Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0001778

Physical Location & Seq

Genomic Coordinates Reverse (-)

28631255 .. 28633222

Gene Structure 1968 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1458 bp

ATGAGTAAGATGGGTGATAACCAGATTAACCCCCGTCAAGGTTTTGAACTCTCAGTCCCTATGCCTCCAGAAGTAGACTCCAAGTGCTATGACGACGACGGCCGTCTCAAAAGAACTGGAACTGTATGGACTGCAAGTGCTCATATTATTACTGCGGTAATTGGGTCTGGAGTTTTGTCCTTGGCTTGGGCAGTAGCTCAGCTAGGATGGGTTGCTGGTCCTGCTGTGATGCTCTTATTCTCCTTTGTCACTTACTACACTTCAACTCTTCTCTCTGCCTGCTATCGTTCCGGCGATCCTGTCACTGGAAAAAGAAACTATACTTACACCAATGCCGTCCGATCCAACCTTGGTGGGTTCAAGGTGAAAATTTGTGGGTTTGTTCAGTACTTGAATCTTTTTGGAGTTGCCATTGGATACACTATAGCATCATCCATAAGCATGGTGGCAATTAAGAGGTCTAATTGTTTCTACAAGAATGGTGACACAGCACCATGCCATGTAAACAGCAATCCTTACATGATCGCTTTTGGGGTAGCAGAAATCATATTCTCTCAAATTCCAGAGATCGATCAGCTGTGGTGGCTCTCCATTGTTGCTGCAGTCATGTCCTTCACTTATTCCTCCATCGGACTTGGCCTCGGAATCGCCAAAGTCGTAGAAACTGGAACTATCAAAGGAAGTATGACTGGAATAGACATTGGAGCAGTAACTGAAACACAAAAAATATGGAGGAGCTTCCAAGCACTTGGGGACATAGCTTTTGCCTACTCGTATTCTCTCATCCTCATTGAAATTCAGGACACGGTAAAATCCCCGCCATCCGAAGCCAAGACAATGAAGAAGGCAACTCTAGTCAGTGTGGCTACCACAACCCTTTTCTACATGTTGTGTGGTTGCATGGGCTACGCCGCTTTTGGAGACTCATCCCCTGGAAACCTCCTCACTGGTTTTGGCTTCTTCAACCCCTACTGGCTCATCGACATAGCGAACGCCGCCATTGTCATCCACCTTGTTGGTGCTTACCAAGTGTTTGTCCAACCCCTCTATGCATTTGTTGAGAAAACAGTAAAAGAAAAGTACCCGCACTGCCAGTTCATCACCAAAGACATCAAACTCCGACTCCCAGTTTTCGGTTCTTACAATCTCAACCTCTTCAGACTGGTTTGGAGGACATCTTTTGTGATTGTCACCACTGTATCGATCTCCATGATCCTGCCATTTTTCAATGATGTTGTTGCACTTCTAGGGGCTCTAGGATTTTGGCCGCTCACAGTATACTTCCCTGTGGAGATGTATATTGCTCAGAAGAGGATACCAAAGTGGAGCACAAGATGGCTTTGCCTCCAAACCTTGAGTGGTGCTTGCCTTATAATCACCATAGCAGCTGCTGCTGGATCGATTGCTGGTGTGATTAGTGATCTCAAGATCTACAAGCCTTTCAAGACCAGCTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015174 GO:0015318 GO:0015711 GO:0015802 GO:0015809 GO:0015849 GO:0016020 GO:0022857 GO:0034220 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1903825 GO:1905039 GO:1990822

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Aa_trans

Protein Analysis

486

Amino Acids

53.2

Weight (kDa)

9.04

Isoelectric Point (pI)

