pycom15g15380

Primary amine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002010
Physical Location & Seq
Forward (+)
10707811 .. 10708392
582 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
ATGGAAGGTCGGAGATTCCTGCGTTTTTTCTCCCTTTCCCTCTGCATAGGCCTAGTCCTCCTCTTCACTTGGTCCCACCTCCCTTACGCGCCGCCTTACGTCTCCGACCTCCTCGACTGCACAACAAACTCCGCCTGGTGCTCCTCCAAAAACCGCATCCAATCCAAACCCCCTATCCCAACGCGCCGCCTCAAAGACCACAGCTCCGACACCCCCCACCACCCTCTCGACCCCCTCACCATCCAAGAACTCAACGCCGTCCGCACCATCCTCAACTCCCACGCGCTCTTCACCTCCACCTCCGCCTACGCGCTCCACTCCGTCGAGCTCCAGGAGCCCGACAAGTCCCTCGTCCTCGGATGGAAGCACGGCGACCCTCTCTTCCCCAGAAAGGCTTCTATCGTCGCACGTGTCGACAGCAAGTCGCTCGTGCTAACCGTTGACCTGACCACGTCCGAGGTGACCGTTCACGAGACCGGCTCCCACTACGGTTACCCGACGATGACGCTGGAGGAGCTGACCGCTGCCACGTTCAGCCCCCTCAAGAACGCTGATTTCAACCGTACGATCGATCCTGTTTAA

Protein Analysis

194

Amino Acids

21.59

Weight (kDa)

