pycom15g14850

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003259
Physical Location & Seq
Forward (+)
10184756 .. 10186796
2041 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1209 bp
ATGTCTTGGCTCTTCAGCTCTCCCCGCCAACCCGACGACGACCCGCGTGATGTAGAACAACCCAACCAACCGCAAAACGACCTGTCGTTTCTCCGCCAAACCCTCGGCCGCCAAATCCGCGGCGTCGCCACCTTCCTCGCTCCACCTCCATCACCTCCATCACCGCCATCAAACAACGAAGTTCCCTCGTCCAATTCTCTGTCGCAGTCGCAGGCGCTTTTGGGAATCCGGAATGATCTGGTGGAGATCGGCGGGAGCTTCAAGACTGGCCTCTCCCTGCTCTCTACGAACAGCAACAAAGCCGTAACCGAGATCTCCAAGTTCGCATCGAGCTTGCTGCAGCTTCAGAACGAAGCTTTAGAAGAAGGCGGAGACGCTGATGACGACGGCGATGTTGGCGGCGGTCAAGTGCCGGGGATCACGGATGAAGTTCTCGGTTTCGTCGCCGAGATTTCGGTGATGGCGGACTGCTGGATCGACTTTCCTATGACGCTCAATCATGATTTCAGCATGTCGGATGCTCAGAAAGAGCATGCGTCTGCTGTTGAACAATTGGTTCCGGGATTTGCAGATTTAAGAGTCCAGCTTTGCAGTTCCATGAGTGAGGAGCAGTTTTGGATGATCTATTTCTTGTTGCTGCTTCCAAGACTAAATGAACATGATTTTGAGCTTCTATCAACCCCCAAGATTGTTGAAGCAAGAGACGTACTTCTGCACAAACTTCAAAACAAGAGGAACACACAGGAGGATGCCCCAGAGAAGTCAATTCTCGTTTCACCTAAAAGGCACATCCAGGATGGGAAGACACGAGGGGAGGAAGTCTCATCTCAAGAGAAGGAAGCCTTGACTGAGATAGTTAACATGGCAGAAAGGTTAAAGACCGATGACGAAGAGGGCACTGAGCAGTGGTCTGAAGGGGCAAGTATTAGTTCTGGCACTTTAGTGGATGGCCAAAGAAAACATGAACCTGAAGACGATATCTCATTCAGCGATTTAGAGGACGAAGAGAATGACCTTTCTAGCAGACAGTCAGGACTAAGGCAAGGACGGGATGCCAGGGCATGTTCACCTAGTGGATCAAATGACTGGGTTCAGCTGAACCGAAGCTCGGAAACTGAGGGTGGCCAGAAAAAGGCTGGTCGGTCAAAAGGGAAAGATTCAGAAGGTGAGGATTCAAGTGACTGGTTTGCGGTAGATGAATATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

403

Amino Acids

44.28

Weight (kDa)

4.42

Isoelectric Point (pI)

64.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 168 - 220 3.5e-08 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 46, 120
AccIII TCCGGA 1 cut(s) 228
AclWI GGATC 3 cut(s) 425, 482, 1084
AcoI YGGCCR 3 cut(s) 106, 949, 1123
AcuI CTGAAG 3 cut(s) 329, 933, 990
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 1073
AfaI GTAC 1 cut(s) 708
AfiI CCNNNNNNNGG 2 cut(s) 1108, 1132
AgsI TTSAA 5 cut(s) 262, 548, 695, 725, 1176
AjnI CCWGG 2 cut(s) 792, 1055
AleI CACNNNNGTG 1 cut(s) 941
AluBI AGCT 9 cut(s) 18, 258, 333, 343, 356, 586, 670, 1096, 1107
AluI AGCT 9 cut(s) 18, 258, 333, 343, 356, 586, 670, 1096, 1107
Alw26I GTCTC 3 cut(s) 366, 696, 826
AlwI GGATC 3 cut(s) 425, 482, 1084
Aor13HI TCCGGA 1 cut(s) 228
AoxI GGCC 4 cut(s) 106, 268, 949, 1123
ApeKI GCWGC 3 cut(s) 337, 340, 637
AspLEI GCGC 1 cut(s) 217
