Rh5DG277500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005190
Physical Location & Seq
Forward (+)
33967728 .. 33973643
5916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG277500.1

Sequence Viewer

Length: 1911 bp
ATGAGAGAGAAAGAGGCGGCCAAGGAGGAGGAAAGAGGTCGGAGCCAGAGAAGGCGAAAGAGGTCGTCGGTTACTGGACCGGGGAAAGAGGACGATCGGTCGCGGTGTGTGCGGTGTTTGAGGACGTTCGGTCGAGCTGATCCAGACAATTTTACTCTTGAAATTAGACATGGGGGTGTATTTTCTAATGGAGAGTATGTGGGTGGTTCTGTATGTTGGGCAGACAACTATGATGTTAACTTATTTAGCAAGTGGGACTTGGCAGTAATTGCTAGAAATCTTGGATATGATGTTGAGAGAATTCAATACTTTTTCTACCATAAAACTGATGGTTTGGTAGAGATAGATAGTGACATAAGAGTTCTAAACCAATCCACTAGAAAAGTTTCTGTTGCCTATATTAGTGATTGCATAGTTTTGGATGAAACTCTTGCATTTAATGAAGAATCACAAGTTACTCATCATGTTCAAGCTGACAATGAAGCGGGTGCATATGGGGATGATAAAGCGGATTATGATATAGGACTTGATGTTAATGTCTTGGCAGATAATGATTTGGACGGAGATTCCAGCAATGATGAAGATGTACTAGATCCTGAAGATGCACAGTGGTTTGTGGATGAAGAATATGTTCAGAGCGATGAAGGTATGAGGCAGTTCAAGAGTTATGTTGTAGATGAAATTGAAGTTGATCATCATGTGGAACCTGGAGAGCAGTCTGATGACTGGGCTATGGACAGCGATGATCTTGAGAGCCTTGATGAGAGTTCAAGTGATGAGGAAGTTAATGAGCCTGTTAAGTACATGAAGCCTAAGAAAAAGCAACCAAAGTTCAAGCAGTATAGAAGAGATACTGACTTACGCGATCCATACTTCAAACTGGGTTTGGAGTTTCCAACTATGGCACAATGTAGGGAGGCCATAAGAGAATTGTCTATCAAATCTGGAAGGCCACTAAGTTTTGACGAAAATGATTCTGACAAGGTTATATTGAAATGTAAAGGACAATGTAAGGGAAGTTGTCCATTCAAAATATATGCATCTATGGTTGGGAATGGTCCTACTGTGAGGGTCAAGACTTATGAGCCATTGCATCACTGCGGGAGGTCAAAAAAAATAAAGCATGCTACTTCTACTTGGATAGCAGACAGGTTTGATGAGGTCTTCAGATCCAATCCCAAAATAAAACCTAGAGACTTTATTCGAATGGTGGCTAAGCAGTACGGGATTGATATTACACTTGATCAGTTTTATAAGGCCAAGGAAAAAGCCAAGAGGAGGATACAAGGTGGCATTCAGGAGCAATATGCGAGAGGACAAGCACTAGAACCTCCTCCTCATGTTCCAGCGAGCGACAATATGTACAAGGCAAGTGGAACAGCAAGAGGAAGGGCTAGAGGAACCGGCACAAATAGAGGAAGAGGTATTGGAAATTCAATTATGACGGAAGAAGCACAATTTGGACAAGGAACAAGAGGACAAGGAAGACATGGTGTTCAATATGTTAGAGTTGGGGGGACTAGAGAAGGAAGATGCCAATTTGTTGCTCTAACAAGAGAAAGAGGACAGTTTGAAAGCTTTATTGGAAGGTCACAATCTACAAGTTTCGGTACTAGAGGAGGAGGACAAACATTTGCAAGTGCCGGAAGAGGAAGAAGCCAATTCATTGCTCCAACAAGAGGAAAAGGGCAACCTACAAGCTTTGGTAATGGTGGAGGACAAGCTCTCCCAGGTATTGAAAGAGGAAGGGGACAATATGTCAATCGTGCCACTCGTATAACTCAAGTGATAGTGCATTCTACAATCAGTACTGAAGCTACCACATCTCAACCAATTTCTAAAGCTAGAAGTAGTGAATCAAGTACAAGGCATGTTACTCCTGCCAACAGTAACACACATTGGAAGCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

636

Amino Acids

71.37

Weight (kDa)

6.94

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 49 - 122 2.7e-08 PB1-like domain
DBD_Tnp_Mut PF03108 293 - 350 6.2e-10 MuDR family transposase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1254
AccII CGCG 2 cut(s) 103, 864
AciI CCGC 6 cut(s) 17, 103, 112, 485, 509, 1101
