pycom13g01830

TIFY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001273
Physical Location & Seq
Forward (+)
1231776 .. 1235133
3358 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g01830.2

Sequence Viewer

Length: 1152 bp
ATGGAGAGAGATTTCTTGGGTTTGAACTCAAAGGAGCCGGTGGTTGTGGTGAAGGAGGAGACCAACAACGATGGCGGCAAAGACCCAGGGTTTGGAAGAGGTGGAGGTGCTTATTCGCCGTTTCAAAACAAGGTCTCTGCAGTTCCTCATTTTATGTCCTTCAAAGCTGCTCAAGATGATAGGACTAAAAAGATGGTACCTGATTCATTCTTGTCTTCCGTCTCCGCGGATGCACTTGGCCCTTGTCAAAAACAAACTGCATATGAAGCTCAGGATTACTTCAATCACGATAGGCAAGGTGGGACTCATTTTTCCCTGACAGCTTATCATATGCAACATGATGTGCATTCTGTGCACCGTCCTCATGATGCACAGATGATTTCAGTTACCAGTCAAGGGATTTCTGTTCCAATGAGCAATCCTTACTTGAAGAATCACTTGGCTACAACCGGTCAGAATTTTTCCACCACTACCATGAAGCACCAAATATTTGGAGGAATTCCTGTTACGGCTCCACTTTCAGCTCTTCCCGTTGCAGGTGGTTCCATTGCTGGGATCACTGAACCATGGAACAATGTCAAGATCTCTGGGTCTCCTTCACAATTGACAATCTTTTATGCTGGTACTGTGAATGTCTATGATGATATCTCCCCTGAGAAGGCTCAGGCAATGATGCTTTTGGCCGGAAACAGTTGTACTAACTCCTCTAATGCTGCACAACCCAAATCTCAAGTGCCTAGTGCGAAGTTGACAGCAGAAGACGGTGTTCCTGTGAATCAGGCTACAAATATACACCCACCTGCTCTTTCTAGCCCCTTATCTGTTTCTTCACATTCTGGCGCCCAGTCAGCAAGTGGGTCCACAAGCACTGATGAACTAATGGCAGCTAGAACTTCCAGACGTCCAACCAGTCCTGTTAACAAAATGGAGGCTTTCCTGTACAGCTGTTCCACAGGCTCGCAAAGCATCCTTGGTTCGGTTTTTGGAGAAGCGCAAGGAAAGGGTGATGAGTGCAGCACCATACAACTTCGACAAGAAATCACCAGAACACGGCACTCCAGAATCCAGCGGAGTGAATTTTGGCCAACAAGGAGAACAACGATGATGCTCGGTGCTAAAGCAGAAGGGATAATTATTTCAAAATTTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CCT_2 tify

Protein Analysis

384

Amino Acids

41.03

Weight (kDa)

