pycom16g01140

D-galacturonate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003272
Physical Location & Seq
Reverse (-)
702379 .. 703921
1543 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 957 bp
ATGACGCCAATGATAGTTCCTGAGGTAACACTGAGCTCCTGCGACACGACGATGCCAGTGATCGGCATGGGGACTTCATCGTACCCTAAAGTTGACCCTGAAACAGCCAAGGCTGCAATTCCTGAAGCCATCAGAGCGGGCTACCGCCACTTCGACACTGCCTTTGCATACGGGTCAGAGCAAGATCTGGGGGAAGCCATAGCTGAAGCACTGCGTCTTGGACTCGTCAAGTCCAGGACCGAGCTCTTCATCACCACCAAGCTTTTTGCCAGCTTTGCTGAAAGAGAGCTTGTAGTGCCTGCCATCAACAAGAGTTTAAGGAATCTGCAATTGGAGTATGTGGATATGTACCTAATACATTGGCCCTTCAAATTTGGGAAAGTGGTGAGCTCTATGCCAGTGGAAAAGGGGGTGCTGCTACCCTTAGACATCAAATCAGTTTGGGAAGGCATGGAAGAATGCAAGAGATTGGGCCTTGCGAGGGGCATTGGCGTGAGTAACTTCACTTCCGAGATGCTTGAGGACCTCCTTTCCATCGCCAAAATCCCTCCCGCCCTCAACCAATTGGAGATGAACCCACTTTGGCAGCTGAAGAAACTGAGGGAGTTCTGTAAGGCAAAGGGCATTCATGTGACGGCCTACTCTCCGTTAGGTTCAACCGGGACTAAATTTGGAGGCAACAAAGTCTTAGGCTCGAAAGTCCTCCAAGACATTGCCGAATCCAAAGGAAAAACAACTGCTCAGATAGCGTTGAGATGGGTGTACCAGCAAGGGGTGAGCTTGGTAACAAAGAGCGTCAATAAGGAAAGAATGAATCAGAACATTGACATCTTCGGTTGGTCCTTGACTGAGGAGGAATTAGACGAGATTAGTCGTCTTCCTCAACACAAAACAATCACCTTCGCCTCAATTATGGGACCGCATGATGTTGTGCTCCAGGTCGATGCTGGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 map00053
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

319

Amino Acids

35.16

Weight (kDa)

