Rorug02G0169100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005190
Physical Location & Seq
Reverse (-)
15184106 .. 15197044
12939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0169100.1

Sequence Viewer

Length: 3738 bp
ATGGTAATAGTGGGGGAGTTTGTTGGAGGGGCTGCTCTTGGAGCAGCATTCGGCACTCTCTTTGATGTGGTTAAGGAAGCGTTGGAGAAATCTGCATTGTTTAAACCCTTACTCAAGACCATTAAATCCACTCTTGATTCTTTGCAACCGCTGATCCAACAGATAGAAATGCACAATGCTGAATTGAACCTCCCGAACGAGGAAATAGAATCCTGTAAGAAGCAGATGGAGGAGGGCGTTGAGCTCGTTCGAAAGCTATCCAAAGTTTCCAAGTGGAACTACAGGAAGCCTCGTTACACTGATCAACTTGTTCAGTTGGATGGATCCCTTAAGAGAATGTTGGATATAATGAAGGTGCAGGAAGCAAGGGATGTGAAGGAAACCTTGGTTTTGGTGCGGAGTATAGATACTGTGGTCAAGCGACTTGAAGGGAATGGTTTGGTACAGAGTCAATTTGAGATCAACGGTTGTTGTGCAGTGCCTGATGAACAACCTCCTCCGACGATTACAGTTGGATTGGATGCTCCATTGGAGGAACTAAAGAAGAATTTTCTCAAGGATGGGGTGTCGATGCTTGTACTGGCTGGTCCTGGAGGATGTGGGAAAACCACATTGGCAAAAGCCTTCTGTCAAGATCAGGAAGTCAAAGATATATTCAAGAAAAATATCTTCTTTGTTACTGTTTCCAAAACGCCCAACTTGAACCATATTGTTCGAGAACTGTGTCAACTTAAGGGCTTTCAGGTGCCTTTTTTCAATACCGAAGTAACTGCAGTTAGCTGGCTGGAAACATTTCTAAGGGAAACTGTTCAACATCCTCTACTGTTGGTCCTAGATGGTGTTTGGTCTGGATCAGAATCCCTTCTTAACAAGTTCGCGATCAAAATGTCAAATTACAAGATTTTGGTGACATCAAGATTTGCATTTCCAAGATTTGGTTCTGCATATTATTTAGCATCACTGACACATGAAGATGCAATGACTCTTTTTCGTCATTCCGCATCCTTGGGAGATAGGAGCTCTTATATTCCAGAAGATCTTCCAAGAAAGATAGTGGAGCGCTGTAAGGGATTTCCTCCTGCCATTACAATGGTAGGAAGGTCACTTTGCGGGCAGCCTGTTGAGATTTGGCAAAATAGATTGATGGAATGGTCAAGAGGTTCTTCTATTCTTGATTCTGAGAGTGATTTGCTTGACTACCTCAAAAACAACTTAGCTTTCTTGGACAAAGGAGATATGACTATCGTCAAGGAATGTTTCATAGACCTTGGCTCGTTTCCTGAAGACCAGAGAATCCCTGTTGCGGGCCTTATTGATATGTGGGCAGAACTATATGAGCTAGATGAAGACTGCATGTCCATTGCAAATCTCTACGAACTTGCTTCCCAGGGTCTGGCAAATCTTGTATGCACAAGAAAAGATAACTGGGAGGTGGATAGATACTTCAGCGAACACTTTGTCACCCAACACTATATGCTTAGAGAGCTAGCTATCCATCAGACTAGTCAGGAACCAATAGCACAGAGAAAAAGATTGATTATTGACATATGTGGAGACAATCTTCCCAGATGGTGGACAGAACAGAAGTTTCAACCCGTTAAAGCACGCTTGTTATCTATCTCAACTGATGGAGTGTTCTCAACAAAATGGCACAACATGCAACTACCACAGGCTGAGGTTTTAGTACTGAACTTTCAGACAAAGAACTATGCCTTACCCGAATTTGTGGAGAAAATGGATAAACTGAAGGTTCTAATAGTCACAAATTATGGTTTCTTACCTGCTGAATTAAGCAATTTTCAACTCTTGGGGTCTTCATCAAATCTAAAGAGAATCAGATTAGAGCGGATTTCAATTCCTTCTCTAATTAATGACTCTGTACAATTGAAGAGTCTGCAGAAGATATCTTTATATATGTGTAACATTGGCCAAGCTTTTAGCAATTGCTCCATCCAAATTACATATGCATTTCCAAATCTAGTAGAAATGAACATTGATTACTGCAATGATTTGTTGGATTTACCTTACAACCTTTGTGATCTTATTCATCTAAAGAAGCTCAGTATCACAAACTGTCATAAGCTATATGCCTTGCCCGAAGAAATTGGAAAGCTGGTCAATCTACAGGTCTTGAGGCTGAGGTCTTGTATACACTTGTTAGAGTTGCCAAATTCGATCAGAAGCCTCAAAATGCTATACTTTCTGGACATATCTGATTGCTACAGCATGAAGGAATTGCCTGAAGATATTGGAGAGTTGTGCAGTTTGAAAAAGCTCAATATGAGGCAGTGTTCAAAATTGCAGGAGTTGCCCCCATCAGTTTTGTGGCTCAAGTTGTTACAGGAAGTGGTATGTGATGAAAATACAGAAAAATTATGGGAACCTTTCATACCCTATCTCAGAAAGATACACATAAAGGTGGCCAAACAGAATTGGCACCCCTGCTCGGATTGGACTAATCTTGTAGTGACAAGGATGGAAAAGACAGAGGTAGTGGACAACATGGTTGAATCGCAGATAAACACAGTTCAGGCTGACATACAAGCAGAGTTTAAGGGTGAGATAAAGGTGGCCAGAGATAATTTGTTCCCCACCCAGAGTCAGTCTAATCTTGTAGTCACAAGGGTGGAGAAGAGAGAGGTGATGGATAAACCAGTTAAATCACATATTGAGACCATTAAGACAGAGTTACAAGCAGACTTTAAGGCCGAGATAAAAGCACTCAGAGACGAGTTTACAAATAAACTTGATGTCATTACGGATTTACTTGTCCACCTCATACACGGAAAGAATGAGAAGGAATGGGAAGGCAAGGACATTCCAGAACAAATAACAAGTGTTTTCGGAGACTGTGAGAGTGGGGCTGCCAAATCAGGTTTCCGTATGGATAGAAACTCAAAGAAGGTAACACACGAACTTCAAAAGGTCAATCAACTTAGCCCTCTCCCGTCTGAGGAACAGATAGTGAGGCAGTTTAGGGCATGGATCAGTAATCCAAACGCCAGCCGGTGTAACTTGGAAATCTTTGATGCACCTAAAGAGTGGTTTATAGACTTGATGACCTCGGGCACATGGTTGAGTGACTCGCATGTAGATGTGTTCTTGTATGCTGTTCGTAAGAGAGCAAATCTTCAGTCCCAATCTTCCTCTCAGTGTTGGACCATCTTGGATACTGTGTTTTGGAGTTGGATGAATGGGCGATGGGAGGATTTTCAGAACAACTCCAAGTCCTATACGTGGGATTCAGACCTTAGGGACTACCCGCTTGGCAAGTCGCCTAAATATGCTCGAAGATGGAGTGAAGTTGATCATTTGTACCTTCCGATCAACATCAACAACCAGCATTGGGTAGCTGTATGCGCGGACATAATACGAAGAAAGTTGACGGTATACAATTCTATGCGGTCTGCAACTCAAGATGAGTTCATTTCTAACATATTGAAGCCCATGGCGACAATGTTGCCATCGCTACTTGTCCAGTCTGGCTTTTACGAACACAGGCCTGAGCTGTCCCCTCTGACTACTCCGTTTAAGGCGGTACATTTAGCAGACAACATTCCGCAACAGATTGTCAGTGGCGATTGTGGGGTGTTCATGCTCAATTACATAGAGTACTTGAGCTCCGGACGACCATTGAGTTTTGTACAAGCAGACATACAACTATTTAGGGAGAAAATGGCATTGGAGATGTTTGCCTTGACAATGATGGGGTGTTCTCAACAAAATAGAAGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

1245

Amino Acids

142.64

Weight (kDa)

6.06

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 138 1.2e-31 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 175 - 306 3.5e-12 NB-ARC domain
LRR_14 PF23598 657 - 740 3.4e-09 Leucine-rich repeat region
Peptidase_C48 PF02902 1025 - 1231 6.2e-32 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1789
AccB1I GGYRCC 2 cut(s) 745, 2436
AccB7I CCANNNNNTGG 3 cut(s) 935, 1393, 1572
AccBSI CCGCTC 1 cut(s) 1846
