pycom15g16650

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005364
Physical Location & Seq
Reverse (-)
11688148 .. 11689271
1124 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1029 bp
ATGATGATGGAGGAGAGCAACAACGTCATCGCACCGTTCGTTTTGAAGACTTACCAGATGGTTAACGATCCAACGACGGACAATCTAATCACATGGGGAAGAGCCAACAACAGCTTCATCGTCGTTGACCCTCTGGTCTTCTCCCAGAGACTGTTACCCGCTTACTTCAAGCACAACAATTTCTCCAGCTTCGTCCGCCAGCTCAACACCTATGGATTTCGAAAGGTCGATCCAGATAAGTGGGAGTTCGCGAACGAGTGGTTTCTACGAGGACAGACGCATTTGCTGAGAAACGTGGCGAGGAGAAAACAAATGGACAAGAGTTCTAACTCGAAATCGCTTTTTTGTAATTCGAATTCGAATTTCTTACCAACGAAGCACGAGGAGCTCAACGACGAAGATATATTTATGGAGATTTCGCGGTTGAAGCAGGAGCAGAAGGCGCTGGAGGAAGAAATCGGGCATATGAACCGGCGGCTGGAGACGACGGAGCGACGGCCGCAGCAAATGATGGCGTTTCTACACAAAGTGGCGGAGGACCCGGAGATTCTGCCACGTATGATGCTCGAGAAGGACCGCGCGACGACGGCGCAGTTGGGGGAGAAGAAACGGCGGGTTATGATGATAGCATTGACGTCGTCGTCGTCATCGGGGATGGGGGCCACCACTTCCGTTAGAACTGAGGACGAAGATGATGGAACCGTAGGGGTAATTTCGTCATCTCCTGAAGCAGGTTTTGAGATGGAGAGTTTTAATCGGTATTCCACGTCACCGGAGGTCCAGACGGCGTCTGACTGGTTAAGGCAGAGGCGGTTCGTGGATCAAGTTCGCGCTGTACAAAACCCGAATAATATGAATCCGACGGCGTCGGGTCATGGGGTGGAGAATATCCGGAATAGTAGTAGTTCCGGGTACGGGTATGGGAATGGGAATGGTGGCGGCCCTGGCGGGGAAGTGGGATACTTTACGGAGGTGGAAGCTAGTCCGCCACCGCCTTATCCATTTTCGCTACTGGAGGGAGGCTTTTAG

Protein Analysis

343

Amino Acids

38.71

Weight (kDa)

5.67

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 13 - 102 4.2e-28 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 134, 640
AatII GACGTC 1 cut(s) 638
Acc36I ACCTGC 1 cut(s) 722
AccII CGCG 5 cut(s) 251, 421, 579, 581, 831
AccIII TCCGGA 1 cut(s) 891
AclWI GGATC 3 cut(s) 62, 224, 828
AcoI YGGCCR 1 cut(s) 497
AcsI RAATTY 2 cut(s) 355, 361
AcuI CTGAAG 1 cut(s) 747
AcyI GRCGYC 3 cut(s) 635, 788, 866
AdeI CACNNNGTG 1 cut(s) 529
AfaI GTAC 2 cut(s) 837, 914
AfiI CCNNNNNNNGG 4 cut(s) 478, 731, 915, 949
AgsI TTSAA 3 cut(s) 46, 169, 427
AjiI CACGTC 1 cut(s) 768
AjnI CCWGG 1 cut(s) 943
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AluBI AGCT 5 cut(s) 114, 189, 202, 388, 980
AluI AGCT 5 cut(s) 114, 189, 202, 388, 980
Alw21I GWGCWC 1 cut(s) 390
Alw26I GTCTC 2 cut(s) 142, 476
AlwI GGATC 3 cut(s) 62, 224, 828
AlwNI CAGNNNCTG 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 566
Aor13HI TCCGGA 1 cut(s) 891
AoxI GGCC 3 cut(s) 497, 660, 940
ApeKI GCWGC 1 cut(s) 502
ApoI RAATTY 2 cut(s) 355, 361
AspLEI GCGC 4 cut(s) 445, 581, 592, 833
