGCCCTTTCCGAGGACGGAAGCCTGCTGAGCATGCCCCCATGGGTCAACCCCTGGCTCCTTGTGGCCATGTGCGTCTCATTTGGCCTGCACTTCATGATCCTGTACGTGCCATTCTTGGCTCAAGTATTTGGCATCGTACCCCTGAGCTTAAACGAATGGCTGCTGGTTTTGGCCGTTGCCCAACCGGTGATTCTCATTGATGAGGTCCTCAAATTTGTAGGAAGATGGGCAAGCGCTTCTCAAATATCCAGGAGGAGAAAACCATCGAAGCCCAAGACAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0010035 GO:0010038 GO:0010042 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031984 GO:0034220 GO:0042175 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1084

Amino Acids

119.17

Weight (kDa)

6.1

Isoelectric Point (pI)

36.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	28 - 95	7.4e-19	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	148 - 260	8e-23	P-type ATPase actuator domain
Cation_ATPase	PF13246	438 - 561	4.3e-07	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	648 - 769	3e-14	haloacid dehalogenase-like hydrolase
HAD	PF12710	658 - 766	5.6e-06	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	838 - 1062	7.6e-47	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07670 AT1G07670 AT1G07810
fragaria_vesca	FvH4_3g34570 FvH4_3g34570 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760
malus_domestica	MD04G1038900.v1.1 MD04G1116400.v1.1 MD11G1117700.v1.1 MD12G1132100.v1.1 MD13G1142700.v1.1
prunus_persica	Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1
pyrus_communis	pycom03g12390 pycom03g12400 pycom04g10640 pycom07g10780 pycom11g09870 pycom12g12810 pycom16g18850
rosa_chinensis	RchiOBHm_Chr3g0467981 RchiOBHm_Chr5g0062231
rosa_laevigata	RLG00000024462 RLG00000035545
rosa_multiflora	Rmu_co8021382.1_g000001 Rmu_sc0001793.1_g000060 Rmu_ssc0000409.1_g000035
rosa_roxburghii	Rroxscaffold_1G00018460 Rroxscaffold_6G00413240
rosa_rugosa	Rorug03G0090700 Rorug03G0090800 Rorug05G0347300
rosa_samantha	Rh3AG139600 Rh3BG161100 Rh3CG161000 Rh3DG161500 Rh5AG407900 Rh5BG421600 Rh5CG445600 Rh5DG436600
rosa_wichuraiana	Rw3G013200 Rw5G038440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	2296, 2572
AasI	GACNNNNNNGTC	1 cut(s)	754
Acc36I	ACCTGC	2 cut(s)	2296, 2572
Acc65I	GGTACC	1 cut(s)	1064
AccB1I	GGYRCC	1 cut(s)	1064
AccB7I	CCANNNNNTGG	1 cut(s)	1268
AccBSI	CCGCTC	1 cut(s)	2009
AccI	GTMKAC	3 cut(s)	28, 2143, 2723
AccII	CGCG	2 cut(s)	668, 2834
AclWI	GGATC	8 cut(s)	138, 151, 605, 1871, 1895, 1958, 1971, 3058