8.28

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 527, 808
AatII GACGTC 1 cut(s) 904
Acc36I ACCTGC 2 cut(s) 527, 808
Acc65I GGTACC 1 cut(s) 196
AccB1I GGYRCC 2 cut(s) 196, 839
AccB7I CCANNNNNTGG 1 cut(s) 92
AccII CGCG 1 cut(s) 227
AciI CCGC 4 cut(s) 75, 225, 227, 1069
AclWI GGATC 1 cut(s) 563
AcoI YGGCCR 2 cut(s) 681, 1082
AcsI RAATTY 4 cut(s) 457, 498, 1076, 1142
AcyI GRCGYC 2 cut(s) 840, 901
AfaI GTAC 4 cut(s) 198, 625, 697, 941
AfiI CCNNNNNNNGG 7 cut(s) 92, 396, 536, 552, 658, 976, 1050
AgeI ACCGGT 1 cut(s) 449
AgsI TTSAA 6 cut(s) 25, 125, 163, 283, 430, 1140
AjnI CCWGG 1 cut(s) 85
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
AluBI AGCT 6 cut(s) 167, 269, 323, 524, 887, 945
AluI AGCT 6 cut(s) 167, 269, 323, 524, 887, 945
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 4 cut(s) 53, 139, 226, 597
Alw44I GTGCAC 1 cut(s) 353
AlwI GGATC 1 cut(s) 563
AoxI GGCC 3 cut(s) 238, 681, 1082
ApaLI GTGCAC 1 cut(s) 353
ApeKI GCWGC 4 cut(s) 167, 713, 884, 1014
ApoI RAATTY 4 cut(s) 457, 498, 1076, 1142
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AsiGI ACCGGT 1 cut(s) 449
Asp718I GGTACC 1 cut(s) 196
AspLEI GCGC 2 cut(s) 842, 994
AspS9I GGNCC 2 cut(s) 239, 858
AsuHPI GGTGA 3 cut(s) 61, 1016, 1033
AvaII GGWCC 1 cut(s) 858
BaeGI GKGCMC 1 cut(s) 357
BalI TGGCCA 1 cut(s) 1084
BanI GGYRCC 2 cut(s) 196, 839
BbsI GAAGAC 2 cut(s) 207, 765
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 4 cut(s) 154, 700, 896, 1026
BccI CCATC 2 cut(s) 65, 187
BceAI ACGGC 3 cut(s) 103, 525, 1067
BciT130I CCWGG 1 cut(s) 87
BcoDI GTCTC 4 cut(s) 53, 139, 226, 597
BfaI CTAG 3 cut(s) 738, 810, 888
BfmI CTRYAG 1 cut(s) 138
BfoI RGCGCY 1 cut(s) 843
BfuAI ACCTGC 2 cut(s) 527, 808
BglII AGATCT 1 cut(s) 582
BisI GCNGC 5 cut(s) 76, 168, 714, 885, 1015
BlsI GCNGC 5 cut(s) 77, 169, 715, 886, 1016
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 1 cut(s) 858
BmgT120I GGNCC 2 cut(s) 239, 858
BmiI GGNNCC 6 cut(s) 36, 198, 513, 544, 841, 859
BmrFI CCNGG 1 cut(s) 87
BmrI ACTGGG 1 cut(s) 838
BmsI GCATC 5 cut(s) 220, 358, 663, 975, 1095
BmuI ACTGGG 1 cut(s) 838
BpiI GAAGAC 2 cut(s) 207, 765
BpmI CTGGAG 1 cut(s) 1042
Bpu10I CCTNAGC 2 cut(s) 270, 663
BpuEI CTTGAG 2 cut(s) 156, 714
BsaHI GRCGYC 2 cut(s) 840, 901
BsaI GGTCTC 3 cut(s) 53, 139, 597
BsaJI CCNNGG 5 cut(s) 85, 86, 225, 566, 970
BsaWI WCCGGW 1 cut(s) 449
Bsc4I CCNNNNNNNGG 7 cut(s) 92, 396, 536, 552, 658, 976, 1050
Bse118I RCCGGY 2 cut(s) 37, 449
Bse1I ACTGG 3 cut(s) 390, 844, 909
Bse3DI GCAATG 2 cut(s) 546, 675
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 5 cut(s) 85, 86, 225, 566, 970
BseGI GGATG 2 cut(s) 235, 966
BseLI CCNNNNNNNGG 7 cut(s) 92, 396, 536, 552, 658, 976, 1050
BseMI GCAATG 2 cut(s) 546, 675
BseMII CTCAG 3 cut(s) 284, 645, 677
BseNI ACTGG 3 cut(s) 390, 844, 909
BseRI GAGGAG 2 cut(s) 71, 694
