RLG00000004984

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006961
Physical Location & Seq
Forward (+)
64175000 .. 64176097
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004984

Sequence Viewer

Length: 585 bp
ATGGACGCCACCGTAATTAAACTGAGTTCGCGACCGCATCCTCGACCACCTGGTCGAGAGAGAGAGAAAGAGAGAGAGCTCTGGCCAATCATCAACATCAAGAACCTTATCAAATCAGAGCCTTCCAAATCCCTCCCCATTTCCATTCTCACCAAAACCCGACACTTCCTCATGATTCTCACGCGCCCCGTCGAGTTTGTCTGCCAATACCCCTCTATTTTTGAGGAATTCCTGCTCGCCGACATCGTTGTTCAGCCTCATGTCCGCCTCACCCCGCAAGTCCTCAATCTGTTACCGTTGATTGAGAAAAACTCAAATCCTCTGCCAACTTCAGATGGCGCCAAAACCACCGACTGGCCGTTTGGGTCTGGTTTGGGTATGCCTCTGCTCTGTCAAAGGACTAGGGTGGCTACCAGGTTCTATTCCGGCGCGTCAATATGGAAGAAGTTGAGGTGGCCGACGATTAGAAGTGGCCGGGTCTGGTTCGACTTGGTTTTGGTTCAAATCCGATCTCTTGCCGGAATGGAGTTTGGGTCGAACGTCGGCAGCGTCTGGAGAATTTGGGTCATTGACGTCGGCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

195

Amino Acids

22.15

Weight (kDa)

