pycom13g00520

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003358
Physical Location & Seq
Forward (+)
318650 .. 320155
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1197 bp
ATGGCGTTCACAATAACAATGCTATCATGGAGCACAATAGAGATGGTCAGCCAGCTCCAGGCAAAGCAAGAGCTCGCAAACGCCTTGCTCGCCATCAAGTGGGGAACCGATTACTTGATCAAAGCACATCCACAGCCGGACGTGCTCTACGCGCAAGTTGGACAGTCCGATTCTGACCATGGATGTTGGCAAAGACCGGAAGACATGACCACCCCGAGAACCTCCTTCAAAATTGATGCTCAACACCCTGGCTCCGATCTTGCTGCTGAAACTGCTGCTGCTTTTGCCGCTGCTTCAATTGCTTTCAAGAATTCGGACTCTCAATACTCGTCCACGCTTCTAATGCACGCGAAACAACTTTTTGATTTTGCCCGCGATCACCCCGGTCTTTATCAGGACAGCATCCCTTATGCCGGAAAAAGCTACAGCAGTTCCGGTTATCATGTAAGAGATGAAATGTTGTGGGCAGCAGCATGGCTGCATCGCGCTACAAATGAAAAACCGTACCTGGATTACGTTGAGCAAGCACAAGATATAGGGGGCACAAGATATGCATTCTCATGGGATGACAAATACCTCGGCGCACAGCTCTTGGTTGCAATGCTTGTACTGGAGGGGAAGGTTGACAATGCAGGAAAATGGGCAGAATACAGGAGCCACGCAGAGGAGTTCATTTGTTCGTGCATCCAGAAAGGAAGCATGAACAGGAAGCGTACACCCGGAGGTTTGCTATCGTTTGCGCAGTGGGACAACATTCAATGCGTCGCCACTGCTACCTTCGCCACCATTGTTTATTCCAATTACTTGGCCGCCAAAAATGCCTCCGTCAACTGTGCTGCTGGCAACGCTAACCCTTTTGATCTTGCTGCATTCGTCAAATCACAGGTGGATTATATATTGGGGTCAAACCCCAAGAACATGAGTTACATGGTTGGATATGGAGCAAATTATCCGAAGCGGATTCATCACAGAGGTGCATCAATTATATCAATCAAGCAGGATCCGACACCAGTGACATGCGGCGGAGGGTACACTTGGTGCCACAGCAACGCACCGAACCCAAATGTGCTAGATGGAGCTGTCGTGGGCCCAGATAAAAATGATGGTTTCTCCGACGATAGAGGCAATTTTCAACTTGGTGAACCCACAACTTATAACATCGGTCCATTAGTTGGTGTCTTCGCTTACTTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

399

Amino Acids

43.66

Weight (kDa)

5.86

Isoelectric Point (pI)

29.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 393 2.3e-121 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1155
Acc16I TGCGCA 1 cut(s) 741
AccB1I GGYRCC 1 cut(s) 1038
AccB7I CCANNNNNTGG 2 cut(s) 99, 1172
AccII CGCG 4 cut(s) 152, 350, 375, 486
AciI CCGC 6 cut(s) 288, 373, 810, 958, 1020, 1023
AclWI GGATC 2 cut(s) 995, 1008
AcoI YGGCCR 1 cut(s) 807
AcsI RAATTY 1 cut(s) 310
AdeI CACNNNGTG 1 cut(s) 1038
AfaI GTAC 4 cut(s) 506, 609, 715, 1031
AfiI CCNNNNNNNGG 5 cut(s) 58, 99, 413, 664, 1172
AgsI TTSAA 5 cut(s) 229, 297, 307, 758, 1133
AjiI CACGTC 1 cut(s) 142
AjnI CCWGG 3 cut(s) 57, 247, 507
AleI CACNNNNGTG 1 cut(s) 972
AluBI AGCT 5 cut(s) 55, 73, 423, 589, 1079
AluI AGCT 5 cut(s) 55, 73, 423, 589, 1079
Alw21I GWGCWC 3 cut(s) 35, 75, 147
