Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002010

Physical Location & Seq

Genomic Coordinates Reverse (-)

49417405 .. 49422097

Gene Structure 4693 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00255140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1050 bp

ATGTTGTCCTCGAACGACTCCGGGCTTGAACCCCGTACTTTATCTGTGTATTTTCTCATGGAAACCCGAAACTTCTTCAGCCTTTTCTTTCTTTCGCTCTGCGTCGGATTTGTCCTCCTCTTCACTTGGGGTCACCTCCCTTACGCGCCTCCATACACGACTGACCTTCTCGACTGCACTACCAACTCAGCATGGTGCGCCTCCAAAAACCGCTGTCAGTCCAAACCGTCCAGCAACCTCATCAAAAACAAAAACCACCGCAGCCACAACCACGCCTCCGACACTCCCCACCACCCTCTCGACCCCCTCACAGTCCAAGAACTCAACAAAATCCGAACCATCCTCTCCTCCCACGCGCTCTTCAAGTCCTCCTCAGCCTACGCGCTCCACTCCATCGAGCTCGAAGAGCCCGACAAGCCTCTCGTCCTCCGATGGAAAAAGGGCGACCCGCTGCTACCCAGAAAGGCCTCCGTCATCGCACGTGCCTCTGGGAAGGCACACGTGCTGACCGTTGACCTGGCCACGAGCGAGGTGTCCGTACAGCAGACCGGAACCCACTTCGGCTATCCGATGCTGACCATGGAGGAAATAACCGCCGCGATCTGGGTCCCACTTTCGAACGCCAAGTTCAACCGCACGATCATCCAGCGCGGCGTTGATCTGGCGGACATTGCGTGTTTGCCGATCTCGTCGGGGTGGTTCGATGAGTACGAGGAGAACAGGAGGAGGTTGATTAAGGTACAGTGCTTCTCCACGAAGGACACTGTGAACTTTTACATGAGGCCAATCGAAGGATTGACCGTTCTCGTTGACTTGGACGCCGAACAAGTGGTGGAGATTTCCGACAAGGGTGGGAACATTCCGATACCGAAGGCCGCCAACACAGACTATAGATACTCCGCCAGCCAGGAGGTCAGTCGAGTGATTAAGCTAGTGAATCCCATTTCCATTGAGCAACCAAAAGGTCCAAGTTTTACCGTGGAGGACGGTCACTTAGTAAAATGGGCAAATTGGGAATTTCATGTAAAACCCGAAGCCGAGCCGGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0001505 GO:0006807 GO:0006809 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009987 GO:0010033 GO:0017144 GO:0023052 GO:0032870 GO:0033993 GO:0034641 GO:0042133 GO:0042136 GO:0042221 GO:0044237 GO:0044249 GO:0044271 GO:0046209 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0065008 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0072593 GO:0097305 GO:0097306 GO:1901700 GO:1901701 GO:1903409 GO:2001057

KEGG Pathways

Metabolic & Signaling

ko00260 ko00350 ko00360 ko00410 ko00950 ko00960 ko01100 ko01110 map00260 map00350 map00360 map00410 map00950 map00960 map01100 map01110

Pfam Domains

Protein Families

Cu_amine_oxid Cu_amine_oxidN2 Cu_amine_oxidN3

Protein Analysis

349

Amino Acids

39.18

Weight (kDa)

6.64

Isoelectric Point (pI)