AccI GTMKAC 2 cut(s) 2149, 3390
AccII CGCG 2 cut(s) 878, 3362
AccIII TCCGGA 1 cut(s) 3623
AclWI GGATC 5 cut(s) 148, 318, 331, 859, 2993
AcoI YGGCCR 3 cut(s) 1927, 2421, 2571
AcsI RAATTY 3 cut(s) 547, 1721, 2170
AcuI CTGAAG 5 cut(s) 1302, 1429, 1766, 2262, 3116
AfaI GTAC 8 cut(s) 444, 579, 1686, 1881, 3317, 3540, 3614, 3645
AfeI AGCGCT 1 cut(s) 1061
AflII CTTAAG 2 cut(s) 329, 731
AhdI GACNNNNNGTC 1 cut(s) 1354
AhlI ACTAGT 1 cut(s) 1502
AjnI CCWGG 2 cut(s) 589, 1386
AleI CACNNNNGTG 1 cut(s) 2624
Alw21I GWGCWC 3 cut(s) 246, 1022, 3623
Alw26I GTCTC 4 cut(s) 1548, 2666, 2721, 2841
AlwI GGATC 5 cut(s) 148, 318, 331, 859, 2993
AlwNI CAGNNNCTG 2 cut(s) 482, 1393
Ama87I CYCGRG 1 cut(s) 3064
Aor13HI TCCGGA 1 cut(s) 3623
Aor51HI AGCGCT 1 cut(s) 1061
AoxI GGCC 6 cut(s) 1306, 1927, 2421, 2571, 2706, 3500
ApeKI GCWGC 5 cut(s) 32, 44, 1114, 2864, 3732
ApoI RAATTY 3 cut(s) 547, 1721, 2170
ArsI GACNNNNNNTTYG 4 cut(s) 1443, 1475, 3119, 3151
AseI ATTAAT 1 cut(s) 1869
Asp700I GAANNNNTTC 4 cut(s) 792, 807, 861, 1038
AspLEI GCGC 2 cut(s) 1062, 3362
AspS9I GGNCC 4 cut(s) 587, 829, 1306, 3159
AsuHPI GGTGA 4 cut(s) 919, 1453, 2570, 2653
AsuII TTCGAA 1 cut(s) 250
AsuNHI GCTAGC 1 cut(s) 1486
AvaI CYCGRG 1 cut(s) 3064
AvaII GGWCC 3 cut(s) 587, 829, 3159
AxyI CCTNAGG 1 cut(s) 3251
BaeGI GKGCMC 1 cut(s) 3071
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BalI TGGCCA 3 cut(s) 1929, 2423, 2573
BamHI GGATCC 1 cut(s) 323
BanI GGYRCC 2 cut(s) 745, 2436
BanII GRGCYC 3 cut(s) 246, 1022, 3623
BarI GAAGNNNNNNTAC 2 cut(s) 278, 310
BbsI GAAGAC 3 cut(s) 1290, 1353, 1806
Bbv12I GWGCWC 3 cut(s) 246, 1022, 3623
BbvCI CCTCAGC 2 cut(s) 1674, 2138
BbvI GCAGC 5 cut(s) 19, 56, 1126, 2851, 3719
BcgI CGANNNNNNTGC 4 cut(s) 752, 786, 3442, 3476
BciT130I CCWGG 2 cut(s) 591, 1388
BciVI GTATCC 1 cut(s) 3163
BclI TGATCA 2 cut(s) 301, 3307
BcoDI GTCTC 4 cut(s) 1548, 2666, 2721, 2841
BcuI ACTAGT 1 cut(s) 1502
BfaI CTAG 5 cut(s) 833, 1340, 1487, 1503, 1979
BfmI CTRYAG 5 cut(s) 280, 771, 1895, 2123, 2221
BfoI RGCGCY 1 cut(s) 1063
BfrI CTTAAG 2 cut(s) 329, 731
BfuAI ACCTGC 1 cut(s) 1789
BfuI GTATCC 1 cut(s) 3163
BglII AGATCT 1 cut(s) 1036
BisI GCNGC 5 cut(s) 33, 45, 1115, 2865, 3733
BlsI GCNGC 5 cut(s) 34, 46, 1116, 2866, 3734
BmcAI AGTACT 2 cut(s) 1686, 3614