AsuC2I CCSGG 2 cut(s) 414, 561
AsuHPI GGTGA 6 cut(s) 144, 153, 469, 768, 1059, 1178
BaeGI GKGCMC 1 cut(s) 899
BalI TGGCCA 2 cut(s) 951, 1125
BauI CACGAG 1 cut(s) 807
BbsI GAAGAC 2 cut(s) 809, 978
BbvI GCAGC 3 cut(s) 324, 352, 624
BccI CCATC 6 cut(s) 157, 166, 175, 454, 791, 941
BceAI ACGGC 2 cut(s) 287, 403
BcgI CGANNNNNNTGC 2 cut(s) 319, 353
BciT130I CCWGG 2 cut(s) 794, 1057
BcnI CCSGG 2 cut(s) 414, 561
BcoDI GTCTC 3 cut(s) 366, 696, 826
BfaI CTAG 2 cut(s) 1020, 1071
BfmI CTRYAG 1 cut(s) 338
BfoI RGCGCY 1 cut(s) 218
BglII AGATCT 1 cut(s) 312
BisI GCNGC 6 cut(s) 109, 121, 338, 341, 400, 638
BlsI GCNGC 6 cut(s) 110, 122, 339, 342, 401, 639
Bme1390I CCNGG 4 cut(s) 414, 561, 794, 1057
BmiI GGNNCC 1 cut(s) 558
BmrFI CCNGG 4 cut(s) 414, 561, 794, 1057
BmrI ACTGGG 1 cut(s) 1096
BmsI GCATC 4 cut(s) 335, 508, 739, 1042
BmuI ACTGGG 1 cut(s) 1096
BpiI GAAGAC 2 cut(s) 809, 978
BplI GAGNNNNNCTC 2 cut(s) 806, 838
BpuEI CTTGAG 1 cut(s) 813
BpuMI CCSGG 2 cut(s) 414, 561
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 4 cut(s) 103, 118, 413, 1056
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 2 cut(s) 1108, 1132
Bse1I ACTGG 3 cut(s) 271, 1091, 1187
BseAI TCCGGA 1 cut(s) 228
BseBI CCWGG 2 cut(s) 794, 1057
BseDI CCNNGG 4 cut(s) 103, 118, 413, 1056
BseGI GGATG 8 cut(s) 430, 523, 624, 754, 789, 802, 952, 1057
BseLI CCNNNNNNNGG 2 cut(s) 1108, 1132
BseMII CTCAG 4 cut(s) 536, 840, 891, 1107
BseNI ACTGG 3 cut(s) 271, 1091, 1187
BseRI GAGGAG 1 cut(s) 620
BseSI GKGCMC 1 cut(s) 899
BseX3I CGGCCG 1 cut(s) 106
BseXI GCAGC 3 cut(s) 324, 352, 624
BsgI GTGCAG 1 cut(s) 698
Bsh1236I CGCG 2 cut(s) 46, 120
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 4 cut(s) 108, 270, 951, 1125
BsiEI CGRYCG 1 cut(s) 109
BsiSI CCGG 3 cut(s) 229, 413, 560
BslI CCNNNNNNNGG 2 cut(s) 1108, 1132
BsmAI GTCTC 3 cut(s) 366, 696, 826
BsmBI CGTCTC 2 cut(s) 366, 696
BsnI GGCC 4 cut(s) 108, 270, 951, 1125
Bsp1286I GDGCHC 1 cut(s) 899
Bsp13I TCCGGA 1 cut(s) 228
Bsp143I GATC 7 cut(s) 235, 246, 312, 417, 474, 621, 1076
BspANI GGCC 4 cut(s) 108, 270, 951, 1125
BspCNI CTCAG 4 cut(s) 535, 841, 892, 1108
BspEI TCCGGA 1 cut(s) 228
BspFNI CGCG 2 cut(s) 46, 120
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 1 cut(s) 558
BspMAI CTGCAG 1 cut(s) 342
BspPI GGATC 3 cut(s) 425, 482, 1084
BspQI GCTCTTC 1 cut(s) 17
BsrI ACTGG 3 cut(s) 271, 1091, 1187
