pycom17g21830

CUE domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003445
Physical Location & Seq
Forward (+)
20261365 .. 20264257
2893 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 729 bp
ATGTCTGCGGCGGTGTGCGGAAGCAAGAGGTCTTTCTTCGAAGATCAATCGCCGCCCCTCGCACAGAGGCTTCCTCCGTCGCCGGTCCTCACAAAGAGGCAGCGCCGTTCGTCTTCTACCTCTCCCATTCGCTTCGCTGCTCCGTATCTTCTCGAGCAGCTTCGCTCTCTTTTCCCTCACATGGACCCTCAGGTTCTTGAGAGAGCATTGGAAGAATGTGGCAACGATATGGATGCTGCCATCAAGAGGCTGCATGAACTTTGCCTAGGATCTGCGGAGGAAACTTCTGCATCTGCTGAACAAACAGCTGATGCCGTAGCTAACGGTCTATTGTCTAATGATGGAGCTGCAGCCACTACTGAGAATCCATTGGTTCAAAACAACCTTCCTGCAGATGGTGCAGAATGGGTTGAGTTGTTTGTAAGGGAAATGATGAGTGCCAGTAGTGTGGATGATGCTAGAGCCCGTGCTGCTAAAGTTTTAGAGGTTTTAGAAAAATCCATCAGTGAACGTGCTGGTGCTGAGGCAGCCCAAAGTTTTCAGAAGGCGAGTATATCTTTTATTATTCTTTTTCTTGTTCTGCATGAACGTCAGAAAGAATTTGAAGAGAGGAACCAGGAATTACAGCATCTGAAGCAGCTAGTTTCTCAGTATCAGGAGCAGTGTAGAACACTAGAGGCAGAGCAAAGCAACTCCATCCCTGGACGTTTCCACCCAGACATCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

243

Amino Acids

26.83

Weight (kDa)

5.08

Isoelectric Point (pI)

