pycom15g27610

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005190
Physical Location & Seq
Forward (+)
23074283 .. 23075816
1534 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1236 bp
ATGCCAACTGCAGAACACAGACATTGTGTTAGACATTTGCATAATAATTTTAGAAGTGCAGGTCACACTGGACTTGCCTTAAAACAGAGATTGTGGGCAGCAGCAAGAGCAACTACTATACCTGCTTTTGAAGCTGAAATTGAGGTGATGAATAATCAATCTGATAAGGCAGTGGAATGGCTAGCTGATAAGCCACCTTGCCATTGGAGCAGATCTTATTTCAGAACATCGGTAAAGTGTGATTTGTTATTGAATAACATGTGTGAGTCATTTAATTCTGCAATTTTAGATGCAAGAGATAAACCGATTATTACGATGTTACAAAGGATTCAAAAATATTTGATGTTGAGAATGGCTAGGCTAAGAGAGGCTAAGTGGACGCAACAAGTTGGACCTAGAATTTTAAAGATTGTTGAAAAAAATCAAATGGATAGTTTCAGTTGTTTTGCCAATTATTCAGGGAATGGTCAATATCAAGTGCATACTTTGGTTGGTAGTATGTTTGCAGTAGATTTGGAAAGGCACACTTGCTCTTGTAGGAAGTGGCAGCTATGTGGCATTCCTTGTCCCCATGCTATATCCGCAATTGCTAGGAAGGAGGCTAGTCCACAATTGTTTGTTCACTCACTTTACAAAAGGCCAGCTTATGATAGGTGTTATGAAGGGTATATTGCTCCTATGCCTGAAAAAGAACAGTGGAAGAGAACCTACCTTAGGCCTATTAAACCTCCTTTTTACCACAAACAACCTGGAAGACCTAAAGGCAAAAGAACAAAAGCACCAGATGAGATAAAGAAGGGGGCAACAAAGCTAAGGAAGTATGATGTTGTAATGCATTGCCAAACCTGTGGAGAGGAAGGACACAACAAAACAAGTTGTCCACAAGGGCTACTACAATCACAACAAGGTTTTGGACTCACAAAAGAGAGTCATGGGCGAACAGTCGGTACAAGCATATCTAAAGGGTCTAGCTCAAATGCAAAAGGACCTAAAACGCCAAAAGGAAATAACACAATAAGTGGTCTTAACTCAAAAGCAAAAGGATTGGCCACAGTTGCAAAGGAAATTGGTGCACATCACCACTCATGGGCTGCTCCATCTCAAACTAGGCCACAAGGAACTTCTAAACTACATGTGAACCGAGGTGGTCGTGTGAATCAAGTCACAAGGAAGGCTAATGAAATGGCATCACAACCCCAACATGTTGCCAAAAAAGCAAGACCATGGAGAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

412

Amino Acids

46.31

Weight (kDa)