6.75

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_amine_oxidN2 PF02727 74 - 155 1.6e-20 Copper amine oxidase, N2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 414
AccII CGCG 4 cut(s) 89, 184, 284, 311
AciI CCGC 7 cut(s) 92, 132, 154, 187, 262, 303, 522
AclWI GGATC 1 cut(s) 566
AcvI CACGTG 1 cut(s) 410
AfaI GTAC 1 cut(s) 565
AfiI CCNNNNNNNGG 1 cut(s) 391
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 1 cut(s) 559
AhdI GACNNNNNGTC 1 cut(s) 421
AjiI CACGTC 1 cut(s) 453
AjnI CCWGG 2 cut(s) 134, 330
AluBI AGCT 3 cut(s) 204, 328, 517
AluI AGCT 3 cut(s) 204, 328, 517
Alw21I GWGCWC 2 cut(s) 143, 330
Alw26I GTCTC 2 cut(s) 106, 467
AlwI GGATC 1 cut(s) 566
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 1 cut(s) 524
Asp700I GAANNNNTTC 1 cut(s) 394
AspLEI GCGC 4 cut(s) 91, 186, 286, 313
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 3 cut(s) 229, 283, 472
AvaII GGWCC 1 cut(s) 72
BanII GRGCYC 2 cut(s) 330, 339
BauI CACGAG 2 cut(s) 428, 470
BbrPI CACGTG 1 cut(s) 410
Bbv12I GWGCWC 2 cut(s) 143, 330
BbvI GCAGC 1 cut(s) 511
BccI CCATC 3 cut(s) 248, 275, 354
BceAI ACGGC 2 cut(s) 242, 385
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciT130I CCWGG 2 cut(s) 136, 332
BcoDI GTCTC 2 cut(s) 106, 467
BfaI CTAG 1 cut(s) 53
BisI GCNGC 3 cut(s) 92, 187, 525
BlsI GCNGC 3 cut(s) 93, 188, 526
Bme1390I CCNGG 2 cut(s) 136, 332
Bme18I GGWCC 1 cut(s) 72
BmeRI GACNNNNNGTC 1 cut(s) 421
BmgBI CACGTC 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 3 cut(s) 74, 336, 481
BmrFI CCNGG 2 cut(s) 136, 332
BmsI GCATC 1 cut(s) 165
BplI GAGNNNNNCTC 2 cut(s) 464, 496
BpmI CTGGAG 2 cut(s) 314, 530
BpuEI CTTGAG 1 cut(s) 527
Bsa29I ATCGAT 1 cut(s) 570
BsaAI YACGTR 1 cut(s) 410
BsaI GGTCTC 1 cut(s) 467
BsaJI CCNNGG 2 cut(s) 355, 456
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 391
Bse118I RCCGGY 1 cut(s) 476
BseBI CCWGG 2 cut(s) 136, 332
BseCI ATCGAT 1 cut(s) 570
BseDI CCNNGG 2 cut(s) 355, 456
BseGI GGATG 4 cut(s) 156, 240, 267, 365
BseLI CCNNNNNNNGG 1 cut(s) 391
BseRI GAGGAG 4 cut(s) 50, 101, 133, 527
BseXI GCAGC 1 cut(s) 511
BsgI GTGCAG 1 cut(s) 103
Bsh1236I CGCG 4 cut(s) 89, 184, 284, 311
Bsh1285I CGRYCG 1 cut(s) 570
BshFI GGCC 1 cut(s) 51
BshVI ATCGAT 1 cut(s) 570
BsiEI CGRYCG 1 cut(s) 570
BsiHKAI GWGCWC 2 cut(s) 143, 330
BsiSI CCGG 1 cut(s) 477
BsiWI CGTACG 1 cut(s) 563
BslFI GGGAC 2 cut(s) 58, 331
BslI CCNNNNNNNGG 1 cut(s) 391
BsmAI GTCTC 2 cut(s) 106, 467
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 2 cut(s) 58, 331
BsnI GGCC 1 cut(s) 51
Bso31I GGTCTC 1 cut(s) 467
Bsp1286I GDGCHC 3 cut(s) 143, 330, 339
Bsp143I GATC 2 cut(s) 567, 571
BspACI CCGC 7 cut(s) 92, 132, 154, 187, 262, 303, 522
BspANI GGCC 1 cut(s) 51
BspDI ATCGAT 1 cut(s) 570
BspFNI CGCG 4 cut(s) 89, 184, 284, 311
BspLI GGNNCC 3 cut(s) 74, 336, 481
BspPI GGATC 1 cut(s) 566
