pycom17g15720

membrane protein At1g16860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001653
Physical Location & Seq
Forward (+)
13734049 .. 13737561
3513 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1449 bp
ATGGGTTCCAGATTCCCATCTCATCAGCTCAGCAATGGTCTCTATGTATCAGGTAGGCCTGAGCAGCCAAAAGAAAGGGCCCCTACGATGGGCTCAACTGCTATGCCCTATACGGGTGGTGACATCAAGAAGTCAGGAGAGCTGGGGAAAATGTTTGACATCCCTATGGATGGGTCCAAGTCTAGGAAATCCGGGCAATTGACCAGTGCTCCTTCAAGGACCGGATCATTTGGAGGTGCGGCTTCACATTCAGGTCCTATCATGCCTAATGCTGCAGCTCGGGCAAGCTATACAACATCAGGTCCTGTATCATCTGTAGGCATGACAGGTTCTGTTTCAGTAAAGAAGACTAATTCCGGGCCACTCAACAAGCATGGTGAACCATTGAAGAAGTCTTCTGGCCCTCAATCTGGTGGAGTGACACGTCAAAACTCTGGCCATATTCCACCTGTTCTTCCCACAACAGGACTTATCACATCTGGTCCCATTTCTTCAGGTCCTCTAAATTCCTCAGGAGCCCCTAGAAAGGTATCTGGACCTTTAGAGTCTAAGGGATCAATGAAATTACAAGGTTCCTCAGTTACTCACAATCCAGCAGTGACTACTCTTGGCCAAGATGAGTTTTCTTTCGGGAGGAACTTCCCAAAGACAATATTATGGTCTGTGGTACTGATATTTGTGATGGGGTTCATAGCTGGTTGTTTTATTCTTGGAGCAGTCCACAATGCCATACTCCTTGCTGTTGTGGGAATTCTTTTCGCTGGAGTTGCTGCTTTGTTCACATGGAATTCTTGTTGGGGAAGAAACGCTATTATAAATTTCATTTCTCGGTATCCTGATGCTGAGCTCAGAACTGCAAAGAATGGGCAGTACATGAAAGTCTCTGGGGTGGTTACCTGTGGTAACGTTCCCCTTGAGTCATCCTTCCGCAGAGTTCCCAGATGTGTTTACACATCTACGAGTTTATATGAGTACCGAGGATGGGGTTCCAAACCAGCAAACCCTACACATCGTCGGTTTACATGGGGACTTAGATCATCGGAGAGGCATGTAGCAGACTTCTACATCTCTGATTTCCAGTCTGGTTTGAGAGCATTAGTTAAGACCGGCTATGGAGCAAGAGTTACTCCTTACATTGATGAGTCCGTCATTATTGACGTTAACCCAGAGAACAAAGACATGTCTCCTGACTTTCTCAGGTGGTTGGCAGAGCGGAATCTTTCAAGTGATGACCGTACAATGCGATTGAAAGAAGGGTACATCAAGGAAGGTAGCACGGTAAGTGTGATGGGAGTTGTCCAGAGAAATGACAATGTGCTTATGATCGTCCCTCCACCTGAGCCATTGACAACTGGATGCCAGTGGAGCAATTGTATTTTTCCAGCTAGCCTTGAGGGGGTGGTTTTGAGATGCGAAGACTCCTCGAAAAACGATGTAATACCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

483

Amino Acids

51.65

Weight (kDa)

9.62

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 815
AccBSI CCGCTC 1 cut(s) 1213
AciI CCGC 3 cut(s) 239, 928, 1213
AclI AACGTT 1 cut(s) 906
AclWI GGATC 2 cut(s) 232, 562
AcoI YGGCCR 2 cut(s) 436, 610
AcsI RAATTY 4 cut(s) 505, 750, 787, 817
AcuI CTGAAG 1 cut(s) 477
AfaI GTAC 5 cut(s) 669, 872, 974, 1237, 1259
AflIII ACRYGT 2 cut(s) 422, 1179
AgsI TTSAA 4 cut(s) 216, 388, 1224, 1249
AjiI CACGTC 1 cut(s) 425
AluBI AGCT 7 cut(s) 28, 142, 278, 288, 695, 847, 1385
AluI AGCT 7 cut(s) 28, 142, 278, 288, 695, 847, 1385
Alw21I GWGCWC 2 cut(s) 211, 849
Alw26I GTCTC 3 cut(s) 44, 886, 1188
AlwI GGATC 2 cut(s) 232, 562
AlwNI CAGNNNCTG 2 cut(s) 305, 332
