Rroxscaffold_3G00266340

DnaJ protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006961
Physical Location & Seq
Reverse (-)
59635964 .. 59637333
1370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00266340.1

Sequence Viewer

Length: 549 bp
ATGGATTGGCAGAGAGCTCCAAAGAAAAGCGATAGCCGTAGGAACTACTATGACATCCTTGAAGTGTCTAGGAATTCTTCACAAGATGATCTGAAAAAGGCTTATAGAAGAGCAGCCATGAAGCACCATCCCGATAAGGGTGGTGATGTAGAAAAGTTCAAAGAGTTGGGCCATGCATACAAGGTTCTGAGCGATCCACAGCAACGTGTCATCCATGATCGGGACGGTGAGGATCAGTTCGGCGAGGACATGTACGGTGAGGACCGGTACGGCAAGGACCAGTACAACAAGGACCAATACGGTGAGTACAAGTACAGCCAGAACAGATACGGTCAGGACTGGTACTGGTACGGTTCGAACCAATACGGTCAGGACTGGTACGGGAATCGGTACGACCAGGGCTGGTACGGCCAGAACCGGTATGGTAAGGGAAAGTATTGCAAGGACCGAGATCAGACTGGCGAGCAAAGGGATGTAGTTAGAGTAATCTGGTCCCTAGCCACATCAAAAGCTGCAAATTCAAGCTGGCCATGTATGCTATCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

182

Amino Acids

21.75

Weight (kDa)

8.32

Isoelectric Point (pI)

