Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0001521

Physical Location & Seq

Genomic Coordinates Forward (+)

18296917 .. 18300902

Gene Structure 3986 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGACTAGTATACGAGGTATTAATAACTTAAAAGATAAAATTTCTCATCACTTACATAAAAACACGTGGTGTACCATTCGTGTTTCCGTCACAATAAAAAATTTCTTCCGGATCAAGTTAGCAAGTAAAGTACCATTGGTCCATTTGGCGCCAGCTGATTGTCCAACGGAGAAGAAGCGACTGCTTGGTGAGCGGATCGGTTACTCACCCGACCATCCTCCCCTCCGTGGACCTTCTTCCTCAGACATCTACAGGGAAGAACATCCATCGTCTTCCTCCTCCTCCTCATCTTCTTCTTCTTCTGCTGCCGCTTCGACCAGTAGGGACACTTCTGGCGGTGCTGCTGCTTCGACCAGTAGGGACACTTCGGGCGGCGCTGCTGCTGCCGCTACGACTTCGTCTTCGAGCGCTTCGCAGGTGCGGGAGGAGGAGGACGATGACAATCAGTACCAGCAACACACGCATTTCCGGCATCCGGAGCTGGAGCAAAACCACGAAATCGGCACTTCGTACGGCGGAAATGTGCCGGCGTTCGATGAGGTGTCAACGGTGATTACGGATGAATCGTGGTCCTGCATTGTCGTCCTCCTCACCTTCTGGTTCTTCGTGTCGATGACTTTGATTTTGGGAGTTTACGGGTCTTCGAGTTTAGAGCTTGGCCCTAATAGCTCAATTCTTCTGCAACCTAACCCATTTTTTGTGCAGTCTATAAAGGTAGAAGAGCTGAATATATCAAGTTCTGGGCCTGTACTATATGGTTTCTACAGTACTCCACCTCTTGATACTGCTACCACTTGGTCCGAAACACATACTGTCTCGGTTCCAGCCGATTCTCATACGGAATGGATATATTACCTAAATGCGGGGTCTCAAATGAGTATTTCATACAGCGTGAACTCTCCGAGCTTCTCTATTTTTCTTATTATTGCTCAAGGTAAAGAAGGCCTTGCTCAGTGGCTCGAAGAACCGACATATCCTAATACTTCTTTATCATGGAACCTCATTCATGGAAGTGGTATGATTGAGCAGGACCTATCAAAGTCTTCTGCCTACTATGTTTCTGTGGGTAACTTGAACGCAGAGGATGTGGAGGTACAATTAAACATCACAGTGAATGCTTTATTGTACAATACAAATTCGGCTTATTACAAATGTACTTTCACGGATGAATCCTGTACTTTGAAAATTTTGTTTCCGGAGGGAAATGCAGCTGTGTTGACCACTCCTGGTCCAGAGCAGGATACACCTTCTGCAAACCAGTATGTCAAACTATCATATGGACCAAGATGGGTCACATATATTGTTGGTATAGGTGGGATGACTGTGCTCATGTTCTTGGCCTTCAACTTCTTAAACAAATGCCAAGGTACCAATGAAGATGGAATAAGAGTTCGACATGATGAGTTCAGATCTGAGAGAGCCCCATTGCTTTCCTACAAAGATGATGATCGATCAAGTTGGGGCTCATCTTATGATTCTGCCTCAAATGATGAGATCGATCTTGAAGACTTTCTGGCAGGAGAAGGGAAGTCGTCAAGAGATGGTGAAAGTAATAATACTCGTCGTCTTTGTGCAATCTGCTTTGATGCTCCTAGGGACTGCTTTTTCCTTCCCTGCGGGCATTGTGTGGCTTGTTTTGAGTGTGCAACAAGGATAGCAGAGGCAGCTGCTACTTGTCCAATTTGTCGGAGGAACATAAAGAAGGTGAGGAAGATCTTTACAGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0032446 GO:0032991 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4792 DUF4793 zf-C3HC4_3

