pycom12g17950
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002297
Physical Location & Seq
Forward (+)
20259510 .. 20264691
5182 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2862 bp
ATGCTAAAGATGAGAGTTTCTGGATGTGTCGTTGCTCTCCTGTGTTCTTGCTTCGTGCTGTTTGCCGTTGCAGAAGTTACAAATCCTTCAGAAGTCAATGTACTGAGAGCAGTCAAGAGGAAATTAATTGATCCTAAGAATCATCTAAGGAATTGGGACGACGGGGATCCTTGCGAATCTTATTGGACCGGAGTTTTCTGTTTTGATGCTGTTGGGGATGATGGATACTTGCACCTCCGGGAACTCCGACTGCTGAATATGAATCTCTCAGGAACTTTAGCACCTGAGCTTGGCCAACTATCCCAACTTCAAATATTAGATTTCATGTGGAATGAATTAAGTGGAACTATACCGAATGCGATCGGAAGCATTACATCCTTGAAACTCTTGCTCTTGAATGGAAACAAACTATCTGGTTCCTTACCTCCTGAGCTCGGTTATCTTACACACTTAACTAGACTACAAGTCGATCAGAACCATATGTCAGGTCCAATTCCAAAATCATTTGCTAACATGGCCAGTTTAAAACATCTCCACTTGAACAACAACTCCTTCAGCGGTCAAATTCCGCCTGAGCTTTCTAAAGCACTCAATCTTCTTCACTTGCTTTTCGATAATAATAACTTTTCCGGACATGTACCACCAGAATTCTCCATGTTACCGAACGCGCGCATCATTCAGTTCGATAACAACAACTTCAGGGGGACTGAGATTCCAGCCTCTTACGGAAACATATCCAGATTAACAAAACTAAGTCTTAGGAATTGCAGTTTGCAGGGAGAAATTCCTGACCTTAGCAGGATAGCTAACCTTAGTTATATTGATCTAAGCCGGAATCAACTTTCTGGATCCATACCGTCACACAGACTTTCTGTCAATATAACAACAATCATTCTGTCAGACAACCGTCTTAATGGATCGATTCCTGAGAGTTTTTATGATCTTCCAGCTCTTCAGAAAGTGTCGGTCCACGACAATTTTATTACGGGTTCTGTCCCTGCTATGTGGCGGAAAATATCTTTCAGTACAAGAGCTAGACTTGCACTTGATCTTCGGAACAATTTGCTTTCACAAATATCGGAAGAGCTAAATCCTCCTGCAAATGTCACCTTGAGGCTTGAAGGAAATCCTGTCTGCAAAAATGCAAGTGTACCAAATATAGGCCAGTTCTGTCGATCTGAAGCTGGAGGAGACGGGATCTCTGAAAACTCGATAAACTCCACTCAACCAATGACCTGCTCTAGCCAAGCATGTCCAACAGACTATTTCTACGAATATGTCCCATCTTCCCCAGTTCCATGCTTTTGTGCATCACCTTTAAGAGTTACATATCGTCTAAAAAGTCCGAGTTTTTCATATTTTGCTCCTTATAAACACAATTTTGAAATCTACTTAACTGGCGCTCTCAACCTGCACCTTTATCAATTATCAATTGATTCATTTGTCTGGCAAGACGGGCCTCGTTTGCTGATGCATATGAAGTTTTTTCCTATGTTCATCAATCCACACGTGAATTTATTTAATGCAACTGAGGTTCAGCGATTGAGAGGCCGGTTTACATCATGGGATTTTCCTCCAACTGATTTCTTTGGACCGTATGAGCTTCTCAATTTCACTCTGCTGGGACCTTATTCGAACATGATTGTTGAGCAGCAAAAGACGGGCATTAGCAAGAGAGCTTTAGCAGGTGTTATAATAGGATGCATTGCTGCTGTTGTCATTATTCCTGCAGTAGTCATGCTTCTGACCAAAAGATATGTCAAACACCGTTACCCACCACCTTCACGGAGATATTCATCCTTAAAGATCTCTATGAGAATTGAAGGTGTAAAGGCGTTCACCTTCAAAGAAATGAAGGTCGCTACTGGGAATTTCGATACTTCAATGCAACTTGGACGAGGAGGCTATGGGAAAGTTTACAAAGGCATTTTGTCCGATAACACAGTTGTAGCCGTAAAGCGTGCAGAAGAAGGATCCTTACAGGGCGGAAGGGAGTTCTTGACCGAGATAGAACTGCTATCAAGGGTACATCACCGAAACCTGGTCTCTTTGGTGGGATATTGTGATGAAGAAGGGGAGCAGATGCTGGTTTACGAGTTTATGCCTAATGGTACCTTACGCGACTGGCTTTGGGTTAAAGCCAAGGGAAGCCTGGACTTCACTATGAGGTTACGTATTGCATTAGGTTCCGCTAAAGGCATCCTTTATCTCCATACGGAGGCAAATCCTCCAATATTCCACCGGGATATCAAAGCCAGCAACATACTCTTGGACTCCAACCTCGTGGCCAAAGTGGCGGATTTTGGACTCTCGCGACTTGCACCTCTGCAGGATGATGAAGGGACCGGGTCCTCTTATGTATCTACAGTCGTGAGAGGAACGGTTACGTTGATCGATCATGAATATTTCCTGACCAATAAGTTGACGGACAAAAGCGATGTCTATAGCCTAGGAATCGTGTTTCTGGAGCTCCTTACCGGCGATCAGCCAATTTCACACGGCAAAAACATAGTTCGAGAGGTGAATCTGGCTTATCAGGCAGGGCTGGTGTTCTCAATCATAGACAAAAGAATGGGATCCTATCCATCCAAATGTGTTGAGAGGTTTCTTGATTTGGCCCTCAGATGTTGCAACGAAAAGCCAGATAAACGGCCTCATATGCTGGAAGTGGTGAGGGAGATCGAAAACATACTCAAAATCATGCCGGCAACCGACACCATATTCTCGCCATCTACTACCTCGCACAGTGACCAGTCACCAACCTCGTCGTCCTTCATGACCGGAGACCGATCCTACGTGTCCTCCAGCCTGGCAGGAAGTGATCTAACCAGTGGCGTTGTCCCGACCATAACACCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

954

Amino Acids

106.09

Weight (kDa)

8.3

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 67 6.2e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 78 - 204 8.2e-10 Leucine-rich repeat region
Pkinase PF00069 627 - 892 6.1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 628 - 893 5.6e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1371, 1694
AarI CACCTGC 1 cut(s) 1676
Acc36I ACCTGC 3 cut(s) 1244, 1419, 1676
Acc65I GGTACC 1 cut(s) 2111
AccB1I GGYRCC 1 cut(s) 2111
AccII CGCG 4 cut(s) 668, 670, 2121, 2314
AccIII TCCGGA 1 cut(s) 629
AciI CCGC 6 cut(s) 558, 569, 1009, 1986, 2190, 2297
AcoI YGGCCR 3 cut(s) 292, 516, 2286
AcsI RAATTY 5 cut(s) 564, 647, 783, 1513, 1870
AcuI CTGAAG 5 cut(s) 72, 538, 682, 938, 1200
AcvI CACGTG 1 cut(s) 1510
AfaI GTAC 6 cut(s) 102, 639, 1027, 1152, 2028, 2113
AfiI CCNNNNNNNGG 5 cut(s) 290, 434, 1160, 2041, 2218
AflIII ACRYGT 3 cut(s) 634, 1507, 2796
AgsI TTSAA 9 cut(s) 311, 382, 397, 541, 1121, 1385, 1823, 1846, 1884
AhdI GACNNNNNGTC 2 cut(s) 464, 872
AjnI CCWGG 3 cut(s) 2040, 2151, 2808
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
Alw21I GWGCWC 2 cut(s) 435, 2472
Alw26I GTCTC 3 cut(s) 1185, 2050, 2778
AlwNI CAGNNNCTG 1 cut(s) 2086
Aor13HI TCCGGA 1 cut(s) 629
AoxI GGCC 8 cut(s) 292, 516, 1162, 1457, 1549, 2286, 2616, 2651