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 6 cut(s) 56, 78, 359, 400, 436, 610
ApaI GGGCCC 1 cut(s) 82
ApeKI GCWGC 4 cut(s) 64, 272, 275, 770
ApoI RAATTY 4 cut(s) 505, 750, 787, 817
AsuC2I CCSGG 2 cut(s) 193, 358
AsuHPI GGTGA 2 cut(s) 131, 389
AsuNHI GCTAGC 1 cut(s) 1385
AvaI CYCGRG 1 cut(s) 279
AvaII GGWCC 7 cut(s) 174, 219, 254, 302, 482, 497, 536
AxyI CCTNAGG 1 cut(s) 511
BaeGI GKGCMC 1 cut(s) 82
BalI TGGCCA 2 cut(s) 438, 612
BanII GRGCYC 4 cut(s) 82, 95, 520, 849
BbsI GAAGAC 3 cut(s) 353, 387, 1422
Bbv12I GWGCWC 2 cut(s) 211, 849
BbvI GCAGC 4 cut(s) 76, 259, 287, 757
BccI CCATC 6 cut(s) 25, 82, 164, 676, 975, 1282
BciVI GTATCC 1 cut(s) 843
BcnI CCSGG 2 cut(s) 193, 358
BcoDI GTCTC 3 cut(s) 44, 886, 1188
BfaI CTAG 3 cut(s) 183, 522, 1386
BfmI CTRYAG 2 cut(s) 273, 315
BfuI GTATCC 1 cut(s) 843
BisI GCNGC 5 cut(s) 65, 240, 273, 276, 771
BlpI GCTNAGC 2 cut(s) 29, 843
BlsI GCNGC 5 cut(s) 66, 241, 274, 277, 772
Bme1390I CCNGG 2 cut(s) 193, 358
Bme18I GGWCC 7 cut(s) 174, 219, 254, 302, 482, 497, 536
BmeT110I CYCGRG 1 cut(s) 279
BmgBI CACGTC 1 cut(s) 425
BmiI GGNNCC 8 cut(s) 7, 80, 81, 175, 484, 517, 574, 988
BmrFI CCNGG 2 cut(s) 193, 358
BmsI GCATC 3 cut(s) 829, 1346, 1400
BmtI GCTAGC 1 cut(s) 1389
BpiI GAAGAC 3 cut(s) 353, 387, 1422
BpmI CTGGAG 1 cut(s) 783
Bpu10I CCTNAGC 2 cut(s) 60, 1338
Bpu1102I GCTNAGC 2 cut(s) 29, 843
BpuEI CTTGAG 2 cut(s) 935, 1412
BpuMI CCSGG 2 cut(s) 193, 358
BsaBI GATNNNNATC 1 cut(s) 16
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 976
BsaWI WCCGGW 1 cut(s) 221
BsaXI ACNNNNNCTCC 6 cut(s) 193, 223, 1107, 1137, 1357, 1387
Bse118I RCCGGY 1 cut(s) 1106
Bse1I ACTGG 5 cut(s) 204, 1078, 1357, 1360, 1442
Bse21I CCTNAGG 1 cut(s) 511
Bse3DI GCAATG 1 cut(s) 40
Bse8I GATNNNNATC 1 cut(s) 16
BseDI CCNNGG 1 cut(s) 976
BseGI GGATG 5 cut(s) 159, 175, 920, 986, 1361
BseJI GATNNNNATC 1 cut(s) 16
BseMI GCAATG 1 cut(s) 40
BseMII CTCAG 8 cut(s) 43, 51, 525, 591, 834, 862, 1210, 1329
BseNI ACTGG 5 cut(s) 204, 1078, 1357, 1360, 1442
BseRI GAGGAG 1 cut(s) 1411
BseSI GKGCMC 1 cut(s) 82
BseXI GCAGC 4 cut(s) 76, 259, 287, 757
BseYI CCCAGC 1 cut(s) 142
BshFI GGCC 6 cut(s) 58, 80, 361, 402, 438, 612
BsiHKAI GWGCWC 2 cut(s) 211, 849
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 4 cut(s) 192, 222, 357, 1107
BslFI GGGAC 3 cut(s) 468, 1041, 1313
BsmAI GTCTC 3 cut(s) 44, 886, 1188
BsmFI GGGAC 3 cut(s) 468, 1041, 1313
BsnI GGCC 6 cut(s) 58, 80, 361, 402, 438, 612
Bso31I GGTCTC 1 cut(s) 44