AcoI	YGGCCR	7 cut(s)	57, 498, 941, 1286, 2764, 3030, 3138
AcsI	RAATTY	8 cut(s)	131, 490, 949, 1275, 1538, 1692, 2824, 3179
AcuI	CTGAAG	3 cut(s)	455, 1044, 2529
AfaI	GTAC	9 cut(s)	176, 911, 1066, 1132, 1379, 1742, 2442, 3071, 3105
AfeI	AGCGCT	2 cut(s)	2647, 3202
AflII	CTTAAG	2 cut(s)	533, 824
AgeI	ACCGGT	1 cut(s)	3151
AjnI	CCWGG	3 cut(s)	514, 3017, 3215
AjuI	GAANNNNNNNTTGG	6 cut(s)	522, 554, 1076, 1108, 3141, 3173
Alw21I	GWGCWC	3 cut(s)	977, 1841, 2813
Alw26I	GTCTC	1 cut(s)	3046
Alw44I	GTGCAC	1 cut(s)	973
AlwI	GGATC	8 cut(s)	138, 151, 605, 1871, 1895, 1958, 1971, 3058
AlwNI	CAGNNNCTG	1 cut(s)	659
Aor51HI	AGCGCT	2 cut(s)	2647, 3202
ApaLI	GTGCAC	1 cut(s)	973
ApeKI	GCWGC	6 cut(s)	1013, 1105, 1406, 2399, 2492, 3127
ApoI	RAATTY	8 cut(s)	131, 490, 949, 1275, 1538, 1692, 2824, 3179
ArsI	GACNNNNNNTTYG	2 cut(s)	1922, 1954
AsiGI	ACCGGT	1 cut(s)	3151
Asp700I	GAANNNNTTC	1 cut(s)	1781
Asp718I	GGTACC	1 cut(s)	1064
AspA2I	CCTAGG	1 cut(s)	2740
AspLEI	GCGC	5 cut(s)	433, 1060, 1684, 2648, 3203
AspS9I	GGNCC	6 cut(s)	232, 1096, 1208, 1462, 2558, 3172
AsuII	TTCGAA	1 cut(s)	256
AvaII	GGWCC	5 cut(s)	232, 1096, 1208, 2558, 3172
AvrII	CCTAGG	1 cut(s)	2740
AxyI	CCTNAGG	1 cut(s)	1440
BaeGI	GKGCMC	1 cut(s)	977
BaeI	ACNNNNGTAYC	4 cut(s)	237, 270, 1724, 1757
BalI	TGGCCA	2 cut(s)	1288, 3032
BamHI	GGATCC	2 cut(s)	143, 1963
BanI	GGYRCC	1 cut(s)	1064
BanII	GRGCYC	1 cut(s)	1841
BarI	GAAGNNNNNNTAC	2 cut(s)	2502, 2534
BbsI	GAAGAC	2 cut(s)	2004, 2106
Bbv12I	GWGCWC	3 cut(s)	977, 1841, 2813
BbvI	GCAGC	6 cut(s)	1000, 1092, 1418, 2411, 2504, 3114
BceAI	ACGGC	7 cut(s)	179, 193, 351, 956, 2055, 2779, 3125
BciT130I	CCWGG	3 cut(s)	516, 3019, 3217
BclI	TGATCA	1 cut(s)	2149
BcoDI	GTCTC	1 cut(s)	3046
BfaI	CTAG	5 cut(s)	1110, 1212, 1631, 2576, 2741
BfmI	CTRYAG	1 cut(s)	1035
BfoI	RGCGCY	5 cut(s)	434, 1061, 1685, 2649, 3204
BfrI	CTTAAG	2 cut(s)	533, 824
BfuAI	ACCTGC	2 cut(s)	2296, 2572
BglI	GCCNNNNNGGC	1 cut(s)	419
BglII	AGATCT	1 cut(s)	2417
BlnI	CCTAGG	1 cut(s)	2740
BlpI	GCTNAGC	1 cut(s)	2993
BmcAI	AGTACT	1 cut(s)	911
Bme1390I	CCNGG	3 cut(s)	516, 3019, 3217
Bme18I	GGWCC	5 cut(s)	232, 1096, 1208, 2558, 3172
BmgT120I	GGNCC	6 cut(s)	232, 1096, 1208, 1462, 2558, 3172
BmiI	GGNNCC	9 cut(s)	145, 348, 486, 1066, 1209, 1558, 1965, 2734, 3023
BmrFI	CCNGG	3 cut(s)	516, 3019, 3217
