pycom15g14510

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002821
Physical Location & Seq
Forward (+)
9910614 .. 9913520
2907 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1038 bp
ATGGCGACGACATCCTCCTCCTCCACTCTCCACCAAACATTTCTATCCATAAACTACCCTCCCCTACATCACAATTCTCAATTCCGCTCTCCTCATCTCTACCGCCCCTCCCGCCATTCCTCCCACCCCATTCGCGCCACCTCCACCGTCACTCTCCAACCGCAGCGGCGGTCGACCGCCGAGGAAAGTAAAAGCTCCGGCGTTGACCTCGAGCTGCTAGCCTGCCCAATATGCTACGAGCCGCTCATCCGGAAAGGCCCGTCGGGTCTCAACCTGCGAGCAATTTACAGGTCGGGGTTCAAGTGCAGGAAATGCGACAAGTCGTATTCGAGCAAAGACATTTACTTGGACCTTACCGTTACTGCTGGGTTGAAGGAGTACGTTGAGGTAAAACCGGCCGGGACCGAGTTGTTCCGGAGCCCTCTTGTTTCCTTCTTGTATGAAAGGAGTTGGCGCCAGAATTTTAACCGCAGGGGATTTCCGGGTCCCGATGAAGAGTTTAAGATGGCTCAGGACTACTTTAAATCCGTGGAAGCCGGTGTTTTGGTGGATGTGAGCTGTGGGAGCGGCTTGTTTTCGAGGAAGTTTGCGAAATCCGGGACATATTCTGGAGTTGTTGCACTTGATTTTTCGGAGAACATGCTTCGGCAGAGTTATGAATTTTTTAAGAGAGATCCTACTCTTTTAGATACTAATCTTGCCCTTGTGAGGGCGGATGTTGCTAGGCTTCCGTTCTCTTCGGGTTCAGTTGATGCAGTCCACGCCGGTGCAGCGTTGCATTGTTGGCCTTCTCCTTCCAATGCTATTGCTGAAATAACTCGCATATTACGAAGTGGTGGTGTCTTTGTCGGAACCACTTTTCTGCGGTATACTCCATCTACTTCCTGGATACCCCGGCAACTCAGAGAGAGGGCTCGTCAGAGCTATAACTATTTAACGGAGGAGGAGATTGCAGACTTGTGTACATCATGCGGTCTCACCAACTACACGAGCAACGTTCAACAATCTTTCATCATGTTTTCTGCTCAGAAACCTTAA

Protein Analysis

346

Amino Acids

38.69

Weight (kDa)

9.21

Isoelectric Point (pI)