AclWI GGATC 4 cut(s) 134, 587, 860, 1164
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 2 cut(s) 300, 1432
AcuI CTGAAG 3 cut(s) 618, 1150, 1833
AfaI GTAC 7 cut(s) 588, 803, 1223, 1364, 1612, 1810, 1864
AfiI CCNNNNNNNGG 2 cut(s) 1278, 1679
AhdI GACNNNNNGTC 3 cut(s) 97, 129, 1757
AjnI CCWGG 2 cut(s) 706, 1729
AjuI GAANNNNNNNTTGG 4 cut(s) 1443, 1475, 1566, 1598
AluBI AGCT 8 cut(s) 137, 473, 1578, 1701, 1724, 1817, 1844, 1906
AluI AGCT 8 cut(s) 137, 473, 1578, 1701, 1724, 1817, 1844, 1906
Alw26I GTCTC 1 cut(s) 1188
AlwI GGATC 4 cut(s) 134, 587, 860, 1164
AoxI GGCC 4 cut(s) 18, 918, 950, 1257
ApoI RAATTY 2 cut(s) 300, 1432
Asp700I GAANNNNTTC 2 cut(s) 385, 630
AspS9I GGNCC 2 cut(s) 77, 1058
AsuC2I CCSGG 1 cut(s) 81
AsuII TTCGAA 1 cut(s) 1204
AvaII GGWCC 2 cut(s) 77, 1058
BbsI GAAGAC 2 cut(s) 1156, 1492
BccI CCATC 1 cut(s) 323
BciT130I CCWGG 2 cut(s) 708, 1731
BciVI GTATCC 1 cut(s) 1275
BclI TGATCA 2 cut(s) 691, 1243
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 1188
BfaI CTAG 9 cut(s) 273, 378, 590, 1191, 1325, 1395, 1521, 1614, 1845
BfmI CTRYAG 1 cut(s) 1907
BfuI GTATCC 1 cut(s) 1275
BisI GCNGC 1 cut(s) 18
BlpI GCTNAGC 1 cut(s) 1215
BlsI GCNGC 1 cut(s) 19
BmcAI AGTACT 1 cut(s) 1810
Bme1390I CCNGG 3 cut(s) 81, 708, 1731
Bme18I GGWCC 2 cut(s) 77, 1058
BmeRI GACNNNNNGTC 3 cut(s) 97, 129, 1757
BmgT120I GGNCC 2 cut(s) 77, 1058
BmiI GGNNCC 3 cut(s) 44, 705, 1402
BmrFI CCNGG 3 cut(s) 81, 708, 1731
BmrI ACTGGG 2 cut(s) 736, 890
BmsI GCATC 4 cut(s) 592, 1049, 1102, 1523
BmuI ACTGGG 2 cut(s) 736, 890
BpiI GAAGAC 2 cut(s) 1156, 1492
BpmI CTGGAG 1 cut(s) 729
Bpu1102I GCTNAGC 1 cut(s) 1215
Bpu14I TTCGAA 1 cut(s) 1204
BpuEI CTTGAG 2 cut(s) 770, 1767
BpuMI CCSGG 1 cut(s) 81
BsaJI CCNNGG 4 cut(s) 21, 80, 1260, 1729
BsaXI ACNNNNNCTCC 4 cut(s) 183, 213, 1710, 1740
Bsc4I CCNNNNNNNGG 2 cut(s) 1278, 1679
Bse118I RCCGGY 1 cut(s) 1403
Bse1I ACTGG 3 cut(s) 79, 731, 885
Bse3DI GCAATG 3 cut(s) 580, 1088, 1665
BseBI CCWGG 2 cut(s) 708, 1731
BseDI CCNNGG 4 cut(s) 21, 80, 1260, 1729
BseGI GGATG 3 cut(s) 427, 505, 625
BseLI CCNNNNNNNGG 2 cut(s) 1278, 1679
BseMI GCAATG 3 cut(s) 580, 1088, 1665
BseNI ACTGG 3 cut(s) 79, 731, 885
BseRI GAGGAG 6 cut(s) 41, 1291, 1323, 1326, 1632, 1635
Bsh1236I CGCG 2 cut(s) 103, 864
Bsh1285I CGRYCG 3 cut(s) 97, 101, 133
BshFI GGCC 4 cut(s) 20, 920, 952, 1259
BsiEI CGRYCG 3 cut(s) 97, 101, 133
BsiSI CCGG 3 cut(s) 80, 1404, 1644
BslFI GGGAC 3 cut(s) 269, 1531, 1764
BslI CCNNNNNNNGG 2 cut(s) 1278, 1679
BsmAI GTCTC 1 cut(s) 1188