Bme1390I CCNGG 2 cut(s) 591, 1388
Bme18I GGWCC 3 cut(s) 587, 829, 3159
BmeRI GACNNNNNGTC 1 cut(s) 1354
BmeT110I CYCGRG 1 cut(s) 3064
BmgT120I GGNCC 4 cut(s) 587, 829, 1306, 3159
BmiI GGNNCC 5 cut(s) 325, 747, 1512, 2382, 2438
BmrFI CCNGG 2 cut(s) 591, 1388
BmrI ACTGGG 1 cut(s) 1435
BmsI GCATC 6 cut(s) 511, 561, 964, 965, 1010, 3019
BmtI GCTAGC 1 cut(s) 1490
BmuI ACTGGG 1 cut(s) 1435
BoxI GACNNNNGTC 1 cut(s) 1244
BpiI GAAGAC 3 cut(s) 1290, 1353, 1806
BpmI CTGGAG 1 cut(s) 612
Bpu10I CCTNAGC 3 cut(s) 1674, 2138, 3504
Bpu14I TTCGAA 1 cut(s) 250
BpuEI CTTGAG 6 cut(s) 98, 539, 2152, 2315, 3399, 3637
BsaAI YACGTR 1 cut(s) 3237
BsaBI GATNNNNATC 2 cut(s) 856, 3329
BsaI GGTCTC 1 cut(s) 2666
BsaJI CCNNGG 7 cut(s) 384, 1005, 1267, 1386, 1387, 3063, 3447
BsaWI WCCGGW 1 cut(s) 3623
BsaXI ACNNNNNCTCC 2 cut(s) 3605, 3635
Bse118I RCCGGY 1 cut(s) 3006
Bse1I ACTGG 4 cut(s) 585, 1430, 2654, 3478
Bse21I CCTNAGG 1 cut(s) 3251
Bse3DI GCAATG 3 cut(s) 984, 1359, 2011
Bse8I GATNNNNATC 2 cut(s) 856, 3329
BseAI TCCGGA 1 cut(s) 3623
BseBI CCWGG 2 cut(s) 591, 1388
BseDI CCNNGG 7 cut(s) 384, 1005, 1267, 1386, 1387, 3063, 3447
BseJI GATNNNNATC 2 cut(s) 856, 3329
BseMI GCAATG 3 cut(s) 984, 1359, 2011
BseMII CTCAG 9 cut(s) 1170, 1665, 2074, 2129, 2413, 2737, 2943, 3164, 3495
BseNI ACTGG 4 cut(s) 585, 1430, 2654, 3478
BseRI GAGGAG 2 cut(s) 245, 486
BseSI GKGCMC 1 cut(s) 3071
BseXI GCAGC 5 cut(s) 19, 56, 1126, 2851, 3719
BsgI GTGCAG 3 cut(s) 377, 495, 2281
Bsh1236I CGCG 2 cut(s) 878, 3362
BshFI GGCC 6 cut(s) 1308, 1929, 2423, 2573, 2708, 3502
BshNI GGYRCC 2 cut(s) 745, 2436
BsiHKAI GWGCWC 3 cut(s) 246, 1022, 3623
BsiHKCI CYCGRG 1 cut(s) 3064
BsiSI CCGG 2 cut(s) 3007, 3624
BslFI GGGAC 3 cut(s) 3121, 3269, 3496
BsmAI GTCTC 4 cut(s) 1548, 2666, 2721, 2841
BsmBI CGTCTC 1 cut(s) 2721
BsmFI GGGAC 3 cut(s) 3121, 3269, 3496
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 6 cut(s) 1308, 1929, 2423, 2573, 2708, 3502
Bso31I GGTCTC 1 cut(s) 2666
BsoBI CYCGRG 1 cut(s) 3064
Bsp119I TTCGAA 1 cut(s) 250
Bsp1286I GDGCHC 4 cut(s) 246, 1022, 3071, 3623
Bsp13I TCCGGA 1 cut(s) 3623
Bsp1407I TGTACA 2 cut(s) 1879, 3643
Bsp19I CCATGG 1 cut(s) 3447
Bsp68I TCGCGA 1 cut(s) 878
BspANI GGCC 6 cut(s) 1308, 1929, 2423, 2573, 2708, 3502