56.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 178 - 329 1.1e-10 Methyltransferase domain
Ubie_methyltran PF01209 178 - 286 3.7e-10 ubiE/COQ5 methyltransferase family
Methyltransf_31 PF13847 181 - 306 2.9e-12 Methyltransferase domain
Methyltransf_11 PF08241 183 - 283 4.9e-20 Methyltransferase domain
Methyltransf_25 PF13649 183 - 280 1.7e-16 Methyltransferase domain
Methyltransf_12 PF08242 183 - 282 3.1e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 453
AccBSI CCGCTC 3 cut(s) 87, 244, 567
AccI GTMKAC 2 cut(s) 173, 869
AccII CGCG 1 cut(s) 135
AccIII TCCGGA 2 cut(s) 249, 414
AclI AACGTT 1 cut(s) 996
AclWI GGATC 1 cut(s) 668
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 2 cut(s) 460, 659
AcyI GRCGYC 1 cut(s) 454
AfaI GTAC 2 cut(s) 380, 964
AfiI CCNNNNNNNGG 2 cut(s) 708, 709
AgsI TTSAA 3 cut(s) 301, 373, 1001
AjnI CCWGG 1 cut(s) 884
AleI CACNNNNGTG 1 cut(s) 765
AluBI AGCT 4 cut(s) 195, 214, 558, 924
AluI AGCT 4 cut(s) 195, 214, 558, 924
Alw26I GTCTC 2 cut(s) 272, 980
AlwI GGATC 1 cut(s) 668
Ama87I CYCGRG 1 cut(s) 209
Aor13HI TCCGGA 2 cut(s) 249, 414
AoxI GGCC 3 cut(s) 256, 396, 785
ApeKI GCWGC 3 cut(s) 163, 214, 770
ApoI RAATTY 2 cut(s) 460, 659
AspLEI GCGC 2 cut(s) 137, 456
AspS9I GGNCC 4 cut(s) 257, 349, 402, 485
AsuC2I CCSGG 4 cut(s) 400, 483, 598, 895
AsuHPI GGTGA 1 cut(s) 970
AsuNHI GCTAGC 1 cut(s) 217
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 3 cut(s) 349, 402, 485
BanI GGYRCC 1 cut(s) 453
BanII GRGCYC 2 cut(s) 422, 916
BauI CACGAG 1 cut(s) 988
BbvI GCAGC 3 cut(s) 175, 201, 782
BccI CCATC 2 cut(s) 499, 883
BciT130I CCWGG 1 cut(s) 886
BciVI GTATCC 1 cut(s) 882
BcnI CCSGG 4 cut(s) 400, 483, 598, 895
BcoDI GTCTC 2 cut(s) 272, 980
BfaI CTAG 2 cut(s) 218, 723
BfoI RGCGCY 1 cut(s) 457
BfuAI ACCTGC 1 cut(s) 282
BfuI GTATCC 1 cut(s) 882
BisI GCNGC 6 cut(s) 164, 167, 215, 242, 568, 771
BlsI GCNGC 6 cut(s) 165, 168, 216, 243, 569, 772
Bme1390I CCNGG 5 cut(s) 400, 483, 598, 886, 895
Bme18I GGWCC 3 cut(s) 349, 402, 485
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 4 cut(s) 257, 349, 402, 485
BmiI GGNNCC 6 cut(s) 403, 419, 455, 486, 487, 853
BmrFI CCNGG 5 cut(s) 400, 483, 598, 886, 895
BmsI GCATC 1 cut(s) 742
BmtI GCTAGC 1 cut(s) 221
BpmI CTGGAG 1 cut(s) 630
Bpu10I CCTNAGC 1 cut(s) 510
BpuMI CCSGG 4 cut(s) 400, 483, 598, 895
BsaBI GATNNNNATC 1 cut(s) 693
BsaHI GRCGYC 1 cut(s) 454
BsaI GGTCTC 2 cut(s) 272, 980
BsaJI CCNNGG 3 cut(s) 180, 528, 893
BsaWI WCCGGW 2 cut(s) 249, 414
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 2 cut(s) 708, 709
Bse118I RCCGGY 3 cut(s) 394, 536, 764
Bse8I GATNNNNATC 1 cut(s) 693
BseAI TCCGGA 2 cut(s) 249, 414
BseBI CCWGG 1 cut(s) 886
BseDI CCNNGG 3 cut(s) 180, 528, 893