AspS9I GGNCC 5 cut(s) 538, 574, 660, 778, 941
AsuC2I CCSGG 2 cut(s) 542, 910
AsuHPI GGTGA 1 cut(s) 762
AsuII TTCGAA 3 cut(s) 220, 353, 359
AvaI CYCGRG 1 cut(s) 566
AvaII GGWCC 3 cut(s) 538, 574, 778
BanII GRGCYC 1 cut(s) 390
BauI CACGAG 1 cut(s) 380
BbsI GAAGAC 2 cut(s) 53, 130
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 514
BccI CCATC 5 cut(s) 52, 505, 649, 689, 736
BceAI ACGGC 5 cut(s) 512, 603, 626, 801, 879
BcgI CGANNNNNNTGC 2 cut(s) 618, 652
BciT130I CCWGG 1 cut(s) 945
BciVI GTATCC 1 cut(s) 953
BcnI CCSGG 2 cut(s) 542, 910
BcoDI GTCTC 2 cut(s) 142, 476
BfaI CTAG 1 cut(s) 981
BfoI RGCGCY 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 722
BfuI GTATCC 1 cut(s) 953
BisI GCNGC 4 cut(s) 476, 500, 503, 940
BlsI GCNGC 4 cut(s) 477, 501, 504, 941
Bme1390I CCNGG 3 cut(s) 542, 910, 945
Bme18I GGWCC 3 cut(s) 538, 574, 778
BmeT110I CYCGRG 1 cut(s) 566
BmgBI CACGTC 1 cut(s) 768
BmgT120I GGNCC 5 cut(s) 538, 574, 660, 778, 941
BmiI GGNNCC 3 cut(s) 540, 661, 700
BmrFI CCNGG 3 cut(s) 542, 910, 945
BmsI GCATC 1 cut(s) 552
BpiI GAAGAC 2 cut(s) 53, 130
BpmI CTGGAG 3 cut(s) 169, 467, 500
Bpu14I TTCGAA 3 cut(s) 220, 353, 359
BpuMI CCSGG 2 cut(s) 542, 910
BsaAI YACGTR 1 cut(s) 557
BsaHI GRCGYC 3 cut(s) 635, 788, 866
BsaJI CCNNGG 1 cut(s) 943
BsaWI WCCGGW 2 cut(s) 772, 891
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 4 cut(s) 478, 731, 915, 949
Bse118I RCCGGY 1 cut(s) 471
Bse1I ACTGG 2 cut(s) 800, 1017
BseAI TCCGGA 1 cut(s) 891
BseBI CCWGG 1 cut(s) 945
BseDI CCNNGG 1 cut(s) 943
BseGI GGATG 1 cut(s) 660
BseLI CCNNNNNNNGG 4 cut(s) 478, 731, 915, 949
BseMII CTCAG 2 cut(s) 278, 672
BseNI ACTGG 2 cut(s) 800, 1017
BseRI GAGGAG 3 cut(s) 26, 316, 398
BseX3I CGGCCG 1 cut(s) 497
BseXI GCAGC 1 cut(s) 514
Bsh1236I CGCG 5 cut(s) 251, 421, 579, 581, 831
Bsh1285I CGRYCG 1 cut(s) 500
BshFI GGCC 3 cut(s) 499, 662, 942
BsiEI CGRYCG 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 1 cut(s) 566
BsiSI CCGG 5 cut(s) 472, 542, 773, 892, 909
BslI CCNNNNNNNGG 4 cut(s) 478, 731, 915, 949
BsmAI GTCTC 2 cut(s) 142, 476
BsmBI CGTCTC 1 cut(s) 476
BsnI GGCC 3 cut(s) 499, 662, 942
BsoBI CYCGRG 1 cut(s) 566
Bsp119I TTCGAA 3 cut(s) 220, 353, 359
Bsp1286I GDGCHC 1 cut(s) 390
Bsp13I TCCGGA 1 cut(s) 891
Bsp1407I TGTACA 1 cut(s) 835
Bsp143I GATC 3 cut(s) 67, 229, 820
Bsp68I TCGCGA 1 cut(s) 251
BspANI GGCC 3 cut(s) 499, 662, 942