ApeKI GCWGC 2 cut(s) 1651, 1709
ApoI RAATTY 5 cut(s) 564, 647, 783, 1513, 1870
ArsI GACNNNNNNTTYG 4 cut(s) 1743, 1775, 2424, 2456
AseI ATTAAT 1 cut(s) 125
Asp718I GGTACC 1 cut(s) 2111
AspA2I CCTAGG 1 cut(s) 2449
AspLEI GCGC 3 cut(s) 670, 672, 1403
AspS9I GGNCC 9 cut(s) 186, 488, 967, 1457, 1592, 1625, 2343, 2349, 2617
AsuC2I CCSGG 3 cut(s) 239, 2243, 2347
AsuHPI GGTGA 7 cut(s) 1099, 1305, 1831, 2024, 2533, 2683, 2748
AsuII TTCGAA 1 cut(s) 1634
AvaII GGWCC 7 cut(s) 186, 488, 967, 1592, 1625, 2343, 2349
AvrII CCTAGG 1 cut(s) 2449
BalI TGGCCA 3 cut(s) 294, 518, 2288
BamHI GGATCC 4 cut(s) 166, 848, 1973, 2576
BanI GGYRCC 1 cut(s) 2111
BanII GRGCYC 2 cut(s) 435, 2472
BarI GAAGNNNNNNTAC 6 cut(s) 84, 116, 1725, 1757, 1962, 1994
BauI CACGAG 1 cut(s) 2282
BbrPI CACGTG 1 cut(s) 1510
Bbv12I GWGCWC 2 cut(s) 435, 2472
BbvI GCAGC 2 cut(s) 1663, 1696
BccI CCATC 4 cut(s) 215, 1291, 2593, 2737
BceAI ACGGC 4 cut(s) 50, 1937, 2515, 2666
BciT130I CCWGG 3 cut(s) 2042, 2153, 2810
BciVI GTATCC 1 cut(s) 218
BcnI CCSGG 3 cut(s) 239, 2243, 2347
BcoDI GTCTC 3 cut(s) 1185, 2050, 2778
BfaI CTAG 4 cut(s) 456, 1035, 1242, 2450
BfmI CTRYAG 4 cut(s) 1728, 2327, 2364, 2443
BfoI RGCGCY 1 cut(s) 1404
BfuAI ACCTGC 3 cut(s) 1244, 1419, 1676
BfuI GTATCC 1 cut(s) 218
BglI GCCNNNNNGGC 1 cut(s) 2294
BglII AGATCT 1 cut(s) 1806
BisI GCNGC 2 cut(s) 1652, 1710
BlnI CCTAGG 1 cut(s) 2449
BlsI GCNGC 2 cut(s) 1653, 1711
Bme1390I CCNGG 6 cut(s) 239, 2042, 2153, 2243, 2347, 2810
Bme18I GGWCC 7 cut(s) 186, 488, 967, 1592, 1625, 2343, 2349
BmeRI GACNNNNNGTC 2 cut(s) 464, 872
BmgT120I GGNCC 9 cut(s) 186, 488, 967, 1457, 1592, 1625, 2343, 2349, 2617
BmrFI CCNGG 6 cut(s) 239, 2042, 2153, 2243, 2347, 2810
BmrI ACTGGG 2 cut(s) 1286, 1875
BmsI GCATC 7 cut(s) 196, 681, 1319, 1461, 1691, 2073, 2208
BmuI ACTGGG 2 cut(s) 1286, 1875
BoxI GACNNNNGTC 1 cut(s) 906
BpmI CTGGAG 3 cut(s) 1206, 2486, 2788
Bpu10I CCTNAGC 4 cut(s) 285, 429, 573, 794
Bpu14I TTCGAA 1 cut(s) 1634
BpuEI CTTGAG 1 cut(s) 1132
BpuMI CCSGG 3 cut(s) 239, 2243, 2347
Bsa29I ATCGAT 2 cut(s) 920, 2394
BsaAI YACGTR 3 cut(s) 1510, 2174, 2797
BsaBI GATNNNNATC 1 cut(s) 1795
BsaI GGTCTC 2 cut(s) 2050, 2778
BsaJI CCNNGG 2 cut(s) 2142, 2449
BsaWI WCCGGW 3 cut(s) 188, 629, 2780