BssECI CCNNGG 4 cut(s) 103, 118, 413, 1056
BssMI GATC 7 cut(s) 235, 246, 312, 417, 474, 621, 1076
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 807
Bst2BI CACGAG 1 cut(s) 807
Bst2UI CCWGG 2 cut(s) 794, 1057
Bst4CI ACNGT 1 cut(s) 1029
Bst6I CTCTTC 3 cut(s) 17, 885, 999
BstACI GRCGYC 1 cut(s) 123
BstC8I GCNNGC 3 cut(s) 213, 335, 534
BstDEI CTNAG 5 cut(s) 522, 849, 900, 1037, 1116
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 8 cut(s) 430, 523, 624, 754, 789, 802, 952, 1057
BstFNI CGCG 2 cut(s) 46, 120
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 7 cut(s) 238, 249, 315, 420, 477, 624, 1079
BstMAI GTCTC 3 cut(s) 366, 696, 826
BstMBI GATC 7 cut(s) 235, 246, 312, 417, 474, 621, 1076
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 3 cut(s) 24, 117, 396
BstNI CCWGG 2 cut(s) 794, 1057
BstNSI RCATGY 3 cut(s) 514, 536, 1065
BstSCI CCNGG 4 cut(s) 412, 559, 792, 1055
BstSFI CTRYAG 1 cut(s) 338
BstSLI GKGCMC 1 cut(s) 899
BstUI CGCG 2 cut(s) 46, 120
BstV1I GCAGC 3 cut(s) 324, 352, 624
BstV2I GAAGAC 2 cut(s) 809, 978
BstX2I RGATCY 1 cut(s) 312
BstYI RGATCY 1 cut(s) 312
BstZI CGGCCG 1 cut(s) 106
BsuRI GGCC 4 cut(s) 108, 270, 951, 1125
BtgI CCRYGG 1 cut(s) 118
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 8 cut(s) 430, 523, 624, 754, 789, 802, 952, 1057
BtsI GCAGTG 1 cut(s) 911
BtsIMutI CAGTG 2 cut(s) 897, 911
Cac8I GCNNGC 3 cut(s) 213, 335, 534
CciI TCATGA 1 cut(s) 499
CfoI GCGC 1 cut(s) 217
Cfr42I CCGCGG 1 cut(s) 121
CseI GACGC 4 cut(s) 112, 383, 499, 525
Csp6I GTAC 1 cut(s) 707
CviAII CATG 8 cut(s) 500, 511, 533, 598, 659, 862, 962, 1062
CviQI GTAC 1 cut(s) 707
DdeI CTNAG 5 cut(s) 522, 849, 900, 1037, 1116
DpnI GATC 7 cut(s) 237, 248, 314, 419, 476, 623, 1078
DpnII GATC 7 cut(s) 235, 246, 312, 417, 474, 621, 1076
DraIII CACNNNGTG 1 cut(s) 1073
EaeI YGGCCR 3 cut(s) 106, 949, 1123
EagI CGGCCG 1 cut(s) 106
Eam1104I CTCTTC 3 cut(s) 17, 885, 999
EarI CTCTTC 3 cut(s) 17, 885, 999
EciI GGCGGA 3 cut(s) 83, 384, 479
EclXI CGGCCG 1 cut(s) 106
Eco32I GATATC 1 cut(s) 979
Eco52I CGGCCG 1 cut(s) 106
Eco57I CTGAAG 3 cut(s) 329, 933, 990
EcoRII CCWGG 2 cut(s) 792, 1055
EcoRV GATATC 1 cut(s) 979
Esp3I CGTCTC 2 cut(s) 366, 696
FaeI CATG 8 cut(s) 503, 514, 536, 601, 662, 865, 965, 1065
FalI AAGNNNNNCTT 2 cut(s) 827, 859
FatI CATG 8 cut(s) 499, 510, 532, 597, 658, 861, 961, 1061
