pycom15g02070

Bifunctional dihydroflavonol 4-reductase flavanone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002832
Physical Location & Seq
Forward (+)
1227711 .. 1229380
1670 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 510 bp
ATGGGATCCGAGTCCGAATCCGTTTGTGTCACCGGCGCGTCCGGTTTCATCGGCTCTTGGCTCGTCATGAGACTCCTAGAGCACGGGTACACCGTCCGCGCCACCGTACGTGACCCCACGAATCAGAAGAAGGTGAAGCATCTGTTGGACTTGCCGAAGGCGGAGACGCACCTGACACTGTGGAAGGCGGACTTGGCGGATGAGGGAAGCTTTGATGAAGCCATACAAGGCTGCAGCGGAGTGTTCCATGTTGCTACACCTATGGACTTTGAATCGAAGGACCCCGAGAATGAAGTGATCAAGCCAACAATCAATGGACTGTTGGACATCCTGAAGGCATGCCAGAAAGCAAAAACAGTTCGAAAGCTTGTGTTCACATCCTCAGCAGGAACTGTGAATGTGGAGGAGCACCAAAAGCCGGTCTACGACGAGTCCAACTGGAGTGACGTTGAATTTTGCCGCTCTGTCAAGATGACTGGTTGGGTACTTCGTCTCCAAGACTCTAGCTGA

Protein Analysis

170

Amino Acids

18.74

Weight (kDa)

5.53

Isoelectric Point (pI)