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 2786, 2816
Bsc4I CCNNNNNNNGG 5 cut(s) 290, 434, 1160, 2041, 2218
Bse118I RCCGGY 3 cut(s) 1551, 2477, 2704
Bse1I ACTGG 8 cut(s) 519, 1165, 1292, 1402, 1870, 2129, 2752, 2829
Bse3DI GCAATG 1 cut(s) 1704
Bse8I GATNNNNATC 1 cut(s) 1795
BseAI TCCGGA 1 cut(s) 629
BseBI CCWGG 3 cut(s) 2042, 2153, 2810
BseCI ATCGAT 2 cut(s) 920, 2394
BseDI CCNNGG 2 cut(s) 2142, 2449
BseGI GGATG 8 cut(s) 29, 223, 374, 1706, 1796, 2199, 2338, 2585
BseJI GATNNNNATC 1 cut(s) 1795
BseLI CCNNNNNNNGG 5 cut(s) 290, 434, 1160, 2041, 2218
BseMI GCAATG 1 cut(s) 1704
BseMII CTCAG 9 cut(s) 95, 276, 282, 420, 564, 699, 918, 1521, 2635
BseNI ACTGG 8 cut(s) 519, 1165, 1292, 1402, 1870, 2129, 2752, 2829
BsePI GCGCGC 1 cut(s) 668
BseRI GAGGAG 2 cut(s) 1203, 1914
BseXI GCAGC 2 cut(s) 1663, 1696
BseYI CCCAGC 1 cut(s) 1621
BsgI GTGCAG 2 cut(s) 1397, 1983
Bsh1236I CGCG 4 cut(s) 668, 670, 2121, 2314
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 8 cut(s) 294, 518, 1164, 1459, 1551, 2288, 2618, 2653
BshNI GGYRCC 1 cut(s) 2111
BshVI ATCGAT 2 cut(s) 920, 2394
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 2 cut(s) 435, 2472
BslFI GGGAC 7 cut(s) 170, 718, 980, 1265, 1638, 2356, 2825
BslI CCNNNNNNNGG 5 cut(s) 290, 434, 1160, 2041, 2218
BsmAI GTCTC 3 cut(s) 1185, 2050, 2778
BsmBI CGTCTC 1 cut(s) 1185
BsmFI GGGAC 7 cut(s) 170, 718, 980, 1265, 1638, 2356, 2825
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 8 cut(s) 294, 518, 1164, 1459, 1551, 2288, 2618, 2653
Bso31I GGTCTC 2 cut(s) 2050, 2778
Bsp119I TTCGAA 1 cut(s) 1634
Bsp1286I GDGCHC 2 cut(s) 435, 2472
Bsp13I TCCGGA 1 cut(s) 629
Bsp68I TCGCGA 1 cut(s) 2314
BspACI CCGC 6 cut(s) 558, 569, 1009, 1986, 2190, 2297
BspANI GGCC 8 cut(s) 294, 518, 1164, 1459, 1551, 2288, 2618, 2653
BspCNI CTCAG 9 cut(s) 96, 277, 281, 421, 565, 700, 919, 1522, 2634
BspDI ATCGAT 2 cut(s) 920, 2394
BspEI TCCGGA 1 cut(s) 629
BspFNI CGCG 4 cut(s) 668, 670, 2121, 2314
BspHI TCATGA 2 cut(s) 2398, 2775
BspMAI CTGCAG 2 cut(s) 1732, 2331
BspMI ACCTGC 3 cut(s) 1244, 1419, 1676
BspQI GCTCTTC 2 cut(s) 957, 1077
BspT104I TTCGAA 1 cut(s) 1634
BspT107I GGYRCC 1 cut(s) 2111
BspTNI GGTCTC 2 cut(s) 2050, 2778
BsrDI GCAATG 1 cut(s) 1704