BspCNI CTCAG 2 cut(s) 279, 673
BspEI TCCGGA 1 cut(s) 891
BspFNI CGCG 5 cut(s) 251, 421, 579, 581, 831
BspLI GGNNCC 3 cut(s) 540, 661, 700
BspMI ACCTGC 1 cut(s) 722
BspPI GGATC 3 cut(s) 62, 224, 828
BspQI GCTCTTC 1 cut(s) 94
BspT104I TTCGAA 3 cut(s) 220, 353, 359
BsrFI RCCGGY 1 cut(s) 471
BsrGI TGTACA 1 cut(s) 835
BsrI ACTGG 2 cut(s) 800, 1017
BssAI RCCGGY 1 cut(s) 471
BssECI CCNNGG 1 cut(s) 943
BssMI GATC 3 cut(s) 67, 229, 820
BssNI GRCGYC 3 cut(s) 635, 788, 866
BssSI CACGAG 1 cut(s) 380
Bst2BI CACGAG 1 cut(s) 380
Bst2UI CCWGG 1 cut(s) 945
Bst4CI ACNGT 3 cut(s) 36, 153, 703
Bst6I CTCTTC 1 cut(s) 94
BstACI GRCGYC 3 cut(s) 635, 788, 866
BstAUI TGTACA 1 cut(s) 835
BstBAI YACGTR 1 cut(s) 557
BstBI TTCGAA 3 cut(s) 220, 353, 359
BstC8I GCNNGC 1 cut(s) 200
BstDEI CTNAG 2 cut(s) 287, 681
BstENI CCTNNNNNAGG 1 cut(s) 729
BstF5I GGATG 1 cut(s) 660
BstFNI CGCG 5 cut(s) 251, 421, 579, 581, 831
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 4 cut(s) 445, 581, 592, 833
BstKTI GATC 3 cut(s) 70, 232, 823
BstMAI GTCTC 2 cut(s) 142, 476
BstMBI GATC 3 cut(s) 67, 229, 820
BstMCI CGRYCG 1 cut(s) 500
BstMWI GCNNNNNNNGC 7 cut(s) 195, 385, 427, 442, 499, 587, 945
BstNI CCWGG 1 cut(s) 945
BstSCI CCNGG 3 cut(s) 540, 908, 943
BstUI CGCG 5 cut(s) 251, 421, 579, 581, 831
BstV1I GCAGC 1 cut(s) 514
BstV2I GAAGAC 2 cut(s) 53, 130
BstXI CCANNNNNNTGG 1 cut(s) 240
BstZI CGGCCG 1 cut(s) 497
BsuI GTATCC 1 cut(s) 953
BsuRI GGCC 3 cut(s) 499, 662, 942
BtgZI GCGATG 1 cut(s) 13
BtrI CACGTC 1 cut(s) 768
BtsCI GGATG 1 cut(s) 660
BtuMI TCGCGA 1 cut(s) 251
BveI ACCTGC 1 cut(s) 722
Cac8I GCNNGC 1 cut(s) 200
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 4 cut(s) 445, 581, 592, 833
Cfr10I RCCGGY 1 cut(s) 471
Cfr13I GGNCC 5 cut(s) 538, 574, 660, 778, 941
CseI GACGC 3 cut(s) 286, 777, 855
Csp6I GTAC 2 cut(s) 836, 913
CviAII CATG 2 cut(s) 93, 875
CviQI GTAC 2 cut(s) 836, 913
DdeI CTNAG 2 cut(s) 287, 681
DpnI GATC 3 cut(s) 69, 231, 822
DpnII GATC 3 cut(s) 67, 229, 820
DraIII CACNNNGTG 1 cut(s) 529
DrdI GACNNNNNNGTC 2 cut(s) 134, 640
DseDI GACNNNNNNGTC 2 cut(s) 134, 640
EaeI YGGCCR 1 cut(s) 497
EagI CGGCCG 1 cut(s) 497
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
EciI GGCGGA 3 cut(s) 185, 548, 975
Ecl136II GAGCTC 1 cut(s) 388
EclXI CGGCCG 1 cut(s) 497
Eco24I GRGCYC 1 cut(s) 390
Eco47I GGWCC 3 cut(s) 538, 574, 778
Eco52I CGGCCG 1 cut(s) 497
