pycom17g10850

Transmembrane emp24 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004137
Physical Location & Seq
Reverse (-)
8436911 .. 8438355
1445 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 651 bp
ATGATTTTAGGGATTTGTCCTTTTATTCTGGTCTGGTTCAACAAGAAGTGCCTGGTCCGACAGCCGAGTTCGAGTTCGGCACTTCTCAAATCCCAGTTCATCCTAAAATCCCAAGAACTTCAAACCTCCGCCAAATACAAAACTAGAACCTATTTCCGAGGGAGGAAGATGGAGTTCTGGGGCGGAGGACACGTGTCCCTGAGTTTGGTGCTGGTGGGTTTTCTGGTTCTGTGCCAGTTAGAAGGGTCGTCAGGGTTGAGGTTCGTGATAGACAGGGAAGAGTGCTTTTCTCATGATGCAAAGTACATGGGAGACACGATTCACGTCTCTTTCGTGGTAATCAAAGTCGATTCGACCTGGCATTACACCGACGACGGCGTTGATCTCGTGATAAAGGGACCTGGTGGCGAACAAATTCATGACATACGCGACAAGACAAGCGAGAAGTATGACCTTGTGGTTCAGAAGCAAGGTGTTCACCAGTTCTGCTTCACTAACAAGTCTCCTTACCACGAAACTATTGACTTTGATGTACATGCTGCTCACTTTACATACCATGACCAGCATGCTAAAGACGGTGAGAGGCCATCACTTGTTATTACCATTTCCGTTATGGATGAGTCTTCTTTATGGCGGAATAATGATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EMP24_GP25L

Protein Analysis

217

Amino Acids

24.72

Weight (kDa)