27.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 15 - 73 4.5e-21 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 188, 240
AcoI YGGCCR 2 cut(s) 409, 527
AcsI RAATTY 2 cut(s) 73, 517
AfiI CCNNNNNNNGG 2 cut(s) 137, 220
AflIII ACRYGT 2 cut(s) 205, 249
AgeI ACCGGT 2 cut(s) 264, 417
AgsI TTSAA 3 cut(s) 62, 160, 522
AjnI CCWGG 1 cut(s) 396
AluBI AGCT 3 cut(s) 17, 512, 525
AluI AGCT 3 cut(s) 17, 512, 525
Alw21I GWGCWC 1 cut(s) 19
AlwI GGATC 2 cut(s) 188, 240
AoxI GGCC 3 cut(s) 169, 409, 527
ApeKI GCWGC 2 cut(s) 113, 512
ApoI RAATTY 2 cut(s) 73, 517
AsiGI ACCGGT 2 cut(s) 264, 417
AspS9I GGNCC 6 cut(s) 169, 262, 277, 292, 445, 492
AsuHPI GGTGA 4 cut(s) 155, 239, 269, 314
AsuII TTCGAA 1 cut(s) 356
AvaII GGWCC 5 cut(s) 262, 277, 292, 445, 492
BalI TGGCCA 1 cut(s) 529
BanII GRGCYC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 2 cut(s) 125, 499
BccI CCATC 1 cut(s) 135
BceAI ACGGC 3 cut(s) 21, 286, 424
BciT130I CCWGG 1 cut(s) 398
BfaI CTAG 2 cut(s) 69, 497
BisI GCNGC 2 cut(s) 114, 513
BlsI GCNGC 2 cut(s) 115, 514
Bme1390I CCNGG 1 cut(s) 398
Bme18I GGWCC 5 cut(s) 262, 277, 292, 445, 492
BmgT120I GGNCC 6 cut(s) 169, 262, 277, 292, 445, 492
BmiI GGNNCC 1 cut(s) 494
BmrFI CCNGG 1 cut(s) 398
Bpu14I TTCGAA 1 cut(s) 356
BsaJI CCNNGG 1 cut(s) 397
BsaWI WCCGGW 2 cut(s) 264, 417
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 220
Bse118I RCCGGY 2 cut(s) 264, 417
Bse1I ACTGG 5 cut(s) 280, 344, 350, 380, 463
BseBI CCWGG 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 4 cut(s) 54, 127, 210, 478
BseLI CCNNNNNNNGG 2 cut(s) 137, 220
BseMII CTCAG 1 cut(s) 179
BseNI ACTGG 5 cut(s) 280, 344, 350, 380, 463
BseXI GCAGC 2 cut(s) 125, 499
BshFI GGCC 3 cut(s) 171, 411, 529
BshTI ACCGGT 2 cut(s) 264, 417
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 2 cut(s) 265, 418
BslFI GGGAC 2 cut(s) 236, 478
BslI CCNNNNNNNGG 2 cut(s) 137, 220
BsmFI GGGAC 2 cut(s) 236, 478
BsnI GGCC 3 cut(s) 171, 411, 529
Bsp119I TTCGAA 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 5 cut(s) 88, 193, 217, 232, 451
BspANI GGCC 3 cut(s) 171, 411, 529
BspCNI CTCAG 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 494
BspPI GGATC 2 cut(s) 188, 240
BspQI GCTCTTC 1 cut(s) 103
BspT104I TTCGAA 1 cut(s) 356
BsrFI RCCGGY 2 cut(s) 264, 417
BsrI ACTGG 5 cut(s) 280, 344, 350, 380, 463
BssAI RCCGGY 2 cut(s) 264, 417
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 5 cut(s) 88, 193, 217, 232, 451
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 6 cut(s) 227, 257, 302, 332, 353, 368
Bst6I CTCTTC 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 356
BstC8I GCNNGC 2 cut(s) 464, 527
BstDEI CTNAG 1 cut(s) 188
BstF5I GGATG 4 cut(s) 54, 127, 210, 478
BstKTI GATC 5 cut(s) 91, 196, 220, 235, 454
BstMBI GATC 5 cut(s) 88, 193, 217, 232, 451
BstMWI GCNNNNNNNGC 2 cut(s) 408, 535
BstNI CCWGG 1 cut(s) 398
BstNSI RCATGY 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 396
BstV1I GCAGC 2 cut(s) 125, 499
BsuRI GGCC 3 cut(s) 171, 411, 529
BtsCI GGATG 4 cut(s) 54, 127, 210, 478
Cac8I GCNNGC 2 cut(s) 464, 527
Cfr10I RCCGGY 2 cut(s) 264, 417
Cfr13I GGNCC 6 cut(s) 169, 262, 277, 292, 445, 492
CspAI ACCGGT 2 cut(s) 264, 417
CviAII CATG 5 cut(s) 118, 173, 215, 250, 531
DdeI CTNAG 1 cut(s) 188
DpnI GATC 5 cut(s) 90, 195, 219, 234, 453
DpnII GATC 5 cut(s) 88, 193, 217, 232, 451
EaeI YGGCCR 2 cut(s) 409, 527
Eam1104I CTCTTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 17