BmrI	ACTGGG	1 cut(s)	2809
BmsI	GCATC	6 cut(s)	843, 1294, 1339, 1978, 2272, 3108
BmuI	ACTGGG	1 cut(s)	2809
BpiI	GAAGAC	2 cut(s)	2004, 2106
BplI	GAGNNNNNCTC	4 cut(s)	840, 872, 2459, 2491
BpmI	CTGGAG	4 cut(s)	639, 1720, 2058, 2574
Bpu10I	CCTNAGC	1 cut(s)	3110
Bpu1102I	GCTNAGC	1 cut(s)	2993
Bpu14I	TTCGAA	1 cut(s)	256
BpuEI	CTTGAG	2 cut(s)	1583, 3072
Bsa29I	ATCGAT	2 cut(s)	1875, 2449
BsaAI	YACGTR	1 cut(s)	3073
BsaBI	GATNNNNATC	2 cut(s)	1308, 1871
BsaWI	WCCGGW	1 cut(s)	3151
BsaXI	ACNNNNNCTCC	2 cut(s)	2753, 2783
Bse118I	RCCGGY	2 cut(s)	943, 3151
Bse21I	CCTNAGG	1 cut(s)	1440
Bse3DI	GCAATG	4 cut(s)	1351, 1380, 1625, 2325
Bse8I	GATNNNNATC	2 cut(s)	1308, 1871
BseBI	CCWGG	3 cut(s)	516, 3019, 3217
BseCI	ATCGAT	2 cut(s)	1875, 2449
BseGI	GGATG	8 cut(s)	478, 1249, 1309, 1755, 1904, 2248, 2453, 2837
BseJI	GATNNNNATC	2 cut(s)	1308, 1871
BseMI	GCAATG	4 cut(s)	1351, 1380, 1625, 2325
BseRI	GAGGAG	3 cut(s)	1991, 2645, 3235
BseSI	GKGCMC	1 cut(s)	977
BseX3I	CGGCCG	1 cut(s)	941
BseXI	GCAGC	6 cut(s)	1000, 1092, 1418, 2411, 2504, 3114
BseYI	CCCAGC	1 cut(s)	701
BsgI	GTGCAG	2 cut(s)	2916, 3038
Bsh1236I	CGCG	2 cut(s)	668, 2834
Bsh1285I	CGRYCG	2 cut(s)	279, 944
BshNI	GGYRCC	1 cut(s)	1064
BshTI	ACCGGT	1 cut(s)	3151
BshVI	ATCGAT	2 cut(s)	1875, 2449
BsiEI	CGRYCG	2 cut(s)	279, 944
BsiHKAI	GWGCWC	3 cut(s)	977, 1841, 2813
BsiSI	CCGG	4 cut(s)	77, 944, 2850, 3152
BslFI	GGGAC	4 cut(s)	718, 1174, 2774, 2817
BsmAI	GTCTC	1 cut(s)	3046
BsmBI	CGTCTC	1 cut(s)	3046
BsmFI	GGGAC	4 cut(s)	718, 1174, 2774, 2817
BsmI	GAATGC	4 cut(s)	1093, 1398, 1550, 2502
Bsp119I	TTCGAA	1 cut(s)	256
Bsp1286I	GDGCHC	3 cut(s)	977, 1841, 2813
Bsp1407I	TGTACA	1 cut(s)	1130
Bsp1720I	GCTNAGC	1 cut(s)	2993
Bsp19I	CCATGG	2 cut(s)	1587, 3005
BspDI	ATCGAT	2 cut(s)	1875, 2449
BspFNI	CGCG	2 cut(s)	668, 2834
BspHI	TCATGA	2 cut(s)	1785, 3060
BspLI	GGNNCC	9 cut(s)	145, 348, 486, 1066, 1209, 1558, 1965, 2734, 3023
BspMAI	CTGCAG	1 cut(s)	1039
BspMI	ACCTGC	2 cut(s)	2296, 2572
BspPI	GGATC	8 cut(s)	138, 151, 605, 1871, 1895, 1958, 1971, 3058
BspQI	GCTCTTC	1 cut(s)	1787
BspT104I	TTCGAA	1 cut(s)	256
BspT107I	GGYRCC	1 cut(s)	1064
BspTI	CTTAAG	2 cut(s)	533, 824
BsrBI	CCGCTC	1 cut(s)	2009
BsrDI	GCAATG	4 cut(s)	1351, 1380, 1625, 2325
BsrFI	RCCGGY	2 cut(s)	943, 3151
BsrGI	TGTACA	1 cut(s)	1130
BssAI	RCCGGY	2 cut(s)	943, 3151