Protein Analysis

576

Amino Acids

63.08

Weight (kDa)

5.26

Isoelectric Point (pI)

48.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
APD1-4_N	PF16040	248 - 316	1.4e-23	E3 ubiquitin-protein ligase APD1-4, N-terminal
APD1-4_M	PF16041	341 - 446	4.6e-32	E3 ubiquitin-protein ligase APD1-4, middle domain
zf-C3HC4_3	PF13920	521 - 569	6e-14	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	523 - 563	3e-06	Ring finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001521)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01450
fragaria_vesca	FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_3g38600 FvH4_6g11430 FvH4_6g11430 FvH4_6g11430 FvH4_6g11430 FvH4_6g11430
malus_domestica	MD03G1070000.v1.1 MD04G1153100.v1.1 MD11G1073800.v1.1 MD12G1166600.v1.1
prunus_persica	Prupe.6G057100_v2.0.a1 Prupe.6G057100_v2.0.a1 Prupe.6G277400_v2.0.a1 Prupe.6G277400_v2.0.a1
pyrus_communis	pycom03g05570 pycom11g06120 pycom12g15850
rosa_chinensis	RchiOBHm_Chr3g0463121 RchiOBHm_Chr5g0069661
rosa_laevigata	RLG00000024811 RLG00000036082
rosa_multiflora	Rmu_sc0000738.1_g000037 Rmu_sc0000766.1_g000040 Rmu_sc0008595.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011420 Rroxscaffold_6G00416950
rosa_rugosa	Rorug03G0061500 Rorug03G0061600 Rorug03G0061700 Rorug05G0399000 Rorug05G0399100
rosa_samantha	Rh3AG119800 Rh3BG124200 Rh3CG126200 Rh3DG125200 Rh5AG455800 Rh5BG474800 Rh5CG498200 Rh5DG486300
rosa_wichuraiana	Rw3G010130 Rw5G042530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	406
Acc36I	ACCTGC	1 cut(s)	406
Acc65I	GGTACC	1 cut(s)	1367
AccB1I	GGYRCC	2 cut(s)	148, 1367
AccBSI	CCGCTC	1 cut(s)	193
AccI	GTMKAC	1 cut(s)	10
AccIII	TCCGGA	3 cut(s)	108, 475, 1195
AciI	CCGC	9 cut(s)	193, 309, 336, 372, 387, 421, 516, 863, 1617
AclWI	GGATC	2 cut(s)	119, 203
AcsI	RAATTY	4 cut(s)	39, 100, 1135, 1185
AcvI	CACGTG	1 cut(s)	66
AcyI	GRCGYC	1 cut(s)	149
AdeI	CACNNNGTG	1 cut(s)	69
AfeI	AGCGCT	1 cut(s)	409
AfiI	CCNNNNNNNGG	3 cut(s)	227, 475, 862
AflIII	ACRYGT	1 cut(s)	63