BsmFI GGGAC 3 cut(s) 269, 1531, 1764
BsmI GAATGC 2 cut(s) 1293, 1795
BsnI GGCC 4 cut(s) 20, 920, 952, 1259
Bsp119I TTCGAA 1 cut(s) 1204
Bsp1407I TGTACA 1 cut(s) 1362
Bsp143I GATC 8 cut(s) 94, 139, 592, 691, 745, 865, 1169, 1243
Bsp1720I GCTNAGC 1 cut(s) 1215
BspACI CCGC 6 cut(s) 17, 103, 112, 485, 509, 1101
BspANI GGCC 4 cut(s) 20, 920, 952, 1259
BspFNI CGCG 2 cut(s) 103, 864
BspLI GGNNCC 3 cut(s) 44, 705, 1402
BspPI GGATC 4 cut(s) 134, 587, 860, 1164
BspT104I TTCGAA 1 cut(s) 1204
BsrDI GCAATG 3 cut(s) 580, 1088, 1665
BsrFI RCCGGY 1 cut(s) 1403
BsrGI TGTACA 1 cut(s) 1362
BsrI ACTGG 3 cut(s) 79, 731, 885
BssAI RCCGGY 1 cut(s) 1403
BssECI CCNNGG 4 cut(s) 21, 80, 1260, 1729
BssMI GATC 8 cut(s) 94, 139, 592, 691, 745, 865, 1169, 1243
BssT1I CCWWGG 2 cut(s) 21, 1260
Bst2UI CCWGG 2 cut(s) 708, 1731
Bst4CI ACNGT 4 cut(s) 609, 1066, 1569, 1889
Bst6I CTCTTC 3 cut(s) 841, 1414, 1642
BstAPI GCANNNNNTGC 1 cut(s) 269
BstAUI TGTACA 1 cut(s) 1362
BstBI TTCGAA 1 cut(s) 1204
BstC8I GCNNGC 2 cut(s) 1125, 1351
BstDEI CTNAG 3 cut(s) 813, 956, 1215
BstF5I GGATG 3 cut(s) 427, 505, 625
BstFNI CGCG 2 cut(s) 103, 864
BstKTI GATC 8 cut(s) 97, 142, 595, 694, 748, 868, 1172, 1246
BstMAI GTCTC 1 cut(s) 1188
BstMBI GATC 8 cut(s) 94, 139, 592, 691, 745, 865, 1169, 1243
BstMCI CGRYCG 3 cut(s) 97, 101, 133
BstMWI GCNNNNNNNGC 2 cut(s) 109, 269
BstNI CCWGG 2 cut(s) 708, 1731
BstNSI RCATGY 2 cut(s) 1127, 1874
BstSCI CCNGG 3 cut(s) 79, 706, 1729
BstSFI CTRYAG 1 cut(s) 1907
BstUI CGCG 2 cut(s) 103, 864
BstV2I GAAGAC 2 cut(s) 1156, 1492
BstX2I RGATCY 2 cut(s) 592, 1169
BstYI RGATCY 2 cut(s) 592, 1169
BsuI GTATCC 1 cut(s) 1275
BsuRI GGCC 4 cut(s) 20, 920, 952, 1259
BtgZI GCGATG 2 cut(s) 654, 756
BtsCI GGATG 3 cut(s) 427, 505, 625
BtsI GCAGTG 1 cut(s) 1096
BtsIMutI CAGTG 2 cut(s) 614, 1096
Cac8I GCNNGC 2 cut(s) 1125, 1351
Cfr10I RCCGGY 1 cut(s) 1403
Cfr13I GGNCC 2 cut(s) 77, 1058
Csp6I GTAC 7 cut(s) 587, 802, 1222, 1363, 1611, 1809, 1863
CspCI CAANNNNNGTGG 2 cut(s) 1354, 1389
CviAII CATG 8 cut(s) 170, 464, 698, 805, 1124, 1340, 1490, 1871
CviQI GTAC 7 cut(s) 587, 802, 1222, 1363, 1611, 1809, 1863
DdeI CTNAG 3 cut(s) 813, 956, 1215
DpnI GATC 8 cut(s) 96, 141, 594, 693, 747, 867, 1171, 1245
DpnII GATC 8 cut(s) 94, 139, 592, 691, 745, 865, 1169, 1243
DriI GACNNNNNGTC 3 cut(s) 97, 129, 1757
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 3 cut(s) 841, 1414, 1642
Eam1105I GACNNNNNGTC 3 cut(s) 97, 129, 1757
EarI CTCTTC 3 cut(s) 841, 1414, 1642
Eco130I CCWWGG 2 cut(s) 21, 1260
Eco47I GGWCC 2 cut(s) 77, 1058