36.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aa_trans	PF01490	38 - 472	3.4e-120	Transmembrane amino acid transporter protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001778)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10010 AT1G10010 AT1G58360
fragaria_vesca	FvH4_2g27660 FvH4_2g27660 FvH4_2g27660 FvH4_2g27660 FvH4_2g27661 FvH4_2g27661 FvH4_5g12780
malus_domestica	MD00G1140000.v1.1 MD08G1158200.v1.1 MD15G1343500.v1.1 MD15G1343600.v1.1 MD15G1343900.v1.1
prunus_persica	Prupe.1G491000_v2.0.a1 Prupe.1G491000_v2.0.a1 Prupe.1G491000_v2.0.a1 Prupe.1G491100_v2.0.a1 Prupe.1G491200_v2.0.a1 Prupe.1G491300_v2.0.a1
pyrus_communis	pycom08g05160 pycom12419g00300 pycom15g30720
rosa_chinensis	RchiOBHm_Chr6g0296481 RchiOBHm_Chr6g0296511
rosa_laevigata	RLG00000011686 RLG00000011687 RLG00000011688
rosa_multiflora	Rmu_sc0001146.1_g000005 Rmu_sc0004318.1_g000010 Rmu_sc0006174.1_g000011
rosa_roxburghii	Rroxscaffold_7G00171480 Rroxscaffold_7G00171490 Rroxscaffold_7G00178640 Rroxscaffold_7G00178650
rosa_rugosa	Rorug06G0262800
rosa_samantha	Rh6AG374100 Rh6AG374300 Rh6BG382700 Rh6BG382800 Rh6CG387500 Rh6CG387800 Rh6DG375300 Rh6DG375500
rosa_wichuraiana	Rw6G032630 Rw6G032640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1373
AccBSI	CCGCTC	1 cut(s)	1270
AccI	GTMKAC	2 cut(s)	75, 1278
AciI	CCGC	6 cut(s)	155, 818, 912, 996, 1085, 1268
AclWI	GGATC	4 cut(s)	290, 336, 1207, 1405
AcoI	YGGCCR	2 cut(s)	100, 1265
AcsI	RAATTY	3 cut(s)	369, 558, 795
AcuI	CTGAAG	1 cut(s)	1141
AfaI	GTAC	2 cut(s)	389, 1082
AfiI	CCNNNNNNNGG	4 cut(s)	38, 186, 305, 1133
AflIII	ACRYGT	1 cut(s)	885
AgsI	TTSAA	8 cut(s)	47, 264, 361, 394, 794, 964, 1228, 1444
AjnI	CCWGG	1 cut(s)	931
AloI	GAACNNNNNNTCC	2 cut(s)	39, 71
AluBI	AGCT	7 cut(s)	197, 202, 577, 738, 761, 1388, 1452
AluI	AGCT	7 cut(s)	197, 202, 577, 738, 761, 1388, 1452
Alw21I	GWGCWC	2 cut(s)	142, 1331
Alw26I	GTCTC	2 cut(s)	110, 915
AlwI	GGATC	4 cut(s)	290, 336, 1207, 1405
AlwNI	CAGNNNCTG	3 cut(s)	713, 1391, 1455
AoxI	GGCC	3 cut(s)	100, 637, 1265
ApeKI	GCWGC	4 cut(s)	599, 1385, 1388, 1391
ApoI	RAATTY	3 cut(s)	369, 558, 795
ArsI	GACNNNNNNTTYG	2 cut(s)	746, 778