AgsI	TTSAA	4 cut(s)	1075, 1183, 1345, 1505
AhlI	ACTAGT	1 cut(s)	5
AjnI	CCWGG	1 cut(s)	1225
AloI	GAACNNNNNNTCC	2 cut(s)	1374, 1406
AluBI	AGCT	8 cut(s)	155, 481, 655, 669, 724, 906, 1211, 1667
AluI	AGCT	8 cut(s)	155, 481, 655, 669, 724, 906, 1211, 1667
Alw21I	GWGCWC	1 cut(s)	1329
Alw26I	GTCTC	2 cut(s)	820, 873
AlwI	GGATC	2 cut(s)	119, 203
Aor13HI	TCCGGA	3 cut(s)	108, 475, 1195
Aor51HI	AGCGCT	1 cut(s)	409
AoxI	GGCC	4 cut(s)	658, 743, 943, 1338
ApeKI	GCWGC	9 cut(s)	305, 341, 344, 377, 380, 383, 1208, 1664, 1667
ApoI	RAATTY	4 cut(s)	39, 100, 1135, 1185
ArsI	GACNNNNNNTTYG	4 cut(s)	385, 417, 607, 639
AseI	ATTAAT	1 cut(s)	21
Asp700I	GAANNNNTTC	1 cut(s)	1509
Asp718I	GGTACC	1 cut(s)	1367
AspA2I	CCTAGG	1 cut(s)	1592
AspLEI	GCGC	3 cut(s)	151, 377, 410
AspS9I	GGNCC	9 cut(s)	139, 230, 570, 659, 743, 798, 1030, 1229, 1280
AsuHPI	GGTGA	6 cut(s)	198, 200, 562, 583, 1556, 1717
AvaII	GGWCC	7 cut(s)	139, 230, 570, 798, 1030, 1229, 1280
AvrII	CCTAGG	1 cut(s)	1592
BanI	GGYRCC	2 cut(s)	148, 1367
BanII	GRGCYC	2 cut(s)	1423, 1466
BarI	GAAGNNNNNNTAC	4 cut(s)	313, 345, 349, 381
BbrPI	CACGTG	1 cut(s)	66
BbsI	GAAGAC	5 cut(s)	264, 393, 633, 1035, 1512
Bbv12I	GWGCWC	1 cut(s)	1329
BbvI	GCAGC	9 cut(s)	292, 328, 331, 364, 367, 370, 1220, 1654, 1676
BccI	CCATC	5 cut(s)	222, 274, 1281, 1373, 1535
BceAI	ACGGC	1 cut(s)	529
BciT130I	CCWGG	1 cut(s)	1227
BciVI	GTATCC	1 cut(s)	1234
BcoDI	GTCTC	2 cut(s)	820, 873
BcuI	ACTAGT	1 cut(s)	5
BfaI	CTAG	2 cut(s)	6, 1593
BfmI	CTRYAG	2 cut(s)	250, 763
BfoI	RGCGCY	3 cut(s)	152, 378, 411
BfuAI	ACCTGC	1 cut(s)	406
BfuI	GTATCC	1 cut(s)	1234
BglII	AGATCT	2 cut(s)	1409, 1713
BlnI	CCTAGG	1 cut(s)	1592
BmcAI	AGTACT	1 cut(s)	769
Bme1390I	CCNGG	1 cut(s)	1227
Bme18I	GGWCC	7 cut(s)	139, 230, 570, 798, 1030, 1229, 1280
BmgT120I	GGNCC	9 cut(s)	139, 230, 570, 659, 743, 798, 1030, 1229, 1280
BmiI	GGNNCC	4 cut(s)	150, 822, 998, 1369
BmrFI	CCNGG	1 cut(s)	1227
BmsI	GCATC	2 cut(s)	481, 1576
BpiI	GAAGAC	5 cut(s)	264, 393, 633, 1035, 1512
BpmI	CTGGAG	1 cut(s)	503
BpuEI	CTTGAG	1 cut(s)	915
Bsa29I	ATCGAT	2 cut(s)	1450, 1497
BsaAI	YACGTR	1 cut(s)	66