BsrFI RCCGGY 3 cut(s) 1551, 2477, 2704
BsrI ACTGG 8 cut(s) 519, 1165, 1292, 1402, 1870, 2129, 2752, 2829
BssAI RCCGGY 3 cut(s) 1551, 2477, 2704
BssECI CCNNGG 2 cut(s) 2142, 2449
BssHII GCGCGC 1 cut(s) 668
BssSI CACGAG 1 cut(s) 2282
BssT1I CCWWGG 2 cut(s) 2142, 2449
Bst2BI CACGAG 1 cut(s) 2282
Bst2UI CCWGG 3 cut(s) 2042, 2153, 2810
Bst4CI ACNGT 8 cut(s) 858, 908, 1596, 1769, 1945, 2368, 2383, 2747
Bst6I CTCTTC 2 cut(s) 957, 1077
BstBAI YACGTR 3 cut(s) 1510, 2174, 2797
BstBI TTCGAA 1 cut(s) 1634
BstC8I GCNNGC 4 cut(s) 670, 1962, 2257, 2706
BstF5I GGATG 8 cut(s) 29, 223, 374, 1706, 1796, 2199, 2338, 2585
BstFNI CGCG 4 cut(s) 668, 670, 2121, 2314
BstH2I RGCGCY 1 cut(s) 1404
BstHHI GCGC 3 cut(s) 670, 672, 1403
BstMAI GTCTC 3 cut(s) 1185, 2050, 2778
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 7 cut(s) 515, 1040, 1456, 1465, 2294, 2537, 2659
BstNI CCWGG 3 cut(s) 2042, 2153, 2810
BstNSI RCATGY 2 cut(s) 638, 1254
BstPAI GACNNNNGTC 1 cut(s) 906
BstSCI CCNGG 6 cut(s) 237, 2040, 2151, 2241, 2345, 2808
BstSFI CTRYAG 4 cut(s) 1728, 2327, 2364, 2443
BstSNI TACGTA 1 cut(s) 2174
BstUI CGCG 4 cut(s) 668, 670, 2121, 2314
BstV1I GCAGC 2 cut(s) 1663, 1696
BstX2I RGATCY 6 cut(s) 166, 848, 1197, 1806, 1973, 2576
BstXI CCANNNNNNTGG 1 cut(s) 2284
BstYI RGATCY 6 cut(s) 166, 848, 1197, 1806, 1973, 2576
Bsu15I ATCGAT 2 cut(s) 920, 2394
BsuI GTATCC 1 cut(s) 218
BsuRI GGCC 8 cut(s) 294, 518, 1164, 1459, 1551, 2288, 2618, 2653
BsuTUI ATCGAT 2 cut(s) 920, 2394
BtgZI GCGATG 1 cut(s) 2451
BtsCI GGATG 8 cut(s) 29, 223, 374, 1706, 1796, 2199, 2338, 2585
BtsIMutI CAGTG 2 cut(s) 2752, 2836
BtuMI TCGCGA 1 cut(s) 2314
BveI ACCTGC 3 cut(s) 1244, 1419, 1676
Cac8I GCNNGC 4 cut(s) 670, 1962, 2257, 2706
CaiI CAGNNNCTG 1 cut(s) 2086
CciI TCATGA 2 cut(s) 2398, 2775
CfoI GCGC 3 cut(s) 670, 672, 1403
Cfr10I RCCGGY 3 cut(s) 1551, 2477, 2704
Cfr13I GGNCC 9 cut(s) 186, 488, 967, 1457, 1592, 1625, 2343, 2349, 2617
ClaI ATCGAT 2 cut(s) 920, 2394
CsiI ACCWGGT 1 cut(s) 2040
Csp6I GTAC 6 cut(s) 101, 638, 1026, 1151, 2027, 2112
CviQI GTAC 6 cut(s) 101, 638, 1026, 1151, 2027, 2112
DraI TTTAAA 1 cut(s) 525
DriI GACNNNNNGTC 2 cut(s) 464, 872
EaeI YGGCCR 3 cut(s) 292, 516, 2286
Eam1104I CTCTTC 2 cut(s) 957, 1077