39.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cu_amine_oxidN2	PF02727	98 - 179	7.4e-24	Copper amine oxidase, N2 domain
Cu_amine_oxidN3	PF02728	190 - 290	2.5e-24	Copper amine oxidase, N3 domain
Cu_amine_oxid	PF01179	316 - 344	8.8e-06	Copper amine oxidase, enzyme domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002010)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G12270 AT4G12280 AT4G12290 AT4G12290
fragaria_vesca	FvH4_5g00610 FvH4_5g00610 FvH4_5g00610 FvH4_5g00610 FvH4_5g00610
malus_domestica	MD02G1257400.v1.1 MD06G1075500.v1.1 MD16G1192400.v1.1
prunus_persica	Prupe.5G078700_v2.0.a1 Prupe.5G078900_v2.0.a1
pyrus_communis	pycom09g07970 pycom13g04890 pycom15g15380 pycom15g15390
rosa_chinensis	RchiOBHm_Chr5g0060271 RchiOBHm_Chr7g0201651 RchiOBHm_Chr7g0201661
rosa_laevigata	RLG00000003658
rosa_multiflora	Rmu_sc0001512.1_g000015 Rmu_sc0004811.1_g000006 Rmu_sc0004811.1_g000008 Rmu_sc0004811.1_g000009 Rmu_sc0004811.1_g000010 Rmu_sc0004811.1_g000011
rosa_roxburghii	Rroxscaffold_3G00255130 Rroxscaffold_3G00255140 Rroxscaffold_3G00255150 Rroxscaffold_6G00396720
rosa_rugosa	Rorug07G0065300 Rorug07G0065300
rosa_samantha	Rh7AG192300 Rh7AG192500 Rh7BG194400 Rh7BG194500 Rh7BG194600 Rh7CG203500 Rh7CG203600 Rh7CG203700 Rh7DG197700
rosa_wichuraiana	Rw7G016860 Rw7G016870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	5 cut(s)	146, 356, 383, 599, 651
AcoI	YGGCCR	1 cut(s)	519
AcsI	RAATTY	1 cut(s)	1016
AcuI	CTGAAG	1 cut(s)	61
AcvI	CACGTG	2 cut(s)	482, 502
AcyI	GRCGYC	1 cut(s)	819
AfaI	GTAC	4 cut(s)	37, 540, 710, 741
AfiI	CCNNNNNNNGG	2 cut(s)	603, 791
AflIII	ACRYGT	1 cut(s)	499
AgsI	TTSAA	3 cut(s)	29, 364, 631
AjnI	CCWGG	2 cut(s)	516, 906
AloI	GAACNNNNNNTCC	2 cut(s)	786, 818
AluBI	AGCT	2 cut(s)	400, 931
AluI	AGCT	2 cut(s)	400, 931
Alw21I	GWGCWC	1 cut(s)	402
AoxI	GGCC	4 cut(s)	465, 519, 782, 873
ApeKI	GCWGC	2 cut(s)	261, 451
ApoI	RAATTY	1 cut(s)	1016
AspLEI	GCGC	5 cut(s)	148, 200, 358, 385, 651
AspS9I	GGNCC	2 cut(s)	607, 965
AsuC2I	CCSGG	1 cut(s)	22
AsuHPI	GGTGA	1 cut(s)	125
AsuII	TTCGAA	1 cut(s)	617
AvaII	GGWCC	2 cut(s)	607, 965
BalI	TGGCCA	1 cut(s)	521
BanII	GRGCYC	2 cut(s)	402, 411
BauI	CACGAG	1 cut(s)	523