AlwI GGATC 2 cut(s) 995, 1008
Ama87I CYCGRG 1 cut(s) 214
AoxI GGCC 2 cut(s) 807, 1087
ApaI GGGCCC 1 cut(s) 1091
ApeKI GCWGC 9 cut(s) 263, 275, 278, 290, 467, 470, 478, 836, 866
ApoI RAATTY 1 cut(s) 310
AspLEI GCGC 4 cut(s) 154, 488, 584, 742
AspS9I GGNCC 3 cut(s) 1087, 1088, 1163
AsuC2I CCSGG 2 cut(s) 384, 720
AsuHPI GGTGA 2 cut(s) 371, 1151
AvaI CYCGRG 1 cut(s) 214
AvaII GGWCC 1 cut(s) 1163
BaeGI GKGCMC 2 cut(s) 545, 1091
BamHI GGATCC 1 cut(s) 1000
BanI GGYRCC 1 cut(s) 1038
BanII GRGCYC 2 cut(s) 75, 1091
BbsI GAAGAC 2 cut(s) 207, 1171
Bbv12I GWGCWC 3 cut(s) 35, 75, 147
BbvI GCAGC 9 cut(s) 250, 262, 265, 277, 465, 479, 482, 823, 853
BccI CCATC 4 cut(s) 37, 101, 1067, 1097
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 3 cut(s) 59, 249, 509
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 2 cut(s) 384, 720
BfaI CTAG 1 cut(s) 1070
BfmI CTRYAG 1 cut(s) 424
Bme1390I CCNGG 5 cut(s) 59, 249, 384, 509, 720
Bme18I GGWCC 1 cut(s) 1163
BmeT110I CYCGRG 1 cut(s) 214
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 3 cut(s) 1087, 1088, 1163
BmiI GGNNCC 6 cut(s) 106, 253, 656, 1002, 1040, 1089
BmrFI CCNGG 5 cut(s) 59, 249, 384, 509, 720
BmsI GCATC 5 cut(s) 226, 411, 490, 693, 986
BpiI GAAGAC 2 cut(s) 207, 1171
BpmI CTGGAG 2 cut(s) 41, 632
BpuMI CCSGG 2 cut(s) 384, 720
BsaJI CCNNGG 4 cut(s) 178, 247, 382, 577
BsaWI WCCGGW 2 cut(s) 196, 434
BsaXI ACNNNNNCTCC 4 cut(s) 236, 266, 1068, 1098
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 99, 413, 664, 1172
Bse1I ACTGG 2 cut(s) 615, 1010
Bse3DI GCAATG 1 cut(s) 606
BseBI CCWGG 3 cut(s) 59, 249, 509
BseDI CCNNGG 4 cut(s) 178, 247, 382, 577
BseGI GGATG 5 cut(s) 127, 188, 402, 571, 684
BseLI CCNNNNNNNGG 5 cut(s) 58, 99, 413, 664, 1172
BseMI GCAATG 1 cut(s) 606
BseNI ACTGG 2 cut(s) 615, 1010
BseRI GAGGAG 1 cut(s) 680
BseSI GKGCMC 2 cut(s) 545, 1091
BseXI GCAGC 9 cut(s) 250, 262, 265, 277, 465, 479, 482, 823, 853
Bsh1236I CGCG 4 cut(s) 152, 350, 375, 486
BshFI GGCC 2 cut(s) 809, 1089
BshNI GGYRCC 1 cut(s) 1038
BsiHKAI GWGCWC 3 cut(s) 35, 75, 147
BsiHKCI CYCGRG 1 cut(s) 214
BsiSI CCGG 6 cut(s) 137, 197, 384, 414, 435, 720
BslFI GGGAC 1 cut(s) 761
BslI CCNNNNNNNGG 5 cut(s) 58, 99, 413, 664, 1172
BsmFI GGGAC 1 cut(s) 761
BsmI GAATGC 2 cut(s) 554, 869
BsnI GGCC 2 cut(s) 809, 1089
BsoBI CYCGRG 1 cut(s) 214