BsaBI	GATNNNNATC	2 cut(s)	441, 1446
BsaHI	GRCGYC	1 cut(s)	149
BsaI	GGTCTC	1 cut(s)	873
BsaJI	CCNNGG	3 cut(s)	226, 1363, 1592
BsaWI	WCCGGW	3 cut(s)	108, 475, 1195
Bsc4I	CCNNNNNNNGG	3 cut(s)	227, 475, 862
Bse118I	RCCGGY	1 cut(s)	526
Bse1I	ACTGG	3 cut(s)	318, 354, 1258
Bse3DI	GCAATG	1 cut(s)	1424
Bse8I	GATNNNNATC	2 cut(s)	441, 1446
BseAI	TCCGGA	3 cut(s)	108, 475, 1195
BseBI	CCWGG	1 cut(s)	1227
BseCI	ATCGAT	2 cut(s)	1450, 1497
BseDI	CCNNGG	3 cut(s)	226, 1363, 1592
BseGI	GGATG	7 cut(s)	214, 262, 472, 565, 1090, 1171, 1323
BseJI	GATNNNNATC	2 cut(s)	441, 1446
BseLI	CCNNNNNNNGG	3 cut(s)	227, 475, 862
BseMI	GCAATG	1 cut(s)	1424
BseMII	CTCAG	3 cut(s)	255, 965, 1404
BseNI	ACTGG	3 cut(s)	318, 354, 1258
BseRI	GAGGAG	6 cut(s)	268, 271, 274, 440, 443, 578
BseXI	GCAGC	9 cut(s)	292, 328, 331, 364, 367, 370, 1220, 1654, 1676
BsgI	GTGCAG	1 cut(s)	722
BshFI	GGCC	4 cut(s)	660, 745, 945, 1340
BshNI	GGYRCC	2 cut(s)	148, 1367
BshVI	ATCGAT	2 cut(s)	1450, 1497
BsiHKAI	GWGCWC	1 cut(s)	1329
BsiSI	CCGG	5 cut(s)	109, 469, 476, 527, 1196
BsiWI	CGTACG	1 cut(s)	510
BslFI	GGGAC	3 cut(s)	338, 374, 1610
BslI	CCNNNNNNNGG	3 cut(s)	227, 475, 862
BsmAI	GTCTC	2 cut(s)	820, 873
BsmFI	GGGAC	3 cut(s)	338, 374, 1610
BsmI	GAATGC	1 cut(s)	1120
BsnI	GGCC	4 cut(s)	660, 745, 945, 1340
Bso31I	GGTCTC	1 cut(s)	873
Bsp1286I	GDGCHC	3 cut(s)	1329, 1423, 1466
Bsp13I	TCCGGA	3 cut(s)	108, 475, 1195
Bsp1407I	TGTACA	1 cut(s)	1125
Bsp143I	GATC	8 cut(s)	111, 195, 1409, 1447, 1451, 1494, 1498, 1713
BspACI	CCGC	9 cut(s)	193, 309, 336, 372, 387, 421, 516, 863, 1617
BspANI	GGCC	4 cut(s)	660, 745, 945, 1340
BspCNI	CTCAG	3 cut(s)	254, 964, 1405
BspDI	ATCGAT	2 cut(s)	1450, 1497
BspEI	TCCGGA	3 cut(s)	108, 475, 1195
BspLI	GGNNCC	4 cut(s)	150, 822, 998, 1369
BspMI	ACCTGC	1 cut(s)	406
BspPI	GGATC	2 cut(s)	119, 203
BspQI	GCTCTTC	1 cut(s)	714
BspT107I	GGYRCC	2 cut(s)	148, 1367
BspTNI	GGTCTC	1 cut(s)	873
BsrBI	CCGCTC	1 cut(s)	193
BsrDI	GCAATG	1 cut(s)	1424
BsrFI	RCCGGY	1 cut(s)	526
BsrGI	TGTACA	1 cut(s)	1125
BsrI	ACTGG	3 cut(s)	318, 354, 1258
BssAI	RCCGGY	1 cut(s)	526