Eco24I GRGCYC 1 cut(s) 19
Eco47I GGWCC 5 cut(s) 262, 277, 292, 445, 492
Eco53kI GAGCTC 1 cut(s) 17
EcoICRI GAGCTC 1 cut(s) 17
EcoRI GAATTC 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 396
EcoT22I ATGCAT 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 19
FaeI CATG 5 cut(s) 121, 176, 218, 253, 534
FaqI GGGAC 2 cut(s) 236, 478
FatI CATG 5 cut(s) 117, 172, 214, 249, 530
Fnu4HI GCNGC 2 cut(s) 114, 513
FokI GGATG 4 cut(s) 41, 114, 197, 485
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 2 cut(s) 114, 513
FspBI CTAG 2 cut(s) 69, 497
GluI GCNGC 2 cut(s) 114, 513
HaeIII GGCC 3 cut(s) 171, 411, 529
HapII CCGG 2 cut(s) 265, 418
Hin1II CATG 5 cut(s) 121, 176, 218, 253, 534
HinfI GANTC 1 cut(s) 385
HpaII CCGG 2 cut(s) 265, 418
HphI GGTGA 4 cut(s) 155, 239, 269, 314
Hpy188I TCNGA 3 cut(s) 93, 189, 456
Hpy188III TCNNGA 4 cut(s) 131, 221, 335, 371
HpyCH4III ACNGT 6 cut(s) 227, 257, 302, 332, 353, 368
HpyCH4IV ACGT 1 cut(s) 205
HpyCH4V TGCA 3 cut(s) 176, 441, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 408, 535
HpyF3I CTNAG 1 cut(s) 188
HpySE526I ACGT 1 cut(s) 205
Hsp92II CATG 5 cut(s) 121, 176, 218, 253, 534
Kzo9I GATC 5 cut(s) 88, 193, 217, 232, 451
LguI GCTCTTC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 22
Lsp1109I GCAGC 2 cut(s) 125, 499
MaeI CTAG 2 cut(s) 69, 497
MaeII ACGT 1 cut(s) 205
MalI GATC 5 cut(s) 90, 195, 219, 234, 453
MboI GATC 5 cut(s) 88, 193, 217, 232, 451
MboII GAAGA 2 cut(s) 69, 120
MhlI GDGCHC 1 cut(s) 19
MlsI TGGCCA 1 cut(s) 529
MluCI AATT 2 cut(s) 73, 517
MluNI TGGCCA 1 cut(s) 529
MnlI CCTC 3 cut(s) 223, 238, 253
Mox20I TGGCCA 1 cut(s) 529
Mph1103I ATGCAT 1 cut(s) 178
MscI TGGCCA 1 cut(s) 529
MseI TTAA 1 cut(s) 547
Msp20I TGGCCA 1 cut(s) 529
MspI CCGG 2 cut(s) 265, 418
MspR9I CCNGG 1 cut(s) 398
MvaI CCWGG 1 cut(s) 398
MwoI GCNNNNNNNGC 2 cut(s) 408, 535
NdeII GATC 5 cut(s) 88, 193, 217, 232, 451
NlaIII CATG 5 cut(s) 121, 176, 218, 253, 534
NlaIV GGNNCC 1 cut(s) 494
NsiI ATGCAT 1 cut(s) 178
NspI RCATGY 1 cut(s) 253
NspV TTCGAA 1 cut(s) 356
PciI ACATGT 1 cut(s) 249
PciSI GCTCTTC 1 cut(s) 103
PfeI GAWTC 1 cut(s) 385
PinAI ACCGGT 2 cut(s) 264, 417
PkrI GCNGC 2 cut(s) 115, 514
PscI ACATGT 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 396
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 494
PspPI GGNCC 6 cut(s) 169, 262, 277, 292, 445, 492
SacI GAGCTC 1 cut(s) 19
SapI GCTCTTC 1 cut(s) 103
SaqAI TTAA 1 cut(s) 547
SatI GCNGC 2 cut(s) 114, 513
Sau3AI GATC 5 cut(s) 88, 193, 217, 232, 451
Sau96I GGNCC 6 cut(s) 169, 262, 277, 292, 445, 492
ScrFI CCNGG 1 cut(s) 398
SduI GDGCHC 1 cut(s) 19
SetI ASST 5 cut(s) 19, 186, 208, 514, 527
SfuI TTCGAA 1 cut(s) 356
SinI GGWCC 5 cut(s) 262, 277, 292, 445, 492
Sse9I AATT 2 cut(s) 73, 517
SspMI CTAG 2 cut(s) 69, 497
SstI GAGCTC 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 396
TaaI ACNGT 6 cut(s) 227, 257, 302, 332, 353, 368
TaiI ACGT 1 cut(s) 208
TaqI TCGA 1 cut(s) 356
TaqII GACCGA 1 cut(s) 462
TasI AATT 2 cut(s) 73, 517
TatI WGTACW 3 cut(s) 282, 306, 312
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 1 cut(s) 547
Tru9I TTAA 1 cut(s) 547
TseI GCWGC 2 cut(s) 113, 512
TspDTI ATGAA 1 cut(s) 134
VpaK11BI GGWCC 5 cut(s) 262, 277, 292, 445, 492
XapI RAATTY 2 cut(s) 73, 517
XceI RCATGY 1 cut(s) 253
XcmI CCANNNNNNNNNTGG 1 cut(s) 419
XspI CTAG 2 cut(s) 69, 497
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.