BseSI GKGCMC 1 cut(s) 357
BseXI GCAGC 4 cut(s) 154, 700, 896, 1026
BseYI CCCAGC 1 cut(s) 551
BsgI GTGCAG 2 cut(s) 699, 1033
Bsh1236I CGCG 1 cut(s) 227
BshFI GGCC 3 cut(s) 240, 683, 1084
BshNI GGYRCC 2 cut(s) 196, 839
BshTI ACCGGT 1 cut(s) 449
BsiHKAI GWGCWC 1 cut(s) 357
BsiSI CCGG 3 cut(s) 38, 450, 684
BslFI GGGAC 1 cut(s) 316
BslI CCNNNNNNNGG 7 cut(s) 92, 396, 536, 552, 658, 976, 1050
BsmAI GTCTC 4 cut(s) 53, 139, 226, 597
BsmBI CGTCTC 1 cut(s) 226
BsmFI GGGAC 1 cut(s) 316
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 3 cut(s) 240, 683, 1084
Bso31I GGTCTC 3 cut(s) 53, 139, 597
Bsp1286I GDGCHC 1 cut(s) 357
Bsp1407I TGTACA 1 cut(s) 939
Bsp143I GATC 2 cut(s) 555, 582
Bsp19I CCATGG 1 cut(s) 566
BspACI CCGC 4 cut(s) 75, 225, 227, 1069
BspANI GGCC 3 cut(s) 240, 683, 1084
BspCNI CTCAG 3 cut(s) 283, 646, 676
BspFNI CGCG 1 cut(s) 227
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 6 cut(s) 36, 198, 513, 544, 841, 859
BspMAI CTGCAG 1 cut(s) 142
BspMI ACCTGC 2 cut(s) 527, 808
BspPI GGATC 1 cut(s) 563
BspQI GCTCTTC 1 cut(s) 531
BspT107I GGYRCC 2 cut(s) 196, 839
BspTNI GGTCTC 3 cut(s) 53, 139, 597
BsrDI GCAATG 2 cut(s) 546, 675
BsrFI RCCGGY 2 cut(s) 37, 449
BsrGI TGTACA 1 cut(s) 939
BsrI ACTGG 3 cut(s) 390, 844, 909
BssAI RCCGGY 2 cut(s) 37, 449
BssECI CCNNGG 5 cut(s) 85, 86, 225, 566, 970
BssMI GATC 2 cut(s) 555, 582
BssNI GRCGYC 2 cut(s) 840, 901
BssT1I CCWWGG 2 cut(s) 566, 970
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 4 cut(s) 359, 628, 692, 764
Bst6I CTCTTC 2 cut(s) 91, 531
BstACI GRCGYC 2 cut(s) 840, 901
BstAPI GCANNNNNTGC 1 cut(s) 352
BstAUI TGTACA 1 cut(s) 939
BstC8I GCNNGC 1 cut(s) 959
BstDEI CTNAG 3 cut(s) 270, 654, 663
BstDSI CCRYGG 2 cut(s) 225, 566
BstF5I GGATG 2 cut(s) 235, 966
BstFNI CGCG 1 cut(s) 227
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 2 cut(s) 842, 994
BstKTI GATC 2 cut(s) 558, 585
BstMAI GTCTC 4 cut(s) 53, 139, 226, 597
BstMBI GATC 2 cut(s) 555, 582
BstMWI GCNNNNNNNGC 4 cut(s) 266, 352, 848, 963
BstNI CCWGG 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 138
BstSLI GKGCMC 1 cut(s) 357
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 4 cut(s) 154, 700, 896, 1026
BstV2I GAAGAC 2 cut(s) 207, 765
BstX2I RGATCY 1 cut(s) 582
BstXI CCANNNNNNTGG 1 cut(s) 491
BstYI RGATCY 1 cut(s) 582
BsuRI GGCC 3 cut(s) 240, 683, 1084
BtgI CCRYGG 2 cut(s) 225, 566
BtsCI GGATG 2 cut(s) 235, 966
BtsIMutI CAGTG 2 cut(s) 558, 867
BveI ACCTGC 2 cut(s) 527, 808
Cac8I GCNNGC 1 cut(s) 959
CciI TCATGA 1 cut(s) 364
CfoI GCGC 2 cut(s) 842, 994
Cfr10I RCCGGY 2 cut(s) 37, 449
Cfr13I GGNCC 2 cut(s) 239, 858
Cfr42I CCGCGG 1 cut(s) 228
Csp6I GTAC 4 cut(s) 197, 624, 696, 940
CspAI ACCGGT 1 cut(s) 449
CviAII CATG 4 cut(s) 338, 365, 475, 567
CviQI GTAC 4 cut(s) 197, 624, 696, 940
DdeI CTNAG 3 cut(s) 270, 654, 663