BspCNI CTCAG 9 cut(s) 1171, 1666, 2073, 2130, 2412, 2736, 2944, 3163, 3496
BspEI TCCGGA 1 cut(s) 3623
BspFNI CGCG 2 cut(s) 878, 3362
BspLI GGNNCC 5 cut(s) 325, 747, 1512, 2382, 2438
BspMAI CTGCAG 2 cut(s) 775, 1899
BspMI ACCTGC 1 cut(s) 1789
BspOI GCTAGC 1 cut(s) 1490
BspPI GGATC 5 cut(s) 148, 318, 331, 859, 2993
BspT104I TTCGAA 1 cut(s) 250
BspT107I GGYRCC 2 cut(s) 745, 2436
BspTI CTTAAG 2 cut(s) 329, 731
BspTNI GGTCTC 1 cut(s) 2666
BsrBI CCGCTC 1 cut(s) 1846
BsrDI GCAATG 3 cut(s) 984, 1359, 2011
BsrFI RCCGGY 1 cut(s) 3006
BsrGI TGTACA 2 cut(s) 1879, 3643
BsrI ACTGG 4 cut(s) 585, 1430, 2654, 3478
BssAI RCCGGY 1 cut(s) 3006
BssECI CCNNGG 7 cut(s) 384, 1005, 1267, 1386, 1387, 3063, 3447
BssNAI GTATAC 2 cut(s) 2150, 3391
BssT1I CCWWGG 4 cut(s) 384, 1005, 1267, 3447
Bst1107I GTATAC 2 cut(s) 2150, 3391
Bst2UI CCWGG 2 cut(s) 591, 1388
Bst6I CTCTTC 2 cut(s) 1883, 2627
BstAFI CTTAAG 2 cut(s) 329, 731
BstAPI GCANNNNNTGC 2 cut(s) 1657, 2308
BstAUI TGTACA 2 cut(s) 1879, 3643
BstBAI YACGTR 1 cut(s) 3237
BstBI TTCGAA 1 cut(s) 250
BstC8I GCNNGC 6 cut(s) 782, 1112, 1306, 1488, 1606, 3004
BstDSI CCRYGG 1 cut(s) 3447
BstFNI CGCG 2 cut(s) 878, 3362
BstH2I RGCGCY 1 cut(s) 1063
BstHHI GCGC 2 cut(s) 1062, 3362
BstMAI GTCTC 4 cut(s) 1548, 2666, 2721, 2841
BstMWI GCNNNNNNNGC 5 cut(s) 41, 1483, 1657, 2308, 3359
BstNI CCWGG 2 cut(s) 591, 1388
BstNSI RCATGY 3 cut(s) 1357, 1660, 3092
BstPAI GACNNNNGTC 1 cut(s) 1244
BstSCI CCNGG 2 cut(s) 589, 1386
BstSFI CTRYAG 5 cut(s) 280, 771, 1895, 2123, 2221
BstSLI GKGCMC 1 cut(s) 3071
BstUI CGCG 2 cut(s) 878, 3362
BstV1I GCAGC 5 cut(s) 19, 56, 1126, 2851, 3719
BstV2I GAAGAC 3 cut(s) 1290, 1353, 1806
BstX2I RGATCY 2 cut(s) 323, 1036
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstYI RGATCY 2 cut(s) 323, 1036
BstZ17I GTATAC 2 cut(s) 2150, 3391
Bsu36I CCTNAGG 1 cut(s) 3251
BsuI GTATCC 1 cut(s) 3163
BsuRI GGCC 6 cut(s) 1308, 1929, 2423, 2573, 2708, 3502
BtgI CCRYGG 1 cut(s) 3447
BtgZI GCGATG 2 cut(s) 3214, 3450
BtsI GCAGTG 2 cut(s) 483, 2294
BtsIMutI CAGTG 6 cut(s) 297, 483, 959, 2294, 3158, 3580
BtuMI TCGCGA 1 cut(s) 878
BveI ACCTGC 1 cut(s) 1789
Cac8I GCNNGC 6 cut(s) 782, 1112, 1306, 1488, 1606, 3004
CaiI CAGNNNCTG 2 cut(s) 482, 1393
CfoI GCGC 2 cut(s) 1062, 3362
Cfr10I RCCGGY 1 cut(s) 3006