BseGI GGATG 4 cut(s) 11, 246, 556, 721
BseJI GATNNNNATC 1 cut(s) 693
BseLI CCNNNNNNNGG 2 cut(s) 708, 709
BseMII CTCAG 2 cut(s) 524, 916
BseRI GAGGAG 5 cut(s) 7, 10, 81, 956, 959
BseX3I CGGCCG 1 cut(s) 396
BseXI GCAGC 3 cut(s) 175, 201, 782
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 2 cut(s) 325, 789
Bsh1236I CGCG 1 cut(s) 135
Bsh1285I CGRYCG 3 cut(s) 173, 177, 399
BshFI GGCC 3 cut(s) 258, 398, 787
BshNI GGYRCC 1 cut(s) 453
BsiEI CGRYCG 3 cut(s) 173, 177, 399
BsiHKCI CYCGRG 1 cut(s) 209
BslFI GGGAC 3 cut(s) 415, 471, 613
BslI CCNNNNNNNGG 2 cut(s) 708, 709
BsmAI GTCTC 2 cut(s) 272, 980
BsmFI GGGAC 3 cut(s) 415, 471, 613
BsnI GGCC 3 cut(s) 258, 398, 787
Bso31I GGTCTC 2 cut(s) 272, 980
BsoBI CYCGRG 1 cut(s) 209
Bsp1286I GDGCHC 2 cut(s) 422, 916
Bsp13I TCCGGA 2 cut(s) 249, 414
Bsp1407I TGTACA 1 cut(s) 962
Bsp143I GATC 1 cut(s) 673
BspANI GGCC 3 cut(s) 258, 398, 787
BspCNI CTCAG 2 cut(s) 523, 915
BspEI TCCGGA 2 cut(s) 249, 414
BspFNI CGCG 1 cut(s) 135
BspLI GGNNCC 6 cut(s) 403, 419, 455, 486, 487, 853
BspMI ACCTGC 1 cut(s) 282
BspOI GCTAGC 1 cut(s) 221
BspPI GGATC 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 453
BspTNI GGTCTC 2 cut(s) 272, 980
BsrBI CCGCTC 3 cut(s) 87, 244, 567
BsrFI RCCGGY 3 cut(s) 394, 536, 764
BsrGI TGTACA 1 cut(s) 962
BssAI RCCGGY 3 cut(s) 394, 536, 764
BssECI CCNNGG 3 cut(s) 180, 528, 893
BssMI GATC 1 cut(s) 673
BssNAI GTATAC 1 cut(s) 870
BssNI GRCGYC 1 cut(s) 454
BssSI CACGAG 1 cut(s) 988
Bst1107I GTATAC 1 cut(s) 870
Bst2BI CACGAG 1 cut(s) 988
Bst2UI CCWGG 1 cut(s) 886
Bst4CI ACNGT 2 cut(s) 148, 358
Bst6I CTCTTC 2 cut(s) 489, 742
BstACI GRCGYC 1 cut(s) 454
BstAPI GCANNNNNTGC 1 cut(s) 312
BstAUI TGTACA 1 cut(s) 962
BstC8I GCNNGC 3 cut(s) 219, 223, 279
BstDEI CTNAG 3 cut(s) 510, 902, 1026
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 4 cut(s) 11, 246, 556, 721
BstFNI CGCG 1 cut(s) 135
BstH2I RGCGCY 1 cut(s) 457
BstHHI GCGC 2 cut(s) 137, 456
BstKTI GATC 1 cut(s) 676
BstMAI GTCTC 2 cut(s) 272, 980
BstMBI GATC 1 cut(s) 673
BstMCI CGRYCG 3 cut(s) 173, 177, 399
BstMWI GCNNNNNNNGC 8 cut(s) 111, 231, 312, 564, 719, 761, 770, 784
BstNI CCWGG 1 cut(s) 886
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 5 cut(s) 398, 481, 596, 884, 893
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 3 cut(s) 175, 201, 782
BstX2I RGATCY 1 cut(s) 673
BstYI RGATCY 1 cut(s) 673
BstZ17I GTATAC 1 cut(s) 870
BstZI CGGCCG 1 cut(s) 396
BsuI GTATCC 1 cut(s) 882
BsuRI GGCC 3 cut(s) 258, 398, 787
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 4 cut(s) 11, 246, 556, 721
BveI ACCTGC 1 cut(s) 282
Cac8I GCNNGC 3 cut(s) 219, 223, 279
CfoI GCGC 2 cut(s) 137, 456
Cfr10I RCCGGY 3 cut(s) 394, 536, 764