Eco57I CTGAAG 3 cut(s) 618, 1150, 1833
EcoRI GAATTC 1 cut(s) 300
EcoRII CCWGG 2 cut(s) 706, 1729
EcoT14I CCWWGG 2 cut(s) 21, 1260
EcoT22I ATGCAT 1 cut(s) 1042
ErhI CCWWGG 2 cut(s) 21, 1260
FaeI CATG 8 cut(s) 173, 467, 701, 808, 1127, 1343, 1493, 1874
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FaqI GGGAC 3 cut(s) 269, 1531, 1764
FatI CATG 8 cut(s) 169, 463, 697, 804, 1123, 1339, 1489, 1870
FauI CCCGC 2 cut(s) 478, 1094
FauNDI CATATG 1 cut(s) 493
FbaI TGATCA 2 cut(s) 691, 1243
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 3 cut(s) 434, 512, 632
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 9 cut(s) 273, 378, 590, 1191, 1325, 1395, 1521, 1614, 1845
GluI GCNGC 1 cut(s) 18
GsuI CTGGAG 1 cut(s) 729
HaeIII GGCC 4 cut(s) 20, 920, 952, 1259
HapII CCGG 3 cut(s) 80, 1404, 1644
Hin1II CATG 8 cut(s) 173, 467, 701, 808, 1127, 1343, 1493, 1874
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HindIII AAGCTT 2 cut(s) 1576, 1699
HinfI GANTC 4 cut(s) 446, 566, 974, 1856
HpaI GTTAAC 1 cut(s) 238
HpaII CCGG 3 cut(s) 80, 1404, 1644
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 5 cut(s) 42, 636, 721, 979, 1169
Hpy188III TCNNGA 8 cut(s) 143, 158, 596, 661, 749, 945, 1075, 1298
Hpy8I GTNNAC 1 cut(s) 238
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 7 cut(s) 45, 638, 942, 1383, 1520, 1581, 1740
HpyCH4III ACNGT 4 cut(s) 609, 1066, 1569, 1889
HpyCH4IV ACGT 1 cut(s) 125
HpyCH4V TGCA 8 cut(s) 411, 434, 491, 605, 1040, 1093, 1637, 1795
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 269
HpyF3I CTNAG 3 cut(s) 813, 956, 1215
HpySE526I ACGT 1 cut(s) 125
Hsp92II CATG 8 cut(s) 173, 467, 701, 808, 1127, 1343, 1493, 1874
Ksp22I TGATCA 2 cut(s) 691, 1243
KspAI GTTAAC 1 cut(s) 238
Kzo9I GATC 8 cut(s) 94, 139, 592, 691, 745, 865, 1169, 1243
LmnI GCTCC 3 cut(s) 42, 1300, 1675
LweI GCATC 4 cut(s) 592, 1049, 1102, 1523
MaeI CTAG 9 cut(s) 273, 378, 590, 1191, 1325, 1395, 1521, 1614, 1845
MaeII ACGT 1 cut(s) 125
MaeIII GTNAC 6 cut(s) 70, 350, 454, 1590, 1873, 1889
MalI GATC 8 cut(s) 96, 141, 594, 693, 747, 867, 1171, 1245
MboI GATC 8 cut(s) 94, 139, 592, 691, 745, 865, 1169, 1243
MflI RGATCY 2 cut(s) 592, 1169
MmeI TCCRAC 3 cut(s) 20, 920, 1697
Mph1103I ATGCAT 1 cut(s) 1042
MroXI GAANNNNTTC 2 cut(s) 385, 630
MseI TTAA 5 cut(s) 237, 438, 534, 786, 798
MslI CAYNNNNRTG 1 cut(s) 174
MspI CCGG 3 cut(s) 80, 1404, 1644
MspR9I CCNGG 3 cut(s) 81, 708, 1731
Mva1269I GAATGC 2 cut(s) 1293, 1795
MvaI CCWGG 2 cut(s) 708, 1731
MvnI CGCG 2 cut(s) 103, 864