27.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 143 1.7e-13 NmrA-like family
Epimerase PF01370 8 - 140 9.3e-19 NAD dependent epimerase/dehydratase family
KR PF08659 8 - 138 7.9e-07 KR domain
3Beta_HSD PF01073 10 - 144 8.1e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 129 4.3e-15 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 139 2.5e-09 Male sterility protein
NAD_binding_10 PF13460 12 - 137 1.7e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 462
AccI GTMKAC 1 cut(s) 423
AccII CGCG 2 cut(s) 38, 99
AciI CCGC 6 cut(s) 97, 161, 188, 197, 237, 460
AclWI GGATC 1 cut(s) 13
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 353
AfaI GTAC 3 cut(s) 89, 108, 486
AfiI CCNNNNNNNGG 1 cut(s) 418
AgsI TTSAA 2 cut(s) 272, 452
AjuI GAANNNNNNNTTGG 4 cut(s) 128, 160, 176, 208
AluBI AGCT 3 cut(s) 210, 367, 507
AluI AGCT 3 cut(s) 210, 367, 507
Alw21I GWGCWC 2 cut(s) 84, 411
Alw26I GTCTC 3 cut(s) 64, 158, 497
AlwI GGATC 1 cut(s) 13
AlwNI CAGNNNCTG 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 284
ApeKI GCWGC 2 cut(s) 231, 234
ApoI RAATTY 1 cut(s) 452
AspLEI GCGC 2 cut(s) 38, 101
AspS9I GGNCC 1 cut(s) 280
AsuHPI GGTGA 2 cut(s) 22, 145
AsuII TTCGAA 1 cut(s) 361
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 1 cut(s) 280
BamHI GGATCC 1 cut(s) 5
Bbv12I GWGCWC 2 cut(s) 84, 411
BbvCI CCTCAGC 1 cut(s) 382
BbvI GCAGC 2 cut(s) 218, 246
BclI TGATCA 1 cut(s) 297
BcoDI GTCTC 3 cut(s) 64, 158, 497
BfaI CTAG 2 cut(s) 77, 504
BfmI CTRYAG 1 cut(s) 232
BisI GCNGC 3 cut(s) 232, 235, 460
BlsI GCNGC 3 cut(s) 233, 236, 461
Bme18I GGWCC 1 cut(s) 280
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 2 cut(s) 7, 282
BmsI GCATC 1 cut(s) 148
BpmI CTGGAG 1 cut(s) 460
Bpu10I CCTNAGC 1 cut(s) 382
Bpu14I TTCGAA 1 cut(s) 361
BsaAI YACGTR 1 cut(s) 110
BsaWI WCCGGW 1 cut(s) 41
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 1 cut(s) 418
Bse118I RCCGGY 2 cut(s) 32, 418
Bse1I ACTGG 2 cut(s) 443, 481
BseGI GGATG 3 cut(s) 205, 327, 377
BseLI CCNNNNNNNGG 1 cut(s) 418
BseMII CTCAG 1 cut(s) 396
BseNI ACTGG 2 cut(s) 443, 481
BseRI GAGGAG 1 cut(s) 419
BseXI GCAGC 2 cut(s) 218, 246
Bsh1236I CGCG 2 cut(s) 38, 99
BsiHKAI GWGCWC 2 cut(s) 84, 411
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 3 cut(s) 33, 42, 419
BsiWI CGTACG 1 cut(s) 106
BslI CCNNNNNNNGG 1 cut(s) 418
BsmAI GTCTC 3 cut(s) 64, 158, 497
BsmBI CGTCTC 2 cut(s) 158, 497
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 361
Bsp1286I GDGCHC 2 cut(s) 84, 411
Bsp143I GATC 2 cut(s) 5, 297
BspACI CCGC 6 cut(s) 97, 161, 188, 197, 237, 460
BspCNI CTCAG 1 cut(s) 395
BspFNI CGCG 2 cut(s) 38, 99
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 2 cut(s) 7, 282
BspMAI CTGCAG 1 cut(s) 236
BspPI GGATC 1 cut(s) 13
BspT104I TTCGAA 1 cut(s) 361
BsrBI CCGCTC 1 cut(s) 462
BsrFI RCCGGY 2 cut(s) 32, 418
BsrI ACTGG 2 cut(s) 443, 481
BssAI RCCGGY 2 cut(s) 32, 418
BssMI GATC 2 cut(s) 5, 297
Bst4CI ACNGT 6 cut(s) 94, 106, 180, 321, 358, 394
BstBAI YACGTR 1 cut(s) 110
BstBI TTCGAA 1 cut(s) 361
BstC8I GCNNGC 1 cut(s) 340
BstDEI CTNAG 1 cut(s) 382
BstF5I GGATG 3 cut(s) 205, 327, 377
BstFNI CGCG 2 cut(s) 38, 99
BstHHI GCGC 2 cut(s) 38, 101
BstKTI GATC 2 cut(s) 8, 300
BstMAI GTCTC 3 cut(s) 64, 158, 497
BstMBI GATC 2 cut(s) 5, 297
BstMWI GCNNNNNNNGC 2 cut(s) 194, 415
BstNSI RCATGY 1 cut(s) 342
BstSFI CTRYAG 1 cut(s) 232
BstUI CGCG 2 cut(s) 38, 99
BstV1I GCAGC 2 cut(s) 218, 246
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
BtsCI GGATG 3 cut(s) 205, 327, 377
BtsIMutI CAGTG 1 cut(s) 176
Cac8I GCNNGC 1 cut(s) 340
CaiI CAGNNNCTG 1 cut(s) 392
CciI TCATGA 1 cut(s) 66
CfoI GCGC 2 cut(s) 38, 101
Cfr10I RCCGGY 2 cut(s) 32, 418
Cfr13I GGNCC 1 cut(s) 280
CseI GACGC 2 cut(s) 27, 175
Csp6I GTAC 3 cut(s) 88, 107, 485
CviAII CATG 3 cut(s) 67, 248, 339
CviJI RGCY 9 cut(s) 54, 61, 210, 221, 231, 304, 367, 418, 507
CviKI_1 RGCY 9 cut(s) 54, 61, 210, 221, 231, 304, 367, 418, 507
CviQI GTAC 3 cut(s) 88, 107, 485
DdeI CTNAG 1 cut(s) 382
DpnI GATC 2 cut(s) 7, 299
DpnII GATC 2 cut(s) 5, 297
