RchiOBHm_Chr7g0188521

DnaJ central domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006961
Physical Location & Seq
Forward (+)
8002259 .. 8002819
561 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 561 bp
ATGGATTGGCAGAGAGCTCCAAAGAAAAGCGATAGCCGTAGGAACTATTATGACATCCTTGAAGTGCCTAAGAATTCTTCACAAGATGATCTGAAAAAGGCTTCTAGAAGAGCAGCCATGAAGCACCATCCCGATAAGGGTGGTGATGTAGAAAAGTTCAAAGAGTTGGGCCATGCATACAAGGTTCTGTGCGATCCACAGCAACGTGTCATCTATGACCGGGACGGTGAGGATCAGTTTGGCGAGGACATGTACGGTGAGGACCGGTATGGCAAGGACCAATACGGTGAGTACAAGTACAGCCAGAACAGATACGGTCAGGACTGGTACTGGTACTGGTACTGGTACGGTTCGAACCAATACGGTCAGGACTGGTACGGGAATCGGTACGACCAGGGCTGGTACGGCCAGAACCGGTATGGTAAGGAAAAGTATAGCAAGGACCGAGATCAGACTGGCGAGCAAAGGGATGTAGTTAGACTAATCTGGTCCCTAGGTTTGATGGTTATTATTAGTTTGATTATTAAGTTATTTTTTTTGGGTGAATACTTTGATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

186

Amino Acids

22.66

Weight (kDa)

6.32

Isoelectric Point (pI)