BssECI	CCNNGG	3 cut(s)	226, 1363, 1592
BssMI	GATC	8 cut(s)	111, 195, 1409, 1447, 1451, 1494, 1498, 1713
BssNAI	GTATAC	1 cut(s)	11
BssNI	GRCGYC	1 cut(s)	149
BssT1I	CCWWGG	2 cut(s)	1363, 1592
Bst1107I	GTATAC	1 cut(s)	11
Bst2UI	CCWGG	1 cut(s)	1227
Bst4CI	ACNGT	6 cut(s)	550, 767, 814, 1111, 1324, 1723
Bst6I	CTCTTC	1 cut(s)	714
BstACI	GRCGYC	1 cut(s)	149
BstAUI	TGTACA	1 cut(s)	1125
BstBAI	YACGTR	1 cut(s)	66
BstC8I	GCNNGC	3 cut(s)	153, 528, 1619
BstDEI	CTNAG	3 cut(s)	241, 951, 1413
BstDSI	CCRYGG	1 cut(s)	226
BstF5I	GGATG	7 cut(s)	214, 262, 472, 565, 1090, 1171, 1323
BstH2I	RGCGCY	3 cut(s)	152, 378, 411
BstHHI	GCGC	3 cut(s)	151, 377, 410
BstKTI	GATC	8 cut(s)	114, 198, 1412, 1450, 1454, 1497, 1501, 1716
BstMAI	GTCTC	2 cut(s)	820, 873
BstMBI	GATC	8 cut(s)	111, 195, 1409, 1447, 1451, 1494, 1498, 1713
BstMWI	GCNNNNNNNGC	8 cut(s)	190, 383, 386, 460, 469, 478, 666, 1664
BstNI	CCWGG	1 cut(s)	1227
BstSCI	CCNGG	1 cut(s)	1225
BstSFI	CTRYAG	2 cut(s)	250, 763
BstV1I	GCAGC	9 cut(s)	292, 328, 331, 364, 367, 370, 1220, 1654, 1676
BstV2I	GAAGAC	5 cut(s)	264, 393, 633, 1035, 1512
BstX2I	RGATCY	2 cut(s)	1409, 1713
BstYI	RGATCY	2 cut(s)	1409, 1713
BstZ17I	GTATAC	1 cut(s)	11
Bsu15I	ATCGAT	2 cut(s)	1450, 1497
BsuI	GTATCC	1 cut(s)	1234
BsuRI	GGCC	4 cut(s)	660, 745, 945, 1340
BsuTUI	ATCGAT	2 cut(s)	1450, 1497
BtgI	CCRYGG	1 cut(s)	226
BtsCI	GGATG	7 cut(s)	214, 262, 472, 565, 1090, 1171, 1323
BtsIMutI	CAGTG	2 cut(s)	959, 1116
BveI	ACCTGC	1 cut(s)	406
Cac8I	GCNNGC	3 cut(s)	153, 528, 1619
CfoI	GCGC	3 cut(s)	151, 377, 410
Cfr10I	RCCGGY	1 cut(s)	526
Cfr13I	GGNCC	9 cut(s)	139, 230, 570, 659, 743, 798, 1030, 1229, 1280
ClaI	ATCGAT	2 cut(s)	1450, 1497
CviAII	CATG	4 cut(s)	993, 1007, 1330, 1397
DdeI	CTNAG	3 cut(s)	241, 951, 1413
DinI	GGCGCC	1 cut(s)	150
DpnI	GATC	8 cut(s)	113, 197, 1411, 1449, 1453, 1496, 1500, 1715
DpnII	GATC	8 cut(s)	111, 195, 1409, 1447, 1451, 1494, 1498, 1713
DraIII	CACNNNGTG	1 cut(s)	69
Eam1104I	CTCTTC	1 cut(s)	714
EarI	CTCTTC	1 cut(s)	714
EciI	GGCGGA	1 cut(s)	531
Eco130I	CCWWGG	2 cut(s)	1363, 1592
Eco147I	AGGCCT	1 cut(s)	945