Eco53kI GAGCTC 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 747
Eco88I CYCGRG 1 cut(s) 566
EcoICRI GAGCTC 1 cut(s) 388
EcoNI CCTNNNNNAGG 1 cut(s) 729
EcoO109I RGGNCCY 1 cut(s) 538
EcoRI GAATTC 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 943
EcoT38I GRGCYC 1 cut(s) 390
Esp3I CGTCTC 1 cut(s) 476
FaeI CATG 2 cut(s) 96, 878
FatI CATG 2 cut(s) 92, 874
FauI CCCGC 3 cut(s) 166, 606, 941
FauNDI CATATG 1 cut(s) 465
Fnu4HI GCNGC 4 cut(s) 476, 500, 503, 940
FokI GGATG 1 cut(s) 667
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 4 cut(s) 476, 500, 503, 940
FspBI CTAG 1 cut(s) 981
GlaI GCGC 4 cut(s) 444, 580, 591, 832
GluI GCNGC 4 cut(s) 476, 500, 503, 940
GsuI CTGGAG 3 cut(s) 169, 467, 500
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 3 cut(s) 499, 662, 942
HapII CCGG 5 cut(s) 472, 542, 773, 892, 909
HgaI GACGC 3 cut(s) 286, 777, 855
HhaI GCGC 4 cut(s) 445, 581, 592, 833
Hin1I GRCGYC 3 cut(s) 635, 788, 866
Hin1II CATG 2 cut(s) 96, 878
Hin6I GCGC 4 cut(s) 443, 579, 590, 831
HinP1I GCGC 4 cut(s) 443, 579, 590, 831
HincII GTYRAC 2 cut(s) 64, 127
HindII GTYRAC 2 cut(s) 64, 127
HinfI GANTC 2 cut(s) 547, 856
HpaI GTTAAC 1 cut(s) 64
HpaII CCGG 5 cut(s) 472, 542, 773, 892, 909
HphI GGTGA 1 cut(s) 762
Hpy166II GTNNAC 2 cut(s) 64, 127
Hpy188I TCNGA 2 cut(s) 793, 861
Hpy188III TCNNGA 6 cut(s) 233, 250, 568, 725, 781, 892
Hpy8I GTNNAC 2 cut(s) 64, 127
HpyAV CCTTC 2 cut(s) 433, 565
HpyCH4III ACNGT 3 cut(s) 36, 153, 703
HpyCH4IV ACGT 5 cut(s) 24, 294, 556, 635, 767
HpyF10VI GCNNNNNNNGC 7 cut(s) 195, 385, 427, 442, 499, 587, 945
HpyF3I CTNAG 2 cut(s) 287, 681
HpySE526I ACGT 5 cut(s) 24, 294, 556, 635, 767
Hsp92I GRCGYC 3 cut(s) 635, 788, 866
Hsp92II CATG 2 cut(s) 96, 878
HspAI GCGC 4 cut(s) 443, 579, 590, 831
Kpn2I TCCGGA 1 cut(s) 891
KspAI GTTAAC 1 cut(s) 64
Kzo9I GATC 3 cut(s) 67, 229, 820
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 3 cut(s) 385, 433, 490
Lsp1109I GCAGC 1 cut(s) 514
LweI GCATC 1 cut(s) 552
MaeI CTAG 1 cut(s) 981
MaeII ACGT 5 cut(s) 24, 294, 556, 635, 767
MaeIII GTNAC 2 cut(s) 153, 768
MalI GATC 3 cut(s) 69, 231, 822
MboI GATC 3 cut(s) 67, 229, 820
MboII GAAGA 7 cut(s) 58, 111, 130, 410, 464, 616, 701
MhlI GDGCHC 1 cut(s) 390
MluCI AATT 5 cut(s) 178, 349, 355, 361, 711
MmeI TCCRAC 2 cut(s) 95, 884
MroI TCCGGA 1 cut(s) 891
MseI TTAA 3 cut(s) 63, 753, 800
MspI CCGG 5 cut(s) 472, 542, 773, 892, 909