Bsp120I GGGCCC 1 cut(s) 1087
Bsp1286I GDGCHC 5 cut(s) 35, 75, 147, 545, 1091
Bsp143I GATC 5 cut(s) 117, 256, 376, 859, 1000
Bsp19I CCATGG 1 cut(s) 178
BspACI CCGC 6 cut(s) 288, 373, 810, 958, 1020, 1023
BspANI GGCC 2 cut(s) 809, 1089
BspFNI CGCG 4 cut(s) 152, 350, 375, 486
BspLI GGNNCC 6 cut(s) 106, 253, 656, 1002, 1040, 1089
BspPI GGATC 2 cut(s) 995, 1008
BspT107I GGYRCC 1 cut(s) 1038
BsrDI GCAATG 1 cut(s) 606
BsrI ACTGG 2 cut(s) 615, 1010
BssECI CCNNGG 4 cut(s) 178, 247, 382, 577
BssMI GATC 5 cut(s) 117, 256, 376, 859, 1000
BssT1I CCWWGG 1 cut(s) 178
Bst2UI CCWGG 3 cut(s) 59, 249, 509
Bst4CI ACNGT 3 cut(s) 165, 504, 833
BstC8I GCNNGC 7 cut(s) 53, 75, 90, 348, 373, 525, 841
BstDEI CTNAG 1 cut(s) 1194
BstDSI CCRYGG 1 cut(s) 178
BstF5I GGATG 5 cut(s) 127, 188, 402, 571, 684
BstFNI CGCG 4 cut(s) 152, 350, 375, 486
BstHHI GCGC 4 cut(s) 154, 488, 584, 742
BstKTI GATC 5 cut(s) 120, 259, 379, 862, 1003
BstMBI GATC 5 cut(s) 117, 256, 376, 859, 1000
BstNI CCWGG 3 cut(s) 59, 249, 509
BstNSI RCATGY 1 cut(s) 1020
BstSCI CCNGG 5 cut(s) 57, 247, 382, 507, 718
BstSFI CTRYAG 1 cut(s) 424
BstSLI GKGCMC 2 cut(s) 545, 1091
BstUI CGCG 4 cut(s) 152, 350, 375, 486
BstV1I GCAGC 9 cut(s) 250, 262, 265, 277, 465, 479, 482, 823, 853
BstV2I GAAGAC 2 cut(s) 207, 1171
BstX2I RGATCY 1 cut(s) 1000
BstXI CCANNNNNNTGG 1 cut(s) 805
BstYI RGATCY 1 cut(s) 1000
BsuRI GGCC 2 cut(s) 809, 1089
BtgI CCRYGG 1 cut(s) 178
BtgZI GCGATG 1 cut(s) 467
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 5 cut(s) 127, 188, 402, 571, 684
BtsI GCAGTG 2 cut(s) 749, 768
BtsIMutI CAGTG 3 cut(s) 749, 768, 1017
Cac8I GCNNGC 7 cut(s) 53, 75, 90, 348, 373, 525, 841
CfoI GCGC 4 cut(s) 154, 488, 584, 742
Cfr13I GGNCC 3 cut(s) 1087, 1088, 1163
CseI GACGC 1 cut(s) 751
Csp6I GTAC 4 cut(s) 505, 608, 714, 1030
CviQI GTAC 4 cut(s) 505, 608, 714, 1030
DdeI CTNAG 1 cut(s) 1194
DpnI GATC 5 cut(s) 119, 258, 378, 861, 1002
DpnII GATC 5 cut(s) 117, 256, 376, 859, 1000
DraIII CACNNNGTG 1 cut(s) 1038
EaeI YGGCCR 1 cut(s) 807
EciI GGCGGA 1 cut(s) 1038
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 178
Eco24I GRGCYC 2 cut(s) 75, 1091
Eco47I GGWCC 1 cut(s) 1163
Eco53kI GAGCTC 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 3 cut(s) 57, 247, 507
EcoT14I CCWWGG 1 cut(s) 178
