pycom16g11370

Emopamil binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004072
Physical Location & Seq
Forward (+)
7936438 .. 7937874
1437 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 711 bp
ATGGAGGGATCGGACGCTGGAATTTATGGGAATCATCCGTACGCCCCGAGGGATCGATCATTACTACATAAGTTAAAGCTACCGGGATTCGTACCTGGCTTTCTCTCACAGTCCACCATTGTCGGCGTCTACGGCGTCTCCTCCCTACTCGTCGTTTCTCTCACCTGGCTATTCTCCGGGAGGTCACCGAGGAAATCAAAACTTGATAGATGGCTAATGTGCTGGTGGGCTTTCACCGGCCTTACACACTTGATACTCGAAGGCTATTTTGCATTTGCGCCTGAGTTCTACAAGGATGAGACTGCTTGGTACCCAGCTGAAGTTTGGAAAGAGTATAGCAAAGGTGATTCAAGATATGCAGCTAGGGATGCAGGCGTCGTTGCTGTCGAAGGATTAACTGCGGTTATTGAAGGTCCCGCTAGCCTTCTTGCTGTGTATGCAATATCTAAAGGCAAGTCATATAGCTACATACTTCAGTTTGCCATTTCGTTGGGTCAGCTCTATGGAACTGCTGTGTACTTCATAACAGCCTACTTGGACGGTGACAAATTTGCTACAAGTTCGTTTTACTACTATGCATACTACATTGCTGCAAACGCCTCCTGGGTTGTAATACCGACACTCATTTCCATCCGCTGTTGGAAGAAGATTTCTGCCGCAGTACAAGTTCAAGGCCAAGGCCAAGGCCAGAAAAAGAACAAAACTCGCTGA

Protein Analysis

237

Amino Acids

26.28

Weight (kDa)