BsoBI CYCGRG 1 cut(s) 279
Bsp120I GGGCCC 1 cut(s) 78
Bsp1286I GDGCHC 5 cut(s) 82, 95, 211, 520, 849
Bsp143I GATC 4 cut(s) 224, 554, 1034, 1323
Bsp1720I GCTNAGC 2 cut(s) 29, 843
BspACI CCGC 3 cut(s) 239, 928, 1213
BspANI GGCC 6 cut(s) 58, 80, 361, 402, 438, 612
BspCNI CTCAG 8 cut(s) 42, 52, 524, 590, 835, 861, 1209, 1330
BspLI GGNNCC 8 cut(s) 7, 80, 81, 175, 484, 517, 574, 988
BspMAI CTGCAG 1 cut(s) 277
BspOI GCTAGC 1 cut(s) 1389
BspPI GGATC 2 cut(s) 232, 562
BspTNI GGTCTC 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 1213
BsrDI GCAATG 1 cut(s) 40
BsrFI RCCGGY 1 cut(s) 1106
BsrI ACTGG 5 cut(s) 204, 1078, 1357, 1360, 1442
BssAI RCCGGY 1 cut(s) 1106
BssECI CCNNGG 1 cut(s) 976
BssMI GATC 4 cut(s) 224, 554, 1034, 1323
Bst4CI ACNGT 2 cut(s) 1235, 1279
BstC8I GCNNGC 2 cut(s) 286, 1387
BstEII GGTNACC 1 cut(s) 892
BstF5I GGATG 5 cut(s) 159, 175, 920, 986, 1361
BstKTI GATC 4 cut(s) 227, 557, 1037, 1326
BstMAI GTCTC 3 cut(s) 44, 886, 1188
BstMBI GATC 4 cut(s) 224, 554, 1034, 1323
BstMWI GCNNNNNNNGC 4 cut(s) 64, 281, 767, 1365
BstNSI RCATGY 2 cut(s) 1052, 1183
BstPI GGTNACC 1 cut(s) 892
BstSCI CCNGG 2 cut(s) 191, 356
BstSFI CTRYAG 2 cut(s) 273, 315
BstSLI GKGCMC 1 cut(s) 82
BstV1I GCAGC 4 cut(s) 76, 259, 287, 757
BstV2I GAAGAC 3 cut(s) 353, 387, 1422
Bsu36I CCTNAGG 1 cut(s) 511
BsuI GTATCC 1 cut(s) 843
BsuRI GGCC 6 cut(s) 58, 80, 361, 402, 438, 612
BtrI CACGTC 1 cut(s) 425
BtsCI GGATG 5 cut(s) 159, 175, 920, 986, 1361
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 3 cut(s) 211, 603, 1367
Cac8I GCNNGC 2 cut(s) 286, 1387
CaiI CAGNNNCTG 2 cut(s) 305, 332
Cfr10I RCCGGY 1 cut(s) 1106
Csp6I GTAC 5 cut(s) 668, 871, 973, 1236, 1258
CviAII CATG 8 cut(s) 262, 322, 374, 783, 874, 1023, 1049, 1180
CviQI GTAC 5 cut(s) 668, 871, 973, 1236, 1258
DpnI GATC 4 cut(s) 226, 556, 1036, 1325
DpnII GATC 4 cut(s) 224, 554, 1034, 1323
EaeI YGGCCR 2 cut(s) 436, 610
Ecl136II GAGCTC 1 cut(s) 847
Eco147I AGGCCT 1 cut(s) 58
Eco24I GRGCYC 4 cut(s) 82, 95, 520, 849
Eco31I GGTCTC 1 cut(s) 44
Eco47I GGWCC 7 cut(s) 174, 219, 254, 302, 482, 497, 536
Eco53kI GAGCTC 1 cut(s) 847
Eco57I CTGAAG 1 cut(s) 477
Eco81I CCTNAGG 1 cut(s) 511
Eco88I CYCGRG 1 cut(s) 279
Eco91I GGTNACC 1 cut(s) 892
EcoICRI GAGCTC 1 cut(s) 847
EcoO109I RGGNCCY 5 cut(s) 78, 79, 254, 302, 497
EcoO65I GGTNACC 1 cut(s) 892
EcoRI GAATTC 2 cut(s) 750, 787
EcoT38I GRGCYC 4 cut(s) 82, 95, 520, 849
FaeI CATG 8 cut(s) 265, 325, 377, 786, 877, 1026, 1052, 1183