BssNAI	GTATAC	1 cut(s)	2724
BssT1I	CCWWGG	8 cut(s)	147, 501, 1002, 1092, 1479, 1587, 2740, 3005
Bst1107I	GTATAC	1 cut(s)	2724
Bst2UI	CCWGG	3 cut(s)	516, 3019, 3217
Bst4CI	ACNGT	8 cut(s)	65, 463, 712, 1138, 1737, 2003, 2701, 2808
Bst6I	CTCTTC	3 cut(s)	1467, 1787, 2122
BstAFI	CTTAAG	2 cut(s)	533, 824
BstAUI	TGTACA	1 cut(s)	1130
BstBAI	YACGTR	1 cut(s)	3073
BstBI	TTCGAA	1 cut(s)	256
BstDSI	CCRYGG	2 cut(s)	1587, 3005
BstENI	CCTNNNNNAGG	1 cut(s)	2975
BstF5I	GGATG	8 cut(s)	478, 1249, 1309, 1755, 1904, 2248, 2453, 2837
BstFNI	CGCG	2 cut(s)	668, 2834
BstH2I	RGCGCY	5 cut(s)	434, 1061, 1685, 2649, 3204
BstHHI	GCGC	5 cut(s)	433, 1060, 1684, 2648, 3203
BstMAI	GTCTC	1 cut(s)	3046
BstMCI	CGRYCG	2 cut(s)	279, 944
BstMWI	GCNNNNNNNGC	8 cut(s)	65, 419, 428, 1013, 1331, 2351, 2994, 2998
BstNI	CCWGG	3 cut(s)	516, 3019, 3217
BstNSI	RCATGY	4 cut(s)	784, 1053, 1367, 3001
BstSCI	CCNGG	3 cut(s)	514, 3017, 3215
BstSFI	CTRYAG	1 cut(s)	1035
BstSLI	GKGCMC	1 cut(s)	977
BstUI	CGCG	2 cut(s)	668, 2834
BstV1I	GCAGC	6 cut(s)	1000, 1092, 1418, 2411, 2504, 3114
BstV2I	GAAGAC	2 cut(s)	2004, 2106
BstX2I	RGATCY	3 cut(s)	143, 1963, 2417
BstYI	RGATCY	3 cut(s)	143, 1963, 2417
BstZ17I	GTATAC	1 cut(s)	2724
BstZI	CGGCCG	1 cut(s)	941
Bsu15I	ATCGAT	2 cut(s)	1875, 2449
Bsu36I	CCTNAGG	1 cut(s)	1440
BsuTUI	ATCGAT	2 cut(s)	1875, 2449
BtgI	CCRYGG	2 cut(s)	1587, 3005
BtsCI	GGATG	8 cut(s)	478, 1249, 1309, 1755, 1904, 2248, 2453, 2837
BtsI	GCAGTG	2 cut(s)	639, 1191
BtsIMutI	CAGTG	9 cut(s)	639, 660, 759, 1191, 1733, 1744, 2034, 2761, 2813
BveI	ACCTGC	2 cut(s)	2296, 2572
CaiI	CAGNNNCTG	1 cut(s)	659
CciI	TCATGA	2 cut(s)	1785, 3060
CfoI	GCGC	5 cut(s)	433, 1060, 1684, 2648, 3203
Cfr10I	RCCGGY	2 cut(s)	943, 3151
Cfr13I	GGNCC	6 cut(s)	232, 1096, 1208, 1462, 2558, 3172
ClaI	ATCGAT	2 cut(s)	1875, 2449
CseI	GACGC	3 cut(s)	674, 2823, 3028
Csp6I	GTAC	9 cut(s)	175, 910, 1065, 1131, 1378, 1741, 2441, 3070, 3104
CspAI	ACCGGT	1 cut(s)	3151
CviQI	GTAC	9 cut(s)	175, 910, 1065, 1131, 1378, 1741, 2441, 3070, 3104
DrdI	GACNNNNNNGTC	1 cut(s)	754
DseDI	GACNNNNNNGTC	1 cut(s)	754
EaeI	YGGCCR	7 cut(s)	57, 498, 941, 1286, 2764, 3030, 3138
EagI	CGGCCG	1 cut(s)	941
Eam1104I	CTCTTC	3 cut(s)	1467, 1787, 2122
EarI	CTCTTC	3 cut(s)	1467, 1787, 2122
Ecl136II	GAGCTC	1 cut(s)	1839
EclXI	CGGCCG	1 cut(s)	941
