pycom17g19120

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003411
Physical Location & Seq
Forward (+)
16754909 .. 16756030
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
ATGGGTGCAGGTAGTAAAGAACAAATGCTTCGAGGTCCATGCTCGATCACAAAATATGACAACTACACAAAAAGGGAAGATAGCTGGGTAATCATGGATGGGGATCATGATGATATGTACGACACAACATCCTCGACGTCGATTTCGAACAGATCATCGACATCTTCATCGGACTTAGTAGATGATGCATCTTCATCATCAATGTGGTCGTCATGTTCTTCTTCCCTTCATTCTCATGGATCGTTGTTCGACTTGTCAGATCTCATGGATCAGCTACCCATCAAGAGAGGACTTTCAAAGTATTTTGAAGGCAAGTGCCAATCCTTTACATCTTTATCAAAGGCCAAGAGCATTGAAGATCTTGCAAAGAAAGAGTCTCCATATAAACAAAGAAAGGCAATAAAAGCAAGCAAGAGCTATGGCGGTGGCTTGAGTTCTTACAGATCGTACACACTTCCGAAGGCCACCATTTCTAAGAGTAAGAAGCCATCAACTACTACATCTTTTTCCAGCAGATCTAGAGGCAGCTCTTTCGGCATAAATAACAATATGCTCCCTCGAATTCCAGTAAGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

21.1

Weight (kDa)