EciI GGCGGA 3 cut(s) 176, 203, 212
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 353
Eco88I CYCGRG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 280
Esp3I CGTCTC 2 cut(s) 158, 497
FaeI CATG 3 cut(s) 70, 251, 342
FaiI YATR 5 cut(s) 68, 224, 249, 263, 340
FalI AAGNNNNNCTT 2 cut(s) 176, 208
FatI CATG 3 cut(s) 66, 247, 338
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 3 cut(s) 232, 235, 460
FokI GGATG 3 cut(s) 212, 314, 364
Fsp4HI GCNGC 3 cut(s) 232, 235, 460
FspBI CTAG 2 cut(s) 77, 504
GlaI GCGC 2 cut(s) 37, 100
GluI GCNGC 3 cut(s) 232, 235, 460
GsuI CTGGAG 1 cut(s) 460
HapII CCGG 3 cut(s) 33, 42, 419
HgaI GACGC 2 cut(s) 27, 175
HhaI GCGC 2 cut(s) 38, 101
Hin1II CATG 3 cut(s) 70, 251, 342
Hin6I GCGC 2 cut(s) 36, 99
HinP1I GCGC 2 cut(s) 36, 99
HindIII AAGCTT 2 cut(s) 208, 365
HinfI GANTC 7 cut(s) 11, 17, 72, 121, 272, 431, 500
HpaII CCGG 3 cut(s) 33, 42, 419
HphI GGTGA 2 cut(s) 22, 145
Hpy166II GTNNAC 3 cut(s) 90, 375, 424
Hpy188I TCNGA 3 cut(s) 10, 16, 126
Hpy188III TCNNGA 3 cut(s) 67, 331, 469
Hpy8I GTNNAC 3 cut(s) 90, 375, 424
Hpy99I CGWCG 1 cut(s) 431
HpyAV CCTTC 5 cut(s) 124, 151, 178, 271, 328
HpyCH4III ACNGT 6 cut(s) 94, 106, 180, 321, 358, 394
HpyCH4IV ACGT 2 cut(s) 109, 447
HpyCH4V TGCA 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 415
HpyF3I CTNAG 1 cut(s) 382
HpySE526I ACGT 2 cut(s) 109, 447
Hsp92II CATG 3 cut(s) 70, 251, 342
HspAI GCGC 2 cut(s) 36, 99
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 2 cut(s) 5, 297
LmnI GCTCC 1 cut(s) 406
LpnPI CCDG 9 cut(s) 46, 55, 185, 344, 356, 372, 424, 432, 462
Lsp1109I GCAGC 2 cut(s) 218, 246
LweI GCATC 1 cut(s) 148
MaeI CTAG 2 cut(s) 77, 504
MaeII ACGT 2 cut(s) 109, 447
MaeIII GTNAC 3 cut(s) 28, 110, 443
MalI GATC 2 cut(s) 7, 299
MbiI CCGCTC 1 cut(s) 462
MboI GATC 2 cut(s) 5, 297
MboII GAAGA 1 cut(s) 139
MflI RGATCY 1 cut(s) 5
MhlI GDGCHC 2 cut(s) 84, 411
MluCI AATT 1 cut(s) 452
MlyI GAGTC 4 cut(s) 20, 66, 440, 494
MmeI TCCRAC 3 cut(s) 126, 303, 459
MnlI CCTC 3 cut(s) 196, 391, 397
MspA1I CMGCKG 1 cut(s) 237
MspI CCGG 3 cut(s) 33, 42, 419
MvnI CGCG 2 cut(s) 38, 99
MwoI GCNNNNNNNGC 2 cut(s) 194, 415
NdeII GATC 2 cut(s) 5, 297
NlaIII CATG 3 cut(s) 70, 251, 342
NlaIV GGNNCC 2 cut(s) 7, 282
NmuCI GTSAC 3 cut(s) 28, 110, 443
NspI RCATGY 1 cut(s) 342
NspV TTCGAA 1 cut(s) 361
PaeI GCATGC 1 cut(s) 342
PagI TCATGA 1 cut(s) 66
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PfeI GAWTC 3 cut(s) 17, 121, 272
Pfl23II CGTACG 1 cut(s) 106
PkrI GCNGC 3 cut(s) 233, 236, 461
PleI GAGTC 4 cut(s) 19, 66, 439, 494
PpsI GAGTC 4 cut(s) 19, 66, 439, 494
Ppu21I YACGTR 1 cut(s) 110
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
PspLI CGTACG 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 7, 282
PspPI GGNCC 1 cut(s) 280
PspPPI RGGWCCY 1 cut(s) 280
PstI CTGCAG 1 cut(s) 236
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 1 cut(s) 5
RsaI GTAC 3 cut(s) 89, 108, 486
RsaNI GTAC 3 cut(s) 88, 107, 485
SatI GCNGC 3 cut(s) 232, 235, 460
Sau3AI GATC 2 cut(s) 5, 297
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 4 cut(s) 20, 66, 440, 494
SduI GDGCHC 2 cut(s) 84, 411
SetI ASST 8 cut(s) 112, 135, 174, 212, 262, 369, 450, 509
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 232
SfuI TTCGAA 1 cut(s) 361
SgrAI CRCCGGYG 1 cut(s) 32
SinI GGWCC 1 cut(s) 280
SphI GCATGC 1 cut(s) 342
Sse9I AATT 1 cut(s) 452
SsiI CCGC 6 cut(s) 97, 161, 188, 197, 237, 460
SspMI CTAG 2 cut(s) 77, 504
TaaI ACNGT 6 cut(s) 94, 106, 180, 321, 358, 394
TaiI ACGT 2 cut(s) 112, 450
TaqI TCGA 2 cut(s) 275, 361
TasI AATT 1 cut(s) 452
TauI GCSGC 1 cut(s) 462
TfiI GAWTC 3 cut(s) 17, 121, 272
TscAI CASTG 1 cut(s) 183
TseFI GTSAC 3 cut(s) 28, 110, 443
TseI GCWGC 2 cut(s) 231, 234
Tsp45I GTSAC 3 cut(s) 28, 110, 443
TspDTI ATGAA 3 cut(s) 37, 231, 306
TspGWI ACGGA 1 cut(s) 10
TspRI CASTG 1 cut(s) 183
VpaK11BI GGWCC 1 cut(s) 280
XapI RAATTY 1 cut(s) 452
XceI RCATGY 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 423
XspI CTAG 2 cut(s) 77, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.