EcoT22I ATGCAT 1 cut(s) 556
EcoT38I GRGCYC 2 cut(s) 75, 1091
ErhI CCWWGG 1 cut(s) 178
FaqI GGGAC 1 cut(s) 761
FauI CCCGC 1 cut(s) 380
FbaI TGATCA 1 cut(s) 117
FokI GGATG 5 cut(s) 114, 195, 389, 578, 671
FriOI GRGCYC 2 cut(s) 75, 1091
FspBI CTAG 1 cut(s) 1070
FspI TGCGCA 1 cut(s) 741
GlaI GCGC 4 cut(s) 153, 487, 583, 741
GsuI CTGGAG 2 cut(s) 41, 632
HaeIII GGCC 2 cut(s) 809, 1089
HapII CCGG 6 cut(s) 137, 197, 384, 414, 435, 720
HgaI GACGC 1 cut(s) 751
HhaI GCGC 4 cut(s) 154, 488, 584, 742
Hin6I GCGC 4 cut(s) 152, 486, 582, 740
HinP1I GCGC 4 cut(s) 152, 486, 582, 740
HincII GTYRAC 2 cut(s) 625, 829
HindII GTYRAC 2 cut(s) 625, 829
HinfI GANTC 3 cut(s) 170, 317, 961
HpaII CCGG 6 cut(s) 137, 197, 384, 414, 435, 720
HphI GGTGA 2 cut(s) 371, 1151
Hpy166II GTNNAC 7 cut(s) 9, 333, 625, 716, 829, 1032, 1142
Hpy188I TCNGA 7 cut(s) 169, 175, 256, 316, 954, 1005, 1114
Hpy188III TCNNGA 3 cut(s) 307, 395, 688
Hpy8I GTNNAC 7 cut(s) 9, 333, 625, 716, 829, 1032, 1142
Hpy99I CGWCG 2 cut(s) 767, 1118
HpyAV CCTTC 3 cut(s) 235, 613, 787
HpyCH4III ACNGT 3 cut(s) 165, 504, 833
HpyCH4IV ACGT 2 cut(s) 141, 516
HpyCH4V TGCA 8 cut(s) 346, 481, 554, 599, 632, 684, 869, 977
HpyF3I CTNAG 1 cut(s) 1194
HpySE526I ACGT 2 cut(s) 141, 516
HspAI GCGC 4 cut(s) 152, 486, 582, 740
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 5 cut(s) 117, 256, 376, 859, 1000
LmnI GCTCC 6 cut(s) 30, 60, 257, 654, 941, 1076
Lsp1109I GCAGC 9 cut(s) 250, 262, 265, 277, 465, 479, 482, 823, 853
LweI GCATC 5 cut(s) 226, 411, 490, 693, 986
MaeI CTAG 1 cut(s) 1070
MaeII ACGT 2 cut(s) 141, 516
MaeIII GTNAC 2 cut(s) 923, 1012
MalI GATC 5 cut(s) 119, 258, 378, 861, 1002
MboI GATC 5 cut(s) 117, 256, 376, 859, 1000
MboII GAAGA 2 cut(s) 212, 1171
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 1 cut(s) 1000
MhlI GDGCHC 5 cut(s) 35, 75, 147, 545, 1091
MluCI AATT 7 cut(s) 231, 297, 310, 799, 946, 981, 1126
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 4 cut(s) 139, 913, 1028, 1137
MnlI CCTC 9 cut(s) 232, 587, 607, 658, 716, 832, 965, 1019, 1115
Mph1103I ATGCAT 1 cut(s) 556
MslI CAYNNNNRTG 2 cut(s) 559, 972
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 6 cut(s) 137, 197, 384, 414, 435, 720
MspR9I CCNGG 5 cut(s) 59, 249, 384, 509, 720
MunI CAATTG 1 cut(s) 297
Mva1269I GAATGC 2 cut(s) 554, 869