MspR9I CCNGG 3 cut(s) 542, 910, 945
MvaI CCWGG 1 cut(s) 945
MvnI CGCG 5 cut(s) 251, 421, 579, 581, 831
MwoI GCNNNNNNNGC 7 cut(s) 195, 385, 427, 442, 499, 587, 945
NciI CCSGG 2 cut(s) 542, 910
NdeI CATATG 1 cut(s) 465
NdeII GATC 3 cut(s) 67, 229, 820
NlaIII CATG 2 cut(s) 96, 878
NlaIV GGNNCC 3 cut(s) 540, 661, 700
NmuCI GTSAC 1 cut(s) 768
NruI TCGCGA 1 cut(s) 251
NspV TTCGAA 3 cut(s) 220, 353, 359
PaeR7I CTCGAG 1 cut(s) 566
PciSI GCTCTTC 1 cut(s) 94
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 641
PfeI GAWTC 2 cut(s) 547, 856
PflFI GACNNNGTC 3 cut(s) 637, 787, 865
PkrI GCNGC 4 cut(s) 477, 501, 504, 941
Ppu21I YACGTR 1 cut(s) 557
PpuMI RGGWCCY 1 cut(s) 538
Psp124BI GAGCTC 1 cut(s) 390
Psp5II RGGWCCY 1 cut(s) 538
Psp6I CCWGG 1 cut(s) 943
PspGI CCWGG 1 cut(s) 943
PspN4I GGNNCC 3 cut(s) 540, 661, 700
PspPI GGNCC 5 cut(s) 538, 574, 660, 778, 941
PspPPI RGGWCCY 1 cut(s) 538
PstNI CAGNNNCTG 1 cut(s) 151
PsyI GACNNNGTC 3 cut(s) 637, 787, 865
RruI TCGCGA 1 cut(s) 251
RsaI GTAC 2 cut(s) 837, 914
RsaNI GTAC 2 cut(s) 836, 913
SacI GAGCTC 1 cut(s) 390
SapI GCTCTTC 1 cut(s) 94
SaqAI TTAA 3 cut(s) 63, 753, 800
SatI GCNGC 4 cut(s) 476, 500, 503, 940
Sau3AI GATC 3 cut(s) 67, 229, 820
Sau96I GGNCC 5 cut(s) 538, 574, 660, 778, 941
ScrFI CCNGG 3 cut(s) 542, 910, 945
SduI GDGCHC 1 cut(s) 390
SfaNI GCATC 1 cut(s) 552
Sfr274I CTCGAG 1 cut(s) 566
SfuI TTCGAA 3 cut(s) 220, 353, 359
SinI GGWCC 3 cut(s) 538, 574, 778
SlaI CTCGAG 1 cut(s) 566
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 5 cut(s) 178, 349, 355, 361, 711
SspMI CTAG 1 cut(s) 981
SstI GAGCTC 1 cut(s) 390
StyD4I CCNGG 3 cut(s) 540, 908, 943
TaaI ACNGT 3 cut(s) 36, 153, 703
TaiI ACGT 5 cut(s) 27, 297, 559, 638, 770
TaqI TCGA 6 cut(s) 220, 228, 332, 353, 359, 567
TasI AATT 5 cut(s) 178, 349, 355, 361, 711
TatI WGTACW 1 cut(s) 835
TauI GCSGC 3 cut(s) 478, 502, 942
TfiI GAWTC 2 cut(s) 547, 856
Tru1I TTAA 3 cut(s) 63, 753, 800
Tru9I TTAA 3 cut(s) 63, 753, 800
TseFI GTSAC 1 cut(s) 768
TseI GCWGC 1 cut(s) 502
Tsp45I GTSAC 1 cut(s) 768
TspDTI ATGAA 3 cut(s) 106, 482, 869
TspGWI ACGGA 4 cut(s) 92, 503, 661, 983
Tth111I GACNNNGTC 3 cut(s) 637, 787, 865
VpaK11BI GGWCC 3 cut(s) 538, 574, 778
XagI CCTNNNNNAGG 1 cut(s) 729
XapI RAATTY 2 cut(s) 355, 361
XhoI CTCGAG 1 cut(s) 566
XspI CTAG 1 cut(s) 981
ZraI GACGTC 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.