10.01

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIM PF04434 170 - 197 3.6e-08 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 50, 130
AciI CCGC 1 cut(s) 582
AcoI YGGCCR 1 cut(s) 1047
AcsI RAATTY 1 cut(s) 399
AfaI GTAC 1 cut(s) 949
AfiI CCNNNNNNNGG 2 cut(s) 714, 1087
AflIII ACRYGT 3 cut(s) 258, 1132, 1201
AgsI TTSAA 4 cut(s) 131, 253, 332, 416
AjnI CCWGG 1 cut(s) 748
AluBI AGCT 6 cut(s) 134, 185, 550, 644, 811, 972
AluI AGCT 6 cut(s) 134, 185, 550, 644, 811, 972
Alw21I GWGCWC 1 cut(s) 1075
Alw44I GTGCAC 1 cut(s) 1071
AoxI GGCC 4 cut(s) 638, 716, 1047, 1109
ApaLI GTGCAC 1 cut(s) 1071
ApeKI GCWGC 4 cut(s) 98, 101, 547, 1091
ApoI RAATTY 1 cut(s) 399
AspS9I GGNCC 2 cut(s) 392, 986
AsuHPI GGTGA 2 cut(s) 157, 1070
AsuNHI GCTAGC 1 cut(s) 181
AvaII GGWCC 2 cut(s) 392, 986
AxyI CCTNAGG 1 cut(s) 713
BaeGI GKGCMC 1 cut(s) 1075
BalI TGGCCA 1 cut(s) 1049
BarI GAAGNNNNNNTAC 2 cut(s) 692, 724
BbsI GAAGAC 1 cut(s) 760
Bbv12I GWGCWC 1 cut(s) 1075
BbvI GCAGC 4 cut(s) 110, 113, 559, 1078
BccI CCATC 1 cut(s) 1105
BciT130I CCWGG 1 cut(s) 750
BfaI CTAG 7 cut(s) 182, 357, 396, 591, 603, 969, 1107
BfmI CTRYAG 1 cut(s) 9
BfuAI ACCTGC 2 cut(s) 50, 130
BglII AGATCT 1 cut(s) 212
BisI GCNGC 4 cut(s) 99, 102, 548, 1092
BlsI GCNGC 4 cut(s) 100, 103, 549, 1093
Bme1390I CCNGG 1 cut(s) 750
Bme18I GGWCC 2 cut(s) 392, 986
BmgT120I GGNCC 2 cut(s) 392, 986
BmrFI CCNGG 1 cut(s) 750
BmsI GCATC 2 cut(s) 280, 1196
BmtI GCTAGC 1 cut(s) 185
BpiI GAAGAC 1 cut(s) 760
Bpu10I CCTNAGC 1 cut(s) 812
BsaJI CCNNGG 2 cut(s) 1141, 1223
Bsc4I CCNNNNNNNGG 2 cut(s) 714, 1087
Bse1I ACTGG 1 cut(s) 73
Bse21I CCTNAGG 1 cut(s) 713
Bse3DI GCAATG 1 cut(s) 835
BseBI CCWGG 1 cut(s) 750
BseDI CCNNGG 2 cut(s) 1141, 1223
BseLI CCNNNNNNNGG 2 cut(s) 714, 1087
BseMI GCAATG 1 cut(s) 835
BseNI ACTGG 1 cut(s) 73
BseSI GKGCMC 1 cut(s) 1075
BseXI GCAGC 4 cut(s) 110, 113, 559, 1078
BsgI GTGCAG 1 cut(s) 78
BshFI GGCC 4 cut(s) 640, 718, 1049, 1111
BsiHKAI GWGCWC 1 cut(s) 1075
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 2 cut(s) 714, 1087
BsmFI GGGAC 1 cut(s) 552
BsmI GAATGC 1 cut(s) 558
BsnI GGCC 4 cut(s) 640, 718, 1049, 1111
Bsp1286I GDGCHC 1 cut(s) 1075
Bsp143I GATC 1 cut(s) 212
Bsp19I CCATGG 1 cut(s) 1223
BspACI CCGC 1 cut(s) 582
BspANI GGCC 4 cut(s) 640, 718, 1049, 1111
BspMAI CTGCAG 1 cut(s) 13
BspMI ACCTGC 2 cut(s) 50, 130
BspOI GCTAGC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 835
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 2 cut(s) 1141, 1223
BssMI GATC 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 1223
Bst2UI CCWGG 1 cut(s) 750
Bst4CI ACNGT 3 cut(s) 696, 943, 1054
Bst6I CTCTTC 1 cut(s) 695
BstC8I GCNNGC 2 cut(s) 183, 642
BstDEI CTNAG 4 cut(s) 362, 372, 713, 812