AspS9I	GGNCC	1 cut(s)	218
AsuHPI	GGTGA	6 cut(s)	26, 376, 494, 1093, 1183, 1369
AvaII	GGWCC	1 cut(s)	218
BaeI	ACNNNNGTAYC	4 cut(s)	1182, 1215, 1307, 1340
BanII	GRGCYC	1 cut(s)	1255
Bbv12I	GWGCWC	2 cut(s)	142, 1331
BbvI	GCAGC	4 cut(s)	586, 1375, 1378, 1397
BccI	CCATC	5 cut(s)	4, 201, 635, 829, 1329
BceAI	ACGGC	3 cut(s)	87, 115, 320
BciT130I	CCWGG	1 cut(s)	933
BciVI	GTATCC	2 cut(s)	410, 1308
BcoDI	GTCTC	2 cut(s)	110, 915
BfaI	CTAG	4 cut(s)	203, 854, 1247, 1256
BfmI	CTRYAG	2 cut(s)	423, 600
BfuI	GTATCC	2 cut(s)	410, 1308
BglII	AGATCT	1 cut(s)	1428
BisI	GCNGC	7 cut(s)	600, 912, 996, 1268, 1386, 1389, 1392
BlpI	GCTNAGC	1 cut(s)	198
BlsI	GCNGC	7 cut(s)	601, 913, 997, 1269, 1387, 1390, 1393
BmcAI	AGTACT	1 cut(s)	389
Bme1390I	CCNGG	1 cut(s)	933
Bme18I	GGWCC	1 cut(s)	218
BmgT120I	GGNCC	1 cut(s)	218
BmrFI	CCNGG	1 cut(s)	933
BmrI	ACTGGG	1 cut(s)	1121
BmsI	GCATC	2 cut(s)	219, 437
BmuI	ACTGGG	1 cut(s)	1121
BoxI	GACNNNNGTC	1 cut(s)	102
BpmI	CTGGAG	2 cut(s)	51, 189
Bpu1102I	GCTNAGC	1 cut(s)	198
BpuEI	CTTGAG	2 cut(s)	1375, 1409
Bsa29I	ATCGAT	3 cut(s)	570, 1202, 1400
BsaJI	CCNNGG	4 cut(s)	180, 349, 640, 931
BsaXI	ACNNNNNCTCC	2 cut(s)	1107, 1137
Bsc4I	CCNNNNNNNGG	4 cut(s)	38, 186, 305, 1133
Bse1I	ACTGG	9 cut(s)	121, 310, 670, 694, 952, 977, 1093, 1127, 1167
BseBI	CCWGG	1 cut(s)	933
BseCI	ATCGAT	3 cut(s)	570, 1202, 1400
BseDI	CCNNGG	4 cut(s)	180, 349, 640, 931
BseGI	GGATG	6 cut(s)	212, 431, 783, 821, 926, 1005
BseLI	CCNNNNNNNGG	4 cut(s)	38, 186, 305, 1133
BseMII	CTCAG	3 cut(s)	66, 212, 1319
BseNI	ACTGG	9 cut(s)	121, 310, 670, 694, 952, 977, 1093, 1127, 1167
BseRI	GAGGAG	2 cut(s)	748, 932
BseX3I	CGGCCG	1 cut(s)	100
BseXI	GCAGC	4 cut(s)	586, 1375, 1378, 1397
Bsh1285I	CGRYCG	1 cut(s)	103
BshFI	GGCC	3 cut(s)	102, 639, 1267
BshVI	ATCGAT	3 cut(s)	570, 1202, 1400
BsiEI	CGRYCG	1 cut(s)	103
BsiHKAI	GWGCWC	2 cut(s)	142, 1331
BsiSI	CCGG	1 cut(s)	291
BslFI	GGGAC	2 cut(s)	41, 767
BslI	CCNNNNNNNGG	4 cut(s)	38, 186, 305, 1133
BsmAI	GTCTC	2 cut(s)	110, 915
BsmBI	CGTCTC	1 cut(s)	110
BsmFI	GGGAC	2 cut(s)	41, 767
BsnI	GGCC	3 cut(s)	102, 639, 1267
Bsp1286I	GDGCHC	3 cut(s)	142, 1255, 1331
Bsp1720I	GCTNAGC	1 cut(s)	198