6.62

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMP24_GP25L PF01105 92 - 187 1.7e-13 emp24/gp25L/p24 family/GOLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 429
AciI CCGC 3 cut(s) 129, 183, 634
AcsI RAATTY 1 cut(s) 414
AcvI CACGTG 1 cut(s) 193
AfaI GTAC 2 cut(s) 305, 534
AfiI CCNNNNNNNGG 1 cut(s) 205
AflIII ACRYGT 2 cut(s) 190, 192
AgsI TTSAA 2 cut(s) 40, 122
AjiI CACGTC 1 cut(s) 325
AjnI CCWGG 3 cut(s) 51, 356, 400
Alw26I GTCTC 3 cut(s) 306, 331, 507
AoxI GGCC 1 cut(s) 584
ApeKI GCWGC 1 cut(s) 539
ApoI RAATTY 1 cut(s) 414
Asp700I GAANNNNTTC 1 cut(s) 414
AspS9I GGNCC 2 cut(s) 55, 398
AsuHPI GGTGA 2 cut(s) 470, 590
AvaII GGWCC 2 cut(s) 55, 398
BauI CACGAG 1 cut(s) 386
BbrPI CACGTG 1 cut(s) 193
BbsI GAAGAC 1 cut(s) 615
BbvI GCAGC 1 cut(s) 526
BccI CCATC 2 cut(s) 163, 595
BceAI ACGGC 1 cut(s) 391
BciT130I CCWGG 3 cut(s) 53, 358, 402
BclI TGATCA 1 cut(s) 643
BcoDI GTCTC 3 cut(s) 306, 331, 507
BfaI CTAG 1 cut(s) 144
BisI GCNGC 1 cut(s) 540
BlsI GCNGC 1 cut(s) 541
Bme1390I CCNGG 3 cut(s) 53, 358, 402
Bme18I GGWCC 2 cut(s) 55, 398
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 2 cut(s) 55, 398
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 3 cut(s) 53, 358, 402
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 1 cut(s) 286
BmuI ACTGGG 1 cut(s) 88
BoxI GACNNNNGTC 1 cut(s) 193
BpiI GAAGAC 1 cut(s) 615
BsaAI YACGTR 1 cut(s) 193
BsaJI CCNNGG 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse1I ACTGG 3 cut(s) 94, 235, 481
BseBI CCWGG 3 cut(s) 53, 358, 402
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 2 cut(s) 99, 622
BseLI CCNNNNNNNGG 1 cut(s) 205
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 3 cut(s) 94, 235, 481
BseXI GCAGC 1 cut(s) 526
Bsh1236I CGCG 1 cut(s) 429
BshFI GGCC 1 cut(s) 586
BslFI GGGAC 2 cut(s) 181, 411
BslI CCNNNNNNNGG 1 cut(s) 205
BsmAI GTCTC 3 cut(s) 306, 331, 507
BsmBI CGTCTC 1 cut(s) 331
BsmFI GGGAC 2 cut(s) 181, 411
BsnI GGCC 1 cut(s) 586
Bsp1407I TGTACA 1 cut(s) 532
Bsp143I GATC 2 cut(s) 382, 643
BspACI CCGC 3 cut(s) 129, 183, 634
BspANI GGCC 1 cut(s) 586
BspCNI CTCAG 1 cut(s) 192
BspFNI CGCG 1 cut(s) 429
BspHI TCATGA 2 cut(s) 292, 418
BspLI GGNNCC 1 cut(s) 399
BsrGI TGTACA 1 cut(s) 532
BsrI ACTGG 3 cut(s) 94, 235, 481
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 2 cut(s) 382, 643
BssSI CACGAG 1 cut(s) 386
Bst2BI CACGAG 1 cut(s) 386
Bst2UI CCWGG 3 cut(s) 53, 358, 402
Bst4CI ACNGT 1 cut(s) 578
Bst6I CTCTTC 1 cut(s) 273
BstAUI TGTACA 1 cut(s) 532
BstBAI YACGTR 1 cut(s) 193
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 1 cut(s) 200
BstF5I GGATG 2 cut(s) 99, 622
BstFNI CGCG 1 cut(s) 429
BstKTI GATC 2 cut(s) 385, 646
BstMAI GTCTC 3 cut(s) 306, 331, 507
BstMBI GATC 2 cut(s) 382, 643
BstNI CCWGG 3 cut(s) 53, 358, 402
BstNSI RCATGY 2 cut(s) 539, 569
BstPAI GACNNNNGTC 1 cut(s) 193
BstSCI CCNGG 3 cut(s) 51, 356, 400
BstUI CGCG 1 cut(s) 429
BstV1I GCAGC 1 cut(s) 526
BstV2I GAAGAC 1 cut(s) 615
BsuRI GGCC 1 cut(s) 586
BtrI CACGTC 1 cut(s) 325
BtsCI GGATG 2 cut(s) 99, 622
BtsIMutI CAGTG 1 cut(s) 646
Cac8I GCNNGC 1 cut(s) 567
CciI TCATGA 2 cut(s) 292, 418
Cfr13I GGNCC 2 cut(s) 55, 398
CsiI ACCWGGT 1 cut(s) 400
Csp6I GTAC 2 cut(s) 304, 533
CviAII CATG 6 cut(s) 293, 307, 419, 536, 557, 566
CviJI RGCY 2 cut(s) 64, 586
CviKI_1 RGCY 2 cut(s) 64, 586
CviQI GTAC 2 cut(s) 304, 533
DdeI CTNAG 1 cut(s) 200
DpnI GATC 2 cut(s) 384, 645