Eco130I	CCWWGG	8 cut(s)	147, 501, 1002, 1092, 1479, 1587, 2740, 3005
Eco147I	AGGCCT	1 cut(s)	2514
Eco24I	GRGCYC	1 cut(s)	1841
Eco32I	GATATC	1 cut(s)	2308
Eco47I	GGWCC	5 cut(s)	232, 1096, 1208, 2558, 3172
Eco47III	AGCGCT	2 cut(s)	2647, 3202
Eco52I	CGGCCG	1 cut(s)	941
Eco53kI	GAGCTC	1 cut(s)	1839
Eco57I	CTGAAG	3 cut(s)	455, 1044, 2529
Eco81I	CCTNAGG	1 cut(s)	1440
EcoICRI	GAGCTC	1 cut(s)	1839
EcoNI	CCTNNNNNAGG	1 cut(s)	2975
EcoO109I	RGGNCCY	2 cut(s)	1208, 3172
EcoRII	CCWGG	3 cut(s)	514, 3017, 3215
EcoRV	GATATC	1 cut(s)	2308
EcoT14I	CCWWGG	8 cut(s)	147, 501, 1002, 1092, 1479, 1587, 2740, 3005
EcoT38I	GRGCYC	1 cut(s)	1841
ErhI	CCWWGG	8 cut(s)	147, 501, 1002, 1092, 1479, 1587, 2740, 3005
Esp3I	CGTCTC	1 cut(s)	3046
FalI	AAGNNNNNCTT	4 cut(s)	1076, 1108, 2148, 2180
FaqI	GGGAC	4 cut(s)	718, 1174, 2774, 2817
FauI	CCCGC	2 cut(s)	159, 1076
FauNDI	CATATG	1 cut(s)	731
FbaI	TGATCA	1 cut(s)	2149
FblI	GTMKAC	3 cut(s)	28, 2143, 2723
FokI	GGATG	8 cut(s)	485, 1256, 1316, 1762, 1891, 2255, 2440, 2824
FriOI	GRGCYC	1 cut(s)	1841
FspBI	CTAG	5 cut(s)	1110, 1212, 1631, 2576, 2741
GlaI	GCGC	5 cut(s)	432, 1059, 1683, 2647, 3202
GsaI	CCCAGC	1 cut(s)	705
GsuI	CTGGAG	4 cut(s)	639, 1720, 2058, 2574
HaeII	RGCGCY	5 cut(s)	434, 1061, 1685, 2649, 3204
HapII	CCGG	4 cut(s)	77, 944, 2850, 3152
HgaI	GACGC	3 cut(s)	674, 2823, 3028
HhaI	GCGC	5 cut(s)	433, 1060, 1684, 2648, 3203
Hin6I	GCGC	5 cut(s)	431, 1058, 1682, 2646, 3201
HinP1I	GCGC	5 cut(s)	431, 1058, 1682, 2646, 3201
HincII	GTYRAC	3 cut(s)	124, 1796, 3013
HindII	GTYRAC	3 cut(s)	124, 1796, 3013
HindIII	AAGCTT	1 cut(s)	2352
HpaI	GTTAAC	1 cut(s)	124
HpaII	CCGG	4 cut(s)	77, 944, 2850, 3152
Hpy99I	CGWCG	1 cut(s)	65
HpyCH4III	ACNGT	8 cut(s)	65, 463, 712, 1138, 1737, 2003, 2701, 2808
HpyCH4IV	ACGT	3 cut(s)	153, 2107, 3072
HpyF10VI	GCNNNNNNNGC	8 cut(s)	65, 419, 428, 1013, 1331, 2351, 2994, 2998
HpySE526I	ACGT	3 cut(s)	153, 2107, 3072
HspAI	GCGC	5 cut(s)	431, 1058, 1682, 2646, 3201
KpnI	GGTACC	1 cut(s)	1068
KroI	GCCGGC	1 cut(s)	943
KroNI	GCCGGC	1 cut(s)	945
Ksp22I	TGATCA	1 cut(s)	2149
KspAI	GTTAAC	1 cut(s)	124
LguI	GCTCTTC	1 cut(s)	1787
LmnI	GCTCC	3 cut(s)	346, 505, 3027
Lsp1109I	GCAGC	6 cut(s)	1000, 1092, 1418, 2411, 2504, 3114
LweI	GCATC	6 cut(s)	843, 1294, 1339, 1978, 2272, 3108
MaeI	CTAG	5 cut(s)	1110, 1212, 1631, 2576, 2741