BspACI	CCGC	6 cut(s)	155, 818, 912, 996, 1085, 1268
BspANI	GGCC	3 cut(s)	102, 639, 1267
BspCNI	CTCAG	3 cut(s)	65, 211, 1318
BspDI	ATCGAT	3 cut(s)	570, 1202, 1400
BspMAI	CTGCAG	1 cut(s)	604
BspPI	GGATC	4 cut(s)	290, 336, 1207, 1405
BsrBI	CCGCTC	1 cut(s)	1270
BsrI	ACTGG	9 cut(s)	121, 310, 670, 694, 952, 977, 1093, 1127, 1167
BssECI	CCNNGG	4 cut(s)	180, 349, 640, 931
BssNAI	GTATAC	1 cut(s)	1279
BssT1I	CCWWGG	2 cut(s)	180, 349
Bst1107I	GTATAC	1 cut(s)	1279
Bst2UI	CCWGG	1 cut(s)	933
Bst4CI	ACNGT	5 cut(s)	124, 808, 1069, 1198, 1276
Bst6I	CTCTTC	3 cut(s)	273, 1160, 1304
BstAPI	GCANNNNNTGC	1 cut(s)	1391
BstC8I	GCNNGC	2 cut(s)	280, 1366
BstDEI	CTNAG	3 cut(s)	52, 198, 1305
BstF5I	GGATG	6 cut(s)	212, 431, 783, 821, 926, 1005
BstMAI	GTCTC	2 cut(s)	110, 915
BstMCI	CGRYCG	1 cut(s)	103
BstMWI	GCNNNNNNNGC	4 cut(s)	221, 583, 995, 1391
BstNI	CCWGG	1 cut(s)	933
BstNSI	RCATGY	1 cut(s)	889
BstPAI	GACNNNNGTC	1 cut(s)	102
BstSCI	CCNGG	1 cut(s)	931
BstSFI	CTRYAG	2 cut(s)	423, 600
BstV1I	GCAGC	4 cut(s)	586, 1375, 1378, 1397
BstX2I	RGATCY	1 cut(s)	1428
BstXI	CCANNNNNNTGG	3 cut(s)	442, 749, 1016
BstYI	RGATCY	1 cut(s)	1428
BstZ17I	GTATAC	1 cut(s)	1279
BstZI	CGGCCG	1 cut(s)	100
Bsu15I	ATCGAT	3 cut(s)	570, 1202, 1400
BsuI	GTATCC	2 cut(s)	410, 1308
BsuRI	GGCC	3 cut(s)	102, 639, 1267
BsuTUI	ATCGAT	3 cut(s)	570, 1202, 1400
BtsCI	GGATG	6 cut(s)	212, 431, 783, 821, 926, 1005
BtsI	GCAGTG	1 cut(s)	1087
BtsIMutI	CAGTG	5 cut(s)	303, 865, 945, 1087, 1194
Cac8I	GCNNGC	2 cut(s)	280, 1366
CaiI	CAGNNNCTG	3 cut(s)	713, 1391, 1455
Cfr13I	GGNCC	1 cut(s)	218
ClaI	ATCGAT	3 cut(s)	570, 1202, 1400
Csp6I	GTAC	2 cut(s)	388, 1081
CspCI	CAANNNNNGTGG	2 cut(s)	334, 369
CviAII	CATG	8 cut(s)	442, 495, 500, 520, 607, 886, 901, 1210
CviQI	GTAC	2 cut(s)	388, 1081
DdeI	CTNAG	3 cut(s)	52, 198, 1305
EaeI	YGGCCR	2 cut(s)	100, 1265
EagI	CGGCCG	1 cut(s)	100
Eam1104I	CTCTTC	3 cut(s)	273, 1160, 1304
EarI	CTCTTC	3 cut(s)	273, 1160, 1304
EclXI	CGGCCG	1 cut(s)	100
Eco130I	CCWWGG	2 cut(s)	180, 349
Eco24I	GRGCYC	1 cut(s)	1255
Eco47I	GGWCC	1 cut(s)	218
Eco52I	CGGCCG	1 cut(s)	100
Eco57I	CTGAAG	1 cut(s)	1141
EcoRII	CCWGG	1 cut(s)	931
EcoT14I	CCWWGG	2 cut(s)	180, 349