DinI GGCGCC 1 cut(s) 841
DpnI GATC 2 cut(s) 557, 584
DpnII GATC 2 cut(s) 555, 582
EaeI YGGCCR 2 cut(s) 681, 1082
Eam1104I CTCTTC 2 cut(s) 91, 531
EarI CTCTTC 2 cut(s) 91, 531
Eco130I CCWWGG 2 cut(s) 566, 970
Eco31I GGTCTC 3 cut(s) 53, 139, 597
Eco32I GATATC 1 cut(s) 646
Eco47I GGWCC 1 cut(s) 858
EcoRI GAATTC 1 cut(s) 498
EcoRII CCWGG 1 cut(s) 85
EcoRV GATATC 1 cut(s) 646
EcoT14I CCWWGG 2 cut(s) 566, 970
EgeI GGCGCC 1 cut(s) 841
EheI GGCGCC 1 cut(s) 841
ErhI CCWWGG 2 cut(s) 566, 970
Esp3I CGTCTC 1 cut(s) 226
FaeI CATG 4 cut(s) 341, 368, 478, 570
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FaqI GGGAC 1 cut(s) 316
FatI CATG 4 cut(s) 337, 364, 474, 566
FauNDI CATATG 2 cut(s) 262, 330
Fnu4HI GCNGC 5 cut(s) 76, 168, 714, 885, 1015
FokI GGATG 2 cut(s) 242, 953
Fsp4HI GCNGC 5 cut(s) 76, 168, 714, 885, 1015
FspBI CTAG 3 cut(s) 738, 810, 888
GlaI GCGC 2 cut(s) 841, 993
GluI GCNGC 5 cut(s) 76, 168, 714, 885, 1015
GsaI CCCAGC 1 cut(s) 555
GsuI CTGGAG 1 cut(s) 1042
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 3 cut(s) 240, 683, 1084
HapII CCGG 3 cut(s) 38, 450, 684
HhaI GCGC 2 cut(s) 842, 994
Hin1I GRCGYC 2 cut(s) 840, 901
Hin1II CATG 4 cut(s) 341, 368, 478, 570
Hin6I GCGC 2 cut(s) 840, 992
HinP1I GCGC 2 cut(s) 840, 992
HincII GTYRAC 2 cut(s) 750, 919
HindII GTYRAC 2 cut(s) 750, 919
HinfI GANTC 5 cut(s) 203, 304, 433, 775, 1062
HpaI GTTAAC 1 cut(s) 919
HpaII CCGG 3 cut(s) 38, 450, 684
HphI GGTGA 3 cut(s) 61, 1016, 1033
Hpy166II GTNNAC 4 cut(s) 355, 750, 861, 919
Hpy188I TCNGA 1 cut(s) 456
Hpy188III TCNNGA 7 cut(s) 173, 272, 287, 365, 580, 897, 1059
Hpy8I GTNNAC 4 cut(s) 355, 750, 861, 919
HpyAV CCTTC 5 cut(s) 46, 169, 606, 652, 1118
HpyCH4III ACNGT 4 cut(s) 359, 628, 692, 764
HpyCH4IV ACGT 1 cut(s) 901
HpyF10VI GCNNNNNNNGC 4 cut(s) 266, 352, 848, 963
HpyF3I CTNAG 3 cut(s) 270, 654, 663
HpySE526I ACGT 1 cut(s) 901
Hsp92I GRCGYC 2 cut(s) 840, 901
Hsp92II CATG 4 cut(s) 341, 368, 478, 570
HspAI GCGC 2 cut(s) 840, 992
KasI GGCGCC 1 cut(s) 839
KpnI GGTACC 1 cut(s) 200
KspAI GTTAAC 1 cut(s) 919
KspI CCGCGG 1 cut(s) 228
Kzo9I GATC 2 cut(s) 555, 582
LguI GCTCTTC 1 cut(s) 531
LmnI GCTCC 2 cut(s) 34, 517
Lsp1109I GCAGC 4 cut(s) 154, 700, 896, 1026
LweI GCATC 5 cut(s) 220, 358, 663, 975, 1095
MaeI CTAG 3 cut(s) 738, 810, 888
MaeII ACGT 1 cut(s) 901
MaeIII GTNAC 2 cut(s) 385, 505
MalI GATC 2 cut(s) 557, 584
MboI GATC 2 cut(s) 555, 582
MboII GAAGA 6 cut(s) 108, 207, 442, 518, 770, 819
MfeI CAATTG 1 cut(s) 602
MflI RGATCY 1 cut(s) 582
MhlI GDGCHC 1 cut(s) 357
MlsI TGGCCA 1 cut(s) 1084
MluCI AATT 6 cut(s) 457, 498, 602, 1076, 1131, 1142
MluNI TGGCCA 1 cut(s) 1084
Mly113I GGCGCC 1 cut(s) 840
MlyI GAGTC 1 cut(s) 298
MmeI TCCRAC 1 cut(s) 929
MnlI CCTC 8 cut(s) 49, 92, 98, 156, 372, 488, 715, 922
Mox20I TGGCCA 1 cut(s) 1084
MscI TGGCCA 1 cut(s) 1084