FauI CCCGC 3 cut(s) 32, 51, 245
Fnu4HI GCNGC 6 cut(s) 109, 121, 338, 341, 400, 638
FokI GGATG 8 cut(s) 437, 530, 631, 761, 776, 809, 959, 1064
Fsp4HI GCNGC 6 cut(s) 109, 121, 338, 341, 400, 638
FspBI CTAG 2 cut(s) 1020, 1071
GlaI GCGC 1 cut(s) 216
GluI GCNGC 6 cut(s) 109, 121, 338, 341, 400, 638
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 4 cut(s) 108, 270, 951, 1125
HapII CCGG 3 cut(s) 229, 413, 560
HgaI GACGC 4 cut(s) 112, 383, 499, 525
HhaI GCGC 1 cut(s) 217
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 8 cut(s) 503, 514, 536, 601, 662, 865, 965, 1065
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HindIII AAGCTT 1 cut(s) 354
HinfI GANTC 4 cut(s) 225, 579, 1157, 1172
HpaI GTTAAC 1 cut(s) 859
HpaII CCGG 3 cut(s) 229, 413, 560
HphI GGTGA 6 cut(s) 144, 153, 469, 768, 1059, 1178
Hpy166II GTNNAC 2 cut(s) 859, 1067
Hpy188I TCNGA 6 cut(s) 348, 517, 525, 913, 1111, 1162
Hpy188III TCNNGA 5 cut(s) 229, 262, 500, 830, 1032
Hpy8I GTNNAC 2 cut(s) 859, 1067
Hpy99I CGWCG 5 cut(s) 38, 41, 128, 389, 446
HpyAV CCTTC 5 cut(s) 142, 359, 829, 908, 1157
HpyCH4III ACNGT 1 cut(s) 1029
HpyCH4IV ACGT 1 cut(s) 705
HpyCH4V TGCA 4 cut(s) 340, 569, 591, 715
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 117, 396
HpyF3I CTNAG 5 cut(s) 522, 849, 900, 1037, 1116
HpySE526I ACGT 1 cut(s) 705
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 8 cut(s) 503, 514, 536, 601, 662, 865, 965, 1065
HspAI GCGC 1 cut(s) 215
Kpn2I TCCGGA 1 cut(s) 228
KspAI GTTAAC 1 cut(s) 859
KspI CCGCGG 1 cut(s) 121
Kzo9I GATC 7 cut(s) 235, 246, 312, 417, 474, 621, 1076
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 3 cut(s) 145, 255, 607
Lsp1109I GCAGC 3 cut(s) 324, 352, 624
LweI GCATC 4 cut(s) 335, 508, 739, 1042
MaeI CTAG 2 cut(s) 1020, 1071
MaeII ACGT 1 cut(s) 705
MaeIII GTNAC 2 cut(s) 304, 1178
MalI GATC 7 cut(s) 237, 248, 314, 419, 476, 623, 1078
MboI GATC 7 cut(s) 235, 246, 312, 417, 474, 621, 1076
MboII GAAGA 6 cut(s) 4, 374, 814, 902, 983, 1016
MfeI CAATTG 1 cut(s) 551
MflI RGATCY 1 cut(s) 312
MhlI GDGCHC 1 cut(s) 899
MlsI TGGCCA 2 cut(s) 951, 1125
MluCI AATT 3 cut(s) 193, 551, 765
MluNI TGGCCA 2 cut(s) 951, 1125
MlyI GAGTC 1 cut(s) 588
MmeI TCCRAC 1 cut(s) 495
Mox20I TGGCCA 2 cut(s) 951, 1125
MroI TCCGGA 1 cut(s) 228
MscI TGGCCA 2 cut(s) 951, 1125
MseI TTAA 3 cut(s) 575, 858, 875
MslI CAYNNNNRTG 1 cut(s) 941