BbrPI	CACGTG	2 cut(s)	482, 502
Bbv12I	GWGCWC	1 cut(s)	402
BbvCI	CCTCAGC	1 cut(s)	373
BbvI	GCAGC	2 cut(s)	273, 438
BccI	CCATC	3 cut(s)	347, 401, 426
BciT130I	CCWGG	2 cut(s)	518, 908
BcnI	CCSGG	1 cut(s)	22
BfaI	CTAG	1 cut(s)	932
BfmI	CTRYAG	1 cut(s)	889
BglI	GCCNNNNNGGC	1 cut(s)	1043
BisI	GCNGC	5 cut(s)	262, 452, 597, 652, 876
BlsI	GCNGC	5 cut(s)	263, 453, 598, 653, 877
Bme1390I	CCNGG	3 cut(s)	22, 518, 908
Bme18I	GGWCC	2 cut(s)	607, 965
BmgT120I	GGNCC	2 cut(s)	607, 965
BmiI	GGNNCC	3 cut(s)	553, 608, 609
BmrFI	CCNGG	3 cut(s)	22, 518, 908
BmsI	GCATC	1 cut(s)	561
Bpu10I	CCTNAGC	1 cut(s)	373
Bpu14I	TTCGAA	1 cut(s)	617
BpuMI	CCSGG	1 cut(s)	22
BsaAI	YACGTR	2 cut(s)	482, 502
BsaHI	GRCGYC	1 cut(s)	819
BsaJI	CCNNGG	2 cut(s)	579, 978
BsaWI	WCCGGW	1 cut(s)	548
BsaXI	ACNNNNNCTCC	2 cut(s)	260, 290
Bsc4I	CCNNNNNNNGG	2 cut(s)	603, 791
Bse118I	RCCGGY	1 cut(s)	1042
Bse3DI	GCAATG	1 cut(s)	669
BseBI	CCWGG	2 cut(s)	518, 908
BseDI	CCNNGG	2 cut(s)	579, 978
BseGI	GGATG	2 cut(s)	339, 642
BseLI	CCNNNNNNNGG	2 cut(s)	603, 791
BseMI	GCAATG	1 cut(s)	669
BseMII	CTCAG	2 cut(s)	201, 387
BseRI	GAGGAG	5 cut(s)	107, 337, 361, 728, 739
BseXI	GCAGC	2 cut(s)	273, 438
BsgI	GTGCAG	1 cut(s)	160
Bsh1236I	CGCG	5 cut(s)	146, 356, 383, 599, 651
BshFI	GGCC	4 cut(s)	467, 521, 784, 875
BsiHKAI	GWGCWC	1 cut(s)	402
BsiSI	CCGG	3 cut(s)	21, 549, 1043
BslFI	GGGAC	1 cut(s)	593
BslI	CCNNNNNNNGG	2 cut(s)	603, 791
BsmFI	GGGAC	1 cut(s)	593
BsnI	GGCC	4 cut(s)	467, 521, 784, 875
Bsp119I	TTCGAA	1 cut(s)	617
Bsp1286I	GDGCHC	2 cut(s)	402, 411
Bsp143I	GATC	4 cut(s)	600, 639, 658, 684
Bsp19I	CCATGG	1 cut(s)	579
BspANI	GGCC	4 cut(s)	467, 521, 784, 875
BspCNI	CTCAG	2 cut(s)	200, 386
BspFNI	CGCG	5 cut(s)	146, 356, 383, 599, 651
BspLI	GGNNCC	3 cut(s)	553, 608, 609
BspQI	GCTCTTC	2 cut(s)	365, 399
BspT104I	TTCGAA	1 cut(s)	617
BsrDI	GCAATG	1 cut(s)	669
BsrFI	RCCGGY	1 cut(s)	1042
BssAI	RCCGGY	1 cut(s)	1042
BssECI	CCNNGG	2 cut(s)	579, 978
BssMI	GATC	4 cut(s)	600, 639, 658, 684
BssNI	GRCGYC	1 cut(s)	819
BssSI	CACGAG	1 cut(s)	523
BssT1I	CCWWGG	1 cut(s)	579
Bst2BI	CACGAG	1 cut(s)	523
Bst2UI	CCWGG	2 cut(s)	518, 908
Bst4CI	ACNGT	8 cut(s)	228, 313, 511, 744, 766, 802, 979, 989
Bst6I	CTCTTC	3 cut(s)	125, 365, 399