Cfr13I GGNCC 4 cut(s) 587, 829, 1306, 3159
Csp6I GTAC 8 cut(s) 443, 578, 1685, 1880, 3316, 3539, 3613, 3644
CviQI GTAC 8 cut(s) 443, 578, 1685, 1880, 3316, 3539, 3613, 3644
DraI TTTAAA 1 cut(s) 103
DriI GACNNNNNGTC 1 cut(s) 1354
EaeI YGGCCR 3 cut(s) 1927, 2421, 2571
Eam1104I CTCTTC 2 cut(s) 1883, 2627
Eam1105I GACNNNNNGTC 1 cut(s) 1354
EarI CTCTTC 2 cut(s) 1883, 2627
Ecl136II GAGCTC 3 cut(s) 244, 1020, 3621
Eco130I CCWWGG 4 cut(s) 384, 1005, 1267, 3447
Eco147I AGGCCT 1 cut(s) 3502
Eco24I GRGCYC 3 cut(s) 246, 1022, 3623
Eco31I GGTCTC 1 cut(s) 2666
Eco32I GATATC 1 cut(s) 1905
Eco47I GGWCC 3 cut(s) 587, 829, 3159
Eco47III AGCGCT 1 cut(s) 1061
Eco53kI GAGCTC 3 cut(s) 244, 1020, 3621
Eco57I CTGAAG 5 cut(s) 1302, 1429, 1766, 2262, 3116
Eco81I CCTNAGG 1 cut(s) 3251
Eco88I CYCGRG 1 cut(s) 3064
EcoICRI GAGCTC 3 cut(s) 244, 1020, 3621
EcoRII CCWGG 2 cut(s) 589, 1386
EcoRV GATATC 1 cut(s) 1905
EcoT14I CCWWGG 4 cut(s) 384, 1005, 1267, 3447
EcoT22I ATGCAT 1 cut(s) 1969
EcoT38I GRGCYC 3 cut(s) 246, 1022, 3623
ErhI CCWWGG 4 cut(s) 384, 1005, 1267, 3447
Esp3I CGTCTC 1 cut(s) 2721
FalI AAGNNNNNCTT 4 cut(s) 368, 400, 1147, 1179
FaqI GGGAC 3 cut(s) 3121, 3269, 3496
FauI CCCGC 3 cut(s) 1103, 1297, 3270
FauNDI CATATG 2 cut(s) 1547, 1963
FbaI TGATCA 2 cut(s) 301, 3307
FblI GTMKAC 2 cut(s) 2149, 3390
Fnu4HI GCNGC 5 cut(s) 33, 45, 1115, 2865, 3733
FriOI GRGCYC 3 cut(s) 246, 1022, 3623
Fsp4HI GCNGC 5 cut(s) 33, 45, 1115, 2865, 3733
FspBI CTAG 5 cut(s) 833, 1340, 1487, 1503, 1979
GlaI GCGC 2 cut(s) 1061, 3361
GluI GCNGC 5 cut(s) 33, 45, 1115, 2865, 3733
GsuI CTGGAG 1 cut(s) 612
HaeII RGCGCY 1 cut(s) 1063
HaeIII GGCC 6 cut(s) 1308, 1929, 2423, 2573, 2708, 3502
HapII CCGG 2 cut(s) 3007, 3624
HhaI GCGC 2 cut(s) 1062, 3362
Hin6I GCGC 2 cut(s) 1060, 3360
HinP1I GCGC 2 cut(s) 1060, 3360
HincII GTYRAC 2 cut(s) 728, 3384
HindII GTYRAC 2 cut(s) 728, 3384
HindIII AAGCTT 1 cut(s) 1932
HpaII CCGG 2 cut(s) 3007, 3624
HphI GGTGA 4 cut(s) 919, 1453, 2570, 2653
Hpy166II GTNNAC 8 cut(s) 728, 1575, 2150, 2497, 2736, 2773, 3384, 3391
Hpy8I GTNNAC 8 cut(s) 728, 1575, 2150, 2497, 2736, 2773, 3384, 3391
Hpy99I CGWCG 1 cut(s) 505
HpyCH4IV ACGT 1 cut(s) 3236
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 1483, 1657, 2308, 3359
HpySE526I ACGT 1 cut(s) 3236