BstDSI CCRYGG 1 cut(s) 1223
BstENI CCTNNNNNAGG 1 cut(s) 712
BstKTI GATC 1 cut(s) 215
BstMBI GATC 1 cut(s) 212
BstMWI GCNNNNNNNGC 6 cut(s) 107, 131, 207, 581, 1055, 1214
BstNI CCWGG 1 cut(s) 750
BstNSI RCATGY 3 cut(s) 262, 1136, 1205
BstSCI CCNGG 1 cut(s) 748
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 1 cut(s) 1075
BstV1I GCAGC 4 cut(s) 110, 113, 559, 1078
BstV2I GAAGAC 1 cut(s) 760
BstX2I RGATCY 1 cut(s) 212
BstXI CCANNNNNNTGG 1 cut(s) 848
BstYI RGATCY 1 cut(s) 212
Bsu36I CCTNAGG 1 cut(s) 713
BsuRI GGCC 4 cut(s) 640, 718, 1049, 1111
BtgI CCRYGG 1 cut(s) 1223
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 3 cut(s) 66, 177, 701
BveI ACCTGC 2 cut(s) 50, 130
Cac8I GCNNGC 2 cut(s) 183, 642
Cfr13I GGNCC 2 cut(s) 392, 986
CseI GACGC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 948
CviAII CATG 7 cut(s) 259, 572, 932, 1086, 1133, 1202, 1224
CviQI GTAC 1 cut(s) 948
DdeI CTNAG 4 cut(s) 362, 372, 713, 812
DpnI GATC 1 cut(s) 214
DpnII GATC 1 cut(s) 212
DraI TTTAAA 1 cut(s) 405
EaeI YGGCCR 1 cut(s) 1047
Eam1104I CTCTTC 1 cut(s) 695
EarI CTCTTC 1 cut(s) 695
Eco130I CCWWGG 1 cut(s) 1223
Eco147I AGGCCT 1 cut(s) 718
Eco47I GGWCC 2 cut(s) 392, 986
Eco81I CCTNAGG 1 cut(s) 713
EcoNI CCTNNNNNAGG 1 cut(s) 712
EcoO109I RGGNCCY 1 cut(s) 986
EcoRII CCWGG 1 cut(s) 748
EcoT14I CCWWGG 1 cut(s) 1223
EcoT22I ATGCAT 1 cut(s) 837
ErhI CCWWGG 1 cut(s) 1223
FaeI CATG 7 cut(s) 262, 575, 935, 1089, 1136, 1205, 1227
FalI AAGNNNNNCTT 4 cut(s) 511, 543, 628, 660
FaqI GGGAC 1 cut(s) 552
FatI CATG 7 cut(s) 258, 571, 931, 1085, 1132, 1201, 1223
Fnu4HI GCNGC 4 cut(s) 99, 102, 548, 1092
Fsp4HI GCNGC 4 cut(s) 99, 102, 548, 1092
FspBI CTAG 7 cut(s) 182, 357, 396, 591, 603, 969, 1107
GluI GCNGC 4 cut(s) 99, 102, 548, 1092
HaeIII GGCC 4 cut(s) 640, 718, 1049, 1111
HgaI GACGC 1 cut(s) 388
Hin1II CATG 7 cut(s) 262, 575, 935, 1089, 1136, 1205, 1227
HinfI GANTC 5 cut(s) 266, 328, 915, 928, 1156
HphI GGTGA 2 cut(s) 157, 1070
Hpy166II GTNNAC 6 cut(s) 378, 608, 622, 881, 1073, 1138
Hpy188I TCNGA 2 cut(s) 163, 224
Hpy8I GTNNAC 6 cut(s) 378, 608, 622, 881, 1073, 1138
HpyAV CCTTC 5 cut(s) 589, 656, 790, 851, 1165
HpyCH4III ACNGT 3 cut(s) 696, 943, 1054
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 131, 207, 581, 1055, 1214
HpyF3I CTNAG 4 cut(s) 362, 372, 713, 812
Hsp92II CATG 7 cut(s) 262, 575, 935, 1089, 1136, 1205, 1227
Kzo9I GATC 1 cut(s) 212
LmnI GCTCC 3 cut(s) 207, 679, 1099
Lsp1109I GCAGC 4 cut(s) 110, 113, 559, 1078
LweI GCATC 2 cut(s) 280, 1196
MaeI CTAG 7 cut(s) 182, 357, 396, 591, 603, 969, 1107
MaeIII GTNAC 3 cut(s) 62, 318, 1162
MalI GATC 1 cut(s) 214
MboI GATC 1 cut(s) 212
MboII GAAGA 2 cut(s) 712, 765
MfeI CAATTG 2 cut(s) 585, 611