BspQI GCTCTTC 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 467
BsrFI RCCGGY 1 cut(s) 476
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 2 cut(s) 355, 456
BssMI GATC 2 cut(s) 567, 571
BssSI CACGAG 2 cut(s) 428, 470
Bst2BI CACGAG 2 cut(s) 428, 470
Bst2UI CCWGG 2 cut(s) 136, 332
Bst4CI ACNGT 4 cut(s) 439, 466, 491, 563
Bst6I CTCTTC 3 cut(s) 68, 293, 386
BstBAI YACGTR 1 cut(s) 410
BstEII GGTNACC 2 cut(s) 460, 491
BstF5I GGATG 4 cut(s) 156, 240, 267, 365
BstFNI CGCG 4 cut(s) 89, 184, 284, 311
BstHHI GCGC 4 cut(s) 91, 186, 286, 313
BstKTI GATC 2 cut(s) 570, 574
BstMAI GTCTC 2 cut(s) 106, 467
BstMBI GATC 2 cut(s) 567, 571
BstMCI CGRYCG 1 cut(s) 570
BstMWI GCNNNNNNNGC 2 cut(s) 334, 514
BstNI CCWGG 2 cut(s) 136, 332
BstPI GGTNACC 2 cut(s) 460, 491
BstSCI CCNGG 2 cut(s) 134, 330
BstUI CGCG 4 cut(s) 89, 184, 284, 311
BstV1I GCAGC 1 cut(s) 511
Bsu15I ATCGAT 1 cut(s) 570
BsuRI GGCC 1 cut(s) 51
BsuTUI ATCGAT 1 cut(s) 570
BtrI CACGTC 1 cut(s) 453
BtsCI GGATG 4 cut(s) 156, 240, 267, 365
CfoI GCGC 4 cut(s) 91, 186, 286, 313
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 570
CseI GACGC 1 cut(s) 514
Csp6I GTAC 1 cut(s) 564
CviJI RGCY 8 cut(s) 51, 204, 328, 337, 395, 480, 517, 537
CviKI_1 RGCY 8 cut(s) 51, 204, 328, 337, 395, 480, 517, 537
CviQI GTAC 1 cut(s) 564
DpnI GATC 2 cut(s) 569, 573
DpnII GATC 2 cut(s) 567, 571
DriI GACNNNNNGTC 1 cut(s) 421
Eam1104I CTCTTC 3 cut(s) 68, 293, 386
Eam1105I GACNNNNNGTC 1 cut(s) 421
EarI CTCTTC 3 cut(s) 68, 293, 386
EciI GGCGGA 2 cut(s) 121, 292
Ecl136II GAGCTC 1 cut(s) 328
Eco147I AGGCCT 1 cut(s) 51
Eco24I GRGCYC 2 cut(s) 330, 339
Eco31I GGTCTC 1 cut(s) 467
Eco47I GGWCC 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 328
Eco72I CACGTG 1 cut(s) 410
Eco91I GGTNACC 2 cut(s) 460, 491
EcoICRI GAGCTC 1 cut(s) 328
EcoO65I GGTNACC 2 cut(s) 460, 491
EcoRII CCWGG 2 cut(s) 134, 330
EcoT38I GRGCYC 2 cut(s) 330, 339
Esp3I CGTCTC 1 cut(s) 106
FaiI YATR 1 cut(s) 47
FaqI GGGAC 2 cut(s) 58, 331
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 3 cut(s) 92, 187, 525
FokI GGATG 4 cut(s) 143, 227, 254, 372
FriOI GRGCYC 2 cut(s) 330, 339
Fsp4HI GCNGC 3 cut(s) 92, 187, 525
FspBI CTAG 1 cut(s) 53
GlaI GCGC 4 cut(s) 90, 185, 285, 312
GluI GCNGC 3 cut(s) 92, 187, 525
GsuI CTGGAG 2 cut(s) 314, 530
HaeIII GGCC 1 cut(s) 51
HapII CCGG 1 cut(s) 477
HgaI GACGC 1 cut(s) 514
HhaI GCGC 4 cut(s) 91, 186, 286, 313
Hin6I GCGC 4 cut(s) 89, 184, 284, 311
HinP1I GCGC 4 cut(s) 89, 184, 284, 311
HincII GTYRAC 2 cut(s) 415, 442
HindII GTYRAC 2 cut(s) 415, 442
HinfI GANTC 1 cut(s) 15
HpaII CCGG 1 cut(s) 477
HphI GGTGA 3 cut(s) 229, 283, 472
Hpy166II GTNNAC 3 cut(s) 415, 442, 469
Hpy188I TCNGA 5 cut(s) 12, 106, 208, 359, 457
Hpy188III TCNNGA 3 cut(s) 227, 470, 544
Hpy8I GTNNAC 3 cut(s) 415, 442, 469
Hpy99I CGWCG 3 cut(s) 326, 407, 502
HpyCH4III ACNGT 4 cut(s) 439, 466, 491, 563