Eco24I	GRGCYC	2 cut(s)	1423, 1466
Eco31I	GGTCTC	1 cut(s)	873
Eco47I	GGWCC	7 cut(s)	139, 230, 570, 798, 1030, 1229, 1280
Eco47III	AGCGCT	1 cut(s)	409
Eco72I	CACGTG	1 cut(s)	66
EcoO109I	RGGNCCY	1 cut(s)	1030
EcoRII	CCWGG	1 cut(s)	1225
EcoT14I	CCWWGG	2 cut(s)	1363, 1592
EcoT38I	GRGCYC	2 cut(s)	1423, 1466
EgeI	GGCGCC	1 cut(s)	150
EheI	GGCGCC	1 cut(s)	150
ErhI	CCWWGG	2 cut(s)	1363, 1592
FaeI	CATG	4 cut(s)	996, 1010, 1333, 1400
FalI	AAGNNNNNCTT	2 cut(s)	930, 962
FaqI	GGGAC	3 cut(s)	338, 374, 1610
FatI	CATG	4 cut(s)	992, 1006, 1329, 1396
FauI	CCCGC	3 cut(s)	414, 856, 1610
FauNDI	CATATG	1 cut(s)	1276
FblI	GTMKAC	1 cut(s)	10
FokI	GGATG	7 cut(s)	201, 249, 459, 572, 1097, 1178, 1330
FriOI	GRGCYC	2 cut(s)	1423, 1466
FspBI	CTAG	2 cut(s)	6, 1593
GlaI	GCGC	3 cut(s)	150, 376, 409
GsuI	CTGGAG	1 cut(s)	503
HaeII	RGCGCY	3 cut(s)	152, 378, 411
HaeIII	GGCC	4 cut(s)	660, 745, 945, 1340
HapII	CCGG	5 cut(s)	109, 469, 476, 527, 1196
HhaI	GCGC	3 cut(s)	151, 377, 410
Hin1I	GRCGYC	1 cut(s)	149
Hin1II	CATG	4 cut(s)	996, 1010, 1333, 1400
Hin6I	GCGC	3 cut(s)	149, 375, 408
HinP1I	GCGC	3 cut(s)	149, 375, 408
HincII	GTYRAC	2 cut(s)	546, 1218
HindII	GTYRAC	2 cut(s)	546, 1218
HinfI	GANTC	4 cut(s)	563, 830, 1169, 1475
HpaII	CCGG	5 cut(s)	109, 469, 476, 527, 1196
HphI	GGTGA	6 cut(s)	198, 200, 562, 583, 1556, 1717
Hpy166II	GTNNAC	7 cut(s)	11, 72, 230, 546, 634, 895, 1218
Hpy188I	TCNGA	6 cut(s)	244, 802, 903, 1409, 1414, 1689
Hpy188III	TCNNGA	7 cut(s)	109, 476, 779, 1196, 1232, 1502, 1536
Hpy8I	GTNNAC	7 cut(s)	11, 72, 230, 546, 634, 895, 1218
Hpy99I	CGWCG	1 cut(s)	1566
HpyAV	CCTTC	8 cut(s)	243, 604, 935, 1257, 1351, 1517, 1619, 1696
HpyCH4III	ACNGT	6 cut(s)	550, 767, 814, 1111, 1324, 1723
HpyCH4IV	ACGT	1 cut(s)	65
HpyCH4V	TGCA	7 cut(s)	576, 682, 703, 1208, 1253, 1574, 1646
HpyF10VI	GCNNNNNNNGC	8 cut(s)	190, 383, 386, 460, 469, 478, 666, 1664
HpyF3I	CTNAG	3 cut(s)	241, 951, 1413
HpySE526I	ACGT	1 cut(s)	65
Hsp92I	GRCGYC	1 cut(s)	149
Hsp92II	CATG	4 cut(s)	996, 1010, 1333, 1400
HspAI	GCGC	3 cut(s)	149, 375, 408
KasI	GGCGCC	1 cut(s)	148