MvaI CCWGG 3 cut(s) 59, 249, 509
MvnI CGCG 4 cut(s) 152, 350, 375, 486
NciI CCSGG 2 cut(s) 384, 720
NcoI CCATGG 1 cut(s) 178
NdeII GATC 5 cut(s) 117, 256, 376, 859, 1000
NlaIV GGNNCC 6 cut(s) 106, 253, 656, 1002, 1040, 1089
NmeAIII GCCGAG 1 cut(s) 558
NmuCI GTSAC 1 cut(s) 1012
NsbI TGCGCA 1 cut(s) 741
NsiI ATGCAT 1 cut(s) 556
NspI RCATGY 1 cut(s) 1020
OliI CACNNNNGTG 1 cut(s) 972
PctI GAATGC 2 cut(s) 554, 869
PfeI GAWTC 2 cut(s) 170, 961
PflMI CCANNNNNTGG 2 cut(s) 99, 1172
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
PsiI TTATAA 1 cut(s) 1155
Psp124BI GAGCTC 1 cut(s) 75
Psp6I CCWGG 3 cut(s) 57, 247, 507
PspGI CCWGG 3 cut(s) 57, 247, 507
PspN4I GGNNCC 6 cut(s) 106, 253, 656, 1002, 1040, 1089
PspOMI GGGCCC 1 cut(s) 1087
PspPI GGNCC 3 cut(s) 1087, 1088, 1163
PsrI GAACNNNNNNTAC 2 cut(s) 908, 940
PsuI RGATCY 1 cut(s) 1000
RsaI GTAC 4 cut(s) 506, 609, 715, 1031
RsaNI GTAC 4 cut(s) 505, 608, 714, 1030
RseI CAYNNNNRTG 2 cut(s) 559, 972
SacI GAGCTC 1 cut(s) 75
Sau3AI GATC 5 cut(s) 117, 256, 376, 859, 1000
Sau96I GGNCC 3 cut(s) 1087, 1088, 1163
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 5 cut(s) 59, 249, 384, 509, 720
SduI GDGCHC 5 cut(s) 35, 75, 147, 545, 1091
SfaNI GCATC 5 cut(s) 226, 411, 490, 693, 986
SfcI CTRYAG 1 cut(s) 424
SinI GGWCC 1 cut(s) 1163
SmiMI CAYNNNNRTG 2 cut(s) 559, 972
Sse9I AATT 7 cut(s) 231, 297, 310, 799, 946, 981, 1126
SsiI CCGC 6 cut(s) 288, 373, 810, 958, 1020, 1023
SspMI CTAG 1 cut(s) 1070
SstI GAGCTC 1 cut(s) 75
StyD4I CCNGG 5 cut(s) 57, 247, 382, 507, 718
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 3 cut(s) 165, 504, 833
TaiI ACGT 2 cut(s) 144, 519
TaqII GACCGA 1 cut(s) 1151
TasI AATT 7 cut(s) 231, 297, 310, 799, 946, 981, 1126
TatI WGTACW 1 cut(s) 607
TauI GCSGC 3 cut(s) 290, 812, 1023
TfiI GAWTC 2 cut(s) 170, 961
TscAI CASTG 3 cut(s) 749, 775, 1017
TseFI GTSAC 1 cut(s) 1012
TseI GCWGC 9 cut(s) 263, 275, 278, 290, 467, 470, 478, 836, 866
Tsp45I GTSAC 1 cut(s) 1012
TspDTI ATGAA 5 cut(s) 468, 510, 661, 716, 953
TspGWI ACGGA 1 cut(s) 814
TspRI CASTG 3 cut(s) 749, 775, 1017
Van91I CCANNNNNTGG 2 cut(s) 99, 1172
VpaK11BI GGWCC 1 cut(s) 1163
XapI RAATTY 1 cut(s) 310
XceI RCATGY 1 cut(s) 1020
XspI CTAG 1 cut(s) 1070
Zsp2I ATGCAT 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.