59.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 51 - 84 3.5e-09 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 8, 11, 18, 53, 275
AclWI GGATC 1 cut(s) 277
AcsI RAATTY 1 cut(s) 599
AcuI CTGAAG 1 cut(s) 653
AfiI CCNNNNNNNGG 3 cut(s) 181, 246, 395
AgsI TTSAA 2 cut(s) 377, 605
AjnI CCWGG 2 cut(s) 615, 700
AluBI AGCT 5 cut(s) 160, 308, 320, 347, 640
AluI AGCT 5 cut(s) 160, 308, 320, 347, 640
AlwI GGATC 1 cut(s) 277
AlwNI CAGNNNCTG 1 cut(s) 631
Ama87I CYCGRG 1 cut(s) 152
ApoI RAATTY 1 cut(s) 599
AspA2I CCTAGG 1 cut(s) 265
AspLEI GCGC 1 cut(s) 105
AspS9I GGNCC 2 cut(s) 85, 184
AsuII TTCGAA 1 cut(s) 39
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 2 cut(s) 85, 184
AvrII CCTAGG 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 189
BanII GRGCYC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 105
BbvCI CCTCAGC 1 cut(s) 522
BccI CCATC 5 cut(s) 248, 335, 389, 509, 704
BceAI ACGGC 2 cut(s) 90, 299
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 2 cut(s) 617, 702
BfaI CTAG 4 cut(s) 266, 459, 641, 674
BfmI CTRYAG 2 cut(s) 348, 390
BfoI RGCGCY 1 cut(s) 106
BlnI CCTAGG 1 cut(s) 265
Bme1390I CCNGG 2 cut(s) 617, 702
Bme18I GGWCC 2 cut(s) 85, 184
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 2 cut(s) 85, 184
BmiI GGNNCC 2 cut(s) 186, 614
BmrFI CCNGG 2 cut(s) 617, 702
BmsI GCATC 5 cut(s) 223, 299, 301, 445, 637
BpiI GAAGAC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 58, 90
Bpu10I CCTNAGC 1 cut(s) 522
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 218
BsaJI CCNNGG 2 cut(s) 265, 700
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 246, 395
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 441
Bse21I CCTNAGG 1 cut(s) 189
BseBI CCWGG 2 cut(s) 617, 702
BseDI CCNNGG 2 cut(s) 265, 700
BseGI GGATG 3 cut(s) 238, 457, 696
BseLI CCNNNNNNNGG 3 cut(s) 181, 246, 395
BseMII CTCAG 4 cut(s) 203, 351, 513, 662
BseNI ACTGG 1 cut(s) 441
BsgI GTGCAG 1 cut(s) 420
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 1 cut(s) 83
BslI CCNNNNNNNGG 3 cut(s) 181, 246, 395
BsoBI CYCGRG 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 2 cut(s) 43, 269
BspACI CCGC 5 cut(s) 8, 11, 18, 53, 275
BspCNI CTCAG 4 cut(s) 202, 352, 514, 661
BspLI GGNNCC 2 cut(s) 186, 614
BspMAI CTGCAG 2 cut(s) 352, 394
BspPI GGATC 1 cut(s) 277
BspT104I TTCGAA 1 cut(s) 39
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 1 cut(s) 441
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 265, 700
BssMI GATC 2 cut(s) 43, 269
BssT1I CCWWGG 1 cut(s) 265
Bst2UI CCWGG 2 cut(s) 617, 702
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 1 cut(s) 600
BstAPI GCANNNNNTGC 1 cut(s) 398
BstBI TTCGAA 1 cut(s) 39
BstDEI CTNAG 4 cut(s) 189, 360, 522, 648
BstF5I GGATG 3 cut(s) 238, 457, 696
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 2 cut(s) 46, 272
BstMBI GATC 2 cut(s) 43, 269
BstMWI GCNNNNNNNGC 4 cut(s) 398, 470, 527, 634
BstNI CCWGG 2 cut(s) 617, 702
BstSCI CCNGG 2 cut(s) 615, 700
BstSFI CTRYAG 2 cut(s) 348, 390
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 269
BstXI CCANNNNNNTGG 1 cut(s) 448
BstYI RGATCY 1 cut(s) 269
Bsu36I CCTNAGG 1 cut(s) 189
BtsCI GGATG 3 cut(s) 238, 457, 696
BtsI GCAGTG 1 cut(s) 668
BtsIMutI CAGTG 2 cut(s) 511, 668
CaiI CAGNNNCTG 1 cut(s) 631
CfoI GCGC 1 cut(s) 105
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 2 cut(s) 85, 184
CviAII CATG 3 cut(s) 181, 254, 584
DdeI CTNAG 4 cut(s) 189, 360, 522, 648
DpnI GATC 2 cut(s) 45, 271