EcoT22I	ATGCAT	1 cut(s)	1054
EcoT38I	GRGCYC	1 cut(s)	1255
ErhI	CCWWGG	2 cut(s)	180, 349
Esp3I	CGTCTC	1 cut(s)	110
FaeI	CATG	8 cut(s)	445, 498, 503, 523, 610, 889, 904, 1213
FaqI	GGGAC	2 cut(s)	41, 767
FatI	CATG	8 cut(s)	441, 494, 499, 519, 606, 885, 900, 1209
FauI	CCCGC	2 cut(s)	825, 1092
FblI	GTMKAC	2 cut(s)	75, 1278
Fnu4HI	GCNGC	7 cut(s)	600, 912, 996, 1268, 1386, 1389, 1392
FokI	GGATG	6 cut(s)	219, 418, 770, 808, 913, 992
FriOI	GRGCYC	1 cut(s)	1255
Fsp4HI	GCNGC	7 cut(s)	600, 912, 996, 1268, 1386, 1389, 1392
FspBI	CTAG	4 cut(s)	203, 854, 1247, 1256
GluI	GCNGC	7 cut(s)	600, 912, 996, 1268, 1386, 1389, 1392
GsuI	CTGGAG	2 cut(s)	51, 189
HaeIII	GGCC	3 cut(s)	102, 639, 1267
HapII	CCGG	1 cut(s)	291
Hin1II	CATG	8 cut(s)	445, 498, 503, 523, 610, 889, 904, 1213
HinfI	GANTC	5 cut(s)	77, 394, 645, 923, 1122
HpaII	CCGG	1 cut(s)	291
HphI	GGTGA	6 cut(s)	26, 376, 494, 1093, 1183, 1369
Hpy166II	GTNNAC	3 cut(s)	76, 505, 1279
Hpy188I	TCNGA	7 cut(s)	341, 632, 644, 826, 1121, 1160, 1308
Hpy188III	TCNNGA	6 cut(s)	68, 168, 563, 800, 1426, 1444
Hpy8I	GTNNAC	3 cut(s)	76, 505, 1279
Hpy99I	CGWCG	2 cut(s)	98, 101
HpyAV	CCTTC	2 cut(s)	622, 838
HpyCH4III	ACNGT	5 cut(s)	124, 808, 1069, 1198, 1276
HpyCH4V	TGCA	5 cut(s)	134, 602, 900, 1052, 1241
HpyF10VI	GCNNNNNNNGC	4 cut(s)	221, 583, 995, 1391
HpyF3I	CTNAG	3 cut(s)	52, 198, 1305
Hsp92II	CATG	8 cut(s)	445, 498, 503, 523, 610, 889, 904, 1213
LmnI	GCTCC	3 cut(s)	704, 735, 1326
Lsp1109I	GCAGC	4 cut(s)	586, 1375, 1378, 1397
LweI	GCATC	2 cut(s)	219, 437
MaeI	CTAG	4 cut(s)	203, 854, 1247, 1256
MaeIII	GTNAC	5 cut(s)	247, 301, 482, 709, 1189
MbiI	CCGCTC	1 cut(s)	1270
MboII	GAAGA	5 cut(s)	260, 853, 952, 1147, 1321
MflI	RGATCY	1 cut(s)	1428
MhlI	GDGCHC	3 cut(s)	142, 1255, 1331
MluCI	AATT	6 cut(s)	159, 369, 450, 463, 558, 795
MlyI	GAGTC	3 cut(s)	71, 917, 1116
MmeI	TCCRAC	3 cut(s)	369, 1063, 1144
Mph1103I	ATGCAT	1 cut(s)	1054
MseI	TTAA	2 cut(s)	27, 453
MslI	CAYNNNNRTG	1 cut(s)	440
MspA1I	CMGCKG	2 cut(s)	577, 1388
MspI	CCGG	1 cut(s)	291
MspR9I	CCNGG	1 cut(s)	933
MvaI	CCWGG	1 cut(s)	933
MwoI	GCNNNNNNNGC	4 cut(s)	221, 583, 995, 1391
NlaIII	CATG	8 cut(s)	445, 498, 503, 523, 610, 889, 904, 1213