6.55

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 21 - 292 4.8e-54 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 137
AciI CCGC 4 cut(s) 137, 145, 552, 920
AcsI RAATTY 2 cut(s) 371, 668
AcuI CTGAAG 3 cut(s) 144, 225, 611
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 83, 350, 764
AfiI CCNNNNNNNGG 3 cut(s) 62, 481, 772
AgsI TTSAA 2 cut(s) 370, 657
AjnI CCWGG 2 cut(s) 233, 936
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
AluBI AGCT 9 cut(s) 36, 203, 244, 262, 273, 289, 390, 589, 780
AluI AGCT 9 cut(s) 36, 203, 244, 262, 273, 289, 390, 589, 780
Alw21I GWGCWC 4 cut(s) 38, 246, 392, 936
AoxI GGCC 3 cut(s) 362, 472, 636
ApeKI GCWGC 3 cut(s) 113, 415, 586
ApoI RAATTY 2 cut(s) 371, 668
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AspS9I GGNCC 6 cut(s) 237, 363, 472, 523, 840, 917
AsuC2I CCSGG 1 cut(s) 661
AsuHPI GGTGA 4 cut(s) 244, 397, 787, 889
AvaII GGWCC 4 cut(s) 237, 523, 840, 917
AxyI CCTNAGG 1 cut(s) 21
BanII GRGCYC 3 cut(s) 38, 246, 392
BarI GAAGNNNNNNTAC 2 cut(s) 490, 522
BbsI GAAGAC 1 cut(s) 869
Bbv12I GWGCWC 4 cut(s) 38, 246, 392, 936
BbvI GCAGC 3 cut(s) 100, 402, 598
BccI CCATC 4 cut(s) 137, 311, 542, 750
BceAI ACGGC 1 cut(s) 651
BciT130I CCWGG 2 cut(s) 235, 938
BcnI CCSGG 1 cut(s) 661
BglII AGATCT 1 cut(s) 184
BisI GCNGC 3 cut(s) 114, 416, 587
BlsI GCNGC 3 cut(s) 115, 417, 588
Bme1390I CCNGG 3 cut(s) 235, 661, 938
Bme18I GGWCC 4 cut(s) 237, 523, 840, 917
BmgT120I GGNCC 6 cut(s) 237, 363, 472, 523, 840, 917
BmiI GGNNCC 1 cut(s) 918
BmrFI CCNGG 3 cut(s) 235, 661, 938
BmsI GCATC 3 cut(s) 42, 504, 934
BpiI GAAGAC 1 cut(s) 869
BpmI CTGGAG 1 cut(s) 920
BpuEI CTTGAG 1 cut(s) 539
BpuMI CCSGG 1 cut(s) 661
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 481, 772
Bse1I ACTGG 2 cut(s) 56, 398
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 711
BseBI CCWGG 2 cut(s) 235, 938
BseDI CCNNGG 1 cut(s) 108
BseLI CCNNNNNNNGG 3 cut(s) 62, 481, 772
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 5 cut(s) 12, 23, 590, 755, 840
BseNI ACTGG 2 cut(s) 56, 398
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 3 cut(s) 100, 402, 598
BshFI GGCC 3 cut(s) 364, 474, 638
BsiHKAI GWGCWC 4 cut(s) 38, 246, 392, 936
BsiSI CCGG 1 cut(s) 660
BslFI GGGAC 3 cut(s) 85, 676, 930
BslI CCNNNNNNNGG 3 cut(s) 62, 481, 772
BsmFI GGGAC 3 cut(s) 85, 676, 930
BsmI GAATGC 2 cut(s) 464, 624
BsnI GGCC 3 cut(s) 364, 474, 638
Bsp1286I GDGCHC 4 cut(s) 38, 246, 392, 936
Bsp143I GATC 2 cut(s) 60, 184
BspACI CCGC 4 cut(s) 137, 145, 552, 920
BspANI GGCC 3 cut(s) 364, 474, 638
BspCNI CTCAG 5 cut(s) 13, 24, 591, 754, 841
BspLI GGNNCC 1 cut(s) 918
BspQI GCTCTTC 1 cut(s) 251
BsrBI CCGCTC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 711
BsrI ACTGG 2 cut(s) 56, 398
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 2 cut(s) 60, 184
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 2 cut(s) 235, 938
Bst6I CTCTTC 1 cut(s) 251
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 3 cut(s) 139, 271, 300
BstDEI CTNAG 7 cut(s) 21, 32, 424, 599, 688, 741, 849
BstKTI GATC 2 cut(s) 63, 187
BstMBI GATC 2 cut(s) 60, 184
BstMWI GCNNNNNNNGC 5 cut(s) 113, 134, 275, 295, 746
BstNI CCWGG 2 cut(s) 235, 938
BstSCI CCNGG 3 cut(s) 233, 659, 936
BstV1I GCAGC 3 cut(s) 100, 402, 598
BstV2I GAAGAC 1 cut(s) 869
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 3 cut(s) 364, 474, 638
BtgZI GCGATG 1 cut(s) 520
BtsI GCAGTG 2 cut(s) 156, 209
BtsIMutI CAGTG 5 cut(s) 29, 63, 156, 209, 405
Cac8I GCNNGC 3 cut(s) 139, 271, 300
Cfr13I GGNCC 6 cut(s) 237, 363, 472, 523, 840, 917
CseI GACGC 3 cut(s) 13, 203, 784
Csp6I GTAC 3 cut(s) 82, 349, 763
CviAII CATG 4 cut(s) 67, 451, 629, 923
CviQI GTAC 3 cut(s) 82, 349, 763
DdeI CTNAG 7 cut(s) 21, 32, 424, 599, 688, 741, 849
DpnI GATC 2 cut(s) 62, 186
DpnII GATC 2 cut(s) 60, 184
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Ecl136II GAGCTC 3 cut(s) 36, 244, 390
Eco130I CCWWGG 1 cut(s) 108
Eco24I GRGCYC 3 cut(s) 38, 246, 392
Eco47I GGWCC 4 cut(s) 237, 523, 840, 917
Eco53kI GAGCTC 3 cut(s) 36, 244, 390
Eco57I CTGAAG 3 cut(s) 144, 225, 611
Eco81I CCTNAGG 1 cut(s) 21
EcoICRI GAGCTC 3 cut(s) 36, 244, 390