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 1 cut(s) 1075
MlsI TGGCCA 1 cut(s) 1049
MluCI AATT 9 cut(s) 46, 138, 274, 282, 399, 451, 585, 611, 1065
MluNI TGGCCA 1 cut(s) 1049
MlyI GAGTC 3 cut(s) 275, 909, 937
MmeI TCCRAC 1 cut(s) 370
MnlI CCTC 6 cut(s) 136, 361, 592, 738, 847, 1136
Mox20I TGGCCA 1 cut(s) 1049
Mph1103I ATGCAT 1 cut(s) 837
MscI TGGCCA 1 cut(s) 1049
MseI TTAA 5 cut(s) 80, 273, 404, 723, 1026
Msp20I TGGCCA 1 cut(s) 1049
MspR9I CCNGG 1 cut(s) 750
MunI CAATTG 2 cut(s) 585, 611
Mva1269I GAATGC 1 cut(s) 558
MvaI CCWGG 1 cut(s) 750
MwoI GCNNNNNNNGC 6 cut(s) 107, 131, 207, 581, 1055, 1214
NcoI CCATGG 1 cut(s) 1223
NdeII GATC 1 cut(s) 212
NheI GCTAGC 1 cut(s) 181
NlaIII CATG 7 cut(s) 262, 575, 935, 1089, 1136, 1205, 1227
NmuCI GTSAC 2 cut(s) 62, 1162
NsiI ATGCAT 1 cut(s) 837
NspI RCATGY 3 cut(s) 262, 1136, 1205
PceI AGGCCT 1 cut(s) 718
PciI ACATGT 3 cut(s) 258, 1132, 1201
PctI GAATGC 1 cut(s) 558
PfeI GAWTC 2 cut(s) 328, 1156
PkrI GCNGC 4 cut(s) 100, 103, 549, 1093
PleI GAGTC 3 cut(s) 274, 909, 936
PpsI GAGTC 3 cut(s) 274, 909, 936
PpuMI RGGWCCY 1 cut(s) 986
PscI ACATGT 3 cut(s) 258, 1132, 1201
Psp5II RGGWCCY 1 cut(s) 986
Psp6I CCWGG 1 cut(s) 748
PspGI CCWGG 1 cut(s) 748
PspPI GGNCC 2 cut(s) 392, 986
PspPPI RGGWCCY 1 cut(s) 986
PsrI GAACNNNNNNTAC 2 cut(s) 931, 963
PstI CTGCAG 1 cut(s) 13
PsuI RGATCY 1 cut(s) 212
RsaI GTAC 1 cut(s) 949
RsaNI GTAC 1 cut(s) 948
SaqAI TTAA 5 cut(s) 80, 273, 404, 723, 1026
SatI GCNGC 4 cut(s) 99, 102, 548, 1092
Sau3AI GATC 1 cut(s) 212
Sau96I GGNCC 2 cut(s) 392, 986
SchI GAGTC 3 cut(s) 275, 909, 937
ScrFI CCNGG 1 cut(s) 750
SduI GDGCHC 1 cut(s) 1075
SfaNI GCATC 2 cut(s) 280, 1196
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 2 cut(s) 392, 986
Sse9I AATT 9 cut(s) 46, 138, 274, 282, 399, 451, 585, 611, 1065
SseBI AGGCCT 1 cut(s) 718
SsiI CCGC 1 cut(s) 582
SspI AATATT 1 cut(s) 338
SspMI CTAG 7 cut(s) 182, 357, 396, 591, 603, 969, 1107
StuI AGGCCT 1 cut(s) 718
StyD4I CCNGG 1 cut(s) 748
StyI CCWWGG 1 cut(s) 1223
TaaI ACNGT 3 cut(s) 696, 943, 1054
TasI AATT 9 cut(s) 46, 138, 274, 282, 399, 451, 585, 611, 1065
TfiI GAWTC 2 cut(s) 328, 1156
Tru1I TTAA 5 cut(s) 80, 273, 404, 723, 1026
Tru9I TTAA 5 cut(s) 80, 273, 404, 723, 1026
TscAI CASTG 3 cut(s) 73, 177, 701
TseFI GTSAC 2 cut(s) 62, 1162
TseI GCWGC 4 cut(s) 98, 101, 547, 1091
Tsp45I GTSAC 2 cut(s) 62, 1162
TspDTI ATGAA 3 cut(s) 164, 675, 1194
TspRI CASTG 3 cut(s) 73, 177, 701
VneI GTGCAC 1 cut(s) 1071
VpaK11BI GGWCC 2 cut(s) 392, 986
XagI CCTNNNNNAGG 1 cut(s) 712
XapI RAATTY 1 cut(s) 399
XceI RCATGY 3 cut(s) 262, 1136, 1205
XcmI CCANNNNNNNNNTGG 2 cut(s) 201, 746
XspI CTAG 7 cut(s) 182, 357, 396, 591, 603, 969, 1107
Zsp2I ATGCAT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.