10.13

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 22 - 98 7.3e-11 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 51, 578
AatII GACGTC 1 cut(s) 576
AccB1I GGYRCC 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 354
AccII CGCG 3 cut(s) 31, 184, 431
AciI CCGC 3 cut(s) 35, 265, 275
AcoI YGGCCR 4 cut(s) 83, 356, 455, 472
AcsI RAATTY 2 cut(s) 227, 558
AcuI CTGAAG 1 cut(s) 315
AcyI GRCGYC 4 cut(s) 6, 339, 573, 579
AfiI CCNNNNNNNGG 1 cut(s) 354
AgsI TTSAA 1 cut(s) 503
AjnI CCWGG 2 cut(s) 49, 413
AluBI AGCT 1 cut(s) 79
AluI AGCT 1 cut(s) 79
Alw21I GWGCWC 1 cut(s) 81
AlwNI CAGNNNCTG 1 cut(s) 552
AoxI GGCC 4 cut(s) 83, 356, 455, 472
ApeKI GCWGC 1 cut(s) 546
ApoI RAATTY 2 cut(s) 227, 558
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AspLEI GCGC 3 cut(s) 186, 341, 431
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 2 cut(s) 142, 262
BalI TGGCCA 1 cut(s) 85
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 558
BccI CCATC 1 cut(s) 329
BceAI ACGGC 1 cut(s) 343
BciT130I CCWGG 2 cut(s) 51, 415
BcnI CCSGG 1 cut(s) 476
BfaI CTAG 1 cut(s) 402
BfoI RGCGCY 1 cut(s) 342
BisI GCNGC 1 cut(s) 547
BlsI GCNGC 1 cut(s) 548
Bme1390I CCNGG 3 cut(s) 51, 415, 476
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 3 cut(s) 51, 415, 476
BmsI GCATC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 296, 328
BpmI CTGGAG 1 cut(s) 574
BpuMI CCSGG 1 cut(s) 476
BsaHI GRCGYC 4 cut(s) 6, 339, 573, 579
BsaXI ACNNNNNCTCC 2 cut(s) 518, 548
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse1I ACTGG 1 cut(s) 359
BseBI CCWGG 2 cut(s) 51, 415
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 1 cut(s) 359
BseXI GCAGC 1 cut(s) 558
Bsh1236I CGCG 3 cut(s) 31, 184, 431
Bsh1285I CGRYCG 1 cut(s) 35
BshFI GGCC 4 cut(s) 85, 358, 457, 474
BshNI GGYRCC 1 cut(s) 338
BsiEI CGRYCG 1 cut(s) 35
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 3 cut(s) 426, 475, 519
BslI CCNNNNNNNGG 1 cut(s) 354
BsnI GGCC 4 cut(s) 85, 358, 457, 474
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 1 cut(s) 509
Bsp68I TCGCGA 1 cut(s) 31
BspACI CCGC 3 cut(s) 35, 265, 275
BspANI GGCC 4 cut(s) 85, 358, 457, 474
BspCNI CTCAG 1 cut(s) 15
BspFNI CGCG 3 cut(s) 31, 184, 431
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 1 cut(s) 340
BspT107I GGYRCC 1 cut(s) 338
BsrI ACTGG 1 cut(s) 359
BssMI GATC 1 cut(s) 509
BssNI GRCGYC 4 cut(s) 6, 339, 573, 579
Bst2UI CCWGG 2 cut(s) 51, 415
Bst4CI ACNGT 2 cut(s) 13, 297
BstACI GRCGYC 4 cut(s) 6, 339, 573, 579
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 1 cut(s) 23
BstF5I GGATG 1 cut(s) 37
BstFNI CGCG 3 cut(s) 31, 184, 431
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 3 cut(s) 186, 341, 431
BstKTI GATC 1 cut(s) 512
BstMBI GATC 1 cut(s) 509
BstMCI CGRYCG 1 cut(s) 35
BstNI CCWGG 2 cut(s) 51, 415
BstSCI CCNGG 3 cut(s) 49, 413, 474
BstUI CGCG 3 cut(s) 31, 184, 431
BstV1I GCAGC 1 cut(s) 558
BsuRI GGCC 4 cut(s) 85, 358, 457, 474
BtsCI GGATG 1 cut(s) 37
BtuMI TCGCGA 1 cut(s) 31
Cac8I GCNNGC 1 cut(s) 237
CaiI CAGNNNCTG 1 cut(s) 552
CciI TCATGA 1 cut(s) 171
CfoI GCGC 3 cut(s) 186, 341, 431
CseI GACGC 4 cut(s) 14, 420, 538, 568
CsiI ACCWGGT 2 cut(s) 49, 413
CviAII CATG 2 cut(s) 172, 260
CviJI RGCY 8 cut(s) 79, 85, 121, 256, 358, 410, 457, 474
CviKI_1 RGCY 8 cut(s) 79, 85, 121, 256, 358, 410, 457, 474
DdeI CTNAG 1 cut(s) 23
DinI GGCGCC 1 cut(s) 340
DpnI GATC 1 cut(s) 511
DpnII GATC 1 cut(s) 509
DrdI GACNNNNNNGTC 2 cut(s) 51, 578
DseDI GACNNNNNNGTC 2 cut(s) 51, 578