9.3

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EBP PF05241 106 - 213 2.9e-22 EXPERA (EXPanded EBP superfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 309
AccB1I GGYRCC 1 cut(s) 309
AccI GTMKAC 1 cut(s) 129
AciI CCGC 4 cut(s) 401, 417, 634, 657
AclWI GGATC 2 cut(s) 16, 60
AcsI RAATTY 2 cut(s) 21, 548
AcuI CTGAAG 2 cut(s) 339, 458
AcyI GRCGYC 3 cut(s) 126, 135, 375
AfaI GTAC 5 cut(s) 41, 93, 311, 518, 663
AgsI TTSAA 3 cut(s) 351, 410, 671
AjnI CCWGG 3 cut(s) 94, 164, 602
AluBI AGCT 5 cut(s) 79, 317, 362, 465, 499
AluI AGCT 5 cut(s) 79, 317, 362, 465, 499
Alw26I GTCTC 2 cut(s) 142, 293
AlwI GGATC 2 cut(s) 16, 60
Ama87I CYCGRG 1 cut(s) 46
AoxI GGCC 4 cut(s) 238, 673, 679, 685
ApeKI GCWGC 2 cut(s) 359, 590
ApoI RAATTY 2 cut(s) 21, 548
Asp718I GGTACC 1 cut(s) 309
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 1 cut(s) 413
AsuC2I CCSGG 2 cut(s) 84, 178
AsuHPI GGTGA 5 cut(s) 154, 177, 226, 356, 554
AsuNHI GCTAGC 1 cut(s) 419
AvaI CYCGRG 1 cut(s) 46
AvaII GGWCC 1 cut(s) 413
BanI GGYRCC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 371, 577
BccI CCATC 2 cut(s) 204, 638
BceAI ACGGC 1 cut(s) 148
BciT130I CCWGG 3 cut(s) 96, 166, 604
BcnI CCSGG 2 cut(s) 84, 178
BcoDI GTCTC 2 cut(s) 142, 293
BfaI CTAG 2 cut(s) 363, 420
BisI GCNGC 3 cut(s) 360, 591, 657
BlsI GCNGC 3 cut(s) 361, 592, 658
Bme1390I CCNGG 5 cut(s) 84, 96, 166, 178, 604
Bme18I GGWCC 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 413
BmiI GGNNCC 2 cut(s) 311, 415
BmrFI CCNGG 5 cut(s) 84, 96, 166, 178, 604
BmsI GCATC 1 cut(s) 358
BmtI GCTAGC 1 cut(s) 423
BpuMI CCSGG 2 cut(s) 84, 178
Bsa29I ATCGAT 1 cut(s) 55
BsaHI GRCGYC 3 cut(s) 126, 135, 375
BsaJI CCNNGG 5 cut(s) 47, 188, 603, 676, 682
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse118I RCCGGY 1 cut(s) 236
Bse3DI GCAATG 1 cut(s) 585
BseBI CCWGG 3 cut(s) 96, 166, 604
BseCI ATCGAT 1 cut(s) 55
BseDI CCNNGG 5 cut(s) 47, 188, 603, 676, 682
BseGI GGATG 4 cut(s) 34, 301, 373, 630
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 1 cut(s) 273
BseRI GAGGAG 1 cut(s) 130
BseXI GCAGC 2 cut(s) 371, 577
BseYI CCCAGC 1 cut(s) 313
BshFI GGCC 4 cut(s) 240, 675, 681, 687
BshNI GGYRCC 1 cut(s) 309
BshVI ATCGAT 1 cut(s) 55
BsiHKCI CYCGRG 1 cut(s) 46
BsiSI CCGG 3 cut(s) 83, 177, 237
BsiWI CGTACG 1 cut(s) 39
BslFI GGGAC 1 cut(s) 399
BsmAI GTCTC 2 cut(s) 142, 293
BsmBI CGTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 399
BsnI GGCC 4 cut(s) 240, 675, 681, 687
BsoBI CYCGRG 1 cut(s) 46
Bsp143I GATC 3 cut(s) 8, 52, 56
BspACI CCGC 4 cut(s) 401, 417, 634, 657
BspANI GGCC 4 cut(s) 240, 675, 681, 687
BspCNI CTCAG 1 cut(s) 274
BspDI ATCGAT 1 cut(s) 55
BspLI GGNNCC 2 cut(s) 311, 415
BspOI GCTAGC 1 cut(s) 423
BspPI GGATC 2 cut(s) 16, 60
BspT107I GGYRCC 1 cut(s) 309
BsrDI GCAATG 1 cut(s) 585
BsrFI RCCGGY 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 5 cut(s) 47, 188, 603, 676, 682
BssMI GATC 3 cut(s) 8, 52, 56
BssNI GRCGYC 3 cut(s) 126, 135, 375
BssT1I CCWWGG 2 cut(s) 676, 682
Bst2UI CCWGG 3 cut(s) 96, 166, 604
Bst4CI ACNGT 2 cut(s) 111, 542
BstACI GRCGYC 3 cut(s) 126, 135, 375
BstC8I GCNNGC 2 cut(s) 373, 421
BstDEI CTNAG 1 cut(s) 282
BstEII GGTNACC 1 cut(s) 183
BstF5I GGATG 4 cut(s) 34, 301, 373, 630
BstHHI GCGC 1 cut(s) 280
BstKTI GATC 3 cut(s) 11, 55, 59
BstMAI GTCTC 2 cut(s) 142, 293
BstMBI GATC 3 cut(s) 8, 52, 56
BstMWI GCNNNNNNNGC 4 cut(s) 132, 368, 437, 596
BstNI CCWGG 3 cut(s) 96, 166, 604
BstPI GGTNACC 1 cut(s) 183
BstSCI CCNGG 5 cut(s) 82, 94, 164, 176, 602
BstV1I GCAGC 2 cut(s) 371, 577
BstXI CCANNNNNNTGG 1 cut(s) 490
Bsu15I ATCGAT 1 cut(s) 55
BsuRI GGCC 4 cut(s) 240, 675, 681, 687
BsuTUI ATCGAT 1 cut(s) 55
BtsCI GGATG 4 cut(s) 34, 301, 373, 630
Cac8I GCNNGC 2 cut(s) 373, 421
CfoI GCGC 1 cut(s) 280
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 413
ClaI ATCGAT 1 cut(s) 55
CseI GACGC 4 cut(s) 23, 115, 124, 364
Csp6I GTAC 5 cut(s) 40, 92, 310, 517, 662