HpyCH4IV ACGT 4 cut(s) 99, 409, 452, 530
HpyCH4V TGCA 2 cut(s) 45, 120
HpyF10VI GCNNNNNNNGC 2 cut(s) 334, 514
HpySE526I ACGT 4 cut(s) 99, 409, 452, 530
HspAI GCGC 4 cut(s) 89, 184, 284, 311
Kzo9I GATC 2 cut(s) 567, 571
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 7 cut(s) 146, 209, 318, 333, 334, 485, 514
LpnPI CCDG 9 cut(s) 32, 121, 148, 317, 344, 400, 458, 490, 494
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 1 cut(s) 165
MaeI CTAG 1 cut(s) 53
MaeII ACGT 4 cut(s) 99, 409, 452, 530
MaeIII GTNAC 2 cut(s) 460, 491
MalI GATC 2 cut(s) 569, 573
MboI GATC 2 cut(s) 567, 571
MboII GAAGA 3 cut(s) 55, 280, 373
MhlI GDGCHC 3 cut(s) 143, 330, 339
MmeI TCCRAC 2 cut(s) 129, 231
MroXI GAANNNNTTC 1 cut(s) 394
MseI TTAA 1 cut(s) 580
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 1 cut(s) 477
MspR9I CCNGG 2 cut(s) 136, 332
MvaI CCWGG 2 cut(s) 136, 332
MvnI CGCG 4 cut(s) 89, 184, 284, 311
MwoI GCNNNNNNNGC 2 cut(s) 334, 514
NdeII GATC 2 cut(s) 567, 571
NlaIV GGNNCC 3 cut(s) 74, 336, 481
NmuCI GTSAC 1 cut(s) 460
PceI AGGCCT 1 cut(s) 51
PciSI GCTCTTC 1 cut(s) 293
PcsI WCGNNNNNNNCGW 2 cut(s) 411, 435
PdmI GAANNNNTTC 1 cut(s) 394
PfeI GAWTC 1 cut(s) 15
Pfl23II CGTACG 1 cut(s) 563
PflFI GACNNNGTC 1 cut(s) 451
PfoI TCCNGGA 1 cut(s) 330
PkrI GCNGC 3 cut(s) 93, 188, 526
Ple19I CGATCG 1 cut(s) 570
PmaCI CACGTG 1 cut(s) 410
PmlI CACGTG 1 cut(s) 410
Ppu21I YACGTR 1 cut(s) 410
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 134, 330
PspCI CACGTG 1 cut(s) 410
PspEI GGTNACC 2 cut(s) 460, 491
PspGI CCWGG 2 cut(s) 134, 330
PspLI CGTACG 1 cut(s) 563
PspN4I GGNNCC 3 cut(s) 74, 336, 481
PspPI GGNCC 1 cut(s) 72
PsyI GACNNNGTC 1 cut(s) 451
PvuI CGATCG 1 cut(s) 570
RsaI GTAC 1 cut(s) 565
RsaNI GTAC 1 cut(s) 564
SacI GAGCTC 1 cut(s) 330
SalI GTCGAC 1 cut(s) 413
SapI GCTCTTC 1 cut(s) 293
SaqAI TTAA 1 cut(s) 580
SatI GCNGC 3 cut(s) 92, 187, 525
Sau3AI GATC 2 cut(s) 567, 571
Sau96I GGNCC 1 cut(s) 72
ScrFI CCNGG 2 cut(s) 136, 332
SduI GDGCHC 3 cut(s) 143, 330, 339
SfaNI GCATC 1 cut(s) 165
SinI GGWCC 1 cut(s) 72
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
SseBI AGGCCT 1 cut(s) 51
SsiI CCGC 7 cut(s) 92, 132, 154, 187, 262, 303, 522
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 1 cut(s) 330
StuI AGGCCT 1 cut(s) 51
StyD4I CCNGG 2 cut(s) 134, 330
TaaI ACNGT 4 cut(s) 439, 466, 491, 563
TaiI ACGT 4 cut(s) 102, 412, 455, 533
TaqI TCGA 5 cut(s) 114, 228, 324, 414, 570
TauI GCSGC 2 cut(s) 94, 189
TfiI GAWTC 1 cut(s) 15
Tru1I TTAA 1 cut(s) 580
Tru9I TTAA 1 cut(s) 580
TseFI GTSAC 1 cut(s) 460
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 1 cut(s) 460
TspGWI ACGGA 1 cut(s) 310
Tth111I GACNNNGTC 1 cut(s) 451
VpaK11BI GGWCC 1 cut(s) 72
XmiI GTMKAC 1 cut(s) 414
XmnI GAANNNNTTC 1 cut(s) 394
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.