NmuCI	GTSAC	4 cut(s)	247, 301, 482, 1189
NsiI	ATGCAT	1 cut(s)	1054
NspI	RCATGY	1 cut(s)	889
PciI	ACATGT	1 cut(s)	885
PcsI	WCGNNNNNNNCGW	1 cut(s)	654
PfeI	GAWTC	2 cut(s)	394, 645
PkrI	GCNGC	7 cut(s)	601, 913, 997, 1269, 1387, 1390, 1393
PleI	GAGTC	3 cut(s)	71, 917, 1116
PpsI	GAGTC	3 cut(s)	71, 917, 1116
PscI	ACATGT	1 cut(s)	885
PshAI	GACNNNNGTC	1 cut(s)	102
PsiI	TTATAA	1 cut(s)	1373
Psp6I	CCWGG	1 cut(s)	931
PspGI	CCWGG	1 cut(s)	931
PspPI	GGNCC	1 cut(s)	218
PstI	CTGCAG	1 cut(s)	604
PstNI	CAGNNNCTG	3 cut(s)	713, 1391, 1455
PsuI	RGATCY	1 cut(s)	1428
PvuII	CAGCTG	2 cut(s)	577, 1388
RsaI	GTAC	2 cut(s)	389, 1082
RsaNI	GTAC	2 cut(s)	388, 1081
RseI	CAYNNNNRTG	1 cut(s)	440
SaqAI	TTAA	2 cut(s)	27, 453
SatI	GCNGC	7 cut(s)	600, 912, 996, 1268, 1386, 1389, 1392
Sau96I	GGNCC	1 cut(s)	218
ScaI	AGTACT	1 cut(s)	389
SchI	GAGTC	3 cut(s)	71, 917, 1116
ScrFI	CCNGG	1 cut(s)	933
SduI	GDGCHC	3 cut(s)	142, 1255, 1331
SfaNI	GCATC	2 cut(s)	219, 437
SfcI	CTRYAG	2 cut(s)	423, 600
SinI	GGWCC	1 cut(s)	218
SmiMI	CAYNNNNRTG	1 cut(s)	440
SmlI	CTYRAG	2 cut(s)	1354, 1424
SmoI	CTYRAG	2 cut(s)	1354, 1424
Sse9I	AATT	6 cut(s)	159, 369, 450, 463, 558, 795
SsiI	CCGC	6 cut(s)	155, 818, 912, 996, 1085, 1268
SspMI	CTAG	4 cut(s)	203, 854, 1247, 1256
StyD4I	CCNGG	1 cut(s)	931
StyI	CCWWGG	2 cut(s)	180, 349
TaaI	ACNGT	5 cut(s)	124, 808, 1069, 1198, 1276
TaqI	TCGA	4 cut(s)	570, 981, 1202, 1400
TasI	AATT	6 cut(s)	159, 369, 450, 463, 558, 795
TatI	WGTACW	1 cut(s)	387
TauI	GCSGC	3 cut(s)	914, 998, 1270
TfiI	GAWTC	2 cut(s)	394, 645
Tru1I	TTAA	2 cut(s)	27, 453
Tru9I	TTAA	2 cut(s)	27, 453
TscAI	CASTG	5 cut(s)	310, 865, 952, 1094, 1201
TseFI	GTSAC	4 cut(s)	247, 301, 482, 1189
TseI	GCWGC	4 cut(s)	599, 1385, 1388, 1391
Tsp45I	GTSAC	4 cut(s)	247, 301, 482, 1189
TspDTI	ATGAA	2 cut(s)	854, 1087
TspRI	CASTG	5 cut(s)	310, 865, 952, 1094, 1201
VpaK11BI	GGWCC	1 cut(s)	218
XapI	RAATTY	3 cut(s)	369, 558, 795
XceI	RCATGY	1 cut(s)	889
XcmI	CCANNNNNNNNNTGG	2 cut(s)	442, 1355
XmiI	GTMKAC	2 cut(s)	75, 1278
XspI	CTAG	4 cut(s)	203, 854, 1247, 1256
ZrmI	AGTACT	1 cut(s)	389
Zsp2I	ATGCAT	1 cut(s)	1054

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.