25.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 15 - 73 1.3e-19 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 188, 240
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 1 cut(s) 73
AfiI CCNNNNNNNGG 1 cut(s) 137
AflIII ACRYGT 2 cut(s) 205, 249
AgeI ACCGGT 2 cut(s) 264, 414
AgsI TTSAA 2 cut(s) 62, 160
AjnI CCWGG 1 cut(s) 393
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
Alw21I GWGCWC 1 cut(s) 19
AlwI GGATC 2 cut(s) 188, 240
AoxI GGCC 2 cut(s) 169, 406
ApeKI GCWGC 1 cut(s) 113
ApoI RAATTY 1 cut(s) 73
AsiGI ACCGGT 2 cut(s) 264, 414
AspA2I CCTAGG 1 cut(s) 493
AspS9I GGNCC 5 cut(s) 169, 262, 277, 442, 489
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 5 cut(s) 155, 239, 269, 299, 554
AsuII TTCGAA 1 cut(s) 353
AvaII GGWCC 4 cut(s) 262, 277, 442, 489
AvrII CCTAGG 1 cut(s) 493
BanII GRGCYC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 1 cut(s) 125
BccI CCATC 2 cut(s) 135, 496
BceAI ACGGC 2 cut(s) 21, 421
BciT130I CCWGG 1 cut(s) 395
BcnI CCSGG 1 cut(s) 221
BfaI CTAG 2 cut(s) 105, 494
BisI GCNGC 1 cut(s) 114
BlnI CCTAGG 1 cut(s) 493
BlsI GCNGC 1 cut(s) 115
Bme1390I CCNGG 2 cut(s) 221, 395
Bme18I GGWCC 4 cut(s) 262, 277, 442, 489
BmgT120I GGNCC 5 cut(s) 169, 262, 277, 442, 489
BmiI GGNNCC 1 cut(s) 491
BmrFI CCNGG 2 cut(s) 221, 395
Bpu14I TTCGAA 1 cut(s) 353
BpuMI CCSGG 1 cut(s) 221
BsaJI CCNNGG 2 cut(s) 394, 493
BsaWI WCCGGW 2 cut(s) 264, 414
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse118I RCCGGY 2 cut(s) 264, 414
Bse1I ACTGG 6 cut(s) 329, 335, 341, 347, 377, 460
BseBI CCWGG 1 cut(s) 395
BseDI CCNNGG 2 cut(s) 394, 493
BseGI GGATG 3 cut(s) 54, 127, 475
BseLI CCNNNNNNNGG 1 cut(s) 137
BseNI ACTGG 6 cut(s) 329, 335, 341, 347, 377, 460
BseXI GCAGC 1 cut(s) 125
BshFI GGCC 2 cut(s) 171, 408
BshTI ACCGGT 2 cut(s) 264, 414
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 3 cut(s) 220, 265, 415
BslFI GGGAC 2 cut(s) 236, 475
BslI CCNNNNNNNGG 1 cut(s) 137
BsmFI GGGAC 2 cut(s) 236, 475
BsnI GGCC 2 cut(s) 171, 408
Bsp119I TTCGAA 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 4 cut(s) 88, 193, 232, 448
BspANI GGCC 2 cut(s) 171, 408
BspLI GGNNCC 1 cut(s) 491
BspPI GGATC 2 cut(s) 188, 240
BspQI GCTCTTC 1 cut(s) 103
BspT104I TTCGAA 1 cut(s) 353
BsrFI RCCGGY 2 cut(s) 264, 414
BsrI ACTGG 6 cut(s) 329, 335, 341, 347, 377, 460
BssAI RCCGGY 2 cut(s) 264, 414
BssECI CCNNGG 2 cut(s) 394, 493
BssMI GATC 4 cut(s) 88, 193, 232, 448
BssT1I CCWWGG 1 cut(s) 493
Bst2UI CCWGG 1 cut(s) 395
Bst4CI ACNGT 6 cut(s) 227, 257, 287, 317, 350, 365
Bst6I CTCTTC 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 461
BstDEI CTNAG 1 cut(s) 69
BstF5I GGATG 3 cut(s) 54, 127, 475
BstKTI GATC 4 cut(s) 91, 196, 235, 451
BstMBI GATC 4 cut(s) 88, 193, 232, 448
BstMWI GCNNNNNNNGC 1 cut(s) 405
BstNI CCWGG 1 cut(s) 395
BstNSI RCATGY 1 cut(s) 253
BstSCI CCNGG 2 cut(s) 219, 393
BstV1I GCAGC 1 cut(s) 125
BsuRI GGCC 2 cut(s) 171, 408
BtsCI GGATG 3 cut(s) 54, 127, 475
Cac8I GCNNGC 1 cut(s) 461
Cfr10I RCCGGY 2 cut(s) 264, 414
Cfr13I GGNCC 5 cut(s) 169, 262, 277, 442, 489
CspAI ACCGGT 2 cut(s) 264, 414
CviAII CATG 3 cut(s) 118, 173, 250
CviJI RGCY 8 cut(s) 17, 36, 101, 116, 171, 303, 399, 408
CviKI_1 RGCY 8 cut(s) 17, 36, 101, 116, 171, 303, 399, 408
DdeI CTNAG 1 cut(s) 69
DpnI GATC 4 cut(s) 90, 195, 234, 450
DpnII GATC 4 cut(s) 88, 193, 232, 448