MaeII	ACGT	3 cut(s)	153, 2107, 3072
MaeIII	GTNAC	5 cut(s)	518, 1434, 2229, 2548, 2776
MbiI	CCGCTC	1 cut(s)	2009
MfeI	CAATTG	5 cut(s)	384, 1720, 1920, 1992, 2391
MflI	RGATCY	3 cut(s)	143, 1963, 2417
MhlI	GDGCHC	3 cut(s)	977, 1841, 2813
MlsI	TGGCCA	2 cut(s)	1288, 3032
MluNI	TGGCCA	2 cut(s)	1288, 3032
MlyI	GAGTC	3 cut(s)	229, 1484, 2642
MmeI	TCCRAC	5 cut(s)	171, 405, 1933, 2481, 2668
Mox20I	TGGCCA	2 cut(s)	1288, 3032
MroNI	GCCGGC	1 cut(s)	943
MroXI	GAANNNNTTC	1 cut(s)	1781
MscI	TGGCCA	2 cut(s)	1288, 3032
MseI	TTAA	7 cut(s)	123, 534, 825, 906, 2486, 2583, 3116
MslI	CAYNNNNRTG	1 cut(s)	2948
Msp20I	TGGCCA	2 cut(s)	1288, 3032
MspA1I	CMGCKG	3 cut(s)	352, 557, 2402
MspCI	CTTAAG	2 cut(s)	533, 824
MspI	CCGG	4 cut(s)	77, 944, 2850, 3152
MspR9I	CCNGG	3 cut(s)	516, 3019, 3217
MunI	CAATTG	5 cut(s)	384, 1720, 1920, 1992, 2391
Mva1269I	GAATGC	4 cut(s)	1093, 1398, 1550, 2502
MvaI	CCWGG	3 cut(s)	516, 3019, 3217
MvnI	CGCG	2 cut(s)	668, 2834
MwoI	GCNNNNNNNGC	8 cut(s)	65, 419, 428, 1013, 1331, 2351, 2994, 2998
NaeI	GCCGGC	1 cut(s)	945
NcoI	CCATGG	2 cut(s)	1587, 3005
NdeI	CATATG	1 cut(s)	731
NgoMIV	GCCGGC	1 cut(s)	943
NlaIV	GGNNCC	9 cut(s)	145, 348, 486, 1066, 1209, 1558, 1965, 2734, 3023
NmeAIII	GCCGAG	1 cut(s)	439
NmuCI	GTSAC	3 cut(s)	518, 1434, 2776
NspI	RCATGY	4 cut(s)	784, 1053, 1367, 3001
NspV	TTCGAA	1 cut(s)	256
PaeI	GCATGC	1 cut(s)	3001
PagI	TCATGA	2 cut(s)	1785, 3060
PaqCI	CACCTGC	2 cut(s)	2296, 2572
PceI	AGGCCT	1 cut(s)	2514
PciSI	GCTCTTC	1 cut(s)	1787
PcsI	WCGNNNNNNNCGW	2 cut(s)	183, 285
PctI	GAATGC	4 cut(s)	1093, 1398, 1550, 2502
PdiI	GCCGGC	1 cut(s)	945
PdmI	GAANNNNTTC	1 cut(s)	1781
PfeI	GAWTC	9 cut(s)	258, 442, 466, 1490, 1551, 1852, 2016, 2542, 3157
PflMI	CCANNNNNTGG	1 cut(s)	1268
PfoI	TCCNGGA	1 cut(s)	3215
PinAI	ACCGGT	1 cut(s)	3151
PleI	GAGTC	3 cut(s)	228, 1483, 2641
PpsI	GAGTC	3 cut(s)	228, 1483, 2641
Ppu21I	YACGTR	1 cut(s)	3073
PpuMI	RGGWCCY	2 cut(s)	1208, 3172
Psp124BI	GAGCTC	1 cut(s)	1841
Psp5II	RGGWCCY	2 cut(s)	1208, 3172
Psp6I	CCWGG	3 cut(s)	514, 3017, 3215
PspFI	CCCAGC	1 cut(s)	701
PspGI	CCWGG	3 cut(s)	514, 3017, 3215
PspN4I	GGNNCC	9 cut(s)	145, 348, 486, 1066, 1209, 1558, 1965, 2734, 3023
PspPI	GGNCC	6 cut(s)	232, 1096, 1208, 1462, 2558, 3172
PspPPI	RGGWCCY	2 cut(s)	1208, 3172
PstI	CTGCAG	1 cut(s)	1039
PstNI	CAGNNNCTG	1 cut(s)	659
PsuI	RGATCY	3 cut(s)	143, 1963, 2417