Eam1105I GACNNNNNGTC 2 cut(s) 464, 872
EarI CTCTTC 2 cut(s) 957, 1077
EciI GGCGGA 4 cut(s) 558, 1024, 2001, 2312
Ecl136II GAGCTC 2 cut(s) 433, 2470
Eco105I TACGTA 1 cut(s) 2174
Eco130I CCWWGG 2 cut(s) 2142, 2449
Eco24I GRGCYC 2 cut(s) 435, 2472
Eco31I GGTCTC 2 cut(s) 2050, 2778
Eco32I GATATC 1 cut(s) 2248
Eco47I GGWCC 7 cut(s) 186, 488, 967, 1592, 1625, 2343, 2349
Eco53kI GAGCTC 2 cut(s) 433, 2470
Eco57I CTGAAG 5 cut(s) 72, 538, 682, 938, 1200
Eco72I CACGTG 1 cut(s) 1510
EcoICRI GAGCTC 2 cut(s) 433, 2470
EcoO109I RGGNCCY 2 cut(s) 1625, 2349
EcoRI GAATTC 1 cut(s) 647
EcoRII CCWGG 3 cut(s) 2040, 2151, 2808
EcoRV GATATC 1 cut(s) 2248
EcoT14I CCWWGG 2 cut(s) 2142, 2449
EcoT22I ATGCAT 2 cut(s) 1476, 1706
EcoT38I GRGCYC 2 cut(s) 435, 2472
ErhI CCWWGG 2 cut(s) 2142, 2449
Esp3I CGTCTC 1 cut(s) 1185
FalI AAGNNNNNCTT 2 cut(s) 2187, 2219
FaqI GGGAC 7 cut(s) 170, 718, 980, 1265, 1638, 2356, 2825
FauNDI CATATG 3 cut(s) 480, 1476, 2658
Fnu4HI GCNGC 2 cut(s) 1652, 1710
FokI GGATG 8 cut(s) 36, 230, 361, 1713, 1783, 2186, 2345, 2572
FriOI GRGCYC 2 cut(s) 435, 2472
Fsp4HI GCNGC 2 cut(s) 1652, 1710
FspBI CTAG 4 cut(s) 456, 1035, 1242, 2450
GlaI GCGC 3 cut(s) 669, 671, 1402
GluI GCNGC 2 cut(s) 1652, 1710
GsaI CCCAGC 1 cut(s) 1625
GsuI CTGGAG 3 cut(s) 1206, 2486, 2788
HaeII RGCGCY 1 cut(s) 1404
HaeIII GGCC 8 cut(s) 294, 518, 1164, 1459, 1551, 2288, 2618, 2653
HhaI GCGC 3 cut(s) 670, 672, 1403
Hin6I GCGC 3 cut(s) 668, 670, 1401
HinP1I GCGC 3 cut(s) 668, 670, 1401
HincII GTYRAC 1 cut(s) 2424
HindII GTYRAC 1 cut(s) 2424
HphI GGTGA 7 cut(s) 1099, 1305, 1831, 2024, 2533, 2683, 2748
Hpy166II GTNNAC 7 cut(s) 970, 1151, 1557, 1839, 1918, 2092, 2424
Hpy8I GTNNAC 7 cut(s) 970, 1151, 1557, 1839, 1918, 2092, 2424
Hpy99I CGWCG 2 cut(s) 164, 2770
HpyCH4III ACNGT 8 cut(s) 858, 908, 1596, 1769, 1945, 2368, 2383, 2747
HpyCH4IV ACGT 4 cut(s) 1509, 2173, 2387, 2796
HpyF10VI GCNNNNNNNGC 7 cut(s) 515, 1040, 1456, 1465, 2294, 2537, 2659
HpySE526I ACGT 4 cut(s) 1509, 2173, 2387, 2796
HspAI GCGC 3 cut(s) 668, 670, 1401
Kpn2I TCCGGA 1 cut(s) 629
KpnI GGTACC 1 cut(s) 2115
KroI GCCGGC 1 cut(s) 2704
KroNI GCCGGC 1 cut(s) 2706
LguI GCTCTTC 2 cut(s) 957, 1077
LmnI GCTCC 4 cut(s) 1369, 2077, 2467, 2475
