pycom16g01860

TIFY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001273
Physical Location & Seq
Forward (+)
1140577 .. 1143504
2928 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g01860.7

Sequence Viewer

Length: 1089 bp
ATGGAGAGAGATTTCTTGGGTTTGAACTCAAGGGAGCCGGTGGTTGTGGTGAAGGAGGAGACCAACAACGATGGCGGCAAAGACCCAGGGTATGGAAGAGGTGGAAGGGCACATTGGCCCTTTCTAAACAACGTCTCTGCAGTTCCTCAGTTTATGTCCTTCAAAGCTGCTCAAGATGATAGGACTAAAAAAATGGTACCTGATTCATTCTTGTCTTCCATCTCTGCGGATGCATTTGACCTTTGTCGAAAACAAACTACATATGAATCTCAGAAGAACTTCAATCACGATAGGCAAGGTGGGATTCATTTTTCTCTGACAGCTTATCCTAGGCAACGTGATATGCATTCTGTGCACCGTCCTCATGATACGAAGATGATTTCAGTCACCAGTCAAGGGATTTCTGTTCTAATGAGCAACCCTTACTTGAAGAATCACTTTACTACTACTGGTCAGAATTTTTCCACCACTACCATGAAGCAGACGGCTCCAATTTCAGCTCTTCCCGTTACAGGTGGTTCCATTGCTGGGATCACTGAGCCGTGGAACAATGTCAAGACCTCTGGGTCTCCTTCACAATTGACAATCTTTTACGCTGGTACTGTGAATGTCTATGATGATATCTCCCCTGAGAAGGCTCAGGCACTGATGCTTTTGGCTAGAAACAGTTGTTCTAACTCCTCTAGTGCTGCACAGCCCAAAGCTCAAGCACCGAGTGCAAAGTTGGCGGTGGAAGACGGTGTTCCTGTGAATCAGCCTACAAATATACCCCCACCTGCTCTTTCTAGCCCCTTATCTGTTTCTTCACATACTGGTGCCCAGTCAGTAAGTGGGTCCACCAGCACTGATGAACTAATGTCAGCTAGGACGACCGGACGTCCAACCAGTCCTGTTAACAAAATGGAGGCTCCTTCTATAATTCCTTCTGGTGTTCCACAGGCTCGCAAAGCTTCCTTGGCTCGATTTTTAGAGAAGCGCAAGGAAAGGGTGATGAGTGCAGCACCATACAATTTCGAAAAGAAATCACCGGAATATGGCACTCCGAAATCCAACGGAGTGAATTTTGGTCAACAAGGAGAACAACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CCT_2 tify

Protein Analysis

363

Amino Acids

39.15

Weight (kDa)

9.46

Isoelectric Point (pI)