EcoO109I RGGNCCY 1 cut(s) 523
EcoRII CCWGG 2 cut(s) 233, 936
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 3 cut(s) 38, 246, 392
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 4 cut(s) 70, 454, 632, 926
FaqI GGGAC 3 cut(s) 85, 676, 930
FatI CATG 4 cut(s) 66, 450, 628, 922
FauI CCCGC 2 cut(s) 130, 559
Fnu4HI GCNGC 3 cut(s) 114, 416, 587
FriOI GRGCYC 3 cut(s) 38, 246, 392
Fsp4HI GCNGC 3 cut(s) 114, 416, 587
GluI GCNGC 3 cut(s) 114, 416, 587
GsuI CTGGAG 1 cut(s) 920
HaeIII GGCC 3 cut(s) 364, 474, 638
HapII CCGG 1 cut(s) 660
HgaI GACGC 3 cut(s) 13, 203, 784
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 70, 454, 632, 926
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 4 cut(s) 222, 322, 719, 814
HpaII CCGG 1 cut(s) 660
HphI GGTGA 4 cut(s) 244, 397, 787, 889
Hpy166II GTNNAC 2 cut(s) 94, 763
Hpy188I TCNGA 5 cut(s) 134, 178, 511, 744, 819
Hpy188III TCNNGA 2 cut(s) 20, 122
Hpy8I GTNNAC 2 cut(s) 94, 763
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 3 cut(s) 376, 440, 910
HpyCH4V TGCA 4 cut(s) 116, 167, 328, 462
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 134, 275, 295, 746
HpyF3I CTNAG 7 cut(s) 21, 32, 424, 599, 688, 741, 849
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 70, 454, 632, 926
Kzo9I GATC 2 cut(s) 60, 184
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 2 cut(s) 41, 939
Lsp1109I GCAGC 3 cut(s) 100, 402, 598
LweI GCATC 3 cut(s) 42, 504, 934
MaeIII GTNAC 4 cut(s) 25, 497, 631, 784
MalI GATC 2 cut(s) 62, 186
MbiI CCGCTC 1 cut(s) 137
MboI GATC 2 cut(s) 60, 184
MboII GAAGA 5 cut(s) 238, 467, 604, 823, 869
MfeI CAATTG 2 cut(s) 329, 563
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 4 cut(s) 38, 246, 392, 936
MluCI AATT 7 cut(s) 117, 329, 371, 563, 668, 857, 909
MlyI GAGTC 1 cut(s) 216
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 3 cut(s) 50, 491, 629
MspA1I CMGCKG 1 cut(s) 589
MspI CCGG 1 cut(s) 660
MspR9I CCNGG 3 cut(s) 235, 661, 938
MunI CAATTG 2 cut(s) 329, 563
Mva1269I GAATGC 2 cut(s) 464, 624
MvaI CCWGG 2 cut(s) 235, 938
MwoI GCNNNNNNNGC 5 cut(s) 113, 134, 275, 295, 746
NciI CCSGG 1 cut(s) 661
NdeII GATC 2 cut(s) 60, 184
NlaIII CATG 4 cut(s) 70, 454, 632, 926
NlaIV GGNNCC 1 cut(s) 918
NmuCI GTSAC 1 cut(s) 631
PciSI GCTCTTC 1 cut(s) 251
PctI GAATGC 2 cut(s) 464, 624
PfeI GAWTC 3 cut(s) 322, 719, 814
PfoI TCCNGGA 1 cut(s) 233
PkrI GCNGC 3 cut(s) 115, 417, 588
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
PpuMI RGGWCCY 1 cut(s) 523
Psp124BI GAGCTC 3 cut(s) 38, 246, 392
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 2 cut(s) 233, 936
PspGI CCWGG 2 cut(s) 233, 936
PspN4I GGNNCC 1 cut(s) 918
PspPI GGNCC 6 cut(s) 237, 363, 472, 523, 840, 917
PspPPI RGGWCCY 1 cut(s) 523
PsuI RGATCY 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 589
RsaI GTAC 3 cut(s) 83, 350, 764
RsaNI GTAC 3 cut(s) 82, 349, 763
RseI CAYNNNNRTG 3 cut(s) 50, 491, 629
SacI GAGCTC 3 cut(s) 38, 246, 392
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 3 cut(s) 114, 416, 587
Sau3AI GATC 2 cut(s) 60, 184
Sau96I GGNCC 6 cut(s) 237, 363, 472, 523, 840, 917
SchI GAGTC 1 cut(s) 216
ScrFI CCNGG 3 cut(s) 235, 661, 938
SduI GDGCHC 4 cut(s) 38, 246, 392, 936
SfaNI GCATC 3 cut(s) 42, 504, 934
SinI GGWCC 4 cut(s) 237, 523, 840, 917
SmiMI CAYNNNNRTG 3 cut(s) 50, 491, 629
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
Sse9I AATT 7 cut(s) 117, 329, 371, 563, 668, 857, 909
SsiI CCGC 4 cut(s) 137, 145, 552, 920
SstI GAGCTC 3 cut(s) 38, 246, 392
StyD4I CCNGG 3 cut(s) 233, 659, 936
StyI CCWWGG 1 cut(s) 108
TaqI TCGA 3 cut(s) 153, 695, 942
TaqII GACCGA 1 cut(s) 254
TasI AATT 7 cut(s) 117, 329, 371, 563, 668, 857, 909
TfiI GAWTC 3 cut(s) 322, 719, 814
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 5 cut(s) 36, 63, 163, 216, 405
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 3 cut(s) 113, 415, 586
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 5 cut(s) 66, 238, 587, 617, 827
TspGWI ACGGA 1 cut(s) 636
TspRI CASTG 5 cut(s) 36, 63, 163, 216, 405
VpaK11BI GGWCC 4 cut(s) 237, 523, 840, 917
XapI RAATTY 2 cut(s) 371, 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.