9.52

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 140
AciI CCGC 1 cut(s) 423
AclWI GGATC 3 cut(s) 111, 247, 276
AcsI RAATTY 1 cut(s) 561
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 2 cut(s) 119, 449
AgsI TTSAA 3 cut(s) 297, 308, 356
AluBI AGCT 4 cut(s) 84, 274, 417, 528
AluI AGCT 4 cut(s) 84, 274, 417, 528
Alw26I GTCTC 1 cut(s) 381
AlwI GGATC 3 cut(s) 111, 247, 276
AoxI GGCC 2 cut(s) 342, 462
ApeKI GCWGC 1 cut(s) 525
ApoI RAATTY 1 cut(s) 561
ArsI GACNNNNNNTTYG 4 cut(s) 127, 159, 359, 391
AspS9I GGNCC 1 cut(s) 35
AsuII TTCGAA 1 cut(s) 146
AvaII GGWCC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 537
BccI CCATC 3 cut(s) 92, 287, 496
BcgI CGANNNNNNTGC 4 cut(s) 21, 55, 514, 548
BcoDI GTCTC 1 cut(s) 381
BfaI CTAG 1 cut(s) 519
BglII AGATCT 3 cut(s) 259, 358, 515
BisI GCNGC 1 cut(s) 526
BlsI GCNGC 1 cut(s) 527
Bme18I GGWCC 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 35
BmsI GCATC 2 cut(s) 175, 197
Bpu14I TTCGAA 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 451
BsaBI GATNNNNATC 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 566
Bse8I GATNNNNATC 1 cut(s) 102
BseGI GGATG 2 cut(s) 103, 128
BseJI GATNNNNATC 1 cut(s) 102
BseNI ACTGG 1 cut(s) 566
BseXI GCAGC 1 cut(s) 537
BseYI CCCAGC 1 cut(s) 84
BsgI GTGCAG 1 cut(s) 27
BshFI GGCC 2 cut(s) 344, 464
BsmAI GTCTC 1 cut(s) 381
BsnI GGCC 2 cut(s) 344, 464
Bsp119I TTCGAA 1 cut(s) 146
Bsp143I GATC 9 cut(s) 45, 103, 152, 239, 259, 268, 358, 443, 515
BspACI CCGC 1 cut(s) 423
BspANI GGCC 2 cut(s) 344, 464
BspHI TCATGA 1 cut(s) 106
BspPI GGATC 3 cut(s) 111, 247, 276
BspT104I TTCGAA 1 cut(s) 146
BsrI ACTGG 1 cut(s) 566
BssMI GATC 9 cut(s) 45, 103, 152, 239, 259, 268, 358, 443, 515
BssNI GRCGYC 1 cut(s) 137
BstACI GRCGYC 1 cut(s) 137
BstBI TTCGAA 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 2 cut(s) 175, 474
BstF5I GGATG 2 cut(s) 103, 128
BstKTI GATC 9 cut(s) 48, 106, 155, 242, 262, 271, 361, 446, 518
BstMAI GTCTC 1 cut(s) 381
BstMBI GATC 9 cut(s) 45, 103, 152, 239, 259, 268, 358, 443, 515
BstMWI GCNNNNNNNGC 2 cut(s) 404, 534
BstV1I GCAGC 1 cut(s) 537
BstX2I RGATCY 3 cut(s) 259, 358, 515
BstYI RGATCY 3 cut(s) 259, 358, 515
BsuRI GGCC 2 cut(s) 344, 464
BtsCI GGATG 2 cut(s) 103, 128
Cac8I GCNNGC 1 cut(s) 409
CciI TCATGA 1 cut(s) 106
Cfr13I GGNCC 1 cut(s) 35
Csp6I GTAC 2 cut(s) 118, 448
CviAII CATG 6 cut(s) 39, 94, 107, 213, 236, 265
CviJI RGCY 8 cut(s) 84, 274, 344, 417, 429, 464, 487, 528
CviKI_1 RGCY 8 cut(s) 84, 274, 344, 417, 429, 464, 487, 528
CviQI GTAC 2 cut(s) 118, 448
DdeI CTNAG 2 cut(s) 175, 474
DpnI GATC 9 cut(s) 47, 105, 154, 241, 261, 270, 360, 445, 517
DpnII GATC 9 cut(s) 45, 103, 152, 239, 259, 268, 358, 443, 515
Eco47I GGWCC 1 cut(s) 35
EcoRI GAATTC 1 cut(s) 561
EcoT22I ATGCAT 1 cut(s) 190
FaeI CATG 6 cut(s) 42, 97, 110, 216, 239, 268
FatI CATG 6 cut(s) 38, 93, 106, 212, 235, 264
Fnu4HI GCNGC 1 cut(s) 526
FokI GGATG 2 cut(s) 110, 115
Fsp4HI GCNGC 1 cut(s) 526
FspBI CTAG 1 cut(s) 519
GluI GCNGC 1 cut(s) 526
GsaI CCCAGC 1 cut(s) 88
HaeIII GGCC 2 cut(s) 344, 464
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 6 cut(s) 42, 97, 110, 216, 239, 268
HinfI GANTC 1 cut(s) 374
Hpy166II GTNNAC 1 cut(s) 450
Hpy188I TCNGA 3 cut(s) 172, 259, 459
Hpy188III TCNNGA 3 cut(s) 107, 283, 519
Hpy8I GTNNAC 1 cut(s) 450
Hpy99I CGWCG 2 cut(s) 139, 142
HpyAV CCTTC 3 cut(s) 236, 302, 454
HpyCH4IV ACGT 1 cut(s) 137
HpyCH4V TGCA 3 cut(s) 8, 188, 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 534
HpyF3I CTNAG 2 cut(s) 175, 474
HpySE526I ACGT 1 cut(s) 137
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 6 cut(s) 42, 97, 110, 216, 239, 268
Kzo9I GATC 9 cut(s) 45, 103, 152, 239, 259, 268, 358, 443, 515
LmnI GCTCC 1 cut(s) 558
LpnPI CCDG 2 cut(s) 70, 523
Lsp1109I GCAGC 1 cut(s) 537
LweI GCATC 2 cut(s) 175, 197
MaeI CTAG 1 cut(s) 519
MaeII ACGT 1 cut(s) 137
MalI GATC 9 cut(s) 47, 105, 154, 241, 261, 270, 360, 445, 517
MboI GATC 9 cut(s) 45, 103, 152, 239, 259, 268, 358, 443, 515
MboII GAAGA 6 cut(s) 89, 156, 183, 210, 213, 368
MflI RGATCY 3 cut(s) 259, 358, 515
MluCI AATT 1 cut(s) 561
MlyI GAGTC 1 cut(s) 383
MnlI CCTC 5 cut(s) 26, 142, 281, 515, 567
Mph1103I ATGCAT 1 cut(s) 190
MslI CAYNNNNRTG 2 cut(s) 202, 234
MwoI GCNNNNNNNGC 2 cut(s) 404, 534
NdeII GATC 9 cut(s) 45, 103, 152, 239, 259, 268, 358, 443, 515
NlaIII CATG 6 cut(s) 42, 97, 110, 216, 239, 268
NsiI ATGCAT 1 cut(s) 190
NspV TTCGAA 1 cut(s) 146
PagI TCATGA 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PkrI GCNGC 1 cut(s) 527
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PspFI CCCAGC 1 cut(s) 84
PspPI GGNCC 1 cut(s) 35
PsuI RGATCY 3 cut(s) 259, 358, 515
RsaI GTAC 2 cut(s) 119, 449
RsaNI GTAC 2 cut(s) 118, 448
RseI CAYNNNNRTG 2 cut(s) 202, 234
SatI GCNGC 1 cut(s) 526
Sau3AI GATC 9 cut(s) 45, 103, 152, 239, 259, 268, 358, 443, 515
Sau96I GGNCC 1 cut(s) 35
SchI GAGTC 1 cut(s) 383
SetI ASST 7 cut(s) 13, 37, 86, 140, 276, 419, 530
SfaNI GCATC 2 cut(s) 175, 197
SfuI TTCGAA 1 cut(s) 146
SinI GGWCC 1 cut(s) 35
SmiMI CAYNNNNRTG 2 cut(s) 202, 234
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
Sse9I AATT 1 cut(s) 561
SsiI CCGC 1 cut(s) 423
SspMI CTAG 1 cut(s) 519
TaiI ACGT 1 cut(s) 140
TaqI TCGA 8 cut(s) 31, 44, 134, 140, 146, 158, 249, 559
TasI AATT 1 cut(s) 561
TseI GCWGC 1 cut(s) 525
TspDTI ATGAA 3 cut(s) 156, 183, 218
VpaK11BI GGWCC 1 cut(s) 35
XapI RAATTY 1 cut(s) 561
XbaI TCTAGA 1 cut(s) 518
XspI CTAG 1 cut(s) 519
ZraI GACGTC 1 cut(s) 138
Zsp2I ATGCAT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.