51.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 784
AasI GACNNNNNNGTC 1 cut(s) 565
AatII GACGTC 1 cut(s) 880
Acc36I ACCTGC 1 cut(s) 784
Acc65I GGTACC 1 cut(s) 196
AccB1I GGYRCC 2 cut(s) 196, 815
AccB7I CCANNNNNTGG 1 cut(s) 92
AciI CCGC 3 cut(s) 75, 227, 728
AclWI GGATC 1 cut(s) 539
AcsI RAATTY 2 cut(s) 457, 1060
AcyI GRCGYC 1 cut(s) 877
AdeI CACNNNGTG 1 cut(s) 716
AfaI GTAC 2 cut(s) 198, 601
AfiI CCNNNNNNNGG 6 cut(s) 92, 396, 512, 528, 634, 1034
AgsI TTSAA 4 cut(s) 25, 163, 283, 430
AhdI GACNNNNNGTC 1 cut(s) 876
AjnI CCWGG 1 cut(s) 85
AjuI GAANNNNNNNTTGG 2 cut(s) 97, 129
AluBI AGCT 6 cut(s) 167, 323, 500, 704, 863, 950
AluI AGCT 6 cut(s) 167, 323, 500, 704, 863, 950
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 3 cut(s) 53, 139, 573
Alw44I GTGCAC 1 cut(s) 353
AlwI GGATC 1 cut(s) 539
AlwNI CAGNNNCTG 1 cut(s) 646
AoxI GGCC 1 cut(s) 116
ApaLI GTGCAC 1 cut(s) 353
ApeKI GCWGC 3 cut(s) 167, 689, 998
ApoI RAATTY 2 cut(s) 457, 1060
Asp700I GAANNNNTTC 1 cut(s) 278
Asp718I GGTACC 1 cut(s) 196
AspA2I CCTAGG 1 cut(s) 329
AspLEI GCGC 1 cut(s) 978
AspS9I GGNCC 2 cut(s) 117, 834
AsuHPI GGTGA 4 cut(s) 61, 379, 1000, 1017
AsuII TTCGAA 1 cut(s) 1014
AvaII GGWCC 1 cut(s) 834
AvrII CCTAGG 1 cut(s) 329
BaeGI GKGCMC 3 cut(s) 112, 357, 820
BanI GGYRCC 2 cut(s) 196, 815
BbsI GAAGAC 2 cut(s) 207, 741
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 3 cut(s) 154, 676, 1010
BccI CCATC 2 cut(s) 65, 227
BceAI ACGGC 2 cut(s) 501, 526
BciT130I CCWGG 1 cut(s) 87
BcoDI GTCTC 3 cut(s) 53, 139, 573
BfaI CTAG 5 cut(s) 330, 660, 684, 786, 864
BfmI CTRYAG 1 cut(s) 138
BfuAI ACCTGC 1 cut(s) 784
BisI GCNGC 4 cut(s) 76, 168, 690, 999
BlnI CCTAGG 1 cut(s) 329
BlsI GCNGC 4 cut(s) 77, 169, 691, 1000
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 1 cut(s) 834
BmeRI GACNNNNNGTC 1 cut(s) 876
BmgT120I GGNCC 2 cut(s) 117, 834
BmiI GGNNCC 7 cut(s) 36, 198, 489, 520, 817, 835, 909
BmrFI CCNGG 1 cut(s) 87
BmrI ACTGGG 1 cut(s) 814
BmsI GCATC 2 cut(s) 220, 639
BmuI ACTGGG 1 cut(s) 814
BoxI GACNNNNGTC 1 cut(s) 243
BpiI GAAGAC 2 cut(s) 207, 741
Bpu10I CCTNAGC 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 1014
BpuEI CTTGAG 3 cut(s) 13, 156, 690
BsaHI GRCGYC 1 cut(s) 877
BsaI GGTCTC 2 cut(s) 53, 573
BsaJI CCNNGG 5 cut(s) 85, 86, 329, 542, 954
BsaWI WCCGGW 2 cut(s) 872, 1027
Bsc4I CCNNNNNNNGG 6 cut(s) 92, 396, 512, 528, 634, 1034
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 5 cut(s) 390, 454, 817, 820, 885
Bse3DI GCAATG 1 cut(s) 522
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 5 cut(s) 85, 86, 329, 542, 954
BseGI GGATG 1 cut(s) 235
BseLI CCNNNNNNNGG 6 cut(s) 92, 396, 512, 528, 634, 1034