HspAI GCGC 2 cut(s) 1060, 3360
Kpn2I TCCGGA 1 cut(s) 3623
Ksp22I TGATCA 2 cut(s) 301, 3307
LmnI GCTCC 6 cut(s) 41, 529, 1017, 1057, 1952, 3626
Lsp1109I GCAGC 5 cut(s) 19, 56, 1126, 2851, 3719
LweI GCATC 6 cut(s) 511, 561, 964, 965, 1010, 3019
MaeI CTAG 5 cut(s) 833, 1340, 1487, 1503, 1979
MaeII ACGT 1 cut(s) 3236
MbiI CCGCTC 1 cut(s) 1846
MfeI CAATTG 2 cut(s) 1883, 1942
MflI RGATCY 2 cut(s) 323, 1036
MhlI GDGCHC 4 cut(s) 246, 1022, 3071, 3623
MlsI TGGCCA 3 cut(s) 1929, 2423, 2573
MluNI TGGCCA 3 cut(s) 1929, 2423, 2573
MlyI GAGTC 6 cut(s) 457, 976, 1868, 1900, 2608, 3077
Mox20I TGGCCA 3 cut(s) 1929, 2423, 2573
Mph1103I ATGCAT 1 cut(s) 1969
MroI TCCGGA 1 cut(s) 3623
MroXI GAANNNNTTC 4 cut(s) 792, 807, 861, 1038
MscI TGGCCA 3 cut(s) 1929, 2423, 2573
MslI CAYNNNNRTG 5 cut(s) 972, 1088, 2417, 2624, 3093
Msp20I TGGCCA 3 cut(s) 1929, 2423, 2573
MspA1I CMGCKG 1 cut(s) 151
MspCI CTTAAG 2 cut(s) 329, 731
MspI CCGG 2 cut(s) 3007, 3624
MspR9I CCNGG 2 cut(s) 591, 1388
MssI GTTTAAAC 1 cut(s) 103
MunI CAATTG 2 cut(s) 1883, 1942
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 2 cut(s) 591, 1388
MvnI CGCG 2 cut(s) 878, 3362
MwoI GCNNNNNNNGC 5 cut(s) 41, 1483, 1657, 2308, 3359
NcoI CCATGG 1 cut(s) 3447
NdeI CATATG 2 cut(s) 1547, 1963
NheI GCTAGC 1 cut(s) 1486
NlaIV GGNNCC 5 cut(s) 325, 747, 1512, 2382, 2438
NmeAIII GCCGAG 1 cut(s) 2734
NmuCI GTSAC 7 cut(s) 907, 1101, 1459, 1759, 2467, 2617, 3080
NruI TCGCGA 1 cut(s) 878
NsiI ATGCAT 1 cut(s) 1969
NspI RCATGY 3 cut(s) 1357, 1660, 3092
NspV TTCGAA 1 cut(s) 250
OliI CACNNNNGTG 1 cut(s) 2624
PasI CCCWGGG 1 cut(s) 1387
PceI AGGCCT 1 cut(s) 3502
PctI GAATGC 1 cut(s) 47
PdmI GAANNNNTTC 4 cut(s) 792, 807, 861, 1038
PfeI GAWTC 8 cut(s) 137, 209, 857, 1175, 1293, 1833, 2510, 3242
PflMI CCANNNNNTGG 3 cut(s) 935, 1393, 1572
PfoI TCCNGGA 1 cut(s) 589
PkrI GCNGC 5 cut(s) 34, 46, 1116, 2866, 3734
PleI GAGTC 6 cut(s) 456, 976, 1868, 1899, 2607, 3077
PmeI GTTTAAAC 1 cut(s) 103
PpsI GAGTC 6 cut(s) 456, 976, 1868, 1899, 2607, 3077
Ppu21I YACGTR 1 cut(s) 3237
PshAI GACNNNNGTC 1 cut(s) 1244
PshBI ATTAAT 1 cut(s) 1869
Psp124BI GAGCTC 3 cut(s) 246, 1022, 3623
Psp6I CCWGG 2 cut(s) 589, 1386
PspGI CCWGG 2 cut(s) 589, 1386
PspN4I GGNNCC 5 cut(s) 325, 747, 1512, 2382, 2438