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 19
Eco47I GGWCC 4 cut(s) 262, 277, 442, 489
Eco53kI GAGCTC 1 cut(s) 17
EcoICRI GAGCTC 1 cut(s) 17
EcoRI GAATTC 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 393
EcoT14I CCWWGG 1 cut(s) 493
EcoT22I ATGCAT 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 493
FaeI CATG 3 cut(s) 121, 176, 253
FaiI YATR 9 cut(s) 51, 119, 174, 178, 216, 251, 270, 420, 435
FaqI GGGAC 2 cut(s) 236, 475
FatI CATG 3 cut(s) 117, 172, 249
Fnu4HI GCNGC 1 cut(s) 114
FokI GGATG 3 cut(s) 41, 114, 482
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 1 cut(s) 114
FspBI CTAG 2 cut(s) 105, 494
GluI GCNGC 1 cut(s) 114
HaeIII GGCC 2 cut(s) 171, 408
HapII CCGG 3 cut(s) 220, 265, 415
Hin1II CATG 3 cut(s) 121, 176, 253
HinfI GANTC 1 cut(s) 382
HpaII CCGG 3 cut(s) 220, 265, 415
HphI GGTGA 5 cut(s) 155, 239, 269, 299, 554
Hpy188I TCNGA 2 cut(s) 93, 453
Hpy188III TCNNGA 4 cut(s) 105, 131, 320, 368
HpyCH4III ACNGT 6 cut(s) 227, 257, 287, 317, 350, 365
HpyCH4IV ACGT 1 cut(s) 205
HpyCH4V TGCA 1 cut(s) 176
HpyF10VI GCNNNNNNNGC 1 cut(s) 405
HpyF3I CTNAG 1 cut(s) 69
HpySE526I ACGT 1 cut(s) 205
Hsp92II CATG 3 cut(s) 121, 176, 253
Kzo9I GATC 4 cut(s) 88, 193, 232, 448
LguI GCTCTTC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 22
Lsp1109I GCAGC 1 cut(s) 125
MaeI CTAG 2 cut(s) 105, 494
MaeII ACGT 1 cut(s) 205
MalI GATC 4 cut(s) 90, 195, 234, 450
MboI GATC 4 cut(s) 88, 193, 232, 448
MboII GAAGA 2 cut(s) 69, 120
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 1 cut(s) 73
MnlI CCTC 3 cut(s) 223, 238, 253
Mph1103I ATGCAT 1 cut(s) 178
MseI TTAA 2 cut(s) 525, 559
MspI CCGG 3 cut(s) 220, 265, 415
MspR9I CCNGG 2 cut(s) 221, 395
MvaI CCWGG 1 cut(s) 395
MwoI GCNNNNNNNGC 1 cut(s) 405
NciI CCSGG 1 cut(s) 221
NdeII GATC 4 cut(s) 88, 193, 232, 448
NlaIII CATG 3 cut(s) 121, 176, 253
NlaIV GGNNCC 1 cut(s) 491
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 1 cut(s) 253
NspV TTCGAA 1 cut(s) 353
PciI ACATGT 1 cut(s) 249
PciSI GCTCTTC 1 cut(s) 103
PfeI GAWTC 1 cut(s) 382
PinAI ACCGGT 2 cut(s) 264, 414
PkrI GCNGC 1 cut(s) 115
PscI ACATGT 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 393
PspGI CCWGG 1 cut(s) 393
PspN4I GGNNCC 1 cut(s) 491
PspPI GGNCC 5 cut(s) 169, 262, 277, 442, 489
SacI GAGCTC 1 cut(s) 19
SapI GCTCTTC 1 cut(s) 103
SaqAI TTAA 2 cut(s) 525, 559
SatI GCNGC 1 cut(s) 114
Sau3AI GATC 4 cut(s) 88, 193, 232, 448
Sau96I GGNCC 5 cut(s) 169, 262, 277, 442, 489
ScrFI CCNGG 2 cut(s) 221, 395
SduI GDGCHC 1 cut(s) 19
SetI ASST 4 cut(s) 19, 186, 208, 499
SfuI TTCGAA 1 cut(s) 353
SinI GGWCC 4 cut(s) 262, 277, 442, 489
Sse9I AATT 1 cut(s) 73
SspMI CTAG 2 cut(s) 105, 494
SstI GAGCTC 1 cut(s) 19
StyD4I CCNGG 2 cut(s) 219, 393
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 6 cut(s) 227, 257, 287, 317, 350, 365
TaiI ACGT 1 cut(s) 208
TaqI TCGA 1 cut(s) 353
TaqII GACCGA 1 cut(s) 459
TasI AATT 1 cut(s) 73
TatI WGTACW 2 cut(s) 291, 297
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 2 cut(s) 525, 559
Tru9I TTAA 2 cut(s) 525, 559
TseI GCWGC 1 cut(s) 113
TspDTI ATGAA 1 cut(s) 134
VpaK11BI GGWCC 4 cut(s) 262, 277, 442, 489
XapI RAATTY 1 cut(s) 73
XbaI TCTAGA 1 cut(s) 104
XceI RCATGY 1 cut(s) 253
XcmI CCANNNNNNNNNTGG 1 cut(s) 416
XmaJI CCTAGG 1 cut(s) 493
XspI CTAG 2 cut(s) 105, 494
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.