CviQI GTAC 5 cut(s) 40, 92, 310, 517, 662
DdeI CTNAG 1 cut(s) 282
DpnI GATC 3 cut(s) 10, 54, 58
DpnII GATC 3 cut(s) 8, 52, 56
Eco130I CCWWGG 2 cut(s) 676, 682
Eco47I GGWCC 1 cut(s) 413
Eco57I CTGAAG 2 cut(s) 339, 458
Eco88I CYCGRG 1 cut(s) 46
Eco91I GGTNACC 1 cut(s) 183
EcoO109I RGGNCCY 1 cut(s) 413
EcoO65I GGTNACC 1 cut(s) 183
EcoRII CCWGG 3 cut(s) 94, 164, 602
EcoT14I CCWWGG 2 cut(s) 676, 682
EcoT22I ATGCAT 1 cut(s) 580
ErhI CCWWGG 2 cut(s) 676, 682
Esp3I CGTCTC 1 cut(s) 142
FaqI GGGAC 1 cut(s) 399
FauI CCCGC 1 cut(s) 424
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 3 cut(s) 360, 591, 657
FokI GGATG 4 cut(s) 21, 308, 380, 617
Fsp4HI GCNGC 3 cut(s) 360, 591, 657
FspBI CTAG 2 cut(s) 363, 420
GlaI GCGC 1 cut(s) 279
GluI GCNGC 3 cut(s) 360, 591, 657
GsaI CCCAGC 1 cut(s) 317
HaeIII GGCC 4 cut(s) 240, 675, 681, 687
HapII CCGG 3 cut(s) 83, 177, 237
HgaI GACGC 4 cut(s) 23, 115, 124, 364
HhaI GCGC 1 cut(s) 280
Hin1I GRCGYC 3 cut(s) 126, 135, 375
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HinfI GANTC 3 cut(s) 31, 87, 347
HpaII CCGG 3 cut(s) 83, 177, 237
HphI GGTGA 5 cut(s) 154, 177, 226, 356, 554
Hpy166II GTNNAC 3 cut(s) 114, 130, 517
Hpy188I TCNGA 1 cut(s) 13
Hpy188III TCNNGA 1 cut(s) 351
Hpy8I GTNNAC 3 cut(s) 114, 130, 517
Hpy99I CGWCG 2 cut(s) 155, 380
HpyAV CCTTC 4 cut(s) 254, 383, 404, 434
HpyCH4III ACNGT 2 cut(s) 111, 542
HpyCH4V TGCA 6 cut(s) 272, 359, 371, 440, 578, 593
HpyF10VI GCNNNNNNNGC 4 cut(s) 132, 368, 437, 596
HpyF3I CTNAG 1 cut(s) 282
Hsp92I GRCGYC 3 cut(s) 126, 135, 375
HspAI GCGC 1 cut(s) 278
KpnI GGTACC 1 cut(s) 313
Kzo9I GATC 3 cut(s) 8, 52, 56
Lsp1109I GCAGC 2 cut(s) 371, 577
LweI GCATC 1 cut(s) 358
MaeI CTAG 2 cut(s) 363, 420
MaeIII GTNAC 2 cut(s) 183, 542
MalI GATC 3 cut(s) 10, 54, 58
MboI GATC 3 cut(s) 8, 52, 56
MboII GAAGA 2 cut(s) 655, 658
MluCI AATT 2 cut(s) 21, 548
MmeI TCCRAC 1 cut(s) 620
MnlI CCTC 5 cut(s) 42, 151, 174, 183, 610
Mph1103I ATGCAT 1 cut(s) 580
MseI TTAA 2 cut(s) 74, 395
MspA1I CMGCKG 2 cut(s) 317, 636
MspI CCGG 3 cut(s) 83, 177, 237
MspR9I CCNGG 5 cut(s) 84, 96, 166, 178, 604
MvaI CCWGG 3 cut(s) 96, 166, 604
MwoI GCNNNNNNNGC 4 cut(s) 132, 368, 437, 596
NciI CCSGG 2 cut(s) 84, 178
NdeII GATC 3 cut(s) 8, 52, 56
NheI GCTAGC 1 cut(s) 419
NlaIV GGNNCC 2 cut(s) 311, 415
NmuCI GTSAC 2 cut(s) 183, 542
NsiI ATGCAT 1 cut(s) 580
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PfeI GAWTC 3 cut(s) 31, 87, 347
Pfl23II CGTACG 1 cut(s) 39
PfoI TCCNGGA 1 cut(s) 176
PkrI GCNGC 3 cut(s) 361, 592, 658
PpuMI RGGWCCY 1 cut(s) 413
Psp5II RGGWCCY 1 cut(s) 413
Psp6I CCWGG 3 cut(s) 94, 164, 602
PspEI GGTNACC 1 cut(s) 183
PspFI CCCAGC 1 cut(s) 313
PspGI CCWGG 3 cut(s) 94, 164, 602
PspLI CGTACG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 311, 415
PspPI GGNCC 1 cut(s) 413
PspPPI RGGWCCY 1 cut(s) 413
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 5 cut(s) 41, 93, 311, 518, 663
RsaNI GTAC 5 cut(s) 40, 92, 310, 517, 662
SaqAI TTAA 2 cut(s) 74, 395
SatI GCNGC 3 cut(s) 360, 591, 657
Sau3AI GATC 3 cut(s) 8, 52, 56
Sau96I GGNCC 1 cut(s) 413
ScrFI CCNGG 5 cut(s) 84, 96, 166, 178, 604
SfaNI GCATC 1 cut(s) 358
SinI GGWCC 1 cut(s) 413
Sse9I AATT 2 cut(s) 21, 548
SsiI CCGC 4 cut(s) 401, 417, 634, 657
SspMI CTAG 2 cut(s) 363, 420
StyD4I CCNGG 5 cut(s) 82, 94, 164, 176, 602
StyI CCWWGG 2 cut(s) 676, 682
TaaI ACNGT 2 cut(s) 111, 542
TaqI TCGA 3 cut(s) 55, 258, 387
TasI AATT 2 cut(s) 21, 548
TatI WGTACW 2 cut(s) 516, 661
TauI GCSGC 1 cut(s) 659
TfiI GAWTC 3 cut(s) 31, 87, 347
Tru1I TTAA 2 cut(s) 74, 395
Tru9I TTAA 2 cut(s) 74, 395
TseFI GTSAC 2 cut(s) 183, 542
TseI GCWGC 2 cut(s) 359, 590
Tsp45I GTSAC 2 cut(s) 183, 542
TspDTI ATGAA 1 cut(s) 511
TspGWI ACGGA 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 413
XapI RAATTY 2 cut(s) 21, 548
XcmI CCANNNNNNNNNTGG 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 2 cut(s) 363, 420
Zsp2I ATGCAT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.