RLG00000034231

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006961
Physical Location & Seq
Reverse (-)
38890927 .. 38892892
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034231

Sequence Viewer

Length: 528 bp
ATGTCTCCCTTTCACTTCACCTTCCGCTCCATCCTAAAATCCCACCACCACTACCATGACGTCAGCCACCAAACCTTCATGGACACCATCGTAATTAAACGGGTTCGCGACCGTGTCCTCGACCACCTGGTCGAGATTATGAGAGAGAGAGAGAGAGAGCTCTGGCCAATCATCAACATCAAGAACCTCATCAAACCAGAGCCTTCCAAATCCTTCCCCATTTCCACCCTCACCAAAACCCGACACTTCCTCATGATTCCCACGCGCCTCGTCGAGTTTGTTTGCCAATACCCCTCTATTTTTGAGGAATTCATGCTCGCCGGCATCATTGTTCAGCCTCACGTCCGCCTCACCCCGCAAGTCCTCGATATGTTATCGTCGATTGAGAAAGACTCAAATCCTCTGTCGGCTTCAGATGGCCCCAAAACTGCCGATCGACCGTTCGGGTCGGGTTTGGGTATGCCTTTGCTCCGTCAAAGGACTAGGTCGGCTGCCAGATTGATCAATGGCATGGATCGCCTATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

176

Amino Acids

20.3

Weight (kDa)