PvuII	CAGCTG	1 cut(s)	2402
RsaI	GTAC	9 cut(s)	176, 911, 1066, 1132, 1379, 1742, 2442, 3071, 3105
RsaNI	GTAC	9 cut(s)	175, 910, 1065, 1131, 1378, 1741, 2441, 3070, 3104
RseI	CAYNNNNRTG	1 cut(s)	2948
SacI	GAGCTC	1 cut(s)	1841
SapI	GCTCTTC	1 cut(s)	1787
SaqAI	TTAA	7 cut(s)	123, 534, 825, 906, 2486, 2583, 3116
Sau96I	GGNCC	6 cut(s)	232, 1096, 1208, 1462, 2558, 3172
ScaI	AGTACT	1 cut(s)	911
SchI	GAGTC	3 cut(s)	229, 1484, 2642
ScrFI	CCNGG	3 cut(s)	516, 3019, 3217
SduI	GDGCHC	3 cut(s)	977, 1841, 2813
SfaNI	GCATC	6 cut(s)	843, 1294, 1339, 1978, 2272, 3108
SfcI	CTRYAG	1 cut(s)	1035
SfuI	TTCGAA	1 cut(s)	256
SinI	GGWCC	5 cut(s)	232, 1096, 1208, 2558, 3172
SmiMI	CAYNNNNRTG	1 cut(s)	2948
SmlI	CTYRAG	4 cut(s)	533, 824, 1562, 3087
SmoI	CTYRAG	4 cut(s)	533, 824, 1562, 3087
SphI	GCATGC	1 cut(s)	3001
SseBI	AGGCCT	1 cut(s)	2514
SspI	AATATT	1 cut(s)	1643
SspMI	CTAG	5 cut(s)	1110, 1212, 1631, 2576, 2741
SstI	GAGCTC	1 cut(s)	1841
StuI	AGGCCT	1 cut(s)	2514
StyD4I	CCNGG	3 cut(s)	514, 3017, 3215
StyI	CCWWGG	8 cut(s)	147, 501, 1002, 1092, 1479, 1587, 2740, 3005
TaaI	ACNGT	8 cut(s)	65, 463, 712, 1138, 1737, 2003, 2701, 2808
TaiI	ACGT	3 cut(s)	156, 2110, 3075
TatI	WGTACW	4 cut(s)	909, 1130, 1377, 2440
TauI	GCSGC	5 cut(s)	272, 275, 352, 500, 2012
TfiI	GAWTC	9 cut(s)	258, 442, 466, 1490, 1551, 1852, 2016, 2542, 3157
Tru1I	TTAA	7 cut(s)	123, 534, 825, 906, 2486, 2583, 3116
Tru9I	TTAA	7 cut(s)	123, 534, 825, 906, 2486, 2583, 3116
TscAI	CASTG	9 cut(s)	639, 660, 766, 1191, 1740, 1751, 2041, 2761, 2813
TseFI	GTSAC	3 cut(s)	518, 1434, 2776
TseI	GCWGC	6 cut(s)	1013, 1105, 1406, 2399, 2492, 3127
Tsp45I	GTSAC	3 cut(s)	518, 1434, 2776
TspGWI	ACGGA	7 cut(s)	46, 57, 1243, 2009, 2882, 2920, 2997
TspRI	CASTG	9 cut(s)	639, 660, 766, 1191, 1740, 1751, 2041, 2761, 2813
Van91I	CCANNNNNTGG	1 cut(s)	1268
Vha464I	CTTAAG	2 cut(s)	533, 824
VneI	GTGCAC	1 cut(s)	973
VpaK11BI	GGWCC	5 cut(s)	232, 1096, 1208, 2558, 3172
XagI	CCTNNNNNAGG	1 cut(s)	2975
XapI	RAATTY	8 cut(s)	131, 490, 949, 1275, 1538, 1692, 2824, 3179
XceI	RCATGY	4 cut(s)	784, 1053, 1367, 3001
XmaJI	CCTAGG	1 cut(s)	2740
XmiI	GTMKAC	3 cut(s)	28, 2143, 2723
XmnI	GAANNNNTTC	1 cut(s)	1781
XspI	CTAG	5 cut(s)	1110, 1212, 1631, 2576, 2741
ZrmI	AGTACT	1 cut(s)	911

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.