DpnII GATC 2 cut(s) 382, 643
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
EciI GGCGGA 3 cut(s) 118, 198, 649
Eco47I GGWCC 2 cut(s) 55, 398
Eco72I CACGTG 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 398
EcoRII CCWGG 3 cut(s) 51, 356, 400
Esp3I CGTCTC 1 cut(s) 331
FaeI CATG 6 cut(s) 296, 310, 422, 539, 560, 569
FaqI GGGAC 2 cut(s) 181, 411
FatI CATG 6 cut(s) 292, 306, 418, 535, 556, 565
FbaI TGATCA 1 cut(s) 643
Fnu4HI GCNGC 1 cut(s) 540
FokI GGATG 2 cut(s) 86, 629
Fsp4HI GCNGC 1 cut(s) 540
FspBI CTAG 1 cut(s) 144
GluI GCNGC 1 cut(s) 540
HaeIII GGCC 1 cut(s) 586
Hin1II CATG 6 cut(s) 296, 310, 422, 539, 560, 569
HinfI GANTC 3 cut(s) 319, 350, 620
HphI GGTGA 2 cut(s) 470, 590
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 3 cut(s) 59, 158, 465
Hpy188III TCNNGA 4 cut(s) 265, 293, 388, 419
Hpy8I GTNNAC 1 cut(s) 478
Hpy99I CGWCG 2 cut(s) 374, 377
HpyAV CCTTC 1 cut(s) 236
HpyCH4III ACNGT 1 cut(s) 578
HpyCH4IV ACGT 2 cut(s) 192, 324
HpyCH4V TGCA 1 cut(s) 299
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 2 cut(s) 192, 324
Hsp92II CATG 6 cut(s) 296, 310, 422, 539, 560, 569
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 2 cut(s) 382, 643
Lsp1109I GCAGC 1 cut(s) 526
LweI GCATC 1 cut(s) 286
MabI ACCWGGT 1 cut(s) 400
MaeI CTAG 1 cut(s) 144
MaeII ACGT 2 cut(s) 192, 324
MalI GATC 2 cut(s) 384, 645
MboI GATC 2 cut(s) 382, 643
MboII GAAGA 3 cut(s) 178, 290, 615
MluCI AATT 1 cut(s) 414
MlyI GAGTC 1 cut(s) 629
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 6 cut(s) 136, 152, 156, 179, 252, 576
MroXI GAANNNNTTC 1 cut(s) 414
MspR9I CCNGG 3 cut(s) 53, 358, 402
MvaI CCWGG 3 cut(s) 53, 358, 402
MvnI CGCG 1 cut(s) 429
NdeII GATC 2 cut(s) 382, 643
NlaIII CATG 6 cut(s) 296, 310, 422, 539, 560, 569
NlaIV GGNNCC 1 cut(s) 399
NmeAIII GCCGAG 1 cut(s) 90
NspI RCATGY 2 cut(s) 539, 569
PaeI GCATGC 1 cut(s) 569
PagI TCATGA 2 cut(s) 292, 418
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PdmI GAANNNNTTC 1 cut(s) 414
PfeI GAWTC 2 cut(s) 319, 350
PkrI GCNGC 1 cut(s) 541
PleI GAGTC 1 cut(s) 628
PmaCI CACGTG 1 cut(s) 193
PmlI CACGTG 1 cut(s) 193
PpsI GAGTC 1 cut(s) 628
Ppu21I YACGTR 1 cut(s) 193
PpuMI RGGWCCY 1 cut(s) 398
PshAI GACNNNNGTC 1 cut(s) 193
Psp5II RGGWCCY 1 cut(s) 398
Psp6I CCWGG 3 cut(s) 51, 356, 400
PspCI CACGTG 1 cut(s) 193
PspGI CCWGG 3 cut(s) 51, 356, 400
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 2 cut(s) 55, 398
PspPPI RGGWCCY 1 cut(s) 398
RsaI GTAC 2 cut(s) 305, 534
RsaNI GTAC 2 cut(s) 304, 533
SatI GCNGC 1 cut(s) 540
Sau3AI GATC 2 cut(s) 382, 643
Sau96I GGNCC 2 cut(s) 55, 398
SchI GAGTC 1 cut(s) 629
ScrFI CCNGG 3 cut(s) 53, 358, 402
SetI ASST 9 cut(s) 128, 152, 195, 263, 327, 359, 403, 456, 475
SexAI ACCWGGT 1 cut(s) 400
SfaNI GCATC 1 cut(s) 286
SinI GGWCC 2 cut(s) 55, 398
SphI GCATGC 1 cut(s) 569
Sse9I AATT 1 cut(s) 414
SsiI CCGC 3 cut(s) 129, 183, 634
SspMI CTAG 1 cut(s) 144
StyD4I CCNGG 3 cut(s) 51, 356, 400
TaaI ACNGT 1 cut(s) 578
TaiI ACGT 2 cut(s) 195, 327
TaqI TCGA 3 cut(s) 71, 348, 353
TasI AATT 1 cut(s) 414
TatI WGTACW 2 cut(s) 303, 532
TfiI GAWTC 2 cut(s) 319, 350
TseI GCWGC 1 cut(s) 539
TspDTI ATGAA 2 cut(s) 88, 407
TspGWI ACGGA 1 cut(s) 598
VpaK11BI GGWCC 2 cut(s) 55, 398
XapI RAATTY 1 cut(s) 414
XceI RCATGY 2 cut(s) 539, 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XmnI GAANNNNTTC 1 cut(s) 414
XspI CTAG 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.