9.83

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 35 - 127 5.5e-13 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AatII GACGTC 1 cut(s) 63
AccBSI CCGCTC 1 cut(s) 27
AccII CGCG 2 cut(s) 108, 265
AciI CCGC 3 cut(s) 25, 346, 356
AclWI GGATC 1 cut(s) 522
AcoI YGGCCR 1 cut(s) 164
AcsI RAATTY 1 cut(s) 308
AcuI CTGAAG 1 cut(s) 396
AcyI GRCGYC 1 cut(s) 60
AjiI CACGTC 1 cut(s) 343
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 1 cut(s) 160
AluI AGCT 1 cut(s) 160
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 522
AoxI GGCC 2 cut(s) 164, 418
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 1 cut(s) 308
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 419
AsuHPI GGTGA 3 cut(s) 10, 223, 343
BalI TGGCCA 1 cut(s) 166
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 478
BccI CCATC 3 cut(s) 38, 95, 410
BciT130I CCWGG 1 cut(s) 128
BclI TGATCA 1 cut(s) 501
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 483
BisI GCNGC 1 cut(s) 492
BlsI GCNGC 1 cut(s) 493
Bme1390I CCNGG 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 1 cut(s) 419
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 377, 409
BsaHI GRCGYC 1 cut(s) 60
Bse118I RCCGGY 1 cut(s) 320
BseBI CCWGG 1 cut(s) 128
BseGI GGATG 1 cut(s) 30
BseXI GCAGC 1 cut(s) 478
Bsh1236I CGCG 2 cut(s) 108, 265
Bsh1285I CGRYCG 3 cut(s) 112, 436, 440
BshFI GGCC 2 cut(s) 166, 420
BsiEI CGRYCG 3 cut(s) 112, 436, 440
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 1 cut(s) 321
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 2 cut(s) 166, 420
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 3 cut(s) 433, 501, 514
Bsp68I TCGCGA 1 cut(s) 108
BspACI CCGC 3 cut(s) 25, 346, 356
BspANI GGCC 2 cut(s) 166, 420
BspFNI CGCG 2 cut(s) 108, 265
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 421
BspPI GGATC 1 cut(s) 522
BsrBI CCGCTC 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 320
BssAI RCCGGY 1 cut(s) 320
BssMI GATC 3 cut(s) 433, 501, 514
BssNI GRCGYC 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 2 cut(s) 113, 441
BstACI GRCGYC 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 318, 322
BstF5I GGATG 1 cut(s) 30
BstFNI CGCG 2 cut(s) 108, 265
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 3 cut(s) 436, 504, 517
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 3 cut(s) 433, 501, 514
BstMCI CGRYCG 3 cut(s) 112, 436, 440
BstMWI GCNNNNNNNGC 1 cut(s) 516
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 126
BstUI CGCG 2 cut(s) 108, 265
BstV1I GCAGC 1 cut(s) 478
BsuRI GGCC 2 cut(s) 166, 420
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 1 cut(s) 30
BtuMI TCGCGA 1 cut(s) 108
Cac8I GCNNGC 2 cut(s) 318, 322
CciI TCATGA 1 cut(s) 252
CfoI GCGC 1 cut(s) 267
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 1 cut(s) 419
CsiI ACCWGGT 1 cut(s) 126
CviAII CATG 5 cut(s) 56, 79, 253, 313, 511
CviJI RGCY 8 cut(s) 66, 160, 166, 202, 337, 410, 420, 491
CviKI_1 RGCY 8 cut(s) 66, 160, 166, 202, 337, 410, 420, 491
DpnI GATC 3 cut(s) 435, 503, 516
DpnII GATC 3 cut(s) 433, 501, 514
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 1 cut(s) 164
EciI GGCGGA 1 cut(s) 335
Ecl136II GAGCTC 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 160
EcoRI GAATTC 1 cut(s) 308
EcoRII CCWGG 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 162
FaeI CATG 5 cut(s) 59, 82, 256, 316, 514
FaiI YATR 8 cut(s) 57, 80, 140, 254, 314, 371, 461, 512
FatI CATG 5 cut(s) 55, 78, 252, 312, 510
FauI CCCGC 1 cut(s) 363
FbaI TGATCA 1 cut(s) 501
Fnu4HI GCNGC 1 cut(s) 492
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 1 cut(s) 492
FspBI CTAG 1 cut(s) 483
GlaI GCGC 1 cut(s) 266
GluI GCNGC 1 cut(s) 492
HaeIII GGCC 2 cut(s) 166, 420
HapII CCGG 1 cut(s) 321
HhaI GCGC 1 cut(s) 267
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 5 cut(s) 59, 82, 256, 316, 514
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HinfI GANTC 2 cut(s) 256, 392
HpaII CCGG 1 cut(s) 321
HphI GGTGA 3 cut(s) 10, 223, 343
Hpy188I TCNGA 1 cut(s) 415
Hpy188III TCNNGA 5 cut(s) 107, 133, 181, 253, 525
Hpy99I CGWCG 2 cut(s) 275, 382
HpyAV CCTTC 4 cut(s) 31, 85, 213, 223
HpyCH4III ACNGT 2 cut(s) 113, 441
HpyCH4IV ACGT 2 cut(s) 60, 342
HpyF10VI GCNNNNNNNGC 1 cut(s) 516
HpySE526I ACGT 2 cut(s) 60, 342
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 5 cut(s) 59, 82, 256, 316, 514
HspAI GCGC 1 cut(s) 265
KroI GCCGGC 1 cut(s) 320
KroNI GCCGGC 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 501
Kzo9I GATC 3 cut(s) 433, 501, 514
LmnI GCTCC 2 cut(s) 32, 474
LpnPI CCDG 6 cut(s) 113, 140, 148, 210, 334, 508
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 1 cut(s) 333
MabI ACCWGGT 1 cut(s) 126
MaeI CTAG 1 cut(s) 483
MaeII ACGT 2 cut(s) 60, 342
MalI GATC 3 cut(s) 435, 503, 516
MbiI CCGCTC 1 cut(s) 27
MboI GATC 3 cut(s) 433, 501, 514
MhlI GDGCHC 1 cut(s) 162
MlsI TGGCCA 1 cut(s) 166
MluCI AATT 2 cut(s) 93, 308
MluNI TGGCCA 1 cut(s) 166
MlyI GAGTC 1 cut(s) 386
Mox20I TGGCCA 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 320
MscI TGGCCA 1 cut(s) 166
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 54
Msp20I TGGCCA 1 cut(s) 166
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 128
MvaI CCWGG 1 cut(s) 128
MvnI CGCG 2 cut(s) 108, 265
MwoI GCNNNNNNNGC 1 cut(s) 516
NaeI GCCGGC 1 cut(s) 322
NdeII GATC 3 cut(s) 433, 501, 514
NgoMIV GCCGGC 1 cut(s) 320
NlaIII CATG 5 cut(s) 59, 82, 256, 316, 514
NlaIV GGNNCC 1 cut(s) 421
NruI TCGCGA 1 cut(s) 108
PagI TCATGA 1 cut(s) 252
PdiI GCCGGC 1 cut(s) 322
PfeI GAWTC 1 cut(s) 256
PflFI GACNNNGTC 2 cut(s) 113, 484
PkrI GCNGC 1 cut(s) 493
Ple19I CGATCG 1 cut(s) 436
PleI GAGTC 1 cut(s) 386
PpsI GAGTC 1 cut(s) 386
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 1 cut(s) 419
PsyI GACNNNGTC 2 cut(s) 113, 484
PvuI CGATCG 1 cut(s) 436
RruI TCGCGA 1 cut(s) 108
RseI CAYNNNNRTG 1 cut(s) 54
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 3 cut(s) 433, 501, 514
Sau96I GGNCC 1 cut(s) 419
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 1 cut(s) 162
SetI ASST 8 cut(s) 23, 63, 77, 129, 162, 189, 345, 488
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 54
Sse9I AATT 2 cut(s) 93, 308
SsiI CCGC 3 cut(s) 25, 346, 356
SspMI CTAG 1 cut(s) 483
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 113, 441
TaiI ACGT 2 cut(s) 63, 345
TaqI TCGA 6 cut(s) 120, 132, 273, 366, 380, 436
TasI AATT 2 cut(s) 93, 308
TfiI GAWTC 1 cut(s) 256
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TseI GCWGC 1 cut(s) 491
TspDTI ATGAA 2 cut(s) 67, 301
TspGWI ACGGA 1 cut(s) 461
Tth111I GACNNNGTC 2 cut(s) 113, 484
XapI RAATTY 1 cut(s) 308
XspI CTAG 1 cut(s) 483
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.