DpnII GATC 2 cut(s) 43, 269
Eam1104I CTCTTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 600
Eco130I CCWWGG 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 466
Eco47I GGWCC 2 cut(s) 85, 184
Eco57I CTGAAG 1 cut(s) 653
Eco81I CCTNAGG 1 cut(s) 189
Eco88I CYCGRG 1 cut(s) 152
EcoRII CCWGG 2 cut(s) 615, 700
EcoT14I CCWWGG 1 cut(s) 265
EcoT38I GRGCYC 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 3 cut(s) 184, 257, 587
FaiI YATR 5 cut(s) 182, 230, 255, 554, 585
FatI CATG 3 cut(s) 180, 253, 583
FokI GGATG 3 cut(s) 245, 464, 683
FriOI GRGCYC 1 cut(s) 466
FspBI CTAG 4 cut(s) 266, 459, 641, 674
GlaI GCGC 1 cut(s) 104
HaeII RGCGCY 1 cut(s) 106
HapII CCGG 1 cut(s) 83
HhaI GCGC 1 cut(s) 105
Hin1II CATG 3 cut(s) 184, 257, 587
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HinfI GANTC 1 cut(s) 364
HpaII CCGG 1 cut(s) 83
Hpy166II GTNNAC 1 cut(s) 509
Hpy188I TCNGA 3 cut(s) 543, 594, 633
Hpy188III TCNNGA 4 cut(s) 152, 197, 244, 656
Hpy8I GTNNAC 1 cut(s) 509
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 2 cut(s) 395, 538
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 3 cut(s) 511, 589, 706
HpyCH4V TGCA 6 cut(s) 253, 290, 350, 392, 401, 583
HpyF10VI GCNNNNNNNGC 4 cut(s) 398, 470, 527, 634
HpyF3I CTNAG 4 cut(s) 189, 360, 522, 648
HpySE526I ACGT 3 cut(s) 511, 589, 706
Hsp92II CATG 3 cut(s) 184, 257, 587
HspAI GCGC 1 cut(s) 103
Kzo9I GATC 2 cut(s) 43, 269
LmnI GCTCC 3 cut(s) 145, 344, 658
LweI GCATC 5 cut(s) 223, 299, 301, 445, 637
MaeI CTAG 4 cut(s) 266, 459, 641, 674
MaeII ACGT 3 cut(s) 511, 589, 706
MalI GATC 2 cut(s) 45, 271
MboI GATC 2 cut(s) 43, 269
MboII GAAGA 6 cut(s) 28, 53, 105, 140, 224, 617
MflI RGATCY 1 cut(s) 269
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 2 cut(s) 599, 620
MseI TTAA 1 cut(s) 727
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 2 cut(s) 617, 702
MvaI CCWGG 2 cut(s) 617, 702
MwoI GCNNNNNNNGC 4 cut(s) 398, 470, 527, 634
NdeII GATC 2 cut(s) 43, 269
NlaIII CATG 3 cut(s) 184, 257, 587
NlaIV GGNNCC 2 cut(s) 186, 614
NspV TTCGAA 1 cut(s) 39
PaeR7I CTCGAG 1 cut(s) 152
PfeI GAWTC 1 cut(s) 364
Psp6I CCWGG 2 cut(s) 615, 700
PspGI CCWGG 2 cut(s) 615, 700
PspN4I GGNNCC 2 cut(s) 186, 614
PspPI GGNCC 2 cut(s) 85, 184
PstI CTGCAG 2 cut(s) 352, 394
PstNI CAGNNNCTG 1 cut(s) 631
PsuI RGATCY 1 cut(s) 269
PvuII CAGCTG 1 cut(s) 308
SaqAI TTAA 1 cut(s) 727
Sau3AI GATC 2 cut(s) 43, 269
Sau96I GGNCC 2 cut(s) 85, 184
ScrFI CCNGG 2 cut(s) 617, 702
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 5 cut(s) 223, 299, 301, 445, 637
SfcI CTRYAG 2 cut(s) 348, 390
Sfr274I CTCGAG 1 cut(s) 152
SfuI TTCGAA 1 cut(s) 39
SinI GGWCC 2 cut(s) 85, 184
SlaI CTCGAG 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 152, 197
SmoI CTYRAG 2 cut(s) 152, 197
Sse9I AATT 2 cut(s) 599, 620
SsiI CCGC 5 cut(s) 8, 11, 18, 53, 275
SspMI CTAG 4 cut(s) 266, 459, 641, 674
StyD4I CCNGG 2 cut(s) 615, 700
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 3 cut(s) 514, 592, 709
TaqI TCGA 2 cut(s) 39, 153
TasI AATT 2 cut(s) 599, 620
TauI GCSGC 2 cut(s) 11, 55
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 1 cut(s) 727
Tru9I TTAA 1 cut(s) 727
TscAI CASTG 2 cut(s) 511, 668
TspDTI ATGAA 2 cut(s) 270, 600
TspGWI ACGGA 2 cut(s) 66, 132
TspRI CASTG 2 cut(s) 511, 668
VpaK11BI GGWCC 2 cut(s) 85, 184
XapI RAATTY 1 cut(s) 599
XhoI CTCGAG 1 cut(s) 152
XmaJI CCTAGG 1 cut(s) 265
XspI CTAG 4 cut(s) 266, 459, 641, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.