Kpn2I	TCCGGA	3 cut(s)	108, 475, 1195
KpnI	GGTACC	1 cut(s)	1371
KroI	GCCGGC	1 cut(s)	526
KroNI	GCCGGC	1 cut(s)	528
Kzo9I	GATC	8 cut(s)	111, 195, 1409, 1447, 1451, 1494, 1498, 1713
LguI	GCTCTTC	1 cut(s)	714
LmnI	GCTCC	3 cut(s)	478, 484, 1594
Lsp1109I	GCAGC	9 cut(s)	292, 328, 331, 364, 367, 370, 1220, 1654, 1676
LweI	GCATC	2 cut(s)	481, 1576
MaeI	CTAG	2 cut(s)	6, 1593
MaeII	ACGT	1 cut(s)	65
MaeIII	GTNAC	4 cut(s)	88, 200, 1067, 1291
MalI	GATC	8 cut(s)	113, 197, 1411, 1449, 1453, 1496, 1500, 1715
MbiI	CCGCTC	1 cut(s)	193
MboI	GATC	8 cut(s)	111, 195, 1409, 1447, 1451, 1494, 1498, 1713
MflI	RGATCY	2 cut(s)	1409, 1713
MhlI	GDGCHC	3 cut(s)	1329, 1423, 1466
MluCI	AATT	7 cut(s)	39, 100, 672, 1097, 1135, 1185, 1680
Mly113I	GGCGCC	1 cut(s)	149
MmeI	TCCRAC	2 cut(s)	188, 1667
MroI	TCCGGA	3 cut(s)	108, 475, 1195
MroNI	GCCGGC	1 cut(s)	526
MroXI	GAANNNNTTC	1 cut(s)	1509
MseI	TTAA	4 cut(s)	21, 29, 1100, 1352
MslI	CAYNNNNRTG	2 cut(s)	1011, 1109
MspA1I	CMGCKG	3 cut(s)	155, 1211, 1667
MspI	CCGG	5 cut(s)	109, 469, 476, 527, 1196
MspR9I	CCNGG	1 cut(s)	1227
Mva1269I	GAATGC	1 cut(s)	1120
MvaI	CCWGG	1 cut(s)	1227
MwoI	GCNNNNNNNGC	8 cut(s)	190, 383, 386, 460, 469, 478, 666, 1664
NaeI	GCCGGC	1 cut(s)	528
NarI	GGCGCC	1 cut(s)	149
NdeI	CATATG	1 cut(s)	1276
NdeII	GATC	8 cut(s)	111, 195, 1409, 1447, 1451, 1494, 1498, 1713
NgoMIV	GCCGGC	1 cut(s)	526
NlaIII	CATG	4 cut(s)	996, 1010, 1333, 1400
NlaIV	GGNNCC	4 cut(s)	150, 822, 998, 1369
NmuCI	GTSAC	2 cut(s)	88, 1291
PaqCI	CACCTGC	1 cut(s)	406
PceI	AGGCCT	1 cut(s)	945
PciSI	GCTCTTC	1 cut(s)	714
PcsI	WCGNNNNNNNCGW	1 cut(s)	563
PctI	GAATGC	1 cut(s)	1120
PdiI	GCCGGC	1 cut(s)	528
PdmI	GAANNNNTTC	1 cut(s)	1509
PfeI	GAWTC	4 cut(s)	563, 830, 1169, 1475
Pfl23II	CGTACG	1 cut(s)	510
PflFI	GACNNNGTC	1 cut(s)	397
PluTI	GGCGCC	1 cut(s)	152
PmaCI	CACGTG	1 cut(s)	66
PmlI	CACGTG	1 cut(s)	66
Ppu21I	YACGTR	1 cut(s)	66
PpuMI	RGGWCCY	1 cut(s)	1030
PshBI	ATTAAT	1 cut(s)	21
Psp5II	RGGWCCY	1 cut(s)	1030
Psp6I	CCWGG	1 cut(s)	1225
PspCI	CACGTG	1 cut(s)	66
PspGI	CCWGG	1 cut(s)	1225
PspLI	CGTACG	1 cut(s)	510
PspN4I	GGNNCC	4 cut(s)	150, 822, 998, 1369