Lsp1109I GCAGC 2 cut(s) 1663, 1696
LweI GCATC 7 cut(s) 196, 681, 1319, 1461, 1691, 2073, 2208
MabI ACCWGGT 1 cut(s) 2040
MaeI CTAG 4 cut(s) 456, 1035, 1242, 2450
MaeII ACGT 4 cut(s) 1509, 2173, 2387, 2796
MboII GAAGA 9 cut(s) 587, 590, 935, 944, 1043, 1094, 1278, 1979, 2081
MfeI CAATTG 1 cut(s) 1431
MflI RGATCY 6 cut(s) 166, 848, 1197, 1806, 1973, 2576
MhlI GDGCHC 2 cut(s) 435, 2472
MlsI TGGCCA 3 cut(s) 294, 518, 2288
MluNI TGGCCA 3 cut(s) 294, 518, 2288
MlyI GAGTC 2 cut(s) 2267, 2301
MmeI TCCRAC 4 cut(s) 271, 1280, 1601, 2301
Mox20I TGGCCA 3 cut(s) 294, 518, 2288
Mph1103I ATGCAT 2 cut(s) 1476, 1706
MroI TCCGGA 1 cut(s) 629
MroNI GCCGGC 1 cut(s) 2704
MscI TGGCCA 3 cut(s) 294, 518, 2288
MslI CAYNNNNRTG 2 cut(s) 2590, 2857
Msp20I TGGCCA 3 cut(s) 294, 518, 2288
MspA1I CMGCKG 1 cut(s) 558
MspR9I CCNGG 6 cut(s) 239, 2042, 2153, 2243, 2347, 2810
MunI CAATTG 1 cut(s) 1431
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 3 cut(s) 2042, 2153, 2810
MvnI CGCG 4 cut(s) 668, 670, 2121, 2314
MwoI GCNNNNNNNGC 7 cut(s) 515, 1040, 1456, 1465, 2294, 2537, 2659
NaeI GCCGGC 1 cut(s) 2706
NciI CCSGG 3 cut(s) 239, 2243, 2347
NdeI CATATG 3 cut(s) 480, 1476, 2658
NgoMIV GCCGGC 1 cut(s) 2704
NmuCI GTSAC 4 cut(s) 858, 1105, 2747, 2754
NruI TCGCGA 1 cut(s) 2314
NsiI ATGCAT 2 cut(s) 1476, 1706
NspI RCATGY 2 cut(s) 638, 1254
NspV TTCGAA 1 cut(s) 1634
PagI TCATGA 2 cut(s) 2398, 2775
PaqCI CACCTGC 1 cut(s) 1676
PauI GCGCGC 1 cut(s) 668
PciI ACATGT 1 cut(s) 634
PciSI GCTCTTC 2 cut(s) 957, 1077
PctI GAATGC 1 cut(s) 361
PdiI GCCGGC 1 cut(s) 2706
PfeI GAWTC 9 cut(s) 139, 176, 262, 712, 835, 922, 1436, 2454, 2524
PflFI GACNNNGTC 1 cut(s) 2347
PfoI TCCNGGA 1 cut(s) 237
PkrI GCNGC 2 cut(s) 1653, 1711
Ple19I CGATCG 1 cut(s) 363
PleI GAGTC 2 cut(s) 2267, 2301
PmaCI CACGTG 1 cut(s) 1510
PmlI CACGTG 1 cut(s) 1510
PpsI GAGTC 2 cut(s) 2267, 2301
Ppu21I YACGTR 3 cut(s) 1510, 2174, 2797
PpuMI RGGWCCY 2 cut(s) 1625, 2349
PscI ACATGT 1 cut(s) 634
PshAI GACNNNNGTC 1 cut(s) 906
PshBI ATTAAT 1 cut(s) 125
PsiI TTATAA 2 cut(s) 1371, 1694
Psp124BI GAGCTC 2 cut(s) 435, 2472
Psp5II RGGWCCY 2 cut(s) 1625, 2349
Psp6I CCWGG 3 cut(s) 2040, 2151, 2808
PspCI CACGTG 1 cut(s) 1510
PspFI CCCAGC 1 cut(s) 1621
PspGI CCWGG 3 cut(s) 2040, 2151, 2808