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 5 cut(s) 161, 284, 528, 621, 653
BseNI ACTGG 5 cut(s) 390, 454, 817, 820, 885
BseRI GAGGAG 2 cut(s) 71, 670
BseSI GKGCMC 3 cut(s) 112, 357, 820
BseXI GCAGC 3 cut(s) 154, 676, 1010
BseYI CCCAGC 1 cut(s) 527
BsgI GTGCAG 2 cut(s) 675, 1017
Bsh1285I CGRYCG 1 cut(s) 873
BshFI GGCC 1 cut(s) 118
BshNI GGYRCC 2 cut(s) 196, 815
BsiEI CGRYCG 1 cut(s) 873
BsiHKAI GWGCWC 1 cut(s) 357
BsiSI CCGG 3 cut(s) 38, 873, 1028
BslI CCNNNNNNNGG 6 cut(s) 92, 396, 512, 528, 634, 1034
BsmAI GTCTC 3 cut(s) 53, 139, 573
BsmBI CGTCTC 1 cut(s) 139
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 1 cut(s) 118
Bso31I GGTCTC 2 cut(s) 53, 573
Bsp119I TTCGAA 1 cut(s) 1014
Bsp1286I GDGCHC 3 cut(s) 112, 357, 820
Bsp143I GATC 1 cut(s) 531
BspACI CCGC 3 cut(s) 75, 227, 728
BspANI GGCC 1 cut(s) 118
BspCNI CTCAG 5 cut(s) 160, 283, 529, 622, 652
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 7 cut(s) 36, 198, 489, 520, 817, 835, 909
BspMAI CTGCAG 1 cut(s) 142
BspMI ACCTGC 1 cut(s) 784
BspPI GGATC 1 cut(s) 539
BspQI GCTCTTC 1 cut(s) 507
BspT104I TTCGAA 1 cut(s) 1014
BspT107I GGYRCC 2 cut(s) 196, 815
BspTNI GGTCTC 2 cut(s) 53, 573
BsrDI GCAATG 1 cut(s) 522
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 5 cut(s) 390, 454, 817, 820, 885
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 5 cut(s) 85, 86, 329, 542, 954
BssMI GATC 1 cut(s) 531
BssNI GRCGYC 1 cut(s) 877
BssT1I CCWWGG 2 cut(s) 329, 954
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 4 cut(s) 359, 604, 668, 740
Bst6I CTCTTC 2 cut(s) 91, 507
BstACI GRCGYC 1 cut(s) 877
BstAPI GCANNNNNTGC 2 cut(s) 352, 716
BstBI TTCGAA 1 cut(s) 1014
BstC8I GCNNGC 1 cut(s) 943
BstDEI CTNAG 5 cut(s) 147, 270, 537, 630, 639
BstDSI CCRYGG 1 cut(s) 542
BstF5I GGATG 1 cut(s) 235
BstHHI GCGC 1 cut(s) 978
BstKTI GATC 1 cut(s) 534
BstMAI GTCTC 3 cut(s) 53, 139, 573
BstMBI GATC 1 cut(s) 531
BstMCI CGRYCG 1 cut(s) 873
BstMWI GCNNNNNNNGC 5 cut(s) 352, 716, 725, 947, 956
BstNI CCWGG 1 cut(s) 87
BstPAI GACNNNNGTC 1 cut(s) 243
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 1 cut(s) 138
BstSLI GKGCMC 3 cut(s) 112, 357, 820
BstV1I GCAGC 3 cut(s) 154, 676, 1010
BstV2I GAAGAC 2 cut(s) 207, 741
BsuRI GGCC 1 cut(s) 118
BtgI CCRYGG 1 cut(s) 542
BtsCI GGATG 1 cut(s) 235
BtsIMutI CAGTG 3 cut(s) 534, 644, 843
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 1 cut(s) 943
CaiI CAGNNNCTG 1 cut(s) 646
CciI TCATGA 1 cut(s) 364
CfoI GCGC 1 cut(s) 978
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 2 cut(s) 117, 834
Csp6I GTAC 2 cut(s) 197, 600
CviAII CATG 2 cut(s) 365, 475
CviQI GTAC 2 cut(s) 197, 600
DdeI CTNAG 5 cut(s) 147, 270, 537, 630, 639