Msp20I TGGCCA 2 cut(s) 951, 1125
MspA1I CMGCKG 2 cut(s) 120, 1096
MspI CCGG 3 cut(s) 229, 413, 560
MspR9I CCNGG 4 cut(s) 414, 561, 794, 1057
MunI CAATTG 1 cut(s) 551
MvaI CCWGG 2 cut(s) 794, 1057
MvnI CGCG 2 cut(s) 46, 120
MwoI GCNNNNNNNGC 3 cut(s) 24, 117, 396
NciI CCSGG 2 cut(s) 414, 561
NdeII GATC 7 cut(s) 235, 246, 312, 417, 474, 621, 1076
NlaIII CATG 8 cut(s) 503, 514, 536, 601, 662, 865, 965, 1065
NlaIV GGNNCC 1 cut(s) 558
NmeAIII GCCGAG 2 cut(s) 84, 472
NmuCI GTSAC 1 cut(s) 1178
NspI RCATGY 3 cut(s) 514, 536, 1065
OliI CACNNNNGTG 1 cut(s) 941
PaeI GCATGC 1 cut(s) 536
PagI TCATGA 1 cut(s) 499
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PfeI GAWTC 3 cut(s) 225, 1157, 1172
PfoI TCCNGGA 2 cut(s) 559, 792
PkrI GCNGC 6 cut(s) 110, 122, 339, 342, 401, 639
PleI GAGTC 1 cut(s) 587
PpsI GAGTC 1 cut(s) 587
Psp6I CCWGG 2 cut(s) 792, 1055
PspGI CCWGG 2 cut(s) 792, 1055
PspN4I GGNNCC 1 cut(s) 558
PstI CTGCAG 1 cut(s) 342
PsuI RGATCY 1 cut(s) 312
PvuII CAGCTG 1 cut(s) 1096
RsaI GTAC 1 cut(s) 708
RsaNI GTAC 1 cut(s) 707
RseI CAYNNNNRTG 1 cut(s) 941
SacII CCGCGG 1 cut(s) 121
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 3 cut(s) 575, 858, 875
SatI GCNGC 6 cut(s) 109, 121, 338, 341, 400, 638
Sau3AI GATC 7 cut(s) 235, 246, 312, 417, 474, 621, 1076
SchI GAGTC 1 cut(s) 588
ScrFI CCNGG 4 cut(s) 414, 561, 794, 1057
SduI GDGCHC 1 cut(s) 899
SfaNI GCATC 4 cut(s) 335, 508, 739, 1042
SfcI CTRYAG 1 cut(s) 338
Sfr303I CCGCGG 1 cut(s) 121
SgrBI CCGCGG 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 941
SmlI CTYRAG 1 cut(s) 828
SmoI CTYRAG 1 cut(s) 828
SphI GCATGC 1 cut(s) 536
Sse9I AATT 3 cut(s) 193, 551, 765
SspMI CTAG 2 cut(s) 1020, 1071
StyD4I CCNGG 4 cut(s) 412, 559, 792, 1055
TaaI ACNGT 1 cut(s) 1029
TaiI ACGT 1 cut(s) 708
TaqI TCGA 2 cut(s) 329, 477
TaqII GACCGA 2 cut(s) 896, 1131
TasI AATT 3 cut(s) 193, 551, 765
TauI GCSGC 3 cut(s) 111, 123, 402
TfiI GAWTC 3 cut(s) 225, 1157, 1172
Tru1I TTAA 3 cut(s) 575, 858, 875
Tru9I TTAA 3 cut(s) 575, 858, 875
TscAI CASTG 2 cut(s) 904, 911
TseFI GTSAC 1 cut(s) 1178
TseI GCWGC 3 cut(s) 337, 340, 637
Tsp45I GTSAC 1 cut(s) 1178
TspDTI ATGAA 3 cut(s) 441, 669, 978
TspGWI ACGGA 1 cut(s) 437
TspRI CASTG 2 cut(s) 904, 911
XceI RCATGY 3 cut(s) 514, 536, 1065
XcmI CCANNNNNNNNNTGG 1 cut(s) 1133
XspI CTAG 2 cut(s) 1020, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.