MseI TTAA 1 cut(s) 918
MslI CAYNNNNRTG 1 cut(s) 473
Msp20I TGGCCA 1 cut(s) 1084
MspA1I CMGCKG 3 cut(s) 227, 945, 1069
MspI CCGG 3 cut(s) 38, 450, 684
MspR9I CCNGG 1 cut(s) 87
MunI CAATTG 1 cut(s) 602
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 1 cut(s) 87
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 4 cut(s) 266, 352, 848, 963
NarI GGCGCC 1 cut(s) 840
NcoI CCATGG 1 cut(s) 566
NdeI CATATG 2 cut(s) 262, 330
NdeII GATC 2 cut(s) 555, 582
NlaIII CATG 4 cut(s) 341, 368, 478, 570
NlaIV GGNNCC 6 cut(s) 36, 198, 513, 544, 841, 859
PagI TCATGA 1 cut(s) 364
PaqCI CACCTGC 2 cut(s) 527, 808
PasI CCCWGGG 1 cut(s) 86
PciSI GCTCTTC 1 cut(s) 531
PctI GAATGC 1 cut(s) 346
PfeI GAWTC 4 cut(s) 203, 433, 775, 1062
PflMI CCANNNNNTGG 1 cut(s) 92
PinAI ACCGGT 1 cut(s) 449
PkrI GCNGC 5 cut(s) 77, 169, 715, 886, 1016
PleI GAGTC 1 cut(s) 298
PluTI GGCGCC 1 cut(s) 843
PpsI GAGTC 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 85
PspFI CCCAGC 1 cut(s) 551
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 6 cut(s) 36, 198, 513, 544, 841, 859
PspPI GGNCC 2 cut(s) 239, 858
PstI CTGCAG 1 cut(s) 142
PsuI RGATCY 1 cut(s) 582
PvuII CAGCTG 1 cut(s) 945
RsaI GTAC 4 cut(s) 198, 625, 697, 941
RsaNI GTAC 4 cut(s) 197, 624, 696, 940
RseI CAYNNNNRTG 1 cut(s) 473
SacII CCGCGG 1 cut(s) 228
SapI GCTCTTC 1 cut(s) 531
SaqAI TTAA 1 cut(s) 918
SatI GCNGC 5 cut(s) 76, 168, 714, 885, 1015
Sau3AI GATC 2 cut(s) 555, 582
Sau96I GGNCC 2 cut(s) 239, 858
SchI GAGTC 1 cut(s) 298
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 357
SfaNI GCATC 5 cut(s) 220, 358, 663, 975, 1095
SfcI CTRYAG 1 cut(s) 138
SfoI GGCGCC 1 cut(s) 841
Sfr303I CCGCGG 1 cut(s) 228
SgrBI CCGCGG 1 cut(s) 228
SinI GGWCC 1 cut(s) 858
SmiMI CAYNNNNRTG 1 cut(s) 473
SmlI CTYRAG 2 cut(s) 171, 729
SmoI CTYRAG 2 cut(s) 171, 729
Sse9I AATT 6 cut(s) 457, 498, 602, 1076, 1131, 1142
SsiI CCGC 4 cut(s) 75, 225, 227, 1069
SspDI GGCGCC 1 cut(s) 839
SspI AATATT 1 cut(s) 489
SspMI CTAG 3 cut(s) 738, 810, 888
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 2 cut(s) 566, 970
TaaI ACNGT 4 cut(s) 359, 628, 692, 764
TaiI ACGT 1 cut(s) 904
TaqI TCGA 1 cut(s) 1030
TasI AATT 6 cut(s) 457, 498, 602, 1076, 1131, 1142
TatI WGTACW 2 cut(s) 695, 939
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 4 cut(s) 203, 433, 775, 1062
Tru1I TTAA 1 cut(s) 918
Tru9I TTAA 1 cut(s) 918
TscAI CASTG 2 cut(s) 565, 874
TseI GCWGC 4 cut(s) 167, 713, 884, 1014
TspDTI ATGAA 4 cut(s) 195, 279, 491, 888
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 2 cut(s) 565, 874
Van91I CCANNNNNTGG 1 cut(s) 92
VneI GTGCAC 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 858
XapI RAATTY 4 cut(s) 457, 498, 1076, 1142
XcmI CCANNNNNNNNNTGG 1 cut(s) 851
XspI CTAG 3 cut(s) 738, 810, 888
ZraI GACGTC 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.