BstACI	GRCGYC	1 cut(s)	819
BstBAI	YACGTR	2 cut(s)	482, 502
BstBI	TTCGAA	1 cut(s)	617
BstC8I	GCNNGC	2 cut(s)	904, 1044
BstDEI	CTNAG	3 cut(s)	187, 373, 994
BstDSI	CCRYGG	2 cut(s)	579, 978
BstEII	GGTNACC	1 cut(s)	131
BstF5I	GGATG	2 cut(s)	339, 642
BstFNI	CGCG	5 cut(s)	146, 356, 383, 599, 651
BstHHI	GCGC	5 cut(s)	148, 200, 358, 385, 651
BstKTI	GATC	4 cut(s)	603, 642, 661, 687
BstMBI	GATC	4 cut(s)	600, 639, 658, 684
BstMWI	GCNNNNNNNGC	5 cut(s)	197, 406, 415, 671, 1043
BstNI	CCWGG	2 cut(s)	518, 908
BstPI	GGTNACC	1 cut(s)	131
BstSCI	CCNGG	3 cut(s)	20, 516, 906
BstSFI	CTRYAG	1 cut(s)	889
BstUI	CGCG	5 cut(s)	146, 356, 383, 599, 651
BstV1I	GCAGC	2 cut(s)	273, 438
BsuRI	GGCC	4 cut(s)	467, 521, 784, 875
BtgI	CCRYGG	2 cut(s)	579, 978
BtgZI	GCGATG	1 cut(s)	460
BtsCI	GGATG	2 cut(s)	339, 642
BtsIMutI	CAGTG	2 cut(s)	749, 762
Cac8I	GCNNGC	2 cut(s)	904, 1044
CfoI	GCGC	5 cut(s)	148, 200, 358, 385, 651
Cfr10I	RCCGGY	1 cut(s)	1042
Cfr13I	GGNCC	2 cut(s)	607, 965
CseI	GACGC	2 cut(s)	91, 827
Csp6I	GTAC	4 cut(s)	36, 539, 709, 740
CviAII	CATG	5 cut(s)	58, 192, 580, 778, 1022
CviQI	GTAC	4 cut(s)	36, 539, 709, 740
DdeI	CTNAG	3 cut(s)	187, 373, 994
DpnI	GATC	4 cut(s)	602, 641, 660, 686
DpnII	GATC	4 cut(s)	600, 639, 658, 684
EaeI	YGGCCR	1 cut(s)	519
Eam1104I	CTCTTC	3 cut(s)	125, 365, 399
EarI	CTCTTC	3 cut(s)	125, 365, 399
EciI	GGCGGA	2 cut(s)	680, 889
Ecl136II	GAGCTC	1 cut(s)	400
Eco130I	CCWWGG	1 cut(s)	579
Eco147I	AGGCCT	1 cut(s)	467
Eco24I	GRGCYC	2 cut(s)	402, 411
Eco47I	GGWCC	2 cut(s)	607, 965
Eco53kI	GAGCTC	1 cut(s)	400
Eco57I	CTGAAG	1 cut(s)	61
Eco72I	CACGTG	2 cut(s)	482, 502
Eco91I	GGTNACC	1 cut(s)	131
EcoICRI	GAGCTC	1 cut(s)	400
EcoO109I	RGGNCCY	1 cut(s)	607
EcoO65I	GGTNACC	1 cut(s)	131
EcoRII	CCWGG	2 cut(s)	516, 906
EcoT14I	CCWWGG	1 cut(s)	579
EcoT38I	GRGCYC	2 cut(s)	402, 411
ErhI	CCWWGG	1 cut(s)	579
FaeI	CATG	5 cut(s)	61, 195, 583, 781, 1025
FaiI	YATR	7 cut(s)	59, 154, 193, 581, 779, 891, 1023
FaqI	GGGAC	1 cut(s)	593
FatI	CATG	5 cut(s)	57, 191, 579, 777, 1021
FauI	CCCGC	1 cut(s)	456
Fnu4HI	GCNGC	5 cut(s)	262, 452, 597, 652, 876
FokI	GGATG	2 cut(s)	326, 629
FriOI	GRGCYC	2 cut(s)	402, 411
Fsp4HI	GCNGC	5 cut(s)	262, 452, 597, 652, 876