EaeI YGGCCR 4 cut(s) 83, 356, 455, 472
EciI GGCGGA 1 cut(s) 254
Ecl136II GAGCTC 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 81
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 79
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 49, 413
EcoT38I GRGCYC 1 cut(s) 81
EgeI GGCGCC 1 cut(s) 340
EheI GGCGCC 1 cut(s) 340
FaeI CATG 2 cut(s) 175, 263
FaiI YATR 4 cut(s) 173, 261, 380, 439
FatI CATG 2 cut(s) 171, 259
FauI CCCGC 1 cut(s) 282
Fnu4HI GCNGC 1 cut(s) 547
FokI GGATG 1 cut(s) 24
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 1 cut(s) 547
FspBI CTAG 1 cut(s) 402
GlaI GCGC 3 cut(s) 185, 340, 430
GluI GCNGC 1 cut(s) 547
GsuI CTGGAG 1 cut(s) 574
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 4 cut(s) 85, 358, 457, 474
HapII CCGG 3 cut(s) 426, 475, 519
HgaI GACGC 4 cut(s) 14, 420, 538, 568
HhaI GCGC 3 cut(s) 186, 341, 431
Hin1I GRCGYC 4 cut(s) 6, 339, 573, 579
Hin1II CATG 2 cut(s) 175, 263
Hin6I GCGC 3 cut(s) 184, 339, 429
HinP1I GCGC 3 cut(s) 184, 339, 429
HinfI GANTC 1 cut(s) 175
HpaII CCGG 3 cut(s) 426, 475, 519
HphI GGTGA 2 cut(s) 142, 262
Hpy188I TCNGA 4 cut(s) 118, 334, 509, 584
Hpy188III TCNNGA 5 cut(s) 30, 56, 100, 172, 553
Hpy99I CGWCG 4 cut(s) 194, 463, 545, 578
HpyAV CCTTC 1 cut(s) 132
HpyCH4III ACNGT 2 cut(s) 13, 297
HpyCH4IV ACGT 2 cut(s) 540, 573
HpyF3I CTNAG 1 cut(s) 23
HpySE526I ACGT 2 cut(s) 540, 573
Hsp92I GRCGYC 4 cut(s) 6, 339, 573, 579
Hsp92II CATG 2 cut(s) 175, 263
HspAI GCGC 3 cut(s) 184, 339, 429
KasI GGCGCC 1 cut(s) 338
Kzo9I GATC 1 cut(s) 509
Lsp1109I GCAGC 1 cut(s) 558
LweI GCATC 1 cut(s) 46
MabI ACCWGGT 2 cut(s) 49, 413
MaeI CTAG 1 cut(s) 402
MaeII ACGT 2 cut(s) 540, 573
MaeIII GTNAC 1 cut(s) 291
MalI GATC 1 cut(s) 511
MboI GATC 1 cut(s) 509
MboII GAAGA 1 cut(s) 454
MhlI GDGCHC 1 cut(s) 81
MlsI TGGCCA 1 cut(s) 85
MluCI AATT 3 cut(s) 15, 227, 558
MluNI TGGCCA 1 cut(s) 85
Mly113I GGCGCC 1 cut(s) 339
Mox20I TGGCCA 1 cut(s) 85
MscI TGGCCA 1 cut(s) 85
MseI TTAA 1 cut(s) 18
Msp20I TGGCCA 1 cut(s) 85
MspI CCGG 3 cut(s) 426, 475, 519
MspR9I CCNGG 3 cut(s) 51, 415, 476
MvaI CCWGG 2 cut(s) 51, 415
MvnI CGCG 3 cut(s) 31, 184, 431
NarI GGCGCC 1 cut(s) 339
NciI CCSGG 1 cut(s) 476
NdeII GATC 1 cut(s) 509
NlaIII CATG 2 cut(s) 175, 263
NlaIV GGNNCC 1 cut(s) 340
NruI TCGCGA 1 cut(s) 31
PagI TCATGA 1 cut(s) 171
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 546
PfeI GAWTC 1 cut(s) 175
PflMI CCANNNNNTGG 1 cut(s) 354
PkrI GCNGC 1 cut(s) 548
PluTI GGCGCC 1 cut(s) 342
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 2 cut(s) 49, 413
PspGI CCWGG 2 cut(s) 49, 413
PspN4I GGNNCC 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 552
RruI TCGCGA 1 cut(s) 31
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 1 cut(s) 547
Sau3AI GATC 1 cut(s) 509
ScrFI CCNGG 3 cut(s) 51, 415, 476
SduI GDGCHC 1 cut(s) 81
SetI ASST 7 cut(s) 52, 81, 108, 419, 455, 543, 576
SexAI ACCWGGT 2 cut(s) 49, 413
SfaNI GCATC 1 cut(s) 46
SfoI GGCGCC 1 cut(s) 340
Sse9I AATT 3 cut(s) 15, 227, 558
SsiI CCGC 3 cut(s) 35, 265, 275
SspDI GGCGCC 1 cut(s) 338
SspMI CTAG 1 cut(s) 402
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 3 cut(s) 49, 413, 474
TaaI ACNGT 2 cut(s) 13, 297
TaiI ACGT 2 cut(s) 543, 576
TaqI TCGA 5 cut(s) 43, 55, 192, 486, 536
TasI AATT 3 cut(s) 15, 227, 558
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TseI GCWGC 1 cut(s) 546
Van91I CCANNNNNTGG 1 cut(s) 354
XapI RAATTY 2 cut(s) 227, 558
XspI CTAG 1 cut(s) 402
ZraI GACGTC 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.