DpnI GATC 1 cut(s) 533
DpnII GATC 1 cut(s) 531
DraIII CACNNNGTG 1 cut(s) 716
DrdI GACNNNNNNGTC 1 cut(s) 565
DriI GACNNNNNGTC 1 cut(s) 876
DseDI GACNNNNNNGTC 1 cut(s) 565
Eam1104I CTCTTC 2 cut(s) 91, 507
Eam1105I GACNNNNNGTC 1 cut(s) 876
EarI CTCTTC 2 cut(s) 91, 507
Eco130I CCWWGG 2 cut(s) 329, 954
Eco31I GGTCTC 2 cut(s) 53, 573
Eco32I GATATC 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 834
EcoRII CCWGG 1 cut(s) 85
EcoRV GATATC 1 cut(s) 622
EcoT14I CCWWGG 2 cut(s) 329, 954
EcoT22I ATGCAT 2 cut(s) 235, 348
ErhI CCWWGG 2 cut(s) 329, 954
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 2 cut(s) 368, 478
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FatI CATG 2 cut(s) 364, 474
FauNDI CATATG 1 cut(s) 262
Fnu4HI GCNGC 4 cut(s) 76, 168, 690, 999
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 4 cut(s) 76, 168, 690, 999
FspBI CTAG 5 cut(s) 330, 660, 684, 786, 864
GlaI GCGC 1 cut(s) 977
GluI GCNGC 4 cut(s) 76, 168, 690, 999
GsaI CCCAGC 1 cut(s) 531
HaeIII GGCC 1 cut(s) 118
HapII CCGG 3 cut(s) 38, 873, 1028
HhaI GCGC 1 cut(s) 978
Hin1I GRCGYC 1 cut(s) 877
Hin1II CATG 2 cut(s) 368, 478
Hin6I GCGC 1 cut(s) 976
HinP1I GCGC 1 cut(s) 976
HincII GTYRAC 2 cut(s) 895, 1070
HindII GTYRAC 2 cut(s) 895, 1070
HindIII AAGCTT 1 cut(s) 948
HinfI GANTC 5 cut(s) 203, 266, 304, 433, 751
HpaI GTTAAC 1 cut(s) 895
HpaII CCGG 3 cut(s) 38, 873, 1028
HphI GGTGA 4 cut(s) 61, 379, 1000, 1017
Hpy166II GTNNAC 4 cut(s) 355, 837, 895, 1070
Hpy188I TCNGA 4 cut(s) 273, 318, 456, 1044
Hpy188III TCNNGA 4 cut(s) 173, 287, 365, 556
Hpy8I GTNNAC 4 cut(s) 355, 837, 895, 1070
HpyAV CCTTC 7 cut(s) 46, 99, 169, 582, 628, 921, 933
HpyCH4III ACNGT 4 cut(s) 359, 604, 668, 740
HpyCH4IV ACGT 3 cut(s) 132, 337, 877
HpyCH4V TGCA 7 cut(s) 140, 233, 346, 355, 692, 719, 998
HpyF10VI GCNNNNNNNGC 5 cut(s) 352, 716, 725, 947, 956
HpyF3I CTNAG 5 cut(s) 147, 270, 537, 630, 639
HpySE526I ACGT 3 cut(s) 132, 337, 877
Hsp92I GRCGYC 1 cut(s) 877
Hsp92II CATG 2 cut(s) 368, 478
HspAI GCGC 1 cut(s) 976
KpnI GGTACC 1 cut(s) 200
KspAI GTTAAC 1 cut(s) 895
Kzo9I GATC 1 cut(s) 531
LguI GCTCTTC 1 cut(s) 507
LmnI GCTCC 3 cut(s) 34, 493, 913
Lsp1109I GCAGC 3 cut(s) 154, 676, 1010
LweI GCATC 2 cut(s) 220, 639
MaeI CTAG 5 cut(s) 330, 660, 684, 786, 864
MaeII ACGT 3 cut(s) 132, 337, 877
MaeIII GTNAC 2 cut(s) 385, 508
MalI GATC 1 cut(s) 533
MboI GATC 1 cut(s) 531
MboII GAAGA 8 cut(s) 108, 207, 286, 385, 442, 494, 746, 795
MfeI CAATTG 1 cut(s) 578
MhlI GDGCHC 3 cut(s) 112, 357, 820
MluCI AATT 6 cut(s) 457, 492, 578, 918, 1009, 1060
MmeI TCCRAC 2 cut(s) 905, 1074
MnlI CCTC 7 cut(s) 49, 92, 156, 372, 571, 691, 898
Mph1103I ATGCAT 2 cut(s) 235, 348