FspBI	CTAG	1 cut(s)	932
GlaI	GCGC	5 cut(s)	147, 199, 357, 384, 650
GluI	GCNGC	5 cut(s)	262, 452, 597, 652, 876
HaeIII	GGCC	4 cut(s)	467, 521, 784, 875
HapII	CCGG	3 cut(s)	21, 549, 1043
HgaI	GACGC	2 cut(s)	91, 827
HhaI	GCGC	5 cut(s)	148, 200, 358, 385, 651
Hin1I	GRCGYC	1 cut(s)	819
Hin1II	CATG	5 cut(s)	61, 195, 583, 781, 1025
Hin6I	GCGC	5 cut(s)	146, 198, 356, 383, 649
HinP1I	GCGC	5 cut(s)	146, 198, 356, 383, 649
HincII	GTYRAC	2 cut(s)	514, 811
HindII	GTYRAC	2 cut(s)	514, 811
HinfI	GANTC	2 cut(s)	17, 937
HpaII	CCGG	3 cut(s)	21, 549, 1043
HphI	GGTGA	1 cut(s)	125
Hpy166II	GTNNAC	3 cut(s)	514, 769, 811
Hpy188I	TCNGA	7 cut(s)	107, 280, 335, 431, 570, 844, 864
Hpy188III	TCNNGA	2 cut(s)	170, 299
Hpy8I	GTNNAC	3 cut(s)	514, 769, 811
Hpy99I	CGWCG	2 cut(s)	107, 694
HpyAV	CCTTC	5 cut(s)	176, 487, 751, 785, 865
HpyCH4III	ACNGT	8 cut(s)	228, 313, 511, 744, 766, 802, 979, 989
HpyCH4IV	ACGT	2 cut(s)	481, 501
HpyCH4V	TGCA	1 cut(s)	177
HpyF10VI	GCNNNNNNNGC	5 cut(s)	197, 406, 415, 671, 1043
HpyF3I	CTNAG	3 cut(s)	187, 373, 994
HpySE526I	ACGT	2 cut(s)	481, 501
Hsp92I	GRCGYC	1 cut(s)	819
Hsp92II	CATG	5 cut(s)	61, 195, 583, 781, 1025
HspAI	GCGC	5 cut(s)	146, 198, 356, 383, 649
KflI	GGGWCCC	1 cut(s)	607
KroI	GCCGGC	1 cut(s)	1042
KroNI	GCCGGC	1 cut(s)	1044
Kzo9I	GATC	4 cut(s)	600, 639, 658, 684
LguI	GCTCTTC	2 cut(s)	365, 399
LmnI	GCTCC	1 cut(s)	390
Lsp1109I	GCAGC	2 cut(s)	273, 438
LweI	GCATC	1 cut(s)	561
MaeI	CTAG	1 cut(s)	932
MaeII	ACGT	2 cut(s)	481, 501
MaeIII	GTNAC	2 cut(s)	131, 989
MalI	GATC	4 cut(s)	602, 641, 660, 686
MboI	GATC	4 cut(s)	600, 639, 658, 684
MboII	GAAGA	4 cut(s)	67, 112, 352, 416
MhlI	GDGCHC	2 cut(s)	402, 411
MlsI	TGGCCA	1 cut(s)	521
MluCI	AATT	2 cut(s)	1009, 1016
MluNI	TGGCCA	1 cut(s)	521
MlyI	GAGTC	1 cut(s)	11
MmeI	TCCRAC	3 cut(s)	85, 303, 867
Mox20I	TGGCCA	1 cut(s)	521
MroNI	GCCGGC	1 cut(s)	1042
MscI	TGGCCA	1 cut(s)	521
MseI	TTAA	2 cut(s)	735, 927
Msp20I	TGGCCA	1 cut(s)	521
MspA1I	CMGCKG	2 cut(s)	213, 451
MspI	CCGG	3 cut(s)	21, 549, 1043
MspR9I	CCNGG	3 cut(s)	22, 518, 908
MvaI	CCWGG	2 cut(s)	518, 908
MvnI	CGCG	5 cut(s)	146, 356, 383, 599, 651
MwoI	GCNNNNNNNGC	5 cut(s)	197, 406, 415, 671, 1043
NaeI	GCCGGC	1 cut(s)	1044
NciI	CCSGG	1 cut(s)	22