FaqI GGGAC 3 cut(s) 468, 1041, 1313
FatI CATG 8 cut(s) 261, 321, 373, 782, 873, 1022, 1048, 1179
Fnu4HI GCNGC 5 cut(s) 65, 240, 273, 276, 771
FokI GGATG 5 cut(s) 146, 182, 907, 993, 1368
FriOI GRGCYC 4 cut(s) 82, 95, 520, 849
Fsp4HI GCNGC 5 cut(s) 65, 240, 273, 276, 771
FspBI CTAG 3 cut(s) 183, 522, 1386
GluI GCNGC 5 cut(s) 65, 240, 273, 276, 771
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 6 cut(s) 58, 80, 361, 402, 438, 612
HapII CCGG 4 cut(s) 192, 222, 357, 1107
Hin1II CATG 8 cut(s) 265, 325, 377, 786, 877, 1026, 1052, 1183
HincII GTYRAC 1 cut(s) 1162
HindII GTYRAC 1 cut(s) 1162
HinfI GANTC 6 cut(s) 12, 545, 917, 1142, 1216, 1418
HpaI GTTAAC 1 cut(s) 1162
HpaII CCGG 4 cut(s) 192, 222, 357, 1107
HphI GGTGA 2 cut(s) 131, 389
Hpy166II GTNNAC 6 cut(s) 380, 721, 780, 949, 1020, 1162
Hpy188I TCNGA 3 cut(s) 851, 1042, 1072
Hpy188III TCNNGA 9 cut(s) 9, 127, 135, 513, 534, 631, 836, 1187, 1300
Hpy8I GTNNAC 6 cut(s) 380, 721, 780, 949, 1020, 1162
Hpy99I CGWCG 1 cut(s) 1017
HpyAV CCTTC 4 cut(s) 222, 934, 1247, 1262
HpyCH4III ACNGT 2 cut(s) 1235, 1279
HpyCH4IV ACGT 3 cut(s) 424, 906, 1158
HpyCH4V TGCA 2 cut(s) 275, 857
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 281, 767, 1365
HpySE526I ACGT 3 cut(s) 424, 906, 1158
Hsp92II CATG 8 cut(s) 265, 325, 377, 786, 877, 1026, 1052, 1183
KspAI GTTAAC 1 cut(s) 1162
Kzo9I GATC 4 cut(s) 224, 554, 1034, 1323
LmnI GCTCC 5 cut(s) 214, 515, 713, 1115, 1365
Lsp1109I GCAGC 4 cut(s) 76, 259, 287, 757
LweI GCATC 3 cut(s) 829, 1346, 1400
MaeI CTAG 3 cut(s) 183, 522, 1386
MaeII ACGT 3 cut(s) 424, 906, 1158
MaeIII GTNAC 7 cut(s) 119, 418, 580, 598, 892, 902, 1123
MalI GATC 4 cut(s) 226, 556, 1036, 1325
MbiI CCGCTC 1 cut(s) 1213
MboI GATC 4 cut(s) 224, 554, 1034, 1323
MboII GAAGA 7 cut(s) 358, 387, 400, 446, 483, 813, 1427
MfeI CAATTG 2 cut(s) 197, 1369
MhlI GDGCHC 5 cut(s) 82, 95, 211, 520, 849
MlsI TGGCCA 2 cut(s) 438, 612
MluCI AATT 8 cut(s) 197, 352, 505, 563, 750, 787, 817, 1369
MluNI TGGCCA 2 cut(s) 438, 612
MlyI GAGTC 4 cut(s) 554, 926, 1151, 1412
Mox20I TGGCCA 2 cut(s) 438, 612
MscI TGGCCA 2 cut(s) 438, 612
MseI TTAA 2 cut(s) 1101, 1161
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 2 cut(s) 438, 612
MspI CCGG 4 cut(s) 192, 222, 357, 1107
MspR9I CCNGG 2 cut(s) 193, 358
MunI CAATTG 2 cut(s) 197, 1369
MwoI GCNNNNNNNGC 4 cut(s) 64, 281, 767, 1365
NciI CCSGG 2 cut(s) 193, 358
NdeII GATC 4 cut(s) 224, 554, 1034, 1323
NheI GCTAGC 1 cut(s) 1385