PspPI GGNCC 4 cut(s) 587, 829, 1306, 3159
PsrI GAACNNNNNNTAC 4 cut(s) 1982, 2014, 2373, 2405
PstI CTGCAG 2 cut(s) 775, 1899
PstNI CAGNNNCTG 2 cut(s) 482, 1393
PsuI RGATCY 2 cut(s) 323, 1036
RruI TCGCGA 1 cut(s) 878
RsaI GTAC 8 cut(s) 444, 579, 1686, 1881, 3317, 3540, 3614, 3645
RsaNI GTAC 8 cut(s) 443, 578, 1685, 1880, 3316, 3539, 3613, 3644
RseI CAYNNNNRTG 5 cut(s) 972, 1088, 2417, 2624, 3093
SacI GAGCTC 3 cut(s) 246, 1022, 3623
SatI GCNGC 5 cut(s) 33, 45, 1115, 2865, 3733
Sau96I GGNCC 4 cut(s) 587, 829, 1306, 3159
ScaI AGTACT 2 cut(s) 1686, 3614
SchI GAGTC 6 cut(s) 457, 976, 1868, 1900, 2608, 3077
ScrFI CCNGG 2 cut(s) 591, 1388
SduI GDGCHC 4 cut(s) 246, 1022, 3071, 3623
SfaNI GCATC 6 cut(s) 511, 561, 964, 965, 1010, 3019
SfcI CTRYAG 5 cut(s) 280, 771, 1895, 2123, 2221
SfuI TTCGAA 1 cut(s) 250
SinI GGWCC 3 cut(s) 587, 829, 3159
SmiMI CAYNNNNRTG 5 cut(s) 972, 1088, 2417, 2624, 3093
SmlI CTYRAG 8 cut(s) 113, 329, 554, 731, 2131, 2330, 3414, 3616
SmoI CTYRAG 8 cut(s) 113, 329, 554, 731, 2131, 2330, 3414, 3616
SpeI ACTAGT 1 cut(s) 1502
SseBI AGGCCT 1 cut(s) 3502
SspMI CTAG 5 cut(s) 833, 1340, 1487, 1503, 1979
SstI GAGCTC 3 cut(s) 246, 1022, 3623
StuI AGGCCT 1 cut(s) 3502
StyD4I CCNGG 2 cut(s) 589, 1386
StyI CCWWGG 4 cut(s) 384, 1005, 1267, 3447
TaiI ACGT 1 cut(s) 3239
TaqI TCGA 5 cut(s) 250, 569, 715, 2174, 3289
TatI WGTACW 5 cut(s) 577, 1684, 1879, 3612, 3643
TfiI GAWTC 8 cut(s) 137, 209, 857, 1175, 1293, 1833, 2510, 3242
TscAI CASTG 6 cut(s) 304, 483, 966, 2294, 3158, 3580
TseFI GTSAC 7 cut(s) 907, 1101, 1459, 1759, 2467, 2617, 3080
TseI GCWGC 5 cut(s) 32, 44, 1114, 2864, 3732
Tsp45I GTSAC 7 cut(s) 907, 1101, 1459, 1759, 2467, 2617, 3080
TspGWI ACGGA 4 cut(s) 2774, 2799, 2870, 3516
TspRI CASTG 6 cut(s) 304, 483, 966, 2294, 3158, 3580
Van91I CCANNNNNTGG 3 cut(s) 935, 1393, 1572
Vha464I CTTAAG 2 cut(s) 329, 731
VpaK11BI GGWCC 3 cut(s) 587, 829, 3159
VspI ATTAAT 1 cut(s) 1869
XapI RAATTY 3 cut(s) 547, 1721, 2170
XceI RCATGY 3 cut(s) 1357, 1660, 3092
XcmI CCANNNNNNNNNTGG 1 cut(s) 2322
XmiI GTMKAC 2 cut(s) 2149, 3390
XmnI GAANNNNTTC 4 cut(s) 792, 807, 861, 1038
XspI CTAG 5 cut(s) 833, 1340, 1487, 1503, 1979
ZrmI AGTACT 2 cut(s) 1686, 3614
Zsp2I ATGCAT 1 cut(s) 1969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.