NcoI	CCATGG	1 cut(s)	579
NdeII	GATC	4 cut(s)	600, 639, 658, 684
NgoMIV	GCCGGC	1 cut(s)	1042
NlaIII	CATG	5 cut(s)	61, 195, 583, 781, 1025
NlaIV	GGNNCC	3 cut(s)	553, 608, 609
NmuCI	GTSAC	2 cut(s)	131, 989
NspV	TTCGAA	1 cut(s)	617
PceI	AGGCCT	1 cut(s)	467
PciSI	GCTCTTC	2 cut(s)	365, 399
PcsI	WCGNNNNNNNCGW	3 cut(s)	408, 507, 708
PdiI	GCCGGC	1 cut(s)	1044
PfeI	GAWTC	1 cut(s)	937
PkrI	GCNGC	5 cut(s)	263, 453, 598, 653, 877
PleI	GAGTC	1 cut(s)	11
PmaCI	CACGTG	2 cut(s)	482, 502
PmlI	CACGTG	2 cut(s)	482, 502
PpsI	GAGTC	1 cut(s)	11
Ppu21I	YACGTR	2 cut(s)	482, 502
PpuMI	RGGWCCY	1 cut(s)	607
Psp124BI	GAGCTC	1 cut(s)	402
Psp5II	RGGWCCY	1 cut(s)	607
Psp6I	CCWGG	2 cut(s)	516, 906
PspCI	CACGTG	2 cut(s)	482, 502
PspEI	GGTNACC	1 cut(s)	131
PspGI	CCWGG	2 cut(s)	516, 906
PspN4I	GGNNCC	3 cut(s)	553, 608, 609
PspPI	GGNCC	2 cut(s)	607, 965
PspPPI	RGGWCCY	1 cut(s)	607
RsaI	GTAC	4 cut(s)	37, 540, 710, 741
RsaNI	GTAC	4 cut(s)	36, 539, 709, 740
SacI	GAGCTC	1 cut(s)	402
SapI	GCTCTTC	2 cut(s)	365, 399
SaqAI	TTAA	2 cut(s)	735, 927
SatI	GCNGC	5 cut(s)	262, 452, 597, 652, 876
Sau3AI	GATC	4 cut(s)	600, 639, 658, 684
Sau96I	GGNCC	2 cut(s)	607, 965
SchI	GAGTC	1 cut(s)	11
ScrFI	CCNGG	3 cut(s)	22, 518, 908
SduI	GDGCHC	2 cut(s)	402, 411
SfaNI	GCATC	1 cut(s)	561
SfcI	CTRYAG	1 cut(s)	889
SfuI	TTCGAA	1 cut(s)	617
SinI	GGWCC	2 cut(s)	607, 965
Sse9I	AATT	2 cut(s)	1009, 1016
SseBI	AGGCCT	1 cut(s)	467
SspMI	CTAG	1 cut(s)	932
SstI	GAGCTC	1 cut(s)	402
StuI	AGGCCT	1 cut(s)	467
StyD4I	CCNGG	3 cut(s)	20, 516, 906
StyI	CCWWGG	1 cut(s)	579
TaaI	ACNGT	8 cut(s)	228, 313, 511, 744, 766, 802, 979, 989
TaiI	ACGT	2 cut(s)	484, 504
TaqI	TCGA	9 cut(s)	11, 171, 300, 396, 402, 617, 702, 789, 919
TasI	AATT	2 cut(s)	1009, 1016
TauI	GCSGC	3 cut(s)	599, 654, 878
TfiI	GAWTC	1 cut(s)	937
Tru1I	TTAA	2 cut(s)	735, 927
Tru9I	TTAA	2 cut(s)	735, 927
TscAI	CASTG	2 cut(s)	749, 769
TseFI	GTSAC	2 cut(s)	131, 989
TseI	GCWGC	2 cut(s)	261, 451
Tsp45I	GTSAC	2 cut(s)	131, 989
TspDTI	ATGAA	1 cut(s)	1010
TspGWI	ACGGA	2 cut(s)	460, 526
TspRI	CASTG	2 cut(s)	749, 769
VpaK11BI	GGWCC	2 cut(s)	607, 965
XapI	RAATTY	1 cut(s)	1016
XspI	CTAG	1 cut(s)	932

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.