NlaIII CATG 8 cut(s) 265, 325, 377, 786, 877, 1026, 1052, 1183
NlaIV GGNNCC 8 cut(s) 7, 80, 81, 175, 484, 517, 574, 988
NmuCI GTSAC 3 cut(s) 119, 418, 598
NspI RCATGY 2 cut(s) 1052, 1183
PceI AGGCCT 1 cut(s) 58
PciI ACATGT 1 cut(s) 1179
PfeI GAWTC 2 cut(s) 12, 1216
PkrI GCNGC 5 cut(s) 66, 241, 274, 277, 772
PleI GAGTC 4 cut(s) 553, 925, 1150, 1412
PpsI GAGTC 4 cut(s) 553, 925, 1150, 1412
PpuMI RGGWCCY 3 cut(s) 254, 302, 497
PscI ACATGT 1 cut(s) 1179
PsiI TTATAA 1 cut(s) 815
Psp124BI GAGCTC 1 cut(s) 849
Psp1406I AACGTT 1 cut(s) 906
Psp5II RGGWCCY 3 cut(s) 254, 302, 497
PspEI GGTNACC 1 cut(s) 892
PspFI CCCAGC 1 cut(s) 142
PspN4I GGNNCC 8 cut(s) 7, 80, 81, 175, 484, 517, 574, 988
PspOMI GGGCCC 1 cut(s) 78
PspPPI RGGWCCY 3 cut(s) 254, 302, 497
PstI CTGCAG 1 cut(s) 277
PstNI CAGNNNCTG 2 cut(s) 305, 332
RsaI GTAC 5 cut(s) 669, 872, 974, 1237, 1259
RsaNI GTAC 5 cut(s) 668, 871, 973, 1236, 1258
RseI CAYNNNNRTG 1 cut(s) 164
SacI GAGCTC 1 cut(s) 849
SaqAI TTAA 2 cut(s) 1101, 1161
SatI GCNGC 5 cut(s) 65, 240, 273, 276, 771
Sau3AI GATC 4 cut(s) 224, 554, 1034, 1323
SchI GAGTC 4 cut(s) 554, 926, 1151, 1412
ScrFI CCNGG 2 cut(s) 193, 358
SduI GDGCHC 5 cut(s) 82, 95, 211, 520, 849
SfaNI GCATC 3 cut(s) 829, 1346, 1400
SfcI CTRYAG 2 cut(s) 273, 315
SinI GGWCC 7 cut(s) 174, 219, 254, 302, 482, 497, 536
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 2 cut(s) 914, 1391
SmoI CTYRAG 2 cut(s) 914, 1391
Sse9I AATT 8 cut(s) 197, 352, 505, 563, 750, 787, 817, 1369
SseBI AGGCCT 1 cut(s) 58
SsiI CCGC 3 cut(s) 239, 928, 1213
SspI AATATT 1 cut(s) 654
SspMI CTAG 3 cut(s) 183, 522, 1386
SstI GAGCTC 1 cut(s) 849
StuI AGGCCT 1 cut(s) 58
StyD4I CCNGG 2 cut(s) 191, 356
TaaI ACNGT 2 cut(s) 1235, 1279
TaiI ACGT 3 cut(s) 427, 909, 1161
TaqI TCGA 1 cut(s) 1424
TasI AATT 8 cut(s) 197, 352, 505, 563, 750, 787, 817, 1369
TatI WGTACW 1 cut(s) 870
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 2 cut(s) 12, 1216
Tru1I TTAA 2 cut(s) 1101, 1161
Tru9I TTAA 2 cut(s) 1101, 1161
TscAI CASTG 3 cut(s) 211, 603, 1367
TseFI GTSAC 3 cut(s) 119, 418, 598
TseI GCWGC 4 cut(s) 64, 272, 275, 770
Tsp45I GTSAC 3 cut(s) 119, 418, 598
TspDTI ATGAA 4 cut(s) 575, 679, 811, 890
TspGWI ACGGA 1 cut(s) 1135
TspRI CASTG 3 cut(s) 211, 603, 1367
VpaK11BI GGWCC 7 cut(s) 174, 219, 254, 302, 482, 497, 536
XapI RAATTY 4 cut(s) 505, 750, 787, 817
XceI RCATGY 2 cut(s) 1052, 1183
XspI CTAG 3 cut(s) 183, 522, 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.