PspPI GGNCC 9 cut(s) 186, 488, 967, 1457, 1592, 1625, 2343, 2349, 2617
PspPPI RGGWCCY 2 cut(s) 1625, 2349
PstI CTGCAG 2 cut(s) 1732, 2331
PstNI CAGNNNCTG 1 cut(s) 2086
PsuI RGATCY 6 cut(s) 166, 848, 1197, 1806, 1973, 2576
PsyI GACNNNGTC 1 cut(s) 2347
PteI GCGCGC 1 cut(s) 668
PvuI CGATCG 1 cut(s) 363
RruI TCGCGA 1 cut(s) 2314
RsaI GTAC 6 cut(s) 102, 639, 1027, 1152, 2028, 2113
RsaNI GTAC 6 cut(s) 101, 638, 1026, 1151, 2027, 2112
RseI CAYNNNNRTG 2 cut(s) 2590, 2857
SacI GAGCTC 2 cut(s) 435, 2472
SapI GCTCTTC 2 cut(s) 957, 1077
SatI GCNGC 2 cut(s) 1652, 1710
Sau96I GGNCC 9 cut(s) 186, 488, 967, 1457, 1592, 1625, 2343, 2349, 2617
SchI GAGTC 2 cut(s) 2267, 2301
ScrFI CCNGG 6 cut(s) 239, 2042, 2153, 2243, 2347, 2810
SduI GDGCHC 2 cut(s) 435, 2472
SexAI ACCWGGT 1 cut(s) 2040
SfaNI GCATC 7 cut(s) 196, 681, 1319, 1461, 1691, 2073, 2208
SfcI CTRYAG 4 cut(s) 1728, 2327, 2364, 2443
SfuI TTCGAA 1 cut(s) 1634
SinI GGWCC 7 cut(s) 186, 488, 967, 1592, 1625, 2343, 2349
SmiMI CAYNNNNRTG 2 cut(s) 2590, 2857
SmlI CTYRAG 1 cut(s) 1111
SmoI CTYRAG 1 cut(s) 1111
SnaBI TACGTA 1 cut(s) 2174
SsiI CCGC 6 cut(s) 558, 569, 1009, 1986, 2190, 2297
SspI AATATT 3 cut(s) 315, 2235, 2405
SspMI CTAG 4 cut(s) 456, 1035, 1242, 2450
SstI GAGCTC 2 cut(s) 435, 2472
StyD4I CCNGG 6 cut(s) 237, 2040, 2151, 2241, 2345, 2808
StyI CCWWGG 2 cut(s) 2142, 2449
TaaI ACNGT 8 cut(s) 858, 908, 1596, 1769, 1945, 2368, 2383, 2747
TaiI ACGT 4 cut(s) 1512, 2176, 2390, 2799
TaqII GACCGA 3 cut(s) 955, 2018, 2802
TatI WGTACW 2 cut(s) 100, 1025
TfiI GAWTC 9 cut(s) 139, 176, 262, 712, 835, 922, 1436, 2454, 2524
TscAI CASTG 2 cut(s) 2752, 2836
TseFI GTSAC 4 cut(s) 858, 1105, 2747, 2754
TseI GCWGC 2 cut(s) 1651, 1709
Tsp45I GTSAC 4 cut(s) 858, 1105, 2747, 2754
TspGWI ACGGA 4 cut(s) 741, 1801, 2231, 2441
TspRI CASTG 2 cut(s) 2752, 2836
Tth111I GACNNNGTC 1 cut(s) 2347
VpaK11BI GGWCC 7 cut(s) 186, 488, 967, 1592, 1625, 2343, 2349
VspI ATTAAT 1 cut(s) 125
XapI RAATTY 5 cut(s) 564, 647, 783, 1513, 1870
XceI RCATGY 2 cut(s) 638, 1254
XcmI CCANNNNNNNNNTGG 1 cut(s) 2149
XmaJI CCTAGG 1 cut(s) 2449
XspI CTAG 4 cut(s) 456, 1035, 1242, 2450
Zsp2I ATGCAT 2 cut(s) 1476, 1706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.