Cfr13I GGNCC 4 cut(s) 257, 349, 402, 485
Csp6I GTAC 2 cut(s) 379, 963
CviAII CATG 3 cut(s) 640, 969, 1015
CviQI GTAC 2 cut(s) 379, 963
DdeI CTNAG 3 cut(s) 510, 902, 1026
DinI GGCGCC 1 cut(s) 455
DpnI GATC 1 cut(s) 675
DpnII GATC 1 cut(s) 673
DraI TTTAAA 1 cut(s) 523
EaeI YGGCCR 1 cut(s) 396
EagI CGGCCG 1 cut(s) 396
Eam1104I CTCTTC 2 cut(s) 489, 742
EarI CTCTTC 2 cut(s) 489, 742
EciI GGCGGA 1 cut(s) 728
EclXI CGGCCG 1 cut(s) 396
Eco24I GRGCYC 2 cut(s) 422, 916
Eco31I GGTCTC 2 cut(s) 272, 980
Eco47I GGWCC 3 cut(s) 349, 402, 485
Eco52I CGGCCG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 209
EcoO109I RGGNCCY 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 884
EcoT38I GRGCYC 2 cut(s) 422, 916
EgeI GGCGCC 1 cut(s) 455
EheI GGCGCC 1 cut(s) 455
FaeI CATG 3 cut(s) 643, 972, 1018
FaqI GGGAC 3 cut(s) 415, 471, 613
FatI CATG 3 cut(s) 639, 968, 1014
FauI CCCGC 1 cut(s) 119
FblI GTMKAC 2 cut(s) 173, 869
Fnu4HI GCNGC 6 cut(s) 164, 167, 215, 242, 568, 771
FokI GGATG 3 cut(s) 233, 563, 728
FriOI GRGCYC 2 cut(s) 422, 916
Fsp4HI GCNGC 6 cut(s) 164, 167, 215, 242, 568, 771
FspBI CTAG 2 cut(s) 218, 723
GlaI GCGC 2 cut(s) 136, 455
GluI GCNGC 6 cut(s) 164, 167, 215, 242, 568, 771
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 630
HaeII RGCGCY 1 cut(s) 457
HaeIII GGCC 3 cut(s) 258, 398, 787
HhaI GCGC 2 cut(s) 137, 456
Hin1I GRCGYC 1 cut(s) 454
Hin1II CATG 3 cut(s) 643, 972, 1018
Hin6I GCGC 2 cut(s) 135, 454
HinP1I GCGC 2 cut(s) 135, 454
HincII GTYRAC 2 cut(s) 174, 205
HindII GTYRAC 2 cut(s) 174, 205
HphI GGTGA 1 cut(s) 970
Hpy166II GTNNAC 5 cut(s) 174, 205, 760, 870, 963
Hpy188I TCNGA 5 cut(s) 634, 851, 905, 921, 1029
Hpy188III TCNNGA 5 cut(s) 250, 415, 488, 512, 609
Hpy8I GTNNAC 5 cut(s) 174, 205, 760, 870, 963
Hpy99I CGWCG 2 cut(s) 10, 265
HpyAV CCTTC 4 cut(s) 367, 442, 798, 804
HpyCH4III ACNGT 2 cut(s) 148, 358
HpyCH4IV ACGT 2 cut(s) 381, 996
HpyCH4V TGCA 6 cut(s) 306, 620, 755, 770, 778, 953
HpyF10VI GCNNNNNNNGC 8 cut(s) 111, 231, 312, 564, 719, 761, 770, 784
HpyF3I CTNAG 3 cut(s) 510, 902, 1026
HpySE526I ACGT 2 cut(s) 381, 996
Hsp92I GRCGYC 1 cut(s) 454
Hsp92II CATG 3 cut(s) 643, 972, 1018
HspAI GCGC 2 cut(s) 135, 454
KasI GGCGCC 1 cut(s) 453
KflI GGGWCCC 1 cut(s) 485
Kpn2I TCCGGA 2 cut(s) 249, 414
Kzo9I GATC 1 cut(s) 673
LmnI GCTCC 3 cut(s) 200, 417, 564
Lsp1109I GCAGC 3 cut(s) 175, 201, 782
LweI GCATC 1 cut(s) 742
MaeI CTAG 2 cut(s) 218, 723
MaeII ACGT 2 cut(s) 381, 996
MaeIII GTNAC 2 cut(s) 148, 358
MalI GATC 1 cut(s) 675
MbiI CCGCTC 3 cut(s) 87, 244, 567
MboI GATC 1 cut(s) 673
MboII GAAGA 2 cut(s) 506, 729
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 2 cut(s) 422, 916
MluCI AATT 5 cut(s) 73, 80, 282, 460, 659