PspPI	GGNCC	9 cut(s)	139, 230, 570, 659, 743, 798, 1030, 1229, 1280
PspPPI	RGGWCCY	1 cut(s)	1030
PsuI	RGATCY	2 cut(s)	1409, 1713
PsyI	GACNNNGTC	1 cut(s)	397
PvuII	CAGCTG	3 cut(s)	155, 1211, 1667
RseI	CAYNNNNRTG	2 cut(s)	1011, 1109
SapI	GCTCTTC	1 cut(s)	714
SaqAI	TTAA	4 cut(s)	21, 29, 1100, 1352
Sau3AI	GATC	8 cut(s)	111, 195, 1409, 1447, 1451, 1494, 1498, 1713
Sau96I	GGNCC	9 cut(s)	139, 230, 570, 659, 743, 798, 1030, 1229, 1280
ScaI	AGTACT	1 cut(s)	769
ScrFI	CCNGG	1 cut(s)	1227
SduI	GDGCHC	3 cut(s)	1329, 1423, 1466
SfaNI	GCATC	2 cut(s)	481, 1576
SfcI	CTRYAG	2 cut(s)	250, 763
SfoI	GGCGCC	1 cut(s)	150
SinI	GGWCC	7 cut(s)	139, 230, 570, 798, 1030, 1229, 1280
SmiMI	CAYNNNNRTG	2 cut(s)	1011, 1109
SmlI	CTYRAG	1 cut(s)	930
SmoI	CTYRAG	1 cut(s)	930
SpeI	ACTAGT	1 cut(s)	5
Sse9I	AATT	7 cut(s)	39, 100, 672, 1097, 1135, 1185, 1680
SseBI	AGGCCT	1 cut(s)	945
SsiI	CCGC	9 cut(s)	193, 309, 336, 372, 387, 421, 516, 863, 1617
SspDI	GGCGCC	1 cut(s)	148
SspMI	CTAG	2 cut(s)	6, 1593
StuI	AGGCCT	1 cut(s)	945
StyD4I	CCNGG	1 cut(s)	1225
StyI	CCWWGG	2 cut(s)	1363, 1592
TaaI	ACNGT	6 cut(s)	550, 767, 814, 1111, 1324, 1723
TaiI	ACGT	1 cut(s)	68
TasI	AATT	7 cut(s)	39, 100, 672, 1097, 1135, 1185, 1680
TatI	WGTACW	5 cut(s)	748, 767, 1125, 1154, 1175
TauI	GCSGC	3 cut(s)	311, 375, 389
TfiI	GAWTC	4 cut(s)	563, 830, 1169, 1475
Tru1I	TTAA	4 cut(s)	21, 29, 1100, 1352
Tru9I	TTAA	4 cut(s)	21, 29, 1100, 1352
TscAI	CASTG	2 cut(s)	959, 1116
TseFI	GTSAC	2 cut(s)	88, 1291
TseI	GCWGC	9 cut(s)	305, 341, 344, 377, 380, 383, 1208, 1664, 1667
Tsp45I	GTSAC	2 cut(s)	88, 1291
TspDTI	ATGAA	5 cut(s)	576, 873, 995, 1182, 1389
TspGWI	ACGGA	6 cut(s)	76, 182, 215, 572, 854, 1178
TspRI	CASTG	2 cut(s)	959, 1116
Tth111I	GACNNNGTC	1 cut(s)	397
VpaK11BI	GGWCC	7 cut(s)	139, 230, 570, 798, 1030, 1229, 1280
VspI	ATTAAT	1 cut(s)	21
XapI	RAATTY	4 cut(s)	39, 100, 1135, 1185
XmaJI	CCTAGG	1 cut(s)	1592
XmiI	GTMKAC	1 cut(s)	10
XmnI	GAANNNNTTC	1 cut(s)	1509
XspI	CTAG	2 cut(s)	6, 1593
ZrmI	AGTACT	1 cut(s)	769

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.