MwoI GCNNNNNNNGC 2 cut(s) 109, 269
NciI CCSGG 1 cut(s) 81
NdeI CATATG 1 cut(s) 493
NdeII GATC 8 cut(s) 94, 139, 592, 691, 745, 865, 1169, 1243
NlaIII CATG 8 cut(s) 173, 467, 701, 808, 1127, 1343, 1493, 1874
NlaIV GGNNCC 3 cut(s) 44, 705, 1402
NmuCI GTSAC 2 cut(s) 350, 1590
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 2 cut(s) 1127, 1874
NspV TTCGAA 1 cut(s) 1204
PaeI GCATGC 1 cut(s) 1127
PcsI WCGNNNNNNNCGW 1 cut(s) 1771
PctI GAATGC 2 cut(s) 1293, 1795
PdmI GAANNNNTTC 2 cut(s) 385, 630
PfeI GAWTC 4 cut(s) 446, 566, 974, 1856
PkrI GCNGC 1 cut(s) 19
Ple19I CGATCG 1 cut(s) 97
PsiI TTATAA 1 cut(s) 1254
Psp6I CCWGG 2 cut(s) 706, 1729
PspGI CCWGG 2 cut(s) 706, 1729
PspN4I GGNNCC 3 cut(s) 44, 705, 1402
PspPI GGNCC 2 cut(s) 77, 1058
PsuI RGATCY 2 cut(s) 592, 1169
PvuI CGATCG 1 cut(s) 97
RsaI GTAC 7 cut(s) 588, 803, 1223, 1364, 1612, 1810, 1864
RsaNI GTAC 7 cut(s) 587, 802, 1222, 1363, 1611, 1809, 1863
RseI CAYNNNNRTG 1 cut(s) 174
SaqAI TTAA 5 cut(s) 237, 438, 534, 786, 798
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 8 cut(s) 94, 139, 592, 691, 745, 865, 1169, 1243
Sau96I GGNCC 2 cut(s) 77, 1058
ScaI AGTACT 1 cut(s) 1810
ScrFI CCNGG 3 cut(s) 81, 708, 1731
SfaNI GCATC 4 cut(s) 592, 1049, 1102, 1523
SfcI CTRYAG 1 cut(s) 1907
SfuI TTCGAA 1 cut(s) 1204
SinI GGWCC 2 cut(s) 77, 1058
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 2 cut(s) 749, 1782
SmoI CTYRAG 2 cut(s) 749, 1782
SphI GCATGC 1 cut(s) 1127
SsiI CCGC 6 cut(s) 17, 103, 112, 485, 509, 1101
SspMI CTAG 9 cut(s) 273, 378, 590, 1191, 1325, 1395, 1521, 1614, 1845
StyD4I CCNGG 3 cut(s) 79, 706, 1729
StyI CCWWGG 2 cut(s) 21, 1260
TaaI ACNGT 4 cut(s) 609, 1066, 1569, 1889
TaiI ACGT 1 cut(s) 128
TaqI TCGA 2 cut(s) 133, 1204
TaqII GACCGA 2 cut(s) 87, 119
TatI WGTACW 5 cut(s) 586, 801, 1362, 1808, 1862
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 4 cut(s) 446, 566, 974, 1856
Tru1I TTAA 5 cut(s) 237, 438, 534, 786, 798
Tru9I TTAA 5 cut(s) 237, 438, 534, 786, 798
TscAI CASTG 2 cut(s) 614, 1103
TseFI GTSAC 2 cut(s) 350, 1590
Tsp45I GTSAC 2 cut(s) 350, 1590
TspDTI ATGAA 9 cut(s) 438, 456, 495, 594, 636, 657, 693, 821, 1654
TspGWI ACGGA 2 cut(s) 576, 1460
TspRI CASTG 2 cut(s) 614, 1103
VpaK11BI GGWCC 2 cut(s) 77, 1058
XapI RAATTY 2 cut(s) 300, 1432
XceI RCATGY 2 cut(s) 1127, 1874
XcmI CCANNNNNNNNNTGG 1 cut(s) 326
XmnI GAANNNNTTC 2 cut(s) 385, 630
XspI CTAG 9 cut(s) 273, 378, 590, 1191, 1325, 1395, 1521, 1614, 1845
ZrmI AGTACT 1 cut(s) 1810
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.