Mly113I GGCGCC 1 cut(s) 454
MmeI TCCRAC 2 cut(s) 181, 829
MroI TCCGGA 2 cut(s) 249, 414
MseI TTAA 6 cut(s) 465, 501, 522, 666, 935, 1036
MslI CAYNNNNRTG 1 cut(s) 765
MspA1I CMGCKG 1 cut(s) 166
MspR9I CCNGG 5 cut(s) 400, 483, 598, 886, 895
MvaI CCWGG 1 cut(s) 886
MvnI CGCG 1 cut(s) 135
MwoI GCNNNNNNNGC 8 cut(s) 111, 231, 312, 564, 719, 761, 770, 784
NarI GGCGCC 1 cut(s) 454
NciI CCSGG 4 cut(s) 400, 483, 598, 895
NdeII GATC 1 cut(s) 673
NheI GCTAGC 1 cut(s) 217
NlaIII CATG 3 cut(s) 643, 972, 1018
NlaIV GGNNCC 6 cut(s) 403, 419, 455, 486, 487, 853
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 148
NspI RCATGY 1 cut(s) 643
OliI CACNNNNGTG 1 cut(s) 765
PaeR7I CTCGAG 1 cut(s) 209
PcsI WCGNNNNNNNCGW 1 cut(s) 826
PfoI TCCNGGA 2 cut(s) 596, 884
PkrI GCNGC 6 cut(s) 165, 168, 216, 243, 569, 772
PluTI GGCGCC 1 cut(s) 457
PpuMI RGGWCCY 1 cut(s) 485
Psp1406I AACGTT 1 cut(s) 996
Psp5II RGGWCCY 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 884
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 1 cut(s) 884
PspN4I GGNNCC 6 cut(s) 403, 419, 455, 486, 487, 853
PspPI GGNCC 4 cut(s) 257, 349, 402, 485
PspPPI RGGWCCY 1 cut(s) 485
PspXI VCTCGAGB 1 cut(s) 209
PsuI RGATCY 1 cut(s) 673
RsaI GTAC 2 cut(s) 380, 964
RsaNI GTAC 2 cut(s) 379, 963
RseI CAYNNNNRTG 1 cut(s) 765
SalI GTCGAC 1 cut(s) 172
SaqAI TTAA 6 cut(s) 465, 501, 522, 666, 935, 1036
SatI GCNGC 6 cut(s) 164, 167, 215, 242, 568, 771
Sau3AI GATC 1 cut(s) 673
Sau96I GGNCC 4 cut(s) 257, 349, 402, 485
ScrFI CCNGG 5 cut(s) 400, 483, 598, 886, 895
SduI GDGCHC 2 cut(s) 422, 916
SfaNI GCATC 1 cut(s) 742
SfoI GGCGCC 1 cut(s) 455
Sfr274I CTCGAG 1 cut(s) 209
SgrAI CRCCGGYG 1 cut(s) 764
SinI GGWCC 3 cut(s) 349, 402, 485
SlaI CTCGAG 1 cut(s) 209
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 5 cut(s) 73, 80, 282, 460, 659
SspDI GGCGCC 1 cut(s) 453
SspMI CTAG 2 cut(s) 218, 723
StyD4I CCNGG 5 cut(s) 398, 481, 596, 884, 893
TaaI ACNGT 2 cut(s) 148, 358
TaiI ACGT 2 cut(s) 384, 999
TaqI TCGA 4 cut(s) 173, 210, 329, 578
TaqII GACCGA 1 cut(s) 419
TasI AATT 5 cut(s) 73, 80, 282, 460, 659
TatI WGTACW 1 cut(s) 962
TauI GCSGC 3 cut(s) 169, 244, 570
Tru1I TTAA 6 cut(s) 465, 501, 522, 666, 935, 1036
Tru9I TTAA 6 cut(s) 465, 501, 522, 666, 935, 1036
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 3 cut(s) 163, 214, 770
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 4 cut(s) 456, 507, 672, 1000
TspGWI ACGGA 3 cut(s) 517, 720, 953
VpaK11BI GGWCC 3 cut(s) 349, 402, 485
XapI RAATTY 2 cut(s) 460, 659
XceI RCATGY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 882
XhoI CTCGAG 1 cut(s) 209
XmiI GTMKAC 2 cut(s) 173, 869
XspI CTAG 2 cut(s) 218, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.