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 1 cut(s) 894
MslI CAYNNNNRTG 2 cut(s) 473, 813
MspI CCGG 3 cut(s) 38, 873, 1028
MspR9I CCNGG 1 cut(s) 87
MunI CAATTG 1 cut(s) 578
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 5 cut(s) 352, 716, 725, 947, 956
NdeI CATATG 1 cut(s) 262
NdeII GATC 1 cut(s) 531
NlaIII CATG 2 cut(s) 368, 478
NlaIV GGNNCC 7 cut(s) 36, 198, 489, 520, 817, 835, 909
NmuCI GTSAC 1 cut(s) 385
NsiI ATGCAT 2 cut(s) 235, 348
NspV TTCGAA 1 cut(s) 1014
PagI TCATGA 1 cut(s) 364
PaqCI CACCTGC 1 cut(s) 784
PasI CCCWGGG 1 cut(s) 86
PciSI GCTCTTC 1 cut(s) 507
PctI GAATGC 1 cut(s) 346
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 5 cut(s) 203, 266, 304, 433, 751
PflMI CCANNNNNTGG 1 cut(s) 92
PkrI GCNGC 4 cut(s) 77, 169, 691, 1000
PshAI GACNNNNGTC 1 cut(s) 243
Psp6I CCWGG 1 cut(s) 85
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 7 cut(s) 36, 198, 489, 520, 817, 835, 909
PspPI GGNCC 2 cut(s) 117, 834
PstI CTGCAG 1 cut(s) 142
PstNI CAGNNNCTG 1 cut(s) 646
RsaI GTAC 2 cut(s) 198, 601
RsaNI GTAC 2 cut(s) 197, 600
RseI CAYNNNNRTG 2 cut(s) 473, 813
SapI GCTCTTC 1 cut(s) 507
SaqAI TTAA 1 cut(s) 894
SatI GCNGC 4 cut(s) 76, 168, 690, 999
Sau3AI GATC 1 cut(s) 531
Sau96I GGNCC 2 cut(s) 117, 834
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 3 cut(s) 112, 357, 820
SfaNI GCATC 2 cut(s) 220, 639
SfcI CTRYAG 1 cut(s) 138
SfuI TTCGAA 1 cut(s) 1014
SinI GGWCC 1 cut(s) 834
SmiMI CAYNNNNRTG 2 cut(s) 473, 813
SmlI CTYRAG 3 cut(s) 28, 171, 705
SmoI CTYRAG 3 cut(s) 28, 171, 705
Sse9I AATT 6 cut(s) 457, 492, 578, 918, 1009, 1060
SsiI CCGC 3 cut(s) 75, 227, 728
SspMI CTAG 5 cut(s) 330, 660, 684, 786, 864
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 2 cut(s) 329, 954
TaaI ACNGT 4 cut(s) 359, 604, 668, 740
TaiI ACGT 3 cut(s) 135, 340, 880
TaqI TCGA 3 cut(s) 247, 961, 1014
TasI AATT 6 cut(s) 457, 492, 578, 918, 1009, 1060
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 5 cut(s) 203, 266, 304, 433, 751
Tru1I TTAA 1 cut(s) 894
Tru9I TTAA 1 cut(s) 894
TscAI CASTG 3 cut(s) 541, 651, 850
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 3 cut(s) 167, 689, 998
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 5 cut(s) 195, 279, 296, 491, 864
TspGWI ACGGA 1 cut(s) 1068
TspRI CASTG 3 cut(s) 541, 651, 850
Van91I CCANNNNNTGG 1 cut(s) 92
VneI GTGCAC 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 834
XapI RAATTY 2 cut(s) 457, 1060
XcmI CCANNNNNNNNNTGG 1 cut(s) 827
XmaJI CCTAGG 1 cut(s) 329
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 5 cut(s) 330, 660, 684, 786, 864
ZraI GACGTC 1 cut(s) 878
Zsp2I ATGCAT 2 cut(s) 235, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.